pycom08g05310
NAC Family

Cytokinin-activating enzyme working in the direct activation pathway. Phosphoribohydrolase that converts inactive cytokinin nucleotides to the biologically active free-base forms

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0001203
Physical Location & Seq
Forward (+)
4093113 .. 4094996
1884 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g05310.3

Sequence Viewer

Length: 678 bp
ATGGAAGAGAGCAAAAGCAGCAAGTTCAGGAGGATTTGTGTGTTCTGTGGAAGCAACTCTGGCCGCAGAAAAGTGTTCAGCGATGCTACTCTGGAGTTGGGTAATGAACTGGTGAAAAGGAAGATAGATTTGGTGTATGGCGGGGGAAGTGTCGGGTTGATGGGTTTGATATCGCACACAGTGTATGAAGGAGGTTGCCATGTTCTTGGGGTCATTCCAAAAGCTCTCATGCCTCTTGAGATATCTGGTCCATCCGTTGGAGAAGTAAGAACAGTTGCAAACATGCATGAACGCAAAGCTTTGATGGCTCAAGCGGCTGATGCCTTCATTGCTCTTCCTGGAGGATATGGAACTATGGAAGAGCTGTTGGAGATAATAACATGGGCGCAACTTGGGATTCACATGAAGCCGGTTGGTTTGCTTAATGTAGATGGGTACTATAATACCTTGCTTGCGCTATTTGACAATGGTGTCGAAGAAGGATTCATCAAGCCAGGTGCTCGGCATATAGTTGTCTCTGCTCCAACAGCCAAAGAACTTGTTCAGAAGATGGAGCAATACATTCCTTCCCATGACCATGTTGCTTCTCATGAAAGCTGGAAAAAGGAGCAACTCGGTAAAAGTCAAGCCAAGAACACGCAAGGTGAAACTGTTACCGGCAATAATTCTTTCACCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lysine_decarbox

Protein Analysis

226

Amino Acids

24.43

Weight (kDa)

6.71

Isoelectric Point (pI)

31.52

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 470
AccB7I CCANNNNNTGG 1 cut(s) 257
AciI CCGC 3 cut(s) 64, 141, 314
AcoI YGGCCR 1 cut(s) 61
AdeI CACNNNGTG 1 cut(s) 181
AfaI GTAC 1 cut(s) 437
AfiI CCNNNNNNNGG 1 cut(s) 257
AjnI CCWGG 2 cut(s) 337, 493
AjuI GAANNNNNNNTTGG 2 cut(s) 113, 145
AluBI AGCT 4 cut(s) 224, 299, 364, 597
AluI AGCT 4 cut(s) 224, 299, 364, 597
Alw21I GWGCWC 1 cut(s) 502
Alw26I GTCTC 1 cut(s) 520
AoxI GGCC 1 cut(s) 61
ApeKI GCWGC 1 cut(s) 18
Asp700I GAANNNNTTC 1 cut(s) 540
AspLEI GCGC 2 cut(s) 388, 457
AspS9I GGNCC 1 cut(s) 248
AsuHPI GGTGA 3 cut(s) 124, 656, 664
AvaII GGWCC 1 cut(s) 248
Bbv12I GWGCWC 1 cut(s) 502
BbvI GCAGC 1 cut(s) 30
BccI CCATC 5 cut(s) 154, 259, 298, 425, 544
BciT130I CCWGG 2 cut(s) 339, 495
BcoDI GTCTC 1 cut(s) 520
BfaI CTAG 1 cut(s) 676
BisI GCNGC 3 cut(s) 19, 64, 315
BlsI GCNGC 3 cut(s) 20, 65, 316
Bme1390I CCNGG 2 cut(s) 339, 495
Bme18I GGWCC 1 cut(s) 248
BmgT120I GGNCC 1 cut(s) 248
BmrFI CCNGG 2 cut(s) 339, 495
BmsI GCATC 2 cut(s) 73, 310
BpmI CTGGAG 2 cut(s) 113, 360
BpuEI CTTGAG 2 cut(s) 257, 294
BsaXI ACNNNNNCTCC 2 cut(s) 22, 52
Bsc4I CCNNNNNNNGG 1 cut(s) 257
Bse118I RCCGGY 2 cut(s) 409, 656
Bse1I ACTGG 1 cut(s) 114
Bse3DI GCAATG 1 cut(s) 327
BseBI CCWGG 2 cut(s) 339, 495
BseGI GGATG 1 cut(s) 251
BseLI CCNNNNNNNGG 1 cut(s) 257
BseMI GCAATG 1 cut(s) 327
BseNI ACTGG 1 cut(s) 114
BseXI GCAGC 1 cut(s) 30
BshFI GGCC 1 cut(s) 63
BsiHKAI GWGCWC 1 cut(s) 502
BsiSI CCGG 2 cut(s) 410, 657
BslI CCNNNNNNNGG 1 cut(s) 257
BsmAI GTCTC 1 cut(s) 520
BsnI GGCC 1 cut(s) 63
Bsp1286I GDGCHC 1 cut(s) 502
BspACI CCGC 3 cut(s) 64, 141, 314
BspANI GGCC 1 cut(s) 63
BspHI TCATGA 1 cut(s) 589
BspQI GCTCTTC 2 cut(s) 339, 354
BsrDI GCAATG 1 cut(s) 327
BsrFI RCCGGY 2 cut(s) 409, 656
BsrI ACTGG 1 cut(s) 114
BssAI RCCGGY 2 cut(s) 409, 656
Bst2UI CCWGG 2 cut(s) 339, 495
Bst4CI ACNGT 3 cut(s) 181, 274, 652
Bst6I CTCTTC 2 cut(s) 339, 354
BstC8I GCNNGC 1 cut(s) 453
BstF5I GGATG 1 cut(s) 251
BstHHI GCGC 2 cut(s) 388, 457
BstMAI GTCTC 1 cut(s) 520
BstMWI GCNNNNNNNGC 7 cut(s) 18, 60, 305, 314, 320, 329, 527
BstNI CCWGG 2 cut(s) 339, 495
BstNSI RCATGY 1 cut(s) 286
BstSCI CCNGG 2 cut(s) 337, 493
BstV1I GCAGC 1 cut(s) 30
BstXI CCANNNNNNTGG 1 cut(s) 206
BsuRI GGCC 1 cut(s) 63
BtgZI GCGATG 1 cut(s) 96
BtsCI GGATG 1 cut(s) 251
BtsIMutI CAGTG 1 cut(s) 186
Cac8I GCNNGC 1 cut(s) 453
CciI TCATGA 1 cut(s) 589
CfoI GCGC 2 cut(s) 388, 457
Cfr10I RCCGGY 2 cut(s) 409, 656
Cfr13I GGNCC 1 cut(s) 248
Csp6I GTAC 1 cut(s) 436
CviAII CATG 9 cut(s) 200, 229, 283, 287, 381, 403, 572, 578, 590
CviQI GTAC 1 cut(s) 436
DraIII CACNNNGTG 1 cut(s) 181
DrdI GACNNNNNNGTC 1 cut(s) 470
DseDI GACNNNNNNGTC 1 cut(s) 470
EaeI YGGCCR 1 cut(s) 61
Eam1104I CTCTTC 2 cut(s) 339, 354
EarI CTCTTC 2 cut(s) 339, 354
Eco32I GATATC 2 cut(s) 171, 243
Eco47I GGWCC 1 cut(s) 248
EcoRII CCWGG 2 cut(s) 337, 493
EcoRV GATATC 2 cut(s) 171, 243
EcoT22I ATGCAT 1 cut(s) 288
FaeI CATG 9 cut(s) 203, 232, 286, 290, 384, 406, 575, 581, 593
FatI CATG 9 cut(s) 199, 228, 282, 286, 380, 402, 571, 577, 589
FauI CCCGC 1 cut(s) 134
Fnu4HI GCNGC 3 cut(s) 19, 64, 315
FokI GGATG 1 cut(s) 238
Fsp4HI GCNGC 3 cut(s) 19, 64, 315
FspBI CTAG 1 cut(s) 676
GlaI GCGC 2 cut(s) 387, 456
GluI GCNGC 3 cut(s) 19, 64, 315
GsuI CTGGAG 2 cut(s) 113, 360
HaeIII GGCC 1 cut(s) 63
HapII CCGG 2 cut(s) 410, 657
HhaI GCGC 2 cut(s) 388, 457
Hin1II CATG 9 cut(s) 203, 232, 286, 290, 384, 406, 575, 581, 593
Hin6I GCGC 2 cut(s) 386, 455
HinP1I GCGC 2 cut(s) 386, 455
HindIII AAGCTT 1 cut(s) 297
HinfI GANTC 2 cut(s) 397, 483
HpaII CCGG 2 cut(s) 410, 657
HphI GGTGA 3 cut(s) 124, 656, 664
Hpy188I TCNGA 1 cut(s) 546
Hpy188III TCNNGA 4 cut(s) 28, 92, 236, 590
HpyAV CCTTC 4 cut(s) 182, 334, 473, 576
HpyCH4III ACNGT 3 cut(s) 181, 274, 652
HpyCH4V TGCA 2 cut(s) 278, 286
HpyF10VI GCNNNNNNNGC 7 cut(s) 18, 60, 305, 314, 320, 329, 527
Hsp92II CATG 9 cut(s) 203, 232, 286, 290, 384, 406, 575, 581, 593
HspAI GCGC 2 cut(s) 386, 455
LguI GCTCTTC 2 cut(s) 339, 354
LmnI GCTCC 3 cut(s) 526, 553, 607
Lsp1109I GCAGC 1 cut(s) 30
LweI GCATC 2 cut(s) 73, 310
MaeI CTAG 1 cut(s) 676
MaeIII GTNAC 1 cut(s) 652
MboII GAAGA 6 cut(s) 17, 133, 326, 371, 488, 559
MhlI GDGCHC 1 cut(s) 502
MluCI AATT 1 cut(s) 664
MmeI TCCRAC 3 cut(s) 238, 348, 548
MnlI CCTC 4 cut(s) 24, 185, 243, 335
Mph1103I ATGCAT 1 cut(s) 288
MroXI GAANNNNTTC 1 cut(s) 540
MseI TTAA 1 cut(s) 423
MslI CAYNNNNRTG 1 cut(s) 576
MspI CCGG 2 cut(s) 410, 657
MspR9I CCNGG 2 cut(s) 339, 495
MvaI CCWGG 2 cut(s) 339, 495
MwoI GCNNNNNNNGC 7 cut(s) 18, 60, 305, 314, 320, 329, 527
NlaIII CATG 9 cut(s) 203, 232, 286, 290, 384, 406, 575, 581, 593
NmeAIII GCCGAG 1 cut(s) 481
NsiI ATGCAT 1 cut(s) 288
NspI RCATGY 1 cut(s) 286
PagI TCATGA 1 cut(s) 589
PciSI GCTCTTC 2 cut(s) 339, 354
PdmI GAANNNNTTC 1 cut(s) 540
PfeI GAWTC 2 cut(s) 397, 483
PflMI CCANNNNNTGG 1 cut(s) 257
PfoI TCCNGGA 1 cut(s) 337
PkrI GCNGC 3 cut(s) 20, 65, 316
Psp6I CCWGG 2 cut(s) 337, 493
PspGI CCWGG 2 cut(s) 337, 493
PspPI GGNCC 1 cut(s) 248
RsaI GTAC 1 cut(s) 437
RsaNI GTAC 1 cut(s) 436
RseI CAYNNNNRTG 1 cut(s) 576
SapI GCTCTTC 2 cut(s) 339, 354
SaqAI TTAA 1 cut(s) 423
SatI GCNGC 3 cut(s) 19, 64, 315
Sau96I GGNCC 1 cut(s) 248
ScrFI CCNGG 2 cut(s) 339, 495
SduI GDGCHC 1 cut(s) 502
SetI ASST 9 cut(s) 196, 226, 301, 366, 449, 499, 599, 646, 677
SfaNI GCATC 2 cut(s) 73, 310
SinI GGWCC 1 cut(s) 248
SmiMI CAYNNNNRTG 1 cut(s) 576
SmlI CTYRAG 2 cut(s) 236, 309
SmoI CTYRAG 2 cut(s) 236, 309
Sse9I AATT 1 cut(s) 664
SsiI CCGC 3 cut(s) 64, 141, 314
SspMI CTAG 1 cut(s) 676
StyD4I CCNGG 2 cut(s) 337, 493
TaaI ACNGT 3 cut(s) 181, 274, 652
TaqI TCGA 1 cut(s) 474
TasI AATT 1 cut(s) 664
TauI GCSGC 2 cut(s) 66, 317
TfiI GAWTC 2 cut(s) 397, 483
Tru1I TTAA 1 cut(s) 423
Tru9I TTAA 1 cut(s) 423
TscAI CASTG 1 cut(s) 186
TseI GCWGC 1 cut(s) 18
TspDTI ATGAA 7 cut(s) 120, 201, 303, 316, 419, 475, 606
TspGWI ACGGA 1 cut(s) 244
TspRI CASTG 1 cut(s) 186
Van91I CCANNNNNTGG 1 cut(s) 257
VpaK11BI GGWCC 1 cut(s) 248
XceI RCATGY 1 cut(s) 286
XmnI GAANNNNTTC 1 cut(s) 540
XspI CTAG 1 cut(s) 676
Zsp2I ATGCAT 1 cut(s) 288
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.