Rh4DG114400

No apical meristem-associated C-terminal domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0002165
Physical Location & Seq
Reverse (-)
18078452 .. 18080614
2163 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG114400.1

Sequence Viewer

Length: 1056 bp
ATGGATTCTGCTCAAGTCCCCCGTGTTGCAATAGATTTAGGAGAGGATATTTTAAATTATGCCAATATCCTCAATGGAAGCTCAGGGCATAATGATAATATGTTTTCAACGTTTGGTTATCCTGCATCTGGCGATGCTATTAGCTATAGTCAGAACCCTATTCAAAGTCAAGTTGCTGCTCAAAGTGAACCCAAAACCACAAAGAAAGCTAAAAGAGCCAAGAATTTCTCCATTCAAGAAGACAACCTTCTTGTGTCTGCCTGGCTCAATACTACCCTTGATCCAGCAATAGGAAATGATCAAAAAGGTGCTGCTTATTGGAAAAGAATTTGGGACTATTTCTATGCTGAGAAGAACTTTGAACTAGAGTGTGATCGTAATCAGGGTTCTCTCATGCATCGCTGGTCTGGAATTCAACTGGATGTGAACAAATTTTGTGGCTATATTGCTGAAATTGAAAGGACAAGAGCAAGTGGTACAACTGAACAAGATAGGATTGTGGAAGCCAAACAAAAGTTTAGGAGGGAGCGAGGATACAACTTTACATATGAGCATTGTTGGCATGTGTTGAAGTTCCACCCGAAATGGAACTTGGAACTCTCTAGGAAAAAACCAAAGAAAACTCATGCAACTGTTCCTGCTACTTTGTCTCCTATCACTCCATCTGCTAGCCCAGATACAATAGATTTAGCTGATGGCAATGTTGAAGGTAATGAGTCTCCATGCTTGGAGAGGCCTATAGGCAAAAAGGCTGCCAAGACCTTGGCAAGGAAGGCAAAAGCTAAAGAGAAAGTGGAAGCAGCCCAATCTGAATTAGGAGACTATTATGCTCTAAAAGTTGAACATGCTCATAAGGAGGATGAGCAATTTCAACTGATGTATGATGCAGAACAAGAGAACATTAAGCTGAGGAAACAAGAACTTGAAATTCAAGTTCGGAAAGAGGATAATGCAATAATGGCAATTGATACTTCTACAATGGAACCAATGCGGGCAGAATATTTTAGAGGACTTCAACAGGAAATCATAGCAAAAAGAGCTACTAATGGAAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NAM-associated

Protein Analysis

351

Amino Acids

39.88

Weight (kDa)

6.73

Isoelectric Point (pI)

50.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAM-associated PF14303 181 - 342 3e-23 No apical meristem-associated C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 991
AclI AACGTT 1 cut(s) 110
AclWI GGATC 1 cut(s) 275
AcsI RAATTY 5 cut(s) 223, 327, 411, 431, 927
AfaI GTAC 1 cut(s) 478
AfiI CCNNNNNNNGG 3 cut(s) 128, 290, 768
AjnI CCWGG 1 cut(s) 260
AjuI GAANNNNNNNTTGG 2 cut(s) 575, 607
AluBI AGCT 7 cut(s) 81, 144, 209, 692, 782, 907, 1040
AluI AGCT 7 cut(s) 81, 144, 209, 692, 782, 907, 1040
Alw26I GTCTC 3 cut(s) 654, 723, 813
AlwI GGATC 1 cut(s) 275
AoxI GGCC 1 cut(s) 734
ApeKI GCWGC 4 cut(s) 176, 311, 752, 800
ApoI RAATTY 5 cut(s) 223, 327, 411, 431, 927
AsuNHI GCTAGC 1 cut(s) 668
BbsI GAAGAC 1 cut(s) 246
BbvCI CCTCAGC 1 cut(s) 908
BbvI GCAGC 4 cut(s) 163, 298, 739, 812
BccI CCATC 2 cut(s) 670, 689
BciT130I CCWGG 1 cut(s) 262
BciVI GTATCC 1 cut(s) 527
BclI TGATCA 1 cut(s) 298
BcoDI GTCTC 3 cut(s) 654, 723, 813
BfaI CTAG 4 cut(s) 365, 603, 669, 1054
BfmI CTRYAG 2 cut(s) 145, 738
BfuI GTATCC 1 cut(s) 527
BisI GCNGC 4 cut(s) 177, 312, 753, 801
BlsI GCNGC 4 cut(s) 178, 313, 754, 802
Bme1390I CCNGG 1 cut(s) 262
BmiI GGNNCC 1 cut(s) 984
BmrFI CCNGG 1 cut(s) 262
BmsI GCATC 4 cut(s) 124, 134, 406, 874
BmtI GCTAGC 1 cut(s) 672
BpiI GAAGAC 1 cut(s) 246
Bpu10I CCTNAGC 2 cut(s) 82, 908
BsaBI GATNNNNATC 1 cut(s) 378
BsaJI CCNNGG 1 cut(s) 762
BsaXI ACNNNNNCTCC 2 cut(s) 634, 664
Bsc4I CCNNNNNNNGG 3 cut(s) 128, 290, 768
Bse1I ACTGG 1 cut(s) 423
Bse3DI GCAATG 1 cut(s) 706
Bse8I GATNNNNATC 1 cut(s) 378
BseBI CCWGG 1 cut(s) 262
BseDI CCNNGG 1 cut(s) 762
BseGI GGATG 2 cut(s) 427, 865
BseJI GATNNNNATC 1 cut(s) 378
BseLI CCNNNNNNNGG 3 cut(s) 128, 290, 768
BseMI GCAATG 1 cut(s) 706
BseMII CTCAG 3 cut(s) 96, 339, 899
BseNI ACTGG 1 cut(s) 423
BseXI GCAGC 4 cut(s) 163, 298, 739, 812
BshFI GGCC 1 cut(s) 736
BslFI GGGAC 2 cut(s) 2, 347
BslI CCNNNNNNNGG 3 cut(s) 128, 290, 768
BsmAI GTCTC 3 cut(s) 654, 723, 813
BsmFI GGGAC 2 cut(s) 2, 347
BsnI GGCC 1 cut(s) 736
Bsp143I GATC 3 cut(s) 280, 298, 373
BspACI CCGC 1 cut(s) 991
BspANI GGCC 1 cut(s) 736
BspCNI CTCAG 3 cut(s) 95, 340, 900
BspLI GGNNCC 1 cut(s) 984
BspOI GCTAGC 1 cut(s) 672
BspPI GGATC 1 cut(s) 275
BsrDI GCAATG 1 cut(s) 706
BsrI ACTGG 1 cut(s) 423
BssECI CCNNGG 1 cut(s) 762
BssMI GATC 3 cut(s) 280, 298, 373
BssT1I CCWWGG 1 cut(s) 762
Bst2UI CCWGG 1 cut(s) 262
Bst4CI ACNGT 1 cut(s) 634
BstC8I GCNNGC 2 cut(s) 670, 993
BstDEI CTNAG 3 cut(s) 82, 348, 908
BstENI CCTNNNNNAGG 1 cut(s) 766
BstF5I GGATG 2 cut(s) 427, 865
BstKTI GATC 3 cut(s) 283, 301, 376
BstMAI GTCTC 3 cut(s) 654, 723, 813
BstMBI GATC 3 cut(s) 280, 298, 373
BstMWI GCNNNNNNNGC 5 cut(s) 215, 559, 773, 959, 1037
BstNI CCWGG 1 cut(s) 262
BstNSI RCATGY 2 cut(s) 566, 848
BstSCI CCNGG 1 cut(s) 260
BstSFI CTRYAG 2 cut(s) 145, 738
BstV1I GCAGC 4 cut(s) 163, 298, 739, 812
BstV2I GAAGAC 1 cut(s) 246
BstXI CCANNNNNNTGG 1 cut(s) 763
BsuI GTATCC 1 cut(s) 527
BsuRI GGCC 1 cut(s) 736
BtgZI GCGATG 2 cut(s) 147, 383
BtsCI GGATG 2 cut(s) 427, 865
Cac8I GCNNGC 2 cut(s) 670, 993
Csp6I GTAC 1 cut(s) 477
CspCI CAANNNNNGTGG 2 cut(s) 418, 453
CviAII CATG 5 cut(s) 394, 563, 626, 723, 845
CviQI GTAC 1 cut(s) 477
DdeI CTNAG 3 cut(s) 82, 348, 908
DpnI GATC 3 cut(s) 282, 300, 375
DpnII GATC 3 cut(s) 280, 298, 373
DraI TTTAAA 1 cut(s) 54
Eco130I CCWWGG 1 cut(s) 762
Eco147I AGGCCT 1 cut(s) 736
EcoNI CCTNNNNNAGG 1 cut(s) 766
EcoRI GAATTC 1 cut(s) 411
EcoRII CCWGG 1 cut(s) 260
EcoT14I CCWWGG 1 cut(s) 762
EcoT22I ATGCAT 1 cut(s) 399
ErhI CCWWGG 1 cut(s) 762
FaeI CATG 5 cut(s) 397, 566, 629, 726, 848
FalI AAGNNNNNCTT 2 cut(s) 231, 263
FaqI GGGAC 2 cut(s) 2, 347
FatI CATG 5 cut(s) 393, 562, 625, 722, 844
FauI CCCGC 1 cut(s) 984
FauNDI CATATG 1 cut(s) 547
FbaI TGATCA 1 cut(s) 298
Fnu4HI GCNGC 4 cut(s) 177, 312, 753, 801
FokI GGATG 2 cut(s) 434, 872
Fsp4HI GCNGC 4 cut(s) 177, 312, 753, 801
FspBI CTAG 4 cut(s) 365, 603, 669, 1054
GluI GCNGC 4 cut(s) 177, 312, 753, 801
HaeIII GGCC 1 cut(s) 736
Hin1II CATG 5 cut(s) 397, 566, 629, 726, 848
HinfI GANTC 2 cut(s) 5, 716
Hpy166II GTNNAC 2 cut(s) 188, 427
Hpy188I TCNGA 3 cut(s) 153, 811, 939
Hpy188III TCNNGA 2 cut(s) 236, 408
Hpy8I GTNNAC 2 cut(s) 188, 427
HpyAV CCTTC 3 cut(s) 257, 701, 766
HpyCH4III ACNGT 1 cut(s) 634
HpyCH4IV ACGT 1 cut(s) 110
HpyCH4V TGCA 6 cut(s) 29, 125, 397, 629, 887, 953
HpyF10VI GCNNNNNNNGC 5 cut(s) 215, 559, 773, 959, 1037
HpyF3I CTNAG 3 cut(s) 82, 348, 908
HpySE526I ACGT 1 cut(s) 110
Hsp92II CATG 5 cut(s) 397, 566, 629, 726, 848
Ksp22I TGATCA 1 cut(s) 298
Kzo9I GATC 3 cut(s) 280, 298, 373
LmnI GCTCC 1 cut(s) 526
Lsp1109I GCAGC 4 cut(s) 163, 298, 739, 812
LweI GCATC 4 cut(s) 124, 134, 406, 874
MaeI CTAG 4 cut(s) 365, 603, 669, 1054
MaeII ACGT 1 cut(s) 110
MalI GATC 3 cut(s) 282, 300, 375
MboI GATC 3 cut(s) 280, 298, 373
MboII GAAGA 2 cut(s) 251, 364
MfeI CAATTG 1 cut(s) 963
MlyI GAGTC 1 cut(s) 725
MnlI CCTC 9 cut(s) 37, 80, 516, 524, 726, 850, 903, 937, 1001
Mph1103I ATGCAT 1 cut(s) 399
MseI TTAA 2 cut(s) 53, 903
MspR9I CCNGG 1 cut(s) 262
MunI CAATTG 1 cut(s) 963
MvaI CCWGG 1 cut(s) 262
MwoI GCNNNNNNNGC 5 cut(s) 215, 559, 773, 959, 1037
NdeI CATATG 1 cut(s) 547
NdeII GATC 3 cut(s) 280, 298, 373
NheI GCTAGC 1 cut(s) 668
NlaIII CATG 5 cut(s) 397, 566, 629, 726, 848
NlaIV GGNNCC 1 cut(s) 984
NsiI ATGCAT 1 cut(s) 399
NspI RCATGY 2 cut(s) 566, 848
PceI AGGCCT 1 cut(s) 736
PfeI GAWTC 1 cut(s) 5
PkrI GCNGC 4 cut(s) 178, 313, 754, 802
PleI GAGTC 1 cut(s) 724
PpsI GAGTC 1 cut(s) 724
Psp1406I AACGTT 1 cut(s) 110
Psp6I CCWGG 1 cut(s) 260
PspGI CCWGG 1 cut(s) 260
PspN4I GGNNCC 1 cut(s) 984
PsrI GAACNNNNNNTAC 2 cut(s) 370, 402
RsaI GTAC 1 cut(s) 478
RsaNI GTAC 1 cut(s) 477
SaqAI TTAA 2 cut(s) 53, 903
SatI GCNGC 4 cut(s) 177, 312, 753, 801
Sau3AI GATC 3 cut(s) 280, 298, 373
SchI GAGTC 1 cut(s) 725
ScrFI CCNGG 1 cut(s) 262
SfaNI GCATC 4 cut(s) 124, 134, 406, 874
SfcI CTRYAG 2 cut(s) 145, 738
SmlI CTYRAG 1 cut(s) 12
SmoI CTYRAG 1 cut(s) 12
SseBI AGGCCT 1 cut(s) 736
SsiI CCGC 1 cut(s) 991
SspI AATATT 1 cut(s) 1001
SspMI CTAG 4 cut(s) 365, 603, 669, 1054
StuI AGGCCT 1 cut(s) 736
StyD4I CCNGG 1 cut(s) 260
StyI CCWWGG 1 cut(s) 762
TaaI ACNGT 1 cut(s) 634
TaiI ACGT 1 cut(s) 113
TfiI GAWTC 1 cut(s) 5
Tru1I TTAA 2 cut(s) 53, 903
Tru9I TTAA 2 cut(s) 53, 903
TseI GCWGC 4 cut(s) 176, 311, 752, 800
XagI CCTNNNNNAGG 1 cut(s) 766
XapI RAATTY 5 cut(s) 223, 327, 411, 431, 927
XceI RCATGY 2 cut(s) 566, 848
XspI CTAG 4 cut(s) 365, 603, 669, 1054
Zsp2I ATGCAT 1 cut(s) 399
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.