Rw3G014870

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0002165
Physical Location & Seq
Forward (+)
15035367 .. 15035867
501 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G014870.1

Sequence Viewer

Length: 228 bp
ATGGATCGGATCCGCTGCCTTCCCTTCTCTGTGCTGACCTTGTTCAATCCCTACATGTGGCAGCTTTCCAATCGGTTCAATTGGCATGACAAGGCAATGCTGTTTGAGCAGCATCACTGGAAAAAGGCACTGAAGAAAGGGCAGCCCTATCAATTTAAGTGGAATGAGTACATGGACAAGGACCTCAGAGATGCCTACTATATCAACTGGCCGGTTTATTTCCCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

75

Amino Acids

9.54

Weight (kDa)

9.34

Isoelectric Point (pI)

35.49

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 13
AclWI GGATC 3 cut(s) 4, 12, 17
AcoI YGGCCR 1 cut(s) 209
AcuI CTGAAG 1 cut(s) 152
AfaI GTAC 1 cut(s) 170
AfiI CCNNNNNNNGG 1 cut(s) 57
AflIII ACRYGT 1 cut(s) 54
AgsI TTSAA 2 cut(s) 46, 79
AluBI AGCT 1 cut(s) 64
AluI AGCT 1 cut(s) 64
AlwI GGATC 3 cut(s) 4, 12, 17
AoxI GGCC 1 cut(s) 209
ApeKI GCWGC 4 cut(s) 15, 61, 109, 142
AspS9I GGNCC 1 cut(s) 181
AvaII GGWCC 1 cut(s) 181
BamHI GGATCC 1 cut(s) 9
BbvI GCAGC 4 cut(s) 2, 73, 121, 154
BisI GCNGC 4 cut(s) 16, 62, 110, 143
BlsI GCNGC 4 cut(s) 17, 63, 111, 144
Bme18I GGWCC 1 cut(s) 181
BmgT120I GGNCC 1 cut(s) 181
BmiI GGNNCC 1 cut(s) 11
BmsI GCATC 2 cut(s) 121, 181
Bsc4I CCNNNNNNNGG 1 cut(s) 57
Bse118I RCCGGY 1 cut(s) 211
Bse1I ACTGG 2 cut(s) 122, 212
Bse3DI GCAATG 1 cut(s) 102
BseLI CCNNNNNNNGG 1 cut(s) 57
BseMI GCAATG 1 cut(s) 102
BseMII CTCAG 1 cut(s) 199
BseNI ACTGG 2 cut(s) 122, 212
BseXI GCAGC 4 cut(s) 2, 73, 121, 154
BshFI GGCC 1 cut(s) 211
BsiSI CCGG 1 cut(s) 212
BslI CCNNNNNNNGG 1 cut(s) 57
BsnI GGCC 1 cut(s) 211
Bsp143I GATC 2 cut(s) 4, 9
BspACI CCGC 1 cut(s) 13
BspANI GGCC 1 cut(s) 211
BspCNI CTCAG 1 cut(s) 198
BspLI GGNNCC 1 cut(s) 11
BspPI GGATC 3 cut(s) 4, 12, 17
BsrDI GCAATG 1 cut(s) 102
BsrFI RCCGGY 1 cut(s) 211
BsrI ACTGG 2 cut(s) 122, 212
BssAI RCCGGY 1 cut(s) 211
BssMI GATC 2 cut(s) 4, 9
BstDEI CTNAG 2 cut(s) 185, 225
BstKTI GATC 2 cut(s) 7, 12
BstMBI GATC 2 cut(s) 4, 9
BstMWI GCNNNNNNNGC 1 cut(s) 106
BstNSI RCATGY 1 cut(s) 58
BstV1I GCAGC 4 cut(s) 2, 73, 121, 154
BstX2I RGATCY 1 cut(s) 9
BstYI RGATCY 1 cut(s) 9
BsuRI GGCC 1 cut(s) 211
BtsIMutI CAGTG 2 cut(s) 115, 128
Cfr10I RCCGGY 1 cut(s) 211
Cfr13I GGNCC 1 cut(s) 181
Csp6I GTAC 1 cut(s) 169
CspCI CAANNNNNGTGG 2 cut(s) 140, 175
CviAII CATG 3 cut(s) 55, 86, 172
CviJI RGCY 3 cut(s) 64, 145, 211
CviKI_1 RGCY 3 cut(s) 64, 145, 211
CviQI GTAC 1 cut(s) 169
DdeI CTNAG 2 cut(s) 185, 225
DpnI GATC 2 cut(s) 6, 11
DpnII GATC 2 cut(s) 4, 9
EaeI YGGCCR 1 cut(s) 209
Eco47I GGWCC 1 cut(s) 181
Eco57I CTGAAG 1 cut(s) 152
EcoO109I RGGNCCY 1 cut(s) 181
FaeI CATG 3 cut(s) 58, 89, 175
FaiI YATR 4 cut(s) 56, 87, 173, 201
FatI CATG 3 cut(s) 54, 85, 171
Fnu4HI GCNGC 4 cut(s) 16, 62, 110, 143
Fsp4HI GCNGC 4 cut(s) 16, 62, 110, 143
GluI GCNGC 4 cut(s) 16, 62, 110, 143
HaeIII GGCC 1 cut(s) 211
HapII CCGG 1 cut(s) 212
Hin1II CATG 3 cut(s) 58, 89, 175
HpaII CCGG 1 cut(s) 212
Hpy188I TCNGA 2 cut(s) 9, 188
HpyAV CCTTC 2 cut(s) 29, 34
HpyF10VI GCNNNNNNNGC 1 cut(s) 106
HpyF3I CTNAG 2 cut(s) 185, 225
Hsp92II CATG 3 cut(s) 58, 89, 175
Kzo9I GATC 2 cut(s) 4, 9
LpnPI CCDG 2 cut(s) 103, 193
Lsp1109I GCAGC 4 cut(s) 2, 73, 121, 154
LweI GCATC 2 cut(s) 121, 181
MalI GATC 2 cut(s) 6, 11
MboI GATC 2 cut(s) 4, 9
MboII GAAGA 1 cut(s) 145
MfeI CAATTG 1 cut(s) 79
MflI RGATCY 1 cut(s) 9
MluCI AATT 2 cut(s) 79, 152
MnlI CCTC 1 cut(s) 194
MseI TTAA 1 cut(s) 156
MspA1I CMGCKG 1 cut(s) 15
MspI CCGG 1 cut(s) 212
MunI CAATTG 1 cut(s) 79
MwoI GCNNNNNNNGC 1 cut(s) 106
NdeII GATC 2 cut(s) 4, 9
NlaIII CATG 3 cut(s) 58, 89, 175
NlaIV GGNNCC 1 cut(s) 11
NspI RCATGY 1 cut(s) 58
PciI ACATGT 1 cut(s) 54
PkrI GCNGC 4 cut(s) 17, 63, 111, 144
PpuMI RGGWCCY 1 cut(s) 181
PscI ACATGT 1 cut(s) 54
Psp5II RGGWCCY 1 cut(s) 181
PspN4I GGNNCC 1 cut(s) 11
PspPI GGNCC 1 cut(s) 181
PspPPI RGGWCCY 1 cut(s) 181
PsuI RGATCY 1 cut(s) 9
RsaI GTAC 1 cut(s) 170
RsaNI GTAC 1 cut(s) 169
SaqAI TTAA 1 cut(s) 156
SatI GCNGC 4 cut(s) 16, 62, 110, 143
Sau3AI GATC 2 cut(s) 4, 9
Sau96I GGNCC 1 cut(s) 181
SetI ASST 3 cut(s) 41, 66, 186
SfaNI GCATC 2 cut(s) 121, 181
SgeI CNNG 9 cut(s) 52, 67, 98, 103, 130, 184, 190, 220, 224
SinI GGWCC 1 cut(s) 181
Sse9I AATT 2 cut(s) 79, 152
SsiI CCGC 1 cut(s) 13
TasI AATT 2 cut(s) 79, 152
TatI WGTACW 1 cut(s) 168
Tru1I TTAA 1 cut(s) 156
Tru9I TTAA 1 cut(s) 156
TscAI CASTG 2 cut(s) 122, 135
TseI GCWGC 4 cut(s) 15, 61, 109, 142
TspRI CASTG 2 cut(s) 122, 135
VpaK11BI GGWCC 1 cut(s) 181
XceI RCATGY 1 cut(s) 58
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.