Rw0G008550

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00325
OG0002165
Physical Location & Seq
Forward (+)
5061 .. 5571
511 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G008550.1

Sequence Viewer

Length: 219 bp
ATGGATCGGATCCGCTGCCTTCCCTTCTCTGTGCTGACCTTGTTCAATCCCTACATGTGGCAGCTTTCCAATCGGTTCAATTGGCATGACAAGGCAATGCTGTTTGAGCAGCATCACTGGAAAAAGGCACTGAAGAAAGGGCAGCCCTATCAATTTAAGTACATGGACAAGGACCTCAGAGATGCCTACTATATCAACTGGCCGGTTTATTTCCCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

72

Amino Acids

9.11

Weight (kDa)

9.52

Isoelectric Point (pI)

34.84

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 13
AclWI GGATC 3 cut(s) 4, 12, 17
AcoI YGGCCR 1 cut(s) 200
AcuI CTGAAG 1 cut(s) 152
AfaI GTAC 1 cut(s) 161
AfiI CCNNNNNNNGG 1 cut(s) 57
AflIII ACRYGT 1 cut(s) 54
AgsI TTSAA 2 cut(s) 46, 79
AluBI AGCT 1 cut(s) 64
AluI AGCT 1 cut(s) 64
AlwI GGATC 3 cut(s) 4, 12, 17
AoxI GGCC 1 cut(s) 200
ApeKI GCWGC 4 cut(s) 15, 61, 109, 142
AspS9I GGNCC 1 cut(s) 172
AvaII GGWCC 1 cut(s) 172
BamHI GGATCC 1 cut(s) 9
BbvI GCAGC 4 cut(s) 2, 73, 121, 154
BisI GCNGC 4 cut(s) 16, 62, 110, 143
BlsI GCNGC 4 cut(s) 17, 63, 111, 144
Bme18I GGWCC 1 cut(s) 172
BmgT120I GGNCC 1 cut(s) 172
BmiI GGNNCC 1 cut(s) 11
BmsI GCATC 2 cut(s) 121, 172
Bsc4I CCNNNNNNNGG 1 cut(s) 57
Bse118I RCCGGY 1 cut(s) 202
Bse1I ACTGG 2 cut(s) 122, 203
Bse3DI GCAATG 1 cut(s) 102
BseLI CCNNNNNNNGG 1 cut(s) 57
BseMI GCAATG 1 cut(s) 102
BseMII CTCAG 1 cut(s) 190
BseNI ACTGG 2 cut(s) 122, 203
BseXI GCAGC 4 cut(s) 2, 73, 121, 154
BshFI GGCC 1 cut(s) 202
BsiSI CCGG 1 cut(s) 203
BslI CCNNNNNNNGG 1 cut(s) 57
BsnI GGCC 1 cut(s) 202
Bsp143I GATC 2 cut(s) 4, 9
BspACI CCGC 1 cut(s) 13
BspANI GGCC 1 cut(s) 202
BspCNI CTCAG 1 cut(s) 189
BspLI GGNNCC 1 cut(s) 11
BspPI GGATC 3 cut(s) 4, 12, 17
BsrDI GCAATG 1 cut(s) 102
BsrFI RCCGGY 1 cut(s) 202
BsrI ACTGG 2 cut(s) 122, 203
BssAI RCCGGY 1 cut(s) 202
BssMI GATC 2 cut(s) 4, 9
BstDEI CTNAG 2 cut(s) 176, 216
BstKTI GATC 2 cut(s) 7, 12
BstMBI GATC 2 cut(s) 4, 9
BstMWI GCNNNNNNNGC 1 cut(s) 106
BstNSI RCATGY 1 cut(s) 58
BstV1I GCAGC 4 cut(s) 2, 73, 121, 154
BstX2I RGATCY 1 cut(s) 9
BstYI RGATCY 1 cut(s) 9
BsuRI GGCC 1 cut(s) 202
BtsIMutI CAGTG 2 cut(s) 115, 128
Cfr10I RCCGGY 1 cut(s) 202
Cfr13I GGNCC 1 cut(s) 172
Csp6I GTAC 1 cut(s) 160
CviAII CATG 3 cut(s) 55, 86, 163
CviJI RGCY 3 cut(s) 64, 145, 202
CviKI_1 RGCY 3 cut(s) 64, 145, 202
CviQI GTAC 1 cut(s) 160
DdeI CTNAG 2 cut(s) 176, 216
DpnI GATC 2 cut(s) 6, 11
DpnII GATC 2 cut(s) 4, 9
EaeI YGGCCR 1 cut(s) 200
Eco47I GGWCC 1 cut(s) 172
Eco57I CTGAAG 1 cut(s) 152
EcoO109I RGGNCCY 1 cut(s) 172
FaeI CATG 3 cut(s) 58, 89, 166
FaiI YATR 4 cut(s) 56, 87, 164, 192
FatI CATG 3 cut(s) 54, 85, 162
Fnu4HI GCNGC 4 cut(s) 16, 62, 110, 143
Fsp4HI GCNGC 4 cut(s) 16, 62, 110, 143
GluI GCNGC 4 cut(s) 16, 62, 110, 143
HaeIII GGCC 1 cut(s) 202
HapII CCGG 1 cut(s) 203
Hin1II CATG 3 cut(s) 58, 89, 166
HpaII CCGG 1 cut(s) 203
Hpy188I TCNGA 2 cut(s) 9, 179
HpyAV CCTTC 2 cut(s) 29, 34
HpyF10VI GCNNNNNNNGC 1 cut(s) 106
HpyF3I CTNAG 2 cut(s) 176, 216
Hsp92II CATG 3 cut(s) 58, 89, 166
Kzo9I GATC 2 cut(s) 4, 9
LpnPI CCDG 2 cut(s) 103, 184
Lsp1109I GCAGC 4 cut(s) 2, 73, 121, 154
LweI GCATC 2 cut(s) 121, 172
MalI GATC 2 cut(s) 6, 11
MboI GATC 2 cut(s) 4, 9
MboII GAAGA 1 cut(s) 145
MfeI CAATTG 1 cut(s) 79
MflI RGATCY 1 cut(s) 9
MluCI AATT 2 cut(s) 79, 152
MnlI CCTC 1 cut(s) 185
MseI TTAA 1 cut(s) 156
MspA1I CMGCKG 1 cut(s) 15
MspI CCGG 1 cut(s) 203
MunI CAATTG 1 cut(s) 79
MwoI GCNNNNNNNGC 1 cut(s) 106
NdeII GATC 2 cut(s) 4, 9
NlaIII CATG 3 cut(s) 58, 89, 166
NlaIV GGNNCC 1 cut(s) 11
NspI RCATGY 1 cut(s) 58
PciI ACATGT 1 cut(s) 54
PkrI GCNGC 4 cut(s) 17, 63, 111, 144
PpuMI RGGWCCY 1 cut(s) 172
PscI ACATGT 1 cut(s) 54
Psp5II RGGWCCY 1 cut(s) 172
PspN4I GGNNCC 1 cut(s) 11
PspPI GGNCC 1 cut(s) 172
PspPPI RGGWCCY 1 cut(s) 172
PsuI RGATCY 1 cut(s) 9
RsaI GTAC 1 cut(s) 161
RsaNI GTAC 1 cut(s) 160
SaqAI TTAA 1 cut(s) 156
SatI GCNGC 4 cut(s) 16, 62, 110, 143
Sau3AI GATC 2 cut(s) 4, 9
Sau96I GGNCC 1 cut(s) 172
SetI ASST 3 cut(s) 41, 66, 177
SfaNI GCATC 2 cut(s) 121, 172
SgeI CNNG 9 cut(s) 52, 67, 98, 103, 130, 175, 181, 211, 215
SinI GGWCC 1 cut(s) 172
Sse9I AATT 2 cut(s) 79, 152
SsiI CCGC 1 cut(s) 13
TasI AATT 2 cut(s) 79, 152
TatI WGTACW 1 cut(s) 159
Tru1I TTAA 1 cut(s) 156
Tru9I TTAA 1 cut(s) 156
TscAI CASTG 2 cut(s) 122, 135
TseI GCWGC 4 cut(s) 15, 61, 109, 142
TspRI CASTG 2 cut(s) 122, 135
VpaK11BI GGWCC 1 cut(s) 172
XceI RCATGY 1 cut(s) 58
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.