pycom08g20650

Belongs to the peptidase S10 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0001027
Physical Location & Seq
Forward (+)
20367191 .. 20368085
895 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g20650.2

Sequence Viewer

Length: 570 bp
ATGCTATACTTGGAGTCACCTGCAGGAGTAGGCTTCTCCTACTCAGCAAACAAATCATTCTATGGCTTCGTGAACGACGATATCACAGGTTTTCCACCAGATAATCTCGAGTTCCTTCGGCACTGGTTTGACGAATTCCCTGAGTATAAAAATAGGGATTTGTTTATCACAGGAGAGAGCTATGCAGGCCATTATGTTCCACTGCTTGCACAACTCATTATCCAGTCAAAACTGAAGTTCAATCTGAAAGCAGTAGCAGTAAGCAAACGAGGATCGAAAAACGGATCTCATGGATTAATATCAGATGCGACCTTCGAAAATTTGACTTCAGTCTGCAACTATTCTGAAATTTATAGACAGGAGGTAATTCGTGGTGGTCCACTTACCCCTACTTGTGATGCAGTAGCGAAACAATTTGAAAGAGAAGCCCCTAGAAATTTGATCAACCGATATGATGTTACTCTCGATGTCTGTTTATCATCGGTCGAAGCTCAATCTTTCCAACTGGTAAGCCTTGGAAAAGGGGGCATGTCAAAGCCTATAAATAGACTACCAAGTCAAGCATTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_S10

Protein Analysis

190

Amino Acids

21.12

Weight (kDa)

6.3

Isoelectric Point (pI)

44.42

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 28
AasI GACNNNNNNGTC 1 cut(s) 555
Acc36I ACCTGC 1 cut(s) 28
AclWI GGATC 2 cut(s) 280, 292
AcsI RAATTY 4 cut(s) 134, 319, 348, 436
AcuI CTGAAG 2 cut(s) 254, 312
AgsI TTSAA 2 cut(s) 241, 419
AluBI AGCT 2 cut(s) 180, 491
AluI AGCT 2 cut(s) 180, 491
AlwI GGATC 2 cut(s) 280, 292
Ama87I CYCGRG 1 cut(s) 107
AoxI GGCC 1 cut(s) 187
ApoI RAATTY 4 cut(s) 134, 319, 348, 436
AseI ATTAAT 1 cut(s) 296
AspS9I GGNCC 1 cut(s) 377
AsuHPI GGTGA 1 cut(s) 9
AsuII TTCGAA 1 cut(s) 315
AvaI CYCGRG 1 cut(s) 107
AvaII GGWCC 1 cut(s) 377
BclI TGATCA 1 cut(s) 441
BfaI CTAG 1 cut(s) 432
BfmI CTRYAG 1 cut(s) 21
BfuAI ACCTGC 1 cut(s) 28
Bme18I GGWCC 1 cut(s) 377
BmeT110I CYCGRG 1 cut(s) 107
BmgT120I GGNCC 1 cut(s) 377
BmsI GCATC 2 cut(s) 295, 388
BoxI GACNNNNGTC 1 cut(s) 329
Bpu14I TTCGAA 1 cut(s) 315
BsaBI GATNNNNATC 1 cut(s) 298
BsaJI CCNNGG 1 cut(s) 514
Bse1I ACTGG 3 cut(s) 128, 223, 510
Bse8I GATNNNNATC 1 cut(s) 298
BseDI CCNNGG 1 cut(s) 514
BseJI GATNNNNATC 1 cut(s) 298
BseMII CTCAG 2 cut(s) 57, 132
BseNI ACTGG 3 cut(s) 128, 223, 510
Bsh1285I CGRYCG 1 cut(s) 486
BshFI GGCC 1 cut(s) 189
BsiEI CGRYCG 1 cut(s) 486
BsiHKCI CYCGRG 1 cut(s) 107
BsnI GGCC 1 cut(s) 189
BsoBI CYCGRG 1 cut(s) 107
Bsp119I TTCGAA 1 cut(s) 315
Bsp143I GATC 3 cut(s) 272, 284, 441
BspANI GGCC 1 cut(s) 189
BspCNI CTCAG 2 cut(s) 56, 133
BspMAI CTGCAG 1 cut(s) 25
BspMI ACCTGC 1 cut(s) 28
BspPI GGATC 2 cut(s) 280, 292
BspT104I TTCGAA 1 cut(s) 315
BsrI ACTGG 3 cut(s) 128, 223, 510
BssECI CCNNGG 1 cut(s) 514
BssMI GATC 3 cut(s) 272, 284, 441
BssT1I CCWWGG 1 cut(s) 514
BstBI TTCGAA 1 cut(s) 315
BstC8I GCNNGC 2 cut(s) 187, 207
BstDEI CTNAG 2 cut(s) 43, 141
BstKTI GATC 3 cut(s) 275, 287, 444
BstMBI GATC 3 cut(s) 272, 284, 441
BstMCI CGRYCG 1 cut(s) 486
BstMWI GCNNNNNNNGC 1 cut(s) 186
BstNSI RCATGY 1 cut(s) 532
BstPAI GACNNNNGTC 1 cut(s) 329
BstSFI CTRYAG 1 cut(s) 21
BstX2I RGATCY 1 cut(s) 284
BstYI RGATCY 1 cut(s) 284
BsuRI GGCC 1 cut(s) 189
BtsI GCAGTG 1 cut(s) 200
BtsIMutI CAGTG 2 cut(s) 121, 200
BveI ACCTGC 1 cut(s) 28
Cac8I GCNNGC 2 cut(s) 187, 207
Cfr13I GGNCC 1 cut(s) 377
CviAII CATG 2 cut(s) 290, 529
CviJI RGCY 8 cut(s) 33, 66, 180, 189, 428, 491, 513, 538
CviKI_1 RGCY 8 cut(s) 33, 66, 180, 189, 428, 491, 513, 538
DdeI CTNAG 2 cut(s) 43, 141
DpnI GATC 3 cut(s) 274, 286, 443
DpnII GATC 3 cut(s) 272, 284, 441
DrdI GACNNNNNNGTC 1 cut(s) 555
DseDI GACNNNNNNGTC 1 cut(s) 555
Eco130I CCWWGG 1 cut(s) 514
Eco32I GATATC 1 cut(s) 82
Eco47I GGWCC 1 cut(s) 377
Eco57I CTGAAG 2 cut(s) 254, 312
Eco88I CYCGRG 1 cut(s) 107
EcoRI GAATTC 1 cut(s) 134
EcoRV GATATC 1 cut(s) 82
EcoT14I CCWWGG 1 cut(s) 514
ErhI CCWWGG 1 cut(s) 514
FaeI CATG 2 cut(s) 293, 532
FatI CATG 2 cut(s) 289, 528
FbaI TGATCA 1 cut(s) 441
FspBI CTAG 1 cut(s) 432
HaeIII GGCC 1 cut(s) 189
Hin1II CATG 2 cut(s) 293, 532
HinfI GANTC 1 cut(s) 14
HphI GGTGA 1 cut(s) 9
Hpy166II GTNNAC 2 cut(s) 73, 380
Hpy188I TCNGA 3 cut(s) 246, 304, 346
Hpy188III TCNNGA 3 cut(s) 70, 107, 464
Hpy8I GTNNAC 2 cut(s) 73, 380
Hpy99I CGWCG 1 cut(s) 80
HpyAV CCTTC 2 cut(s) 125, 322
HpyCH4V TGCA 5 cut(s) 23, 185, 209, 336, 401
HpyF10VI GCNNNNNNNGC 1 cut(s) 186
HpyF3I CTNAG 2 cut(s) 43, 141
Hsp92II CATG 2 cut(s) 293, 532
Ksp22I TGATCA 1 cut(s) 441
Kzo9I GATC 3 cut(s) 272, 284, 441
LweI GCATC 2 cut(s) 295, 388
MaeI CTAG 1 cut(s) 432
MaeIII GTNAC 2 cut(s) 15, 457
MalI GATC 3 cut(s) 274, 286, 443
MboI GATC 3 cut(s) 272, 284, 441
MflI RGATCY 1 cut(s) 284
MluCI AATT 6 cut(s) 134, 319, 348, 366, 413, 436
MlyI GAGTC 1 cut(s) 23
MmeI TCCRAC 1 cut(s) 526
MnlI CCTC 2 cut(s) 263, 355
MseI TTAA 1 cut(s) 296
MwoI GCNNNNNNNGC 1 cut(s) 186
NdeII GATC 3 cut(s) 272, 284, 441
NlaIII CATG 2 cut(s) 293, 532
NmuCI GTSAC 1 cut(s) 15
NspI RCATGY 1 cut(s) 532
NspV TTCGAA 1 cut(s) 315
PaeR7I CTCGAG 1 cut(s) 107
PaqCI CACCTGC 1 cut(s) 28
PcsI WCGNNNNNNNCGW 1 cut(s) 75
PleI GAGTC 1 cut(s) 22
PpsI GAGTC 1 cut(s) 22
PshAI GACNNNNGTC 1 cut(s) 329
PshBI ATTAAT 1 cut(s) 296
PspPI GGNCC 1 cut(s) 377
PstI CTGCAG 1 cut(s) 25
PsuI RGATCY 1 cut(s) 284
SaqAI TTAA 1 cut(s) 296
Sau3AI GATC 3 cut(s) 272, 284, 441
Sau96I GGNCC 1 cut(s) 377
SbfI CCTGCAGG 1 cut(s) 25
SchI GAGTC 1 cut(s) 23
SdaI CCTGCAGG 1 cut(s) 25
SetI ASST 6 cut(s) 22, 91, 182, 314, 366, 493
SfaNI GCATC 2 cut(s) 295, 388
SfcI CTRYAG 1 cut(s) 21
Sfr274I CTCGAG 1 cut(s) 107
SfuI TTCGAA 1 cut(s) 315
SinI GGWCC 1 cut(s) 377
SlaI CTCGAG 1 cut(s) 107
SmlI CTYRAG 1 cut(s) 107
SmoI CTYRAG 1 cut(s) 107
Sse8387I CCTGCAGG 1 cut(s) 25
Sse9I AATT 6 cut(s) 134, 319, 348, 366, 413, 436
SspMI CTAG 1 cut(s) 432
StyI CCWWGG 1 cut(s) 514
TaqI TCGA 5 cut(s) 108, 275, 315, 465, 486
TaqII GACCGA 1 cut(s) 472
TasI AATT 6 cut(s) 134, 319, 348, 366, 413, 436
Tru1I TTAA 1 cut(s) 296
Tru9I TTAA 1 cut(s) 296
TscAI CASTG 2 cut(s) 128, 207
TseFI GTSAC 1 cut(s) 15
Tsp45I GTSAC 1 cut(s) 15
TspGWI ACGGA 1 cut(s) 297
TspRI CASTG 2 cut(s) 128, 207
VpaK11BI GGWCC 1 cut(s) 377
VspI ATTAAT 1 cut(s) 296
XapI RAATTY 4 cut(s) 134, 319, 348, 436
XceI RCATGY 1 cut(s) 532
XhoI CTCGAG 1 cut(s) 107
XspI CTAG 1 cut(s) 432
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.