Rh2CG513000

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0003154
Physical Location & Seq
Forward (+)
68694980 .. 68695570
591 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG513000.1

Sequence Viewer

Length: 591 bp
ATGAAGAAGCTCTACAACAAGAAAGGCAAGGTCCACCCGTCGCCACCGCCTTCGACACCCGACCCGTTCGCCCTGCTTCCGGCCACCATTTTGACTCTGACCACCGCCCTCTCTGTCCAGGACCGAGAGGTCCTCGCCTATCTCATCTCCTACACCAACAACAACAATTCGACCAGCATCAACACCAACAAGGCCAACGATGCCCATAAAGCTGGAAATGATCACCCTGATCCTCCGATGTCCCAGTGCGACTGCTTTAGGTGCTACAAGAGCTTCTGGGCACGATGGGATGCTTCCCCCAACCGGCAGCTCATACATGAAATCATCGAGGCGTACGAGGAAAAGTTGGCTGAGAAGAAAAGGAGAAATCCCAAGGTGAAGAAAGTGAGGAAGAAGAAAATTGAGGAGGAAACGAAGGGTAATATTGATAATCTACCGGTCATGAAAGAGGAGCTGAGTAATAATCCAGAAGTGGATGTGGAAAACTGTGATGGTGATGAAGAAGGCTGTGAGGTTGAGGTTGAGAAAGGATCCTCCATGAGGAGGTTGGCGAGCTTCATTGGGGATAGGATTTGGGGTGTTTGGAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

196

Amino Acids

22.31

Weight (kDa)

6.9

Isoelectric Point (pI)

52.18

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 128
AciI CCGC 2 cut(s) 47, 105
AclWI GGATC 3 cut(s) 224, 525, 538
AcoI YGGCCR 1 cut(s) 81
AfaI GTAC 1 cut(s) 335
AfiI CCNNNNNNNGG 4 cut(s) 79, 303, 540, 543
AgeI ACCGGT 1 cut(s) 436
AjnI CCWGG 1 cut(s) 117
AluBI AGCT 6 cut(s) 10, 212, 273, 310, 454, 555
AluI AGCT 6 cut(s) 10, 212, 273, 310, 454, 555
AlwI GGATC 3 cut(s) 224, 525, 538
AoxI GGCC 2 cut(s) 81, 192
ApeKI GCWGC 1 cut(s) 307
AsiGI ACCGGT 1 cut(s) 436
AspS9I GGNCC 3 cut(s) 31, 121, 130
AsuHPI GGTGA 3 cut(s) 215, 388, 506
AvaII GGWCC 3 cut(s) 31, 121, 130
BaeGI GKGCMC 1 cut(s) 283
BamHI GGATCC 1 cut(s) 530
BarI GAAGNNNNNNTAC 1 cut(s) 28
BbvI GCAGC 1 cut(s) 319
BccI CCATC 2 cut(s) 279, 485
BciT130I CCWGG 1 cut(s) 119
BclI TGATCA 1 cut(s) 220
BisI GCNGC 1 cut(s) 308
BlsI GCNGC 1 cut(s) 309
Bme1390I CCNGG 1 cut(s) 119
Bme18I GGWCC 3 cut(s) 31, 121, 130
BmgT120I GGNCC 3 cut(s) 31, 121, 130
BmiI GGNNCC 1 cut(s) 532
BmrFI CCNGG 1 cut(s) 119
BmrI ACTGGG 1 cut(s) 238
BmsI GCATC 3 cut(s) 186, 190, 280
BmuI ACTGGG 1 cut(s) 238
BplI GAGNNNNNCTC 2 cut(s) 117, 149
BsaJI CCNNGG 1 cut(s) 372
BsaWI WCCGGW 1 cut(s) 436
Bsc4I CCNNNNNNNGG 4 cut(s) 79, 303, 540, 543
Bse118I RCCGGY 2 cut(s) 303, 436
Bse1I ACTGG 1 cut(s) 244
BseBI CCWGG 1 cut(s) 119
BseDI CCNNGG 1 cut(s) 372
BseGI GGATG 2 cut(s) 295, 481
BseLI CCNNNNNNNGG 4 cut(s) 79, 303, 540, 543
BseMII CTCAG 2 cut(s) 342, 446
BseNI ACTGG 1 cut(s) 244
BseRI GAGGAG 3 cut(s) 419, 464, 556
BseSI GKGCMC 1 cut(s) 283
BseXI GCAGC 1 cut(s) 319
BshFI GGCC 2 cut(s) 83, 194
BshTI ACCGGT 1 cut(s) 436
BsiSI CCGG 3 cut(s) 80, 304, 437
BsiWI CGTACG 1 cut(s) 333
BslFI GGGAC 1 cut(s) 226
BslI CCNNNNNNNGG 4 cut(s) 79, 303, 540, 543
BsmFI GGGAC 1 cut(s) 226
BsnI GGCC 2 cut(s) 83, 194
Bsp1286I GDGCHC 1 cut(s) 283
Bsp143I GATC 3 cut(s) 220, 229, 530
BspACI CCGC 2 cut(s) 47, 105
BspANI GGCC 2 cut(s) 83, 194
BspCNI CTCAG 2 cut(s) 343, 447
BspHI TCATGA 1 cut(s) 441
BspLI GGNNCC 1 cut(s) 532
BspPI GGATC 3 cut(s) 224, 525, 538
BsrFI RCCGGY 2 cut(s) 303, 436
BsrI ACTGG 1 cut(s) 244
BssAI RCCGGY 2 cut(s) 303, 436
BssECI CCNNGG 1 cut(s) 372
BssMI GATC 3 cut(s) 220, 229, 530
BssT1I CCWWGG 1 cut(s) 372
Bst2UI CCWGG 1 cut(s) 119
Bst4CI ACNGT 1 cut(s) 488
BstC8I GCNNGC 1 cut(s) 553
BstDEI CTNAG 2 cut(s) 351, 455
BstENI CCTNNNNNAGG 1 cut(s) 538
BstF5I GGATG 2 cut(s) 295, 481
BstKTI GATC 3 cut(s) 223, 232, 533
BstMBI GATC 3 cut(s) 220, 229, 530
BstMWI GCNNNNNNNGC 4 cut(s) 200, 209, 261, 270
BstNI CCWGG 1 cut(s) 119
BstSCI CCNGG 1 cut(s) 117
BstSLI GKGCMC 1 cut(s) 283
BstV1I GCAGC 1 cut(s) 319
BstX2I RGATCY 1 cut(s) 530
BstXI CCANNNNNNTGG 1 cut(s) 212
BstYI RGATCY 1 cut(s) 530
BsuRI GGCC 2 cut(s) 83, 194
BtsCI GGATG 2 cut(s) 295, 481
BtsIMutI CAGTG 1 cut(s) 251
Cac8I GCNNGC 1 cut(s) 553
CciI TCATGA 1 cut(s) 441
Cfr10I RCCGGY 2 cut(s) 303, 436
Cfr13I GGNCC 3 cut(s) 31, 121, 130
Csp6I GTAC 1 cut(s) 334
CspAI ACCGGT 1 cut(s) 436
CviAII CATG 3 cut(s) 317, 442, 538
CviQI GTAC 1 cut(s) 334
DdeI CTNAG 2 cut(s) 351, 455
DpnI GATC 3 cut(s) 222, 231, 532
DpnII GATC 3 cut(s) 220, 229, 530
DrdI GACNNNNNNGTC 1 cut(s) 128
DseDI GACNNNNNNGTC 1 cut(s) 128
EaeI YGGCCR 1 cut(s) 81
Eco130I CCWWGG 1 cut(s) 372
Eco47I GGWCC 3 cut(s) 31, 121, 130
EcoNI CCTNNNNNAGG 1 cut(s) 538
EcoO109I RGGNCCY 1 cut(s) 130
EcoRII CCWGG 1 cut(s) 117
EcoT14I CCWWGG 1 cut(s) 372
ErhI CCWWGG 1 cut(s) 372
FaeI CATG 3 cut(s) 320, 445, 541
FaiI YATR 5 cut(s) 207, 314, 318, 443, 539
FaqI GGGAC 1 cut(s) 226
FatI CATG 3 cut(s) 316, 441, 537
FbaI TGATCA 1 cut(s) 220
Fnu4HI GCNGC 1 cut(s) 308
FokI GGATG 2 cut(s) 302, 488
Fsp4HI GCNGC 1 cut(s) 308
GluI GCNGC 1 cut(s) 308
HaeIII GGCC 2 cut(s) 83, 194
HapII CCGG 3 cut(s) 80, 304, 437
Hin1II CATG 3 cut(s) 320, 445, 541
HinfI GANTC 1 cut(s) 94
HpaII CCGG 3 cut(s) 80, 304, 437
HphI GGTGA 3 cut(s) 215, 388, 506
Hpy166II GTNNAC 1 cut(s) 34
Hpy188I TCNGA 2 cut(s) 99, 237
Hpy188III TCNNGA 2 cut(s) 442, 467
Hpy8I GTNNAC 1 cut(s) 34
Hpy99I CGWCG 1 cut(s) 43
HpyAV CCTTC 3 cut(s) 60, 409, 497
HpyCH4III ACNGT 1 cut(s) 488
HpyF10VI GCNNNNNNNGC 4 cut(s) 200, 209, 261, 270
HpyF3I CTNAG 2 cut(s) 351, 455
Hsp92II CATG 3 cut(s) 320, 445, 541
Ksp22I TGATCA 1 cut(s) 220
Kzo9I GATC 3 cut(s) 220, 229, 530
LmnI GCTCC 1 cut(s) 451
Lsp1109I GCAGC 1 cut(s) 319
LweI GCATC 3 cut(s) 186, 190, 280
MalI GATC 3 cut(s) 222, 231, 532
MboI GATC 3 cut(s) 220, 229, 530
MboII GAAGA 6 cut(s) 16, 367, 391, 403, 406, 512
MflI RGATCY 1 cut(s) 530
MhlI GDGCHC 1 cut(s) 283
MluCI AATT 3 cut(s) 166, 399, 586
MlyI GAGTC 1 cut(s) 88
MspI CCGG 3 cut(s) 80, 304, 437
MspR9I CCNGG 1 cut(s) 119
MvaI CCWGG 1 cut(s) 119
MwoI GCNNNNNNNGC 4 cut(s) 200, 209, 261, 270
NdeII GATC 3 cut(s) 220, 229, 530
NlaIII CATG 3 cut(s) 320, 445, 541
NlaIV GGNNCC 1 cut(s) 532
PagI TCATGA 1 cut(s) 441
PcsI WCGNNNNNNNCGW 1 cut(s) 333
Pfl23II CGTACG 1 cut(s) 333
PfoI TCCNGGA 1 cut(s) 117
PinAI ACCGGT 1 cut(s) 436
PkrI GCNGC 1 cut(s) 309
PleI GAGTC 1 cut(s) 88
PpsI GAGTC 1 cut(s) 88
PpuMI RGGWCCY 1 cut(s) 130
Psp5II RGGWCCY 1 cut(s) 130
Psp6I CCWGG 1 cut(s) 117
PspGI CCWGG 1 cut(s) 117
PspLI CGTACG 1 cut(s) 333
PspN4I GGNNCC 1 cut(s) 532
PspPI GGNCC 3 cut(s) 31, 121, 130
PspPPI RGGWCCY 1 cut(s) 130
PsuI RGATCY 1 cut(s) 530
RsaI GTAC 1 cut(s) 335
RsaNI GTAC 1 cut(s) 334
SatI GCNGC 1 cut(s) 308
Sau3AI GATC 3 cut(s) 220, 229, 530
Sau96I GGNCC 3 cut(s) 31, 121, 130
SchI GAGTC 1 cut(s) 88
ScrFI CCNGG 1 cut(s) 119
SduI GDGCHC 1 cut(s) 283
SfaNI GCATC 3 cut(s) 186, 190, 280
SinI GGWCC 3 cut(s) 31, 121, 130
Sse9I AATT 3 cut(s) 166, 399, 586
SsiI CCGC 2 cut(s) 47, 105
SspI AATATT 1 cut(s) 424
StyD4I CCNGG 1 cut(s) 117
StyI CCWWGG 1 cut(s) 372
TaaI ACNGT 1 cut(s) 488
TaqI TCGA 3 cut(s) 53, 170, 327
TaqII GACCGA 1 cut(s) 138
TasI AATT 3 cut(s) 166, 399, 586
TscAI CASTG 1 cut(s) 251
TseI GCWGC 1 cut(s) 307
TspDTI ATGAA 5 cut(s) 17, 333, 458, 513, 547
TspRI CASTG 1 cut(s) 251
VpaK11BI GGWCC 3 cut(s) 31, 121, 130
XagI CCTNNNNNAGG 1 cut(s) 538
XcmI CCANNNNNNNNNTGG 1 cut(s) 544
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.