Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr3C

Orthogroup ID

OG0001672

Physical Location & Seq

Genomic Coordinates Forward (+)

28405332 .. 28412445

Gene Structure 7114 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh3CG282200.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2592 bp

ATGCACACAATCCCAATGGCCACTCTAGTATCCCAGCCACCCCTCCTCCACCTTCCGCAGTCCAAGACCCGAATTCTCTGCAACAAGCCCAACAACAACAAGAAGACCGACTCAGCAGCATTCAAGGAGAAGCAGCACGTCTCCGTCGACTACGACAGCGGCACTCACCACCTCTCCACTCATATCGGCGGCTTCAGGAAGCGAGACATCCCCAAACACCACCGCTTACGCGTCGAAACGGAGCGTTTCCAGAAGGACTGGGCCGTCTCCGACGTCGTCAATCGGGTCTATCAGCTCCGTCGCGGTGACGACATTGACGGCGTGCTCAATCGATGGGTCGGACGCTTCGCCAGGAAGAACTTCCCCATCCTAATTAGAGAAATGACGGCGAGGGGGTCGGTGGAGCACTGTATGCAAGTGTTCCGGTGGATGAAGACCCTCAAGAACTACTGTGCTCGCACTGATATTTATAACATGTTGATCAGATTGCACGCCAGGCATAGTCTTGTTGATCAAGCTCGCGGCTTGTTTTTCGAAATGCAAGAATGGAGGTGCAAGCCTGATGCTGAGTCCTACAATGCTCTTATCAATGCACATGGTCGAGCAGGTCAATGGCGCTGGGCCATGAACATTATGGATGACATGCTGCGTGCAGCTATCCCTCCTAGTCGGTCAACATATAACAACTTGATCAATGCATGTGGATCTAGTGGAAATTGGAGAGAAGCTTTAAAAGTTTGCAAGAAAATGACAGATAATGGAGTTGGACCTGATCTTGTGACTCACAATATTGTTTTATCTGCATACAAAACTGGGGCTCAATATTCAAAAGCTTTATCTTATTTTGAACTTATGAAGGGAACAAATATCCGTCCAGACACAACGACCCTCAATATTGTTATATATTGCCTAGTGAAGCTTGGACAGTATGGGAAAGCCATTGATATTTTCAATTCCATGAGAGATAAGGGAGCAGAATGTCGCCCTGACATCGTAACATTCACCAGCATCATTCATTTGTATTCTGTGAGCGGGCAGATAGACAACTGCACAGCCGTATTTAATACAATTCTTGCAGAAGGGCTAAAACCTAACATTGTTTCCTATAATGCACTATTAGGTGCATATGCTTCACATGGGATGTGCACAGAGGCGGCATCAGTATTTAATGAGATGAAGAAAAGTAGTTTTCGGGCAGATGTTGTGTCGTATACATCTCTACTCAATGCTTATGGAAGATCACGACATCCTAAGAAGGCTAGGGAAGTTTTTGACAGGATGAAGGCCAACCACTTGAAGCCAAACCTTGTGAGCTACAACGCACTGATTAATGCCTATGGATCCAGTGGTTTATTAGCCGAAGCTGTTGAGGTCTTGCGTGAGATGGAGCAAAATGGAATTCATCCAAACATTGTCTCAATATGTACCCTTTTGGCTGCTTGCGGACAATGTGGCCAGAAGGTGAAAATTGATGCCATACTTTCAGCAGCCAAGCTACGAGGCATTGAATTGAACACAATTGCCTATAATTCGGCTATTGGGAGCTATATGAATTTGGGAGAACATGAGAAAGCTATAAACTTGTATCAATCTATGAGAAAGAACAAAGTGAAACCGGATTCTGTTACTTACAATGTGTTGATAAGTGGTTGTTGTAAGATGTCAAAATACAGTGAGGCACTAACCTTTTTTGATGAAATGGTGGATTTGAAAATTCCTATGTCCAAAGAAGTCTACTCATCTGTGCTCTGTGCCTACAACAAGAAGGGCCAACTTACGGAAGCGGAATCTGTATTCAACTCAATGAAGATGGCTGGATGTCCTCCTGATGTAGTTACATATACTGCAATGTTACATGCGTATAGTGCTGCAGAGAATTGGGAAAAGGCTTGTCAGCTATTTCAAGAAATGGAAATAAATGGCATCCAACCAGATGCTATCGCATGTTCAGCTCTGATGAGAGCTTTTAACAAAGGAGGCCATCCCTCTAGAGTTCTTTTTCTGGCAGAACTCATGAGAGAAAAAGAAATTCCTTTCAATGATGCCATTTTCTTTGAAATGGTTTCGGCTTGTAGCTTATTACAAGATTGGAGAACAACAATACAATTGATTAAGGTAATAGAGCCCTCATTCCCTAGACTTTCAGTCGGGCTTAAGAATCAGCTTCTGCAGATTATGGGAAGAAGCGGAAAGACAGAGGCTATGATGAAGTTGTTCTACAAGATAATTGCATCCAATGGTGATATCAACTTTGATACTTACTCAATTTTGTTGAAGAATCTTTTGTCTGTTGGAAGTTGGAGGAAATATATTGAGGTATTGCAGTGGATGGTGGATGCTAGAGTACGACCTTCAAGCCAAATGTATCTCGATATATCCTATTTTGCACAGAAAAGTGGTGGAACTGAATATGCTTCTATTATAAATGAAAGAATCGAATTATTGAAAAGAAAAGCAAGGGAAGAAGACTCTCGAAGCAAGCTACGTGATACTCACTCCACTTCCATGCTGGTACATAGAACAAGAGCAGAGAACCACAGGCTGCTTGAATCAAGAATGGCTGTTACGCATCCTGTAGTTCCTGACTGCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003676 GO:0003723 GO:0003735 GO:0003824 GO:0004518 GO:0004519 GO:0005198 GO:0005488 GO:0006139 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0008380 GO:0009451 GO:0009790 GO:0009791 GO:0009793 GO:0009987 GO:0010154 GO:0010239 GO:0010467 GO:0016070 GO:0016071 GO:0016787 GO:0016788 GO:0022414 GO:0031425 GO:0032501 GO:0032502 GO:0034641 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0046483 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0061458 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_2 PPR_3

Protein Analysis

863

Amino Acids

97.61

Weight (kDa)

9.25

Isoelectric Point (pI)

38.51

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	156 - 196	4.4e-08	PPR repeat family
PPR_3	PF13812	177 - 235	6e-08	Pentatricopeptide repeat domain
PPR_2	PF13041	187 - 234	5.7e-11	PPR repeat family
TPR_24	PF23276	200 - 320	9.4e-10	Fungal tetratrico peptide repeats
PPR_long	PF17177	208 - 322	3.9e-08	Pentacotripeptide-repeat region of PRORP
PPR_3	PF13812	211 - 269	5.4e-09	Pentatricopeptide repeat domain
PPR_2	PF13041	222 - 269	7e-11	PPR repeat family
PPR	PF01535	225 - 255	1.7e-07	PPR repeat
PPR_long	PF17177	313 - 408	2.1e-06	Pentacotripeptide-repeat region of PRORP
PPR_3	PF13812	316 - 376	2.6e-10	Pentatricopeptide repeat domain
PPR_2	PF13041	329 - 369	7.9e-06	PPR repeat family
PPR_2	PF13041	364 - 411	1.4e-13	PPR repeat family
PPR	PF01535	367 - 396	2e-07	PPR repeat
PPR_long	PF17177	384 - 495	1e-09	Pentacotripeptide-repeat region of PRORP
PPR_3	PF13812	387 - 447	8.4e-18	Pentatricopeptide repeat domain
PPR_2	PF13041	400 - 445	4.6e-12	PPR repeat family
PPR	PF01535	402 - 430	1.2e-06	PPR repeat
TPR_24	PF23276	406 - 531	4.7e-11	Fungal tetratrico peptide repeats
PPR_1	PF12854	431 - 463	1.2e-09	PPR repeat
PPR_2	PF13041	434 - 481	9.7e-12	PPR repeat family
PPR	PF01535	437 - 467	1.7e-08	PPR repeat
TPR_24	PF23276	477 - 601	2.9e-06	Fungal tetratrico peptide repeats
PPR_3	PF13812	493 - 553	2.7e-10	Pentatricopeptide repeat domain
PPR_2	PF13041	505 - 553	7.9e-17	PPR repeat family
PPR_long	PF17177	522 - 666	1.8e-14	Pentacotripeptide-repeat region of PRORP
PPR_1	PF12854	537 - 568	3.6e-10	PPR repeat
PPR_2	PF13041	540 - 583	7.5e-09	PPR repeat family
PPR	PF01535	542 - 570	2.3e-08	PPR repeat
PPR_3	PF13812	564 - 621	6.5e-07	Pentatricopeptide repeat domain
PPR_2	PF13041	578 - 621	1.4e-11	PPR repeat family
PPR_1	PF12854	606 - 638	1.3e-07	PPR repeat
PPR_2	PF13041	609 - 657	5.6e-13	PPR repeat family
PPR	PF01535	612 - 642	9.6e-07	PPR repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0001672)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G41720 AT2G41720 AT2G41720
fragaria_vesca	FvH4_6g24060 FvH4_6g24060 FvH4_6g24060 FvH4_6g24060 FvH4_6g24060 FvH4_6g24060 FvH4_6g24060 FvH4_6g24060 FvH4_6g24060 FvH4_6g24060
malus_domestica	MD12G1080100.v1.1
prunus_persica	Prupe.7G058400_v2.0.a1 Prupe.7G058400_v2.0.a1 Prupe.7G058400_v2.0.a1
rosa_chinensis	RchiOBHm_Chr3g0482651
rosa_laevigata	RLG00000001090 RLG00000016982 RLG00000023335 RLG00000028152 RLG00000032501
rosa_multiflora	Rmu_sc0004946.1_g000004 Rmu_sc0006069.1_g000004 Rmu_sc0015893.1_g000010
rosa_roxburghii	Rroxscaffold_3G00229260 Rroxscaffold_3G00244090 Rroxscaffold_4G00320230 Rroxscaffold_6G00399060 Rroxscaffold_7G00168600
rosa_rugosa	Rorug03G0197600 Rorug03G0197700
rosa_samantha	Rh3AG051200 Rh3AG249000 Rh3BG052500 Rh3BG284300 Rh3CG052100 Rh3CG282200 Rh3DG052800 Rh3DG278200 Rh4BG176200 Rh6AG132300 Rh7AG450400 Rh7BG246700 Rh7BG421800 Rh7BG421900 Rh7CG470100
rosa_wichuraiana	Rw3G022490

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	471, 2423
AasI	GACNNNNNNGTC	1 cut(s)	263
AatII	GACGTC	1 cut(s)	276
Acc36I	ACCTGC	1 cut(s)	596
AccBSI	CCGCTC	1 cut(s)	1032
AccI	GTMKAC	3 cut(s)	147, 1211, 1734
AccII	CGCG	3 cut(s)	231, 303, 522
AclWI	GGATC	3 cut(s)	712, 1335, 1348
AcoI	YGGCCR	2 cut(s)	18, 1453
AcsI	RAATTY	5 cut(s)	72, 1398, 1552, 1713, 2028
AcuI	CTGAAG	1 cut(s)	178
AcyI	GRCGYC	1 cut(s)	273
AfaI	GTAC	3 cut(s)	1426, 2346, 2514
AfiI	CCNNNNNNNGG	1 cut(s)	1777
AflII	CTTAAG	1 cut(s)	2153
AflIII	ACRYGT	2 cut(s)	229, 474
AhdI	GACNNNNNGTC	1 cut(s)	2144
AjiI	CACGTC	1 cut(s)	139
AjnI	CCWGG	2 cut(s)	350, 494
Alw21I	GWGCWC	5 cut(s)	327, 408, 457, 1148, 1749
Alw26I	GTCTC	4 cut(s)	145, 198, 271, 1420
Alw44I	GTGCAC	1 cut(s)	1144
AlwI	GGATC	3 cut(s)	712, 1335, 1348
AlwNI	CAGNNNCTG	1 cut(s)	2167
AoxI	GGCC	7 cut(s)	18, 261, 621, 1284, 1453, 1768, 1978
ApaLI	GTGCAC	1 cut(s)	1144
ApeKI	GCWGC	8 cut(s)	116, 133, 646, 653, 1436, 1487, 1868, 2542
ApoI	RAATTY	5 cut(s)	72, 1398, 1552, 1713, 2028
AseI	ATTAAT	1 cut(s)	1329
Asp700I	GAANNNNTTC	2 cut(s)	359, 2213
AspLEI	GCGC	1 cut(s)	618
AspS9I	GGNCC	4 cut(s)	261, 621, 767, 1768
AsuHPI	GGTGA	5 cut(s)	158, 317, 994, 1474, 2252
AsuII	TTCGAA	1 cut(s)	534
AvaII	GGWCC	1 cut(s)	767
BaeGI	GKGCMC	1 cut(s)	1148
BaeI	ACNNNNGTAYC	2 cut(s)	12, 45
BalI	TGGCCA	2 cut(s)	20, 1455
BamHI	GGATCC	1 cut(s)	1340
BanII	GRGCYC	2 cut(s)	820, 2127
BarI	GAAGNNNNNNTAC	2 cut(s)	2201, 2233
BbsI	GAAGAC	3 cut(s)	110, 440, 2472
Bbv12I	GWGCWC	5 cut(s)	327, 408, 457, 1148, 1749
BbvI	GCAGC	8 cut(s)	128, 145, 633, 665, 1423, 1499, 1855, 2529
BccI	CCATC	6 cut(s)	327, 374, 1378, 1804, 1989, 2323
BceAI	ACGGC	4 cut(s)	248, 334, 402, 1040
BcgI	CGANNNNNNTGC	2 cut(s)	60, 94
BciT130I	CCWGG	2 cut(s)	352, 496
BciVI	GTATCC	1 cut(s)	40
BclI	TGATCA	3 cut(s)	480, 511, 690
BcoDI	GTCTC	4 cut(s)	145, 198, 271, 1420
BfaI	CTAG	8 cut(s)	26, 666, 708, 911, 1260, 1989, 2136, 2340
BfmI	CTRYAG	3 cut(s)	1869, 2168, 2574
BfoI	RGCGCY	1 cut(s)	619
BfrI	CTTAAG	1 cut(s)	2153
BfuAI	ACCTGC	1 cut(s)	596
BfuI	GTATCC	1 cut(s)	40
Bme1390I	CCNGG	2 cut(s)	352, 496
Bme18I	GGWCC	1 cut(s)	767
BmeRI	GACNNNNNGTC	1 cut(s)	2144
BmgBI	CACGTC	1 cut(s)	139
BmgT120I	GGNCC	4 cut(s)	261, 621, 767, 1768
BmiI	GGNNCC	1 cut(s)	1342
BmrFI	CCNGG	2 cut(s)	352, 496
BmrI	ACTGGG	2 cut(s)	268, 822
BmuI	ACTGGG	2 cut(s)	268, 822
BpiI	GAAGAC	3 cut(s)	110, 440, 2472
Bpu14I	TTCGAA	1 cut(s)	534
BpuEI	CTTGAG	1 cut(s)	425
Bsa29I	ATCGAT	1 cut(s)	331
BsaAI	YACGTR	1 cut(s)	2486
BsaHI	GRCGYC	1 cut(s)	273
BsaWI	WCCGGW	2 cut(s)	423, 1615
BsaXI	ACNNNNNCTCC	4 cut(s)	30, 60, 158, 188
Bsc4I	CCNNNNNNNGG	1 cut(s)	1777
Bse1I	ACTGG	3 cut(s)	263, 817, 1344
Bse3DI	GCAATG	1 cut(s)	1854
BseBI	CCWGG	2 cut(s)	352, 496
BseCI	ATCGAT	1 cut(s)	331
BseLI	CCNNNNNNNGG	1 cut(s)	1777
BseMI	GCAATG	1 cut(s)	1854
BseMII	CTCAG	2 cut(s)	126, 558
BseNI	ACTGG	3 cut(s)	263, 817, 1344
BseRI	GAGGAG	1 cut(s)	35
BseSI	GKGCMC	1 cut(s)	1148
BseXI	GCAGC	8 cut(s)	128, 145, 633, 665, 1423, 1499, 1855, 2529
BseYI	CCCAGC	2 cut(s)	33, 618
BsgI	GTGCAG	2 cut(s)	672, 1033
Bsh1236I	CGCG	3 cut(s)	231, 303, 522
BshFI	GGCC	7 cut(s)	20, 263, 623, 1286, 1455, 1770, 1980
BshVI	ATCGAT	1 cut(s)	331
BsiHKAI	GWGCWC	5 cut(s)	327, 408, 457, 1148, 1749
BsiSI	CCGG	2 cut(s)	424, 1616
BslI	CCNNNNNNNGG	1 cut(s)	1777
BsmAI	GTCTC	4 cut(s)	145, 198, 271, 1420
BsmBI	CGTCTC	2 cut(s)	145, 271
BsmI	GAATGC	1 cut(s)	119
BsnI	GGCC	7 cut(s)	20, 263, 623, 1286, 1455, 1770, 1980
Bsp119I	TTCGAA	1 cut(s)	534
Bsp1286I	GDGCHC	7 cut(s)	327, 408, 457, 820, 1148, 1749, 2127
Bsp143I	GATC	7 cut(s)	480, 511, 690, 704, 772, 1238, 1340
BspANI	GGCC	7 cut(s)	20, 263, 623, 1286, 1455, 1770, 1980
BspCNI	CTCAG	2 cut(s)	125, 559
BspDI	ATCGAT	1 cut(s)	331
BspFNI	CGCG	3 cut(s)	231, 303, 522
BspHI	TCATGA	1 cut(s)	2013
BspLI	GGNNCC	1 cut(s)	1342
BspMAI	CTGCAG	2 cut(s)	1873, 2172
BspMI	ACCTGC	1 cut(s)	596
BspPI	GGATC	3 cut(s)	712, 1335, 1348
BspT104I	TTCGAA	1 cut(s)	534
BspTI	CTTAAG	1 cut(s)	2153
BsrBI	CCGCTC	1 cut(s)	1032
BsrDI	GCAATG	1 cut(s)	1854
BsrI	ACTGG	3 cut(s)	263, 817, 1344
BssMI	GATC	7 cut(s)	480, 511, 690, 704, 772, 1238, 1340
BssNAI	GTATAC	1 cut(s)	1212
BssNI	GRCGYC	1 cut(s)	273
Bst1107I	GTATAC	1 cut(s)	1212
Bst2UI	CCWGG	2 cut(s)	352, 496
Bst4CI	ACNGT	4 cut(s)	410, 452, 927, 1673
BstACI	GRCGYC	1 cut(s)	273
BstAFI	CTTAAG	1 cut(s)	2153
BstAPI	GCANNNNNTGC	1 cut(s)	412
BstBAI	YACGTR	1 cut(s)	2486
BstBI	TTCGAA	1 cut(s)	534
BstC8I	GCNNGC	9 cut(s)	323, 457, 492, 520, 557, 651, 1034, 1441, 2480
BstDEI	CTNAG	3 cut(s)	112, 567, 1251
BstFNI	CGCG	3 cut(s)	231, 303, 522
BstH2I	RGCGCY	1 cut(s)	619
BstHHI	GCGC	1 cut(s)	618
BstKTI	GATC	7 cut(s)	483, 514, 693, 707, 775, 1241, 1343
BstMAI	GTCTC	4 cut(s)	145, 198, 271, 1420
BstMBI	GATC	7 cut(s)	480, 511, 690, 704, 772, 1238, 1340
BstMWI	GCNNNNNNNGC	4 cut(s)	412, 496, 1865, 1949
BstNI	CCWGG	2 cut(s)	352, 496
BstNSI	RCATGY	5 cut(s)	478, 646, 702, 1859, 1947
BstSCI	CCNGG	2 cut(s)	350, 494
BstSFI	CTRYAG	3 cut(s)	1869, 2168, 2574
BstSLI	GKGCMC	1 cut(s)	1148
BstUI	CGCG	3 cut(s)	231, 303, 522
BstV1I	GCAGC	8 cut(s)	128, 145, 633, 665, 1423, 1499, 1855, 2529
BstV2I	GAAGAC	3 cut(s)	110, 440, 2472
BstX2I	RGATCY	2 cut(s)	704, 1340
BstYI	RGATCY	2 cut(s)	704, 1340
BstZ17I	GTATAC	1 cut(s)	1212
Bsu15I	ATCGAT	1 cut(s)	331
BsuI	GTATCC	1 cut(s)	40
BsuRI	GGCC	7 cut(s)	20, 263, 623, 1286, 1455, 1770, 1980
BsuTUI	ATCGAT	1 cut(s)	331
BtrI	CACGTC	1 cut(s)	139
BtsI	GCAGTG	1 cut(s)	2330
BtsIMutI	CAGTG	6 cut(s)	406, 459, 1322, 1351, 1678, 2330
BveI	ACCTGC	1 cut(s)	596
Cac8I	GCNNGC	9 cut(s)	323, 457, 492, 520, 557, 651, 1034, 1441, 2480
CaiI	CAGNNNCTG	1 cut(s)	2167
CciI	TCATGA	1 cut(s)	2013
CfoI	GCGC	1 cut(s)	618
Cfr13I	GGNCC	4 cut(s)	261, 621, 767, 1768
ClaI	ATCGAT	1 cut(s)	331
CseI	GACGC	2 cut(s)	220, 351
Csp6I	GTAC	3 cut(s)	1425, 2345, 2513
CspCI	CAANNNNNGTGG	2 cut(s)	682, 717
CviQI	GTAC	3 cut(s)	1425, 2345, 2513
DdeI	CTNAG	3 cut(s)	112, 567, 1251
DpnI	GATC	7 cut(s)	482, 513, 692, 706, 774, 1240, 1342
DpnII	GATC	7 cut(s)	480, 511, 690, 704, 772, 1238, 1340
DraI	TTTAAA	1 cut(s)	732
DrdI	GACNNNNNNGTC	1 cut(s)	263
DriI	GACNNNNNGTC	1 cut(s)	2144
DseDI	GACNNNNNNGTC	1 cut(s)	263
EaeI	YGGCCR	2 cut(s)	18, 1453
Eam1105I	GACNNNNNGTC	1 cut(s)	2144
Eco24I	GRGCYC	2 cut(s)	820, 2127
Eco32I	GATATC	1 cut(s)	2245
Eco47I	GGWCC	1 cut(s)	767
Eco57I	CTGAAG	1 cut(s)	178
EcoRI	GAATTC	2 cut(s)	72, 1398
EcoRII	CCWGG	2 cut(s)	350, 494
EcoRV	GATATC	1 cut(s)	2245
EcoT22I	ATGCAT	1 cut(s)	700
EcoT38I	GRGCYC	2 cut(s)	820, 2127
Esp3I	CGTCTC	2 cut(s)	145, 271
FauI	CCCGC	1 cut(s)	1025
FauNDI	CATATG	1 cut(s)	1126
FbaI	TGATCA	3 cut(s)	480, 511, 690
FblI	GTMKAC	3 cut(s)	147, 1211, 1734
FriOI	GRGCYC	2 cut(s)	820, 2127
FspBI	CTAG	8 cut(s)	26, 666, 708, 911, 1260, 1989, 2136, 2340
GlaI	GCGC	1 cut(s)	617
GsaI	CCCAGC	2 cut(s)	37, 622
HaeII	RGCGCY	1 cut(s)	619
HaeIII	GGCC	7 cut(s)	20, 263, 623, 1286, 1455, 1770, 1980
HapII	CCGG	2 cut(s)	424, 1616
HgaI	GACGC	2 cut(s)	220, 351
HhaI	GCGC	1 cut(s)	618
Hin1I	GRCGYC	1 cut(s)	273
Hin6I	GCGC	1 cut(s)	616
HinP1I	GCGC	1 cut(s)	616
HincII	GTYRAC	2 cut(s)	148, 675
HindII	GTYRAC	2 cut(s)	148, 675
HindIII	AAGCTT	3 cut(s)	726, 831, 917
HpaII	CCGG	2 cut(s)	424, 1616
HphI	GGTGA	5 cut(s)	158, 317, 994, 1474, 2252
Hpy166II	GTNNAC	5 cut(s)	148, 675, 1146, 1212, 1735
Hpy188I	TCNGA	4 cut(s)	271, 341, 485, 1956
Hpy8I	GTNNAC	5 cut(s)	148, 675, 1146, 1212, 1735
Hpy99I	CGWCG	5 cut(s)	149, 236, 275, 278, 303
HpyAV	CCTTC	9 cut(s)	62, 247, 850, 1073, 1249, 1276, 1453, 1759, 2361
HpyCH4III	ACNGT	4 cut(s)	410, 452, 927, 1673
HpyCH4IV	ACGT	3 cut(s)	138, 273, 2485
HpyF10VI	GCNNNNNNNGC	4 cut(s)	412, 496, 1865, 1949
HpyF3I	CTNAG	3 cut(s)	112, 567, 1251
HpySE526I	ACGT	3 cut(s)	138, 273, 2485
Hsp92I	GRCGYC	1 cut(s)	273
HspAI	GCGC	1 cut(s)	616
Ksp22I	TGATCA	3 cut(s)	480, 511, 690
Kzo9I	GATC	7 cut(s)	480, 511, 690, 704, 772, 1238, 1340
LmnI	GCTCC	6 cut(s)	241, 300, 403, 971, 1387, 1542
Lsp1109I	GCAGC	8 cut(s)	128, 145, 633, 665, 1423, 1499, 1855, 2529
MaeI	CTAG	8 cut(s)	26, 666, 708, 911, 1260, 1989, 2136, 2340
MaeII	ACGT	3 cut(s)	138, 273, 2485
MaeIII	GTNAC	7 cut(s)	305, 778, 994, 1624, 1834, 1851, 2563
MalI	GATC	7 cut(s)	482, 513, 692, 706, 774, 1240, 1342
MbiI	CCGCTC	1 cut(s)	1032
MboI	GATC	7 cut(s)	480, 511, 690, 704, 772, 1238, 1340
MfeI	CAATTG	2 cut(s)	1518, 2105
MflI	RGATCY	2 cut(s)	704, 1340
MhlI	GDGCHC	7 cut(s)	327, 408, 457, 820, 1148, 1749, 2127
MlsI	TGGCCA	2 cut(s)	20, 1455
MluI	ACGCGT	1 cut(s)	229
MluNI	TGGCCA	2 cut(s)	20, 1455
MlyI	GAGTC	4 cut(s)	104, 578, 775, 2462
MmeI	TCCRAC	6 cut(s)	294, 319, 745, 1951, 2272, 2279
Mox20I	TGGCCA	2 cut(s)	20, 1455
Mph1103I	ATGCAT	1 cut(s)	700
MroXI	GAANNNNTTC	2 cut(s)	359, 2213
MscI	TGGCCA	2 cut(s)	20, 1455
MseI	TTAA	7 cut(s)	731, 1062, 1167, 1329, 1968, 2112, 2154
MslI	CAYNNNNRTG	1 cut(s)	2504
Msp20I	TGGCCA	2 cut(s)	20, 1455
MspA1I	CMGCKG	1 cut(s)	159
MspCI	CTTAAG	1 cut(s)	2153
MspI	CCGG	2 cut(s)	424, 1616
MspR9I	CCNGG	2 cut(s)	352, 496
MunI	CAATTG	2 cut(s)	1518, 2105
Mva1269I	GAATGC	1 cut(s)	119
MvaI	CCWGG	2 cut(s)	352, 496
MvnI	CGCG	3 cut(s)	231, 303, 522
MwoI	GCNNNNNNNGC	4 cut(s)	412, 496, 1865, 1949
NdeI	CATATG	1 cut(s)	1126
NdeII	GATC	7 cut(s)	480, 511, 690, 704, 772, 1238, 1340
NlaIV	GGNNCC	1 cut(s)	1342
NmuCI	GTSAC	2 cut(s)	305, 778
NsiI	ATGCAT	1 cut(s)	700
NspI	RCATGY	5 cut(s)	478, 646, 702, 1859, 1947
NspV	TTCGAA	1 cut(s)	534
PagI	TCATGA	1 cut(s)	2013
PciI	ACATGT	1 cut(s)	474
PcsI	WCGNNNNNNNCGW	1 cut(s)	144
PctI	GAATGC	1 cut(s)	119
PdmI	GAANNNNTTC	2 cut(s)	359, 2213
PfeI	GAWTC	6 cut(s)	1619, 1787, 2158, 2278, 2433, 2549
PflFI	GACNNNGTC	1 cut(s)	275
PleI	GAGTC	4 cut(s)	104, 577, 775, 2462
PpsI	GAGTC	4 cut(s)	104, 577, 775, 2462
Ppu21I	YACGTR	1 cut(s)	2486
PscI	ACATGT	1 cut(s)	474
PshBI	ATTAAT	1 cut(s)	1329
PsiI	TTATAA	2 cut(s)	471, 2423
Psp6I	CCWGG	2 cut(s)	350, 494
PspFI	CCCAGC	2 cut(s)	33, 618
PspGI	CCWGG	2 cut(s)	350, 494
PspN4I	GGNNCC	1 cut(s)	1342
PspPI	GGNCC	4 cut(s)	261, 621, 767, 1768
PstI	CTGCAG	2 cut(s)	1873, 2172
PstNI	CAGNNNCTG	1 cut(s)	2167
PsuI	RGATCY	2 cut(s)	704, 1340
PsyI	GACNNNGTC	1 cut(s)	275
RsaI	GTAC	3 cut(s)	1426, 2346, 2514
RsaNI	GTAC	3 cut(s)	1425, 2345, 2513
RseI	CAYNNNNRTG	1 cut(s)	2504
SalI	GTCGAC	1 cut(s)	146
SaqAI	TTAA	7 cut(s)	731, 1062, 1167, 1329, 1968, 2112, 2154
Sau3AI	GATC	7 cut(s)	480, 511, 690, 704, 772, 1238, 1340
Sau96I	GGNCC	4 cut(s)	261, 621, 767, 1768
SchI	GAGTC	4 cut(s)	104, 578, 775, 2462
ScrFI	CCNGG	2 cut(s)	352, 496
SduI	GDGCHC	7 cut(s)	327, 408, 457, 820, 1148, 1749, 2127
SfcI	CTRYAG	3 cut(s)	1869, 2168, 2574
SfuI	TTCGAA	1 cut(s)	534
SinI	GGWCC	1 cut(s)	767
SmiMI	CAYNNNNRTG	1 cut(s)	2504
SmlI	CTYRAG	2 cut(s)	440, 2153
SmoI	CTYRAG	2 cut(s)	440, 2153
SspI	AATATT	3 cut(s)	790, 824, 895
SspMI	CTAG	8 cut(s)	26, 666, 708, 911, 1260, 1989, 2136, 2340
StyD4I	CCNGG	2 cut(s)	350, 494
TaaI	ACNGT	4 cut(s)	410, 452, 927, 1673
TaiI	ACGT	3 cut(s)	141, 276, 2488
TaqI	TCGA	8 cut(s)	147, 234, 331, 534, 601, 2370, 2436, 2473
TaqII	GACCGA	2 cut(s)	122, 660
TauI	GCSGC	4 cut(s)	162, 192, 525, 1157
TfiI	GAWTC	6 cut(s)	1619, 1787, 2158, 2278, 2433, 2549
Tru1I	TTAA	7 cut(s)	731, 1062, 1167, 1329, 1968, 2112, 2154
Tru9I	TTAA	7 cut(s)	731, 1062, 1167, 1329, 1968, 2112, 2154
TscAI	CASTG	6 cut(s)	413, 466, 1329, 1351, 1678, 2330
TseFI	GTSAC	2 cut(s)	305, 778
TseI	GCWGC	8 cut(s)	116, 133, 646, 653, 1436, 1487, 1868, 2542
Tsp45I	GTSAC	2 cut(s)	305, 778
TspGWI	ACGGA	5 cut(s)	133, 254, 287, 860, 1793
TspRI	CASTG	6 cut(s)	413, 466, 1329, 1351, 1678, 2330
Tth111I	GACNNNGTC	1 cut(s)	275
Vha464I	CTTAAG	1 cut(s)	2153
VneI	GTGCAC	1 cut(s)	1144
VpaK11BI	GGWCC	1 cut(s)	767
VspI	ATTAAT	1 cut(s)	1329
XapI	RAATTY	5 cut(s)	72, 1398, 1552, 1713, 2028
XbaI	TCTAGA	1 cut(s)	1988
XceI	RCATGY	5 cut(s)	478, 646, 702, 1859, 1947
XcmI	CCANNNNNNNNNTGG	2 cut(s)	631, 2506
XmiI	GTMKAC	3 cut(s)	147, 1211, 1734
XmnI	GAANNNNTTC	2 cut(s)	359, 2213
XspI	CTAG	8 cut(s)	26, 666, 708, 911, 1260, 1989, 2136, 2340
ZraI	GACGTC	1 cut(s)	274
Zsp2I	ATGCAT	1 cut(s)	700

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.