MD11G1007900.v1.1

Cytokinin riboside 5'-monophosphate phosphoribohydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0001203
Physical Location & Seq
Forward (+)
695811 .. 700055
4245 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1007900.v1.1.491

Sequence Viewer

Length: 618 bp
ATGGGTAAGTTCAAAACGGTGTGTGTATTTTGTGGGAGTAATTCTGGGCACAGAAAGATATTCAGTGATGCAGCTCTTCAACTTGGGAAAGAACTGGTGGAGAGGAAGATGGATTTGGTATATGGAGGAGGAAGTGTAGGGTTGATGGGGTTGGTTTCTCAAACGGTTTATGATGGCGGATGTCATGTTCTTGGAGTAATCCCAAAAGCCCTAATTCCACTTGAGATATCAGGCAATTCAGTAGGACAAGTATTCGTTGTATCGAACATGCATGAAAGAAAGGCTGAGATGGCTCGTCGAGCTGATGCTTTCATTGCATTGCCCGGTGGGTATGGAACCATGGAAGAGTTGCTTGAGATGATAACATGGTCTCAGCTTGGAATCCATGATAAACCAGTGGGTCTTTTGAACATTGATGGGTATTATGATTGCTTGCTTCGTCTGTTTGATAAAGGTGTGGAAGAGGGATTTATCACTTTATCTGCTAGGAATATTGTCATATCTGCAAAAACTGCCACAGAGCTGATACAGAAGATGGAGGAATACATACCTTTGCATAATCAAGTGGCTCCAGCCCAAAACTGGAAAGTCGGAGAAAACCATGCAAATGATCTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lysine_decarbox

Protein Analysis

206

Amino Acids

22.38

Weight (kDa)

5.86

Isoelectric Point (pI)

35.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LDcluster4 PF18306 12 - 119 3.1e-12 SLOG cluster4 family
Lysine_decarbox PF03641 49 - 179 1.5e-45 Possible lysine decarboxylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 177
AfiI CCNNNNNNNGG 1 cut(s) 582
AgsI TTSAA 3 cut(s) 13, 80, 409
AjuI GAANNNNNNNTTGG 2 cut(s) 98, 130
AloI GAACNNNNNNTCC 2 cut(s) 171, 203
AluBI AGCT 4 cut(s) 74, 302, 376, 523
AluI AGCT 4 cut(s) 74, 302, 376, 523
Alw26I GTCTC 1 cut(s) 375
ApeKI GCWGC 1 cut(s) 71
AsuC2I CCSGG 1 cut(s) 324
BaeGI GKGCMC 1 cut(s) 51
BbvI GCAGC 1 cut(s) 83
BccI CCATC 6 cut(s) 103, 139, 167, 283, 410, 529
BcnI CCSGG 1 cut(s) 324
BcoDI GTCTC 1 cut(s) 375
BfaI CTAG 1 cut(s) 486
BisI GCNGC 1 cut(s) 72
BlsI GCNGC 1 cut(s) 73
Bme1390I CCNGG 1 cut(s) 324
BmiI GGNNCC 2 cut(s) 337, 570
BmrFI CCNGG 1 cut(s) 324
BmsI GCATC 2 cut(s) 58, 295
BpmI CTGGAG 1 cut(s) 555
BpuEI CTTGAG 2 cut(s) 242, 374
BpuMI CCSGG 1 cut(s) 324
BsaI GGTCTC 1 cut(s) 375
BsaJI CCNNGG 1 cut(s) 339
BsaXI ACNNNNNCTCC 2 cut(s) 120, 150
Bsc4I CCNNNNNNNGG 1 cut(s) 582
Bse1I ACTGG 3 cut(s) 99, 395, 587
Bse3DI GCAATG 2 cut(s) 312, 317
BseDI CCNNGG 1 cut(s) 339
BseGI GGATG 1 cut(s) 185
BseLI CCNNNNNNNGG 1 cut(s) 582
BseMI GCAATG 2 cut(s) 312, 317
BseMII CTCAG 2 cut(s) 276, 386
BseNI ACTGG 3 cut(s) 99, 395, 587
BseRI GAGGAG 1 cut(s) 141
BseSI GKGCMC 1 cut(s) 51
BseXI GCAGC 1 cut(s) 83
BsiSI CCGG 1 cut(s) 324
BslI CCNNNNNNNGG 1 cut(s) 582
BsmAI GTCTC 1 cut(s) 375
Bso31I GGTCTC 1 cut(s) 375
Bsp1286I GDGCHC 1 cut(s) 51
Bsp143I GATC 1 cut(s) 610
Bsp19I CCATGG 1 cut(s) 339
BspACI CCGC 1 cut(s) 177
BspCNI CTCAG 2 cut(s) 277, 385
BspLI GGNNCC 2 cut(s) 337, 570
BspQI GCTCTTC 1 cut(s) 81
BspTNI GGTCTC 1 cut(s) 375
BsrDI GCAATG 2 cut(s) 312, 317
BsrI ACTGG 3 cut(s) 99, 395, 587
BssECI CCNNGG 1 cut(s) 339
BssMI GATC 1 cut(s) 610
BssT1I CCWWGG 1 cut(s) 339
Bst4CI ACNGT 2 cut(s) 19, 166
Bst6I CTCTTC 3 cut(s) 81, 339, 456
BstAPI GCANNNNNTGC 1 cut(s) 512
BstC8I GCNNGC 1 cut(s) 434
BstDEI CTNAG 2 cut(s) 285, 372
BstDSI CCRYGG 1 cut(s) 339
BstF5I GGATG 1 cut(s) 185
BstKTI GATC 1 cut(s) 613
BstMAI GTCTC 1 cut(s) 375
BstMBI GATC 1 cut(s) 610
BstMWI GCNNNNNNNGC 4 cut(s) 290, 299, 314, 512
BstNSI RCATGY 1 cut(s) 271
BstSCI CCNGG 1 cut(s) 322
BstSLI GKGCMC 1 cut(s) 51
BstV1I GCAGC 1 cut(s) 83
BtgI CCRYGG 1 cut(s) 339
BtsCI GGATG 1 cut(s) 185
BtsIMutI CAGTG 2 cut(s) 70, 402
Cac8I GCNNGC 1 cut(s) 434
CviAII CATG 7 cut(s) 185, 268, 272, 340, 366, 386, 602
CviJI RGCY 9 cut(s) 74, 209, 284, 293, 302, 376, 523, 569, 575
CviKI_1 RGCY 9 cut(s) 74, 209, 284, 293, 302, 376, 523, 569, 575
DdeI CTNAG 2 cut(s) 285, 372
DpnI GATC 1 cut(s) 612
DpnII GATC 1 cut(s) 610
Eam1104I CTCTTC 3 cut(s) 81, 339, 456
EarI CTCTTC 3 cut(s) 81, 339, 456
EciI GGCGGA 1 cut(s) 192
Eco130I CCWWGG 1 cut(s) 339
Eco31I GGTCTC 1 cut(s) 375
Eco32I GATATC 1 cut(s) 228
EcoRV GATATC 1 cut(s) 228
EcoT14I CCWWGG 1 cut(s) 339
EcoT22I ATGCAT 1 cut(s) 273
ErhI CCWWGG 1 cut(s) 339
FaeI CATG 7 cut(s) 188, 271, 275, 343, 369, 389, 605
FalI AAGNNNNNCTT 2 cut(s) 336, 368
FatI CATG 7 cut(s) 184, 267, 271, 339, 365, 385, 601
Fnu4HI GCNGC 1 cut(s) 72
FokI GGATG 1 cut(s) 192
Fsp4HI GCNGC 1 cut(s) 72
FspBI CTAG 1 cut(s) 486
GluI GCNGC 1 cut(s) 72
GsuI CTGGAG 1 cut(s) 555
HapII CCGG 1 cut(s) 324
Hin1II CATG 7 cut(s) 188, 271, 275, 343, 369, 389, 605
HinfI GANTC 1 cut(s) 381
HpaII CCGG 1 cut(s) 324
Hpy188I TCNGA 1 cut(s) 593
Hpy99I CGWCG 1 cut(s) 300
HpyCH4III ACNGT 2 cut(s) 19, 166
HpyCH4V TGCA 6 cut(s) 71, 271, 317, 506, 556, 605
HpyF10VI GCNNNNNNNGC 4 cut(s) 290, 299, 314, 512
HpyF3I CTNAG 2 cut(s) 285, 372
Hsp92II CATG 7 cut(s) 188, 271, 275, 343, 369, 389, 605
Kzo9I GATC 1 cut(s) 610
LguI GCTCTTC 1 cut(s) 81
LmnI GCTCC 1 cut(s) 574
LpnPI CCDG 7 cut(s) 30, 80, 216, 337, 408, 568, 585
Lsp1109I GCAGC 1 cut(s) 83
LweI GCATC 2 cut(s) 58, 295
MaeI CTAG 1 cut(s) 486
MalI GATC 1 cut(s) 612
MboI GATC 1 cut(s) 610
MboII GAAGA 5 cut(s) 68, 118, 356, 473, 544
MhlI GDGCHC 1 cut(s) 51
MluCI AATT 3 cut(s) 40, 213, 235
MmeI TCCRAC 1 cut(s) 571
MnlI CCTC 5 cut(s) 96, 119, 122, 457, 532
Mph1103I ATGCAT 1 cut(s) 273
MslI CAYNNNNRTG 1 cut(s) 606
MspI CCGG 1 cut(s) 324
MspR9I CCNGG 1 cut(s) 324
MwoI GCNNNNNNNGC 4 cut(s) 290, 299, 314, 512
NciI CCSGG 1 cut(s) 324
NcoI CCATGG 1 cut(s) 339
NdeII GATC 1 cut(s) 610
NlaIII CATG 7 cut(s) 188, 271, 275, 343, 369, 389, 605
NlaIV GGNNCC 2 cut(s) 337, 570
NsiI ATGCAT 1 cut(s) 273
NspI RCATGY 1 cut(s) 271
PciSI GCTCTTC 1 cut(s) 81
PfeI GAWTC 1 cut(s) 381
PkrI GCNGC 1 cut(s) 73
PspN4I GGNNCC 2 cut(s) 337, 570
RseI CAYNNNNRTG 1 cut(s) 606
SapI GCTCTTC 1 cut(s) 81
SatI GCNGC 1 cut(s) 72
Sau3AI GATC 1 cut(s) 610
ScrFI CCNGG 1 cut(s) 324
SduI GDGCHC 1 cut(s) 51
SetI ASST 6 cut(s) 76, 304, 378, 457, 525, 553
SfaNI GCATC 2 cut(s) 58, 295
SmiMI CAYNNNNRTG 1 cut(s) 606
SmlI CTYRAG 2 cut(s) 221, 353
SmoI CTYRAG 2 cut(s) 221, 353
Sse9I AATT 3 cut(s) 40, 213, 235
SsiI CCGC 1 cut(s) 177
SspI AATATT 1 cut(s) 493
SspMI CTAG 1 cut(s) 486
StyD4I CCNGG 1 cut(s) 322
StyI CCWWGG 1 cut(s) 339
TaaI ACNGT 2 cut(s) 19, 166
TaqI TCGA 2 cut(s) 263, 298
TasI AATT 3 cut(s) 40, 213, 235
TfiI GAWTC 1 cut(s) 381
TscAI CASTG 2 cut(s) 70, 402
TseI GCWGC 1 cut(s) 71
TspDTI ATGAA 2 cut(s) 288, 301
TspRI CASTG 2 cut(s) 70, 402
XceI RCATGY 1 cut(s) 271
XcmI CCANNNNNNNNNTGG 1 cut(s) 579
XspI CTAG 1 cut(s) 486
Zsp2I ATGCAT 1 cut(s) 273
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.