pycom08g20640

Belongs to the peptidase S10 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0001027
Physical Location & Seq
Forward (+)
20356806 .. 20365275
8470 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g20640.6

Sequence Viewer

Length: 1263 bp
ATGCATAACACTCGCTTAGAATCTCAAAGTAGAGTACCTTTCTTGCAAGACTCTCTCTCTCTCTCACTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCATGATGCAGGCTCAACGAGGGAGTATCGTCGTCGTCGCCACAGTCTTTGCAACTGTCATCCAAGCGTTTTCCACGGCAAACTCCCTCCAAGAAGCTGATAAAATCACAAGGTTGCCTGGTCAACCACAGGTCAACTTCCAGCAATATGCAGGATATGTAACTGTGGATGAAAAGCAAGGGAGATCACTTTTTTACTACTTTGTTGAAGCACAAACTGATCCTGCTTCAAAGCCTCTTGTTCTTTGGCTCAATGGAGGTCCTGGTTGTTCATCTGTTGGAGGAGCTTTCATCGAGCACGGACCTTTTCAACCAAGCGGAGACATTTTACTCAAGAAAGATTTTAGTTGGAATAGAGAAGCAAATATGCTATACTTGGAGTCACCTGCAGGAGTAGGCTTCTCCTACTCAGCAAACAAATCATTCTATGGCTTCGTGAACGACGATATCACAGCACGAGATAATCTCGAGTTCCTTCAGCGCTGGTTCGACGAATTCCCTGAGTATAAAAATAGGGATTTGTTTATCACAGGAGTGAGCTATGCAGGACACTATGTTCCGCAGCTTGCACAACTCATTATCCAATCAAAACTGAAGTTCAATCTGAAAGCAGTAGCAATTGGCAATCTTCTTCTCGAATTCAATACTGATATCAACTCACGAGCAGATTACTGGTGGTCTCATGGATTAATATCAGATGCAACCTTCGCAAATTTGACTTCAGTCTGCAACTATTCTGAAATTTTTAGACAGGAGCGAATTCGTGGTGGTCCGCTTACCCCTGCTTGTGATGCAGTGGCGAACCAATTTGAAAGAGAAGTCCCTGAACATTTGATCAACCGATATGATGTTACTCTCGATGTCTGTTTATCATCGGTCAAAGCTTTCCAACTGCGAAAAGCAGTGAAGATAGATGTCTGTCTCGTAGATGAAATAGTTTCATACTTAAACCGACCAGATGTTCAGGAGGCGCTTCATGCCCGAATCACAAATTGGACATTTTGCAGCAACAGAGTTGTCATCCAGTATGACCTGAAAAACCTGGAAACACCGATGCTTCCAGTTTTAGGTGACCTTGTCAAGTCTGGAATTCGGGTTTTGATTTACAGTGGATCGATCAAGATTCTATTATTCCACTATCGAGTACACGGACACTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_S10

Protein Analysis

421

Amino Acids

47.34

Weight (kDa)

6.16

Isoelectric Point (pI)

39.62

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 496
Acc36I ACCTGC 1 cut(s) 496
AciI CCGC 3 cut(s) 420, 662, 875
AclWI GGATC 2 cut(s) 317, 1222
AcsI RAATTY 6 cut(s) 596, 740, 814, 843, 861, 1191
AcuI CTGAAG 3 cut(s) 563, 716, 807
AfaI GTAC 2 cut(s) 36, 1248
AfeI AGCGCT 1 cut(s) 584
AfiI CCNNNNNNNGG 1 cut(s) 1169
AgsI TTSAA 6 cut(s) 311, 333, 413, 703, 745, 914
AjnI CCWGG 3 cut(s) 220, 364, 1143
AjuI GAANNNNNNNTTGG 2 cut(s) 1078, 1110
AleI CACNNNNGTG 1 cut(s) 635
AloI GAACNNNNNNTCC 2 cut(s) 642, 674
AluBI AGCT 5 cut(s) 200, 389, 642, 667, 986
AluI AGCT 5 cut(s) 200, 389, 642, 667, 986
Alw21I GWGCWC 1 cut(s) 402
Alw26I GTCTC 3 cut(s) 417, 786, 1028
AlwI GGATC 2 cut(s) 317, 1222
Ama87I CYCGRG 1 cut(s) 569
Aor51HI AGCGCT 1 cut(s) 584
ApeKI GCWGC 2 cut(s) 664, 1107
ApoI RAATTY 6 cut(s) 596, 740, 814, 843, 861, 1191
AseI ATTAAT 1 cut(s) 791
AspLEI GCGC 2 cut(s) 585, 1075
AspS9I GGNCC 3 cut(s) 362, 404, 872
AsuHPI GGTGA 2 cut(s) 477, 1184
AvaI CYCGRG 1 cut(s) 569
AvaII GGWCC 3 cut(s) 362, 404, 872
BauI CACGAG 2 cut(s) 558, 762
Bbv12I GWGCWC 1 cut(s) 402
BbvI GCAGC 2 cut(s) 676, 1119
BceAI ACGGC 1 cut(s) 195
BcgI CGANNNNNNTGC 1 cut(s) 27
BciT130I CCWGG 3 cut(s) 222, 366, 1145
BclI TGATCA 1 cut(s) 936
BcoDI GTCTC 3 cut(s) 417, 786, 1028
BfmI CTRYAG 1 cut(s) 489
BfoI RGCGCY 2 cut(s) 586, 1076
BfuAI ACCTGC 1 cut(s) 496
BisI GCNGC 2 cut(s) 665, 1108
BlsI GCNGC 2 cut(s) 666, 1109
Bme1390I CCNGG 3 cut(s) 222, 366, 1145
Bme18I GGWCC 3 cut(s) 362, 404, 872
BmeT110I CYCGRG 1 cut(s) 569
BmgT120I GGNCC 3 cut(s) 362, 404, 872
BmrFI CCNGG 3 cut(s) 222, 366, 1145
BmsI GCATC 4 cut(s) 99, 790, 883, 1146
BoxI GACNNNNGTC 1 cut(s) 824
BplI GAGNNNNNCTC 2 cut(s) 552, 584
BpuEI CTTGAG 1 cut(s) 419
Bsa29I ATCGAT 1 cut(s) 1217
BsaBI GATNNNNATC 1 cut(s) 793
BsaI GGTCTC 1 cut(s) 786
BsaJI CCNNGG 1 cut(s) 177
Bsc4I CCNNNNNNNGG 1 cut(s) 1169
Bse1I ACTGG 4 cut(s) 779, 1126, 1163, 1262
Bse8I GATNNNNATC 1 cut(s) 793
BseBI CCWGG 3 cut(s) 222, 366, 1145
BseCI ATCGAT 1 cut(s) 1217
BseDI CCNNGG 1 cut(s) 177
BseGI GGATG 3 cut(s) 162, 277, 1122
BseJI GATNNNNATC 1 cut(s) 793
BseLI CCNNNNNNNGG 1 cut(s) 1169
BseMII CTCAG 2 cut(s) 525, 594
BseNI ACTGG 4 cut(s) 779, 1126, 1163, 1262
BseRI GAGGAG 1 cut(s) 399
BseXI GCAGC 2 cut(s) 676, 1119
BshVI ATCGAT 1 cut(s) 1217
BsiHKAI GWGCWC 1 cut(s) 402
BsiHKCI CYCGRG 1 cut(s) 569
BslFI GGGAC 1 cut(s) 908
BslI CCNNNNNNNGG 1 cut(s) 1169
BsmAI GTCTC 3 cut(s) 417, 786, 1028
BsmFI GGGAC 1 cut(s) 908
Bso31I GGTCTC 1 cut(s) 786
BsoBI CYCGRG 1 cut(s) 569
Bsp1286I GDGCHC 1 cut(s) 402
Bsp143I GATC 5 cut(s) 287, 322, 936, 1214, 1218
BspACI CCGC 3 cut(s) 420, 662, 875
BspCNI CTCAG 2 cut(s) 524, 595
BspDI ATCGAT 1 cut(s) 1217
BspHI TCATGA 1 cut(s) 105
BspMAI CTGCAG 1 cut(s) 493
BspMI ACCTGC 1 cut(s) 496
BspPI GGATC 2 cut(s) 317, 1222
BspTNI GGTCTC 1 cut(s) 786
BsrI ACTGG 4 cut(s) 779, 1126, 1163, 1262
BssECI CCNNGG 1 cut(s) 177
BssMI GATC 5 cut(s) 287, 322, 936, 1214, 1218
BssSI CACGAG 2 cut(s) 558, 762
Bst2BI CACGAG 2 cut(s) 558, 762
Bst2UI CCWGG 3 cut(s) 222, 366, 1145
Bst4CI ACNGT 4 cut(s) 148, 160, 268, 1211
BstC8I GCNNGC 2 cut(s) 114, 669
BstDEI CTNAG 3 cut(s) 16, 511, 603
BstDSI CCRYGG 1 cut(s) 177
BstEII GGTNACC 1 cut(s) 1172
BstF5I GGATG 3 cut(s) 162, 277, 1122
BstH2I RGCGCY 2 cut(s) 586, 1076
BstHHI GCGC 2 cut(s) 585, 1075
BstKTI GATC 5 cut(s) 290, 325, 939, 1217, 1221
BstMAI GTCTC 3 cut(s) 417, 786, 1028
BstMBI GATC 5 cut(s) 287, 322, 936, 1214, 1218
BstMWI GCNNNNNNNGC 3 cut(s) 809, 893, 1079
BstNI CCWGG 3 cut(s) 222, 366, 1145
BstPAI GACNNNNGTC 1 cut(s) 824
BstPI GGTNACC 1 cut(s) 1172
BstSCI CCNGG 3 cut(s) 220, 364, 1143
BstSFI CTRYAG 1 cut(s) 489
BstV1I GCAGC 2 cut(s) 676, 1119
Bsu15I ATCGAT 1 cut(s) 1217
BsuTUI ATCGAT 1 cut(s) 1217
BtgI CCRYGG 1 cut(s) 177
BtsCI GGATG 3 cut(s) 162, 277, 1122
BtsI GCAGTG 2 cut(s) 903, 1011
BtsIMutI CAGTG 4 cut(s) 903, 1011, 1216, 1255
BveI ACCTGC 1 cut(s) 496
Cac8I GCNNGC 2 cut(s) 114, 669
CciI TCATGA 1 cut(s) 105
CfoI GCGC 2 cut(s) 585, 1075
Cfr13I GGNCC 3 cut(s) 362, 404, 872
ClaI ATCGAT 1 cut(s) 1217
Csp6I GTAC 2 cut(s) 35, 1247
CspCI CAANNNNNGTGG 2 cut(s) 133, 168
CviAII CATG 3 cut(s) 106, 785, 1079
CviQI GTAC 2 cut(s) 35, 1247
DdeI CTNAG 3 cut(s) 16, 511, 603
DpnI GATC 5 cut(s) 289, 324, 938, 1216, 1220
DpnII GATC 5 cut(s) 287, 322, 936, 1214, 1218
Eco31I GGTCTC 1 cut(s) 786
Eco32I GATATC 2 cut(s) 550, 754
Eco47I GGWCC 3 cut(s) 362, 404, 872
Eco47III AGCGCT 1 cut(s) 584
Eco57I CTGAAG 3 cut(s) 563, 716, 807
Eco88I CYCGRG 1 cut(s) 569
Eco91I GGTNACC 1 cut(s) 1172
EcoO109I RGGNCCY 1 cut(s) 362
EcoO65I GGTNACC 1 cut(s) 1172
EcoRI GAATTC 4 cut(s) 596, 740, 861, 1191
EcoRII CCWGG 3 cut(s) 220, 364, 1143
EcoRV GATATC 2 cut(s) 550, 754
EcoT22I ATGCAT 1 cut(s) 6
FaeI CATG 3 cut(s) 109, 788, 1082
FaqI GGGAC 1 cut(s) 908
FatI CATG 3 cut(s) 105, 784, 1078
FbaI TGATCA 1 cut(s) 936
Fnu4HI GCNGC 2 cut(s) 665, 1108
FokI GGATG 3 cut(s) 149, 284, 1109
Fsp4HI GCNGC 2 cut(s) 665, 1108
GlaI GCGC 2 cut(s) 584, 1074
GluI GCNGC 2 cut(s) 665, 1108
HaeII RGCGCY 2 cut(s) 586, 1076
HhaI GCGC 2 cut(s) 585, 1075
Hin1II CATG 3 cut(s) 109, 788, 1082
Hin6I GCGC 2 cut(s) 583, 1073
HinP1I GCGC 2 cut(s) 583, 1073
HincII GTYRAC 2 cut(s) 227, 238
HindII GTYRAC 2 cut(s) 227, 238
HindIII AAGCTT 1 cut(s) 984
HinfI GANTC 5 cut(s) 20, 50, 482, 1086, 1225
HphI GGTGA 2 cut(s) 477, 1184
Hpy166II GTNNAC 4 cut(s) 227, 238, 541, 1249
Hpy188I TCNGA 3 cut(s) 708, 799, 841
Hpy8I GTNNAC 4 cut(s) 227, 238, 541, 1249
Hpy99I CGWCG 5 cut(s) 137, 140, 143, 548, 596
HpyAV CCTTC 2 cut(s) 587, 817
HpyCH4III ACNGT 4 cut(s) 148, 160, 268, 1211
HpyF10VI GCNNNNNNNGC 3 cut(s) 809, 893, 1079
HpyF3I CTNAG 3 cut(s) 16, 511, 603
Hsp92II CATG 3 cut(s) 109, 788, 1082
HspAI GCGC 2 cut(s) 583, 1073
Ksp22I TGATCA 1 cut(s) 936
Kzo9I GATC 5 cut(s) 287, 322, 936, 1214, 1218
LmnI GCTCC 2 cut(s) 386, 856
Lsp1109I GCAGC 2 cut(s) 676, 1119
LweI GCATC 4 cut(s) 99, 790, 883, 1146
MaeIII GTNAC 4 cut(s) 262, 483, 952, 1172
MalI GATC 5 cut(s) 289, 324, 938, 1216, 1220
MboI GATC 5 cut(s) 287, 322, 936, 1214, 1218
MboII GAAGA 3 cut(s) 722, 725, 1021
MfeI CAATTG 1 cut(s) 720
MhlI GDGCHC 1 cut(s) 402
MluCI AATT 9 cut(s) 596, 720, 740, 814, 843, 861, 908, 1093, 1191
MlyI GAGTC 2 cut(s) 44, 491
MmeI TCCRAC 3 cut(s) 361, 431, 1015
MnlI CCTC 6 cut(s) 116, 200, 348, 353, 377, 1063
Mph1103I ATGCAT 1 cut(s) 6
MseI TTAA 2 cut(s) 791, 1049
MslI CAYNNNNRTG 1 cut(s) 635
MspR9I CCNGG 3 cut(s) 222, 366, 1145
MunI CAATTG 1 cut(s) 720
MvaI CCWGG 3 cut(s) 222, 366, 1145
MwoI GCNNNNNNNGC 3 cut(s) 809, 893, 1079
NdeII GATC 5 cut(s) 287, 322, 936, 1214, 1218
NlaIII CATG 3 cut(s) 109, 788, 1082
NmuCI GTSAC 2 cut(s) 483, 1172
NsiI ATGCAT 1 cut(s) 6
OliI CACNNNNGTG 1 cut(s) 635
PaeR7I CTCGAG 1 cut(s) 569
PagI TCATGA 1 cut(s) 105
PaqCI CACCTGC 1 cut(s) 496
PcsI WCGNNNNNNNCGW 1 cut(s) 543
PfeI GAWTC 3 cut(s) 20, 1086, 1225
PflFI GACNNNGTC 1 cut(s) 1178
PkrI GCNGC 2 cut(s) 666, 1109
PleI GAGTC 2 cut(s) 44, 490
PpsI GAGTC 2 cut(s) 44, 490
PpuMI RGGWCCY 1 cut(s) 362
PshAI GACNNNNGTC 1 cut(s) 824
PshBI ATTAAT 1 cut(s) 791
Psp5II RGGWCCY 1 cut(s) 362
Psp6I CCWGG 3 cut(s) 220, 364, 1143
PspEI GGTNACC 1 cut(s) 1172
PspGI CCWGG 3 cut(s) 220, 364, 1143
PspPI GGNCC 3 cut(s) 362, 404, 872
PspPPI RGGWCCY 1 cut(s) 362
PstI CTGCAG 1 cut(s) 493
PsyI GACNNNGTC 1 cut(s) 1178
RsaI GTAC 2 cut(s) 36, 1248
RsaNI GTAC 2 cut(s) 35, 1247
RseI CAYNNNNRTG 1 cut(s) 635
SaqAI TTAA 2 cut(s) 791, 1049
SatI GCNGC 2 cut(s) 665, 1108
Sau3AI GATC 5 cut(s) 287, 322, 936, 1214, 1218
Sau96I GGNCC 3 cut(s) 362, 404, 872
SbfI CCTGCAGG 1 cut(s) 493
SchI GAGTC 2 cut(s) 44, 491
ScrFI CCNGG 3 cut(s) 222, 366, 1145
SdaI CCTGCAGG 1 cut(s) 493
SduI GDGCHC 1 cut(s) 402
SfaNI GCATC 4 cut(s) 99, 790, 883, 1146
SfcI CTRYAG 1 cut(s) 489
Sfr274I CTCGAG 1 cut(s) 569
SinI GGWCC 3 cut(s) 362, 404, 872
SlaI CTCGAG 1 cut(s) 569
SmiMI CAYNNNNRTG 1 cut(s) 635
SmlI CTYRAG 2 cut(s) 434, 569
SmoI CTYRAG 2 cut(s) 434, 569
Sse8387I CCTGCAGG 1 cut(s) 493
Sse9I AATT 9 cut(s) 596, 720, 740, 814, 843, 861, 908, 1093, 1191
SsiI CCGC 3 cut(s) 420, 662, 875
StyD4I CCNGG 3 cut(s) 220, 364, 1143
TaaI ACNGT 4 cut(s) 148, 160, 268, 1211
TaqI TCGA 7 cut(s) 396, 570, 591, 738, 960, 1217, 1243
TaqII GACCGA 1 cut(s) 967
TasI AATT 9 cut(s) 596, 720, 740, 814, 843, 861, 908, 1093, 1191
TatI WGTACW 1 cut(s) 1246
TfiI GAWTC 3 cut(s) 20, 1086, 1225
Tru1I TTAA 2 cut(s) 791, 1049
Tru9I TTAA 2 cut(s) 791, 1049
TscAI CASTG 4 cut(s) 903, 1011, 1216, 1262
TseFI GTSAC 2 cut(s) 483, 1172
TseI GCWGC 2 cut(s) 664, 1107
Tsp45I GTSAC 2 cut(s) 483, 1172
TspDTI ATGAA 6 cut(s) 288, 363, 382, 1032, 1047, 1067
TspGWI ACGGA 1 cut(s) 417
TspRI CASTG 4 cut(s) 903, 1011, 1216, 1262
Tth111I GACNNNGTC 1 cut(s) 1178
VpaK11BI GGWCC 3 cut(s) 362, 404, 872
VspI ATTAAT 1 cut(s) 791
XapI RAATTY 6 cut(s) 596, 740, 814, 843, 861, 1191
XhoI CTCGAG 1 cut(s) 569
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.