MD08G1238200.v1.1

Belongs to the peptidase S10 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0001027
Physical Location & Seq
Forward (+)
30518095 .. 30530264
12170 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1238200.v1.1.491

Sequence Viewer

Length: 1638 bp
ATGATGCAGCCTCAACGAGGGAGTATCGTCGTCGTCGCCGCAGTCTTTGCAACTGTCATCCAAGCGTTTTCCACGGAAAACTCCCTCCAAGAAGCTGATAAAATCAAAAGGTTGCCAGGTCAACCACAAGTCAACTTCCAGCAATATGCAGGATATGTAACTGTGGATGAAAAGCAAGGGAGATCACTTTTTTACTACTTTGTTGAAGCACAAACTGATCCTGCTTCAAAGCCTCTTGTTCTTTGGCTCAATGGAGGTCCTGGTTGTTCATCTGTTGGAGGAGCTTTCATCGCGCACGGACCTTTTCAACCAAGCAGAGACATTTTACTCAAGAACGATTTTAGTTGGAATACAGAAGCAAATATGCTATACTTGGAGTCACCTGCAGGGGTAGGCTTCTCCTACTCAGCAAACAAATCATTCTATGGCTTCGTGAACGACGATATCACAGCACGAGATAATCTCAAGTTCCTTCGGCGCTGGTTCCACAAATTCCCTGAGTATAAAAATAGGGATTTGTTTATCACAGGACAGAGCTATGCAGGCCACTATGTTCCACAACTTGCACAACTCATTATCCAATCAAAACTGAAGTTCAATCTGAAGGCGGTAGCAATTGGCAATCCTCTTTTGGAATTCAATACTGATGCCAACTCACCAGCAGAGTACTGGTGGTCTCATGGATTAATATCAGATGCGACCTTCGAAAATTTGACTTCAGTCTGCAACTCTTCTGAAATTTTTAGACAGGAGGTAATTCGTGGTGGTCCACTTAGCCCTGCTTGTGATGCAGTAGCGAACCAATTTGAAAGAGAAGTCCCTGGACATTTGATCAACCAATATGACGTTACTCTCGATGTCTGTTTATCATCCGTCGAAGCTCAATCTTTCCAACTGCGAAAAACAGTGAAGATAGATGTCTGTATCGGAGATGAAACTGTTACATACTTCAACCGACCAGATGTACAGGAGGCACTTCATGCCCGAGTCACAAATTGGACAATTTGCAGCAACGGAGGTGTCCTTGAGTATGACATGAAAAACCATGAAATACCGATGCTTCCAGTTTTAGGTGACCTTGTCAAGTCTGGAATTCGGGTTTTGATTTACAGTGGTGATCAAGATTCTATTATTCCACTAACGAGTACACGGACACTGGTGAACGGATTGGCAAGGGACTTGGGGCTACAAACGAACGTGCCTTACAGAGTCTGGGTTCAAGAAAAACAGGTTGCTGGATGGACACAAGTATACAGTGACGCTTTATCTTACGCTACCATAAGAGGAGCAGGCCATGAAGCTCCAATTTCGCAGCCAAAGAGATCACTACTTCTGTTTACCTCATTTCTGAGAGGAAAGCCACTGCCAAAAGCCTTAAATCAAGATTCTATTATTCCACTAACGAGTACACGGACACTGGTGAACGGATTGGCAGAGGACTTGGGGCTACAAACGAATGTGCCTTACAGAGTCTGGTTTGAAGAAAAACAGGTTGTTGGACGGACACAAGTATACAGTGATGCTTTATCTTACGCTACCATAAGAGGAGCAGGCCATAAAGCTCCAATTTCGCAGCCAGATAGATCATTGCTTCTATTTACTTCATTTCTGACAGGAATGCCATTGCCAAAAGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_S10

Protein Analysis

546

Amino Acids

60.6

Weight (kDa)

6.53

Isoelectric Point (pI)

40.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S10 PF00450 39 - 452 1.2e-123 Serine carboxypeptidase
Peptidase_S10 PF00450 461 - 538 3e-11 Serine carboxypeptidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 391
Acc36I ACCTGC 1 cut(s) 391
AccI GTMKAC 2 cut(s) 1251, 1512
AccII CGCG 1 cut(s) 293
AciI CCGC 2 cut(s) 39, 608
AclWI GGATC 1 cut(s) 212
AcsI RAATTY 5 cut(s) 491, 635, 709, 738, 1092
AcuI CTGAAG 3 cut(s) 611, 623, 702
AfaI GTAC 4 cut(s) 668, 966, 1147, 1408
AfiI CCNNNNNNNGG 2 cut(s) 17, 1070
AgsI TTSAA 9 cut(s) 206, 228, 308, 598, 640, 809, 952, 1220, 1481
AjnI CCWGG 3 cut(s) 115, 259, 820
AluBI AGCT 6 cut(s) 95, 284, 537, 881, 1301, 1562
AluI AGCT 6 cut(s) 95, 284, 537, 881, 1301, 1562
Alw26I GTCTC 2 cut(s) 312, 681
AlwI GGATC 1 cut(s) 212
AlwNI CAGNNNCTG 2 cut(s) 1212, 1473
Ama87I CYCGRG 1 cut(s) 984
AoxI GGCC 3 cut(s) 544, 1291, 1552
ApeKI GCWGC 4 cut(s) 7, 1008, 1312, 1573
ApoI RAATTY 5 cut(s) 491, 635, 709, 738, 1092
AseI ATTAAT 1 cut(s) 686
AspLEI GCGC 2 cut(s) 295, 480
AspS9I GGNCC 3 cut(s) 257, 299, 767
AsuHPI GGTGA 6 cut(s) 372, 648, 1085, 1127, 1171, 1432
AsuII TTCGAA 1 cut(s) 705
AvaI CYCGRG 1 cut(s) 984
AvaII GGWCC 3 cut(s) 257, 299, 767
BauI CACGAG 1 cut(s) 453
BbvI GCAGC 4 cut(s) 19, 1020, 1324, 1585
BccI CCATC 1 cut(s) 1233
BciT130I CCWGG 3 cut(s) 117, 261, 822
BclI TGATCA 2 cut(s) 831, 1117
BcoDI GTCTC 2 cut(s) 312, 681
BfmI CTRYAG 1 cut(s) 384
BfoI RGCGCY 1 cut(s) 481
BfuAI ACCTGC 1 cut(s) 391
BisI GCNGC 5 cut(s) 8, 39, 1009, 1313, 1574
BlsI GCNGC 5 cut(s) 9, 40, 1010, 1314, 1575
BmcAI AGTACT 1 cut(s) 668
Bme1390I CCNGG 3 cut(s) 117, 261, 822
Bme18I GGWCC 3 cut(s) 257, 299, 767
BmeT110I CYCGRG 1 cut(s) 984
BmgT120I GGNCC 3 cut(s) 257, 299, 767
BmiI GGNNCC 1 cut(s) 485
BmrFI CCNGG 3 cut(s) 117, 261, 822
BmsI GCATC 5 cut(s) 637, 685, 778, 1047, 1510
BoxI GACNNNNGTC 1 cut(s) 719
BplI GAGNNNNNCTC 2 cut(s) 447, 479
Bpu14I TTCGAA 1 cut(s) 705
BpuEI CTTGAG 3 cut(s) 314, 449, 1046
BsaBI GATNNNNATC 1 cut(s) 688
BsaI GGTCTC 1 cut(s) 681
BsaJI CCNNGG 2 cut(s) 72, 820
Bsc4I CCNNNNNNNGG 2 cut(s) 17, 1070
Bse1I ACTGG 4 cut(s) 674, 1064, 1161, 1422
Bse3DI GCAATG 2 cut(s) 1586, 1622
Bse8I GATNNNNATC 1 cut(s) 688
BseBI CCWGG 3 cut(s) 117, 261, 822
BseDI CCNNGG 2 cut(s) 72, 820
BseGI GGATG 4 cut(s) 57, 172, 869, 1244
BseJI GATNNNNATC 1 cut(s) 688
BseLI CCNNNNNNNGG 2 cut(s) 17, 1070
BseMI GCAATG 2 cut(s) 1586, 1622
BseMII CTCAG 3 cut(s) 420, 489, 1340
BseNI ACTGG 4 cut(s) 674, 1064, 1161, 1422
BseRI GAGGAG 3 cut(s) 294, 1299, 1560
BseXI GCAGC 4 cut(s) 19, 1020, 1324, 1585
Bsh1236I CGCG 1 cut(s) 293
BshFI GGCC 3 cut(s) 546, 1293, 1554
BsiHKCI CYCGRG 1 cut(s) 984
BslFI GGGAC 2 cut(s) 803, 1190
BslI CCNNNNNNNGG 2 cut(s) 17, 1070
BsmAI GTCTC 2 cut(s) 312, 681
BsmFI GGGAC 2 cut(s) 803, 1190
BsmI GAATGC 1 cut(s) 1623
BsnI GGCC 3 cut(s) 546, 1293, 1554
Bso31I GGTCTC 1 cut(s) 681
BsoBI CYCGRG 1 cut(s) 984
Bsp119I TTCGAA 1 cut(s) 705
Bsp1407I TGTACA 1 cut(s) 964
Bsp143I GATC 6 cut(s) 182, 217, 831, 1117, 1322, 1583
BspACI CCGC 2 cut(s) 39, 608
BspANI GGCC 3 cut(s) 546, 1293, 1554
BspCNI CTCAG 3 cut(s) 419, 490, 1341
BspFNI CGCG 1 cut(s) 293
BspLI GGNNCC 1 cut(s) 485
BspMAI CTGCAG 1 cut(s) 388
BspMI ACCTGC 1 cut(s) 391
BspPI GGATC 1 cut(s) 212
BspT104I TTCGAA 1 cut(s) 705
BspTNI GGTCTC 1 cut(s) 681
BsrDI GCAATG 2 cut(s) 1586, 1622
BsrGI TGTACA 1 cut(s) 964
BsrI ACTGG 4 cut(s) 674, 1064, 1161, 1422
BssECI CCNNGG 2 cut(s) 72, 820
BssMI GATC 6 cut(s) 182, 217, 831, 1117, 1322, 1583
BssNAI GTATAC 2 cut(s) 1252, 1513
BssSI CACGAG 1 cut(s) 453
Bst1107I GTATAC 2 cut(s) 1252, 1513
Bst2BI CACGAG 1 cut(s) 453
Bst2UI CCWGG 3 cut(s) 117, 261, 822
Bst4CI ACNGT 7 cut(s) 55, 163, 907, 940, 1112, 1256, 1517
Bst6I CTCTTC 1 cut(s) 736
BstAPI GCANNNNNTGC 2 cut(s) 47, 980
BstAUI TGTACA 1 cut(s) 964
BstBI TTCGAA 1 cut(s) 705
BstC8I GCNNGC 3 cut(s) 544, 1291, 1552
BstDEI CTNAG 4 cut(s) 406, 498, 773, 1349
BstDSI CCRYGG 1 cut(s) 72
BstEII GGTNACC 1 cut(s) 1073
BstENI CCTNNNNNAGG 1 cut(s) 15
BstF5I GGATG 4 cut(s) 57, 172, 869, 1244
BstFNI CGCG 1 cut(s) 293
BstH2I RGCGCY 1 cut(s) 481
BstHHI GCGC 2 cut(s) 295, 480
BstKTI GATC 6 cut(s) 185, 220, 834, 1120, 1325, 1586
BstMAI GTCTC 2 cut(s) 312, 681
BstMBI GATC 6 cut(s) 182, 217, 831, 1117, 1322, 1583
BstMWI GCNNNNNNNGC 5 cut(s) 47, 290, 543, 788, 980
BstNI CCWGG 3 cut(s) 117, 261, 822
BstPAI GACNNNNGTC 1 cut(s) 719
BstPI GGTNACC 1 cut(s) 1073
BstSCI CCNGG 3 cut(s) 115, 259, 820
BstSFI CTRYAG 1 cut(s) 384
BstUI CGCG 1 cut(s) 293
BstV1I GCAGC 4 cut(s) 19, 1020, 1324, 1585
BstZ17I GTATAC 2 cut(s) 1252, 1513
BsuRI GGCC 3 cut(s) 546, 1293, 1554
BtgI CCRYGG 1 cut(s) 72
BtgZI GCGATG 1 cut(s) 274
BtsCI GGATG 4 cut(s) 57, 172, 869, 1244
BtsI GCAGTG 1 cut(s) 1361
BtsIMutI CAGTG 7 cut(s) 912, 1117, 1154, 1261, 1361, 1415, 1522
BveI ACCTGC 1 cut(s) 391
Cac8I GCNNGC 3 cut(s) 544, 1291, 1552
CaiI CAGNNNCTG 2 cut(s) 1212, 1473
CfoI GCGC 2 cut(s) 295, 480
Cfr13I GGNCC 3 cut(s) 257, 299, 767
CseI GACGC 1 cut(s) 1268
Csp6I GTAC 4 cut(s) 667, 965, 1146, 1407
CviAII CATG 5 cut(s) 680, 980, 1036, 1046, 1295
CviQI GTAC 4 cut(s) 667, 965, 1146, 1407
DdeI CTNAG 4 cut(s) 406, 498, 773, 1349
DpnI GATC 6 cut(s) 184, 219, 833, 1119, 1324, 1585
DpnII GATC 6 cut(s) 182, 217, 831, 1117, 1322, 1583
Eam1104I CTCTTC 1 cut(s) 736
EarI CTCTTC 1 cut(s) 736
Eco31I GGTCTC 1 cut(s) 681
Eco32I GATATC 1 cut(s) 445
Eco47I GGWCC 3 cut(s) 257, 299, 767
Eco57I CTGAAG 3 cut(s) 611, 623, 702
Eco88I CYCGRG 1 cut(s) 984
Eco91I GGTNACC 1 cut(s) 1073
EcoNI CCTNNNNNAGG 1 cut(s) 15
EcoO109I RGGNCCY 1 cut(s) 257
EcoO65I GGTNACC 1 cut(s) 1073
EcoRI GAATTC 2 cut(s) 635, 1092
EcoRII CCWGG 3 cut(s) 115, 259, 820
EcoRV GATATC 1 cut(s) 445
FaeI CATG 5 cut(s) 683, 983, 1039, 1049, 1298
FaqI GGGAC 2 cut(s) 803, 1190
FatI CATG 5 cut(s) 679, 979, 1035, 1045, 1294
FbaI TGATCA 2 cut(s) 831, 1117
FblI GTMKAC 2 cut(s) 1251, 1512
Fnu4HI GCNGC 5 cut(s) 8, 39, 1009, 1313, 1574
FokI GGATG 4 cut(s) 44, 179, 856, 1251
Fsp4HI GCNGC 5 cut(s) 8, 39, 1009, 1313, 1574
GlaI GCGC 2 cut(s) 294, 479
GluI GCNGC 5 cut(s) 8, 39, 1009, 1313, 1574
HaeII RGCGCY 1 cut(s) 481
HaeIII GGCC 3 cut(s) 546, 1293, 1554
HgaI GACGC 1 cut(s) 1268
HhaI GCGC 2 cut(s) 295, 480
Hin1II CATG 5 cut(s) 683, 983, 1039, 1049, 1298
Hin6I GCGC 2 cut(s) 293, 478
HinP1I GCGC 2 cut(s) 293, 478
HincII GTYRAC 2 cut(s) 122, 133
HindII GTYRAC 2 cut(s) 122, 133
HinfI GANTC 6 cut(s) 377, 987, 1124, 1209, 1385, 1470
HphI GGTGA 6 cut(s) 372, 648, 1085, 1127, 1171, 1432
Hpy188I TCNGA 6 cut(s) 603, 694, 736, 929, 1350, 1611
Hpy188III TCNNGA 7 cut(s) 331, 433, 854, 1089, 1121, 1220, 1382
Hpy99I CGWCG 5 cut(s) 32, 35, 38, 443, 878
HpyAV CCTTC 3 cut(s) 482, 598, 712
HpyCH4III ACNGT 7 cut(s) 55, 163, 907, 940, 1112, 1256, 1517
HpyCH4IV ACGT 2 cut(s) 846, 1197
HpyCH4V TGCA 9 cut(s) 7, 50, 149, 386, 542, 566, 726, 791, 1008
HpyF10VI GCNNNNNNNGC 5 cut(s) 47, 290, 543, 788, 980
HpyF3I CTNAG 4 cut(s) 406, 498, 773, 1349
HpySE526I ACGT 2 cut(s) 846, 1197
Hsp92II CATG 5 cut(s) 683, 983, 1039, 1049, 1298
HspAI GCGC 2 cut(s) 293, 478
Ksp22I TGATCA 2 cut(s) 831, 1117
Kzo9I GATC 6 cut(s) 182, 217, 831, 1117, 1322, 1583
LmnI GCTCC 5 cut(s) 281, 1286, 1306, 1547, 1567
Lsp1109I GCAGC 4 cut(s) 19, 1020, 1324, 1585
LweI GCATC 5 cut(s) 637, 685, 778, 1047, 1510
MaeII ACGT 2 cut(s) 846, 1197
MaeIII GTNAC 7 cut(s) 157, 378, 847, 940, 988, 1073, 1256
MalI GATC 6 cut(s) 184, 219, 833, 1119, 1324, 1585
MboI GATC 6 cut(s) 182, 217, 831, 1117, 1322, 1583
MboII GAAGA 3 cut(s) 723, 922, 1493
MfeI CAATTG 1 cut(s) 615
MlyI GAGTC 4 cut(s) 386, 996, 1218, 1479
MmeI TCCRAC 4 cut(s) 256, 326, 916, 1477
MseI TTAA 2 cut(s) 686, 1376
MspR9I CCNGG 3 cut(s) 117, 261, 822
MunI CAATTG 1 cut(s) 615
Mva1269I GAATGC 1 cut(s) 1623
MvaI CCWGG 3 cut(s) 117, 261, 822
MvnI CGCG 1 cut(s) 293
MwoI GCNNNNNNNGC 5 cut(s) 47, 290, 543, 788, 980
NdeII GATC 6 cut(s) 182, 217, 831, 1117, 1322, 1583
NlaIII CATG 5 cut(s) 683, 983, 1039, 1049, 1298
NlaIV GGNNCC 1 cut(s) 485
NmuCI GTSAC 4 cut(s) 378, 988, 1073, 1256
NspV TTCGAA 1 cut(s) 705
PaqCI CACCTGC 1 cut(s) 391
PcsI WCGNNNNNNNCGW 2 cut(s) 438, 852
PctI GAATGC 1 cut(s) 1623
PfeI GAWTC 2 cut(s) 1124, 1385
PflFI GACNNNGTC 1 cut(s) 1079
PkrI GCNGC 5 cut(s) 9, 40, 1010, 1314, 1575
PleI GAGTC 4 cut(s) 385, 995, 1217, 1478
PpsI GAGTC 4 cut(s) 385, 995, 1217, 1478
PpuMI RGGWCCY 1 cut(s) 257
PshAI GACNNNNGTC 1 cut(s) 719
PshBI ATTAAT 1 cut(s) 686
Psp5II RGGWCCY 1 cut(s) 257
Psp6I CCWGG 3 cut(s) 115, 259, 820
PspEI GGTNACC 1 cut(s) 1073
PspGI CCWGG 3 cut(s) 115, 259, 820
PspN4I GGNNCC 1 cut(s) 485
PspPI GGNCC 3 cut(s) 257, 299, 767
PspPPI RGGWCCY 1 cut(s) 257
PsrI GAACNNNNNNTAC 2 cut(s) 1187, 1219
PstI CTGCAG 1 cut(s) 388
PstNI CAGNNNCTG 2 cut(s) 1212, 1473
PsyI GACNNNGTC 1 cut(s) 1079
RsaI GTAC 4 cut(s) 668, 966, 1147, 1408
RsaNI GTAC 4 cut(s) 667, 965, 1146, 1407
SaqAI TTAA 2 cut(s) 686, 1376
SatI GCNGC 5 cut(s) 8, 39, 1009, 1313, 1574
Sau3AI GATC 6 cut(s) 182, 217, 831, 1117, 1322, 1583
Sau96I GGNCC 3 cut(s) 257, 299, 767
SbfI CCTGCAGG 1 cut(s) 388
ScaI AGTACT 1 cut(s) 668
SchI GAGTC 4 cut(s) 386, 996, 1218, 1479
ScrFI CCNGG 3 cut(s) 117, 261, 822
SdaI CCTGCAGG 1 cut(s) 388
SfaNI GCATC 5 cut(s) 637, 685, 778, 1047, 1510
SfcI CTRYAG 1 cut(s) 384
SfuI TTCGAA 1 cut(s) 705
SinI GGWCC 3 cut(s) 257, 299, 767
SmlI CTYRAG 3 cut(s) 329, 464, 1025
SmoI CTYRAG 3 cut(s) 329, 464, 1025
Sse8387I CCTGCAGG 1 cut(s) 388
SsiI CCGC 2 cut(s) 39, 608
StyD4I CCNGG 3 cut(s) 115, 259, 820
TaaI ACNGT 7 cut(s) 55, 163, 907, 940, 1112, 1256, 1517
TaiI ACGT 2 cut(s) 849, 1200
TaqI TCGA 3 cut(s) 705, 855, 876
TatI WGTACW 4 cut(s) 666, 964, 1145, 1406
TauI GCSGC 1 cut(s) 41
TfiI GAWTC 2 cut(s) 1124, 1385
Tru1I TTAA 2 cut(s) 686, 1376
Tru9I TTAA 2 cut(s) 686, 1376
TscAI CASTG 7 cut(s) 912, 1117, 1161, 1261, 1368, 1422, 1522
TseFI GTSAC 4 cut(s) 378, 988, 1073, 1256
TseI GCWGC 4 cut(s) 7, 1008, 1312, 1573
Tsp45I GTSAC 4 cut(s) 378, 988, 1073, 1256
TspDTI ATGAA 9 cut(s) 183, 258, 277, 948, 968, 1052, 1062, 1311, 1593
TspGWI ACGGA 9 cut(s) 89, 312, 862, 1029, 1165, 1179, 1426, 1440, 1516
TspRI CASTG 7 cut(s) 912, 1117, 1161, 1261, 1368, 1422, 1522
Tth111I GACNNNGTC 1 cut(s) 1079
VpaK11BI GGWCC 3 cut(s) 257, 299, 767
VspI ATTAAT 1 cut(s) 686
XagI CCTNNNNNAGG 1 cut(s) 15
XapI RAATTY 5 cut(s) 491, 635, 709, 738, 1092
XcmI CCANNNNNNNNNTGG 1 cut(s) 666
XmiI GTMKAC 2 cut(s) 1251, 1512
ZrmI AGTACT 1 cut(s) 668
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.