MD12G1113600.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0002165
Physical Location & Seq
Reverse (-)
18084181 .. 18085173
993 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1113600.v1.1.491

Sequence Viewer

Length: 300 bp
ATGGAGAAGTACTTCGGAAACGCATACAGAGGCGACCCGGGGGTCCCACACGCCGACCCGGAACGATTTGTGAACATATGGATCGGGTCCGCCGCCTTCTCAGCCCTCACCTGGGTCAATCCCTACATGTGGCAGCTCTCCAATCAGTTCAATTGGCATGACAAAGCAATGCTGTTTGAGCAGTACCACTGGAAGAAGGCCATGAAGAAAGGGGAGCCCTACAAATTTAAGTGGAACGAGTACATGGACAGGGACCACAGGGAGTCCTACTATTTCAACTGGCCTGTTTACTTCCCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

100

Amino Acids

12.23

Weight (kDa)

7.94

Isoelectric Point (pI)

25.64

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 41
AciI CCGC 2 cut(s) 90, 93
AclWI GGATC 1 cut(s) 89
AcsI RAATTY 1 cut(s) 224
AfaI GTAC 3 cut(s) 11, 185, 242
AfiI CCNNNNNNNGG 3 cut(s) 111, 112, 129
AflIII ACRYGT 1 cut(s) 126
AgsI TTSAA 2 cut(s) 151, 277
AjnI CCWGG 1 cut(s) 110
AluBI AGCT 1 cut(s) 136
AluI AGCT 1 cut(s) 136
AlwI GGATC 1 cut(s) 89
Ama87I CYCGRG 1 cut(s) 37
AoxI GGCC 2 cut(s) 198, 281
ApeKI GCWGC 1 cut(s) 133
ApoI RAATTY 1 cut(s) 224
Asp700I GAANNNNTTC 1 cut(s) 11
AspS9I GGNCC 3 cut(s) 43, 87, 253
AsuC2I CCSGG 3 cut(s) 38, 39, 59
AsuHPI GGTGA 1 cut(s) 100
AvaI CYCGRG 1 cut(s) 37
AvaII GGWCC 3 cut(s) 43, 87, 253
BanII GRGCYC 1 cut(s) 219
BbvI GCAGC 1 cut(s) 145
BciT130I CCWGG 1 cut(s) 112
BcnI CCSGG 3 cut(s) 38, 39, 59
BisI GCNGC 2 cut(s) 93, 134
BlsI GCNGC 2 cut(s) 94, 135
BmcAI AGTACT 1 cut(s) 11
Bme1390I CCNGG 4 cut(s) 38, 39, 59, 112
Bme18I GGWCC 3 cut(s) 43, 87, 253
BmeT110I CYCGRG 1 cut(s) 37
BmgT120I GGNCC 3 cut(s) 43, 87, 253
BmiI GGNNCC 5 cut(s) 44, 45, 88, 216, 254
BmrFI CCNGG 4 cut(s) 38, 39, 59, 112
BpuMI CCSGG 3 cut(s) 38, 39, 59
BsaJI CCNNGG 3 cut(s) 37, 38, 111
Bsc4I CCNNNNNNNGG 3 cut(s) 111, 112, 129
Bse1I ACTGG 2 cut(s) 194, 284
Bse3DI GCAATG 1 cut(s) 174
BseBI CCWGG 1 cut(s) 112
BseDI CCNNGG 3 cut(s) 37, 38, 111
BseLI CCNNNNNNNGG 3 cut(s) 111, 112, 129
BseMI GCAATG 1 cut(s) 174
BseMII CTCAG 1 cut(s) 114
BseNI ACTGG 2 cut(s) 194, 284
BseXI GCAGC 1 cut(s) 145
BshFI GGCC 2 cut(s) 200, 283
BsiHKCI CYCGRG 1 cut(s) 37
BsiSI CCGG 2 cut(s) 38, 59
BslFI GGGAC 2 cut(s) 29, 266
BslI CCNNNNNNNGG 3 cut(s) 111, 112, 129
BsmFI GGGAC 2 cut(s) 29, 266
BsnI GGCC 2 cut(s) 200, 283
BsoBI CYCGRG 1 cut(s) 37
Bsp1286I GDGCHC 1 cut(s) 219
Bsp143I GATC 1 cut(s) 81
BspACI CCGC 2 cut(s) 90, 93
BspANI GGCC 2 cut(s) 200, 283
BspCNI CTCAG 1 cut(s) 113
BspLI GGNNCC 5 cut(s) 44, 45, 88, 216, 254
BspPI GGATC 1 cut(s) 89
BsrDI GCAATG 1 cut(s) 174
BsrI ACTGG 2 cut(s) 194, 284
BssECI CCNNGG 3 cut(s) 37, 38, 111
BssMI GATC 1 cut(s) 81
Bst2UI CCWGG 1 cut(s) 112
BstDEI CTNAG 2 cut(s) 100, 297
BstKTI GATC 1 cut(s) 84
BstMBI GATC 1 cut(s) 81
BstMWI GCNNNNNNNGC 2 cut(s) 101, 178
BstNI CCWGG 1 cut(s) 112
BstNSI RCATGY 1 cut(s) 130
BstSCI CCNGG 4 cut(s) 36, 37, 57, 110
BstV1I GCAGC 1 cut(s) 145
BsuRI GGCC 2 cut(s) 200, 283
BtsIMutI CAGTG 1 cut(s) 187
Cfr13I GGNCC 3 cut(s) 43, 87, 253
Cfr9I CCCGGG 1 cut(s) 37
Csp6I GTAC 3 cut(s) 10, 184, 241
CviAII CATG 4 cut(s) 127, 158, 202, 244
CviJI RGCY 5 cut(s) 104, 136, 200, 217, 283
CviKI_1 RGCY 5 cut(s) 104, 136, 200, 217, 283
CviQI GTAC 3 cut(s) 10, 184, 241
DdeI CTNAG 2 cut(s) 100, 297
DpnI GATC 1 cut(s) 83
DpnII GATC 1 cut(s) 81
DrdI GACNNNNNNGTC 1 cut(s) 41
DseDI GACNNNNNNGTC 1 cut(s) 41
EciI GGCGGA 1 cut(s) 79
Eco24I GRGCYC 1 cut(s) 219
Eco47I GGWCC 3 cut(s) 43, 87, 253
Eco88I CYCGRG 1 cut(s) 37
EcoO109I RGGNCCY 1 cut(s) 43
EcoRII CCWGG 1 cut(s) 110
EcoT38I GRGCYC 1 cut(s) 219
FaeI CATG 4 cut(s) 130, 161, 205, 247
FaiI YATR 7 cut(s) 25, 77, 79, 128, 159, 203, 245
FaqI GGGAC 2 cut(s) 29, 266
FatI CATG 4 cut(s) 126, 157, 201, 243
FauNDI CATATG 1 cut(s) 77
Fnu4HI GCNGC 2 cut(s) 93, 134
FriOI GRGCYC 1 cut(s) 219
Fsp4HI GCNGC 2 cut(s) 93, 134
GluI GCNGC 2 cut(s) 93, 134
HaeIII GGCC 2 cut(s) 200, 283
HapII CCGG 2 cut(s) 38, 59
Hin1II CATG 4 cut(s) 130, 161, 205, 247
HinfI GANTC 1 cut(s) 263
HpaII CCGG 2 cut(s) 38, 59
HphI GGTGA 1 cut(s) 100
Hpy166II GTNNAC 2 cut(s) 73, 289
Hpy188I TCNGA 1 cut(s) 17
Hpy8I GTNNAC 2 cut(s) 73, 289
HpyAV CCTTC 2 cut(s) 106, 190
HpyF10VI GCNNNNNNNGC 2 cut(s) 101, 178
HpyF3I CTNAG 2 cut(s) 100, 297
Hsp92II CATG 4 cut(s) 130, 161, 205, 247
KflI GGGWCCC 1 cut(s) 43
Kzo9I GATC 1 cut(s) 81
LmnI GCTCC 1 cut(s) 214
LpnPI CCDG 8 cut(s) 51, 72, 97, 124, 175, 235, 244, 265
Lsp1109I GCAGC 1 cut(s) 145
MalI GATC 1 cut(s) 83
MboI GATC 1 cut(s) 81
MboII GAAGA 2 cut(s) 205, 217
MfeI CAATTG 1 cut(s) 151
MhlI GDGCHC 1 cut(s) 219
MluCI AATT 2 cut(s) 151, 224
MlyI GAGTC 1 cut(s) 272
MnlI CCTC 2 cut(s) 23, 116
MroXI GAANNNNTTC 1 cut(s) 11
MseI TTAA 1 cut(s) 228
MspI CCGG 2 cut(s) 38, 59
MspR9I CCNGG 4 cut(s) 38, 39, 59, 112
MunI CAATTG 1 cut(s) 151
MvaI CCWGG 1 cut(s) 112
MwoI GCNNNNNNNGC 2 cut(s) 101, 178
NciI CCSGG 3 cut(s) 38, 39, 59
NdeI CATATG 1 cut(s) 77
NdeII GATC 1 cut(s) 81
NlaIII CATG 4 cut(s) 130, 161, 205, 247
NlaIV GGNNCC 5 cut(s) 44, 45, 88, 216, 254
NspI RCATGY 1 cut(s) 130
PciI ACATGT 1 cut(s) 126
PdmI GAANNNNTTC 1 cut(s) 11
PkrI GCNGC 2 cut(s) 94, 135
PleI GAGTC 1 cut(s) 271
PpsI GAGTC 1 cut(s) 271
PpuMI RGGWCCY 1 cut(s) 43
PscI ACATGT 1 cut(s) 126
Psp5II RGGWCCY 1 cut(s) 43
Psp6I CCWGG 1 cut(s) 110
PspGI CCWGG 1 cut(s) 110
PspN4I GGNNCC 5 cut(s) 44, 45, 88, 216, 254
PspPI GGNCC 3 cut(s) 43, 87, 253
PspPPI RGGWCCY 1 cut(s) 43
RsaI GTAC 3 cut(s) 11, 185, 242
RsaNI GTAC 3 cut(s) 10, 184, 241
SaqAI TTAA 1 cut(s) 228
SatI GCNGC 2 cut(s) 93, 134
Sau3AI GATC 1 cut(s) 81
Sau96I GGNCC 3 cut(s) 43, 87, 253
ScaI AGTACT 1 cut(s) 11
SchI GAGTC 1 cut(s) 272
ScrFI CCNGG 4 cut(s) 38, 39, 59, 112
SduI GDGCHC 1 cut(s) 219
SetI ASST 2 cut(s) 113, 138
SinI GGWCC 3 cut(s) 43, 87, 253
SmaI CCCGGG 1 cut(s) 39
Sse9I AATT 2 cut(s) 151, 224
SsiI CCGC 2 cut(s) 90, 93
StyD4I CCNGG 4 cut(s) 36, 37, 57, 110
TasI AATT 2 cut(s) 151, 224
TatI WGTACW 2 cut(s) 9, 240
TauI GCSGC 1 cut(s) 95
Tru1I TTAA 1 cut(s) 228
Tru9I TTAA 1 cut(s) 228
TscAI CASTG 1 cut(s) 194
TseI GCWGC 1 cut(s) 133
TspDTI ATGAA 1 cut(s) 218
TspMI CCCGGG 1 cut(s) 37
TspRI CASTG 1 cut(s) 194
VpaK11BI GGWCC 3 cut(s) 43, 87, 253
XapI RAATTY 1 cut(s) 224
XceI RCATGY 1 cut(s) 130
XmaI CCCGGG 1 cut(s) 37
XmnI GAANNNNTTC 1 cut(s) 11
ZrmI AGTACT 1 cut(s) 11
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.