MD09G1116300.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0003154
Physical Location & Seq
Reverse (-)
8936441 .. 8937139
699 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1116300.v1.1.491

Sequence Viewer

Length: 699 bp
ATGAAGAAGCTCTACAACAAGAAGGGCAAAATCCACCCGTCACCGTCGCCGCCCTCCGTAGCTGACCCTCTGACCCTTCTTCCGGCAACCATCCTTGCCTTGGCCATATCTCTTTCCGCCGAAGACCAAGAGGTCTTGGCCTATCTCATCTCCTGCACCAACAACTCCTCAGGCGGTTGCACGATGAACCAGCTTGGGCCCACTACTAAGTCCAATCCTGCACCGGCGACAAGACCGCCGCAGGTAACATCTATGCCTTATATCAAGCCACACAATCCCAACGCGGACAAGACCTATGGCGACCACAAGGCCCACCAGAAGTGCGACTGCTTCAGGTGCTACACGAGTTTTTGGGCACGATGGGACGCTTCGCCGAACCGCCAGCTGATCCACGAAATCATTGAGGCTTACGAGGAGAGGTTGGTGGAGAAGAAGAGGTTAAGAAACTCAAAGGTGAAGAAAGAGAGAAGGAAGACTAACAAGGGCAGTGGTGTTCATTTGGGCTCTGGGGAATTAGGGCATGGGGACAAGAAGGAGGTGGAGGTAGGCCCACAAATGGTGGATGCTGGTGTTGTTGTTGGTGAAGTGATGAGAGGTGGTGAGTCTGGTGATGAAGGTTGTGAGGTTGGAGTGGAGAGGGGGACCATAAGGAGGCTGGTGAGTTTCATTGGGGACAAGATTTGGGGTGCTTGGAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

233

Amino Acids

25.46

Weight (kDa)

9.07

Isoelectric Point (pI)

44.03

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 131
Acc36I ACCTGC 1 cut(s) 232
AccII CGCG 1 cut(s) 284
AciI CCGC 7 cut(s) 50, 117, 174, 236, 239, 284, 379
AclWI GGATC 1 cut(s) 382
AcoI YGGCCR 1 cut(s) 102
AcuI CTGAAG 1 cut(s) 316
AfiI CCNNNNNNNGG 4 cut(s) 82, 100, 556, 651
AluBI AGCT 4 cut(s) 10, 62, 193, 385
AluI AGCT 4 cut(s) 10, 62, 193, 385
AlwI GGATC 1 cut(s) 382
AoxI GGCC 5 cut(s) 102, 138, 197, 309, 547
ApaI GGGCCC 1 cut(s) 201
AspS9I GGNCC 5 cut(s) 197, 198, 310, 548, 642
AsuHPI GGTGA 6 cut(s) 33, 466, 593, 611, 620, 670
AvaII GGWCC 1 cut(s) 642
AxyI CCTNAGG 1 cut(s) 169
BaeGI GKGCMC 2 cut(s) 201, 358
BalI TGGCCA 1 cut(s) 104
BanII GRGCYC 2 cut(s) 201, 506
BarI GAAGNNNNNNTAC 1 cut(s) 28
BauI CACGAG 1 cut(s) 343
BbsI GAAGAC 2 cut(s) 129, 479
BccI CCATC 2 cut(s) 98, 354
BfuAI ACCTGC 1 cut(s) 232
BisI GCNGC 2 cut(s) 50, 239
BlsI GCNGC 2 cut(s) 51, 240
Bme18I GGWCC 1 cut(s) 642
BmgT120I GGNCC 5 cut(s) 197, 198, 310, 548, 642
BmiI GGNNCC 2 cut(s) 199, 643
BmsI GCATC 1 cut(s) 553
BpiI GAAGAC 2 cut(s) 129, 479
BsaJI CCNNGG 1 cut(s) 99
Bsc4I CCNNNNNNNGG 4 cut(s) 82, 100, 556, 651
Bse118I RCCGGY 1 cut(s) 223
Bse21I CCTNAGG 1 cut(s) 169
BseDI CCNNGG 1 cut(s) 99
BseGI GGATG 2 cut(s) 90, 568
BseLI CCNNNNNNNGG 4 cut(s) 82, 100, 556, 651
BseMII CTCAG 1 cut(s) 183
BseRI GAGGAG 2 cut(s) 157, 428
BseSI GKGCMC 2 cut(s) 201, 358
BsgI GTGCAG 2 cut(s) 139, 204
Bsh1236I CGCG 1 cut(s) 284
BshFI GGCC 5 cut(s) 104, 140, 199, 311, 549
BsiSI CCGG 2 cut(s) 83, 224
BslFI GGGAC 4 cut(s) 377, 539, 655, 686
BslI CCNNNNNNNGG 4 cut(s) 82, 100, 556, 651
BsmFI GGGAC 4 cut(s) 377, 539, 655, 686
BsnI GGCC 5 cut(s) 104, 140, 199, 311, 549
Bsp120I GGGCCC 1 cut(s) 197
Bsp1286I GDGCHC 3 cut(s) 201, 358, 506
Bsp143I GATC 1 cut(s) 387
BspACI CCGC 7 cut(s) 50, 117, 174, 236, 239, 284, 379
BspANI GGCC 5 cut(s) 104, 140, 199, 311, 549
BspCNI CTCAG 1 cut(s) 182
BspFNI CGCG 1 cut(s) 284
BspLI GGNNCC 2 cut(s) 199, 643
BspMI ACCTGC 1 cut(s) 232
BspPI GGATC 1 cut(s) 382
BsrFI RCCGGY 1 cut(s) 223
BssAI RCCGGY 1 cut(s) 223
BssECI CCNNGG 1 cut(s) 99
BssMI GATC 1 cut(s) 387
BssSI CACGAG 1 cut(s) 343
BssT1I CCWWGG 1 cut(s) 99
Bst2BI CACGAG 1 cut(s) 343
Bst4CI ACNGT 1 cut(s) 45
Bst6I CTCTTC 1 cut(s) 428
BstC8I GCNNGC 1 cut(s) 383
BstDEI CTNAG 2 cut(s) 169, 207
BstF5I GGATG 2 cut(s) 90, 568
BstFNI CGCG 1 cut(s) 284
BstKTI GATC 1 cut(s) 390
BstMBI GATC 1 cut(s) 387
BstMWI GCNNNNNNNGC 1 cut(s) 336
BstSLI GKGCMC 2 cut(s) 201, 358
BstUI CGCG 1 cut(s) 284
BstV2I GAAGAC 2 cut(s) 129, 479
Bsu36I CCTNAGG 1 cut(s) 169
BsuRI GGCC 5 cut(s) 104, 140, 199, 311, 549
BtsCI GGATG 2 cut(s) 90, 568
BtsI GCAGTG 1 cut(s) 493
BtsIMutI CAGTG 1 cut(s) 493
BveI ACCTGC 1 cut(s) 232
Cac8I GCNNGC 1 cut(s) 383
Cfr10I RCCGGY 1 cut(s) 223
Cfr13I GGNCC 5 cut(s) 197, 198, 310, 548, 642
CseI GACGC 1 cut(s) 374
CspCI CAANNNNNGTGG 2 cut(s) 469, 504
CviAII CATG 1 cut(s) 521
DdeI CTNAG 2 cut(s) 169, 207
DpnI GATC 1 cut(s) 389
DpnII GATC 1 cut(s) 387
DrdI GACNNNNNNGTC 1 cut(s) 131
DseDI GACNNNNNNGTC 1 cut(s) 131
EaeI YGGCCR 1 cut(s) 102
Eam1104I CTCTTC 1 cut(s) 428
EarI CTCTTC 1 cut(s) 428
EciI GGCGGA 1 cut(s) 106
Eco130I CCWWGG 1 cut(s) 99
Eco24I GRGCYC 2 cut(s) 201, 506
Eco47I GGWCC 1 cut(s) 642
Eco57I CTGAAG 1 cut(s) 316
Eco81I CCTNAGG 1 cut(s) 169
EcoT14I CCWWGG 1 cut(s) 99
EcoT38I GRGCYC 2 cut(s) 201, 506
ErhI CCWWGG 1 cut(s) 99
FaeI CATG 1 cut(s) 524
FaiI YATR 6 cut(s) 107, 254, 261, 297, 522, 647
FaqI GGGAC 4 cut(s) 377, 539, 655, 686
FatI CATG 1 cut(s) 520
Fnu4HI GCNGC 2 cut(s) 50, 239
FokI GGATG 2 cut(s) 77, 575
FriOI GRGCYC 2 cut(s) 201, 506
Fsp4HI GCNGC 2 cut(s) 50, 239
GluI GCNGC 2 cut(s) 50, 239
HaeIII GGCC 5 cut(s) 104, 140, 199, 311, 549
HapII CCGG 2 cut(s) 83, 224
HgaI GACGC 1 cut(s) 374
Hin1II CATG 1 cut(s) 524
HinfI GANTC 1 cut(s) 602
HpaII CCGG 2 cut(s) 83, 224
HphI GGTGA 6 cut(s) 33, 466, 593, 611, 620, 670
Hpy188I TCNGA 1 cut(s) 72
Hpy99I CGWCG 1 cut(s) 49
HpyAV CCTTC 5 cut(s) 16, 86, 462, 526, 608
HpyCH4III ACNGT 1 cut(s) 45
HpyCH4V TGCA 3 cut(s) 156, 180, 221
HpyF10VI GCNNNNNNNGC 1 cut(s) 336
HpyF3I CTNAG 2 cut(s) 169, 207
Hsp92II CATG 1 cut(s) 524
Kzo9I GATC 1 cut(s) 387
LweI GCATC 1 cut(s) 553
MaeIII GTNAC 2 cut(s) 39, 244
MalI GATC 1 cut(s) 389
MboI GATC 1 cut(s) 387
MboII GAAGA 7 cut(s) 16, 71, 134, 442, 445, 469, 484
MhlI GDGCHC 3 cut(s) 201, 358, 506
MlsI TGGCCA 1 cut(s) 104
MluCI AATT 2 cut(s) 512, 694
MluNI TGGCCA 1 cut(s) 104
MlyI GAGTC 1 cut(s) 611
MmeI TCCRAC 1 cut(s) 607
Mox20I TGGCCA 1 cut(s) 104
MscI TGGCCA 1 cut(s) 104
MseI TTAA 1 cut(s) 440
Msp20I TGGCCA 1 cut(s) 104
MspA1I CMGCKG 1 cut(s) 385
MspI CCGG 2 cut(s) 83, 224
MvnI CGCG 1 cut(s) 284
MwoI GCNNNNNNNGC 1 cut(s) 336
NdeII GATC 1 cut(s) 387
NlaIII CATG 1 cut(s) 524
NlaIV GGNNCC 2 cut(s) 199, 643
NmuCI GTSAC 1 cut(s) 39
PkrI GCNGC 2 cut(s) 51, 240
PleI GAGTC 1 cut(s) 610
PpsI GAGTC 1 cut(s) 610
PspN4I GGNNCC 2 cut(s) 199, 643
PspOMI GGGCCC 1 cut(s) 197
PspPI GGNCC 5 cut(s) 197, 198, 310, 548, 642
PvuII CAGCTG 1 cut(s) 385
SaqAI TTAA 1 cut(s) 440
SatI GCNGC 2 cut(s) 50, 239
Sau3AI GATC 1 cut(s) 387
Sau96I GGNCC 5 cut(s) 197, 198, 310, 548, 642
SchI GAGTC 1 cut(s) 611
SduI GDGCHC 3 cut(s) 201, 358, 506
SfaNI GCATC 1 cut(s) 553
SgrAI CRCCGGYG 1 cut(s) 223
SinI GGWCC 1 cut(s) 642
Sse9I AATT 2 cut(s) 512, 694
SsiI CCGC 7 cut(s) 50, 117, 174, 236, 239, 284, 379
StyI CCWWGG 1 cut(s) 99
TaaI ACNGT 1 cut(s) 45
TasI AATT 2 cut(s) 512, 694
TauI GCSGC 2 cut(s) 52, 241
Tru1I TTAA 1 cut(s) 440
Tru9I TTAA 1 cut(s) 440
TscAI CASTG 1 cut(s) 493
TseFI GTSAC 1 cut(s) 39
Tsp45I GTSAC 1 cut(s) 39
TspDTI ATGAA 5 cut(s) 17, 200, 485, 627, 655
TspGWI ACGGA 1 cut(s) 46
TspRI CASTG 1 cut(s) 493
VpaK11BI GGWCC 1 cut(s) 642
XcmI CCANNNNNNNNNTGG 2 cut(s) 97, 652
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.