Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0001022

Physical Location & Seq

Genomic Coordinates Forward (+)

14695116 .. 14699383

Gene Structure 4268 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1096800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1557 bp

ATGGCTGAGTCTGCCGCCCCCGCCGCTGTCACATCAACCGTAGAACCCGCAGCGCCATCAGGAAACAAGTCGTCTCCCGAGGCTTCTACGACCACTCCTCCGCCGGCGGAGAAGCCTGAGAAGTCGGAGACAAAACGATGGGCCGACGAAGAGGACGACCCTCCGGAGGAGCCCGAGAAGAAAGACGACGCTCCGGCGCCGTCTACTTCGGAACCGGAGGTAAACCTTGCCGAATTGAAGATTACCGAGAACAAGTTCCTCGACGACCCCGAAGACTCTAAAATCAGTGCCGTTACGGCCGGTGACACGCCGTACACGTCGGCGAGTAGTTTCGAGGATCTGAATCTGTCGCCAGATGTACTGAAGGGCTTGTATGTCGAGATGCAATTCAAAAAGCCGAGCAAAATCCAGGCAATCACTTTGCCAATGATTTTGACCCCTCCCTACAAAGATCTTATTGCTCAGGCGCATAATGGTTCGGGCAAAACCACTTGTTTCGTGCTTGGGATGCTCAGCCGTGTCGATCCCAATATCAAAGCTCCTCAGGCGCTCTGCATTTGCCCCACCAGAGAGTTGGCCATTCAGAATCTCGAGGTTCTTCAGAAGATGGGGAAGTATACAGGGATAACTGCAGAGTGTGCTATCCCAACAGATAGGACAAGTTCTACCTCGGCACAATCCAGGCCACCAGTATCGGCACAAATTGTGATTGGAACTCCTGGTACGATTAAGAGATTAATGTCGACGAAAAAAATCGGTGTGAGCTGTGTCAAGATTCTTGTTTTCGACGAGGCTGATCACATGCTTGCTGAGGATGGCTTTAAGGATGATTCTTTGAGGATAATGAATGACATAAAGAGATATAGCTCTCACTGCCAGGTGCTTTTGTTTTCTGCTACATTTAATGAAAAAGTCACGAACTTTGTTTCAAAAGTGATCAAAGGTGGTGGTAACCAACTCTTTGTAAAGAAAGAAGAGCTATCATTGGAGGCTGTAAAGCAGTACAAGGTGTACTGTCCTGATGAATTGGCAAAGATTGACGTCATCAAAAGCAAAATATTTGAACTGGGAGAGAAATTGGGGCAGAGAATTATATTTGTTCGGACAAAAAATAGTGCAAGAATGTTACATCAGCAACTTGTACAAGATGGTTATGCAGTTACTACTATTCAAGGTGCCCTCAATGCTGAAGATAGAGACAAAATAGTCAAAGAGTTCAAAGATGGTTTAACCCAAGTTCTTATATCAACTGACCTACTCGCTCGAGGATTTGATCAACAGCAGATTAATTGTGTTGTCAATTATGACCTTCCAATCAAATATAGAGAGGGCCCCAGGACACGTGATCCTCCCGAACCTGATTATGAGGTGTACTTGCATAGAATTGGAAGGGCTGGACGTTTTGGTCGCAAGGGGGCGGTGTTTAACTTGATTTGCGGTGATTGGGATGAATTGACCATGTCAAAGATTGAGCAGTATTTTAACTCTCCAGTAACAGAGGTTGAAAACACCGAAGAAGCTTTCGAAGGCGCTCTGAAAGCAGCTGGCTTACTGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005635 GO:0005730 GO:0005737 GO:0005886 GO:0006139 GO:0006403 GO:0006405 GO:0006406 GO:0006611 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0006913 GO:0006950 GO:0008026 GO:0008104 GO:0008150 GO:0008152 GO:0008186 GO:0009266 GO:0009408 GO:0009409 GO:0009628 GO:0009719 GO:0009725 GO:0009737 GO:0009987 GO:0010033 GO:0010467 GO:0010501 GO:0012505 GO:0015031 GO:0015833 GO:0015931 GO:0016020 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0016973 GO:0017111 GO:0031503 GO:0031967 GO:0031974 GO:0031975 GO:0031981 GO:0033036 GO:0033993 GO:0034613 GO:0034641 GO:0042221 GO:0042623 GO:0042886 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045184 GO:0046483 GO:0046907 GO:0050657 GO:0050658 GO:0050896 GO:0051028 GO:0051168 GO:0051169 GO:0051179 GO:0051234 GO:0051236 GO:0051641 GO:0051649 GO:0070013 GO:0070035 GO:0070727 GO:0071166 GO:0071426 GO:0071427 GO:0071702 GO:0071704 GO:0071705 GO:0071944 GO:0090304 GO:0097305 GO:0140098 GO:1901360 GO:1901700

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DEAD Helicase_C

Protein Analysis

519

Amino Acids

57.04

Weight (kDa)

5.25

Isoelectric Point (pI)

37.29

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DEAD	PF00270	135 - 307	4.5e-37	DEAD/DEAH box helicase
ResIII	PF04851	151 - 302	3.3e-08	Type III restriction enzyme, res subunit
Helicase_C	PF00271	352 - 469	7.2e-24	Helicase conserved C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001022)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G53110
fragaria_vesca	FvH4_6g17230 FvH4_6g17230 FvH4_6g17230 FvH4_6g17280 FvH4_6g17280
malus_domestica	MD12G1102600.v1.1 MD14G1096800.v1.1
prunus_persica	Prupe.3G009100_v2.0.a1 Prupe.7G122600_v2.0.a1
pyrus_communis	pycom03g08760 pycom12g10060 pycom14g09450
rosa_chinensis	RchiOBHm_Chr3g0471311 RchiOBHm_Chr3g0471321 RchiOBHm_Chr3g0471361 RchiOBHm_Chr3g0471391
rosa_laevigata	RLG00000024181 RLG00000024187 RLG00000024188
rosa_multiflora	Rmu_sc0006582.1_g000015 Rmu_sc0006582.1_g000018 Rmu_sc0009082.1_g000003 Rmu_sc0010513.1_g000003 Rmu_sc0015755.1_g000002 Rmu_sc0030425.1_g000001
rosa_roxburghii	Rroxscaffold_6G00409980 Rroxscaffold_6G00410000 Rroxscaffold_6G00410060 Rroxscaffold_6G00410080
rosa_rugosa	Rorug03G0117100 Rorug03G0117100 Rorug03G0117100 Rorug03G0117400 Rorug03G0117500 Rorug03G0117500
rosa_samantha	Rh3AG166500 Rh3AG166600 Rh3AG166700 Rh3AG167100 Rh3BG191900 Rh3BG192000 Rh3BG192100 Rh3BG192400 Rh3BG192500 Rh3BG192800 Rh3CG182200 Rh3CG182300 Rh3CG182400 Rh3CG182700 Rh3CG182800 Rh3CG183200 Rh3DG182700 Rh3DG183100 Rh3DG183200 Rh3DG183700 Rh3DG183800
rosa_wichuraiana	Rw3G015670 Rw3G015680 Rw3G015730 Rw3G015760

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	1205, 1404
AatII	GACGTC	1 cut(s)	1044
AccB1I	GGYRCC	2 cut(s)	196, 1175
AccI	GTMKAC	3 cut(s)	203, 617, 743
AccIII	TCCGGA	1 cut(s)	163
AciI	CCGC	8 cut(s)	15, 21, 24, 48, 101, 107, 1418, 1437
AclWI	GGATC	3 cut(s)	345, 518, 1340
AcoI	YGGCCR	2 cut(s)	297, 576
AcuI	CTGAAG	3 cut(s)	383, 584, 1209
AcvI	CACGTG	1 cut(s)	1343
AcyI	GRCGYC	2 cut(s)	197, 1041
AfaI	GTAC	7 cut(s)	314, 360, 724, 1004, 1013, 1143, 1373
AfiI	CCNNNNNNNGG	1 cut(s)	166
AflIII	ACRYGT	2 cut(s)	315, 1340
AgsI	TTSAA	7 cut(s)	238, 391, 930, 1064, 1172, 1219, 1505
AjiI	CACGTC	1 cut(s)	318
AjnI	CCWGG	5 cut(s)	408, 680, 718, 876, 1334
AluBI	AGCT	6 cut(s)	539, 765, 867, 979, 1520, 1544
AluI	AGCT	6 cut(s)	539, 765, 867, 979, 1520, 1544
Alw26I	GTCTC	3 cut(s)	78, 122, 1191
AlwI	GGATC	3 cut(s)	345, 518, 1340
Ama87I	CYCGRG	4 cut(s)	77, 173, 590, 1263
Aor13HI	TCCGGA	1 cut(s)	163
AoxI	GGCC	5 cut(s)	141, 297, 576, 683, 1330
ApaI	GGGCCC	1 cut(s)	1334
ApeKI	GCWGC	2 cut(s)	50, 1541
AseI	ATTAAT	2 cut(s)	737, 1287
Asp700I	GAANNNNTTC	1 cut(s)	254
AspLEI	GCGC	5 cut(s)	55, 199, 469, 550, 1532
AspS9I	GGNCC	3 cut(s)	141, 1330, 1331
AsuHPI	GGTGA	2 cut(s)	314, 1451
AsuII	TTCGAA	1 cut(s)	1524
AvaI	CYCGRG	4 cut(s)	77, 173, 590, 1263
AxyI	CCTNAGG	1 cut(s)	543
BaeGI	GKGCMC	2 cut(s)	1180, 1334
BalI	TGGCCA	1 cut(s)	578
BanI	GGYRCC	2 cut(s)	196, 1175
BanII	GRGCYC	2 cut(s)	174, 1334
BbrPI	CACGTG	1 cut(s)	1343
BbsI	GAAGAC	1 cut(s)	279
BbvCI	CCTCAGC	1 cut(s)	810
BbvI	GCAGC	2 cut(s)	62, 1553
BccI	CCATC	6 cut(s)	64, 132, 601, 809, 1142, 1217
BceAI	ACGGC	5 cut(s)	184, 275, 295, 312, 501
BciT130I	CCWGG	5 cut(s)	410, 682, 720, 878, 1336
BclI	TGATCA	3 cut(s)	796, 936, 1273
BcoDI	GTCTC	3 cut(s)	78, 122, 1191
BfmI	CTRYAG	1 cut(s)	630
BfoI	RGCGCY	4 cut(s)	56, 200, 551, 1533
BglI	GCCNNNNNGGC	1 cut(s)	296
BglII	AGATCT	1 cut(s)	451
BisI	GCNGC	4 cut(s)	15, 24, 51, 1542
BlpI	GCTNAGC	1 cut(s)	512
BlsI	GCNGC	4 cut(s)	16, 25, 52, 1543
Bme1390I	CCNGG	5 cut(s)	410, 682, 720, 878, 1336
BmeT110I	CYCGRG	4 cut(s)	77, 173, 590, 1263
BmgBI	CACGTC	1 cut(s)	318
BmgT120I	GGNCC	3 cut(s)	141, 1330, 1331
BmiI	GGNNCC	6 cut(s)	171, 198, 213, 1177, 1332, 1333
BmrFI	CCNGG	5 cut(s)	410, 682, 720, 878, 1336
BmrI	ACTGGG	1 cut(s)	1076
BmsI	GCATC	2 cut(s)	372, 498
BmuI	ACTGGG	1 cut(s)	1076
BpiI	GAAGAC	1 cut(s)	279
BpmI	CTGGAG	1 cut(s)	1473
Bpu10I	CCTNAGC	2 cut(s)	462, 810
Bpu1102I	GCTNAGC	1 cut(s)	512
Bpu14I	TTCGAA	1 cut(s)	1524
BsaAI	YACGTR	1 cut(s)	1343
BsaBI	GATNNNNATC	1 cut(s)	342
BsaHI	GRCGYC	2 cut(s)	197, 1041
BsaJI	CCNNGG	3 cut(s)	78, 669, 1334
BsaWI	WCCGGW	2 cut(s)	163, 214
BsaXI	ACNNNNNCTCC	4 cut(s)	79, 82, 109, 112
Bsc4I	CCNNNNNNNGG	1 cut(s)	166
Bse118I	RCCGGY	2 cut(s)	103, 299
Bse1I	ACTGG	3 cut(s)	689, 1071, 1490
Bse21I	CCTNAGG	1 cut(s)	543
Bse8I	GATNNNNATC	1 cut(s)	342
BseAI	TCCGGA	1 cut(s)	163
BseBI	CCWGG	5 cut(s)	410, 682, 720, 878, 1336
BseDI	CCNNGG	3 cut(s)	78, 669, 1334
BseGI	GGATG	4 cut(s)	513, 820, 832, 1453
BseJI	GATNNNNATC	1 cut(s)	342
BseLI	CCNNNNNNNGG	1 cut(s)	166
BseMII	CTCAG	5 cut(s)	108, 476, 526, 557, 801
BseNI	ACTGG	3 cut(s)	689, 1071, 1490
BseRI	GAGGAG	3 cut(s)	87, 182, 531
BseSI	GKGCMC	2 cut(s)	1180, 1334
BseX3I	CGGCCG	1 cut(s)	297
BseXI	GCAGC	2 cut(s)	62, 1553
Bsh1285I	CGRYCG	1 cut(s)	300
BshFI	GGCC	5 cut(s)	143, 299, 578, 685, 1332
BshNI	GGYRCC	2 cut(s)	196, 1175
BsiEI	CGRYCG	1 cut(s)	300
BsiHKCI	CYCGRG	4 cut(s)	77, 173, 590, 1263
BsiSI	CCGG	5 cut(s)	104, 164, 194, 215, 300
BslI	CCNNNNNNNGG	1 cut(s)	166
BsmAI	GTCTC	3 cut(s)	78, 122, 1191
BsmBI	CGTCTC	1 cut(s)	78
BsnI	GGCC	5 cut(s)	143, 299, 578, 685, 1332
BsoBI	CYCGRG	4 cut(s)	77, 173, 590, 1263
Bsp119I	TTCGAA	1 cut(s)	1524
Bsp120I	GGGCCC	1 cut(s)	1330
Bsp1286I	GDGCHC	3 cut(s)	174, 1180, 1334
Bsp13I	TCCGGA	1 cut(s)	163
Bsp1407I	TGTACA	1 cut(s)	1141
Bsp143I	GATC	7 cut(s)	337, 451, 523, 796, 936, 1273, 1345
Bsp1720I	GCTNAGC	1 cut(s)	512
BspACI	CCGC	8 cut(s)	15, 21, 24, 48, 101, 107, 1418, 1437
BspANI	GGCC	5 cut(s)	143, 299, 578, 685, 1332
BspCNI	CTCAG	5 cut(s)	109, 475, 525, 556, 802
BspEI	TCCGGA	1 cut(s)	163
BspLI	GGNNCC	6 cut(s)	171, 198, 213, 1177, 1332, 1333
BspMAI	CTGCAG	1 cut(s)	634
BspPI	GGATC	3 cut(s)	345, 518, 1340
BspQI	GCTCTTC	1 cut(s)	969
BspT104I	TTCGAA	1 cut(s)	1524
BspT107I	GGYRCC	2 cut(s)	196, 1175
BsrFI	RCCGGY	2 cut(s)	103, 299
BsrGI	TGTACA	1 cut(s)	1141
BsrI	ACTGG	3 cut(s)	689, 1071, 1490
BssAI	RCCGGY	2 cut(s)	103, 299
BssECI	CCNNGG	3 cut(s)	78, 669, 1334
BssMI	GATC	7 cut(s)	337, 451, 523, 796, 936, 1273, 1345
BssNAI	GTATAC	1 cut(s)	618
BssNI	GRCGYC	2 cut(s)	197, 1041
Bst1107I	GTATAC	1 cut(s)	618
Bst2UI	CCWGG	5 cut(s)	410, 682, 720, 878, 1336
Bst4CI	ACNGT	3 cut(s)	40, 1016, 1554
Bst6I	CTCTTC	2 cut(s)	144, 969
BstACI	GRCGYC	2 cut(s)	197, 1041
BstAPI	GCANNNNNTGC	1 cut(s)	638
BstAUI	TGTACA	1 cut(s)	1141
BstBAI	YACGTR	1 cut(s)	1343
BstBI	TTCGAA	1 cut(s)	1524
BstC8I	GCNNGC	3 cut(s)	105, 807, 1546
BstDEI	CTNAG	6 cut(s)	6, 117, 462, 512, 543, 810
BstEII	GGTNACC	1 cut(s)	950
BstF5I	GGATG	4 cut(s)	513, 820, 832, 1453
BstH2I	RGCGCY	4 cut(s)	56, 200, 551, 1533
BstHHI	GCGC	5 cut(s)	55, 199, 469, 550, 1532
BstKTI	GATC	7 cut(s)	340, 454, 526, 799, 939, 1276, 1348
BstMAI	GTCTC	3 cut(s)	78, 122, 1191
BstMBI	GATC	7 cut(s)	337, 451, 523, 796, 936, 1273, 1345
BstMCI	CGRYCG	1 cut(s)	300
BstNI	CCWGG	5 cut(s)	410, 682, 720, 878, 1336
BstNSI	RCATGY	1 cut(s)	805
BstPI	GGTNACC	1 cut(s)	950
BstSCI	CCNGG	5 cut(s)	408, 680, 718, 876, 1334
BstSFI	CTRYAG	1 cut(s)	630
BstSLI	GKGCMC	2 cut(s)	1180, 1334
BstV1I	GCAGC	2 cut(s)	62, 1553
BstV2I	GAAGAC	1 cut(s)	279
BstX2I	RGATCY	2 cut(s)	337, 451
BstXI	CCANNNNNNTGG	1 cut(s)	574
BstYI	RGATCY	2 cut(s)	337, 451
BstZ17I	GTATAC	1 cut(s)	618
BstZI	CGGCCG	1 cut(s)	297
Bsu36I	CCTNAGG	1 cut(s)	543
BsuRI	GGCC	5 cut(s)	143, 299, 578, 685, 1332
BtrI	CACGTC	1 cut(s)	318
BtsCI	GGATG	4 cut(s)	513, 820, 832, 1453
BtsI	GCAGTG	1 cut(s)	871
BtsIMutI	CAGTG	2 cut(s)	292, 871
Cac8I	GCNNGC	3 cut(s)	105, 807, 1546
CfoI	GCGC	5 cut(s)	55, 199, 469, 550, 1532
Cfr10I	RCCGGY	2 cut(s)	103, 299
Cfr13I	GGNCC	3 cut(s)	141, 1330, 1331
CseI	GACGC	1 cut(s)	197
Csp6I	GTAC	7 cut(s)	313, 359, 723, 1003, 1012, 1142, 1372
CspCI	CAANNNNNGTGG	2 cut(s)	928, 963
CviAII	CATG	2 cut(s)	802, 1459
CviQI	GTAC	7 cut(s)	313, 359, 723, 1003, 1012, 1142, 1372
DdeI	CTNAG	6 cut(s)	6, 117, 462, 512, 543, 810
DinI	GGCGCC	1 cut(s)	198
DpnI	GATC	7 cut(s)	339, 453, 525, 798, 938, 1275, 1347
DpnII	GATC	7 cut(s)	337, 451, 523, 796, 936, 1273, 1345
DrdI	GACNNNNNNGTC	2 cut(s)	1205, 1404
DseDI	GACNNNNNNGTC	2 cut(s)	1205, 1404
EaeI	YGGCCR	2 cut(s)	297, 576
EagI	CGGCCG	1 cut(s)	297
Eam1104I	CTCTTC	2 cut(s)	144, 969
EarI	CTCTTC	2 cut(s)	144, 969
EciI	GGCGGA	2 cut(s)	90, 122
EclXI	CGGCCG	1 cut(s)	297
Eco24I	GRGCYC	2 cut(s)	174, 1334
Eco52I	CGGCCG	1 cut(s)	297
Eco57I	CTGAAG	3 cut(s)	383, 584, 1209
Eco72I	CACGTG	1 cut(s)	1343
Eco81I	CCTNAGG	1 cut(s)	543
Eco88I	CYCGRG	4 cut(s)	77, 173, 590, 1263
Eco91I	GGTNACC	1 cut(s)	950
EcoO109I	RGGNCCY	2 cut(s)	1330, 1331
EcoO65I	GGTNACC	1 cut(s)	950
EcoRII	CCWGG	5 cut(s)	408, 680, 718, 876, 1334
EcoT38I	GRGCYC	2 cut(s)	174, 1334
EgeI	GGCGCC	1 cut(s)	198
EheI	GGCGCC	1 cut(s)	198
Esp3I	CGTCTC	1 cut(s)	78
FaeI	CATG	2 cut(s)	805, 1462
FatI	CATG	2 cut(s)	801, 1458
FauI	CCCGC	2 cut(s)	28, 55
FbaI	TGATCA	3 cut(s)	796, 936, 1273
FblI	GTMKAC	3 cut(s)	203, 617, 743
Fnu4HI	GCNGC	4 cut(s)	15, 24, 51, 1542
FokI	GGATG	4 cut(s)	520, 827, 839, 1460
FriOI	GRGCYC	2 cut(s)	174, 1334
Fsp4HI	GCNGC	4 cut(s)	15, 24, 51, 1542
GlaI	GCGC	5 cut(s)	54, 198, 468, 549, 1531
GluI	GCNGC	4 cut(s)	15, 24, 51, 1542
GsuI	CTGGAG	1 cut(s)	1473
HaeII	RGCGCY	4 cut(s)	56, 200, 551, 1533
HaeIII	GGCC	5 cut(s)	143, 299, 578, 685, 1332
HapII	CCGG	5 cut(s)	104, 164, 194, 215, 300
HgaI	GACGC	1 cut(s)	197
HhaI	GCGC	5 cut(s)	55, 199, 469, 550, 1532
Hin1I	GRCGYC	2 cut(s)	197, 1041
Hin1II	CATG	2 cut(s)	805, 1462
Hin6I	GCGC	5 cut(s)	53, 197, 467, 548, 1530
HinP1I	GCGC	5 cut(s)	53, 197, 467, 548, 1530
HincII	GTYRAC	1 cut(s)	744
HindII	GTYRAC	1 cut(s)	744
HindIII	AAGCTT	1 cut(s)	1518
HinfI	GANTC	6 cut(s)	8, 275, 343, 586, 775, 830
HpaII	CCGG	5 cut(s)	104, 164, 194, 215, 300
HphI	GGTGA	2 cut(s)	314, 1451
Hpy166II	GTNNAC	7 cut(s)	204, 223, 315, 618, 744, 1012, 1372
Hpy188I	TCNGA	7 cut(s)	127, 211, 342, 585, 603, 1104, 1536
Hpy188III	TCNNGA	9 cut(s)	60, 77, 164, 379, 590, 772, 916, 1019, 1352
Hpy8I	GTNNAC	7 cut(s)	204, 223, 315, 618, 744, 1012, 1372
Hpy99I	CGWCG	6 cut(s)	149, 191, 266, 322, 748, 791
HpyAV	CCTTC	4 cut(s)	358, 1319, 1383, 1520
HpyCH4III	ACNGT	3 cut(s)	40, 1016, 1554
HpyCH4IV	ACGT	4 cut(s)	317, 1041, 1342, 1399
HpyCH4V	TGCA	6 cut(s)	385, 555, 632, 1118, 1157, 1378
HpyF3I	CTNAG	6 cut(s)	6, 117, 462, 512, 543, 810
HpySE526I	ACGT	4 cut(s)	317, 1041, 1342, 1399
Hsp92I	GRCGYC	2 cut(s)	197, 1041
Hsp92II	CATG	2 cut(s)	805, 1462
HspAI	GCGC	5 cut(s)	53, 197, 467, 548, 1530
KasI	GGCGCC	1 cut(s)	196
Kpn2I	TCCGGA	1 cut(s)	163
KroI	GCCGGC	1 cut(s)	103
KroNI	GCCGGC	1 cut(s)	105
Ksp22I	TGATCA	3 cut(s)	796, 936, 1273
Kzo9I	GATC	7 cut(s)	337, 451, 523, 796, 936, 1273, 1345
LguI	GCTCTTC	1 cut(s)	969
LmnI	GCTCC	3 cut(s)	169, 196, 544
Lsp1109I	GCAGC	2 cut(s)	62, 1553
LweI	GCATC	2 cut(s)	372, 498
MaeII	ACGT	4 cut(s)	317, 1041, 1342, 1399
MaeIII	GTNAC	8 cut(s)	28, 292, 302, 913, 950, 1125, 1159, 1492
MalI	GATC	7 cut(s)	339, 453, 525, 798, 938, 1275, 1347
MboI	GATC	7 cut(s)	337, 451, 523, 796, 936, 1273, 1345
MboII	GAAGA	9 cut(s)	161, 190, 250, 284, 590, 616, 986, 1202, 1526
MflI	RGATCY	2 cut(s)	337, 451
MhlI	GDGCHC	3 cut(s)	174, 1180, 1334
MlsI	TGGCCA	1 cut(s)	578
MluNI	TGGCCA	1 cut(s)	578
Mly113I	GGCGCC	1 cut(s)	197
MlyI	GAGTC	2 cut(s)	17, 269
MmeI	TCCRAC	1 cut(s)	105
Mox20I	TGGCCA	1 cut(s)	578
MreI	CGCCGGCG	1 cut(s)	103
MroI	TCCGGA	1 cut(s)	163
MroNI	GCCGGC	1 cut(s)	103
MroXI	GAANNNNTTC	1 cut(s)	254
MscI	TGGCCA	1 cut(s)	578
MseI	TTAA	8 cut(s)	729, 737, 822, 903, 1229, 1287, 1425, 1482
Msp20I	TGGCCA	1 cut(s)	578
MspA1I	CMGCKG	2 cut(s)	26, 1544
MspI	CCGG	5 cut(s)	104, 164, 194, 215, 300
MspR9I	CCNGG	5 cut(s)	410, 682, 720, 878, 1336
MvaI	CCWGG	5 cut(s)	410, 682, 720, 878, 1336
NaeI	GCCGGC	1 cut(s)	105
NarI	GGCGCC	1 cut(s)	197
NdeII	GATC	7 cut(s)	337, 451, 523, 796, 936, 1273, 1345
NgoMIV	GCCGGC	1 cut(s)	103
NlaIII	CATG	2 cut(s)	805, 1462
NlaIV	GGNNCC	6 cut(s)	171, 198, 213, 1177, 1332, 1333
NmeAIII	GCCGAG	2 cut(s)	423, 650
NmuCI	GTSAC	3 cut(s)	28, 302, 913
NspI	RCATGY	1 cut(s)	805
NspV	TTCGAA	1 cut(s)	1524
PaeR7I	CTCGAG	2 cut(s)	590, 1263
PciSI	GCTCTTC	1 cut(s)	969
PcsI	WCGNNNNNNNCGW	3 cut(s)	153, 267, 314
PdiI	GCCGGC	1 cut(s)	105
PdmI	GAANNNNTTC	1 cut(s)	254
PfeI	GAWTC	4 cut(s)	343, 586, 775, 830
PflFI	GACNNNGTC	1 cut(s)	1459
PkrI	GCNGC	4 cut(s)	16, 25, 52, 1543
PleI	GAGTC	2 cut(s)	16, 269
PluTI	GGCGCC	1 cut(s)	200
PmaCI	CACGTG	1 cut(s)	1343
PmlI	CACGTG	1 cut(s)	1343
PpsI	GAGTC	2 cut(s)	16, 269
Ppu21I	YACGTR	1 cut(s)	1343
PshBI	ATTAAT	2 cut(s)	737, 1287
Psp6I	CCWGG	5 cut(s)	408, 680, 718, 876, 1334
PspCI	CACGTG	1 cut(s)	1343
PspEI	GGTNACC	1 cut(s)	950
PspGI	CCWGG	5 cut(s)	408, 680, 718, 876, 1334
PspN4I	GGNNCC	6 cut(s)	171, 198, 213, 1177, 1332, 1333
PspOMI	GGGCCC	1 cut(s)	1330
PspPI	GGNCC	3 cut(s)	141, 1330, 1331
PspXI	VCTCGAGB	1 cut(s)	1263
PsrI	GAACNNNNNNTAC	2 cut(s)	706, 738
PstI	CTGCAG	1 cut(s)	634
PsuI	RGATCY	2 cut(s)	337, 451
PsyI	GACNNNGTC	1 cut(s)	1459
PvuII	CAGCTG	1 cut(s)	1544
RsaI	GTAC	7 cut(s)	314, 360, 724, 1004, 1013, 1143, 1373
RsaNI	GTAC	7 cut(s)	313, 359, 723, 1003, 1012, 1142, 1372
SalI	GTCGAC	1 cut(s)	742
SapI	GCTCTTC	1 cut(s)	969
SaqAI	TTAA	8 cut(s)	729, 737, 822, 903, 1229, 1287, 1425, 1482
SatI	GCNGC	4 cut(s)	15, 24, 51, 1542
Sau3AI	GATC	7 cut(s)	337, 451, 523, 796, 936, 1273, 1345
Sau96I	GGNCC	3 cut(s)	141, 1330, 1331
SchI	GAGTC	2 cut(s)	17, 269
ScrFI	CCNGG	5 cut(s)	410, 682, 720, 878, 1336
SduI	GDGCHC	3 cut(s)	174, 1180, 1334
SfaNI	GCATC	2 cut(s)	372, 498
SfcI	CTRYAG	1 cut(s)	630
SfoI	GGCGCC	1 cut(s)	198
Sfr274I	CTCGAG	2 cut(s)	590, 1263
SfuI	TTCGAA	1 cut(s)	1524
SgrAI	CRCCGGYG	1 cut(s)	103
SlaI	CTCGAG	2 cut(s)	590, 1263
SmlI	CTYRAG	2 cut(s)	590, 1263
SmoI	CTYRAG	2 cut(s)	590, 1263
SsiI	CCGC	8 cut(s)	15, 21, 24, 48, 101, 107, 1418, 1437
SspDI	GGCGCC	1 cut(s)	196
SspI	AATATT	1 cut(s)	1059
StyD4I	CCNGG	5 cut(s)	408, 680, 718, 876, 1334
TaaI	ACNGT	3 cut(s)	40, 1016, 1554
TaiI	ACGT	4 cut(s)	320, 1044, 1345, 1402
TaqI	TCGA	9 cut(s)	261, 333, 378, 522, 591, 743, 786, 1264, 1524
TatI	WGTACW	5 cut(s)	358, 1002, 1011, 1141, 1371
TauI	GCSGC	2 cut(s)	17, 26
TfiI	GAWTC	4 cut(s)	343, 586, 775, 830
Tru1I	TTAA	8 cut(s)	729, 737, 822, 903, 1229, 1287, 1425, 1482
Tru9I	TTAA	8 cut(s)	729, 737, 822, 903, 1229, 1287, 1425, 1482
TscAI	CASTG	2 cut(s)	292, 878
TseFI	GTSAC	3 cut(s)	28, 302, 913
TseI	GCWGC	2 cut(s)	50, 1541
Tsp45I	GTSAC	3 cut(s)	28, 302, 913
TspDTI	ATGAA	4 cut(s)	860, 921, 1038, 1464
TspRI	CASTG	2 cut(s)	292, 878
Tth111I	GACNNNGTC	1 cut(s)	1459
VspI	ATTAAT	2 cut(s)	737, 1287
XceI	RCATGY	1 cut(s)	805
XcmI	CCANNNNNNNNNTGG	1 cut(s)	571
XhoI	CTCGAG	2 cut(s)	590, 1263
XmiI	GTMKAC	3 cut(s)	203, 617, 743
XmnI	GAANNNNTTC	1 cut(s)	254
ZraI	GACGTC	1 cut(s)	1042

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.