pycom11g00540

Cytokinin riboside 5'-monophosphate phosphoribohydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0001203
Physical Location & Seq
Forward (+)
394164 .. 395466
1303 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom11g00540.2

Sequence Viewer

Length: 504 bp
ATGGGGCCTATATATGTATACTTCTTATTGTTAGAGGGCCTGCGACTTATTCAGCAGAAAGAATTAATGGGTAAGTTCAAAACGGTGTGTGTATTTTGTGGGAGTAATTCCGGGCACAGAAAGATATTCAGTGATGCAGCTCTTCAACTTGGGAAAGAACTGGTGGAGAGGAAGATGGAATTGGTGTATGGAGGAGGAAGTGTAGGGTTGATGGGGTTGGTTTCTCAAACAGTTTATGATGGCGGATGTCATGTTCTTGGAGTAATCCCAACAGCCCTAATTCCACTTGAGATATCAGGCAATTCAGTAGGAGAAGTATTGGTTGTATCGAACATGCATGAAAGAAAGGCTGAGATGGCTCGTCGAGCTGATGCTTTCATTGCATTGCCTGGTGGGTATGGAACCATGGAAGAGTTGCTTGAGATGATAACATGGTCTCAGCTTGGAATCCATAATAAACCAGTAAGTTTAATATATATTAAGTGTTTCCTCTTATTATTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lysine_decarbox

Protein Analysis

168

Amino Acids

18.33

Weight (kDa)

6.89

Isoelectric Point (pI)

29.91

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 18
AciI CCGC 1 cut(s) 243
AgsI TTSAA 2 cut(s) 79, 146
AjnI CCWGG 1 cut(s) 388
AjuI GAANNNNNNNTTGG 2 cut(s) 164, 196
AloI GAACNNNNNNTCC 2 cut(s) 237, 269
AluBI AGCT 3 cut(s) 140, 368, 442
AluI AGCT 3 cut(s) 140, 368, 442
Alw26I GTCTC 1 cut(s) 441
AoxI GGCC 2 cut(s) 5, 37
ApeKI GCWGC 1 cut(s) 137
AseI ATTAAT 1 cut(s) 65
AspS9I GGNCC 2 cut(s) 5, 37
AsuC2I CCSGG 1 cut(s) 112
BaeGI GKGCMC 1 cut(s) 117
BbvI GCAGC 1 cut(s) 149
BccI CCATC 4 cut(s) 169, 205, 233, 349
BciT130I CCWGG 1 cut(s) 390
BcnI CCSGG 1 cut(s) 112
BcoDI GTCTC 1 cut(s) 441
BisI GCNGC 1 cut(s) 138
BlsI GCNGC 1 cut(s) 139
Bme1390I CCNGG 2 cut(s) 112, 390
BmgT120I GGNCC 2 cut(s) 5, 37
BmiI GGNNCC 2 cut(s) 6, 403
BmrFI CCNGG 2 cut(s) 112, 390
BmsI GCATC 2 cut(s) 124, 361
BpuEI CTTGAG 2 cut(s) 308, 440
BpuMI CCSGG 1 cut(s) 112
BsaI GGTCTC 1 cut(s) 441
BsaJI CCNNGG 1 cut(s) 405
BsaXI ACNNNNNCTCC 2 cut(s) 186, 216
Bse1I ACTGG 2 cut(s) 165, 461
Bse3DI GCAATG 2 cut(s) 378, 383
BseBI CCWGG 1 cut(s) 390
BseDI CCNNGG 1 cut(s) 405
BseGI GGATG 1 cut(s) 251
BseMI GCAATG 2 cut(s) 378, 383
BseMII CTCAG 2 cut(s) 342, 452
BseNI ACTGG 2 cut(s) 165, 461
BseRI GAGGAG 1 cut(s) 207
BseSI GKGCMC 1 cut(s) 117
BseXI GCAGC 1 cut(s) 149
BshFI GGCC 2 cut(s) 7, 39
BsiSI CCGG 1 cut(s) 111
BsmAI GTCTC 1 cut(s) 441
BsnI GGCC 2 cut(s) 7, 39
Bso31I GGTCTC 1 cut(s) 441
Bsp1286I GDGCHC 1 cut(s) 117
Bsp19I CCATGG 1 cut(s) 405
BspACI CCGC 1 cut(s) 243
BspANI GGCC 2 cut(s) 7, 39
BspCNI CTCAG 2 cut(s) 343, 451
BspLI GGNNCC 2 cut(s) 6, 403
BspQI GCTCTTC 1 cut(s) 147
BspTNI GGTCTC 1 cut(s) 441
BsrDI GCAATG 2 cut(s) 378, 383
BsrI ACTGG 2 cut(s) 165, 461
BssECI CCNNGG 1 cut(s) 405
BssNAI GTATAC 1 cut(s) 19
BssT1I CCWWGG 1 cut(s) 405
Bst1107I GTATAC 1 cut(s) 19
Bst2UI CCWGG 1 cut(s) 390
Bst4CI ACNGT 2 cut(s) 85, 232
Bst6I CTCTTC 2 cut(s) 147, 405
BstC8I GCNNGC 1 cut(s) 41
BstDEI CTNAG 2 cut(s) 351, 438
BstDSI CCRYGG 1 cut(s) 405
BstF5I GGATG 1 cut(s) 251
BstMAI GTCTC 1 cut(s) 441
BstMWI GCNNNNNNNGC 3 cut(s) 356, 365, 380
BstNI CCWGG 1 cut(s) 390
BstNSI RCATGY 1 cut(s) 337
BstSCI CCNGG 2 cut(s) 110, 388
BstSLI GKGCMC 1 cut(s) 117
BstV1I GCAGC 1 cut(s) 149
BstZ17I GTATAC 1 cut(s) 19
BsuRI GGCC 2 cut(s) 7, 39
BtgI CCRYGG 1 cut(s) 405
BtsCI GGATG 1 cut(s) 251
BtsIMutI CAGTG 1 cut(s) 136
Cac8I GCNNGC 1 cut(s) 41
Cfr13I GGNCC 2 cut(s) 5, 37
CviAII CATG 5 cut(s) 251, 334, 338, 406, 432
CviJI RGCY 8 cut(s) 7, 39, 140, 275, 350, 359, 368, 442
CviKI_1 RGCY 8 cut(s) 7, 39, 140, 275, 350, 359, 368, 442
DdeI CTNAG 2 cut(s) 351, 438
Eam1104I CTCTTC 2 cut(s) 147, 405
EarI CTCTTC 2 cut(s) 147, 405
EciI GGCGGA 1 cut(s) 258
Eco130I CCWWGG 1 cut(s) 405
Eco31I GGTCTC 1 cut(s) 441
Eco32I GATATC 1 cut(s) 294
EcoO109I RGGNCCY 2 cut(s) 5, 37
EcoRII CCWGG 1 cut(s) 388
EcoRV GATATC 1 cut(s) 294
EcoT14I CCWWGG 1 cut(s) 405
EcoT22I ATGCAT 1 cut(s) 339
ErhI CCWWGG 1 cut(s) 405
FaeI CATG 5 cut(s) 254, 337, 341, 409, 435
FalI AAGNNNNNCTT 2 cut(s) 402, 434
FatI CATG 5 cut(s) 250, 333, 337, 405, 431
FblI GTMKAC 1 cut(s) 18
Fnu4HI GCNGC 1 cut(s) 138
FokI GGATG 1 cut(s) 258
Fsp4HI GCNGC 1 cut(s) 138
GluI GCNGC 1 cut(s) 138
HaeIII GGCC 2 cut(s) 7, 39
HapII CCGG 1 cut(s) 111
Hin1II CATG 5 cut(s) 254, 337, 341, 409, 435
HinfI GANTC 1 cut(s) 447
HpaII CCGG 1 cut(s) 111
Hpy166II GTNNAC 1 cut(s) 19
Hpy8I GTNNAC 1 cut(s) 19
Hpy99I CGWCG 1 cut(s) 366
HpyCH4III ACNGT 2 cut(s) 85, 232
HpyCH4V TGCA 3 cut(s) 137, 337, 383
HpyF10VI GCNNNNNNNGC 3 cut(s) 356, 365, 380
HpyF3I CTNAG 2 cut(s) 351, 438
Hsp92II CATG 5 cut(s) 254, 337, 341, 409, 435
LguI GCTCTTC 1 cut(s) 147
LpnPI CCDG 7 cut(s) 53, 124, 146, 282, 375, 402, 474
Lsp1109I GCAGC 1 cut(s) 149
LweI GCATC 2 cut(s) 124, 361
MboII GAAGA 3 cut(s) 134, 184, 422
MhlI GDGCHC 1 cut(s) 117
MluCI AATT 5 cut(s) 62, 106, 179, 279, 301
MnlI CCTC 5 cut(s) 28, 162, 185, 188, 500
Mph1103I ATGCAT 1 cut(s) 339
MseI TTAA 3 cut(s) 65, 470, 480
MspI CCGG 1 cut(s) 111
MspR9I CCNGG 2 cut(s) 112, 390
MvaI CCWGG 1 cut(s) 390
MwoI GCNNNNNNNGC 3 cut(s) 356, 365, 380
NciI CCSGG 1 cut(s) 112
NcoI CCATGG 1 cut(s) 405
NlaIII CATG 5 cut(s) 254, 337, 341, 409, 435
NlaIV GGNNCC 2 cut(s) 6, 403
NsiI ATGCAT 1 cut(s) 339
NspI RCATGY 1 cut(s) 337
PciSI GCTCTTC 1 cut(s) 147
PfeI GAWTC 1 cut(s) 447
PkrI GCNGC 1 cut(s) 139
PshBI ATTAAT 1 cut(s) 65
Psp6I CCWGG 1 cut(s) 388
PspGI CCWGG 1 cut(s) 388
PspN4I GGNNCC 2 cut(s) 6, 403
PspPI GGNCC 2 cut(s) 5, 37
SapI GCTCTTC 1 cut(s) 147
SaqAI TTAA 3 cut(s) 65, 470, 480
SatI GCNGC 1 cut(s) 138
Sau96I GGNCC 2 cut(s) 5, 37
ScrFI CCNGG 2 cut(s) 112, 390
SduI GDGCHC 1 cut(s) 117
SetI ASST 3 cut(s) 142, 370, 444
SfaNI GCATC 2 cut(s) 124, 361
SmlI CTYRAG 2 cut(s) 287, 419
SmoI CTYRAG 2 cut(s) 287, 419
Sse9I AATT 5 cut(s) 62, 106, 179, 279, 301
SsiI CCGC 1 cut(s) 243
StyD4I CCNGG 2 cut(s) 110, 388
StyI CCWWGG 1 cut(s) 405
TaaI ACNGT 2 cut(s) 85, 232
TaqI TCGA 2 cut(s) 329, 364
TasI AATT 5 cut(s) 62, 106, 179, 279, 301
TfiI GAWTC 1 cut(s) 447
Tru1I TTAA 3 cut(s) 65, 470, 480
Tru9I TTAA 3 cut(s) 65, 470, 480
TscAI CASTG 1 cut(s) 136
TseI GCWGC 1 cut(s) 137
TspDTI ATGAA 2 cut(s) 354, 367
TspRI CASTG 1 cut(s) 136
VspI ATTAAT 1 cut(s) 65
XceI RCATGY 1 cut(s) 337
XmiI GTMKAC 1 cut(s) 18
Zsp2I ATGCAT 1 cut(s) 339
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.