Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0001022

Physical Location & Seq

Genomic Coordinates Forward (+)

16048287 .. 16052393

Gene Structure 4107 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1102600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1545 bp

ATGGCTGAGAGCGACGCCCCCGCCCCCGCCACAGCGACCGCAGAACCGTCGGCGCCGTCAGAAAAGTCGTCTCCCGAGGCGTCTACCACCGCTCCTCCGCCGGCGCAGAAGCCTGAAACAAAACGGTGGGCCGACGAAGAGGACGACCCTCCAGAGGAGCCCGAGAAGAAAGACGACGCGCCTGCGCCCTCTACTTCCGAGCCGGAGGTCAACCTTGCCGAATTGAAGATTACGGAGAACAAGTTCCTCGACGACCCCGAAGACTCTAATATCAGTGCCGTTACTGACGGAGACACGCCGTACACATCCGCGAGTAGTTTCGAGGATCTGAAACTGTCGCCGGAGGTACTGAAGGGTTTGTATGTAGAGATGCAATTCAAGAAGCCGAGCAAAATCCAGGCGATCACTTTGCCAATGATTTTGACCCCTCCCTATAAAGATCTTATTGCTCAGGCGCATAATGGTTCGGGCAAAACCACTTGTTTCGTGCTCGGGATGCTCAGCCGTGTCGATCCCAACCTCAAAGCTCCTCAGGCGCTCTGCATTTGCCCCACCAGAGAGTTGGCCATTCAGAATATGGAAGTTCTTCAGAAGATGGGGAAGTACACAGGGATAACTGCAGAGTGTGCTGTCCCTACAGATAGGACGAGTTCTACCTCGACGCAATCCAGGCCACCAGTGTCGGCACAAATTGTGATTGGAACTCCTGGTACAATTAAGAGATTAATGTCGACGAAAAAACTCGGTGTGAGCAAGGTCAAGGTTCTTGTTTTCGACGAGGCTGATCACATGCTTGCCGAGGATGGCTTTAAGGATGATTCTTTGAGGATAATGAAGGACATAGAGAGGTTTAGCTCTCACTGCCAGGTGCTTTTATTTTCTGCTACATTTAATGAAAAAGTCACAAACTTTGTTTCAAAAGTGATCAAAGGTGGTGGTAATCAACTCTTTGTAAAGAAAGAAGAGCTATCTTTGGAGGCTGTGAAGCAGTACAAGGTGTACTGCCCTGATGAATTGGCAAAGATCGACGTCATCAAAAGTAAAATATTTGAACTGGGAGAGAAATTGGGGCAGAGAATTATATTTGTACGGACAAAAAATAGCGCAAGAATGTTGCATCAGCAACTTGTTCAAGATGGTTATGCAGTTACTACTATTCAAGGTGCCCTCAATGCTGAAGATAGAGACAAAATAGTCAAAGAGTTCAAAGATGGCTTAACCCAAGTTCTTATATCAACTGACCTACTCGCTCGAGGCTTTGATCAACAGCAGGTTAATTGTGTTGTCAATTATGATCTTCCAATCAAATATAGAGAGGGCCCCAGGACACGTGATCCTCCCGAACCTGATTATGAGGTCTACTTGCATAGAATTGGAAGGGCAGGACGTTTTGGTCGCAAGGGGGCTGTGTTTAACTTGATTTGCGGTGATTGGGATGAATTGACCATGTCAAAGATTGAGCAGTATTTTAACACCCCAGTAACAGAGGTTCAAAACACCGAAGAAGCTTTTGAAGGCGCTCTTAAAGCTGCCGGTTTACTGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005635 GO:0005730 GO:0005737 GO:0005886 GO:0006139 GO:0006403 GO:0006405 GO:0006406 GO:0006611 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0006913 GO:0006950 GO:0008026 GO:0008104 GO:0008150 GO:0008152 GO:0008186 GO:0009266 GO:0009408 GO:0009409 GO:0009628 GO:0009719 GO:0009725 GO:0009737 GO:0009987 GO:0010033 GO:0010467 GO:0010501 GO:0012505 GO:0015031 GO:0015833 GO:0015931 GO:0016020 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0016973 GO:0017111 GO:0031503 GO:0031967 GO:0031974 GO:0031975 GO:0031981 GO:0033036 GO:0033993 GO:0034613 GO:0034641 GO:0042221 GO:0042623 GO:0042886 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045184 GO:0046483 GO:0046907 GO:0050657 GO:0050658 GO:0050896 GO:0051028 GO:0051168 GO:0051169 GO:0051179 GO:0051234 GO:0051236 GO:0051641 GO:0051649 GO:0070013 GO:0070035 GO:0070727 GO:0071166 GO:0071426 GO:0071427 GO:0071702 GO:0071704 GO:0071705 GO:0071944 GO:0090304 GO:0097305 GO:0140098 GO:1901360 GO:1901700

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DEAD Helicase_C

Protein Analysis

515

Amino Acids

56.74

Weight (kDa)

5.19

Isoelectric Point (pI)

37.44

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DEAD	PF00270	131 - 303	2.6e-38	DEAD/DEAH box helicase
ResIII	PF04851	147 - 298	1.7e-09	Type III restriction enzyme, res subunit
Helicase_C	PF00271	348 - 465	4.2e-24	Helicase conserved C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001022)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G53110
fragaria_vesca	FvH4_6g17230 FvH4_6g17230 FvH4_6g17230 FvH4_6g17280 FvH4_6g17280
malus_domestica	MD12G1102600.v1.1 MD14G1096800.v1.1
prunus_persica	Prupe.3G009100_v2.0.a1 Prupe.7G122600_v2.0.a1
pyrus_communis	pycom03g08760 pycom12g10060 pycom14g09450
rosa_chinensis	RchiOBHm_Chr3g0471311 RchiOBHm_Chr3g0471321 RchiOBHm_Chr3g0471361 RchiOBHm_Chr3g0471391
rosa_laevigata	RLG00000024181 RLG00000024187 RLG00000024188
rosa_multiflora	Rmu_sc0006582.1_g000015 Rmu_sc0006582.1_g000018 Rmu_sc0009082.1_g000003 Rmu_sc0010513.1_g000003 Rmu_sc0015755.1_g000002 Rmu_sc0030425.1_g000001
rosa_roxburghii	Rroxscaffold_6G00409980 Rroxscaffold_6G00410000 Rroxscaffold_6G00410060 Rroxscaffold_6G00410080
rosa_rugosa	Rorug03G0117100 Rorug03G0117100 Rorug03G0117100 Rorug03G0117400 Rorug03G0117500 Rorug03G0117500
rosa_samantha	Rh3AG166500 Rh3AG166600 Rh3AG166700 Rh3AG167100 Rh3BG191900 Rh3BG192000 Rh3BG192100 Rh3BG192400 Rh3BG192500 Rh3BG192800 Rh3CG182200 Rh3CG182300 Rh3CG182400 Rh3CG182700 Rh3CG182800 Rh3CG183200 Rh3DG182700 Rh3DG183100 Rh3DG183200 Rh3DG183700 Rh3DG183800
rosa_wichuraiana	Rw3G015670 Rw3G015680 Rw3G015730 Rw3G015760

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	1193, 1392
AatII	GACGTC	1 cut(s)	1032
Acc36I	ACCTGC	1 cut(s)	1261
AccB1I	GGYRCC	2 cut(s)	52, 1163
AccBSI	CCGCTC	1 cut(s)	92
AccI	GTMKAC	3 cut(s)	83, 731, 1359
AccII	CGCG	2 cut(s)	179, 311
AciI	CCGC	7 cut(s)	21, 27, 39, 90, 98, 309, 1425
AclWI	GGATC	3 cut(s)	333, 506, 1328
AcoI	YGGCCR	1 cut(s)	564
AcuI	CTGAAG	3 cut(s)	371, 572, 1197
AcvI	CACGTG	1 cut(s)	1331
AcyI	GRCGYC	4 cut(s)	15, 53, 80, 1029
AfaI	GTAC	7 cut(s)	302, 348, 605, 712, 992, 1001, 1089
AfiI	CCNNNNNNNGG	1 cut(s)	154
AflIII	ACRYGT	1 cut(s)	1328
AgsI	TTSAA	9 cut(s)	226, 379, 918, 1052, 1133, 1160, 1207, 1493, 1514
AjnI	CCWGG	5 cut(s)	396, 668, 706, 864, 1322
AloI	GAACNNNNNNTCC	2 cut(s)	227, 259
AluBI	AGCT	5 cut(s)	527, 855, 967, 1508, 1529
AluI	AGCT	5 cut(s)	527, 855, 967, 1508, 1529
Alw21I	GWGCWC	1 cut(s)	492
Alw26I	GTCTC	3 cut(s)	75, 285, 1179
AlwI	GGATC	3 cut(s)	333, 506, 1328
Ama87I	CYCGRG	4 cut(s)	74, 161, 491, 1251
AoxI	GGCC	4 cut(s)	129, 564, 671, 1318
ApaI	GGGCCC	1 cut(s)	1322
ApeKI	GCWGC	1 cut(s)	1529
AseI	ATTAAT	1 cut(s)	725
Asp700I	GAANNNNTTC	2 cut(s)	242, 585
AspLEI	GCGC	8 cut(s)	55, 106, 181, 187, 457, 538, 1106, 1520
AspS9I	GGNCC	3 cut(s)	129, 1318, 1319
AsuHPI	GGTGA	1 cut(s)	1439
AvaI	CYCGRG	4 cut(s)	74, 161, 491, 1251
AxyI	CCTNAGG	1 cut(s)	531
BaeGI	GKGCMC	2 cut(s)	1168, 1322
BalI	TGGCCA	1 cut(s)	566
BanI	GGYRCC	2 cut(s)	52, 1163
BanII	GRGCYC	2 cut(s)	162, 1322
BbrPI	CACGTG	1 cut(s)	1331
BbsI	GAAGAC	1 cut(s)	267
Bbv12I	GWGCWC	1 cut(s)	492
BbvI	GCAGC	1 cut(s)	1516
BccI	CCATC	4 cut(s)	589, 797, 1130, 1205
BceAI	ACGGC	4 cut(s)	40, 263, 283, 489
BcgI	CGANNNNNNTGC	6 cut(s)	30, 64, 164, 198, 391, 425
BciT130I	CCWGG	5 cut(s)	398, 670, 708, 866, 1324
BclI	TGATCA	3 cut(s)	784, 924, 1261
BcoDI	GTCTC	3 cut(s)	75, 285, 1179
BfmI	CTRYAG	2 cut(s)	618, 636
BfoI	RGCGCY	3 cut(s)	56, 539, 1521
BfuAI	ACCTGC	1 cut(s)	1261
BglII	AGATCT	1 cut(s)	439
BisI	GCNGC	1 cut(s)	1530
BlpI	GCTNAGC	1 cut(s)	500
BlsI	GCNGC	1 cut(s)	1531
Bme1390I	CCNGG	5 cut(s)	398, 670, 708, 866, 1324
BmeT110I	CYCGRG	4 cut(s)	74, 161, 491, 1251
BmgT120I	GGNCC	3 cut(s)	129, 1318, 1319
BmiI	GGNNCC	5 cut(s)	54, 159, 1165, 1320, 1321
BmrFI	CCNGG	5 cut(s)	398, 670, 708, 866, 1324
BmrI	ACTGGG	2 cut(s)	1064, 1472
BmsI	GCATC	3 cut(s)	360, 486, 1126
BmuI	ACTGGG	2 cut(s)	1064, 1472
BpiI	GAAGAC	1 cut(s)	267
BpmI	CTGGAG	1 cut(s)	135
Bpu10I	CCTNAGC	1 cut(s)	450
Bpu1102I	GCTNAGC	1 cut(s)	500
BsaAI	YACGTR	1 cut(s)	1331
BsaHI	GRCGYC	4 cut(s)	15, 53, 80, 1029
BsaJI	CCNNGG	3 cut(s)	75, 798, 1322
BsaXI	ACNNNNNCTCC	6 cut(s)	76, 79, 106, 109, 227, 257
Bsc4I	CCNNNNNNNGG	1 cut(s)	154
Bse118I	RCCGGY	2 cut(s)	100, 1532
Bse1I	ACTGG	3 cut(s)	677, 1059, 1478
Bse21I	CCTNAGG	1 cut(s)	531
BseBI	CCWGG	5 cut(s)	398, 670, 708, 866, 1324
BseDI	CCNNGG	3 cut(s)	75, 798, 1322
BseGI	GGATG	5 cut(s)	305, 501, 808, 820, 1441
BseLI	CCNNNNNNNGG	1 cut(s)	154
BseMII	CTCAG	3 cut(s)	464, 514, 545
BseNI	ACTGG	3 cut(s)	677, 1059, 1478
BseRI	GAGGAG	3 cut(s)	84, 170, 519
BseSI	GKGCMC	2 cut(s)	1168, 1322
BseXI	GCAGC	1 cut(s)	1516
Bsh1236I	CGCG	2 cut(s)	179, 311
Bsh1285I	CGRYCG	1 cut(s)	39
BshFI	GGCC	4 cut(s)	131, 566, 673, 1320
BshNI	GGYRCC	2 cut(s)	52, 1163
BsiEI	CGRYCG	1 cut(s)	39
BsiHKAI	GWGCWC	1 cut(s)	492
BsiHKCI	CYCGRG	4 cut(s)	74, 161, 491, 1251
BsiSI	CCGG	4 cut(s)	101, 203, 341, 1533
BslFI	GGGAC	1 cut(s)	617
BslI	CCNNNNNNNGG	1 cut(s)	154
BsmAI	GTCTC	3 cut(s)	75, 285, 1179
BsmBI	CGTCTC	1 cut(s)	75
BsmFI	GGGAC	1 cut(s)	617
BsnI	GGCC	4 cut(s)	131, 566, 673, 1320
BsoBI	CYCGRG	4 cut(s)	74, 161, 491, 1251
Bsp120I	GGGCCC	1 cut(s)	1318
Bsp1286I	GDGCHC	4 cut(s)	162, 492, 1168, 1322
Bsp1720I	GCTNAGC	1 cut(s)	500
BspACI	CCGC	7 cut(s)	21, 27, 39, 90, 98, 309, 1425
BspANI	GGCC	4 cut(s)	131, 566, 673, 1320
BspCNI	CTCAG	3 cut(s)	463, 513, 544
BspFNI	CGCG	2 cut(s)	179, 311
BspLI	GGNNCC	5 cut(s)	54, 159, 1165, 1320, 1321
BspMAI	CTGCAG	1 cut(s)	622
BspMI	ACCTGC	1 cut(s)	1261
BspPI	GGATC	3 cut(s)	333, 506, 1328
BspQI	GCTCTTC	1 cut(s)	957
BspT107I	GGYRCC	2 cut(s)	52, 1163
BsrBI	CCGCTC	1 cut(s)	92
BsrFI	RCCGGY	2 cut(s)	100, 1532
BsrI	ACTGG	3 cut(s)	677, 1059, 1478
BssAI	RCCGGY	2 cut(s)	100, 1532
BssECI	CCNNGG	3 cut(s)	75, 798, 1322
BssNI	GRCGYC	4 cut(s)	15, 53, 80, 1029
Bst2UI	CCWGG	5 cut(s)	398, 670, 708, 866, 1324
Bst4CI	ACNGT	4 cut(s)	48, 126, 336, 1542
Bst6I	CTCTTC	2 cut(s)	132, 957
BstACI	GRCGYC	4 cut(s)	15, 53, 80, 1029
BstAPI	GCANNNNNTGC	1 cut(s)	626
BstBAI	YACGTR	1 cut(s)	1331
BstC8I	GCNNGC	3 cut(s)	102, 183, 795
BstDEI	CTNAG	4 cut(s)	6, 450, 500, 531
BstF5I	GGATG	5 cut(s)	305, 501, 808, 820, 1441
BstFNI	CGCG	2 cut(s)	179, 311
BstH2I	RGCGCY	3 cut(s)	56, 539, 1521
BstHHI	GCGC	8 cut(s)	55, 106, 181, 187, 457, 538, 1106, 1520
BstMAI	GTCTC	3 cut(s)	75, 285, 1179
BstMCI	CGRYCG	1 cut(s)	39
BstMWI	GCNNNNNNNGC	7 cut(s)	496, 533, 626, 670, 861, 1172, 1526
BstNI	CCWGG	5 cut(s)	398, 670, 708, 866, 1324
BstNSI	RCATGY	1 cut(s)	793
BstSCI	CCNGG	5 cut(s)	396, 668, 706, 864, 1322
BstSFI	CTRYAG	2 cut(s)	618, 636
BstSLI	GKGCMC	2 cut(s)	1168, 1322
BstUI	CGCG	2 cut(s)	179, 311
BstV1I	GCAGC	1 cut(s)	1516
BstV2I	GAAGAC	1 cut(s)	267
BstX2I	RGATCY	2 cut(s)	325, 439
BstXI	CCANNNNNNTGG	1 cut(s)	562
BstYI	RGATCY	2 cut(s)	325, 439
Bsu36I	CCTNAGG	1 cut(s)	531
BsuRI	GGCC	4 cut(s)	131, 566, 673, 1320
BtsCI	GGATG	5 cut(s)	305, 501, 808, 820, 1441
BtsI	GCAGTG	1 cut(s)	859
BtsIMutI	CAGTG	3 cut(s)	280, 684, 859
BveI	ACCTGC	1 cut(s)	1261
Cac8I	GCNNGC	3 cut(s)	102, 183, 795
CfoI	GCGC	8 cut(s)	55, 106, 181, 187, 457, 538, 1106, 1520
Cfr10I	RCCGGY	2 cut(s)	100, 1532
Cfr13I	GGNCC	3 cut(s)	129, 1318, 1319
CseI	GACGC	4 cut(s)	23, 69, 185, 670
Csp6I	GTAC	7 cut(s)	301, 347, 604, 711, 991, 1000, 1088
CspCI	CAANNNNNGTGG	2 cut(s)	916, 951
CviAII	CATG	2 cut(s)	790, 1447
CviQI	GTAC	7 cut(s)	301, 347, 604, 711, 991, 1000, 1088
DdeI	CTNAG	4 cut(s)	6, 450, 500, 531
DinI	GGCGCC	1 cut(s)	54
DrdI	GACNNNNNNGTC	2 cut(s)	1193, 1392
DseDI	GACNNNNNNGTC	2 cut(s)	1193, 1392
EaeI	YGGCCR	1 cut(s)	564
Eam1104I	CTCTTC	2 cut(s)	132, 957
EarI	CTCTTC	2 cut(s)	132, 957
EciI	GGCGGA	1 cut(s)	87
Eco24I	GRGCYC	2 cut(s)	162, 1322
Eco57I	CTGAAG	3 cut(s)	371, 572, 1197
Eco72I	CACGTG	1 cut(s)	1331
Eco81I	CCTNAGG	1 cut(s)	531
Eco88I	CYCGRG	4 cut(s)	74, 161, 491, 1251
EcoO109I	RGGNCCY	2 cut(s)	1318, 1319
EcoRII	CCWGG	5 cut(s)	396, 668, 706, 864, 1322
EcoT38I	GRGCYC	2 cut(s)	162, 1322
EgeI	GGCGCC	1 cut(s)	54
EheI	GGCGCC	1 cut(s)	54
Esp3I	CGTCTC	1 cut(s)	75
FaeI	CATG	2 cut(s)	793, 1450
FalI	AAGNNNNNCTT	2 cut(s)	1506, 1538
FaqI	GGGAC	1 cut(s)	617
FatI	CATG	2 cut(s)	789, 1446
FauI	CCCGC	2 cut(s)	28, 34
FbaI	TGATCA	3 cut(s)	784, 924, 1261
FblI	GTMKAC	3 cut(s)	83, 731, 1359
Fnu4HI	GCNGC	1 cut(s)	1530
FokI	GGATG	5 cut(s)	292, 508, 815, 827, 1448
FriOI	GRGCYC	2 cut(s)	162, 1322
Fsp4HI	GCNGC	1 cut(s)	1530
GlaI	GCGC	8 cut(s)	54, 105, 180, 186, 456, 537, 1105, 1519
GluI	GCNGC	1 cut(s)	1530
GsuI	CTGGAG	1 cut(s)	135
HaeII	RGCGCY	3 cut(s)	56, 539, 1521
HaeIII	GGCC	4 cut(s)	131, 566, 673, 1320
HapII	CCGG	4 cut(s)	101, 203, 341, 1533
HgaI	GACGC	4 cut(s)	23, 69, 185, 670
HhaI	GCGC	8 cut(s)	55, 106, 181, 187, 457, 538, 1106, 1520
Hin1I	GRCGYC	4 cut(s)	15, 53, 80, 1029
Hin1II	CATG	2 cut(s)	793, 1450
Hin6I	GCGC	8 cut(s)	53, 104, 179, 185, 455, 536, 1104, 1518
HinP1I	GCGC	8 cut(s)	53, 104, 179, 185, 455, 536, 1104, 1518
HincII	GTYRAC	2 cut(s)	211, 732
HindII	GTYRAC	2 cut(s)	211, 732
HindIII	AAGCTT	1 cut(s)	1506
HinfI	GANTC	2 cut(s)	263, 818
HpaII	CCGG	4 cut(s)	101, 203, 341, 1533
HphI	GGTGA	1 cut(s)	1439
Hpy166II	GTNNAC	8 cut(s)	84, 211, 303, 606, 732, 1000, 1360, 1538
Hpy188I	TCNGA	5 cut(s)	61, 199, 330, 573, 591
Hpy188III	TCNNGA	6 cut(s)	74, 152, 379, 493, 1133, 1340
Hpy8I	GTNNAC	8 cut(s)	84, 211, 303, 606, 732, 1000, 1360, 1538
Hpy99I	CGWCG	9 cut(s)	17, 52, 137, 179, 254, 664, 736, 779, 1031
HpyAV	CCTTC	4 cut(s)	346, 829, 1371, 1508
HpyCH4III	ACNGT	4 cut(s)	48, 126, 336, 1542
HpyCH4IV	ACGT	3 cut(s)	1029, 1330, 1387
HpyCH4V	TGCA	6 cut(s)	373, 543, 620, 1117, 1145, 1366
HpyF10VI	GCNNNNNNNGC	7 cut(s)	496, 533, 626, 670, 861, 1172, 1526
HpyF3I	CTNAG	4 cut(s)	6, 450, 500, 531
HpySE526I	ACGT	3 cut(s)	1029, 1330, 1387
Hsp92I	GRCGYC	4 cut(s)	15, 53, 80, 1029
Hsp92II	CATG	2 cut(s)	793, 1450
HspAI	GCGC	8 cut(s)	53, 104, 179, 185, 455, 536, 1104, 1518
KasI	GGCGCC	1 cut(s)	52
KroI	GCCGGC	1 cut(s)	100
KroNI	GCCGGC	1 cut(s)	102
Ksp22I	TGATCA	3 cut(s)	784, 924, 1261
LguI	GCTCTTC	1 cut(s)	957
LmnI	GCTCC	3 cut(s)	97, 157, 532
Lsp1109I	GCAGC	1 cut(s)	1516
LweI	GCATC	3 cut(s)	360, 486, 1126
MaeII	ACGT	3 cut(s)	1029, 1330, 1387
MaeIII	GTNAC	4 cut(s)	280, 901, 1147, 1480
MbiI	CCGCTC	1 cut(s)	92
MflI	RGATCY	2 cut(s)	325, 439
MhlI	GDGCHC	4 cut(s)	162, 492, 1168, 1322
MlsI	TGGCCA	1 cut(s)	566
MluNI	TGGCCA	1 cut(s)	566
Mly113I	GGCGCC	1 cut(s)	53
MlyI	GAGTC	1 cut(s)	257
Mox20I	TGGCCA	1 cut(s)	566
MreI	CGCCGGCG	1 cut(s)	100
MroNI	GCCGGC	1 cut(s)	100
MroXI	GAANNNNTTC	2 cut(s)	242, 585
MscI	TGGCCA	1 cut(s)	566
MseI	TTAA	9 cut(s)	717, 725, 810, 891, 1217, 1275, 1413, 1470, 1524
Msp20I	TGGCCA	1 cut(s)	566
MspI	CCGG	4 cut(s)	101, 203, 341, 1533
MspR9I	CCNGG	5 cut(s)	398, 670, 708, 866, 1324
MvaI	CCWGG	5 cut(s)	398, 670, 708, 866, 1324
MvnI	CGCG	2 cut(s)	179, 311
MwoI	GCNNNNNNNGC	7 cut(s)	496, 533, 626, 670, 861, 1172, 1526
NaeI	GCCGGC	1 cut(s)	102
NarI	GGCGCC	1 cut(s)	53
NgoMIV	GCCGGC	1 cut(s)	100
NlaIII	CATG	2 cut(s)	793, 1450
NlaIV	GGNNCC	5 cut(s)	54, 159, 1165, 1320, 1321
NmeAIII	GCCGAG	2 cut(s)	411, 823
NmuCI	GTSAC	1 cut(s)	901
NspI	RCATGY	1 cut(s)	793
PaeR7I	CTCGAG	1 cut(s)	1251
PciSI	GCTCTTC	1 cut(s)	957
PcsI	WCGNNNNNNNCGW	2 cut(s)	141, 255
PdiI	GCCGGC	1 cut(s)	102
PdmI	GAANNNNTTC	2 cut(s)	242, 585
PfeI	GAWTC	1 cut(s)	818
PflFI	GACNNNGTC	1 cut(s)	1447
PkrI	GCNGC	1 cut(s)	1531
PleI	GAGTC	1 cut(s)	257
PluTI	GGCGCC	1 cut(s)	56
PmaCI	CACGTG	1 cut(s)	1331
PmlI	CACGTG	1 cut(s)	1331
PpsI	GAGTC	1 cut(s)	257
Ppu21I	YACGTR	1 cut(s)	1331
PshBI	ATTAAT	1 cut(s)	725
Psp6I	CCWGG	5 cut(s)	396, 668, 706, 864, 1322
PspCI	CACGTG	1 cut(s)	1331
PspGI	CCWGG	5 cut(s)	396, 668, 706, 864, 1322
PspN4I	GGNNCC	5 cut(s)	54, 159, 1165, 1320, 1321
PspOMI	GGGCCC	1 cut(s)	1318
PspPI	GGNCC	3 cut(s)	129, 1318, 1319
PspXI	VCTCGAGB	1 cut(s)	1251
PsrI	GAACNNNNNNTAC	4 cut(s)	694, 726, 1473, 1505
PstI	CTGCAG	1 cut(s)	622
PsuI	RGATCY	2 cut(s)	325, 439
PsyI	GACNNNGTC	1 cut(s)	1447
RsaI	GTAC	7 cut(s)	302, 348, 605, 712, 992, 1001, 1089
RsaNI	GTAC	7 cut(s)	301, 347, 604, 711, 991, 1000, 1088
SalI	GTCGAC	1 cut(s)	730
SapI	GCTCTTC	1 cut(s)	957
SaqAI	TTAA	9 cut(s)	717, 725, 810, 891, 1217, 1275, 1413, 1470, 1524
SatI	GCNGC	1 cut(s)	1530
Sau96I	GGNCC	3 cut(s)	129, 1318, 1319
SchI	GAGTC	1 cut(s)	257
ScrFI	CCNGG	5 cut(s)	398, 670, 708, 866, 1324
SduI	GDGCHC	4 cut(s)	162, 492, 1168, 1322
SfaNI	GCATC	3 cut(s)	360, 486, 1126
SfcI	CTRYAG	2 cut(s)	618, 636
SfoI	GGCGCC	1 cut(s)	54
Sfr274I	CTCGAG	1 cut(s)	1251
SgrAI	CRCCGGYG	1 cut(s)	100
SlaI	CTCGAG	1 cut(s)	1251
SmlI	CTYRAG	1 cut(s)	1251
SmoI	CTYRAG	1 cut(s)	1251
SsiI	CCGC	7 cut(s)	21, 27, 39, 90, 98, 309, 1425
SspDI	GGCGCC	1 cut(s)	52
SspI	AATATT	1 cut(s)	1047
StyD4I	CCNGG	5 cut(s)	396, 668, 706, 864, 1322
TaaI	ACNGT	4 cut(s)	48, 126, 336, 1542
TaiI	ACGT	3 cut(s)	1032, 1333, 1390
TaqI	TCGA	8 cut(s)	249, 321, 510, 659, 731, 774, 1026, 1252
TatI	WGTACW	3 cut(s)	603, 990, 999
TfiI	GAWTC	1 cut(s)	818
Tru1I	TTAA	9 cut(s)	717, 725, 810, 891, 1217, 1275, 1413, 1470, 1524
Tru9I	TTAA	9 cut(s)	717, 725, 810, 891, 1217, 1275, 1413, 1470, 1524
TscAI	CASTG	3 cut(s)	280, 684, 866
TseFI	GTSAC	1 cut(s)	901
TseI	GCWGC	1 cut(s)	1529
Tsp45I	GTSAC	1 cut(s)	901
TspDTI	ATGAA	4 cut(s)	848, 909, 1026, 1452
TspGWI	ACGGA	3 cut(s)	248, 303, 1105
TspRI	CASTG	3 cut(s)	280, 684, 866
Tth111I	GACNNNGTC	1 cut(s)	1447
VspI	ATTAAT	1 cut(s)	725
XceI	RCATGY	1 cut(s)	793
XcmI	CCANNNNNNNNNTGG	2 cut(s)	559, 574
XhoI	CTCGAG	1 cut(s)	1251
XmiI	GTMKAC	3 cut(s)	83, 731, 1359
XmnI	GAANNNNTTC	2 cut(s)	242, 585
ZraI	GACGTC	1 cut(s)	1030

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.