pycom15g01200
NAC Family

Cytokinin-activating enzyme working in the direct activation pathway. Phosphoribohydrolase that converts inactive cytokinin nucleotides to the biologically active free-base forms

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0001203
Physical Location & Seq
Reverse (-)
638604 .. 641366
2763 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g01200.4

Sequence Viewer

Length: 861 bp
ATGGTGATGAGAACCAATACCATTAATTTCATTCCCTCAACTTTTGTCAATGGCTTTCATAGCGATTGCTTGACTGTTGAACACAACAAACACCTTCACGTTAACCTCTTTTCTCTCTCCAGTTCGTTCATTCTCTCTCTCTCTCTCTCTATCTCTCTCGACATTTCTCAATCGAAGCAAAAAACCCAGTTGGATTCAGGGCTTTCTGCAATGGAAAATCAGCAGCAGATTGCAATGAAATCGAGATTCAGCCGTATCTGCGTTTTCTGTGGCAGCAGTGCTGGCAAGAACCCGAGCTACCAGCTCGCTGCCATTAAACTCGGCAAAGAATTGGTTGAAAGAAGCATCGACTTGGTTTATGGAGGAGGAAGCATTGGACTGATGGGCCAGGTCTCCCAAGTTGTCTATGATGGTGGCCGCCACGTGTTGGGAGTAATTCCCACAACTCTCATGCCAAGAGAGATCACAGGAGAGTCAGTGGGAGAAGTAAGAGCTGTATCAGGCATGCACCAACGCAAAGCAGAAATGGCTCGCCAAGCTGATGCCTTTATTGCCTTGCCAGGTGGCTATGGTACCTTGGAAGAACTGTTGGAAGTGATCACTTGGGCACAGTTGGGAATCCATGACAAACCGGTGGGGTTGTTAAATGTGGATGGATACTACAACTCACTGCTATCCTTCATAGACAAGGCTGTGGATGAAGGTTTCATTGACCCTGCAGCTCGTCACATTATTGTCTCAGCCGAAACACCCCACGAACTCATGTGCAAACTAGAGGAATATGCTCCAAAGCATTGTGGACTGAGTTGGGAGATGGAGCAACAACTGGAATACACGGCAAAGTCAGACATTGCACGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lysine_decarbox

Protein Analysis

287

Amino Acids

31.38

Weight (kDa)

5.8

Isoelectric Point (pI)

31.47

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 572
AccB1I GGYRCC 1 cut(s) 572
AccB7I CCANNNNNTGG 1 cut(s) 427
AciI CCGC 1 cut(s) 418
AcoI YGGCCR 1 cut(s) 415
AcvI CACGTG 1 cut(s) 424
AfaI GTAC 1 cut(s) 574
AfiI CCNNNNNNNGG 1 cut(s) 427
AflIII ACRYGT 1 cut(s) 423
AgeI ACCGGT 1 cut(s) 631
AgsI TTSAA 2 cut(s) 80, 338
AjnI CCWGG 2 cut(s) 387, 559
AluBI AGCT 5 cut(s) 297, 304, 494, 539, 722
AluI AGCT 5 cut(s) 297, 304, 494, 539, 722
Alw26I GTCTC 2 cut(s) 397, 742
Ama87I CYCGRG 1 cut(s) 292
AoxI GGCC 2 cut(s) 385, 415
ApeKI GCWGC 4 cut(s) 223, 273, 308, 719
AseI ATTAAT 1 cut(s) 24
AsiGI ACCGGT 1 cut(s) 631
Asp718I GGTACC 1 cut(s) 572
AspS9I GGNCC 1 cut(s) 385
AsuHPI GGTGA 1 cut(s) 16
AvaI CYCGRG 1 cut(s) 292
BaeGI GKGCMC 1 cut(s) 610
BanI GGYRCC 1 cut(s) 572
BbrPI CACGTG 1 cut(s) 424
BbvI GCAGC 4 cut(s) 235, 285, 295, 731
BccI CCATC 4 cut(s) 376, 404, 647, 808
BceAI ACGGC 2 cut(s) 237, 852
BcgI CGANNNNNNTGC 2 cut(s) 222, 256
BciT130I CCWGG 2 cut(s) 389, 561
BciVI GTATCC 1 cut(s) 650
BclI TGATCA 1 cut(s) 597
BcoDI GTCTC 2 cut(s) 397, 742
BfaI CTAG 1 cut(s) 773
BfmI CTRYAG 1 cut(s) 717
BfuI GTATCC 1 cut(s) 650
BisI GCNGC 5 cut(s) 224, 274, 309, 418, 720
BlsI GCNGC 5 cut(s) 225, 275, 310, 419, 721
Bme1390I CCNGG 2 cut(s) 389, 561
BmeT110I CYCGRG 1 cut(s) 292
BmgT120I GGNCC 1 cut(s) 385
BmiI GGNNCC 1 cut(s) 574
BmrFI CCNGG 2 cut(s) 389, 561
BmrI ACTGGG 1 cut(s) 181
BmsI GCATC 2 cut(s) 354, 532
BmuI ACTGGG 1 cut(s) 181
BpmI CTGGAG 1 cut(s) 103
BsaAI YACGTR 1 cut(s) 424
BsaI GGTCTC 1 cut(s) 397
BsaJI CCNNGG 1 cut(s) 576
BsaWI WCCGGW 1 cut(s) 631
BsaXI ACNNNNNCTCC 2 cut(s) 462, 492
Bsc4I CCNNNNNNNGG 1 cut(s) 427
Bse118I RCCGGY 1 cut(s) 631
Bse1I ACTGG 3 cut(s) 120, 187, 831
Bse3DI GCAATG 3 cut(s) 216, 240, 849
BseBI CCWGG 2 cut(s) 389, 561
BseDI CCNNGG 1 cut(s) 576
BseGI GGATG 2 cut(s) 658, 703
BseLI CCNNNNNNNGG 1 cut(s) 427
BseMI GCAATG 3 cut(s) 216, 240, 849
BseMII CTCAG 2 cut(s) 753, 794
BseNI ACTGG 3 cut(s) 120, 187, 831
BseRI GAGGAG 1 cut(s) 378
BseSI GKGCMC 1 cut(s) 610
BseXI GCAGC 4 cut(s) 235, 285, 295, 731
BshFI GGCC 2 cut(s) 387, 417
BshNI GGYRCC 1 cut(s) 572
BshTI ACCGGT 1 cut(s) 631
BsiHKCI CYCGRG 1 cut(s) 292
BsiSI CCGG 1 cut(s) 632
BslI CCNNNNNNNGG 1 cut(s) 427
BsmAI GTCTC 2 cut(s) 397, 742
BsnI GGCC 2 cut(s) 387, 417
Bso31I GGTCTC 1 cut(s) 397
BsoBI CYCGRG 1 cut(s) 292
Bsp1286I GDGCHC 1 cut(s) 610
Bsp143I GATC 2 cut(s) 462, 597
BspACI CCGC 1 cut(s) 418
BspANI GGCC 2 cut(s) 387, 417
BspCNI CTCAG 2 cut(s) 752, 795
BspLI GGNNCC 1 cut(s) 574
BspMAI CTGCAG 1 cut(s) 721
BspT107I GGYRCC 1 cut(s) 572
BspTNI GGTCTC 1 cut(s) 397
BsrDI GCAATG 3 cut(s) 216, 240, 849
BsrFI RCCGGY 1 cut(s) 631
BsrI ACTGG 3 cut(s) 120, 187, 831
BssAI RCCGGY 1 cut(s) 631
BssECI CCNNGG 1 cut(s) 576
BssMI GATC 2 cut(s) 462, 597
BssT1I CCWWGG 1 cut(s) 576
Bst2UI CCWGG 2 cut(s) 389, 561
Bst4CI ACNGT 3 cut(s) 76, 588, 612
BstBAI YACGTR 1 cut(s) 424
BstC8I GCNNGC 4 cut(s) 283, 306, 506, 532
BstDEI CTNAG 2 cut(s) 739, 803
BstF5I GGATG 2 cut(s) 658, 703
BstKTI GATC 2 cut(s) 465, 600
BstMAI GTCTC 2 cut(s) 397, 742
BstMBI GATC 2 cut(s) 462, 597
BstMWI GCNNNNNNNGC 6 cut(s) 60, 258, 282, 527, 536, 551
BstNI CCWGG 2 cut(s) 389, 561
BstNSI RCATGY 1 cut(s) 508
BstSCI CCNGG 2 cut(s) 387, 559
BstSFI CTRYAG 1 cut(s) 717
BstSLI GKGCMC 1 cut(s) 610
BstV1I GCAGC 4 cut(s) 235, 285, 295, 731
BsuI GTATCC 1 cut(s) 650
BsuRI GGCC 2 cut(s) 387, 417
BtsCI GGATG 2 cut(s) 658, 703
BtsI GCAGTG 2 cut(s) 283, 668
BtsIMutI CAGTG 3 cut(s) 283, 483, 668
Cac8I GCNNGC 4 cut(s) 283, 306, 506, 532
Cfr10I RCCGGY 1 cut(s) 631
Cfr13I GGNCC 1 cut(s) 385
Csp6I GTAC 1 cut(s) 573
CspAI ACCGGT 1 cut(s) 631
CviAII CATG 4 cut(s) 451, 505, 623, 763
CviQI GTAC 1 cut(s) 573
DdeI CTNAG 2 cut(s) 739, 803
DpnI GATC 2 cut(s) 464, 599
DpnII GATC 2 cut(s) 462, 597
EaeI YGGCCR 1 cut(s) 415
Eco130I CCWWGG 1 cut(s) 576
Eco31I GGTCTC 1 cut(s) 397
Eco72I CACGTG 1 cut(s) 424
Eco88I CYCGRG 1 cut(s) 292
EcoRII CCWGG 2 cut(s) 387, 559
EcoT14I CCWWGG 1 cut(s) 576
ErhI CCWWGG 1 cut(s) 576
FaeI CATG 4 cut(s) 454, 508, 626, 766
FatI CATG 4 cut(s) 450, 504, 622, 762
FbaI TGATCA 1 cut(s) 597
Fnu4HI GCNGC 5 cut(s) 224, 274, 309, 418, 720
FokI GGATG 2 cut(s) 665, 710
Fsp4HI GCNGC 5 cut(s) 224, 274, 309, 418, 720
FspBI CTAG 1 cut(s) 773
GluI GCNGC 5 cut(s) 224, 274, 309, 418, 720
GsuI CTGGAG 1 cut(s) 103
HaeIII GGCC 2 cut(s) 387, 417
HapII CCGG 1 cut(s) 632
Hin1II CATG 4 cut(s) 454, 508, 626, 766
HincII GTYRAC 1 cut(s) 103
HindII GTYRAC 1 cut(s) 103
HinfI GANTC 4 cut(s) 194, 246, 473, 618
HpaI GTTAAC 1 cut(s) 103
HpaII CCGG 1 cut(s) 632
HphI GGTGA 1 cut(s) 16
Hpy166II GTNNAC 2 cut(s) 103, 800
Hpy188I TCNGA 1 cut(s) 847
Hpy188III TCNNGA 2 cut(s) 158, 243
Hpy8I GTNNAC 2 cut(s) 103, 800
HpyAV CCTTC 3 cut(s) 104, 688, 695
HpyCH4III ACNGT 3 cut(s) 76, 588, 612
HpyCH4IV ACGT 3 cut(s) 99, 423, 856
HpyCH4V TGCA 6 cut(s) 209, 233, 508, 719, 768, 854
HpyF10VI GCNNNNNNNGC 6 cut(s) 60, 258, 282, 527, 536, 551
HpyF3I CTNAG 2 cut(s) 739, 803
HpySE526I ACGT 3 cut(s) 99, 423, 856
Hsp92II CATG 4 cut(s) 454, 508, 626, 766
KpnI GGTACC 1 cut(s) 576
Ksp22I TGATCA 1 cut(s) 597
KspAI GTTAAC 1 cut(s) 103
Kzo9I GATC 2 cut(s) 462, 597
LmnI GCTCC 2 cut(s) 790, 817
Lsp1109I GCAGC 4 cut(s) 235, 285, 295, 731
LweI GCATC 2 cut(s) 354, 532
MaeI CTAG 1 cut(s) 773
MaeII ACGT 3 cut(s) 99, 423, 856
MaeIII GTNAC 1 cut(s) 725
MalI GATC 2 cut(s) 464, 599
MboI GATC 2 cut(s) 462, 597
MboII GAAGA 1 cut(s) 593
MhlI GDGCHC 1 cut(s) 610
MluCI AATT 3 cut(s) 25, 329, 435
MlyI GAGTC 1 cut(s) 482
MmeI TCCRAC 2 cut(s) 171, 570
MnlI CCTC 5 cut(s) 46, 116, 356, 359, 769
MseI TTAA 4 cut(s) 24, 102, 315, 644
MspI CCGG 1 cut(s) 632
MspR9I CCNGG 2 cut(s) 389, 561
MvaI CCWGG 2 cut(s) 389, 561
MwoI GCNNNNNNNGC 6 cut(s) 60, 258, 282, 527, 536, 551
NdeII GATC 2 cut(s) 462, 597
NlaIII CATG 4 cut(s) 454, 508, 626, 766
NlaIV GGNNCC 1 cut(s) 574
NmeAIII GCCGAG 1 cut(s) 300
NmuCI GTSAC 1 cut(s) 725
NspI RCATGY 1 cut(s) 508
PaeI GCATGC 1 cut(s) 508
PfeI GAWTC 3 cut(s) 194, 246, 618
PflMI CCANNNNNTGG 1 cut(s) 427
PinAI ACCGGT 1 cut(s) 631
PkrI GCNGC 5 cut(s) 225, 275, 310, 419, 721
PleI GAGTC 1 cut(s) 481
PmaCI CACGTG 1 cut(s) 424
PmlI CACGTG 1 cut(s) 424
PpsI GAGTC 1 cut(s) 481
Ppu21I YACGTR 1 cut(s) 424
PshBI ATTAAT 1 cut(s) 24
Psp6I CCWGG 2 cut(s) 387, 559
PspCI CACGTG 1 cut(s) 424
PspGI CCWGG 2 cut(s) 387, 559
PspN4I GGNNCC 1 cut(s) 574
PspPI GGNCC 1 cut(s) 385
PsrI GAACNNNNNNTAC 2 cut(s) 281, 313
PstI CTGCAG 1 cut(s) 721
RsaI GTAC 1 cut(s) 574
RsaNI GTAC 1 cut(s) 573
SaqAI TTAA 4 cut(s) 24, 102, 315, 644
SatI GCNGC 5 cut(s) 224, 274, 309, 418, 720
Sau3AI GATC 2 cut(s) 462, 597
Sau96I GGNCC 1 cut(s) 385
SchI GAGTC 1 cut(s) 482
ScrFI CCNGG 2 cut(s) 389, 561
SduI GDGCHC 1 cut(s) 610
SfaNI GCATC 2 cut(s) 354, 532
SfcI CTRYAG 1 cut(s) 717
SphI GCATGC 1 cut(s) 508
Sse9I AATT 3 cut(s) 25, 329, 435
SsiI CCGC 1 cut(s) 418
SspMI CTAG 1 cut(s) 773
StyD4I CCNGG 2 cut(s) 387, 559
StyI CCWWGG 1 cut(s) 576
TaaI ACNGT 3 cut(s) 76, 588, 612
TaiI ACGT 3 cut(s) 102, 426, 859
TaqI TCGA 4 cut(s) 159, 173, 242, 348
TasI AATT 3 cut(s) 25, 329, 435
TauI GCSGC 1 cut(s) 420
TfiI GAWTC 3 cut(s) 194, 246, 618
Tru1I TTAA 4 cut(s) 24, 102, 315, 644
Tru9I TTAA 4 cut(s) 24, 102, 315, 644
TscAI CASTG 3 cut(s) 283, 483, 675
TseFI GTSAC 1 cut(s) 725
TseI GCWGC 4 cut(s) 223, 273, 308, 719
Tsp45I GTSAC 1 cut(s) 725
TspDTI ATGAA 7 cut(s) 19, 47, 118, 251, 670, 697, 714
TspRI CASTG 3 cut(s) 283, 483, 675
Van91I CCANNNNNTGG 1 cut(s) 427
VspI ATTAAT 1 cut(s) 24
XceI RCATGY 1 cut(s) 508
XspI CTAG 1 cut(s) 773
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.