Prupe.1G409400_v2.0.a1
NAC Family

Cytokinin-activating enzyme working in the direct activation pathway. Phosphoribohydrolase that converts inactive cytokinin nucleotides to the biologically active free-base forms

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0001203
Physical Location & Seq
Reverse (-)
35821398 .. 35824359
2962 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G409400.7

Sequence Viewer

Length: 708 bp
ATGGAAGAGAGCAACGCAAGGAGCAAGTTCAAGAGGATTTGTGTGTTCTGTGGAAGCAACTCTGGCCGCAGAAAAGTATTCAGTGATGCTGCTCTTGAGTTGGGCAATGAACTGGTCAAGAGGAAGATAGATTTGGTGTATGGTGGGGGAAGTGTCGGGTTGATGGGTTTAATATCCCAGACAGTGTACGATGGAGGTTGCCATGTTCTTGGGGTCATTCCAAAAGCTCTCATGCCTCTTGAGATATCTGGTCCAACTGTTGGAGAAGTAAGAACAGTTACAGACATGCATGAGCGTAAGGCTTCTATGGCTCAAGAAGCTGATGCCTTTATTGCTCTTCCTGGAGGATATGGAACTATGGAAGAGCTGTTGGAGATAATCACATGGGCCCAACTTGGGATTCATATGAAGCCGGTTGGTTTGCTTAATGTTGATGGGTACTATAATTCCTTGCTGGCACTATTTGACAATGGTGTTGAAGAAGGTTTCATCAAGCCATGTGCTCGGCATATAGTTGTCTCTGCTCCAACAGCCAAAGAACTTGTGGTGAAGATGGAGCAATACACTCCTTCCCATGAACATGTTGCTTCTCATGAAAGCTGGCAAATGGAGCAACTTGATAACTCTCAAGCCAAGAAAACGCAAGATCGTTTCATGGCATACTTTGGTTTGAGGTCGATCGAAACGCTATTCCATGGTCGCATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lysine_decarbox

Protein Analysis

236

Amino Acids

25.92

Weight (kDa)

6.07

Isoelectric Point (pI)

39.06

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 260
AciI CCGC 1 cut(s) 67
AcoI YGGCCR 1 cut(s) 64
AfaI GTAC 2 cut(s) 188, 440
AfiI CCNNNNNNNGG 2 cut(s) 260, 396
AflIII ACRYGT 1 cut(s) 580
AgsI TTSAA 2 cut(s) 31, 479
AjnI CCWGG 1 cut(s) 340
AjuI GAANNNNNNNTTGG 4 cut(s) 116, 148, 384, 416
AluBI AGCT 4 cut(s) 227, 320, 367, 600
AluI AGCT 4 cut(s) 227, 320, 367, 600
Alw21I GWGCWC 1 cut(s) 505
Alw26I GTCTC 1 cut(s) 523
AoxI GGCC 2 cut(s) 64, 387
ApaI GGGCCC 1 cut(s) 391
ApeKI GCWGC 1 cut(s) 89
AspS9I GGNCC 3 cut(s) 251, 387, 388
AsuHPI GGTGA 1 cut(s) 559
AvaII GGWCC 1 cut(s) 251
BaeGI GKGCMC 1 cut(s) 391
BanII GRGCYC 1 cut(s) 391
Bbv12I GWGCWC 1 cut(s) 505
BbvI GCAGC 1 cut(s) 76
BccI CCATC 4 cut(s) 157, 185, 428, 547
BciT130I CCWGG 1 cut(s) 342
BcoDI GTCTC 1 cut(s) 523
BisI GCNGC 2 cut(s) 67, 90
BlsI GCNGC 2 cut(s) 68, 91
Bme1390I CCNGG 1 cut(s) 342
Bme18I GGWCC 1 cut(s) 251
BmgT120I GGNCC 3 cut(s) 251, 387, 388
BmiI GGNNCC 1 cut(s) 389
BmrFI CCNGG 1 cut(s) 342
BmsI GCATC 2 cut(s) 76, 313
BpmI CTGGAG 1 cut(s) 363
BpuEI CTTGAG 4 cut(s) 116, 260, 297, 612
BsaJI CCNNGG 1 cut(s) 694
Bsc4I CCNNNNNNNGG 2 cut(s) 260, 396
Bse118I RCCGGY 1 cut(s) 412
Bse1I ACTGG 1 cut(s) 117
Bse3DI GCAATG 1 cut(s) 112
BseBI CCWGG 1 cut(s) 342
BseDI CCNNGG 1 cut(s) 694
BseLI CCNNNNNNNGG 2 cut(s) 260, 396
BseMI GCAATG 1 cut(s) 112
BseNI ACTGG 1 cut(s) 117
BseSI GKGCMC 1 cut(s) 391
BseXI GCAGC 1 cut(s) 76
Bsh1285I CGRYCG 1 cut(s) 681
BshFI GGCC 2 cut(s) 66, 389
BsiEI CGRYCG 1 cut(s) 681
BsiHKAI GWGCWC 1 cut(s) 505
BsiSI CCGG 1 cut(s) 413
BslI CCNNNNNNNGG 2 cut(s) 260, 396
BsmAI GTCTC 1 cut(s) 523
BsnI GGCC 2 cut(s) 66, 389
Bsp120I GGGCCC 1 cut(s) 387
Bsp1286I GDGCHC 2 cut(s) 391, 505
Bsp143I GATC 2 cut(s) 646, 678
Bsp19I CCATGG 1 cut(s) 694
BspACI CCGC 1 cut(s) 67
BspANI GGCC 2 cut(s) 66, 389
BspHI TCATGA 1 cut(s) 592
BspLI GGNNCC 1 cut(s) 389
BspQI GCTCTTC 2 cut(s) 342, 357
BsrDI GCAATG 1 cut(s) 112
BsrFI RCCGGY 1 cut(s) 412
BsrI ACTGG 1 cut(s) 117
BssAI RCCGGY 1 cut(s) 412
BssECI CCNNGG 1 cut(s) 694
BssMI GATC 2 cut(s) 646, 678
BssT1I CCWWGG 1 cut(s) 694
Bst2UI CCWGG 1 cut(s) 342
Bst4CI ACNGT 3 cut(s) 184, 259, 277
Bst6I CTCTTC 2 cut(s) 342, 357
BstC8I GCNNGC 2 cut(s) 456, 602
BstDSI CCRYGG 1 cut(s) 694
BstKTI GATC 2 cut(s) 649, 681
BstMAI GTCTC 1 cut(s) 523
BstMBI GATC 2 cut(s) 646, 678
BstMCI CGRYCG 1 cut(s) 681
BstMWI GCNNNNNNNGC 6 cut(s) 63, 308, 317, 332, 530, 610
BstNI CCWGG 1 cut(s) 342
BstNSI RCATGY 2 cut(s) 289, 584
BstSCI CCNGG 1 cut(s) 340
BstSLI GKGCMC 1 cut(s) 391
BstV1I GCAGC 1 cut(s) 76
BstXI CCANNNNNNTGG 1 cut(s) 209
BsuRI GGCC 2 cut(s) 66, 389
BtgI CCRYGG 1 cut(s) 694
BtsIMutI CAGTG 2 cut(s) 88, 189
Cac8I GCNNGC 2 cut(s) 456, 602
CciI TCATGA 1 cut(s) 592
Cfr10I RCCGGY 1 cut(s) 412
Cfr13I GGNCC 3 cut(s) 251, 387, 388
Csp6I GTAC 2 cut(s) 187, 439
CviQI GTAC 2 cut(s) 187, 439
DpnI GATC 2 cut(s) 648, 680
DpnII GATC 2 cut(s) 646, 678
EaeI YGGCCR 1 cut(s) 64
Eam1104I CTCTTC 2 cut(s) 342, 357
EarI CTCTTC 2 cut(s) 342, 357
Eco130I CCWWGG 1 cut(s) 694
Eco24I GRGCYC 1 cut(s) 391
Eco32I GATATC 1 cut(s) 246
Eco47I GGWCC 1 cut(s) 251
EcoRII CCWGG 1 cut(s) 340
EcoRV GATATC 1 cut(s) 246
EcoT14I CCWWGG 1 cut(s) 694
EcoT22I ATGCAT 1 cut(s) 291
EcoT38I GRGCYC 1 cut(s) 391
ErhI CCWWGG 1 cut(s) 694
FauNDI CATATG 1 cut(s) 405
Fnu4HI GCNGC 2 cut(s) 67, 90
FriOI GRGCYC 1 cut(s) 391
Fsp4HI GCNGC 2 cut(s) 67, 90
GluI GCNGC 2 cut(s) 67, 90
GsuI CTGGAG 1 cut(s) 363
HaeIII GGCC 2 cut(s) 66, 389
HapII CCGG 1 cut(s) 413
HinfI GANTC 1 cut(s) 400
HpaII CCGG 1 cut(s) 413
HphI GGTGA 1 cut(s) 559
Hpy166II GTNNAC 1 cut(s) 187
Hpy188III TCNNGA 6 cut(s) 31, 95, 118, 239, 314, 593
Hpy8I GTNNAC 1 cut(s) 187
HpyAV CCTTC 2 cut(s) 476, 579
HpyCH4III ACNGT 3 cut(s) 184, 259, 277
HpyCH4V TGCA 1 cut(s) 289
HpyF10VI GCNNNNNNNGC 6 cut(s) 63, 308, 317, 332, 530, 610
Kzo9I GATC 2 cut(s) 646, 678
LguI GCTCTTC 2 cut(s) 342, 357
LmnI GCTCC 4 cut(s) 21, 529, 556, 610
LpnPI CCDG 9 cut(s) 48, 98, 191, 234, 327, 354, 426, 440, 586
Lsp1109I GCAGC 1 cut(s) 76
LweI GCATC 2 cut(s) 76, 313
MaeIII GTNAC 1 cut(s) 277
MalI GATC 2 cut(s) 648, 680
MboI GATC 2 cut(s) 646, 678
MboII GAAGA 6 cut(s) 17, 136, 329, 374, 491, 562
MhlI GDGCHC 2 cut(s) 391, 505
MluCI AATT 1 cut(s) 445
MmeI TCCRAC 4 cut(s) 241, 278, 351, 551
MnlI CCTC 6 cut(s) 27, 114, 188, 246, 338, 666
Mph1103I ATGCAT 1 cut(s) 291
MseI TTAA 2 cut(s) 170, 426
MslI CAYNNNNRTG 1 cut(s) 579
MspI CCGG 1 cut(s) 413
MspR9I CCNGG 1 cut(s) 342
MvaI CCWGG 1 cut(s) 342
MwoI GCNNNNNNNGC 6 cut(s) 63, 308, 317, 332, 530, 610
NcoI CCATGG 1 cut(s) 694
NdeI CATATG 1 cut(s) 405
NdeII GATC 2 cut(s) 646, 678
NlaIV GGNNCC 1 cut(s) 389
NmeAIII GCCGAG 1 cut(s) 484
NsiI ATGCAT 1 cut(s) 291
NspI RCATGY 2 cut(s) 289, 584
PagI TCATGA 1 cut(s) 592
PciI ACATGT 1 cut(s) 580
PciSI GCTCTTC 2 cut(s) 342, 357
PfeI GAWTC 1 cut(s) 400
PflMI CCANNNNNTGG 1 cut(s) 260
PfoI TCCNGGA 1 cut(s) 340
PkrI GCNGC 2 cut(s) 68, 91
Ple19I CGATCG 1 cut(s) 681
PscI ACATGT 1 cut(s) 580
Psp6I CCWGG 1 cut(s) 340
PspGI CCWGG 1 cut(s) 340
PspN4I GGNNCC 1 cut(s) 389
PspOMI GGGCCC 1 cut(s) 387
PspPI GGNCC 3 cut(s) 251, 387, 388
PvuI CGATCG 1 cut(s) 681
RsaI GTAC 2 cut(s) 188, 440
RsaNI GTAC 2 cut(s) 187, 439
RseI CAYNNNNRTG 1 cut(s) 579
SapI GCTCTTC 2 cut(s) 342, 357
SaqAI TTAA 2 cut(s) 170, 426
SatI GCNGC 2 cut(s) 67, 90
Sau3AI GATC 2 cut(s) 646, 678
Sau96I GGNCC 3 cut(s) 251, 387, 388
ScrFI CCNGG 1 cut(s) 342
SduI GDGCHC 2 cut(s) 391, 505
SetI ASST 7 cut(s) 199, 229, 322, 369, 487, 602, 677
SfaNI GCATC 2 cut(s) 76, 313
SinI GGWCC 1 cut(s) 251
SmiMI CAYNNNNRTG 1 cut(s) 579
SmlI CTYRAG 4 cut(s) 95, 239, 312, 627
SmoI CTYRAG 4 cut(s) 95, 239, 312, 627
Sse9I AATT 1 cut(s) 445
SsiI CCGC 1 cut(s) 67
StyD4I CCNGG 1 cut(s) 340
StyI CCWWGG 1 cut(s) 694
TaaI ACNGT 3 cut(s) 184, 259, 277
TaqI TCGA 2 cut(s) 677, 681
TasI AATT 1 cut(s) 445
TauI GCSGC 1 cut(s) 69
TfiI GAWTC 1 cut(s) 400
Tru1I TTAA 2 cut(s) 170, 426
Tru9I TTAA 2 cut(s) 170, 426
TscAI CASTG 2 cut(s) 88, 189
TseI GCWGC 1 cut(s) 89
TspDTI ATGAA 7 cut(s) 123, 392, 422, 478, 591, 609, 643
TspRI CASTG 2 cut(s) 88, 189
Van91I CCANNNNNTGG 1 cut(s) 260
VpaK11BI GGWCC 1 cut(s) 251
XceI RCATGY 2 cut(s) 289, 584
XcmI CCANNNNNNNNNTGG 1 cut(s) 541
Zsp2I ATGCAT 1 cut(s) 291
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.