Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0001672

Physical Location & Seq

Genomic Coordinates Reverse (-)

9745947 .. 9760152

Gene Structure 14206 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1080100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2568 bp

ATGGCCACCGTATCCCGGCCACCGTTTTTCAACCCGACCCGCCCCAAGAACCAAATTGTCTGCGGAAAACCCAACAACGGCGACTCCTCCGCCTTCCAAGAGAAGAAGCAGGTCTCCGTGGACTACGACCACGGCACTCACCACGTCTCCACCCGAATCACCGGCCTCACCAAACAACACATCCCCAAACGCCATCGTTTACGAGTCGAAACCGAGCGTTTCCAGAAGGACTGGGCCGTCTCCGACGTCGTCGACCGCCTCTACAAGCTCCGCCACTGGGAGGACATCGAGGGCTTACTCAATCAATGGGTCGGGCGCTTTGCTCGTAAGAACTTCCCTGTTCTCATCAAGGAAATGACAGAGAGGGGTTCGGTGGAACACTGTGTGCGAGTGTTCGGGTGGATGAAGAACCAGAAGAACTATTGTGCTCGCACTGACATGTATAATATGATGATCAGATTGCACGCTCGGCATAATCTTGTCGATAAAGCTCGCGGCTTGTTTTTCGAAATGCAAGAGTGGAGGTGCAAGCCTGATGCTGAGTCCTACAATGCTCTTATCAATGCACATGGTCGAGCAGGTCAATGGCGTTGGGCCATGAATCTTATGGATGACATGTTGCGTGCAGCTATCCCTCCTAGTAGATCAACATATAACAACTTGATCAATGCTTGTGGATCTAGTGGAAATTGGAGGGAAGCTTTGAAAGTATGCAAGAAAATGACAGATAATGGAGTTGGGCCTGATCTTGTGACTCACAATATTGTTTTATCTGCATACAAAACTGGGGCTCAGTATTCAAAGGCTTTATCTTATTTTGAACTTATGAAGGGAACTAATATCCGTCCAGACACAACCACCCTTAATATTGTTATATATTGCCTTGTAAAGCTTGGACAATATGGGAAAGCCATTGATATTTTCAGCTCTATGAGAGATAAGAGAGCTGAATGCCGTCCTGACATTGTGACATTCACCAGCATCATTCATCTGTATTCTATGTCCGGGCAGATAGAAAATTGTACAGCTGTATTCAGTACAATACTTGCAGAAGGCCTAAAACCTAACATTGTTTCATACAATGCACTATTGGGTGCATATGCTTCACATGGGATGAGCGCAGAGGCATTGTCAGTTTTTAATGAGATAAAGAAAAGTAGTTTCCGGCCAGATGTTGTGTCGTATACATCCCTACTCAATTCATACGGAAGATCACAACAACCTCAAAAGGCCAGGGCAGTTTTTGACATGATGAAGAGAAACAACTTGAAGCCAAATCTTGTGAGCTATAACGCACTGATTAATGCCTATGGATCTAATGGTTTATTAGCTGAAGCGGTAGAAGTCTTGCGTGAGATGGAGCGAGATGGAATTCATCCAAACATTGTCTCAATATGTACACTTTTGGCAGCTTGTGGACAATGCGGTCAGAAGGTGAAAATTGATGCCGTGCTTTCAGCAGCGAAGCTGCGAGGCATTGAATTGAACACAATTGCATTTAATTCAGCTATTGGAAGCTATATGAATTTGGGAGAACATGAGAAAGCTATAAACTTGTATCAATCTATGAGAAAGAAGAAAGTTAAACCGGATTCTGTTACATACACTGTGTTGATAAGTGGCTGTTGTAAGATTTCAAAACACAGTGAGGCAATAACATTTTTTGATGAAATGGTGGATTTGAAAATTCTTGTTTCCAAAGAGGTCTACTCATCTGTGATCTCTGCCTACAGCAAGATGGGCCAACTTACAGAAGCAGAATCTATATTCAACTTGATGAAAACGGCTGGTTGTGCTCCTGATGTAGTTACATATACTACAATGCTGCATGCGTATAGTGCTGCTGAGAATTGGGAAAAGGCTTGTGTTCTATTCCAAGAAATGGAAACTAATGGCATCCAACTAGATGTCATTGCATGCTCAGCTTTAATGAGAGCTTTTAACAAAGGAGGCAACCCATCCAGGGTTCTTATTCTGGCTGAAATCATGCGAGAAAAAGAAATTCCTTTCAGTGATGCCATTTTCTTTGAAATGGTATCAGCCTGTAGCTTATTACAAGATTGGAGAACAACAATGGAATTGATTAAGTTGATTGAGCCCTCACTACCTGGAGTTTCAATTGGACTTGTGAATCAACTTCTGCGTATTCTTGGAAGAAGTGGAAAGATTGAAACTATGATGAAGTTATTCTACAAGATAGTGGCATCCAGTGGTGATATCAATTTTGATACTTATTCAATTTTGTTGAAGAATCTCCTGTCTGTCGGAAATTGGAGAAAATATTTTGAGGTACTACAATGGATGGTGGATGCTGGAATTCGACCGTCAAGCCAAATGTACCTTGATATATCATCTTTTGCACACAAAAGCGCTGGAGCTGAATATGCTACTCTTATAAAAGAAAGAATTGAACTTTTAAAAAGAAAATCTGGGTACCAAGATTTGACAAGCGAGCCCTGCGATACACATTCCACCTCTTCGCTGATAAGTAGAACGAAGGAAGAGAACCACAGGCTGCTGGAGTCAACAATGGCTGGTACATATGCAGTTGCCGAGTACCATCTGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003676 GO:0003723 GO:0003735 GO:0003824 GO:0004518 GO:0004519 GO:0005198 GO:0005488 GO:0006139 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0008380 GO:0009451 GO:0009790 GO:0009791 GO:0009793 GO:0009987 GO:0010154 GO:0010239 GO:0010467 GO:0016070 GO:0016071 GO:0016787 GO:0016788 GO:0022414 GO:0031425 GO:0032501 GO:0032502 GO:0034641 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0046483 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0061458 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_2 PPR_3

Protein Analysis

856

Amino Acids

96.31

Weight (kDa)

9.01

Isoelectric Point (pI)

35.51

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	147 - 188	1.7e-09	PPR repeat family
PPR_1	PF12854	175 - 206	2.9e-06	PPR repeat
PPR_2	PF13041	178 - 225	1.7e-11	PPR repeat family
PPR_long	PF17177	200 - 313	1.9e-07	Pentacotripeptide-repeat region of PRORP
PPR_3	PF13812	202 - 260	8.4e-09	Pentatricopeptide repeat domain
TPR_24	PF23276	212 - 310	1.5e-08	Fungal tetratrico peptide repeats
PPR_2	PF13041	213 - 260	6.9e-11	PPR repeat family
PPR	PF01535	216 - 246	1.7e-07	PPR repeat
PPR_2	PF13041	283 - 330	7.2e-08	PPR repeat family
PPR_3	PF13812	315 - 367	7.3e-10	Pentatricopeptide repeat domain
PPR_2	PF13041	355 - 402	9.4e-14	PPR repeat family
PPR	PF01535	358 - 387	2.5e-06	PPR repeat
PPR_long	PF17177	374 - 486	3.4e-09	Pentacotripeptide-repeat region of PRORP
PPR_3	PF13812	378 - 440	9.3e-15	Pentatricopeptide repeat domain
PPR_2	PF13041	390 - 436	6.5e-13	PPR repeat family
TPR_24	PF23276	398 - 523	3.9e-12	Fungal tetratrico peptide repeats
PPR_1	PF12854	422 - 454	1.8e-10	PPR repeat
PPR_2	PF13041	425 - 472	6.8e-12	PPR repeat family
PPR	PF01535	428 - 458	4.2e-09	PPR repeat
PPR_3	PF13812	485 - 542	7e-08	Pentatricopeptide repeat domain
PPR_2	PF13041	496 - 544	1.6e-16	PPR repeat family
PPR_long	PF17177	514 - 657	3e-14	Pentacotripeptide-repeat region of PRORP
PPR_1	PF12854	527 - 559	1e-08	PPR repeat
PPR	PF01535	533 - 561	6.8e-07	PPR repeat
PPR_3	PF13812	555 - 612	1.1e-07	Pentatricopeptide repeat domain
TPR_24	PF23276	565 - 652	1.8e-06	Fungal tetratrico peptide repeats
PPR	PF01535	569 - 598	3.6e-07	PPR repeat
PPR_2	PF13041	569 - 612	1.7e-12	PPR repeat family
PPR_1	PF12854	597 - 629	9.8e-08	PPR repeat
PPR_2	PF13041	600 - 648	7.7e-12	PPR repeat family
PPR	PF01535	603 - 633	6.7e-07	PPR repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0001672)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G41720 AT2G41720 AT2G41720
fragaria_vesca	FvH4_6g24060 FvH4_6g24060 FvH4_6g24060 FvH4_6g24060 FvH4_6g24060 FvH4_6g24060 FvH4_6g24060 FvH4_6g24060 FvH4_6g24060 FvH4_6g24060
malus_domestica	MD12G1080100.v1.1
prunus_persica	Prupe.7G058400_v2.0.a1 Prupe.7G058400_v2.0.a1 Prupe.7G058400_v2.0.a1
rosa_chinensis	RchiOBHm_Chr3g0482651
rosa_laevigata	RLG00000001090 RLG00000016982 RLG00000023335 RLG00000028152 RLG00000032501
rosa_multiflora	Rmu_sc0004946.1_g000004 Rmu_sc0006069.1_g000004 Rmu_sc0015893.1_g000010
rosa_roxburghii	Rroxscaffold_3G00229260 Rroxscaffold_3G00244090 Rroxscaffold_4G00320230 Rroxscaffold_6G00399060 Rroxscaffold_7G00168600
rosa_rugosa	Rorug03G0197600 Rorug03G0197700
rosa_samantha	Rh3AG051200 Rh3AG249000 Rh3BG052500 Rh3BG284300 Rh3CG052100 Rh3CG282200 Rh3DG052800 Rh3DG278200 Rh4BG176200 Rh6AG132300 Rh7AG450400 Rh7BG246700 Rh7BG421800 Rh7BG421900 Rh7CG470100
rosa_wichuraiana	Rw3G022490

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2396
AasI	GACNNNNNNGTC	2 cut(s)	236, 1425
AatII	GACGTC	1 cut(s)	249
Acc36I	ACCTGC	2 cut(s)	100, 569
Acc65I	GGTACC	1 cut(s)	2433
AccB1I	GGYRCC	1 cut(s)	2433
AccB7I	CCANNNNNTGG	1 cut(s)	1882
AccI	GTMKAC	3 cut(s)	252, 1184, 1707
AccII	CGCG	1 cut(s)	495
AciI	CCGC	8 cut(s)	40, 63, 90, 256, 271, 495, 1337, 1425
AclWI	GGATC	2 cut(s)	685, 1321
AcoI	YGGCCR	3 cut(s)	3, 17, 1166
AcsI	RAATTY	5 cut(s)	1371, 1525, 1686, 2001, 2316
AcuI	CTGAAG	1 cut(s)	1353
AcyI	GRCGYC	1 cut(s)	246
AdeI	CACNNNGTG	1 cut(s)	385
AfaI	GTAC	8 cut(s)	1024, 1039, 1399, 2292, 2339, 2435, 2539, 2558
AfeI	AGCGCT	1 cut(s)	2371
AfiI	CCNNNNNNNGG	6 cut(s)	15, 77, 277, 280, 1882, 1963
AflIII	ACRYGT	2 cut(s)	438, 615
AjiI	CACGTC	1 cut(s)	145
AjnI	CCWGG	3 cut(s)	1232, 1961, 2107
Alw21I	GWGCWC	2 cut(s)	430, 1798
Alw26I	GTCTC	4 cut(s)	118, 151, 244, 1393
AlwI	GGATC	2 cut(s)	685, 1321
Aor51HI	AGCGCT	1 cut(s)	2371
ApeKI	GCWGC	7 cut(s)	626, 1409, 1460, 1468, 1825, 1841, 2515
ApoI	RAATTY	5 cut(s)	1371, 1525, 1686, 2001, 2316
AseI	ATTAAT	1 cut(s)	1302
Asp700I	GAANNNNTTC	1 cut(s)	2186
Asp718I	GGTACC	1 cut(s)	2433
AspLEI	GCGC	3 cut(s)	318, 1121, 2372
AspS9I	GGNCC	4 cut(s)	234, 594, 740, 1741
AsuC2I	CCSGG	2 cut(s)	16, 1006
AsuHPI	GGTGA	6 cut(s)	131, 151, 160, 967, 1447, 2225
AsuII	TTCGAA	1 cut(s)	507
BalI	TGGCCA	1 cut(s)	5
BanI	GGYRCC	1 cut(s)	2433
BanII	GRGCYC	3 cut(s)	793, 2100, 2457
BarI	GAAGNNNNNNTAC	2 cut(s)	2174, 2206
Bbv12I	GWGCWC	2 cut(s)	430, 1798
BbvI	GCAGC	7 cut(s)	638, 1421, 1455, 1472, 1812, 1828, 2502
BccI	CCATC	6 cut(s)	201, 1351, 1361, 1732, 1966, 2296
BceAI	ACGGC	6 cut(s)	94, 148, 221, 939, 1433, 1800
BciT130I	CCWGG	3 cut(s)	1234, 1963, 2109
BciVI	GTATCC	1 cut(s)	22
BclI	TGATCA	2 cut(s)	453, 663
BcnI	CCSGG	2 cut(s)	16, 1006
BcoDI	GTCTC	4 cut(s)	118, 151, 244, 1393
BfaI	CTAG	3 cut(s)	639, 681, 1904
BfmI	CTRYAG	2 cut(s)	1729, 2044
BfoI	RGCGCY	2 cut(s)	319, 2373
BfuAI	ACCTGC	2 cut(s)	100, 569
BfuI	GTATCC	1 cut(s)	22
BisI	GCNGC	8 cut(s)	496, 627, 1410, 1461, 1469, 1826, 1842, 2516
BlpI	GCTNAGC	1 cut(s)	1921
BlsI	GCNGC	8 cut(s)	497, 628, 1411, 1462, 1470, 1827, 1843, 2517
Bme1390I	CCNGG	5 cut(s)	16, 1006, 1234, 1963, 2109
BmgBI	CACGTC	1 cut(s)	145
BmgT120I	GGNCC	4 cut(s)	234, 594, 740, 1741
BmiI	GGNNCC	1 cut(s)	2435
BmrFI	CCNGG	5 cut(s)	16, 1006, 1234, 1963, 2109
BmrI	ACTGGG	3 cut(s)	241, 286, 795
BmsI	GCATC	7 cut(s)	526, 990, 1435, 1905, 2005, 2213, 2299
BmuI	ACTGGG	3 cut(s)	241, 286, 795
BplI	GAGNNNNNCTC	2 cut(s)	1694, 1726
BpmI	CTGGAG	3 cut(s)	2130, 2394, 2540
Bpu1102I	GCTNAGC	1 cut(s)	1921
Bpu14I	TTCGAA	1 cut(s)	507
BpuMI	CCSGG	2 cut(s)	16, 1006
BsaHI	GRCGYC	1 cut(s)	246
BsaI	GGTCTC	1 cut(s)	118
BsaJI	CCNNGG	4 cut(s)	117, 130, 1233, 1962
BsaWI	WCCGGW	1 cut(s)	1588
BsaXI	ACNNNNNCTCC	4 cut(s)	68, 98, 131, 161
Bsc4I	CCNNNNNNNGG	6 cut(s)	15, 77, 277, 280, 1882, 1963
Bse118I	RCCGGY	1 cut(s)	161
Bse1I	ACTGG	4 cut(s)	236, 281, 790, 2208
Bse3DI	GCAATG	1 cut(s)	1911
BseBI	CCWGG	3 cut(s)	1234, 1963, 2109
BseDI	CCNNGG	4 cut(s)	117, 130, 1233, 1962
BseLI	CCNNNNNNNGG	6 cut(s)	15, 77, 277, 280, 1882, 1963
BseMI	GCAATG	1 cut(s)	1911
BseMII	CTCAG	4 cut(s)	531, 806, 1836, 1935
BseNI	ACTGG	4 cut(s)	236, 281, 790, 2208
BseRI	GAGGAG	1 cut(s)	76
BseXI	GCAGC	7 cut(s)	638, 1421, 1455, 1472, 1812, 1828, 2502
BsgI	GTGCAG	1 cut(s)	645
Bsh1236I	CGCG	1 cut(s)	495
Bsh1285I	CGRYCG	2 cut(s)	256, 2324
BshNI	GGYRCC	1 cut(s)	2433
BsiEI	CGRYCG	2 cut(s)	256, 2324
BsiHKAI	GWGCWC	2 cut(s)	430, 1798
BsiSI	CCGG	5 cut(s)	16, 162, 1005, 1165, 1589
BslI	CCNNNNNNNGG	6 cut(s)	15, 77, 277, 280, 1882, 1963
BsmAI	GTCTC	4 cut(s)	118, 151, 244, 1393
BsmBI	CGTCTC	2 cut(s)	151, 244
BsmI	GAATGC	1 cut(s)	956
Bso31I	GGTCTC	1 cut(s)	118
Bsp119I	TTCGAA	1 cut(s)	507
Bsp1286I	GDGCHC	5 cut(s)	430, 793, 1798, 2100, 2457
Bsp1407I	TGTACA	2 cut(s)	1022, 1397
Bsp143I	GATC	8 cut(s)	453, 644, 663, 677, 745, 1211, 1313, 1719
Bsp1720I	GCTNAGC	1 cut(s)	1921
BspACI	CCGC	8 cut(s)	40, 63, 90, 256, 271, 495, 1337, 1425
BspCNI	CTCAG	4 cut(s)	532, 805, 1837, 1934
BspFNI	CGCG	1 cut(s)	495
BspLI	GGNNCC	1 cut(s)	2435
BspMI	ACCTGC	2 cut(s)	100, 569
BspPI	GGATC	2 cut(s)	685, 1321
BspT104I	TTCGAA	1 cut(s)	507
BspT107I	GGYRCC	1 cut(s)	2433
BspTNI	GGTCTC	1 cut(s)	118
BsrDI	GCAATG	1 cut(s)	1911
BsrFI	RCCGGY	1 cut(s)	161
BsrGI	TGTACA	2 cut(s)	1022, 1397
BsrI	ACTGG	4 cut(s)	236, 281, 790, 2208
BssAI	RCCGGY	1 cut(s)	161
BssECI	CCNNGG	4 cut(s)	117, 130, 1233, 1962
BssMI	GATC	8 cut(s)	453, 644, 663, 677, 745, 1211, 1313, 1719
BssNAI	GTATAC	1 cut(s)	1185
BssNI	GRCGYC	1 cut(s)	246
Bst1107I	GTATAC	1 cut(s)	1185
Bst2UI	CCWGG	3 cut(s)	1234, 1963, 2109
Bst4CI	ACNGT	6 cut(s)	10, 24, 383, 1609, 1646, 2325
Bst6I	CTCTTC	3 cut(s)	1250, 2482, 2496
BstACI	GRCGYC	1 cut(s)	246
BstAUI	TGTACA	2 cut(s)	1022, 1397
BstBI	TTCGAA	1 cut(s)	507
BstC8I	GCNNGC	8 cut(s)	430, 465, 493, 530, 624, 1830, 1918, 2453
BstDEI	CTNAG	4 cut(s)	540, 792, 1845, 1921
BstDSI	CCRYGG	2 cut(s)	117, 130
BstFNI	CGCG	1 cut(s)	495
BstH2I	RGCGCY	2 cut(s)	319, 2373
BstHHI	GCGC	3 cut(s)	318, 1121, 2372
BstKTI	GATC	8 cut(s)	456, 647, 666, 680, 748, 1214, 1316, 1722
BstMAI	GTCTC	4 cut(s)	118, 151, 244, 1393
BstMBI	GATC	8 cut(s)	453, 644, 663, 677, 745, 1211, 1313, 1719
BstMCI	CGRYCG	2 cut(s)	256, 2324
BstMWI	GCNNNNNNNGC	7 cut(s)	469, 1740, 1793, 1838, 1922, 2384, 2457
BstNI	CCWGG	3 cut(s)	1234, 1963, 2109
BstNSI	RCATGY	4 cut(s)	442, 619, 1832, 1920
BstSCI	CCNGG	5 cut(s)	14, 1004, 1232, 1961, 2107
BstSFI	CTRYAG	2 cut(s)	1729, 2044
BstUI	CGCG	1 cut(s)	495
BstV1I	GCAGC	7 cut(s)	638, 1421, 1455, 1472, 1812, 1828, 2502
BstX2I	RGATCY	2 cut(s)	677, 1313
BstYI	RGATCY	2 cut(s)	677, 1313
BstZ17I	GTATAC	1 cut(s)	1185
BsuI	GTATCC	1 cut(s)	22
BtgI	CCRYGG	2 cut(s)	117, 130
BtrI	CACGTC	1 cut(s)	145
BtsIMutI	CAGTG	8 cut(s)	274, 379, 432, 1295, 1605, 1651, 2017, 2215
BveI	ACCTGC	2 cut(s)	100, 569
Cac8I	GCNNGC	8 cut(s)	430, 465, 493, 530, 624, 1830, 1918, 2453
CfoI	GCGC	3 cut(s)	318, 1121, 2372
Cfr10I	RCCGGY	1 cut(s)	161
Cfr13I	GGNCC	4 cut(s)	234, 594, 740, 1741
Csp6I	GTAC	8 cut(s)	1023, 1038, 1398, 2291, 2338, 2434, 2538, 2557
CspCI	CAANNNNNGTGG	2 cut(s)	655, 690
CviQI	GTAC	8 cut(s)	1023, 1038, 1398, 2291, 2338, 2434, 2538, 2557
DdeI	CTNAG	4 cut(s)	540, 792, 1845, 1921
DpnI	GATC	8 cut(s)	455, 646, 665, 679, 747, 1213, 1315, 1721
DpnII	GATC	8 cut(s)	453, 644, 663, 677, 745, 1211, 1313, 1719
DraI	TTTAAA	1 cut(s)	2418
DraIII	CACNNNGTG	1 cut(s)	385
DrdI	GACNNNNNNGTC	2 cut(s)	236, 1425
DseDI	GACNNNNNNGTC	2 cut(s)	236, 1425
EaeI	YGGCCR	3 cut(s)	3, 17, 1166
Eam1104I	CTCTTC	3 cut(s)	1250, 2482, 2496
EarI	CTCTTC	3 cut(s)	1250, 2482, 2496
EciI	GGCGGA	2 cut(s)	79, 260
Eco147I	AGGCCT	1 cut(s)	1056
Eco24I	GRGCYC	3 cut(s)	793, 2100, 2457
Eco31I	GGTCTC	1 cut(s)	118
Eco32I	GATATC	1 cut(s)	2218
Eco47III	AGCGCT	1 cut(s)	2371
Eco57I	CTGAAG	1 cut(s)	1353
EcoRI	GAATTC	2 cut(s)	1371, 2316
EcoRII	CCWGG	3 cut(s)	1232, 1961, 2107
EcoRV	GATATC	1 cut(s)	2218
EcoT38I	GRGCYC	3 cut(s)	793, 2100, 2457
Esp3I	CGTCTC	2 cut(s)	151, 244
FauI	CCCGC	1 cut(s)	47
FauNDI	CATATG	2 cut(s)	1099, 2542
FbaI	TGATCA	2 cut(s)	453, 663
FblI	GTMKAC	3 cut(s)	252, 1184, 1707
Fnu4HI	GCNGC	8 cut(s)	496, 627, 1410, 1461, 1469, 1826, 1842, 2516
FriOI	GRGCYC	3 cut(s)	793, 2100, 2457
Fsp4HI	GCNGC	8 cut(s)	496, 627, 1410, 1461, 1469, 1826, 1842, 2516
FspBI	CTAG	3 cut(s)	639, 681, 1904
GlaI	GCGC	3 cut(s)	317, 1120, 2371
GluI	GCNGC	8 cut(s)	496, 627, 1410, 1461, 1469, 1826, 1842, 2516
GsuI	CTGGAG	3 cut(s)	2130, 2394, 2540
HaeII	RGCGCY	2 cut(s)	319, 2373
HapII	CCGG	5 cut(s)	16, 162, 1005, 1165, 1589
HhaI	GCGC	3 cut(s)	318, 1121, 2372
Hin1I	GRCGYC	1 cut(s)	246
Hin6I	GCGC	3 cut(s)	316, 1119, 2370
HinP1I	GCGC	3 cut(s)	316, 1119, 2370
HincII	GTYRAC	2 cut(s)	253, 2526
HindII	GTYRAC	2 cut(s)	253, 2526
HindIII	AAGCTT	2 cut(s)	699, 890
HpaII	CCGG	5 cut(s)	16, 162, 1005, 1165, 1589
HphI	GGTGA	6 cut(s)	131, 151, 160, 967, 1447, 2225
Hpy166II	GTNNAC	8 cut(s)	121, 200, 253, 1185, 1400, 1418, 1708, 2526
Hpy188I	TCNGA	4 cut(s)	244, 458, 1431, 2267
Hpy188III	TCNNGA	4 cut(s)	223, 848, 959, 1799
Hpy8I	GTNNAC	8 cut(s)	121, 200, 253, 1185, 1400, 1418, 1708, 2526
Hpy99I	CGWCG	3 cut(s)	248, 251, 254
HpyAV	CCTTC	6 cut(s)	103, 220, 823, 1046, 1426, 2491
HpyCH4III	ACNGT	6 cut(s)	10, 24, 383, 1609, 1646, 2325
HpyCH4IV	ACGT	2 cut(s)	144, 246
HpyF10VI	GCNNNNNNNGC	7 cut(s)	469, 1740, 1793, 1838, 1922, 2384, 2457
HpyF3I	CTNAG	4 cut(s)	540, 792, 1845, 1921
HpySE526I	ACGT	2 cut(s)	144, 246
Hsp92I	GRCGYC	1 cut(s)	246
HspAI	GCGC	3 cut(s)	316, 1119, 2370
KpnI	GGTACC	1 cut(s)	2437
Ksp22I	TGATCA	2 cut(s)	453, 663
Kzo9I	GATC	8 cut(s)	453, 644, 663, 677, 745, 1211, 1313, 1719
LmnI	GCTCC	4 cut(s)	273, 1360, 1801, 2375
Lsp1109I	GCAGC	7 cut(s)	638, 1421, 1455, 1472, 1812, 1828, 2502
LweI	GCATC	7 cut(s)	526, 990, 1435, 1905, 2005, 2213, 2299
MaeI	CTAG	3 cut(s)	639, 681, 1904
MaeII	ACGT	2 cut(s)	144, 246
MaeIII	GTNAC	4 cut(s)	751, 967, 1597, 1807
MalI	GATC	8 cut(s)	455, 646, 665, 679, 747, 1213, 1315, 1721
MboI	GATC	8 cut(s)	453, 644, 663, 677, 745, 1211, 1313, 1719
MfeI	CAATTG	2 cut(s)	1491, 2118
MflI	RGATCY	2 cut(s)	677, 1313
MhlI	GDGCHC	5 cut(s)	430, 793, 1798, 2100, 2457
MlsI	TGGCCA	1 cut(s)	5
MluNI	TGGCCA	1 cut(s)	5
MlyI	GAGTC	5 cut(s)	77, 213, 551, 748, 2531
MmeI	TCCRAC	3 cut(s)	267, 1924, 2245
Mox20I	TGGCCA	1 cut(s)	5
MroXI	GAANNNNTTC	1 cut(s)	2186
MscI	TGGCCA	1 cut(s)	5
MseI	TTAA	9 cut(s)	864, 1140, 1302, 1500, 1584, 1928, 1941, 2085, 2417
MslI	CAYNNNNRTG	1 cut(s)	437
Msp20I	TGGCCA	1 cut(s)	5
MspA1I	CMGCKG	1 cut(s)	1028
MspI	CCGG	5 cut(s)	16, 162, 1005, 1165, 1589
MspR9I	CCNGG	5 cut(s)	16, 1006, 1234, 1963, 2109
MunI	CAATTG	2 cut(s)	1491, 2118
Mva1269I	GAATGC	1 cut(s)	956
MvaI	CCWGG	3 cut(s)	1234, 1963, 2109
MvnI	CGCG	1 cut(s)	495
MwoI	GCNNNNNNNGC	7 cut(s)	469, 1740, 1793, 1838, 1922, 2384, 2457
NciI	CCSGG	2 cut(s)	16, 1006
NdeI	CATATG	2 cut(s)	1099, 2542
NdeII	GATC	8 cut(s)	453, 644, 663, 677, 745, 1211, 1313, 1719
NlaIV	GGNNCC	1 cut(s)	2435
NmeAIII	GCCGAG	1 cut(s)	448
NmuCI	GTSAC	2 cut(s)	751, 967
NspI	RCATGY	4 cut(s)	442, 619, 1832, 1920
NspV	TTCGAA	1 cut(s)	507
PaeI	GCATGC	2 cut(s)	1832, 1920
PceI	AGGCCT	1 cut(s)	1056
PciI	ACATGT	2 cut(s)	438, 615
PctI	GAATGC	1 cut(s)	956
PdmI	GAANNNNTTC	1 cut(s)	2186
PfeI	GAWTC	6 cut(s)	156, 601, 1592, 1760, 2131, 2251
PflFI	GACNNNGTC	1 cut(s)	248
PflMI	CCANNNNNTGG	1 cut(s)	1882
PkrI	GCNGC	8 cut(s)	497, 628, 1411, 1462, 1470, 1827, 1843, 2517
PleI	GAGTC	5 cut(s)	77, 212, 550, 748, 2530
PpsI	GAGTC	5 cut(s)	77, 212, 550, 748, 2530
PscI	ACATGT	2 cut(s)	438, 615
PshBI	ATTAAT	1 cut(s)	1302
PsiI	TTATAA	1 cut(s)	2396
Psp6I	CCWGG	3 cut(s)	1232, 1961, 2107
PspGI	CCWGG	3 cut(s)	1232, 1961, 2107
PspN4I	GGNNCC	1 cut(s)	2435
PspPI	GGNCC	4 cut(s)	234, 594, 740, 1741
PsuI	RGATCY	2 cut(s)	677, 1313
PsyI	GACNNNGTC	1 cut(s)	248
PvuII	CAGCTG	1 cut(s)	1028
RsaI	GTAC	8 cut(s)	1024, 1039, 1399, 2292, 2339, 2435, 2539, 2558
RsaNI	GTAC	8 cut(s)	1023, 1038, 1398, 2291, 2338, 2434, 2538, 2557
RseI	CAYNNNNRTG	1 cut(s)	437
SalI	GTCGAC	1 cut(s)	251
SaqAI	TTAA	9 cut(s)	864, 1140, 1302, 1500, 1584, 1928, 1941, 2085, 2417
SatI	GCNGC	8 cut(s)	496, 627, 1410, 1461, 1469, 1826, 1842, 2516
Sau3AI	GATC	8 cut(s)	453, 644, 663, 677, 745, 1211, 1313, 1719
Sau96I	GGNCC	4 cut(s)	234, 594, 740, 1741
SchI	GAGTC	5 cut(s)	77, 213, 551, 748, 2531
ScrFI	CCNGG	5 cut(s)	16, 1006, 1234, 1963, 2109
SduI	GDGCHC	5 cut(s)	430, 793, 1798, 2100, 2457
SfaNI	GCATC	7 cut(s)	526, 990, 1435, 1905, 2005, 2213, 2299
SfcI	CTRYAG	2 cut(s)	1729, 2044
SfuI	TTCGAA	1 cut(s)	507
SmiMI	CAYNNNNRTG	1 cut(s)	437
SphI	GCATGC	2 cut(s)	1832, 1920
SseBI	AGGCCT	1 cut(s)	1056
SsiI	CCGC	8 cut(s)	40, 63, 90, 256, 271, 495, 1337, 1425
SspI	AATATT	3 cut(s)	763, 868, 2282
SspMI	CTAG	3 cut(s)	639, 681, 1904
StuI	AGGCCT	1 cut(s)	1056
StyD4I	CCNGG	5 cut(s)	14, 1004, 1232, 1961, 2107
TaaI	ACNGT	6 cut(s)	10, 24, 383, 1609, 1646, 2325
TaiI	ACGT	2 cut(s)	147, 249
TaqI	TCGA	7 cut(s)	207, 252, 288, 483, 507, 574, 2320
TatI	WGTACW	3 cut(s)	1022, 1037, 1397
TauI	GCSGC	1 cut(s)	498
TfiI	GAWTC	6 cut(s)	156, 601, 1592, 1760, 2131, 2251
Tru1I	TTAA	9 cut(s)	864, 1140, 1302, 1500, 1584, 1928, 1941, 2085, 2417
Tru9I	TTAA	9 cut(s)	864, 1140, 1302, 1500, 1584, 1928, 1941, 2085, 2417
TscAI	CASTG	8 cut(s)	281, 386, 439, 1302, 1612, 1651, 2017, 2215
TseFI	GTSAC	2 cut(s)	751, 967
TseI	GCWGC	7 cut(s)	626, 1409, 1460, 1468, 1825, 1841, 2515
Tsp45I	GTSAC	2 cut(s)	751, 967
TspGWI	ACGGA	3 cut(s)	106, 833, 1221
TspRI	CASTG	8 cut(s)	281, 386, 439, 1302, 1612, 1651, 2017, 2215
Tth111I	GACNNNGTC	1 cut(s)	248
Van91I	CCANNNNNTGG	1 cut(s)	1882
VspI	ATTAAT	1 cut(s)	1302
XapI	RAATTY	5 cut(s)	1371, 1525, 1686, 2001, 2316
XceI	RCATGY	4 cut(s)	442, 619, 1832, 1920
XcmI	CCANNNNNNNNNTGG	1 cut(s)	604
XmiI	GTMKAC	3 cut(s)	252, 1184, 1707
XmnI	GAANNNNTTC	1 cut(s)	2186
XspI	CTAG	3 cut(s)	639, 681, 1904
ZraI	GACGTC	1 cut(s)	247

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.