Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr3

Orthogroup ID

OG0001672

Physical Location & Seq

Genomic Coordinates Forward (+)

27579082 .. 27586066

Gene Structure 6985 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw3G022490.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2583 bp

ATGGCCACTCTAGTATCCCAGCCACCCCTCCTTCACCTTCCGCAGTCCAAGACCCGAATTCTCTGCAACAAGCCCAACAACAACAACAAGAAGAGGACCGACTCAGCAGCATTCAAGGAGAAGAAGCACGTCTCCGTCGACTACGACAGCGGCACTCACCACCTCTCCACTCATATCGGCGGCTTCAGGAAGCGAGACATCCCCAAACACCACCGCTTACGCGTCGAAACGGAGCGTTTCCAGAAGGACTGGGCCGTCTCCGACGTCGTCAATCGGGTCTATCAGCTCCGTCGCGGTGACGACATTGACGGCGTGCTCAATCGGTGGGTCGGACGCTTCGCCAGGAAGAACTTCCCCATCCTAATTAGAGAAATGACGGCGAGGGGGTCGGTGGAGCACTGTATGCAAGTGTTCCGGTGGATGAAGACCCTCAAGAACTACTGTGCTCGCACTGATATTTATAACATGTTGATCAGATTGCACGCCAGGCATAGTCTTGTTGATCAAGCTCGCGGCTTGTTTTTCGAAATGCAAGAATGGAGGTGCAAGCCTGATGCTGAGTCCTACAATGCTCTTATCAATGCACATGGTCGAGCTGGTCAATGGCGCTGGGCCATGAACATTATGGATGACATGCTGCGTGCAGCTATCCCTCCTAGTCGGTCAACATATAACAACTTGATCAATGCATGTGGATCTAGTGGAAATTGGAGAGAAGCTTTAAAAGTTTGCAAGAAAATGACAGATAATGGAGTTGGACCTGATCTTGTGACTCACAATATTGTTTTATCTGCATACAAAACTGGGGCTCAATATTCAAAAGCTTTATCTTATTTTGAACTTATGAAGGGAACAAATATCCGTCCAGACACAACGACCCTCAATATTGTTATATATTGCCTAGTGAAGCTTGGACAGTATGGGAAAGCCATTGATATTTTCAATTCCATGAGAGATAAGGGAGCAGAATGTCGCCCTGACATCGTAACATTCACCAGCATCATTCATTTGTATTCTGTGAGCGGGCAGATAGACAACTGCACAGCCGTATTTAATACAATTCTTGCAGAAGGGCTAAAACCTAACATTGTTTCCTATAATGCACTATTAGGTGCATATGCTTCACATGGGATGTGCGCAGAGGCGGCATCAGTATTTAATGAGATGAAGAAAAGTAGTTTTCGGCCAGATGTTGTGTCTTATACATCTCTACTCAATGCTTATGGAAGATCACGACATCCTAAGAAGGCTAGGGAAGTTTTTGACAGGATGAAGGCCAACCACTTGAAGCCAAACCTTGTGAGCTACAACGCACTGATTAATGCTTATGGATCCAGTGGTTTATTAGCCGAGGCTGTTGAGGTCTTGCGTGAGATGGAGCAAAATGGAATTCATCCAAACATTGTCTCAATATGTACCCTTTTGGCTGCTTGTGGACAATGTGGCCAGAAGGTGAAAATTGATGCCATACTTTCAGCAGCCAAGCTACGAGGCATTGAACTGAACACAATTGCCTATAATTCGGCTATTGGGAGCTATATGAATTTGGGAGAACATGAGAAAGCTATAAACTTGTATCAATCTATGAGAAAGAAGAAAGTGAAACCGGATTCTGTTACTTACAATGTGTTGATAAGTGGTTGTTGTAAGATGTCAAAATACAGTGAGGCACTAACCTTTTTTGATGAAATGGTGGATTTGAAAATTCCTATGTCCAAAGAAGTCTACTCATCTGTGCTCTGTGCCTACAACAAGAAGGGCCAACTTACGGAAGCGGAATCTGTATTCAACTCAATGAAGATGGCTGGATGTCCTCCTGATGTAGTTACATATACTGCAATGTTACATGCATATAGTGCTGCAGAGAATTGGGAAAAGGCTTGTGAGCTATTTCAAGAAATGGAAATAAATGGCATCCAACCAGATGCTATCGCATGTTCAGCTCTGATGAGAGTTTTTAACAAAGGAGGCCATCCCTCTAGAGTTCTTTTTCTGGCAGAACTCATGAGAGAAAAAGAAATTCCTTTCAATGATGCCATTTTCTTTGAAATGGTTTCGGCTTGTAGCTTATTACAAGATTGGAGAACAACAATACAATTGATTAAGGTAATAGAGCCCTCATTCCCTAGACTTTCAGTCGGGCTTAAGAATCAGCTCCTGCAGATTATGGGAAGAAGCGGAAAGACAGAGGCTATGATGAAGTTGTTCTACAAGATAATTGCATCCAATGGTGATATCAACTTTGATACTTACTCAATTTTGTTGAAGAATCTTTTGTCTGTTGGAAGTTGGAGGAAATATATTGAGGTATTGCAGTGGATGGTGGATGCTAGAGTACGACCTTCAGGCCAAATGTATCTCGATATATCCTGTTTTGCACGGAAAAGTGGTGGAACTGAATATGCTTCTATTATAAATGAAAGAATCGAATTATTGAAAAGAAAAGCAAGGGAAGAAGACTCTCGAAGCAAGCTACGTGATACTCACTCCACTTCCATGCTGGTACATAGAACAAGAGCAGAGAACCACAGGCTGCTTGAATCAAGAATGGCTGTTACGCATCCTGTAGTTCCTGACTGCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003676 GO:0003723 GO:0003735 GO:0003824 GO:0004518 GO:0004519 GO:0005198 GO:0005488 GO:0006139 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0008380 GO:0009451 GO:0009790 GO:0009791 GO:0009793 GO:0009987 GO:0010154 GO:0010239 GO:0010467 GO:0016070 GO:0016071 GO:0016787 GO:0016788 GO:0022414 GO:0031425 GO:0032501 GO:0032502 GO:0034641 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0046483 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0061458 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_2 PPR_3

Protein Analysis

860

Amino Acids

97.27

Weight (kDa)

9.3

Isoelectric Point (pI)

37.63

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	153 - 193	4.4e-08	PPR repeat family
PPR_3	PF13812	174 - 232	6e-08	Pentatricopeptide repeat domain
PPR_2	PF13041	184 - 231	5.7e-11	PPR repeat family
TPR_24	PF23276	197 - 317	9.4e-10	Fungal tetratrico peptide repeats
PPR_long	PF17177	205 - 319	3.9e-08	Pentacotripeptide-repeat region of PRORP
PPR_3	PF13812	208 - 266	5.4e-09	Pentatricopeptide repeat domain
PPR_2	PF13041	219 - 266	7e-11	PPR repeat family
PPR	PF01535	222 - 252	1.7e-07	PPR repeat
PPR_long	PF17177	310 - 406	4.8e-07	Pentacotripeptide-repeat region of PRORP
PPR_3	PF13812	313 - 373	2.6e-10	Pentatricopeptide repeat domain
PPR_2	PF13041	326 - 366	7.8e-06	PPR repeat family
PPR_1	PF12854	358 - 390	4.6e-06	PPR repeat
PPR_2	PF13041	361 - 408	5.3e-15	PPR repeat family
PPR	PF01535	364 - 393	3.6e-07	PPR repeat
PPR_long	PF17177	380 - 492	2.9e-10	Pentacotripeptide-repeat region of PRORP
PPR_3	PF13812	384 - 444	2.1e-18	Pentatricopeptide repeat domain
PPR_2	PF13041	396 - 442	9.6e-13	PPR repeat family
PPR	PF01535	399 - 427	1.2e-06	PPR repeat
TPR_24	PF23276	403 - 529	2.1e-11	Fungal tetratrico peptide repeats
PPR_1	PF12854	428 - 460	1.2e-09	PPR repeat
PPR_2	PF13041	431 - 478	9.6e-12	PPR repeat family
PPR	PF01535	434 - 464	1.7e-08	PPR repeat
PPR_3	PF13812	490 - 550	2.3e-10	Pentatricopeptide repeat domain
PPR_2	PF13041	502 - 550	4.2e-17	PPR repeat family
PPR_long	PF17177	520 - 663	3.3e-15	Pentacotripeptide-repeat region of PRORP
PPR_1	PF12854	533 - 565	2.8e-10	PPR repeat
PPR_2	PF13041	537 - 580	7.5e-09	PPR repeat family
PPR	PF01535	539 - 567	2.3e-08	PPR repeat
PPR_3	PF13812	561 - 618	6.7e-07	Pentatricopeptide repeat domain
PPR_2	PF13041	575 - 618	1.4e-11	PPR repeat family
PPR_1	PF12854	603 - 635	9.5e-08	PPR repeat
PPR_3	PF13812	606 - 653	7.5e-08	Pentatricopeptide repeat domain
PPR_2	PF13041	606 - 653	2.1e-12	PPR repeat family
PPR	PF01535	609 - 639	3.7e-07	PPR repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0001672)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G41720 AT2G41720 AT2G41720
fragaria_vesca	FvH4_6g24060 FvH4_6g24060 FvH4_6g24060 FvH4_6g24060 FvH4_6g24060 FvH4_6g24060 FvH4_6g24060 FvH4_6g24060 FvH4_6g24060 FvH4_6g24060
malus_domestica	MD12G1080100.v1.1
prunus_persica	Prupe.7G058400_v2.0.a1 Prupe.7G058400_v2.0.a1 Prupe.7G058400_v2.0.a1
rosa_chinensis	RchiOBHm_Chr3g0482651
rosa_laevigata	RLG00000001090 RLG00000016982 RLG00000023335 RLG00000028152 RLG00000032501
rosa_multiflora	Rmu_sc0004946.1_g000004 Rmu_sc0006069.1_g000004 Rmu_sc0015893.1_g000010
rosa_roxburghii	Rroxscaffold_3G00229260 Rroxscaffold_3G00244090 Rroxscaffold_4G00320230 Rroxscaffold_6G00399060 Rroxscaffold_7G00168600
rosa_rugosa	Rorug03G0197600 Rorug03G0197700
rosa_samantha	Rh3AG051200 Rh3AG249000 Rh3BG052500 Rh3BG284300 Rh3CG052100 Rh3CG282200 Rh3DG052800 Rh3DG278200 Rh4BG176200 Rh6AG132300 Rh7AG450400 Rh7BG246700 Rh7BG421800 Rh7BG421900 Rh7CG470100
rosa_wichuraiana	Rw3G022490

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	462, 2414
AasI	GACNNNNNNGTC	1 cut(s)	254
AatII	GACGTC	1 cut(s)	267
Acc16I	TGCGCA	1 cut(s)	1138
AccBSI	CCGCTC	1 cut(s)	1023
AccI	GTMKAC	2 cut(s)	138, 1725
AccII	CGCG	3 cut(s)	222, 294, 513
AclWI	GGATC	3 cut(s)	703, 1326, 1339
AcoI	YGGCCR	3 cut(s)	3, 1184, 1444
AcsI	RAATTY	5 cut(s)	57, 1389, 1543, 1704, 2019
AcuI	CTGAAG	2 cut(s)	169, 2328
AcyI	GRCGYC	1 cut(s)	264
AfaI	GTAC	3 cut(s)	1417, 2337, 2505
AfiI	CCNNNNNNNGG	1 cut(s)	1768
AflII	CTTAAG	1 cut(s)	2144
AflIII	ACRYGT	2 cut(s)	220, 465
AhdI	GACNNNNNGTC	1 cut(s)	2135
AjiI	CACGTC	1 cut(s)	130
AjnI	CCWGG	2 cut(s)	341, 485
Alw21I	GWGCWC	4 cut(s)	318, 399, 448, 1740
Alw26I	GTCTC	4 cut(s)	136, 189, 262, 1411
AlwI	GGATC	3 cut(s)	703, 1326, 1339
AlwNI	CAGNNNCTG	1 cut(s)	2158
AoxI	GGCC	9 cut(s)	3, 252, 612, 1184, 1275, 1444, 1759, 1969, 2347
ApeKI	GCWGC	7 cut(s)	107, 637, 644, 1427, 1478, 1859, 2533
ApoI	RAATTY	5 cut(s)	57, 1389, 1543, 1704, 2019
AseI	ATTAAT	1 cut(s)	1320
Asp700I	GAANNNNTTC	2 cut(s)	350, 2204
AspLEI	GCGC	2 cut(s)	609, 1139
AspS9I	GGNCC	5 cut(s)	96, 252, 612, 758, 1759
AsuHPI	GGTGA	6 cut(s)	26, 149, 308, 985, 1465, 2243
AsuII	TTCGAA	1 cut(s)	525
AvaII	GGWCC	2 cut(s)	96, 758
BaeI	ACNNNNGTAYC	1 cut(s)	30
BalI	TGGCCA	2 cut(s)	5, 1446
BamHI	GGATCC	1 cut(s)	1331
BanII	GRGCYC	2 cut(s)	811, 2118
BarI	GAAGNNNNNNTAC	2 cut(s)	2192, 2224
BbsI	GAAGAC	2 cut(s)	431, 2463
Bbv12I	GWGCWC	4 cut(s)	318, 399, 448, 1740
BbvI	GCAGC	7 cut(s)	119, 624, 656, 1414, 1490, 1846, 2520
BccI	CCATC	5 cut(s)	365, 1369, 1795, 1980, 2314
BceAI	ACGGC	4 cut(s)	239, 325, 393, 1031
BcgI	CGANNNNNNTGC	2 cut(s)	45, 79
BciT130I	CCWGG	2 cut(s)	343, 487
BciVI	GTATCC	1 cut(s)	25
BclI	TGATCA	3 cut(s)	471, 502, 681
BcoDI	GTCTC	4 cut(s)	136, 189, 262, 1411
BfaI	CTAG	8 cut(s)	11, 657, 699, 902, 1251, 1980, 2127, 2331
BfmI	CTRYAG	3 cut(s)	1860, 2159, 2565
BfoI	RGCGCY	1 cut(s)	610
BfrI	CTTAAG	1 cut(s)	2144
BfuI	GTATCC	1 cut(s)	25
Bme1390I	CCNGG	2 cut(s)	343, 487
Bme18I	GGWCC	2 cut(s)	96, 758
BmeRI	GACNNNNNGTC	1 cut(s)	2135
BmgBI	CACGTC	1 cut(s)	130
BmgT120I	GGNCC	5 cut(s)	96, 252, 612, 758, 1759
BmiI	GGNNCC	1 cut(s)	1333
BmrFI	CCNGG	2 cut(s)	343, 487
BmrI	ACTGGG	2 cut(s)	259, 813
BmuI	ACTGGG	2 cut(s)	259, 813
BpiI	GAAGAC	2 cut(s)	431, 2463
Bpu14I	TTCGAA	1 cut(s)	525
BpuEI	CTTGAG	1 cut(s)	416
BsaAI	YACGTR	1 cut(s)	2477
BsaHI	GRCGYC	1 cut(s)	264
BsaJI	CCNNGG	1 cut(s)	1350
BsaWI	WCCGGW	2 cut(s)	414, 1606
BsaXI	ACNNNNNCTCC	2 cut(s)	149, 179
Bsc4I	CCNNNNNNNGG	1 cut(s)	1768
Bse1I	ACTGG	3 cut(s)	254, 808, 1335
Bse3DI	GCAATG	1 cut(s)	1845
BseBI	CCWGG	2 cut(s)	343, 487
BseDI	CCNNGG	1 cut(s)	1350
BseLI	CCNNNNNNNGG	1 cut(s)	1768
BseMI	GCAATG	1 cut(s)	1845
BseMII	CTCAG	2 cut(s)	117, 549
BseNI	ACTGG	3 cut(s)	254, 808, 1335
BseXI	GCAGC	7 cut(s)	119, 624, 656, 1414, 1490, 1846, 2520
BseYI	CCCAGC	2 cut(s)	18, 609
BsgI	GTGCAG	2 cut(s)	663, 1024
Bsh1236I	CGCG	3 cut(s)	222, 294, 513
BshFI	GGCC	9 cut(s)	5, 254, 614, 1186, 1277, 1446, 1761, 1971, 2349
BsiHKAI	GWGCWC	4 cut(s)	318, 399, 448, 1740
BsiSI	CCGG	2 cut(s)	415, 1607
BslI	CCNNNNNNNGG	1 cut(s)	1768
BsmAI	GTCTC	4 cut(s)	136, 189, 262, 1411
BsmBI	CGTCTC	2 cut(s)	136, 262
BsmI	GAATGC	1 cut(s)	110
BsnI	GGCC	9 cut(s)	5, 254, 614, 1186, 1277, 1446, 1761, 1971, 2349
Bsp119I	TTCGAA	1 cut(s)	525
Bsp1286I	GDGCHC	6 cut(s)	318, 399, 448, 811, 1740, 2118
Bsp143I	GATC	7 cut(s)	471, 502, 681, 695, 763, 1229, 1331
BspANI	GGCC	9 cut(s)	5, 254, 614, 1186, 1277, 1446, 1761, 1971, 2349
BspCNI	CTCAG	2 cut(s)	116, 550
BspFNI	CGCG	3 cut(s)	222, 294, 513
BspHI	TCATGA	1 cut(s)	2004
BspLI	GGNNCC	1 cut(s)	1333
BspMAI	CTGCAG	2 cut(s)	1864, 2163
BspPI	GGATC	3 cut(s)	703, 1326, 1339
BspT104I	TTCGAA	1 cut(s)	525
BspTI	CTTAAG	1 cut(s)	2144
BsrBI	CCGCTC	1 cut(s)	1023
BsrDI	GCAATG	1 cut(s)	1845
BsrI	ACTGG	3 cut(s)	254, 808, 1335
BssECI	CCNNGG	1 cut(s)	1350
BssMI	GATC	7 cut(s)	471, 502, 681, 695, 763, 1229, 1331
BssNI	GRCGYC	1 cut(s)	264
Bst2UI	CCWGG	2 cut(s)	343, 487
Bst4CI	ACNGT	4 cut(s)	401, 443, 918, 1664
Bst6I	CTCTTC	1 cut(s)	86
BstACI	GRCGYC	1 cut(s)	264
BstAFI	CTTAAG	1 cut(s)	2144
BstAPI	GCANNNNNTGC	2 cut(s)	403, 1856
BstBAI	YACGTR	1 cut(s)	2477
BstBI	TTCGAA	1 cut(s)	525
BstC8I	GCNNGC	8 cut(s)	314, 448, 483, 511, 548, 642, 1025, 2471
BstDEI	CTNAG	3 cut(s)	103, 558, 1242
BstFNI	CGCG	3 cut(s)	222, 294, 513
BstH2I	RGCGCY	1 cut(s)	610
BstHHI	GCGC	2 cut(s)	609, 1139
BstKTI	GATC	7 cut(s)	474, 505, 684, 698, 766, 1232, 1334
BstMAI	GTCTC	4 cut(s)	136, 189, 262, 1411
BstMBI	GATC	7 cut(s)	471, 502, 681, 695, 763, 1229, 1331
BstMWI	GCNNNNNNNGC	5 cut(s)	403, 487, 1145, 1856, 1940
BstNI	CCWGG	2 cut(s)	343, 487
BstNSI	RCATGY	5 cut(s)	469, 637, 693, 1850, 1938
BstSCI	CCNGG	2 cut(s)	341, 485
BstSFI	CTRYAG	3 cut(s)	1860, 2159, 2565
BstUI	CGCG	3 cut(s)	222, 294, 513
BstV1I	GCAGC	7 cut(s)	119, 624, 656, 1414, 1490, 1846, 2520
BstV2I	GAAGAC	2 cut(s)	431, 2463
BstX2I	RGATCY	2 cut(s)	695, 1331
BstYI	RGATCY	2 cut(s)	695, 1331
BsuI	GTATCC	1 cut(s)	25
BsuRI	GGCC	9 cut(s)	5, 254, 614, 1186, 1277, 1446, 1761, 1971, 2349
BtrI	CACGTC	1 cut(s)	130
BtsI	GCAGTG	1 cut(s)	2321
BtsIMutI	CAGTG	6 cut(s)	397, 450, 1313, 1342, 1669, 2321
Cac8I	GCNNGC	8 cut(s)	314, 448, 483, 511, 548, 642, 1025, 2471
CaiI	CAGNNNCTG	1 cut(s)	2158
CciI	TCATGA	1 cut(s)	2004
CfoI	GCGC	2 cut(s)	609, 1139
Cfr13I	GGNCC	5 cut(s)	96, 252, 612, 758, 1759
CseI	GACGC	2 cut(s)	211, 342
Csp6I	GTAC	3 cut(s)	1416, 2336, 2504
CspCI	CAANNNNNGTGG	2 cut(s)	673, 708
CviQI	GTAC	3 cut(s)	1416, 2336, 2504
DdeI	CTNAG	3 cut(s)	103, 558, 1242
DpnI	GATC	7 cut(s)	473, 504, 683, 697, 765, 1231, 1333
DpnII	GATC	7 cut(s)	471, 502, 681, 695, 763, 1229, 1331
DraI	TTTAAA	1 cut(s)	723
DrdI	GACNNNNNNGTC	1 cut(s)	254
DriI	GACNNNNNGTC	1 cut(s)	2135
DseDI	GACNNNNNNGTC	1 cut(s)	254
EaeI	YGGCCR	3 cut(s)	3, 1184, 1444
Eam1104I	CTCTTC	1 cut(s)	86
Eam1105I	GACNNNNNGTC	1 cut(s)	2135
EarI	CTCTTC	1 cut(s)	86
Eco24I	GRGCYC	2 cut(s)	811, 2118
Eco32I	GATATC	1 cut(s)	2236
Eco47I	GGWCC	2 cut(s)	96, 758
Eco57I	CTGAAG	2 cut(s)	169, 2328
EcoRI	GAATTC	2 cut(s)	57, 1389
EcoRII	CCWGG	2 cut(s)	341, 485
EcoRV	GATATC	1 cut(s)	2236
EcoT22I	ATGCAT	2 cut(s)	691, 1852
EcoT38I	GRGCYC	2 cut(s)	811, 2118
Esp3I	CGTCTC	2 cut(s)	136, 262
FauI	CCCGC	1 cut(s)	1016
FauNDI	CATATG	1 cut(s)	1117
FbaI	TGATCA	3 cut(s)	471, 502, 681
FblI	GTMKAC	2 cut(s)	138, 1725
FriOI	GRGCYC	2 cut(s)	811, 2118
FspBI	CTAG	8 cut(s)	11, 657, 699, 902, 1251, 1980, 2127, 2331
FspI	TGCGCA	1 cut(s)	1138
GlaI	GCGC	2 cut(s)	608, 1138
GsaI	CCCAGC	2 cut(s)	22, 613
HaeII	RGCGCY	1 cut(s)	610
HaeIII	GGCC	9 cut(s)	5, 254, 614, 1186, 1277, 1446, 1761, 1971, 2349
HapII	CCGG	2 cut(s)	415, 1607
HgaI	GACGC	2 cut(s)	211, 342
HhaI	GCGC	2 cut(s)	609, 1139
Hin1I	GRCGYC	1 cut(s)	264
Hin6I	GCGC	2 cut(s)	607, 1137
HinP1I	GCGC	2 cut(s)	607, 1137
HincII	GTYRAC	2 cut(s)	139, 666
HindII	GTYRAC	2 cut(s)	139, 666
HindIII	AAGCTT	3 cut(s)	717, 822, 908
HpaII	CCGG	2 cut(s)	415, 1607
HphI	GGTGA	6 cut(s)	26, 149, 308, 985, 1465, 2243
Hpy166II	GTNNAC	4 cut(s)	139, 666, 1436, 1726
Hpy188I	TCNGA	4 cut(s)	262, 332, 476, 1947
Hpy8I	GTNNAC	4 cut(s)	139, 666, 1436, 1726
Hpy99I	CGWCG	5 cut(s)	140, 227, 266, 269, 294
HpyCH4III	ACNGT	4 cut(s)	401, 443, 918, 1664
HpyCH4IV	ACGT	3 cut(s)	129, 264, 2476
HpyF10VI	GCNNNNNNNGC	5 cut(s)	403, 487, 1145, 1856, 1940
HpyF3I	CTNAG	3 cut(s)	103, 558, 1242
HpySE526I	ACGT	3 cut(s)	129, 264, 2476
Hsp92I	GRCGYC	1 cut(s)	264
HspAI	GCGC	2 cut(s)	607, 1137
Ksp22I	TGATCA	3 cut(s)	471, 502, 681
Kzo9I	GATC	7 cut(s)	471, 502, 681, 695, 763, 1229, 1331
LmnI	GCTCC	7 cut(s)	232, 291, 394, 962, 1378, 1533, 2160
Lsp1109I	GCAGC	7 cut(s)	119, 624, 656, 1414, 1490, 1846, 2520
MaeI	CTAG	8 cut(s)	11, 657, 699, 902, 1251, 1980, 2127, 2331
MaeII	ACGT	3 cut(s)	129, 264, 2476
MaeIII	GTNAC	7 cut(s)	296, 769, 985, 1615, 1825, 1842, 2554
MalI	GATC	7 cut(s)	473, 504, 683, 697, 765, 1231, 1333
MbiI	CCGCTC	1 cut(s)	1023
MboI	GATC	7 cut(s)	471, 502, 681, 695, 763, 1229, 1331
MfeI	CAATTG	2 cut(s)	1509, 2096
MflI	RGATCY	2 cut(s)	695, 1331
MhlI	GDGCHC	6 cut(s)	318, 399, 448, 811, 1740, 2118
MlsI	TGGCCA	2 cut(s)	5, 1446
MluI	ACGCGT	1 cut(s)	220
MluNI	TGGCCA	2 cut(s)	5, 1446
MlyI	GAGTC	4 cut(s)	95, 569, 766, 2453
MmeI	TCCRAC	6 cut(s)	285, 310, 736, 1942, 2263, 2270
Mox20I	TGGCCA	2 cut(s)	5, 1446
Mph1103I	ATGCAT	2 cut(s)	691, 1852
MroXI	GAANNNNTTC	2 cut(s)	350, 2204
MscI	TGGCCA	2 cut(s)	5, 1446
MseI	TTAA	7 cut(s)	722, 1053, 1158, 1320, 1959, 2103, 2145
MslI	CAYNNNNRTG	1 cut(s)	2495
Msp20I	TGGCCA	2 cut(s)	5, 1446
MspA1I	CMGCKG	1 cut(s)	150
MspCI	CTTAAG	1 cut(s)	2144
MspI	CCGG	2 cut(s)	415, 1607
MspR9I	CCNGG	2 cut(s)	343, 487
MunI	CAATTG	2 cut(s)	1509, 2096
Mva1269I	GAATGC	1 cut(s)	110
MvaI	CCWGG	2 cut(s)	343, 487
MvnI	CGCG	3 cut(s)	222, 294, 513
MwoI	GCNNNNNNNGC	5 cut(s)	403, 487, 1145, 1856, 1940
NdeI	CATATG	1 cut(s)	1117
NdeII	GATC	7 cut(s)	471, 502, 681, 695, 763, 1229, 1331
NlaIV	GGNNCC	1 cut(s)	1333
NmeAIII	GCCGAG	1 cut(s)	1375
NmuCI	GTSAC	2 cut(s)	296, 769
NsbI	TGCGCA	1 cut(s)	1138
NsiI	ATGCAT	2 cut(s)	691, 1852
NspI	RCATGY	5 cut(s)	469, 637, 693, 1850, 1938
NspV	TTCGAA	1 cut(s)	525
PagI	TCATGA	1 cut(s)	2004
PciI	ACATGT	1 cut(s)	465
PcsI	WCGNNNNNNNCGW	1 cut(s)	135
PctI	GAATGC	1 cut(s)	110
PdmI	GAANNNNTTC	2 cut(s)	350, 2204
PfeI	GAWTC	6 cut(s)	1610, 1778, 2149, 2269, 2424, 2540
PflFI	GACNNNGTC	1 cut(s)	266
PleI	GAGTC	4 cut(s)	95, 568, 766, 2453
PpsI	GAGTC	4 cut(s)	95, 568, 766, 2453
Ppu21I	YACGTR	1 cut(s)	2477
PscI	ACATGT	1 cut(s)	465
PshBI	ATTAAT	1 cut(s)	1320
PsiI	TTATAA	2 cut(s)	462, 2414
Psp6I	CCWGG	2 cut(s)	341, 485
PspFI	CCCAGC	2 cut(s)	18, 609
PspGI	CCWGG	2 cut(s)	341, 485
PspN4I	GGNNCC	1 cut(s)	1333
PspPI	GGNCC	5 cut(s)	96, 252, 612, 758, 1759
PstI	CTGCAG	2 cut(s)	1864, 2163
PstNI	CAGNNNCTG	1 cut(s)	2158
PsuI	RGATCY	2 cut(s)	695, 1331
PsyI	GACNNNGTC	1 cut(s)	266
RsaI	GTAC	3 cut(s)	1417, 2337, 2505
RsaNI	GTAC	3 cut(s)	1416, 2336, 2504
RseI	CAYNNNNRTG	1 cut(s)	2495
SalI	GTCGAC	1 cut(s)	137
SaqAI	TTAA	7 cut(s)	722, 1053, 1158, 1320, 1959, 2103, 2145
Sau3AI	GATC	7 cut(s)	471, 502, 681, 695, 763, 1229, 1331
Sau96I	GGNCC	5 cut(s)	96, 252, 612, 758, 1759
SchI	GAGTC	4 cut(s)	95, 569, 766, 2453
ScrFI	CCNGG	2 cut(s)	343, 487
SduI	GDGCHC	6 cut(s)	318, 399, 448, 811, 1740, 2118
SfcI	CTRYAG	3 cut(s)	1860, 2159, 2565
SfuI	TTCGAA	1 cut(s)	525
SinI	GGWCC	2 cut(s)	96, 758
SmiMI	CAYNNNNRTG	1 cut(s)	2495
SmlI	CTYRAG	2 cut(s)	431, 2144
SmoI	CTYRAG	2 cut(s)	431, 2144
SspI	AATATT	3 cut(s)	781, 815, 886
SspMI	CTAG	8 cut(s)	11, 657, 699, 902, 1251, 1980, 2127, 2331
StyD4I	CCNGG	2 cut(s)	341, 485
TaaI	ACNGT	4 cut(s)	401, 443, 918, 1664
TaiI	ACGT	3 cut(s)	132, 267, 2479
TaqI	TCGA	7 cut(s)	138, 225, 525, 592, 2361, 2427, 2464
TaqII	GACCGA	2 cut(s)	113, 651
TauI	GCSGC	4 cut(s)	153, 183, 516, 1148
TfiI	GAWTC	6 cut(s)	1610, 1778, 2149, 2269, 2424, 2540
Tru1I	TTAA	7 cut(s)	722, 1053, 1158, 1320, 1959, 2103, 2145
Tru9I	TTAA	7 cut(s)	722, 1053, 1158, 1320, 1959, 2103, 2145
TscAI	CASTG	6 cut(s)	404, 457, 1320, 1342, 1669, 2321
TseFI	GTSAC	2 cut(s)	296, 769
TseI	GCWGC	7 cut(s)	107, 637, 644, 1427, 1478, 1859, 2533
Tsp45I	GTSAC	2 cut(s)	296, 769
TspGWI	ACGGA	6 cut(s)	124, 245, 278, 851, 1784, 2395
TspRI	CASTG	6 cut(s)	404, 457, 1320, 1342, 1669, 2321
Tth111I	GACNNNGTC	1 cut(s)	266
Vha464I	CTTAAG	1 cut(s)	2144
VpaK11BI	GGWCC	2 cut(s)	96, 758
VspI	ATTAAT	1 cut(s)	1320
XapI	RAATTY	5 cut(s)	57, 1389, 1543, 1704, 2019
XbaI	TCTAGA	1 cut(s)	1979
XceI	RCATGY	5 cut(s)	469, 637, 693, 1850, 1938
XcmI	CCANNNNNNNNNTGG	2 cut(s)	622, 2497
XmiI	GTMKAC	2 cut(s)	138, 1725
XmnI	GAANNNNTTC	2 cut(s)	350, 2204
XspI	CTAG	8 cut(s)	11, 657, 699, 902, 1251, 1980, 2127, 2331
ZraI	GACGTC	1 cut(s)	265
Zsp2I	ATGCAT	2 cut(s)	691, 1852

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.