pycom08g01300
NAC Family

Cytokinin-activating enzyme working in the direct activation pathway. Phosphoribohydrolase that converts inactive cytokinin nucleotides to the biologically active free-base forms

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0001203
Physical Location & Seq
Reverse (-)
955993 .. 957810
1818 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g01300.1

Sequence Viewer

Length: 651 bp
ATGGAAAATCAGCAGCAGATTGCAAAGGAATCGAGATTCAGGCGTATCTGCGTTTTCTGTGGCAGCAGTGCTGGCAAGAACCCGAGCTACCAGCTTGCTGCCGTTCAACTCGGCAAAGAATTGGTTGAAAGACACATTGACTTGGTTTATGGAGGAGGAAGTATTGGGTTAATGGGTCAGATCTCCCAAGTTGTCAATGATGGTGGCCGCCACGTGTTGGGAGTAATTCCCACTACCCTCATGCCAATAGAGATCACAGGAGAGCCAGTGGGAGAAATAAGAGTTGTATCAAGCATGCACCAACGCAAAGCGGAAATGGCTCGCCAAGCTGATGCATTCATTGCCTTGCCAGGTGGCTATGGGACCTTGGAAGAACTGTTGGAAATGATCACTTGGGCACAGTTAGGAATCCATGATAAGCCGGTGGGGTTGTTAAATGTGGATGGATACTACAACTCACTGCTATCGTTCGTGGACAAGGCAGTGGACGAAGGTTTCATTGACCCTGCAGCTCGCCACATTATTGTCTCTGCCGAAACTCCCTGCGAACTCATGTGCAAACTAGAGGAATATGCTCCAAAGCATTCTGGGCTGAGTTGGGAGATGGAGCAACAACTGGAATACAAGGCAAAGTCAGACATTGCACGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lysine_decarbox

Protein Analysis

217

Amino Acids

23.76

Weight (kDa)

5.27

Isoelectric Point (pI)

38.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LDcluster4 PF18306 21 - 128 5.6e-12 SLOG cluster4 family
Lysine_decarbox PF03641 58 - 187 1.9e-45 Possible lysine decarboxylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 217
AciI CCGC 2 cut(s) 208, 311
AcoI YGGCCR 1 cut(s) 205
AcvI CACGTG 1 cut(s) 214
AfiI CCNNNNNNNGG 1 cut(s) 217
AflIII ACRYGT 1 cut(s) 213
AgsI TTSAA 2 cut(s) 107, 128
AjnI CCWGG 1 cut(s) 349
AluBI AGCT 4 cut(s) 87, 94, 329, 512
AluI AGCT 4 cut(s) 87, 94, 329, 512
Alw26I GTCTC 1 cut(s) 532
Ama87I CYCGRG 1 cut(s) 82
AoxI GGCC 1 cut(s) 205
ApeKI GCWGC 4 cut(s) 13, 63, 98, 509
AspS9I GGNCC 1 cut(s) 363
AvaI CYCGRG 1 cut(s) 82
AvaII GGWCC 1 cut(s) 363
BaeGI GKGCMC 1 cut(s) 400
BbrPI CACGTG 1 cut(s) 214
BbvI GCAGC 4 cut(s) 25, 75, 85, 521
BccI CCATC 3 cut(s) 194, 437, 598
BceAI ACGGC 1 cut(s) 86
BcgI CGANNNNNNTGC 2 cut(s) 12, 46
BciT130I CCWGG 1 cut(s) 351
BciVI GTATCC 1 cut(s) 440
BclI TGATCA 1 cut(s) 387
BcoDI GTCTC 1 cut(s) 532
BfaI CTAG 1 cut(s) 563
BfmI CTRYAG 1 cut(s) 507
BfuI GTATCC 1 cut(s) 440
BglII AGATCT 1 cut(s) 180
BisI GCNGC 5 cut(s) 14, 64, 99, 208, 510
BlsI GCNGC 5 cut(s) 15, 65, 100, 209, 511
Bme1390I CCNGG 1 cut(s) 351
Bme18I GGWCC 1 cut(s) 363
BmeT110I CYCGRG 1 cut(s) 82
BmgT120I GGNCC 1 cut(s) 363
BmiI GGNNCC 1 cut(s) 364
BmrFI CCNGG 1 cut(s) 351
BmsI GCATC 1 cut(s) 322
BsaAI YACGTR 1 cut(s) 214
BsaJI CCNNGG 1 cut(s) 366
BsaXI ACNNNNNCTCC 2 cut(s) 252, 282
Bsc4I CCNNNNNNNGG 1 cut(s) 217
Bse118I RCCGGY 1 cut(s) 421
Bse1I ACTGG 2 cut(s) 266, 621
Bse3DI GCAATG 2 cut(s) 339, 639
BseBI CCWGG 1 cut(s) 351
BseDI CCNNGG 1 cut(s) 366
BseGI GGATG 1 cut(s) 448
BseLI CCNNNNNNNGG 1 cut(s) 217
BseMI GCAATG 2 cut(s) 339, 639
BseMII CTCAG 1 cut(s) 584
BseNI ACTGG 2 cut(s) 266, 621
BseRI GAGGAG 1 cut(s) 168
BseSI GKGCMC 1 cut(s) 400
BseXI GCAGC 4 cut(s) 25, 75, 85, 521
BshFI GGCC 1 cut(s) 207
BsiHKCI CYCGRG 1 cut(s) 82
BsiSI CCGG 1 cut(s) 422
BslFI GGGAC 1 cut(s) 376
BslI CCNNNNNNNGG 1 cut(s) 217
BsmAI GTCTC 1 cut(s) 532
BsmFI GGGAC 1 cut(s) 376
BsmI GAATGC 2 cut(s) 335, 583
BsnI GGCC 1 cut(s) 207
BsoBI CYCGRG 1 cut(s) 82
Bsp1286I GDGCHC 1 cut(s) 400
Bsp143I GATC 3 cut(s) 180, 252, 387
BspACI CCGC 2 cut(s) 208, 311
BspANI GGCC 1 cut(s) 207
BspCNI CTCAG 1 cut(s) 585
BspLI GGNNCC 1 cut(s) 364
BspMAI CTGCAG 1 cut(s) 511
BsrDI GCAATG 2 cut(s) 339, 639
BsrFI RCCGGY 1 cut(s) 421
BsrI ACTGG 2 cut(s) 266, 621
BssAI RCCGGY 1 cut(s) 421
BssECI CCNNGG 1 cut(s) 366
BssMI GATC 3 cut(s) 180, 252, 387
BssT1I CCWWGG 1 cut(s) 366
Bst2UI CCWGG 1 cut(s) 351
Bst4CI ACNGT 2 cut(s) 378, 402
BstAPI GCANNNNNTGC 1 cut(s) 341
BstBAI YACGTR 1 cut(s) 214
BstC8I GCNNGC 5 cut(s) 73, 96, 296, 322, 514
BstDEI CTNAG 1 cut(s) 593
BstF5I GGATG 1 cut(s) 448
BstKTI GATC 3 cut(s) 183, 255, 390
BstMAI GTCTC 1 cut(s) 532
BstMBI GATC 3 cut(s) 180, 252, 387
BstMWI GCNNNNNNNGC 5 cut(s) 72, 317, 326, 341, 589
BstNI CCWGG 1 cut(s) 351
BstNSI RCATGY 1 cut(s) 298
BstSCI CCNGG 1 cut(s) 349
BstSFI CTRYAG 1 cut(s) 507
BstSLI GKGCMC 1 cut(s) 400
BstV1I GCAGC 4 cut(s) 25, 75, 85, 521
BstX2I RGATCY 1 cut(s) 180
BstYI RGATCY 1 cut(s) 180
BsuI GTATCC 1 cut(s) 440
BsuRI GGCC 1 cut(s) 207
BtsCI GGATG 1 cut(s) 448
BtsI GCAGTG 3 cut(s) 73, 458, 489
BtsIMutI CAGTG 4 cut(s) 73, 273, 458, 489
Cac8I GCNNGC 5 cut(s) 73, 96, 296, 322, 514
Cfr10I RCCGGY 1 cut(s) 421
Cfr13I GGNCC 1 cut(s) 363
CspCI CAANNNNNGTGG 2 cut(s) 184, 219
CviAII CATG 4 cut(s) 241, 295, 413, 553
DdeI CTNAG 1 cut(s) 593
DpnI GATC 3 cut(s) 182, 254, 389
DpnII GATC 3 cut(s) 180, 252, 387
EaeI YGGCCR 1 cut(s) 205
Eco130I CCWWGG 1 cut(s) 366
Eco47I GGWCC 1 cut(s) 363
Eco72I CACGTG 1 cut(s) 214
Eco88I CYCGRG 1 cut(s) 82
EcoO109I RGGNCCY 1 cut(s) 363
EcoRII CCWGG 1 cut(s) 349
EcoT14I CCWWGG 1 cut(s) 366
EcoT22I ATGCAT 1 cut(s) 337
ErhI CCWWGG 1 cut(s) 366
FaeI CATG 4 cut(s) 244, 298, 416, 556
FaiI YATR 7 cut(s) 150, 242, 296, 360, 414, 554, 573
FaqI GGGAC 1 cut(s) 376
FatI CATG 4 cut(s) 240, 294, 412, 552
FbaI TGATCA 1 cut(s) 387
Fnu4HI GCNGC 5 cut(s) 14, 64, 99, 208, 510
FokI GGATG 1 cut(s) 455
Fsp4HI GCNGC 5 cut(s) 14, 64, 99, 208, 510
FspBI CTAG 1 cut(s) 563
GluI GCNGC 5 cut(s) 14, 64, 99, 208, 510
HaeIII GGCC 1 cut(s) 207
HapII CCGG 1 cut(s) 422
Hin1II CATG 4 cut(s) 244, 298, 416, 556
HinfI GANTC 3 cut(s) 29, 36, 408
HpaII CCGG 1 cut(s) 422
Hpy166II GTNNAC 2 cut(s) 475, 487
Hpy188I TCNGA 2 cut(s) 180, 637
Hpy188III TCNNGA 1 cut(s) 33
Hpy8I GTNNAC 2 cut(s) 475, 487
HpyAV CCTTC 1 cut(s) 485
HpyCH4III ACNGT 2 cut(s) 378, 402
HpyCH4IV ACGT 2 cut(s) 213, 646
HpyCH4V TGCA 6 cut(s) 23, 298, 335, 509, 558, 644
HpyF10VI GCNNNNNNNGC 5 cut(s) 72, 317, 326, 341, 589
HpyF3I CTNAG 1 cut(s) 593
HpySE526I ACGT 2 cut(s) 213, 646
Hsp92II CATG 4 cut(s) 244, 298, 416, 556
Ksp22I TGATCA 1 cut(s) 387
Kzo9I GATC 3 cut(s) 180, 252, 387
LmnI GCTCC 2 cut(s) 580, 607
Lsp1109I GCAGC 4 cut(s) 25, 75, 85, 521
LweI GCATC 1 cut(s) 322
MaeI CTAG 1 cut(s) 563
MaeII ACGT 2 cut(s) 213, 646
MalI GATC 3 cut(s) 182, 254, 389
MboI GATC 3 cut(s) 180, 252, 387
MboII GAAGA 1 cut(s) 383
MflI RGATCY 1 cut(s) 180
MhlI GDGCHC 1 cut(s) 400
MluCI AATT 2 cut(s) 119, 225
MmeI TCCRAC 1 cut(s) 360
MnlI CCTC 4 cut(s) 146, 149, 248, 559
Mph1103I ATGCAT 1 cut(s) 337
MseI TTAA 2 cut(s) 170, 434
MspI CCGG 1 cut(s) 422
MspR9I CCNGG 1 cut(s) 351
Mva1269I GAATGC 2 cut(s) 335, 583
MvaI CCWGG 1 cut(s) 351
MwoI GCNNNNNNNGC 5 cut(s) 72, 317, 326, 341, 589
NdeII GATC 3 cut(s) 180, 252, 387
NlaIII CATG 4 cut(s) 244, 298, 416, 556
NlaIV GGNNCC 1 cut(s) 364
NmeAIII GCCGAG 1 cut(s) 90
NsiI ATGCAT 1 cut(s) 337
NspI RCATGY 1 cut(s) 298
PaeI GCATGC 1 cut(s) 298
PctI GAATGC 2 cut(s) 335, 583
PfeI GAWTC 3 cut(s) 29, 36, 408
PflMI CCANNNNNTGG 1 cut(s) 217
PkrI GCNGC 5 cut(s) 15, 65, 100, 209, 511
PmaCI CACGTG 1 cut(s) 214
PmlI CACGTG 1 cut(s) 214
Ppu21I YACGTR 1 cut(s) 214
PpuMI RGGWCCY 1 cut(s) 363
Psp5II RGGWCCY 1 cut(s) 363
Psp6I CCWGG 1 cut(s) 349
PspCI CACGTG 1 cut(s) 214
PspGI CCWGG 1 cut(s) 349
PspN4I GGNNCC 1 cut(s) 364
PspPI GGNCC 1 cut(s) 363
PspPPI RGGWCCY 1 cut(s) 363
PsrI GAACNNNNNNTAC 2 cut(s) 71, 103
PstI CTGCAG 1 cut(s) 511
PsuI RGATCY 1 cut(s) 180
SaqAI TTAA 2 cut(s) 170, 434
SatI GCNGC 5 cut(s) 14, 64, 99, 208, 510
Sau3AI GATC 3 cut(s) 180, 252, 387
Sau96I GGNCC 1 cut(s) 363
ScrFI CCNGG 1 cut(s) 351
SduI GDGCHC 1 cut(s) 400
SetI ASST 9 cut(s) 89, 96, 216, 331, 355, 368, 496, 514, 649
SfaNI GCATC 1 cut(s) 322
SfcI CTRYAG 1 cut(s) 507
SinI GGWCC 1 cut(s) 363
SphI GCATGC 1 cut(s) 298
Sse9I AATT 2 cut(s) 119, 225
SsiI CCGC 2 cut(s) 208, 311
SspMI CTAG 1 cut(s) 563
StyD4I CCNGG 1 cut(s) 349
StyI CCWWGG 1 cut(s) 366
TaaI ACNGT 2 cut(s) 378, 402
TaiI ACGT 2 cut(s) 216, 649
TaqI TCGA 1 cut(s) 32
TasI AATT 2 cut(s) 119, 225
TauI GCSGC 1 cut(s) 210
TfiI GAWTC 3 cut(s) 29, 36, 408
Tru1I TTAA 2 cut(s) 170, 434
Tru9I TTAA 2 cut(s) 170, 434
TscAI CASTG 4 cut(s) 73, 273, 465, 489
TseI GCWGC 4 cut(s) 13, 63, 98, 509
TspDTI ATGAA 2 cut(s) 328, 487
TspRI CASTG 4 cut(s) 73, 273, 465, 489
Van91I CCANNNNNTGG 1 cut(s) 217
VpaK11BI GGWCC 1 cut(s) 363
XceI RCATGY 1 cut(s) 298
XspI CTAG 1 cut(s) 563
Zsp2I ATGCAT 1 cut(s) 337
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.