Rh6BG473600

Cytokinin riboside 5'-monophosphate phosphoribohydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0001203
Physical Location & Seq
Reverse (-)
68018062 .. 68019403
1342 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG473600.1

Sequence Viewer

Length: 462 bp
ATGGAGATGATTCAAGAACAACAACAACAACAAAAGGGTGTAATGAAATCTTCAAGATTCAAGCGTATCTGTGTTTTCTGTGGTAGCAGTGCTGGAAAGAACCCAAGTTATCAGCTTGCTGCTGTTCAACTTGGAAAACAACTGGTTGAAAGGAACATTGACTTGGTCTATGGAGGAGGGAGCATTGGTTTGATGGGTCAGGTCTCCCAAGCTGTTTATGATGGTGGTCGCCACGTGTTGGGAGTAATTCCCAGAACTCTCATGCCAAGAGAGATCACTGGAGAGCCTGTGGGAGAAGTAAGAGCTGTATCTGGTATGCACCAGCGCAAAGCGGAAATGGCTCGCCAAGCTGATGCCTTCGTTGCCTTGCCAGGTGGCTATGGTACCTTAGAAGAACTCTTGGAAGTGATCACTTGGGCTCAGTTGGGAATTCATGAGAAACCAGTGAGTTACAGTCTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lysine_decarbox

Protein Analysis

153

Amino Acids

16.68

Weight (kDa)

8.6

Isoelectric Point (pI)

41.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LDcluster4 PF18306 28 - 134 2.3e-13 SLOG cluster4 family
Lysine_decarbox PF03641 65 - 149 5.6e-32 Possible lysine decarboxylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 383
AccB1I GGYRCC 1 cut(s) 383
AccB7I CCANNNNNTGG 1 cut(s) 238
AciI CCGC 1 cut(s) 332
AcsI RAATTY 1 cut(s) 429
AcvI CACGTG 1 cut(s) 235
AfaI GTAC 1 cut(s) 385
AfiI CCNNNNNNNGG 1 cut(s) 238
AflIII ACRYGT 1 cut(s) 234
AgsI TTSAA 5 cut(s) 14, 54, 61, 128, 149
AjnI CCWGG 1 cut(s) 370
AjuI GAANNNNNNNTTGG 2 cut(s) 146, 178
AluBI AGCT 4 cut(s) 115, 212, 305, 350
AluI AGCT 4 cut(s) 115, 212, 305, 350
Alw26I GTCTC 1 cut(s) 208
ApeKI GCWGC 1 cut(s) 119
ApoI RAATTY 1 cut(s) 429
Asp718I GGTACC 1 cut(s) 383
AspLEI GCGC 1 cut(s) 327
BanI GGYRCC 1 cut(s) 383
BanII GRGCYC 1 cut(s) 421
BbrPI CACGTG 1 cut(s) 235
BbvI GCAGC 1 cut(s) 106
BccI CCATC 2 cut(s) 187, 215
BciT130I CCWGG 1 cut(s) 372
BclI TGATCA 1 cut(s) 408
BcoDI GTCTC 1 cut(s) 208
BisI GCNGC 1 cut(s) 120
BlsI GCNGC 1 cut(s) 121
Bme1390I CCNGG 1 cut(s) 372
BmiI GGNNCC 1 cut(s) 385
BmrFI CCNGG 1 cut(s) 372
BmsI GCATC 1 cut(s) 343
BpmI CTGGAG 1 cut(s) 300
BsaAI YACGTR 1 cut(s) 235
BsaI GGTCTC 1 cut(s) 208
BsaXI ACNNNNNCTCC 4 cut(s) 172, 202, 273, 303
Bsc4I CCNNNNNNNGG 1 cut(s) 238
Bse1I ACTGG 3 cut(s) 147, 283, 443
BseBI CCWGG 1 cut(s) 372
BseLI CCNNNNNNNGG 1 cut(s) 238
BseMII CTCAG 1 cut(s) 434
BseNI ACTGG 3 cut(s) 147, 283, 443
BseRI GAGGAG 1 cut(s) 189
BseXI GCAGC 1 cut(s) 106
BshNI GGYRCC 1 cut(s) 383
BslI CCNNNNNNNGG 1 cut(s) 238
BsmAI GTCTC 1 cut(s) 208
Bso31I GGTCTC 1 cut(s) 208
Bsp1286I GDGCHC 1 cut(s) 421
Bsp143I GATC 2 cut(s) 273, 408
BspACI CCGC 1 cut(s) 332
BspCNI CTCAG 1 cut(s) 433
BspHI TCATGA 1 cut(s) 433
BspLI GGNNCC 1 cut(s) 385
BspT107I GGYRCC 1 cut(s) 383
BspTNI GGTCTC 1 cut(s) 208
BsrI ACTGG 3 cut(s) 147, 283, 443
BssMI GATC 2 cut(s) 273, 408
Bst2UI CCWGG 1 cut(s) 372
Bst4CI ACNGT 1 cut(s) 455
BstBAI YACGTR 1 cut(s) 235
BstC8I GCNNGC 2 cut(s) 117, 343
BstDEI CTNAG 2 cut(s) 388, 420
BstHHI GCGC 1 cut(s) 327
BstKTI GATC 2 cut(s) 276, 411
BstMAI GTCTC 1 cut(s) 208
BstMBI GATC 2 cut(s) 273, 408
BstMWI GCNNNNNNNGC 3 cut(s) 338, 347, 362
BstNI CCWGG 1 cut(s) 372
BstSCI CCNGG 1 cut(s) 370
BstV1I GCAGC 1 cut(s) 106
BtsI GCAGTG 1 cut(s) 94
BtsIMutI CAGTG 3 cut(s) 94, 276, 450
Cac8I GCNNGC 2 cut(s) 117, 343
CciI TCATGA 1 cut(s) 433
CfoI GCGC 1 cut(s) 327
Csp6I GTAC 1 cut(s) 384
CviAII CATG 2 cut(s) 262, 434
CviJI RGCY 8 cut(s) 115, 212, 286, 305, 341, 350, 378, 419
CviKI_1 RGCY 8 cut(s) 115, 212, 286, 305, 341, 350, 378, 419
CviQI GTAC 1 cut(s) 384
DdeI CTNAG 2 cut(s) 388, 420
DpnI GATC 2 cut(s) 275, 410
DpnII GATC 2 cut(s) 273, 408
Eco24I GRGCYC 1 cut(s) 421
Eco31I GGTCTC 1 cut(s) 208
Eco72I CACGTG 1 cut(s) 235
EcoRI GAATTC 1 cut(s) 429
EcoRII CCWGG 1 cut(s) 370
EcoT38I GRGCYC 1 cut(s) 421
FaeI CATG 2 cut(s) 265, 437
FaiI YATR 6 cut(s) 171, 219, 263, 317, 381, 435
FatI CATG 2 cut(s) 261, 433
FbaI TGATCA 1 cut(s) 408
Fnu4HI GCNGC 1 cut(s) 120
FriOI GRGCYC 1 cut(s) 421
Fsp4HI GCNGC 1 cut(s) 120
GlaI GCGC 1 cut(s) 326
GluI GCNGC 1 cut(s) 120
GsuI CTGGAG 1 cut(s) 300
HhaI GCGC 1 cut(s) 327
Hin1II CATG 2 cut(s) 265, 437
Hin6I GCGC 1 cut(s) 325
HinP1I GCGC 1 cut(s) 325
HinfI GANTC 2 cut(s) 10, 57
Hpy188III TCNNGA 3 cut(s) 14, 54, 434
HpyAV CCTTC 1 cut(s) 367
HpyCH4III ACNGT 1 cut(s) 455
HpyCH4IV ACGT 1 cut(s) 234
HpyCH4V TGCA 1 cut(s) 319
HpyF10VI GCNNNNNNNGC 3 cut(s) 338, 347, 362
HpyF3I CTNAG 2 cut(s) 388, 420
HpySE526I ACGT 1 cut(s) 234
Hsp92II CATG 2 cut(s) 265, 437
HspAI GCGC 1 cut(s) 325
KpnI GGTACC 1 cut(s) 387
Ksp22I TGATCA 1 cut(s) 408
Kzo9I GATC 2 cut(s) 273, 408
LmnI GCTCC 1 cut(s) 180
Lsp1109I GCAGC 1 cut(s) 106
LweI GCATC 1 cut(s) 343
MaeII ACGT 1 cut(s) 234
MaeIII GTNAC 1 cut(s) 449
MalI GATC 2 cut(s) 275, 410
MboI GATC 2 cut(s) 273, 408
MboII GAAGA 2 cut(s) 42, 404
MhlI GDGCHC 1 cut(s) 421
MluCI AATT 2 cut(s) 246, 429
MnlI CCTC 2 cut(s) 167, 170
MspR9I CCNGG 1 cut(s) 372
MvaI CCWGG 1 cut(s) 372
MwoI GCNNNNNNNGC 3 cut(s) 338, 347, 362
NdeII GATC 2 cut(s) 273, 408
NlaIII CATG 2 cut(s) 265, 437
NlaIV GGNNCC 1 cut(s) 385
PagI TCATGA 1 cut(s) 433
PfeI GAWTC 2 cut(s) 10, 57
PflFI GACNNNGTC 1 cut(s) 164
PflMI CCANNNNNTGG 1 cut(s) 238
PkrI GCNGC 1 cut(s) 121
PmaCI CACGTG 1 cut(s) 235
PmlI CACGTG 1 cut(s) 235
Ppu21I YACGTR 1 cut(s) 235
Psp6I CCWGG 1 cut(s) 370
PspCI CACGTG 1 cut(s) 235
PspGI CCWGG 1 cut(s) 370
PspN4I GGNNCC 1 cut(s) 385
PsyI GACNNNGTC 1 cut(s) 164
RsaI GTAC 1 cut(s) 385
RsaNI GTAC 1 cut(s) 384
SatI GCNGC 1 cut(s) 120
Sau3AI GATC 2 cut(s) 273, 408
ScrFI CCNGG 1 cut(s) 372
SduI GDGCHC 1 cut(s) 421
SetI ASST 8 cut(s) 117, 204, 214, 237, 307, 352, 376, 389
SfaNI GCATC 1 cut(s) 343
Sse9I AATT 2 cut(s) 246, 429
SsiI CCGC 1 cut(s) 332
StyD4I CCNGG 1 cut(s) 370
TaaI ACNGT 1 cut(s) 455
TaiI ACGT 1 cut(s) 237
TasI AATT 2 cut(s) 246, 429
TfiI GAWTC 2 cut(s) 10, 57
TscAI CASTG 3 cut(s) 94, 283, 450
TseI GCWGC 1 cut(s) 119
TspDTI ATGAA 2 cut(s) 59, 422
TspRI CASTG 3 cut(s) 94, 283, 450
Tth111I GACNNNGTC 1 cut(s) 164
Van91I CCANNNNNTGG 1 cut(s) 238
XapI RAATTY 1 cut(s) 429
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.