Prupe.4G012500_v2.0.a1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0003154
Physical Location & Seq
Forward (+)
592073 .. 593846
1774 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G012500.1

Sequence Viewer

Length: 654 bp
ATGAAGAAGCTCTACCGGAAGGGCACGGTTCATCCTTCACCGCCAATCATATCAGACCACCTAGCCTTCCTTCCCGCTACCATCCTCACCCTCGCGGCGGCTCTGTCGCCCGAGGACAGAGAGGTCTTGGCTTACCTCATATCCTGCTCCAACAGCTCTGTCAACTTATCATCATCGTCGTACGGTTCACGTAAGTCCACCACAGCCTCTGCAGCCAAAAAGGGCCCTGCAAAAGGCAGTGGGAGCGGCGGTGACCACCCGGCGCGGTTTAACTGCGACTGCTTCAGGTGCTACAAGAGTTACTGGGTCAGATGGGACGAGTCGCCGAATCGGCAACTGATCCACGAGATCATCGATGCTTTTGAAGATGGGGTGTTGGCTGAGAGTAAAGGGCAGGCAAACTCAAAGTATAATAATAATAACAGTAGGAAGGAGAGGAGGAACAAGAGGGGTCATAATGGTGGGTCATCGGGTGAGTTGAAGCGGTCCGAGTTGAGTTTGAAGAAGAAGGAGCTGGCTCAGGCTCACATGGCGGATCAAGAGACTAGTGGTGGTCGTGGTGGAGAGGTTGATGAAGAAGAGTCAGAGAAGAGTTCAGTGAGAAGGTTTGTGAGCTTTGTTGGAGAGAGGATATGGGGTGTGTGGAGCCAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

218

Amino Acids

23.9

Weight (kDa)

9.24

Isoelectric Point (pI)

53.64

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 122
AccBSI CCGCTC 1 cut(s) 246
AccII CGCG 2 cut(s) 95, 265
AciI CCGC 9 cut(s) 41, 75, 95, 98, 246, 249, 265, 484, 533
AclWI GGATC 2 cut(s) 334, 543
AcuI CTGAAG 1 cut(s) 268
AfaI GTAC 1 cut(s) 182
AfiI CCNNNNNNNGG 2 cut(s) 97, 233
AgsI TTSAA 3 cut(s) 365, 481, 502
AhlI ACTAGT 1 cut(s) 545
AluBI AGCT 4 cut(s) 10, 156, 514, 615
AluI AGCT 4 cut(s) 10, 156, 514, 615
Alw26I GTCTC 1 cut(s) 536
AlwI GGATC 2 cut(s) 334, 543
AlwNI CAGNNNCTG 1 cut(s) 209
Ama87I CYCGRG 1 cut(s) 110
AoxI GGCC 1 cut(s) 223
ApaI GGGCCC 1 cut(s) 227
ApeKI GCWGC 1 cut(s) 212
AspLEI GCGC 1 cut(s) 265
AspS9I GGNCC 3 cut(s) 223, 224, 486
AsuC2I CCSGG 1 cut(s) 260
AsuHPI GGTGA 4 cut(s) 30, 79, 263, 485
AvaI CYCGRG 1 cut(s) 110
AvaII GGWCC 1 cut(s) 486
BaeGI GKGCMC 2 cut(s) 26, 227
BanII GRGCYC 1 cut(s) 227
BarI GAAGNNNNNNTAC 1 cut(s) 28
BauI CACGAG 1 cut(s) 344
BbvI GCAGC 1 cut(s) 224
BccI CCATC 3 cut(s) 89, 306, 362
BcnI CCSGG 1 cut(s) 260
BcoDI GTCTC 1 cut(s) 536
BcuI ACTAGT 1 cut(s) 545
BfaI CTAG 2 cut(s) 62, 546
BfmI CTRYAG 1 cut(s) 210
BglI GCCNNNNNGGC 1 cut(s) 331
BisI GCNGC 4 cut(s) 96, 99, 213, 247
BlsI GCNGC 4 cut(s) 97, 100, 214, 248
Bme1390I CCNGG 1 cut(s) 260
Bme18I GGWCC 1 cut(s) 486
BmeT110I CYCGRG 1 cut(s) 110
BmgT120I GGNCC 3 cut(s) 223, 224, 486
BmiI GGNNCC 2 cut(s) 225, 647
BmrFI CCNGG 1 cut(s) 260
BmrI ACTGGG 1 cut(s) 313
BmsI GCATC 1 cut(s) 346
BmuI ACTGGG 1 cut(s) 313
Bpu10I CCTNAGC 1 cut(s) 519
BpuMI CCSGG 1 cut(s) 260
Bsa29I ATCGAT 1 cut(s) 354
BsaAI YACGTR 1 cut(s) 191
BsaJI CCNNGG 1 cut(s) 111
BsaWI WCCGGW 1 cut(s) 15
BsaXI ACNNNNNCTCC 2 cut(s) 235, 265
Bsc4I CCNNNNNNNGG 2 cut(s) 97, 233
Bse1I ACTGG 1 cut(s) 308
BseCI ATCGAT 1 cut(s) 354
BseDI CCNNGG 1 cut(s) 111
BseGI GGATG 2 cut(s) 31, 81
BseLI CCNNNNNNNGG 2 cut(s) 97, 233
BseMII CTCAG 2 cut(s) 372, 533
BseNI ACTGG 1 cut(s) 308
BseRI GAGGAG 1 cut(s) 451
BseSI GKGCMC 2 cut(s) 26, 227
BseXI GCAGC 1 cut(s) 224
Bsh1236I CGCG 2 cut(s) 95, 265
BshFI GGCC 1 cut(s) 225
BshVI ATCGAT 1 cut(s) 354
BsiHKCI CYCGRG 1 cut(s) 110
BsiSI CCGG 2 cut(s) 16, 260
BsiWI CGTACG 1 cut(s) 180
BslFI GGGAC 1 cut(s) 329
BslI CCNNNNNNNGG 2 cut(s) 97, 233
BsmAI GTCTC 1 cut(s) 536
BsmFI GGGAC 1 cut(s) 329
BsnI GGCC 1 cut(s) 225
BsoBI CYCGRG 1 cut(s) 110
Bsp120I GGGCCC 1 cut(s) 223
Bsp1286I GDGCHC 2 cut(s) 26, 227
Bsp143I GATC 3 cut(s) 339, 348, 535
BspACI CCGC 9 cut(s) 41, 75, 95, 98, 246, 249, 265, 484, 533
BspANI GGCC 1 cut(s) 225
BspCNI CTCAG 2 cut(s) 373, 532
BspDI ATCGAT 1 cut(s) 354
BspFNI CGCG 2 cut(s) 95, 265
BspLI GGNNCC 2 cut(s) 225, 647
BspMAI CTGCAG 1 cut(s) 214
BspPI GGATC 2 cut(s) 334, 543
BsrBI CCGCTC 1 cut(s) 246
BsrI ACTGG 1 cut(s) 308
BssECI CCNNGG 1 cut(s) 111
BssMI GATC 3 cut(s) 339, 348, 535
BssSI CACGAG 1 cut(s) 344
Bst2BI CACGAG 1 cut(s) 344
Bst4CI ACNGT 3 cut(s) 28, 185, 425
Bst6I CTCTTC 2 cut(s) 573, 584
BstBAI YACGTR 1 cut(s) 191
BstC8I GCNNGC 2 cut(s) 396, 516
BstDEI CTNAG 2 cut(s) 381, 519
BstEII GGTNACC 1 cut(s) 251
BstENI CCTNNNNNAGG 1 cut(s) 231
BstF5I GGATG 2 cut(s) 31, 81
BstFNI CGCG 2 cut(s) 95, 265
BstHHI GCGC 1 cut(s) 265
BstKTI GATC 3 cut(s) 342, 351, 538
BstMAI GTCTC 1 cut(s) 536
BstMBI GATC 3 cut(s) 339, 348, 535
BstMWI GCNNNNNNNGC 6 cut(s) 153, 212, 243, 288, 331, 530
BstPI GGTNACC 1 cut(s) 251
BstSCI CCNGG 1 cut(s) 258
BstSFI CTRYAG 1 cut(s) 210
BstSLI GKGCMC 2 cut(s) 26, 227
BstUI CGCG 2 cut(s) 95, 265
BstV1I GCAGC 1 cut(s) 224
Bsu15I ATCGAT 1 cut(s) 354
BsuRI GGCC 1 cut(s) 225
BsuTUI ATCGAT 1 cut(s) 354
BtsCI GGATG 2 cut(s) 31, 81
BtsI GCAGTG 1 cut(s) 244
BtsIMutI CAGTG 2 cut(s) 244, 603
Cac8I GCNNGC 2 cut(s) 396, 516
CaiI CAGNNNCTG 1 cut(s) 209
CfoI GCGC 1 cut(s) 265
Cfr13I GGNCC 3 cut(s) 223, 224, 486
ClaI ATCGAT 1 cut(s) 354
CpoI CGGWCCG 1 cut(s) 486
Csp6I GTAC 1 cut(s) 181
CspI CGGWCCG 1 cut(s) 486
CviAII CATG 1 cut(s) 529
CviQI GTAC 1 cut(s) 181
DdeI CTNAG 2 cut(s) 381, 519
DpnI GATC 3 cut(s) 341, 350, 537
DpnII GATC 3 cut(s) 339, 348, 535
DrdI GACNNNNNNGTC 1 cut(s) 122
DseDI GACNNNNNNGTC 1 cut(s) 122
Eam1104I CTCTTC 2 cut(s) 573, 584
EarI CTCTTC 2 cut(s) 573, 584
EciI GGCGGA 1 cut(s) 548
Eco24I GRGCYC 1 cut(s) 227
Eco47I GGWCC 1 cut(s) 486
Eco57I CTGAAG 1 cut(s) 268
Eco88I CYCGRG 1 cut(s) 110
Eco91I GGTNACC 1 cut(s) 251
EcoNI CCTNNNNNAGG 1 cut(s) 231
EcoO109I RGGNCCY 2 cut(s) 223, 224
EcoO65I GGTNACC 1 cut(s) 251
EcoT38I GRGCYC 1 cut(s) 227
FaeI CATG 1 cut(s) 532
FaiI YATR 6 cut(s) 50, 140, 411, 456, 530, 634
FaqI GGGAC 1 cut(s) 329
FatI CATG 1 cut(s) 528
FauI CCCGC 1 cut(s) 82
Fnu4HI GCNGC 4 cut(s) 96, 99, 213, 247
FokI GGATG 2 cut(s) 18, 68
FriOI GRGCYC 1 cut(s) 227
Fsp4HI GCNGC 4 cut(s) 96, 99, 213, 247
FspBI CTAG 2 cut(s) 62, 546
GlaI GCGC 1 cut(s) 264
GluI GCNGC 4 cut(s) 96, 99, 213, 247
HaeIII GGCC 1 cut(s) 225
HapII CCGG 2 cut(s) 16, 260
HhaI GCGC 1 cut(s) 265
Hin1II CATG 1 cut(s) 532
Hin6I GCGC 1 cut(s) 263
HinP1I GCGC 1 cut(s) 263
HincII GTYRAC 1 cut(s) 163
HindII GTYRAC 1 cut(s) 163
HinfI GANTC 3 cut(s) 320, 328, 581
HpaII CCGG 2 cut(s) 16, 260
HphI GGTGA 4 cut(s) 30, 79, 263, 485
Hpy166II GTNNAC 3 cut(s) 163, 188, 198
Hpy188I TCNGA 4 cut(s) 55, 311, 490, 586
Hpy188III TCNNGA 1 cut(s) 539
Hpy8I GTNNAC 3 cut(s) 163, 188, 198
Hpy99I CGWCG 1 cut(s) 181
HpyAV CCTTC 7 cut(s) 13, 45, 76, 80, 424, 502, 597
HpyCH4III ACNGT 3 cut(s) 28, 185, 425
HpyCH4IV ACGT 1 cut(s) 190
HpyCH4V TGCA 2 cut(s) 212, 230
HpyF10VI GCNNNNNNNGC 6 cut(s) 153, 212, 243, 288, 331, 530
HpyF3I CTNAG 2 cut(s) 381, 519
HpySE526I ACGT 1 cut(s) 190
Hsp92II CATG 1 cut(s) 532
HspAI GCGC 1 cut(s) 263
Kzo9I GATC 3 cut(s) 339, 348, 535
LmnI GCTCC 4 cut(s) 152, 243, 511, 645
LpnPI CCDG 9 cut(s) 29, 157, 240, 271, 273, 289, 380, 500, 506
Lsp1109I GCAGC 1 cut(s) 224
LweI GCATC 1 cut(s) 346
MaeI CTAG 2 cut(s) 62, 546
MaeII ACGT 1 cut(s) 190
MaeIII GTNAC 2 cut(s) 251, 299
MalI GATC 3 cut(s) 341, 350, 537
MbiI CCGCTC 1 cut(s) 246
MboI GATC 3 cut(s) 339, 348, 535
MboII GAAGA 7 cut(s) 16, 377, 514, 517, 587, 590, 601
MhlI GDGCHC 2 cut(s) 26, 227
MlyI GAGTC 2 cut(s) 329, 590
MmeI TCCRAC 2 cut(s) 174, 601
MseI TTAA 1 cut(s) 270
MslI CAYNNNNRTG 1 cut(s) 459
MspI CCGG 2 cut(s) 16, 260
MspR9I CCNGG 1 cut(s) 260
MvnI CGCG 2 cut(s) 95, 265
MwoI GCNNNNNNNGC 6 cut(s) 153, 212, 243, 288, 331, 530
NciI CCSGG 1 cut(s) 260
NdeII GATC 3 cut(s) 339, 348, 535
NlaIII CATG 1 cut(s) 532
NlaIV GGNNCC 2 cut(s) 225, 647
NmuCI GTSAC 1 cut(s) 251
PcsI WCGNNNNNNNCGW 1 cut(s) 351
PfeI GAWTC 1 cut(s) 328
Pfl23II CGTACG 1 cut(s) 180
PkrI GCNGC 4 cut(s) 97, 100, 214, 248
PleI GAGTC 2 cut(s) 328, 589
PpsI GAGTC 2 cut(s) 328, 589
Ppu21I YACGTR 1 cut(s) 191
PspEI GGTNACC 1 cut(s) 251
PspLI CGTACG 1 cut(s) 180
PspN4I GGNNCC 2 cut(s) 225, 647
PspOMI GGGCCC 1 cut(s) 223
PspPI GGNCC 3 cut(s) 223, 224, 486
PstI CTGCAG 1 cut(s) 214
PstNI CAGNNNCTG 1 cut(s) 209
RsaI GTAC 1 cut(s) 182
RsaNI GTAC 1 cut(s) 181
RseI CAYNNNNRTG 1 cut(s) 459
Rsr2I CGGWCCG 1 cut(s) 486
RsrII CGGWCCG 1 cut(s) 486
SaqAI TTAA 1 cut(s) 270
SatI GCNGC 4 cut(s) 96, 99, 213, 247
Sau3AI GATC 3 cut(s) 339, 348, 535
Sau96I GGNCC 3 cut(s) 223, 224, 486
SchI GAGTC 2 cut(s) 329, 590
ScrFI CCNGG 1 cut(s) 260
SduI GDGCHC 2 cut(s) 26, 227
SfaNI GCATC 1 cut(s) 346
SfcI CTRYAG 1 cut(s) 210
SinI GGWCC 1 cut(s) 486
SmiMI CAYNNNNRTG 1 cut(s) 459
SpeI ACTAGT 1 cut(s) 545
SsiI CCGC 9 cut(s) 41, 75, 95, 98, 246, 249, 265, 484, 533
SspMI CTAG 2 cut(s) 62, 546
StyD4I CCNGG 1 cut(s) 258
TaaI ACNGT 3 cut(s) 28, 185, 425
TaiI ACGT 1 cut(s) 193
TaqI TCGA 1 cut(s) 354
TauI GCSGC 3 cut(s) 98, 101, 249
TfiI GAWTC 1 cut(s) 328
Tru1I TTAA 1 cut(s) 270
Tru9I TTAA 1 cut(s) 270
TscAI CASTG 2 cut(s) 244, 603
TseFI GTSAC 1 cut(s) 251
TseI GCWGC 1 cut(s) 212
Tsp45I GTSAC 1 cut(s) 251
TspDTI ATGAA 3 cut(s) 17, 20, 588
TspRI CASTG 2 cut(s) 244, 603
VpaK11BI GGWCC 1 cut(s) 486
XagI CCTNNNNNAGG 1 cut(s) 231
XspI CTAG 2 cut(s) 62, 546
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.