MD08G1237800.v1.1

Belongs to the peptidase S10 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0001027
Physical Location & Seq
Forward (+)
30494653 .. 30497898
3246 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1237800.v1.1.491

Sequence Viewer

Length: 1380 bp
ATGATGCAGCCTCGACGAGGGAGTGTCGTCGTCGTCGCCGTAGTCTTTGCAACTGTCATCCAAGCGTTTTCCACGACACACTCCCTCCAAGAAGCTGATAAAATCACAAGGTTGCCTGGTCAACCACAGGTCAACTTCCAGCAACATGCAGGATATGTAACTGTGGATGAAAAGCAAGAGAGATCTCTTTTTTACTACTTTGTTGAAGCACAAACTGATCCTGCTTCAAAGCCTCTTGTTCTTTGGCTCAATGGAGGTCCTGGTTGTTCATCTGTTGGAGTTGGAGCTTTCAACGAGAACGGACCTTTTCAACCAAGCGGAGACATTTTACTCAAGAACGATTTTAGTTGGAATAGAGAAGCAAATATGCTATACTTGGAGTCACCTGCAGGAGTAGGCTTCTCCTACTCAGCAAACAAATCATTCTATGGCTTCGTGAACGACGATATCACAGCACAACATAATCTCGAGTTCCTTCAGCGCTGGTTCGACGAATTCCCCGAGTATAGAAATAGGGATTTCTTTATCACAGGAGAGAGCTATGCAGGCCACTATGTTCCACAGCTTGCACAACTCATTATCCAATCAAAACCGAAGTTCAATCTGAAAGCAGTGGCAATTGGCAATCCTCTTTTCGAATTCAATACTGATATCAACTCACGAGCAGAGTACTGGTGGTCTCATGGATTAATATCAGATGCGACCTTCGAAAATTTGACTTCAGTCTGCAACTATTCTGAAATTTTTAGACAGGAGGTAATTCGTGGTGGTCCACTTATCCCTGCTTGTGATGCAGTAGCGAACCAATTTGAAAGAGAAGTCCCTGAACATTTGATCGACCAATATGACGTTACTCTCGATGTCTGTTTATCATCCGTTGAAGCTCAAGCTTTCCAACTGCGAAAAACAGCGAAGATAGATGTCTGTATCGAAGATGAAACAGTTTCATACTTAAACCGACCAGATGTACAGGAGGCGCTTCATGCACGAGTCACAAATTGGATAACTTGCAGCAACCGAGTTGTCATCCAGTATGACATGAAAAACAAGGAAACACCGATGCTTCCAGTTTTAGGTGAACTTGTCAAGTCTGGAATTCGGGTTTTGATTTACAGTGGAGATCAAGATTCTATTATTCCACTAATAAGTACACGGACACTGGTGAACGGATTGGCAAAGGACTTGGGGCTACAAACGAATGTGCCTTACAGAGTCTGGTTTGCAGAAAAACAGGTTGCTGGATGGACACAAGTATACAGTGACGCTTTATCATACGCTACCATAAGAGGAGCAGGCCATGAAGCTCCAATTTCGCAGCCAGAGAGATCATTACTTCTATTTACTTCATTTCTGACAGGAATGCCATTGCCAAAAGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_S10

Protein Analysis

460

Amino Acids

51.53

Weight (kDa)

5.11

Isoelectric Point (pI)

37.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S10 PF00450 39 - 452 5.2e-129 Serine carboxypeptidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 394
Acc36I ACCTGC 1 cut(s) 394
AccI GTMKAC 1 cut(s) 1254
AciI CCGC 1 cut(s) 318
AclWI GGATC 1 cut(s) 212
AcsI RAATTY 5 cut(s) 494, 638, 712, 741, 1095
AcuI CTGAAG 2 cut(s) 461, 705
AfaI GTAC 3 cut(s) 671, 969, 1150
AfeI AGCGCT 1 cut(s) 482
AfiI CCNNNNNNNGG 2 cut(s) 17, 1073
AgsI TTSAA 8 cut(s) 206, 228, 292, 311, 601, 643, 812, 881
AjnI CCWGG 2 cut(s) 115, 259
AluBI AGCT 7 cut(s) 95, 287, 540, 565, 884, 890, 1304
AluI AGCT 7 cut(s) 95, 287, 540, 565, 884, 890, 1304
Alw26I GTCTC 2 cut(s) 315, 684
AlwI GGATC 1 cut(s) 212
AlwNI CAGNNNCTG 1 cut(s) 1215
Ama87I CYCGRG 2 cut(s) 467, 500
Aor51HI AGCGCT 1 cut(s) 482
AoxI GGCC 2 cut(s) 547, 1294
ApeKI GCWGC 3 cut(s) 7, 1011, 1315
ApoI RAATTY 5 cut(s) 494, 638, 712, 741, 1095
AseI ATTAAT 1 cut(s) 689
AspLEI GCGC 2 cut(s) 483, 979
AspS9I GGNCC 3 cut(s) 257, 302, 770
AsuHPI GGTGA 3 cut(s) 375, 1088, 1174
AsuII TTCGAA 2 cut(s) 636, 708
AvaI CYCGRG 2 cut(s) 467, 500
AvaII GGWCC 3 cut(s) 257, 302, 770
BauI CACGAG 2 cut(s) 660, 987
BbvI GCAGC 3 cut(s) 19, 1023, 1327
BccI CCATC 1 cut(s) 1236
BceAI ACGGC 1 cut(s) 23
BciT130I CCWGG 2 cut(s) 117, 261
BcoDI GTCTC 2 cut(s) 315, 684
BfmI CTRYAG 1 cut(s) 387
BfoI RGCGCY 2 cut(s) 484, 980
BfuAI ACCTGC 1 cut(s) 394
BglII AGATCT 1 cut(s) 182
BisI GCNGC 3 cut(s) 8, 1012, 1316
BlsI GCNGC 3 cut(s) 9, 1013, 1317
BmcAI AGTACT 1 cut(s) 671
Bme1390I CCNGG 2 cut(s) 117, 261
Bme18I GGWCC 3 cut(s) 257, 302, 770
BmeT110I CYCGRG 2 cut(s) 467, 500
BmgT120I GGNCC 3 cut(s) 257, 302, 770
BmrFI CCNGG 2 cut(s) 117, 261
BmsI GCATC 3 cut(s) 688, 781, 1050
BoxI GACNNNNGTC 1 cut(s) 722
Bpu14I TTCGAA 2 cut(s) 636, 708
BpuEI CTTGAG 2 cut(s) 317, 870
BsaBI GATNNNNATC 1 cut(s) 691
BsaI GGTCTC 1 cut(s) 684
BsaXI ACNNNNNCTCC 2 cut(s) 69, 99
Bsc4I CCNNNNNNNGG 2 cut(s) 17, 1073
Bse1I ACTGG 4 cut(s) 677, 1030, 1067, 1164
Bse3DI GCAATG 1 cut(s) 1364
Bse8I GATNNNNATC 1 cut(s) 691
BseBI CCWGG 2 cut(s) 117, 261
BseGI GGATG 5 cut(s) 57, 172, 872, 1026, 1247
BseJI GATNNNNATC 1 cut(s) 691
BseLI CCNNNNNNNGG 2 cut(s) 17, 1073
BseMI GCAATG 1 cut(s) 1364
BseMII CTCAG 1 cut(s) 423
BseNI ACTGG 4 cut(s) 677, 1030, 1067, 1164
BseRI GAGGAG 1 cut(s) 1302
BseXI GCAGC 3 cut(s) 19, 1023, 1327
BshFI GGCC 2 cut(s) 549, 1296
BsiHKCI CYCGRG 2 cut(s) 467, 500
BslFI GGGAC 1 cut(s) 806
BslI CCNNNNNNNGG 2 cut(s) 17, 1073
BsmAI GTCTC 2 cut(s) 315, 684
BsmFI GGGAC 1 cut(s) 806
BsmI GAATGC 1 cut(s) 1365
BsnI GGCC 2 cut(s) 549, 1296
Bso31I GGTCTC 1 cut(s) 684
BsoBI CYCGRG 2 cut(s) 467, 500
Bsp119I TTCGAA 2 cut(s) 636, 708
Bsp1407I TGTACA 1 cut(s) 967
Bsp143I GATC 5 cut(s) 182, 217, 834, 1120, 1325
BspACI CCGC 1 cut(s) 318
BspANI GGCC 2 cut(s) 549, 1296
BspCNI CTCAG 1 cut(s) 422
BspMAI CTGCAG 1 cut(s) 391
BspMI ACCTGC 1 cut(s) 394
BspPI GGATC 1 cut(s) 212
BspT104I TTCGAA 2 cut(s) 636, 708
BspTNI GGTCTC 1 cut(s) 684
BsrDI GCAATG 1 cut(s) 1364
BsrGI TGTACA 1 cut(s) 967
BsrI ACTGG 4 cut(s) 677, 1030, 1067, 1164
BssMI GATC 5 cut(s) 182, 217, 834, 1120, 1325
BssNAI GTATAC 1 cut(s) 1255
BssSI CACGAG 2 cut(s) 660, 987
Bst1107I GTATAC 1 cut(s) 1255
Bst2BI CACGAG 2 cut(s) 660, 987
Bst2UI CCWGG 2 cut(s) 117, 261
Bst4CI ACNGT 5 cut(s) 55, 163, 943, 1115, 1259
BstAUI TGTACA 1 cut(s) 967
BstBI TTCGAA 2 cut(s) 636, 708
BstC8I GCNNGC 3 cut(s) 547, 567, 1294
BstDEI CTNAG 1 cut(s) 409
BstENI CCTNNNNNAGG 1 cut(s) 15
BstF5I GGATG 5 cut(s) 57, 172, 872, 1026, 1247
BstH2I RGCGCY 2 cut(s) 484, 980
BstHHI GCGC 2 cut(s) 483, 979
BstKTI GATC 5 cut(s) 185, 220, 837, 1123, 1328
BstMAI GTCTC 2 cut(s) 315, 684
BstMBI GATC 5 cut(s) 182, 217, 834, 1120, 1325
BstMWI GCNNNNNNNGC 3 cut(s) 546, 791, 983
BstNI CCWGG 2 cut(s) 117, 261
BstNSI RCATGY 1 cut(s) 149
BstPAI GACNNNNGTC 1 cut(s) 722
BstSCI CCNGG 2 cut(s) 115, 259
BstSFI CTRYAG 1 cut(s) 387
BstV1I GCAGC 3 cut(s) 19, 1023, 1327
BstX2I RGATCY 1 cut(s) 182
BstYI RGATCY 1 cut(s) 182
BstZ17I GTATAC 1 cut(s) 1255
BsuRI GGCC 2 cut(s) 549, 1296
BtsCI GGATG 5 cut(s) 57, 172, 872, 1026, 1247
BtsI GCAGTG 1 cut(s) 618
BtsIMutI CAGTG 4 cut(s) 618, 1120, 1157, 1264
BveI ACCTGC 1 cut(s) 394
Cac8I GCNNGC 3 cut(s) 547, 567, 1294
CaiI CAGNNNCTG 1 cut(s) 1215
CfoI GCGC 2 cut(s) 483, 979
Cfr13I GGNCC 3 cut(s) 257, 302, 770
CseI GACGC 1 cut(s) 1271
Csp6I GTAC 3 cut(s) 670, 968, 1149
CviAII CATG 5 cut(s) 146, 683, 983, 1039, 1298
CviQI GTAC 3 cut(s) 670, 968, 1149
DdeI CTNAG 1 cut(s) 409
DpnI GATC 5 cut(s) 184, 219, 836, 1122, 1327
DpnII GATC 5 cut(s) 182, 217, 834, 1120, 1325
Eco31I GGTCTC 1 cut(s) 684
Eco32I GATATC 2 cut(s) 448, 652
Eco47I GGWCC 3 cut(s) 257, 302, 770
Eco47III AGCGCT 1 cut(s) 482
Eco57I CTGAAG 2 cut(s) 461, 705
Eco88I CYCGRG 2 cut(s) 467, 500
EcoNI CCTNNNNNAGG 1 cut(s) 15
EcoO109I RGGNCCY 1 cut(s) 257
EcoRI GAATTC 3 cut(s) 494, 638, 1095
EcoRII CCWGG 2 cut(s) 115, 259
EcoRV GATATC 2 cut(s) 448, 652
FaeI CATG 5 cut(s) 149, 686, 986, 1042, 1301
FaqI GGGAC 1 cut(s) 806
FatI CATG 5 cut(s) 145, 682, 982, 1038, 1297
FblI GTMKAC 1 cut(s) 1254
Fnu4HI GCNGC 3 cut(s) 8, 1012, 1316
FokI GGATG 5 cut(s) 44, 179, 859, 1013, 1254
Fsp4HI GCNGC 3 cut(s) 8, 1012, 1316
GlaI GCGC 2 cut(s) 482, 978
GluI GCNGC 3 cut(s) 8, 1012, 1316
HaeII RGCGCY 2 cut(s) 484, 980
HaeIII GGCC 2 cut(s) 549, 1296
HgaI GACGC 1 cut(s) 1271
HhaI GCGC 2 cut(s) 483, 979
Hin1II CATG 5 cut(s) 149, 686, 986, 1042, 1301
Hin6I GCGC 2 cut(s) 481, 977
HinP1I GCGC 2 cut(s) 481, 977
HincII GTYRAC 2 cut(s) 122, 133
HindII GTYRAC 2 cut(s) 122, 133
HindIII AAGCTT 1 cut(s) 888
HinfI GANTC 4 cut(s) 380, 990, 1127, 1212
HphI GGTGA 3 cut(s) 375, 1088, 1174
Hpy166II GTNNAC 8 cut(s) 122, 133, 439, 773, 1079, 1151, 1165, 1255
Hpy188I TCNGA 4 cut(s) 606, 697, 739, 1353
Hpy188III TCNNGA 7 cut(s) 334, 436, 467, 660, 857, 1092, 1124
Hpy8I GTNNAC 8 cut(s) 122, 133, 439, 773, 1079, 1151, 1165, 1255
Hpy99I CGWCG 6 cut(s) 18, 32, 35, 38, 446, 494
HpyAV CCTTC 2 cut(s) 485, 715
HpyCH4III ACNGT 5 cut(s) 55, 163, 943, 1115, 1259
HpyCH4IV ACGT 1 cut(s) 849
HpyF10VI GCNNNNNNNGC 3 cut(s) 546, 791, 983
HpyF3I CTNAG 1 cut(s) 409
HpySE526I ACGT 1 cut(s) 849
Hsp92II CATG 5 cut(s) 149, 686, 986, 1042, 1301
HspAI GCGC 2 cut(s) 481, 977
Kzo9I GATC 5 cut(s) 182, 217, 834, 1120, 1325
LmnI GCTCC 3 cut(s) 284, 1289, 1309
Lsp1109I GCAGC 3 cut(s) 19, 1023, 1327
LweI GCATC 3 cut(s) 688, 781, 1050
MaeII ACGT 1 cut(s) 849
MaeIII GTNAC 5 cut(s) 157, 381, 850, 991, 1259
MalI GATC 5 cut(s) 184, 219, 836, 1122, 1327
MboI GATC 5 cut(s) 182, 217, 834, 1120, 1325
MboII GAAGA 2 cut(s) 925, 944
MfeI CAATTG 1 cut(s) 618
MflI RGATCY 1 cut(s) 182
MlyI GAGTC 3 cut(s) 389, 999, 1221
MmeI TCCRAC 4 cut(s) 256, 262, 329, 919
MnlI CCTC 9 cut(s) 11, 21, 95, 243, 248, 639, 748, 967, 1280
MseI TTAA 2 cut(s) 689, 953
MspR9I CCNGG 2 cut(s) 117, 261
MunI CAATTG 1 cut(s) 618
Mva1269I GAATGC 1 cut(s) 1365
MvaI CCWGG 2 cut(s) 117, 261
MwoI GCNNNNNNNGC 3 cut(s) 546, 791, 983
NdeII GATC 5 cut(s) 182, 217, 834, 1120, 1325
NlaIII CATG 5 cut(s) 149, 686, 986, 1042, 1301
NmuCI GTSAC 3 cut(s) 381, 991, 1259
NspI RCATGY 1 cut(s) 149
NspV TTCGAA 2 cut(s) 636, 708
PaeR7I CTCGAG 1 cut(s) 467
PaqCI CACCTGC 1 cut(s) 394
PcsI WCGNNNNNNNCGW 4 cut(s) 36, 441, 498, 855
PctI GAATGC 1 cut(s) 1365
PfeI GAWTC 1 cut(s) 1127
PkrI GCNGC 3 cut(s) 9, 1013, 1317
PleI GAGTC 3 cut(s) 388, 998, 1220
PpsI GAGTC 3 cut(s) 388, 998, 1220
PpuMI RGGWCCY 1 cut(s) 257
PshAI GACNNNNGTC 1 cut(s) 722
PshBI ATTAAT 1 cut(s) 689
Psp5II RGGWCCY 1 cut(s) 257
Psp6I CCWGG 2 cut(s) 115, 259
PspGI CCWGG 2 cut(s) 115, 259
PspPI GGNCC 3 cut(s) 257, 302, 770
PspPPI RGGWCCY 1 cut(s) 257
PstI CTGCAG 1 cut(s) 391
PstNI CAGNNNCTG 1 cut(s) 1215
PsuI RGATCY 1 cut(s) 182
RsaI GTAC 3 cut(s) 671, 969, 1150
RsaNI GTAC 3 cut(s) 670, 968, 1149
SaqAI TTAA 2 cut(s) 689, 953
SatI GCNGC 3 cut(s) 8, 1012, 1316
Sau3AI GATC 5 cut(s) 182, 217, 834, 1120, 1325
Sau96I GGNCC 3 cut(s) 257, 302, 770
SbfI CCTGCAGG 1 cut(s) 391
ScaI AGTACT 1 cut(s) 671
SchI GAGTC 3 cut(s) 389, 999, 1221
ScrFI CCNGG 2 cut(s) 117, 261
SdaI CCTGCAGG 1 cut(s) 391
SfaNI GCATC 3 cut(s) 688, 781, 1050
SfcI CTRYAG 1 cut(s) 387
Sfr274I CTCGAG 1 cut(s) 467
SfuI TTCGAA 2 cut(s) 636, 708
SinI GGWCC 3 cut(s) 257, 302, 770
SlaI CTCGAG 1 cut(s) 467
SmlI CTYRAG 3 cut(s) 332, 467, 885
SmoI CTYRAG 3 cut(s) 332, 467, 885
Sse8387I CCTGCAGG 1 cut(s) 391
SsiI CCGC 1 cut(s) 318
StyD4I CCNGG 2 cut(s) 115, 259
TaaI ACNGT 5 cut(s) 55, 163, 943, 1115, 1259
TaiI ACGT 1 cut(s) 852
TaqI TCGA 8 cut(s) 13, 468, 489, 636, 708, 837, 858, 930
TatI WGTACW 3 cut(s) 669, 967, 1148
TfiI GAWTC 1 cut(s) 1127
Tru1I TTAA 2 cut(s) 689, 953
Tru9I TTAA 2 cut(s) 689, 953
TscAI CASTG 4 cut(s) 618, 1120, 1164, 1264
TseFI GTSAC 3 cut(s) 381, 991, 1259
TseI GCWGC 3 cut(s) 7, 1011, 1315
Tsp45I GTSAC 3 cut(s) 381, 991, 1259
TspDTI ATGAA 8 cut(s) 183, 258, 936, 951, 971, 1055, 1314, 1335
TspGWI ACGGA 4 cut(s) 315, 865, 1168, 1182
TspRI CASTG 4 cut(s) 618, 1120, 1164, 1264
VpaK11BI GGWCC 3 cut(s) 257, 302, 770
VspI ATTAAT 1 cut(s) 689
XagI CCTNNNNNAGG 1 cut(s) 15
XapI RAATTY 5 cut(s) 494, 638, 712, 741, 1095
XceI RCATGY 1 cut(s) 149
XhoI CTCGAG 1 cut(s) 467
XmiI GTMKAC 1 cut(s) 1254
ZrmI AGTACT 1 cut(s) 671
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.