Rh6CG441200
NAC Family

Cytokinin-activating enzyme working in the direct activation pathway. Phosphoribohydrolase that converts inactive cytokinin nucleotides to the biologically active free-base forms

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0001203
Physical Location & Seq
Forward (+)
62092966 .. 62095360
2395 bp
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UTR
Exon/CDS
Intron
Rh6CG441200.1

Sequence Viewer

Length: 630 bp
ATGGAGATGATTCAAGAACAACAACAACTAGAGGGTGTAATGAAATCTTCAAGATTCAAGCGTATCTGTGTTTTCTGTGGTAGCAGTGCTGGAAAGAACCCAAGTTATCAGCTTGCTGCTGTTCAACTGGGAAAACAACTGGTTGAAAGGAACATTGACTTGGTTTATGGAGGAGGGAGCATTGGTTTGATGGGTCAGGTCTCCCAAGCTGTTTATGATGGTGGTCGCCACGTGTTGGGAGTAATTCCCAGAACTCTCATGCCAAGAGAGATCACTGGAGAGCCTGTGGGAGAAGTTAGAGCTGTATCTGGTATGCACCAGCGCAAAGCGGAAATGGCTCGCCAAGCTGATGCCTTTGTTGCCTTGCCAGGTGGCTATGGTACCTTAGAAGAACTCTTGGAAGTGATCACTTGGGCTCAGTTGGGAATTCATGAGAAACCAGTGGGGTTGTTAAATGTTGATGGGTACTACAACTCACTTTTAACCTTCATAGACAACGCTGTGGATGAAGGTTTCATTGACCCTGCAGCTAGGCACATTATTGTGTCTGCCCCAACTGCTCAGGAACTCATGTGCAAGCTAGAGGTACATTACATTCCCCTCTTTACATGGTATAATGATGATCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lysine_decarbox

Protein Analysis

209

Amino Acids

23.0

Weight (kDa)

5.22

Isoelectric Point (pI)

36.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LDcluster4 PF18306 27 - 152 1.5e-13 SLOG cluster4 family
Lysine_decarbox PF03641 64 - 193 3.3e-48 Possible lysine decarboxylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 380
AccB1I GGYRCC 1 cut(s) 380
AccB7I CCANNNNNTGG 1 cut(s) 235
AciI CCGC 1 cut(s) 329
AcsI RAATTY 1 cut(s) 426
AcvI CACGTG 1 cut(s) 232
AfaI GTAC 3 cut(s) 382, 467, 588
AfiI CCNNNNNNNGG 1 cut(s) 235
AflIII ACRYGT 1 cut(s) 231
AgsI TTSAA 5 cut(s) 14, 51, 58, 125, 146
AjnI CCWGG 1 cut(s) 367
AjuI GAANNNNNNNTTGG 2 cut(s) 143, 175
AluBI AGCT 6 cut(s) 112, 209, 302, 347, 530, 580
AluI AGCT 6 cut(s) 112, 209, 302, 347, 530, 580
Alw26I GTCTC 1 cut(s) 205
ApeKI GCWGC 2 cut(s) 116, 527
ApoI RAATTY 1 cut(s) 426
Asp718I GGTACC 1 cut(s) 380
AspLEI GCGC 1 cut(s) 324
BanI GGYRCC 1 cut(s) 380
BanII GRGCYC 1 cut(s) 418
BbrPI CACGTG 1 cut(s) 232
BbvI GCAGC 2 cut(s) 103, 539
BccI CCATC 3 cut(s) 184, 212, 455
BciT130I CCWGG 1 cut(s) 369
BclI TGATCA 2 cut(s) 405, 622
BcoDI GTCTC 1 cut(s) 205
BfaI CTAG 3 cut(s) 29, 531, 581
BfmI CTRYAG 1 cut(s) 525
BisI GCNGC 2 cut(s) 117, 528
BlsI GCNGC 2 cut(s) 118, 529
Bme1390I CCNGG 1 cut(s) 369
BmiI GGNNCC 1 cut(s) 382
BmrFI CCNGG 1 cut(s) 369
BmrI ACTGGG 1 cut(s) 137
BmsI GCATC 1 cut(s) 340
BmuI ACTGGG 1 cut(s) 137
BpmI CTGGAG 1 cut(s) 297
Bpu10I CCTNAGC 1 cut(s) 561
BsaAI YACGTR 1 cut(s) 232
BsaI GGTCTC 1 cut(s) 205
BsaXI ACNNNNNCTCC 4 cut(s) 169, 199, 270, 300
Bsc4I CCNNNNNNNGG 1 cut(s) 235
Bse1I ACTGG 4 cut(s) 132, 144, 280, 440
BseBI CCWGG 1 cut(s) 369
BseGI GGATG 1 cut(s) 511
BseLI CCNNNNNNNGG 1 cut(s) 235
BseMII CTCAG 2 cut(s) 431, 575
BseNI ACTGG 4 cut(s) 132, 144, 280, 440
BseRI GAGGAG 1 cut(s) 186
BseXI GCAGC 2 cut(s) 103, 539
BshNI GGYRCC 1 cut(s) 380
BslI CCNNNNNNNGG 1 cut(s) 235
BsmAI GTCTC 1 cut(s) 205
Bso31I GGTCTC 1 cut(s) 205
Bsp1286I GDGCHC 1 cut(s) 418
Bsp143I GATC 3 cut(s) 270, 405, 622
BspACI CCGC 1 cut(s) 329
BspCNI CTCAG 2 cut(s) 430, 574
BspHI TCATGA 1 cut(s) 430
BspLI GGNNCC 1 cut(s) 382
BspMAI CTGCAG 1 cut(s) 529
BspT107I GGYRCC 1 cut(s) 380
BspTNI GGTCTC 1 cut(s) 205
BsrI ACTGG 4 cut(s) 132, 144, 280, 440
BssMI GATC 3 cut(s) 270, 405, 622
Bst2UI CCWGG 1 cut(s) 369
BstBAI YACGTR 1 cut(s) 232
BstC8I GCNNGC 3 cut(s) 114, 340, 578
BstDEI CTNAG 3 cut(s) 385, 417, 561
BstF5I GGATG 1 cut(s) 511
BstHHI GCGC 1 cut(s) 324
BstKTI GATC 3 cut(s) 273, 408, 625
BstMAI GTCTC 1 cut(s) 205
BstMBI GATC 3 cut(s) 270, 405, 622
BstMWI GCNNNNNNNGC 4 cut(s) 335, 344, 359, 557
BstNI CCWGG 1 cut(s) 369
BstSCI CCNGG 1 cut(s) 367
BstSFI CTRYAG 1 cut(s) 525
BstV1I GCAGC 2 cut(s) 103, 539
BtsCI GGATG 1 cut(s) 511
BtsI GCAGTG 1 cut(s) 91
BtsIMutI CAGTG 3 cut(s) 91, 273, 447
Cac8I GCNNGC 3 cut(s) 114, 340, 578
CciI TCATGA 1 cut(s) 430
CfoI GCGC 1 cut(s) 324
Csp6I GTAC 3 cut(s) 381, 466, 587
CviAII CATG 4 cut(s) 259, 431, 571, 609
CviQI GTAC 3 cut(s) 381, 466, 587
DdeI CTNAG 3 cut(s) 385, 417, 561
DpnI GATC 3 cut(s) 272, 407, 624
DpnII GATC 3 cut(s) 270, 405, 622
Eco24I GRGCYC 1 cut(s) 418
Eco31I GGTCTC 1 cut(s) 205
Eco72I CACGTG 1 cut(s) 232
EcoRI GAATTC 1 cut(s) 426
EcoRII CCWGG 1 cut(s) 367
EcoT38I GRGCYC 1 cut(s) 418
FaeI CATG 4 cut(s) 262, 434, 574, 612
FatI CATG 4 cut(s) 258, 430, 570, 608
FbaI TGATCA 2 cut(s) 405, 622
Fnu4HI GCNGC 2 cut(s) 117, 528
FokI GGATG 1 cut(s) 518
FriOI GRGCYC 1 cut(s) 418
Fsp4HI GCNGC 2 cut(s) 117, 528
FspBI CTAG 3 cut(s) 29, 531, 581
GlaI GCGC 1 cut(s) 323
GluI GCNGC 2 cut(s) 117, 528
GsuI CTGGAG 1 cut(s) 297
HhaI GCGC 1 cut(s) 324
Hin1II CATG 4 cut(s) 262, 434, 574, 612
Hin6I GCGC 1 cut(s) 322
HinP1I GCGC 1 cut(s) 322
HinfI GANTC 2 cut(s) 10, 54
Hpy188III TCNNGA 4 cut(s) 14, 51, 431, 563
HpyAV CCTTC 2 cut(s) 496, 503
HpyCH4IV ACGT 1 cut(s) 231
HpyCH4V TGCA 3 cut(s) 316, 527, 576
HpyF10VI GCNNNNNNNGC 4 cut(s) 335, 344, 359, 557
HpyF3I CTNAG 3 cut(s) 385, 417, 561
HpySE526I ACGT 1 cut(s) 231
Hsp92II CATG 4 cut(s) 262, 434, 574, 612
HspAI GCGC 1 cut(s) 322
KpnI GGTACC 1 cut(s) 384
Ksp22I TGATCA 2 cut(s) 405, 622
Kzo9I GATC 3 cut(s) 270, 405, 622
LmnI GCTCC 1 cut(s) 177
Lsp1109I GCAGC 2 cut(s) 103, 539
LweI GCATC 1 cut(s) 340
MaeI CTAG 3 cut(s) 29, 531, 581
MaeII ACGT 1 cut(s) 231
MalI GATC 3 cut(s) 272, 407, 624
MboI GATC 3 cut(s) 270, 405, 622
MboII GAAGA 2 cut(s) 39, 401
MhlI GDGCHC 1 cut(s) 418
MluCI AATT 2 cut(s) 243, 426
MnlI CCTC 5 cut(s) 25, 164, 167, 577, 611
MseI TTAA 2 cut(s) 452, 482
MslI CAYNNNNRTG 1 cut(s) 542
MspR9I CCNGG 1 cut(s) 369
MvaI CCWGG 1 cut(s) 369
MwoI GCNNNNNNNGC 4 cut(s) 335, 344, 359, 557
NdeII GATC 3 cut(s) 270, 405, 622
NlaIII CATG 4 cut(s) 262, 434, 574, 612
NlaIV GGNNCC 1 cut(s) 382
PagI TCATGA 1 cut(s) 430
PfeI GAWTC 2 cut(s) 10, 54
PflMI CCANNNNNTGG 1 cut(s) 235
PkrI GCNGC 2 cut(s) 118, 529
PmaCI CACGTG 1 cut(s) 232
PmlI CACGTG 1 cut(s) 232
Ppu21I YACGTR 1 cut(s) 232
Psp6I CCWGG 1 cut(s) 367
PspCI CACGTG 1 cut(s) 232
PspGI CCWGG 1 cut(s) 367
PspN4I GGNNCC 1 cut(s) 382
PstI CTGCAG 1 cut(s) 529
RsaI GTAC 3 cut(s) 382, 467, 588
RsaNI GTAC 3 cut(s) 381, 466, 587
RseI CAYNNNNRTG 1 cut(s) 542
SaqAI TTAA 2 cut(s) 452, 482
SatI GCNGC 2 cut(s) 117, 528
Sau3AI GATC 3 cut(s) 270, 405, 622
ScrFI CCNGG 1 cut(s) 369
SduI GDGCHC 1 cut(s) 418
SfaNI GCATC 1 cut(s) 340
SfcI CTRYAG 1 cut(s) 525
SmiMI CAYNNNNRTG 1 cut(s) 542
Sse9I AATT 2 cut(s) 243, 426
SsiI CCGC 1 cut(s) 329
SspMI CTAG 3 cut(s) 29, 531, 581
StyD4I CCNGG 1 cut(s) 367
TaiI ACGT 1 cut(s) 234
TasI AATT 2 cut(s) 243, 426
TfiI GAWTC 2 cut(s) 10, 54
Tru1I TTAA 2 cut(s) 452, 482
Tru9I TTAA 2 cut(s) 452, 482
TscAI CASTG 3 cut(s) 91, 280, 447
TseI GCWGC 2 cut(s) 116, 527
TspDTI ATGAA 5 cut(s) 56, 419, 478, 505, 522
TspRI CASTG 3 cut(s) 91, 280, 447
Van91I CCANNNNNTGG 1 cut(s) 235
XapI RAATTY 1 cut(s) 426
XspI CTAG 3 cut(s) 29, 531, 581
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.