MD13G1052000.v1.1

Protein of unknown function (DUF1262)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0002710
Physical Location & Seq
Reverse (-)
3694150 .. 3694639
490 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1052000.v1.1.491

Sequence Viewer

Length: 324 bp
ATGTATGTCACTAGGCCTCTGTCGATGTACAAAAAGTCTCCTGAGGATCTCTCATTACCCCCGCCAGAGGGTCCGAATTCTGGCATCTTGGTCATCCAAGATGAAGAAGCAGAGACTACATGTTGCTTTGGTTTATGCAAATCCCACGAGCTCCTTGACATGCCTTTACCTCAGAACAAGAATCTAAAGCTTCGTTATGTTACCCAGACGGGACAAAGCCAACATGTTTCTCATTTCTATACCGCCTTCATTCCGGTCCTTAACCAGCCCTTGGCTTCCAACCGTTACTACGCGATCCAGTCGCGTGGAAAGCTTGAAGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1262

Protein Analysis

108

Amino Acids

12.07

Weight (kDa)

6.81

Isoelectric Point (pI)

58.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1262 PF06880 23 - 107 1.9e-31 Protein of unknown function (DUF1262)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 271
AccII CGCG 2 cut(s) 293, 304
AciI CCGC 2 cut(s) 62, 243
AclWI GGATC 2 cut(s) 54, 289
AcsI RAATTY 1 cut(s) 76
AfaI GTAC 1 cut(s) 29
AfiI CCNNNNNNNGG 4 cut(s) 67, 68, 80, 271
AflIII ACRYGT 2 cut(s) 119, 223
AgsI TTSAA 1 cut(s) 317
AluBI AGCT 3 cut(s) 151, 190, 313
AluI AGCT 3 cut(s) 151, 190, 313
Alw21I GWGCWC 1 cut(s) 153
Alw26I GTCTC 2 cut(s) 42, 107
AlwI GGATC 2 cut(s) 54, 289
AoxI GGCC 1 cut(s) 14
ApoI RAATTY 1 cut(s) 76
AspS9I GGNCC 2 cut(s) 71, 256
AvaII GGWCC 2 cut(s) 71, 256
AxyI CCTNAGG 1 cut(s) 42
BanII GRGCYC 1 cut(s) 153
BauI CACGAG 1 cut(s) 146
Bbv12I GWGCWC 1 cut(s) 153
BcoDI GTCTC 2 cut(s) 42, 107
BfaI CTAG 1 cut(s) 12
Bme18I GGWCC 2 cut(s) 71, 256
BmgT120I GGNCC 2 cut(s) 71, 256
BmiI GGNNCC 1 cut(s) 72
BmsI GCATC 1 cut(s) 93
BplI GAGNNNNNCTC 2 cut(s) 35, 67
BsaJI CCNNGG 1 cut(s) 270
BsaWI WCCGGW 1 cut(s) 253
Bsc4I CCNNNNNNNGG 4 cut(s) 67, 68, 80, 271
Bse1I ACTGG 1 cut(s) 298
Bse21I CCTNAGG 1 cut(s) 42
BseDI CCNNGG 1 cut(s) 270
BseGI GGATG 1 cut(s) 93
BseLI CCNNNNNNNGG 4 cut(s) 67, 68, 80, 271
BseMII CTCAG 2 cut(s) 33, 185
BseNI ACTGG 1 cut(s) 298
Bsh1236I CGCG 2 cut(s) 293, 304
BshFI GGCC 1 cut(s) 16
BsiHKAI GWGCWC 1 cut(s) 153
BsiSI CCGG 1 cut(s) 254
BslFI GGGAC 1 cut(s) 225
BslI CCNNNNNNNGG 4 cut(s) 67, 68, 80, 271
BsmAI GTCTC 2 cut(s) 42, 107
BsmFI GGGAC 1 cut(s) 225
BsnI GGCC 1 cut(s) 16
Bsp1286I GDGCHC 1 cut(s) 153
Bsp1407I TGTACA 1 cut(s) 27
Bsp143I GATC 2 cut(s) 46, 294
BspACI CCGC 2 cut(s) 62, 243
BspANI GGCC 1 cut(s) 16
BspCNI CTCAG 2 cut(s) 34, 184
BspFNI CGCG 2 cut(s) 293, 304
BspLI GGNNCC 1 cut(s) 72
BspPI GGATC 2 cut(s) 54, 289
BsrGI TGTACA 1 cut(s) 27
BsrI ACTGG 1 cut(s) 298
BssECI CCNNGG 1 cut(s) 270
BssMI GATC 2 cut(s) 46, 294
BssSI CACGAG 1 cut(s) 146
BssT1I CCWWGG 1 cut(s) 270
Bst2BI CACGAG 1 cut(s) 146
Bst4CI ACNGT 1 cut(s) 284
BstAUI TGTACA 1 cut(s) 27
BstDEI CTNAG 2 cut(s) 42, 171
BstF5I GGATG 1 cut(s) 93
BstFNI CGCG 2 cut(s) 293, 304
BstKTI GATC 2 cut(s) 49, 297
BstMAI GTCTC 2 cut(s) 42, 107
BstMBI GATC 2 cut(s) 46, 294
BstMWI GCNNNNNNNGC 1 cut(s) 310
BstNSI RCATGY 3 cut(s) 123, 163, 227
BstUI CGCG 2 cut(s) 293, 304
BstX2I RGATCY 1 cut(s) 46
BstXI CCANNNNNNTGG 1 cut(s) 305
BstYI RGATCY 1 cut(s) 46
Bsu36I CCTNAGG 1 cut(s) 42
BsuRI GGCC 1 cut(s) 16
BtsCI GGATG 1 cut(s) 93
Cfr13I GGNCC 2 cut(s) 71, 256
Csp6I GTAC 1 cut(s) 28
CviAII CATG 3 cut(s) 120, 160, 224
CviJI RGCY 7 cut(s) 16, 151, 190, 219, 268, 275, 313
CviKI_1 RGCY 7 cut(s) 16, 151, 190, 219, 268, 275, 313
CviQI GTAC 1 cut(s) 28
DdeI CTNAG 2 cut(s) 42, 171
DpnI GATC 2 cut(s) 48, 296
DpnII GATC 2 cut(s) 46, 294
Ecl136II GAGCTC 1 cut(s) 151
Eco130I CCWWGG 1 cut(s) 270
Eco147I AGGCCT 1 cut(s) 16
Eco24I GRGCYC 1 cut(s) 153
Eco47I GGWCC 2 cut(s) 71, 256
Eco53kI GAGCTC 1 cut(s) 151
Eco81I CCTNAGG 1 cut(s) 42
EcoICRI GAGCTC 1 cut(s) 151
EcoRI GAATTC 1 cut(s) 76
EcoT14I CCWWGG 1 cut(s) 270
EcoT38I GRGCYC 1 cut(s) 153
ErhI CCWWGG 1 cut(s) 270
FaeI CATG 3 cut(s) 123, 163, 227
FaiI YATR 7 cut(s) 6, 121, 136, 161, 198, 225, 240
FaqI GGGAC 1 cut(s) 225
FatI CATG 3 cut(s) 119, 159, 223
FauI CCCGC 1 cut(s) 69
FokI GGATG 1 cut(s) 80
FriOI GRGCYC 1 cut(s) 153
FspBI CTAG 1 cut(s) 12
HaeIII GGCC 1 cut(s) 16
HapII CCGG 1 cut(s) 254
Hin1II CATG 3 cut(s) 123, 163, 227
HindIII AAGCTT 2 cut(s) 188, 311
HinfI GANTC 1 cut(s) 181
HpaII CCGG 1 cut(s) 254
Hpy188I TCNGA 2 cut(s) 75, 174
Hpy188III TCNNGA 1 cut(s) 41
HpyAV CCTTC 2 cut(s) 256, 311
HpyCH4III ACNGT 1 cut(s) 284
HpyCH4V TGCA 1 cut(s) 138
HpyF10VI GCNNNNNNNGC 1 cut(s) 310
HpyF3I CTNAG 2 cut(s) 42, 171
Hsp92II CATG 3 cut(s) 123, 163, 227
Kzo9I GATC 2 cut(s) 46, 294
LmnI GCTCC 1 cut(s) 156
LpnPI CCDG 7 cut(s) 54, 66, 78, 218, 267, 278, 311
LweI GCATC 1 cut(s) 93
MaeI CTAG 1 cut(s) 12
MaeIII GTNAC 3 cut(s) 7, 199, 284
MalI GATC 2 cut(s) 48, 296
MboI GATC 2 cut(s) 46, 294
MboII GAAGA 1 cut(s) 116
MflI RGATCY 1 cut(s) 46
MhlI GDGCHC 1 cut(s) 153
MluCI AATT 1 cut(s) 76
MmeI TCCRAC 1 cut(s) 303
MnlI CCTC 4 cut(s) 27, 37, 61, 180
MseI TTAA 1 cut(s) 261
MspI CCGG 1 cut(s) 254
MvnI CGCG 2 cut(s) 293, 304
MwoI GCNNNNNNNGC 1 cut(s) 310
NdeII GATC 2 cut(s) 46, 294
NlaIII CATG 3 cut(s) 123, 163, 227
NlaIV GGNNCC 1 cut(s) 72
NmuCI GTSAC 1 cut(s) 7
NspI RCATGY 3 cut(s) 123, 163, 227
PceI AGGCCT 1 cut(s) 16
PciI ACATGT 2 cut(s) 119, 223
PfeI GAWTC 1 cut(s) 181
PflMI CCANNNNNTGG 1 cut(s) 271
PscI ACATGT 2 cut(s) 119, 223
Psp124BI GAGCTC 1 cut(s) 153
PspN4I GGNNCC 1 cut(s) 72
PspPI GGNCC 2 cut(s) 71, 256
PsuI RGATCY 1 cut(s) 46
RsaI GTAC 1 cut(s) 29
RsaNI GTAC 1 cut(s) 28
SacI GAGCTC 1 cut(s) 153
SaqAI TTAA 1 cut(s) 261
Sau3AI GATC 2 cut(s) 46, 294
Sau96I GGNCC 2 cut(s) 71, 256
SduI GDGCHC 1 cut(s) 153
SetI ASST 4 cut(s) 153, 172, 192, 315
SfaNI GCATC 1 cut(s) 93
SinI GGWCC 2 cut(s) 71, 256
Sse9I AATT 1 cut(s) 76
SseBI AGGCCT 1 cut(s) 16
SsiI CCGC 2 cut(s) 62, 243
SspMI CTAG 1 cut(s) 12
SstI GAGCTC 1 cut(s) 153
StuI AGGCCT 1 cut(s) 16
StyI CCWWGG 1 cut(s) 270
TaaI ACNGT 1 cut(s) 284
TaqI TCGA 1 cut(s) 23
TasI AATT 1 cut(s) 76
TatI WGTACW 1 cut(s) 27
TfiI GAWTC 1 cut(s) 181
Tru1I TTAA 1 cut(s) 261
Tru9I TTAA 1 cut(s) 261
TseFI GTSAC 1 cut(s) 7
Tsp45I GTSAC 1 cut(s) 7
TspDTI ATGAA 2 cut(s) 117, 238
Van91I CCANNNNNTGG 1 cut(s) 271
VpaK11BI GGWCC 2 cut(s) 71, 256
XapI RAATTY 1 cut(s) 76
XceI RCATGY 3 cut(s) 123, 163, 227
XspI CTAG 1 cut(s) 12
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.