MD15G1013800.v1.1
NAC Family

Cytokinin-activating enzyme working in the direct activation pathway. Phosphoribohydrolase that converts inactive cytokinin nucleotides to the biologically active free-base forms

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0001203
Physical Location & Seq
Reverse (-)
752561 .. 755149
2589 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1013800.v1.1.491

Sequence Viewer

Length: 645 bp
ATGGAACATCAGCAGCAGACTGCAATGAAATCGAGATTCAGCCGTATCTGCGTTTTCTGTGGCAGCAGTGCTGGCAAGAACCCGAGCTACCAGCTCTCTGCCATTAAACTCGGCAAAGAATTGGTTGAAAGGAGCATCGACTTGGTTTACGGAGGAGGAAGCATTGGATTGATGGGTCAGGTCTCCCAAGTTGTCTATGATGGTGGCCGCCACGTGTTGGGAGTAATTCCCACAACTCTCATGCCAAGAGAGATCACAGGAGAGCCAGTGGGAGAAGTAAGAGCTGTATCAAGCATGCACCAACGCAAAGCAGAAATGGCTCGCCAAGCTGATGCCTTTATTGCCTTGCCAGGTGGCTATGGTACCTTGGAGGAACTGTTGGAAGTGATCACTTGGGCACAGTTGGGAATCCATGACAAACCGGTGGGGTTGTTAAATGTGGATGGATACTACAACTCACTGCTATCCTTCATAGACAAGGCTGTGGATGAAGGTTTCATTGACCCTGCAGCTCGACATATTATTGTCTCTGCCGAAACTCCCCACGAACTCATGTGCAAAGAATATGCTCCAAAGCATTGTGGACTGAGTTGGGAGATGGAGCAACAACTGGAAAACACGGCAAAGTCAGACATTGCACGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lysine_decarbox

Protein Analysis

215

Amino Acids

23.43

Weight (kDa)

5.64

Isoelectric Point (pI)

30.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LDcluster4 PF18306 21 - 146 2.6e-11 SLOG cluster4 family
Lysine_decarbox PF03641 58 - 185 3.8e-47 Possible lysine decarboxylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 362
AccB1I GGYRCC 1 cut(s) 362
AccB7I CCANNNNNTGG 1 cut(s) 217
AciI CCGC 1 cut(s) 208
AcoI YGGCCR 1 cut(s) 205
AcvI CACGTG 1 cut(s) 214
AfaI GTAC 1 cut(s) 364
AfiI CCNNNNNNNGG 1 cut(s) 217
AflIII ACRYGT 1 cut(s) 213
AgeI ACCGGT 1 cut(s) 421
AgsI TTSAA 1 cut(s) 128
AjnI CCWGG 1 cut(s) 349
AluBI AGCT 5 cut(s) 87, 94, 284, 329, 512
AluI AGCT 5 cut(s) 87, 94, 284, 329, 512
Alw26I GTCTC 2 cut(s) 187, 532
Ama87I CYCGRG 1 cut(s) 82
AoxI GGCC 1 cut(s) 205
ApeKI GCWGC 3 cut(s) 13, 63, 509
AsiGI ACCGGT 1 cut(s) 421
Asp718I GGTACC 1 cut(s) 362
AvaI CYCGRG 1 cut(s) 82
BaeGI GKGCMC 1 cut(s) 400
BanI GGYRCC 1 cut(s) 362
BbrPI CACGTG 1 cut(s) 214
BbvI GCAGC 3 cut(s) 25, 75, 521
BccI CCATC 4 cut(s) 166, 194, 437, 592
BceAI ACGGC 2 cut(s) 27, 636
BcgI CGANNNNNNTGC 2 cut(s) 12, 46
BciT130I CCWGG 1 cut(s) 351
BciVI GTATCC 1 cut(s) 440
BclI TGATCA 1 cut(s) 387
BcoDI GTCTC 2 cut(s) 187, 532
BfmI CTRYAG 1 cut(s) 507
BfuI GTATCC 1 cut(s) 440
BisI GCNGC 4 cut(s) 14, 64, 208, 510
BlsI GCNGC 4 cut(s) 15, 65, 209, 511
Bme1390I CCNGG 1 cut(s) 351
BmeT110I CYCGRG 1 cut(s) 82
BmiI GGNNCC 1 cut(s) 364
BmrFI CCNGG 1 cut(s) 351
BmsI GCATC 2 cut(s) 144, 322
BsaAI YACGTR 1 cut(s) 214
BsaI GGTCTC 1 cut(s) 187
BsaJI CCNNGG 1 cut(s) 366
BsaWI WCCGGW 1 cut(s) 421
BsaXI ACNNNNNCTCC 4 cut(s) 252, 282, 362, 392
Bsc4I CCNNNNNNNGG 1 cut(s) 217
Bse118I RCCGGY 1 cut(s) 421
Bse1I ACTGG 2 cut(s) 266, 615
Bse3DI GCAATG 2 cut(s) 30, 633
BseBI CCWGG 1 cut(s) 351
BseDI CCNNGG 1 cut(s) 366
BseGI GGATG 2 cut(s) 448, 493
BseLI CCNNNNNNNGG 1 cut(s) 217
BseMI GCAATG 2 cut(s) 30, 633
BseMII CTCAG 1 cut(s) 578
BseNI ACTGG 2 cut(s) 266, 615
BseRI GAGGAG 1 cut(s) 168
BseSI GKGCMC 1 cut(s) 400
BseXI GCAGC 3 cut(s) 25, 75, 521
BshFI GGCC 1 cut(s) 207
BshNI GGYRCC 1 cut(s) 362
BshTI ACCGGT 1 cut(s) 421
BsiHKCI CYCGRG 1 cut(s) 82
BsiSI CCGG 1 cut(s) 422
BslI CCNNNNNNNGG 1 cut(s) 217
BsmAI GTCTC 2 cut(s) 187, 532
BsnI GGCC 1 cut(s) 207
Bso31I GGTCTC 1 cut(s) 187
BsoBI CYCGRG 1 cut(s) 82
Bsp1286I GDGCHC 1 cut(s) 400
Bsp143I GATC 2 cut(s) 252, 387
BspACI CCGC 1 cut(s) 208
BspANI GGCC 1 cut(s) 207
BspCNI CTCAG 1 cut(s) 579
BspLI GGNNCC 1 cut(s) 364
BspMAI CTGCAG 1 cut(s) 511
BspT107I GGYRCC 1 cut(s) 362
BspTNI GGTCTC 1 cut(s) 187
BsrDI GCAATG 2 cut(s) 30, 633
BsrFI RCCGGY 1 cut(s) 421
BsrI ACTGG 2 cut(s) 266, 615
BssAI RCCGGY 1 cut(s) 421
BssECI CCNNGG 1 cut(s) 366
BssMI GATC 2 cut(s) 252, 387
BssT1I CCWWGG 1 cut(s) 366
Bst2UI CCWGG 1 cut(s) 351
Bst4CI ACNGT 2 cut(s) 378, 402
BstBAI YACGTR 1 cut(s) 214
BstC8I GCNNGC 3 cut(s) 73, 296, 322
BstDEI CTNAG 1 cut(s) 587
BstF5I GGATG 2 cut(s) 448, 493
BstKTI GATC 2 cut(s) 255, 390
BstMAI GTCTC 2 cut(s) 187, 532
BstMBI GATC 2 cut(s) 252, 387
BstMWI GCNNNNNNNGC 5 cut(s) 48, 72, 317, 326, 341
BstNI CCWGG 1 cut(s) 351
BstNSI RCATGY 1 cut(s) 298
BstSCI CCNGG 1 cut(s) 349
BstSFI CTRYAG 1 cut(s) 507
BstSLI GKGCMC 1 cut(s) 400
BstV1I GCAGC 3 cut(s) 25, 75, 521
BsuI GTATCC 1 cut(s) 440
BsuRI GGCC 1 cut(s) 207
BtsCI GGATG 2 cut(s) 448, 493
BtsI GCAGTG 2 cut(s) 73, 458
BtsIMutI CAGTG 3 cut(s) 73, 273, 458
Cac8I GCNNGC 3 cut(s) 73, 296, 322
Cfr10I RCCGGY 1 cut(s) 421
Csp6I GTAC 1 cut(s) 363
CspAI ACCGGT 1 cut(s) 421
CviAII CATG 4 cut(s) 241, 295, 413, 553
CviQI GTAC 1 cut(s) 363
DdeI CTNAG 1 cut(s) 587
DpnI GATC 2 cut(s) 254, 389
DpnII GATC 2 cut(s) 252, 387
EaeI YGGCCR 1 cut(s) 205
Eco130I CCWWGG 1 cut(s) 366
Eco31I GGTCTC 1 cut(s) 187
Eco72I CACGTG 1 cut(s) 214
Eco88I CYCGRG 1 cut(s) 82
EcoRII CCWGG 1 cut(s) 349
EcoT14I CCWWGG 1 cut(s) 366
ErhI CCWWGG 1 cut(s) 366
FaeI CATG 4 cut(s) 244, 298, 416, 556
FaiI YATR 9 cut(s) 198, 242, 296, 360, 414, 473, 519, 554, 567
FatI CATG 4 cut(s) 240, 294, 412, 552
FbaI TGATCA 1 cut(s) 387
Fnu4HI GCNGC 4 cut(s) 14, 64, 208, 510
FokI GGATG 2 cut(s) 455, 500
Fsp4HI GCNGC 4 cut(s) 14, 64, 208, 510
GluI GCNGC 4 cut(s) 14, 64, 208, 510
HaeIII GGCC 1 cut(s) 207
HapII CCGG 1 cut(s) 422
Hin1II CATG 4 cut(s) 244, 298, 416, 556
HinfI GANTC 2 cut(s) 36, 408
HpaII CCGG 1 cut(s) 422
Hpy166II GTNNAC 2 cut(s) 148, 584
Hpy188I TCNGA 1 cut(s) 631
Hpy188III TCNNGA 1 cut(s) 33
Hpy8I GTNNAC 2 cut(s) 148, 584
HpyAV CCTTC 2 cut(s) 478, 485
HpyCH4III ACNGT 2 cut(s) 378, 402
HpyCH4IV ACGT 2 cut(s) 213, 640
HpyCH4V TGCA 5 cut(s) 23, 298, 509, 558, 638
HpyF10VI GCNNNNNNNGC 5 cut(s) 48, 72, 317, 326, 341
HpyF3I CTNAG 1 cut(s) 587
HpySE526I ACGT 2 cut(s) 213, 640
Hsp92II CATG 4 cut(s) 244, 298, 416, 556
KpnI GGTACC 1 cut(s) 366
Ksp22I TGATCA 1 cut(s) 387
Kzo9I GATC 2 cut(s) 252, 387
LmnI GCTCC 3 cut(s) 132, 574, 601
Lsp1109I GCAGC 3 cut(s) 25, 75, 521
LweI GCATC 2 cut(s) 144, 322
MaeII ACGT 2 cut(s) 213, 640
MalI GATC 2 cut(s) 254, 389
MboI GATC 2 cut(s) 252, 387
MhlI GDGCHC 1 cut(s) 400
MluCI AATT 2 cut(s) 119, 225
MmeI TCCRAC 1 cut(s) 360
MnlI CCTC 3 cut(s) 146, 149, 364
MseI TTAA 2 cut(s) 105, 434
MspI CCGG 1 cut(s) 422
MspR9I CCNGG 1 cut(s) 351
MvaI CCWGG 1 cut(s) 351
MwoI GCNNNNNNNGC 5 cut(s) 48, 72, 317, 326, 341
NdeII GATC 2 cut(s) 252, 387
NlaIII CATG 4 cut(s) 244, 298, 416, 556
NlaIV GGNNCC 1 cut(s) 364
NmeAIII GCCGAG 1 cut(s) 90
NspI RCATGY 1 cut(s) 298
PaeI GCATGC 1 cut(s) 298
PfeI GAWTC 2 cut(s) 36, 408
PflMI CCANNNNNTGG 1 cut(s) 217
PinAI ACCGGT 1 cut(s) 421
PkrI GCNGC 4 cut(s) 15, 65, 209, 511
PmaCI CACGTG 1 cut(s) 214
PmlI CACGTG 1 cut(s) 214
Ppu21I YACGTR 1 cut(s) 214
Psp6I CCWGG 1 cut(s) 349
PspCI CACGTG 1 cut(s) 214
PspGI CCWGG 1 cut(s) 349
PspN4I GGNNCC 1 cut(s) 364
PsrI GAACNNNNNNTAC 2 cut(s) 71, 103
PstI CTGCAG 1 cut(s) 511
RsaI GTAC 1 cut(s) 364
RsaNI GTAC 1 cut(s) 363
SaqAI TTAA 2 cut(s) 105, 434
SatI GCNGC 4 cut(s) 14, 64, 208, 510
Sau3AI GATC 2 cut(s) 252, 387
ScrFI CCNGG 1 cut(s) 351
SduI GDGCHC 1 cut(s) 400
SfaNI GCATC 2 cut(s) 144, 322
SfcI CTRYAG 1 cut(s) 507
SphI GCATGC 1 cut(s) 298
Sse9I AATT 2 cut(s) 119, 225
SsiI CCGC 1 cut(s) 208
StyD4I CCNGG 1 cut(s) 349
StyI CCWWGG 1 cut(s) 366
TaaI ACNGT 2 cut(s) 378, 402
TaiI ACGT 2 cut(s) 216, 643
TaqI TCGA 3 cut(s) 32, 138, 514
TasI AATT 2 cut(s) 119, 225
TauI GCSGC 1 cut(s) 210
TfiI GAWTC 2 cut(s) 36, 408
Tru1I TTAA 2 cut(s) 105, 434
Tru9I TTAA 2 cut(s) 105, 434
TscAI CASTG 3 cut(s) 73, 273, 465
TseI GCWGC 3 cut(s) 13, 63, 509
TspDTI ATGAA 4 cut(s) 41, 460, 487, 504
TspGWI ACGGA 1 cut(s) 165
TspRI CASTG 3 cut(s) 73, 273, 465
Van91I CCANNNNNTGG 1 cut(s) 217
XceI RCATGY 1 cut(s) 298
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.