Rh5DG014700

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0003154
Physical Location & Seq
Forward (+)
1097889 .. 1099445
1557 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG014700.1

Sequence Viewer

Length: 696 bp
ATGAAGAAGCTGTACCGCAGAGGCACGGTTCATCCGTCACCGCCAACCGTGTCCGACCACCTGTCCTTCCTTCCCGCCACCATCCTCGCCCTCGCGGCCGCCCTGTCCCTCGAGGACAGGGAGGTCCTGGCTTACCTCATCTCCTGCTCCAACACTAACAACGTCAACTTAACATCCTCACCCTCCTCACGTAAGACTACTACCACAACCGCCACCAACAGCAGCAACAAAAAGAGTTCCGCCGCCAAAGGAGGCGGCGGAAGCGGCGGCGGGGACCACCCGGCGCGGTTCAACTGCAGCTGCTTCAGGTGCTACACGAGTTACTGGGTCAGATGGGACGAGTCACCGAATCGAGAACTGATCCATGAGATCATCGATGCTTTCGAAGATGAGCTACTTTCATCTCAGAGTGGCAGTAGTAGTAGTAGTAAAGCGGCTGGGTCGAAGTATGGTAAGAAGGACAAGAGGCACCATAACAAGAGAGGAAACAGTGGTAACGGGCCGGGTCAAGGATCGGAGTTGAGTCTGAAGAAGAAGGATCAGATGGTGAAGCCTAACATGGTGGAGGAGACTAATAGTAGTAGTAACAGTACTGACTCTGGTGATCTTGATGGTGATGAGGAAAGCAAGGGATCGGTGAGAAGATTTGTGAGCTTTCTTGGAGAGAGGATATGGGGTGGTGTCTGGAGCCAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

231

Amino Acids

24.93

Weight (kDa)

9.0

Isoelectric Point (pI)

53.24

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 122
AbsI CCTCGAGG 1 cut(s) 110
AccB1I GGYRCC 1 cut(s) 468
AccII CGCG 2 cut(s) 95, 286
AclWI GGATC 4 cut(s) 355, 520, 546, 640
AcoI YGGCCR 1 cut(s) 96
AcuI CTGAAG 2 cut(s) 289, 548
AfaI GTAC 2 cut(s) 14, 592
AfiI CCNNNNNNNGG 1 cut(s) 509
AgsI TTSAA 1 cut(s) 292
AhdI GACNNNNNGTC 1 cut(s) 61
AjnI CCWGG 1 cut(s) 126
AluBI AGCT 4 cut(s) 10, 300, 394, 654
AluI AGCT 4 cut(s) 10, 300, 394, 654
Alw26I GTCTC 1 cut(s) 563
AlwI GGATC 4 cut(s) 355, 520, 546, 640
Ama87I CYCGRG 1 cut(s) 110
AoxI GGCC 2 cut(s) 96, 500
ApeKI GCWGC 3 cut(s) 222, 297, 300
AspLEI GCGC 1 cut(s) 286
AspS9I GGNCC 3 cut(s) 124, 274, 500
AsuC2I CCSGG 2 cut(s) 281, 504
AsuHPI GGTGA 7 cut(s) 30, 171, 336, 559, 614, 626, 649
AsuII TTCGAA 1 cut(s) 384
AvaI CYCGRG 1 cut(s) 110
AvaII GGWCC 2 cut(s) 124, 274
BanI GGYRCC 1 cut(s) 468
BarI GAAGNNNNNNTAC 3 cut(s) 28, 378, 410
BauI CACGAG 1 cut(s) 316
BbvI GCAGC 3 cut(s) 234, 287, 309
BccI CCATC 4 cut(s) 89, 327, 538, 605
BciT130I CCWGG 1 cut(s) 128
BcnI CCSGG 2 cut(s) 281, 504
BcoDI GTCTC 1 cut(s) 563
BfmI CTRYAG 1 cut(s) 295
BglI GCCNNNNNGGC 1 cut(s) 95
BmcAI AGTACT 1 cut(s) 592
Bme1390I CCNGG 3 cut(s) 128, 281, 504
Bme18I GGWCC 2 cut(s) 124, 274
BmeRI GACNNNNNGTC 1 cut(s) 61
BmeT110I CYCGRG 1 cut(s) 110
BmgT120I GGNCC 3 cut(s) 124, 274, 500
BmiI GGNNCC 3 cut(s) 275, 470, 689
BmrFI CCNGG 3 cut(s) 128, 281, 504
BmrI ACTGGG 1 cut(s) 334
BmsI GCATC 1 cut(s) 367
BmuI ACTGGG 1 cut(s) 334
Bpu14I TTCGAA 1 cut(s) 384
BpuMI CCSGG 2 cut(s) 281, 504
Bsa29I ATCGAT 1 cut(s) 375
BsaAI YACGTR 1 cut(s) 191
BsaXI ACNNNNNCTCC 2 cut(s) 125, 155
Bsc4I CCNNNNNNNGG 1 cut(s) 509
Bse1I ACTGG 2 cut(s) 329, 691
BseBI CCWGG 1 cut(s) 128
BseCI ATCGAT 1 cut(s) 375
BseGI GGATG 3 cut(s) 31, 81, 173
BseLI CCNNNNNNNGG 1 cut(s) 509
BseMII CTCAG 1 cut(s) 419
BseNI ACTGG 2 cut(s) 329, 691
BseRI GAGGAG 2 cut(s) 175, 581
BseX3I CGGCCG 1 cut(s) 96
BseXI GCAGC 3 cut(s) 234, 287, 309
BseYI CCCAGC 1 cut(s) 437
Bsh1236I CGCG 2 cut(s) 95, 286
Bsh1285I CGRYCG 1 cut(s) 99
BshFI GGCC 2 cut(s) 98, 502
BshNI GGYRCC 1 cut(s) 468
BshVI ATCGAT 1 cut(s) 375
BsiEI CGRYCG 1 cut(s) 99
BsiHKCI CYCGRG 1 cut(s) 110
BsiSI CCGG 2 cut(s) 281, 503
BslFI GGGAC 3 cut(s) 91, 287, 350
BslI CCNNNNNNNGG 1 cut(s) 509
BsmAI GTCTC 1 cut(s) 563
BsmFI GGGAC 3 cut(s) 91, 287, 350
BsnI GGCC 2 cut(s) 98, 502
BsoBI CYCGRG 1 cut(s) 110
Bsp119I TTCGAA 1 cut(s) 384
Bsp143I GATC 6 cut(s) 360, 369, 512, 538, 604, 632
BspANI GGCC 2 cut(s) 98, 502
BspCNI CTCAG 1 cut(s) 418
BspDI ATCGAT 1 cut(s) 375
BspFNI CGCG 2 cut(s) 95, 286
BspLI GGNNCC 3 cut(s) 275, 470, 689
BspMAI CTGCAG 1 cut(s) 299
BspPI GGATC 4 cut(s) 355, 520, 546, 640
BspT104I TTCGAA 1 cut(s) 384
BspT107I GGYRCC 1 cut(s) 468
BsrI ACTGG 2 cut(s) 329, 691
BssMI GATC 6 cut(s) 360, 369, 512, 538, 604, 632
BssSI CACGAG 1 cut(s) 316
Bst2BI CACGAG 1 cut(s) 316
Bst2UI CCWGG 1 cut(s) 128
Bst4CI ACNGT 4 cut(s) 28, 49, 491, 590
BstBAI YACGTR 1 cut(s) 191
BstBI TTCGAA 1 cut(s) 384
BstDEI CTNAG 1 cut(s) 405
BstF5I GGATG 3 cut(s) 31, 81, 173
BstFNI CGCG 2 cut(s) 95, 286
BstHHI GCGC 1 cut(s) 286
BstKTI GATC 6 cut(s) 363, 372, 515, 541, 607, 635
BstMAI GTCTC 1 cut(s) 563
BstMBI GATC 6 cut(s) 360, 369, 512, 538, 604, 632
BstMCI CGRYCG 1 cut(s) 99
BstMWI GCNNNNNNNGC 4 cut(s) 95, 261, 264, 309
BstNI CCWGG 1 cut(s) 128
BstSCI CCNGG 3 cut(s) 126, 279, 502
BstSFI CTRYAG 1 cut(s) 295
BstUI CGCG 2 cut(s) 95, 286
BstV1I GCAGC 3 cut(s) 234, 287, 309
BstZI CGGCCG 1 cut(s) 96
Bsu15I ATCGAT 1 cut(s) 375
BsuRI GGCC 2 cut(s) 98, 502
BsuTUI ATCGAT 1 cut(s) 375
BtsCI GGATG 3 cut(s) 31, 81, 173
BtsIMutI CAGTG 1 cut(s) 496
CciNI GCGGCCGC 1 cut(s) 96
CfoI GCGC 1 cut(s) 286
Cfr13I GGNCC 3 cut(s) 124, 274, 500
ClaI ATCGAT 1 cut(s) 375
Csp6I GTAC 2 cut(s) 13, 591
CviAII CATG 2 cut(s) 365, 559
CviQI GTAC 2 cut(s) 13, 591
DdeI CTNAG 1 cut(s) 405
DpnI GATC 6 cut(s) 362, 371, 514, 540, 606, 634
DpnII GATC 6 cut(s) 360, 369, 512, 538, 604, 632
DrdI GACNNNNNNGTC 1 cut(s) 122
DriI GACNNNNNGTC 1 cut(s) 61
DseDI GACNNNNNNGTC 1 cut(s) 122
EaeI YGGCCR 1 cut(s) 96
EagI CGGCCG 1 cut(s) 96
Eam1105I GACNNNNNGTC 1 cut(s) 61
EciI GGCGGA 2 cut(s) 229, 273
EclXI CGGCCG 1 cut(s) 96
Eco47I GGWCC 2 cut(s) 124, 274
Eco52I CGGCCG 1 cut(s) 96
Eco57I CTGAAG 2 cut(s) 289, 548
Eco88I CYCGRG 1 cut(s) 110
EcoO109I RGGNCCY 1 cut(s) 124
EcoRII CCWGG 1 cut(s) 126
FaeI CATG 2 cut(s) 368, 562
FaiI YATR 5 cut(s) 366, 450, 474, 560, 673
FaqI GGGAC 3 cut(s) 91, 287, 350
FatI CATG 2 cut(s) 364, 558
FauI CCCGC 2 cut(s) 82, 263
FokI GGATG 3 cut(s) 18, 68, 160
GlaI GCGC 1 cut(s) 285
GsaI CCCAGC 1 cut(s) 441
HaeIII GGCC 2 cut(s) 98, 502
HapII CCGG 2 cut(s) 281, 503
HhaI GCGC 1 cut(s) 286
Hin1II CATG 2 cut(s) 368, 562
Hin6I GCGC 1 cut(s) 284
HinP1I GCGC 1 cut(s) 284
HincII GTYRAC 1 cut(s) 166
HindII GTYRAC 1 cut(s) 166
HinfI GANTC 4 cut(s) 341, 349, 523, 596
HpaII CCGG 2 cut(s) 281, 503
HphI GGTGA 7 cut(s) 30, 171, 336, 559, 614, 626, 649
Hpy166II GTNNAC 1 cut(s) 166
Hpy188I TCNGA 6 cut(s) 55, 332, 408, 517, 528, 543
Hpy188III TCNNGA 3 cut(s) 353, 608, 685
Hpy8I GTNNAC 1 cut(s) 166
HpyAV CCTTC 4 cut(s) 76, 80, 451, 529
HpyCH4III ACNGT 4 cut(s) 28, 49, 491, 590
HpyCH4IV ACGT 2 cut(s) 162, 190
HpyCH4V TGCA 1 cut(s) 297
HpyF10VI GCNNNNNNNGC 4 cut(s) 95, 261, 264, 309
HpyF3I CTNAG 1 cut(s) 405
HpySE526I ACGT 2 cut(s) 162, 190
Hsp92II CATG 2 cut(s) 368, 562
HspAI GCGC 1 cut(s) 284
Kzo9I GATC 6 cut(s) 360, 369, 512, 538, 604, 632
LmnI GCTCC 2 cut(s) 152, 687
Lsp1109I GCAGC 3 cut(s) 234, 287, 309
LweI GCATC 1 cut(s) 367
MaeII ACGT 2 cut(s) 162, 190
MaeIII GTNAC 5 cut(s) 36, 320, 342, 494, 584
MalI GATC 6 cut(s) 362, 371, 514, 540, 606, 634
MboI GATC 6 cut(s) 360, 369, 512, 538, 604, 632
MboII GAAGA 5 cut(s) 16, 398, 541, 544, 654
MlyI GAGTC 3 cut(s) 350, 532, 590
MmeI TCCRAC 2 cut(s) 78, 174
MseI TTAA 1 cut(s) 170
MspA1I CMGCKG 1 cut(s) 300
MspI CCGG 2 cut(s) 281, 503
MspR9I CCNGG 3 cut(s) 128, 281, 504
MvaI CCWGG 1 cut(s) 128
MvnI CGCG 2 cut(s) 95, 286
MwoI GCNNNNNNNGC 4 cut(s) 95, 261, 264, 309
NciI CCSGG 2 cut(s) 281, 504
NdeII GATC 6 cut(s) 360, 369, 512, 538, 604, 632
NlaIII CATG 2 cut(s) 368, 562
NlaIV GGNNCC 3 cut(s) 275, 470, 689
NmuCI GTSAC 2 cut(s) 36, 342
NotI GCGGCCGC 1 cut(s) 96
NspV TTCGAA 1 cut(s) 384
PaeR7I CTCGAG 1 cut(s) 110
PcsI WCGNNNNNNNCGW 2 cut(s) 32, 381
PfeI GAWTC 1 cut(s) 349
PleI GAGTC 3 cut(s) 349, 531, 590
PpsI GAGTC 3 cut(s) 349, 531, 590
Ppu21I YACGTR 1 cut(s) 191
PpuMI RGGWCCY 1 cut(s) 124
Psp5II RGGWCCY 1 cut(s) 124
Psp6I CCWGG 1 cut(s) 126
PspFI CCCAGC 1 cut(s) 437
PspGI CCWGG 1 cut(s) 126
PspN4I GGNNCC 3 cut(s) 275, 470, 689
PspPI GGNCC 3 cut(s) 124, 274, 500
PspPPI RGGWCCY 1 cut(s) 124
PspXI VCTCGAGB 1 cut(s) 110
PstI CTGCAG 1 cut(s) 299
PvuII CAGCTG 1 cut(s) 300
RsaI GTAC 2 cut(s) 14, 592
RsaNI GTAC 2 cut(s) 13, 591
SaqAI TTAA 1 cut(s) 170
Sau3AI GATC 6 cut(s) 360, 369, 512, 538, 604, 632
Sau96I GGNCC 3 cut(s) 124, 274, 500
ScaI AGTACT 1 cut(s) 592
SchI GAGTC 3 cut(s) 350, 532, 590
ScrFI CCNGG 3 cut(s) 128, 281, 504
SfaNI GCATC 1 cut(s) 367
SfcI CTRYAG 1 cut(s) 295
Sfr274I CTCGAG 1 cut(s) 110
SfuI TTCGAA 1 cut(s) 384
SinI GGWCC 2 cut(s) 124, 274
SlaI CTCGAG 1 cut(s) 110
SmlI CTYRAG 1 cut(s) 110
SmoI CTYRAG 1 cut(s) 110
StyD4I CCNGG 3 cut(s) 126, 279, 502
TaaI ACNGT 4 cut(s) 28, 49, 491, 590
TaiI ACGT 2 cut(s) 165, 193
TaqI TCGA 5 cut(s) 111, 352, 375, 384, 443
TatI WGTACW 1 cut(s) 590
TauI GCSGC 7 cut(s) 98, 101, 245, 258, 267, 270, 437
TfiI GAWTC 1 cut(s) 349
Tru1I TTAA 1 cut(s) 170
Tru9I TTAA 1 cut(s) 170
TscAI CASTG 1 cut(s) 496
TseFI GTSAC 2 cut(s) 36, 342
TseI GCWGC 3 cut(s) 222, 297, 300
Tsp45I GTSAC 2 cut(s) 36, 342
TspDTI ATGAA 3 cut(s) 17, 20, 390
TspGWI ACGGA 1 cut(s) 24
TspRI CASTG 1 cut(s) 496
VpaK11BI GGWCC 2 cut(s) 124, 274
XhoI CTCGAG 1 cut(s) 110
ZrmI AGTACT 1 cut(s) 592
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.