Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000006

Orthogroup ID

OG0001672

Physical Location & Seq

Genomic Coordinates Reverse (-)

20958334 .. 20965662

Gene Structure 7329 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_6G00399060.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1968 bp

ATGACATGCTGCGTGCAGCTGTGTGTAATTTTTATCCCTCCTAGTCGGTCAACATATAACAACTTGATCAATGCATGTGGATCTAGTGGAAATTGGAGAGAAGCTTTAAAAGTTTGCAAGAAAATGACAGATAATGGAGTTGGACCTGATCTTGTGACGCACAATATTGTTTTATCTGCATACAAAACTGGGGCTCAATATTCAAAAGCTTTATCTTATTTTGAACTTATGAAGGGAACAAATATCCGTCCAGACACAACGACCCTCAATATTGTTATATATTGCCTAGTGAAACTTGGACAGTATGGGAAAGCCATTGATATTTTCAATTCCATGAGAGATAAGGGAGCAGAATGTCGCCCTGACATCGTAACATTCACCAGCATCATTCATTTGTATTCTGTGAGCGGGCAGATAGACAACTGCACAGCTGTATTTAATACAATTCTTGCAGAAGGGCTAAAACCTAACATTGTTTCCTATAATGCACTATTAGGTGCATATGCTTCACATGGGATGTGCACAGAGGCGGCATCAGTATTTAATGAGATGAAGAAACGTAGTTTTCGGCCAGATGTTGTGTCTTATACATCTCTACTCAATGCTTATGGAAGATCACGACATCCTAAGAAGGCTAGGGAAGTTTTTGACAGGATGAAGGCCAACCACTTGAAGCCAAACCTTGTGAGCTACAATGCACTGATTAATGCCTATGGATCCAGTGGTTTATTAGCCGAAGCTGTTGAGGTCTTGCGTGAGATGGAGCAAGATGGAATTCATCCAAACATTGTCTCAATATGTACCCTTTTGGCTGCTTGTGGACAATGTGGCCAGAAGGTGAAAATTGATGCCATACTTTCAGCAGCCAAGCAGCGAGGCATTGAATTGAACACAATTGCCTATAATTCGGCTATTGGGAGCTATATGAATTTGGGAGAACATGAGAAAGCTATAAACTTGTATCAATTTATGAGAAAGAAGAAAGTGAAACCGGATTCTGTTACTTACAATGTGTTGATAAGTGGTTGTTGTAAGATGTCAAAATACAGTGAGGCACTAACCTTTTTTGATGAAATGGTGGATTTGAAAATTCCCATGTCCAAAGAAGTCTACTCATCTGTGCTATGTGCCTACAACAAGAAGGGCCAACTTACGGAAGCGGAATCTGTATTCAACTCAATGAAGATGGCTGGATGTCCTCCTGATGTAGTTACATATACTGCAATGTTACATGCGTATAGTGCTGCAGATAATTGGGAAAAGGCTTGTGAGCTATTTCAAGAAATGGAAATAAATGGCATCCAACCAGATGCTATCGCATGTTCAGCTTTGATGAGAGCTTTTAACAAAGGAGGCCATCCCTCTAGAGTTCTTATTCTGGCAGAACTCATGAGAGAAAAAGAAATTCCTTTCAATGATGCCATTTTCTTTGAAATGGTTTCGGCTTGTAGCTTATTACAAGATTGGAGAACAACAATACAATTGATTAAGGTAATAGAACCCTCATTCCCTAGACTTTCAGTCGGGCTTAAGAATCAGCTTCTGCAGATTATGGGAAGAAGCGGAAAGACAGAGGCTATGATGAAGTTGTTCTACAAGATAATTGCATCCAATGGTGATATCAACTTTGATACTTATTCAATTTTGTTGAAGAATCTTTTGTCTGTTGGAAGTTGGAGGAAATATATTGAGGTATTGCAGTGGATGGTGGATGCTAGAGTACGACCTTCAAGCCAAATGTTTCTCGATATATCCTGTTTTGCACAGAAAAGTGGTGGAACTGAATATGCTTCTATTATAAATGAAAGAATCGAATTATTGAAAAGAAAAGCAAGGGAAGAAGACTCTCGAAGCAAGCTACGTGATACTCACTCCACCTCGTTGCTGATACATAGAACAAGAGCAGAGAACCACAGGCTTCTTGAATCAAGAATGGCTATTACGCATCCTGTAGTTCCTGACTGCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003676 GO:0003723 GO:0003735 GO:0003824 GO:0004518 GO:0004519 GO:0005198 GO:0005488 GO:0006139 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0008380 GO:0009451 GO:0009790 GO:0009791 GO:0009793 GO:0009987 GO:0010154 GO:0010239 GO:0010467 GO:0016070 GO:0016071 GO:0016787 GO:0016788 GO:0022414 GO:0031425 GO:0032501 GO:0032502 GO:0034641 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0046483 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0061458 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_2 PPR_3

Protein Analysis

655

Amino Acids

73.19

Weight (kDa)

8.83

Isoelectric Point (pI)

38.3

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	14 - 61	5.1e-11	PPR repeat family
TPR_24	PF23276	17 - 112	1.4e-08	Fungal tetratrico peptide repeats
PPR_3	PF13812	17 - 61	3.6e-07	Pentatricopeptide repeat domain
PPR	PF01535	17 - 47	1.3e-07	PPR repeat
PPR_long	PF17177	105 - 201	5.1e-07	Pentacotripeptide-repeat region of PRORP
PPR_3	PF13812	108 - 168	1.8e-10	Pentatricopeptide repeat domain
PPR_2	PF13041	121 - 161	5.6e-06	PPR repeat family
PPR_1	PF12854	153 - 185	7.7e-06	PPR repeat
PPR_2	PF13041	156 - 203	1.1e-15	PPR repeat family
PPR	PF01535	159 - 188	9.9e-08	PPR repeat
PPR_long	PF17177	176 - 289	3.5e-10	Pentacotripeptide-repeat region of PRORP
PPR_3	PF13812	179 - 239	6.3e-19	Pentatricopeptide repeat domain
PPR_1	PF12854	189 - 220	9e-06	PPR repeat
PPR_2	PF13041	191 - 237	6.9e-13	PPR repeat family
PPR	PF01535	194 - 222	8.4e-07	PPR repeat
TPR_24	PF23276	199 - 271	1.2e-06	Fungal tetratrico peptide repeats
PPR_1	PF12854	223 - 255	8.8e-10	PPR repeat
PPR_2	PF13041	226 - 273	2e-11	PPR repeat family
PPR	PF01535	229 - 259	1.6e-08	PPR repeat
PPR_3	PF13812	285 - 345	1.3e-10	Pentatricopeptide repeat domain
PPR_2	PF13041	297 - 345	1.3e-16	PPR repeat family
PPR_long	PF17177	314 - 458	4.2e-14	Pentacotripeptide-repeat region of PRORP
PPR_1	PF12854	328 - 360	2e-10	PPR repeat
PPR_2	PF13041	331 - 374	8.4e-10	PPR repeat family
PPR	PF01535	334 - 362	1.6e-08	PPR repeat
PPR_3	PF13812	356 - 413	4.6e-07	Pentatricopeptide repeat domain
PPR_2	PF13041	370 - 413	1e-11	PPR repeat family
PPR_1	PF12854	398 - 430	8.3e-08	PPR repeat
PPR_2	PF13041	401 - 449	3.7e-13	PPR repeat family
PPR	PF01535	404 - 434	1.9e-07	PPR repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0001672)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G41720 AT2G41720 AT2G41720
fragaria_vesca	FvH4_6g24060 FvH4_6g24060 FvH4_6g24060 FvH4_6g24060 FvH4_6g24060 FvH4_6g24060 FvH4_6g24060 FvH4_6g24060 FvH4_6g24060 FvH4_6g24060
malus_domestica	MD12G1080100.v1.1
prunus_persica	Prupe.7G058400_v2.0.a1 Prupe.7G058400_v2.0.a1 Prupe.7G058400_v2.0.a1
rosa_chinensis	RchiOBHm_Chr3g0482651
rosa_laevigata	RLG00000001090 RLG00000016982 RLG00000023335 RLG00000028152 RLG00000032501
rosa_multiflora	Rmu_sc0004946.1_g000004 Rmu_sc0006069.1_g000004 Rmu_sc0015893.1_g000010
rosa_roxburghii	Rroxscaffold_3G00229260 Rroxscaffold_3G00244090 Rroxscaffold_4G00320230 Rroxscaffold_6G00399060 Rroxscaffold_7G00168600
rosa_rugosa	Rorug03G0197600 Rorug03G0197700
rosa_samantha	Rh3AG051200 Rh3AG249000 Rh3BG052500 Rh3BG284300 Rh3CG052100 Rh3CG282200 Rh3DG052800 Rh3DG278200 Rh4BG176200 Rh6AG132300 Rh7AG450400 Rh7BG246700 Rh7BG421800 Rh7BG421900 Rh7CG470100
rosa_wichuraiana	Rw3G022490

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1799
AccBSI	CCGCTC	1 cut(s)	408
AccI	GTMKAC	1 cut(s)	1110
AciI	CCGC	4 cut(s)	408, 530, 1160, 1563
AclWI	GGATC	3 cut(s)	88, 711, 724
AcoI	YGGCCR	2 cut(s)	569, 829
AcsI	RAATTY	4 cut(s)	774, 928, 1089, 1404
AfaI	GTAC	2 cut(s)	802, 1722
AfiI	CCNNNNNNNGG	1 cut(s)	1153
AflII	CTTAAG	1 cut(s)	1529
AhdI	GACNNNNNGTC	1 cut(s)	1520
AloI	GAACNNNNNNTCC	2 cut(s)	1491, 1523
Alw21I	GWGCWC	1 cut(s)	524
Alw26I	GTCTC	1 cut(s)	796
Alw44I	GTGCAC	1 cut(s)	520
AlwI	GGATC	3 cut(s)	88, 711, 724
AlwNI	CAGNNNCTG	1 cut(s)	1543
AoxI	GGCC	5 cut(s)	569, 660, 829, 1144, 1354
ApaLI	GTGCAC	1 cut(s)	520
ApeKI	GCWGC	6 cut(s)	9, 16, 812, 863, 871, 1244
ApoI	RAATTY	4 cut(s)	774, 928, 1089, 1404
AseI	ATTAAT	1 cut(s)	705
Asp700I	GAANNNNTTC	1 cut(s)	1589
AspS9I	GGNCC	2 cut(s)	143, 1144
AsuHPI	GGTGA	3 cut(s)	370, 850, 1628
AvaII	GGWCC	1 cut(s)	143
BaeGI	GKGCMC	1 cut(s)	524
BalI	TGGCCA	1 cut(s)	831
BamHI	GGATCC	1 cut(s)	716
BanII	GRGCYC	1 cut(s)	196
BarI	GAAGNNNNNNTAC	2 cut(s)	1577, 1609
BbsI	GAAGAC	1 cut(s)	1848
Bbv12I	GWGCWC	1 cut(s)	524
BbvI	GCAGC	5 cut(s)	28, 799, 875, 883, 1231
BccI	CCATC	5 cut(s)	754, 764, 1180, 1365, 1699
BclI	TGATCA	1 cut(s)	66
BcoDI	GTCTC	1 cut(s)	796
BfaI	CTAG	7 cut(s)	42, 84, 287, 636, 1365, 1512, 1716
BfmI	CTRYAG	3 cut(s)	1245, 1544, 1950
BfrI	CTTAAG	1 cut(s)	1529
BisI	GCNGC	7 cut(s)	10, 17, 531, 813, 864, 872, 1245
BlsI	GCNGC	7 cut(s)	11, 18, 532, 814, 865, 873, 1246
Bme18I	GGWCC	1 cut(s)	143
BmeRI	GACNNNNNGTC	1 cut(s)	1520
BmgT120I	GGNCC	2 cut(s)	143, 1144
BmiI	GGNNCC	1 cut(s)	718
BmrI	ACTGGG	1 cut(s)	198
BmsI	GCATC	9 cut(s)	393, 542, 838, 1300, 1308, 1408, 1616, 1702, 1954
BmuI	ACTGGG	1 cut(s)	198
BpiI	GAAGAC	1 cut(s)	1848
BsaAI	YACGTR	1 cut(s)	1862
BsaWI	WCCGGW	1 cut(s)	991
Bsc4I	CCNNNNNNNGG	1 cut(s)	1153
Bse1I	ACTGG	2 cut(s)	193, 720
Bse3DI	GCAATG	1 cut(s)	1230
BseLI	CCNNNNNNNGG	1 cut(s)	1153
BseMI	GCAATG	1 cut(s)	1230
BseNI	ACTGG	2 cut(s)	193, 720
BseSI	GKGCMC	1 cut(s)	524
BseXI	GCAGC	5 cut(s)	28, 799, 875, 883, 1231
BsgI	GTGCAG	2 cut(s)	35, 409
BshFI	GGCC	5 cut(s)	571, 662, 831, 1146, 1356
BsiHKAI	GWGCWC	1 cut(s)	524
BsiSI	CCGG	1 cut(s)	992
BslI	CCNNNNNNNGG	1 cut(s)	1153
BsmAI	GTCTC	1 cut(s)	796
BsnI	GGCC	5 cut(s)	571, 662, 831, 1146, 1356
Bsp1286I	GDGCHC	2 cut(s)	196, 524
Bsp143I	GATC	5 cut(s)	66, 80, 148, 614, 716
BspACI	CCGC	4 cut(s)	408, 530, 1160, 1563
BspANI	GGCC	5 cut(s)	571, 662, 831, 1146, 1356
BspHI	TCATGA	1 cut(s)	1389
BspLI	GGNNCC	1 cut(s)	718
BspMAI	CTGCAG	2 cut(s)	1249, 1548
BspPI	GGATC	3 cut(s)	88, 711, 724
BspTI	CTTAAG	1 cut(s)	1529
BsrBI	CCGCTC	1 cut(s)	408
BsrDI	GCAATG	1 cut(s)	1230
BsrI	ACTGG	2 cut(s)	193, 720
BssMI	GATC	5 cut(s)	66, 80, 148, 614, 716
Bst4CI	ACNGT	2 cut(s)	303, 1049
BstAFI	CTTAAG	1 cut(s)	1529
BstBAI	YACGTR	1 cut(s)	1862
BstC8I	GCNNGC	3 cut(s)	14, 410, 1856
BstDEI	CTNAG	1 cut(s)	627
BstKTI	GATC	5 cut(s)	69, 83, 151, 617, 719
BstMAI	GTCTC	1 cut(s)	796
BstMBI	GATC	5 cut(s)	66, 80, 148, 614, 716
BstMWI	GCNNNNNNNGC	2 cut(s)	1241, 1325
BstNSI	RCATGY	4 cut(s)	9, 78, 1235, 1323
BstSFI	CTRYAG	3 cut(s)	1245, 1544, 1950
BstSLI	GKGCMC	1 cut(s)	524
BstV1I	GCAGC	5 cut(s)	28, 799, 875, 883, 1231
BstV2I	GAAGAC	1 cut(s)	1848
BstX2I	RGATCY	2 cut(s)	80, 716
BstYI	RGATCY	2 cut(s)	80, 716
BsuRI	GGCC	5 cut(s)	571, 662, 831, 1146, 1356
BtsI	GCAGTG	1 cut(s)	1706
BtsIMutI	CAGTG	4 cut(s)	698, 727, 1054, 1706
Cac8I	GCNNGC	3 cut(s)	14, 410, 1856
CaiI	CAGNNNCTG	1 cut(s)	1543
CciI	TCATGA	1 cut(s)	1389
Cfr13I	GGNCC	2 cut(s)	143, 1144
CseI	GACGC	1 cut(s)	166
Csp6I	GTAC	2 cut(s)	801, 1721
CspCI	CAANNNNNGTGG	2 cut(s)	58, 93
CviAII	CATG	9 cut(s)	6, 75, 334, 512, 941, 1096, 1232, 1320, 1390
CviQI	GTAC	2 cut(s)	801, 1721
DdeI	CTNAG	1 cut(s)	627
DpnI	GATC	5 cut(s)	68, 82, 150, 616, 718
DpnII	GATC	5 cut(s)	66, 80, 148, 614, 716
DraI	TTTAAA	1 cut(s)	108
DriI	GACNNNNNGTC	1 cut(s)	1520
EaeI	YGGCCR	2 cut(s)	569, 829
Eam1105I	GACNNNNNGTC	1 cut(s)	1520
Eco24I	GRGCYC	1 cut(s)	196
Eco32I	GATATC	1 cut(s)	1621
Eco47I	GGWCC	1 cut(s)	143
EcoRI	GAATTC	1 cut(s)	774
EcoRV	GATATC	1 cut(s)	1621
EcoT22I	ATGCAT	1 cut(s)	76
EcoT38I	GRGCYC	1 cut(s)	196
FaeI	CATG	9 cut(s)	9, 78, 337, 515, 944, 1099, 1235, 1323, 1393
FatI	CATG	9 cut(s)	5, 74, 333, 511, 940, 1095, 1231, 1319, 1389
FauI	CCCGC	1 cut(s)	401
FauNDI	CATATG	1 cut(s)	502
FbaI	TGATCA	1 cut(s)	66
FblI	GTMKAC	1 cut(s)	1110
Fnu4HI	GCNGC	7 cut(s)	10, 17, 531, 813, 864, 872, 1245
FriOI	GRGCYC	1 cut(s)	196
Fsp4HI	GCNGC	7 cut(s)	10, 17, 531, 813, 864, 872, 1245
FspBI	CTAG	7 cut(s)	42, 84, 287, 636, 1365, 1512, 1716
GluI	GCNGC	7 cut(s)	10, 17, 531, 813, 864, 872, 1245
HaeIII	GGCC	5 cut(s)	571, 662, 831, 1146, 1356
HapII	CCGG	1 cut(s)	992
HgaI	GACGC	1 cut(s)	166
Hin1II	CATG	9 cut(s)	9, 78, 337, 515, 944, 1099, 1235, 1323, 1393
HincII	GTYRAC	1 cut(s)	51
HindII	GTYRAC	1 cut(s)	51
HindIII	AAGCTT	2 cut(s)	102, 207
HinfI	GANTC	7 cut(s)	995, 1163, 1534, 1654, 1809, 1844, 1925
HpaII	CCGG	1 cut(s)	992
HphI	GGTGA	3 cut(s)	370, 850, 1628
Hpy166II	GTNNAC	4 cut(s)	51, 522, 821, 1111
Hpy8I	GTNNAC	4 cut(s)	51, 522, 821, 1111
HpyAV	CCTTC	7 cut(s)	226, 449, 625, 652, 829, 1135, 1737
HpyCH4III	ACNGT	2 cut(s)	303, 1049
HpyCH4IV	ACGT	2 cut(s)	559, 1861
HpyF10VI	GCNNNNNNNGC	2 cut(s)	1241, 1325
HpyF3I	CTNAG	1 cut(s)	627
HpySE526I	ACGT	2 cut(s)	559, 1861
Hsp92II	CATG	9 cut(s)	9, 78, 337, 515, 944, 1099, 1235, 1323, 1393
Ksp22I	TGATCA	1 cut(s)	66
Kzo9I	GATC	5 cut(s)	66, 80, 148, 614, 716
LmnI	GCTCC	3 cut(s)	347, 763, 918
Lsp1109I	GCAGC	5 cut(s)	28, 799, 875, 883, 1231
LweI	GCATC	9 cut(s)	393, 542, 838, 1300, 1308, 1408, 1616, 1702, 1954
MaeI	CTAG	7 cut(s)	42, 84, 287, 636, 1365, 1512, 1716
MaeII	ACGT	2 cut(s)	559, 1861
MaeIII	GTNAC	5 cut(s)	154, 370, 1000, 1210, 1227
MalI	GATC	5 cut(s)	68, 82, 150, 616, 718
MbiI	CCGCTC	1 cut(s)	408
MboI	GATC	5 cut(s)	66, 80, 148, 614, 716
MboII	GAAGA	8 cut(s)	565, 624, 991, 1195, 1569, 1663, 1850, 1853
MfeI	CAATTG	2 cut(s)	894, 1481
MflI	RGATCY	2 cut(s)	80, 716
MhlI	GDGCHC	2 cut(s)	196, 524
MlsI	TGGCCA	1 cut(s)	831
MluNI	TGGCCA	1 cut(s)	831
MlyI	GAGTC	1 cut(s)	1838
MmeI	TCCRAC	4 cut(s)	121, 1327, 1648, 1655
Mox20I	TGGCCA	1 cut(s)	831
Mph1103I	ATGCAT	1 cut(s)	76
MroXI	GAANNNNTTC	1 cut(s)	1589
MscI	TGGCCA	1 cut(s)	831
MseI	TTAA	7 cut(s)	107, 438, 543, 705, 1344, 1488, 1530
Msp20I	TGGCCA	1 cut(s)	831
MspA1I	CMGCKG	2 cut(s)	19, 431
MspCI	CTTAAG	1 cut(s)	1529
MspI	CCGG	1 cut(s)	992
MunI	CAATTG	2 cut(s)	894, 1481
MwoI	GCNNNNNNNGC	2 cut(s)	1241, 1325
NdeI	CATATG	1 cut(s)	502
NdeII	GATC	5 cut(s)	66, 80, 148, 614, 716
NlaIII	CATG	9 cut(s)	9, 78, 337, 515, 944, 1099, 1235, 1323, 1393
NlaIV	GGNNCC	1 cut(s)	718
NmuCI	GTSAC	1 cut(s)	154
NsiI	ATGCAT	1 cut(s)	76
NspI	RCATGY	4 cut(s)	9, 78, 1235, 1323
PagI	TCATGA	1 cut(s)	1389
PdmI	GAANNNNTTC	1 cut(s)	1589
PfeI	GAWTC	6 cut(s)	995, 1163, 1534, 1654, 1809, 1925
PkrI	GCNGC	7 cut(s)	11, 18, 532, 814, 865, 873, 1246
PleI	GAGTC	1 cut(s)	1838
PpsI	GAGTC	1 cut(s)	1838
Ppu21I	YACGTR	1 cut(s)	1862
PshBI	ATTAAT	1 cut(s)	705
PsiI	TTATAA	1 cut(s)	1799
PspN4I	GGNNCC	1 cut(s)	718
PspPI	GGNCC	2 cut(s)	143, 1144
PstI	CTGCAG	2 cut(s)	1249, 1548
PstNI	CAGNNNCTG	1 cut(s)	1543
PsuI	RGATCY	2 cut(s)	80, 716
PvuII	CAGCTG	2 cut(s)	19, 431
RsaI	GTAC	2 cut(s)	802, 1722
RsaNI	GTAC	2 cut(s)	801, 1721
SaqAI	TTAA	7 cut(s)	107, 438, 543, 705, 1344, 1488, 1530
SatI	GCNGC	7 cut(s)	10, 17, 531, 813, 864, 872, 1245
Sau3AI	GATC	5 cut(s)	66, 80, 148, 614, 716
Sau96I	GGNCC	2 cut(s)	143, 1144
SchI	GAGTC	1 cut(s)	1838
SduI	GDGCHC	2 cut(s)	196, 524
SfaNI	GCATC	9 cut(s)	393, 542, 838, 1300, 1308, 1408, 1616, 1702, 1954
SfcI	CTRYAG	3 cut(s)	1245, 1544, 1950
SinI	GGWCC	1 cut(s)	143
SmlI	CTYRAG	1 cut(s)	1529
SmoI	CTYRAG	1 cut(s)	1529
SsiI	CCGC	4 cut(s)	408, 530, 1160, 1563
SspI	AATATT	3 cut(s)	166, 200, 271
SspMI	CTAG	7 cut(s)	42, 84, 287, 636, 1365, 1512, 1716
TaaI	ACNGT	2 cut(s)	303, 1049
TaiI	ACGT	2 cut(s)	562, 1864
TaqI	TCGA	3 cut(s)	1746, 1812, 1849
TaqII	GACCGA	1 cut(s)	36
TauI	GCSGC	1 cut(s)	533
TfiI	GAWTC	6 cut(s)	995, 1163, 1534, 1654, 1809, 1925
Tru1I	TTAA	7 cut(s)	107, 438, 543, 705, 1344, 1488, 1530
Tru9I	TTAA	7 cut(s)	107, 438, 543, 705, 1344, 1488, 1530
TscAI	CASTG	4 cut(s)	705, 727, 1054, 1706
TseFI	GTSAC	1 cut(s)	154
TseI	GCWGC	6 cut(s)	9, 16, 812, 863, 871, 1244
Tsp45I	GTSAC	1 cut(s)	154
TspGWI	ACGGA	2 cut(s)	236, 1169
TspRI	CASTG	4 cut(s)	705, 727, 1054, 1706
Vha464I	CTTAAG	1 cut(s)	1529
VneI	GTGCAC	1 cut(s)	520
VpaK11BI	GGWCC	1 cut(s)	143
VspI	ATTAAT	1 cut(s)	705
XapI	RAATTY	4 cut(s)	774, 928, 1089, 1404
XbaI	TCTAGA	1 cut(s)	1364
XceI	RCATGY	4 cut(s)	9, 78, 1235, 1323
XmiI	GTMKAC	1 cut(s)	1110
XmnI	GAANNNNTTC	1 cut(s)	1589
XspI	CTAG	7 cut(s)	42, 84, 287, 636, 1365, 1512, 1716
Zsp2I	ATGCAT	1 cut(s)	76

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.