pycom15g05320
NAC Family

Cytokinin-activating enzyme working in the direct activation pathway. Phosphoribohydrolase that converts inactive cytokinin nucleotides to the biologically active free-base forms

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0001203
Physical Location & Seq
Reverse (-)
3190372 .. 3192319
1948 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g05320.3

Sequence Viewer

Length: 678 bp
ATGGAAGAGAGCAAACGCAGCAAGTTCAGGAGGATTTGTGTGTTCTGTGGAAGCAACTCTGGCCGCAGGAAAGTGTTCAGTGACGCTACTCTGGAGTTGGGCAATGAACTGGTGAAAAGGAAGATAGATTTGGTGTATGGCGGGGGAAGTGTCGGGTTGATGGGTTTGATATCGCAGACGGTGTATGATGGAGGTTGCCATGTTCTTGGGGTCATTCCGAAAGCTCTCATGCCTCTTGAGATATCTGGTCCATCCGTTGGAGAAGTAAGAACAGTTACAGACATGCATGAACGCAAAGCTTTGATGGCTCAAGAGGCTGATGCCTTCATTGCTCTTCCTGGAGGATACGGAACTATGGAAGAGCTGTTGGAGATAATAACATGGGCGCAACTTGGGATTCATAGGAAGCCGGTTGGTTTGCTTAATGTAGATGGCTACTATAATGCCTTGCTTGCGCTATTTGACAATGGTGTCGAAGAAGGATTCATCAAGACAGGTGCTCGGCATATAGTTCTCTCTGCTCCAACAGCCAAAGAACTTGTTGAGAAGATGGAGCAATACACTCCTTCCCATGAACATATTGCTTCTCATGAAAGCTGGCAAATGGAACAACTTGGTAAACTCGGAGCCATGAAAGCGCAAGATGCAACTGTTATCAACGATAATTCTTTCACCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lysine_decarbox

Protein Analysis

226

Amino Acids

24.61

Weight (kDa)

5.82

Isoelectric Point (pI)

40.25

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 470
AccB7I CCANNNNNTGG 1 cut(s) 257
AciI CCGC 2 cut(s) 64, 141
AcoI YGGCCR 1 cut(s) 61
AfiI CCNNNNNNNGG 1 cut(s) 257
AjnI CCWGG 1 cut(s) 337
AjuI GAANNNNNNNTTGG 2 cut(s) 113, 145
AluBI AGCT 4 cut(s) 224, 299, 364, 597
AluI AGCT 4 cut(s) 224, 299, 364, 597
Alw21I GWGCWC 1 cut(s) 502
AoxI GGCC 1 cut(s) 61
ApeKI GCWGC 1 cut(s) 18
Asp700I GAANNNNTTC 1 cut(s) 74
AspLEI GCGC 3 cut(s) 388, 457, 640
AspS9I GGNCC 1 cut(s) 248
AsuHPI GGTGA 2 cut(s) 124, 664
AvaII GGWCC 1 cut(s) 248
Bbv12I GWGCWC 1 cut(s) 502
BbvI GCAGC 1 cut(s) 30
BccI CCATC 6 cut(s) 154, 182, 259, 298, 425, 544
BciT130I CCWGG 1 cut(s) 339
BciVI GTATCC 1 cut(s) 338
BfaI CTAG 1 cut(s) 676
BfuI GTATCC 1 cut(s) 338
BisI GCNGC 2 cut(s) 19, 64
BlsI GCNGC 2 cut(s) 20, 65
Bme1390I CCNGG 1 cut(s) 339
Bme18I GGWCC 1 cut(s) 248
BmgT120I GGNCC 1 cut(s) 248
BmiI GGNNCC 1 cut(s) 628
BmrFI CCNGG 1 cut(s) 339
BmsI GCATC 2 cut(s) 310, 634
BpmI CTGGAG 2 cut(s) 113, 360
BpuEI CTTGAG 2 cut(s) 257, 294
BsaXI ACNNNNNCTCC 2 cut(s) 22, 52
Bsc4I CCNNNNNNNGG 1 cut(s) 257
Bse118I RCCGGY 1 cut(s) 409
Bse1I ACTGG 1 cut(s) 114
Bse3DI GCAATG 2 cut(s) 109, 327
BseBI CCWGG 1 cut(s) 339
BseGI GGATG 1 cut(s) 251
BseLI CCNNNNNNNGG 1 cut(s) 257
BseMI GCAATG 2 cut(s) 109, 327
BseNI ACTGG 1 cut(s) 114
BseXI GCAGC 1 cut(s) 30
BshFI GGCC 1 cut(s) 63
BsiHKAI GWGCWC 1 cut(s) 502
BsiSI CCGG 1 cut(s) 410
BslI CCNNNNNNNGG 1 cut(s) 257
BsnI GGCC 1 cut(s) 63
Bsp1286I GDGCHC 1 cut(s) 502
BspACI CCGC 2 cut(s) 64, 141
BspANI GGCC 1 cut(s) 63
BspHI TCATGA 1 cut(s) 589
BspLI GGNNCC 1 cut(s) 628
BspQI GCTCTTC 2 cut(s) 339, 354
BsrDI GCAATG 2 cut(s) 109, 327
BsrFI RCCGGY 1 cut(s) 409
BsrI ACTGG 1 cut(s) 114
BssAI RCCGGY 1 cut(s) 409
Bst2UI CCWGG 1 cut(s) 339
Bst4CI ACNGT 3 cut(s) 181, 274, 652
Bst6I CTCTTC 2 cut(s) 339, 354
BstC8I GCNNGC 2 cut(s) 453, 599
BstF5I GGATG 1 cut(s) 251
BstHHI GCGC 3 cut(s) 388, 457, 640
BstMWI GCNNNNNNNGC 9 cut(s) 18, 60, 305, 314, 329, 452, 527, 635, 644
BstNI CCWGG 1 cut(s) 339
BstNSI RCATGY 1 cut(s) 286
BstSCI CCNGG 1 cut(s) 337
BstV1I GCAGC 1 cut(s) 30
BstXI CCANNNNNNTGG 1 cut(s) 206
BsuI GTATCC 1 cut(s) 338
BsuRI GGCC 1 cut(s) 63
BtsCI GGATG 1 cut(s) 251
BtsIMutI CAGTG 1 cut(s) 85
Cac8I GCNNGC 2 cut(s) 453, 599
CciI TCATGA 1 cut(s) 589
CfoI GCGC 3 cut(s) 388, 457, 640
Cfr10I RCCGGY 1 cut(s) 409
Cfr13I GGNCC 1 cut(s) 248
CseI GACGC 1 cut(s) 92
CviAII CATG 8 cut(s) 200, 229, 283, 287, 381, 572, 590, 631
DrdI GACNNNNNNGTC 1 cut(s) 470
DseDI GACNNNNNNGTC 1 cut(s) 470
EaeI YGGCCR 1 cut(s) 61
Eam1104I CTCTTC 2 cut(s) 339, 354
EarI CTCTTC 2 cut(s) 339, 354
Eco32I GATATC 2 cut(s) 171, 243
Eco47I GGWCC 1 cut(s) 248
EcoRII CCWGG 1 cut(s) 337
EcoRV GATATC 2 cut(s) 171, 243
EcoT22I ATGCAT 1 cut(s) 288
FaeI CATG 8 cut(s) 203, 232, 286, 290, 384, 575, 593, 634
FatI CATG 8 cut(s) 199, 228, 282, 286, 380, 571, 589, 630
FauI CCCGC 1 cut(s) 134
Fnu4HI GCNGC 2 cut(s) 19, 64
FokI GGATG 1 cut(s) 238
Fsp4HI GCNGC 2 cut(s) 19, 64
FspBI CTAG 1 cut(s) 676
GlaI GCGC 3 cut(s) 387, 456, 639
GluI GCNGC 2 cut(s) 19, 64
GsuI CTGGAG 2 cut(s) 113, 360
HaeIII GGCC 1 cut(s) 63
HapII CCGG 1 cut(s) 410
HgaI GACGC 1 cut(s) 92
HhaI GCGC 3 cut(s) 388, 457, 640
Hin1II CATG 8 cut(s) 203, 232, 286, 290, 384, 575, 593, 634
Hin6I GCGC 3 cut(s) 386, 455, 638
HinP1I GCGC 3 cut(s) 386, 455, 638
HindIII AAGCTT 1 cut(s) 297
HinfI GANTC 2 cut(s) 397, 483
HpaII CCGG 1 cut(s) 410
HphI GGTGA 2 cut(s) 124, 664
Hpy166II GTNNAC 1 cut(s) 620
Hpy188I TCNGA 2 cut(s) 219, 626
Hpy188III TCNNGA 6 cut(s) 28, 92, 236, 311, 490, 590
Hpy8I GTNNAC 1 cut(s) 620
HpyAV CCTTC 3 cut(s) 334, 473, 576
HpyCH4III ACNGT 3 cut(s) 181, 274, 652
HpyCH4V TGCA 2 cut(s) 286, 647
HpyF10VI GCNNNNNNNGC 9 cut(s) 18, 60, 305, 314, 329, 452, 527, 635, 644
Hsp92II CATG 8 cut(s) 203, 232, 286, 290, 384, 575, 593, 634
HspAI GCGC 3 cut(s) 386, 455, 638
LguI GCTCTTC 2 cut(s) 339, 354
LmnI GCTCC 3 cut(s) 526, 553, 626
Lsp1109I GCAGC 1 cut(s) 30
LweI GCATC 2 cut(s) 310, 634
MaeI CTAG 1 cut(s) 676
MaeIII GTNAC 2 cut(s) 80, 274
MboII GAAGA 6 cut(s) 17, 133, 326, 371, 488, 559
MhlI GDGCHC 1 cut(s) 502
MluCI AATT 1 cut(s) 664
MmeI TCCRAC 3 cut(s) 238, 348, 548
MnlI CCTC 5 cut(s) 24, 185, 243, 307, 335
Mph1103I ATGCAT 1 cut(s) 288
MroXI GAANNNNTTC 1 cut(s) 74
MseI TTAA 1 cut(s) 423
MspI CCGG 1 cut(s) 410
MspR9I CCNGG 1 cut(s) 339
MvaI CCWGG 1 cut(s) 339
MwoI GCNNNNNNNGC 9 cut(s) 18, 60, 305, 314, 329, 452, 527, 635, 644
NlaIII CATG 8 cut(s) 203, 232, 286, 290, 384, 575, 593, 634
NlaIV GGNNCC 1 cut(s) 628
NmeAIII GCCGAG 1 cut(s) 481
NmuCI GTSAC 1 cut(s) 80
NsiI ATGCAT 1 cut(s) 288
NspI RCATGY 1 cut(s) 286
PagI TCATGA 1 cut(s) 589
PciSI GCTCTTC 2 cut(s) 339, 354
PdmI GAANNNNTTC 1 cut(s) 74
PfeI GAWTC 2 cut(s) 397, 483
PflMI CCANNNNNTGG 1 cut(s) 257
PfoI TCCNGGA 1 cut(s) 337
PkrI GCNGC 2 cut(s) 20, 65
Psp6I CCWGG 1 cut(s) 337
PspGI CCWGG 1 cut(s) 337
PspN4I GGNNCC 1 cut(s) 628
PspPI GGNCC 1 cut(s) 248
SapI GCTCTTC 2 cut(s) 339, 354
SaqAI TTAA 1 cut(s) 423
SatI GCNGC 2 cut(s) 19, 64
Sau96I GGNCC 1 cut(s) 248
ScrFI CCNGG 1 cut(s) 339
SduI GDGCHC 1 cut(s) 502
SetI ASST 7 cut(s) 196, 226, 301, 366, 499, 599, 677
SfaNI GCATC 2 cut(s) 310, 634
SinI GGWCC 1 cut(s) 248
SmlI CTYRAG 2 cut(s) 236, 309
SmoI CTYRAG 2 cut(s) 236, 309
Sse9I AATT 1 cut(s) 664
SsiI CCGC 2 cut(s) 64, 141
SspMI CTAG 1 cut(s) 676
StyD4I CCNGG 1 cut(s) 337
TaaI ACNGT 3 cut(s) 181, 274, 652
TaqI TCGA 1 cut(s) 474
TasI AATT 1 cut(s) 664
TauI GCSGC 1 cut(s) 66
TfiI GAWTC 2 cut(s) 397, 483
Tru1I TTAA 1 cut(s) 423
Tru9I TTAA 1 cut(s) 423
TscAI CASTG 1 cut(s) 85
TseFI GTSAC 1 cut(s) 80
TseI GCWGC 1 cut(s) 18
Tsp45I GTSAC 1 cut(s) 80
TspDTI ATGAA 8 cut(s) 120, 303, 316, 389, 475, 588, 606, 647
TspGWI ACGGA 2 cut(s) 244, 363
TspRI CASTG 1 cut(s) 85
Van91I CCANNNNNTGG 1 cut(s) 257
VpaK11BI GGWCC 1 cut(s) 248
XceI RCATGY 1 cut(s) 286
XmnI GAANNNNTTC 1 cut(s) 74
XspI CTAG 1 cut(s) 676
Zsp2I ATGCAT 1 cut(s) 288
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.