Rorug04G0295000

Protein of unknown function (DUF1262)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0002710
Physical Location & Seq
Forward (+)
46917561 .. 46918772
1212 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0295000.1

Sequence Viewer

Length: 1212 bp
ATGGACGCAAAAGCTAAGCCAATTGAAGCCAAAAGGCGTGCCCAGCAGGAGGCTCAATGTTCTACAAAAGCTGCTGCTAGAGCTGATCGGGGAAAGATACTTTCTCTTCCTGGTAGAGCTGTAAGACAGTCTTCGGAAAAGAAAGAAACTCCTGCAATCGCGTCTTCAGATTCAGTGGTTGCTTCTCATTGTACGCAGTTTGATGTTAAAAGCCAAGTAGACAAAATCACAGCTCAACATGAATACGGCTCTCGGCTAATTCCTGTTCATGTCAAGTCAACCATGCATCCTGCCATTTATGAGTTCGGAGTGAAGTATTTGGCTGGGGAAATATCTGGGGGTAATGCTAGCTGTATTGCAATGCTTAGAGCGTTTCAGGGAGCCATTAGAGACTACTCGACACCAACAGACAAAAATCTTGTCCAAGACTTACCAGCAAAAATTGGTAGCTATGTCTCATTTTTAACTCGGTGCAAGCCACTCTCTATTAGAATGGGAAATGCAATTAGGTTTCTAAAGAGTCATCTTTCCCAAGTGATGGGGTTAAGCCTTTCCGAGTCCGAAACAAAGTTCACACTCTGTCAAGAAATTGACTGTTTTATAAATGATAAGATCATTTTTGCTGATAAGGTGATAGCTAGTCATGCTGCTACAAATATTAGGGATGGGGATGTTCTTCTCACATATGGATATTCGAATGTCGTTGAGATGACCCTTTTACATGCACATGAACTTGGGAAACAGTTTCATGTTGTGATAGTAGAGTCACGCCCTCAGCAGGAAGGCCAATTGCTTCTTAGACTGCAGGCAAAAGGCCTGACTTGTACATATACTTGCACAAACGCCCTGTTTACTATCATGCATCAGGTAACAAGAGTATTTCTGGGAGCTGCCTCCGTGTGTTCTAATGGAACGGTGTATTCAGTAATTGGATCAACATGTGTCGCTATGGTTGCAAATGCATTTTGCTTTCCAGTCTTTATCTGCTGTGAATCTTATAAGTTTCATGAAAGGGCACAGCTCAATTCAATTTGCTCTAACAAACTAGGTGATCCAGATGATATTGCAAAGGTTTCTAGAAGTGATCTGAACTTAAAGTATGATGCTACTCCATCAGATTACATATCTATGATTGTAACAGATTATGGCATGGTTCTACCAACAAGCATGCCTGCGATTGTAGGGATATCGCAGAGAGCACTTGTGAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1262

Protein Analysis

403

Amino Acids

44.06

Weight (kDa)

8.66

Isoelectric Point (pI)

33.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
IF-2B PF01008 111 - 386 7.8e-66 Initiation factor 2 subunit family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 602, 999
AccB7I CCANNNNNTGG 1 cut(s) 538
AccI GTMKAC 1 cut(s) 219
AccII CGCG 1 cut(s) 161
AclWI GGATC 2 cut(s) 940, 1046
AcuI CTGAAG 1 cut(s) 150
AfaI GTAC 2 cut(s) 193, 826
AfiI CCNNNNNNNGG 3 cut(s) 49, 538, 778
AflIII ACRYGT 1 cut(s) 938
AgsI TTSAA 2 cut(s) 26, 1029
AjnI CCWGG 1 cut(s) 109
Alw21I GWGCWC 1 cut(s) 1201
Alw26I GTCTC 2 cut(s) 384, 460
AlwI GGATC 2 cut(s) 940, 1046
AoxI GGCC 2 cut(s) 784, 814
ApeKI GCWGC 4 cut(s) 71, 74, 647, 890
ArsI GACNNNNNNTTYG 2 cut(s) 405, 437
AsuHPI GGTGA 2 cut(s) 643, 1061
AsuII TTCGAA 1 cut(s) 695
AsuNHI GCTAGC 1 cut(s) 347
BaeGI GKGCMC 2 cut(s) 43, 1018
BbsI GAAGAC 2 cut(s) 123, 156
Bbv12I GWGCWC 1 cut(s) 1201
BbvCI CCTCAGC 1 cut(s) 774
BbvI GCAGC 4 cut(s) 58, 61, 634, 877
BccI CCATC 3 cut(s) 532, 659, 1120
BceAI ACGGC 1 cut(s) 262
BciT130I CCWGG 1 cut(s) 111
BcoDI GTCTC 2 cut(s) 384, 460
BfaI CTAG 6 cut(s) 78, 348, 639, 1046, 1077, 1210
BfmI CTRYAG 1 cut(s) 803
BisI GCNGC 4 cut(s) 72, 75, 648, 891
BlpI GCTNAGC 1 cut(s) 15
BlsI GCNGC 4 cut(s) 73, 76, 649, 892
Bme1390I CCNGG 1 cut(s) 111
BmiI GGNNCC 1 cut(s) 382
BmrFI CCNGG 1 cut(s) 111
BmsI GCATC 3 cut(s) 295, 871, 1093
BmtI GCTAGC 1 cut(s) 351
BpiI GAAGAC 2 cut(s) 123, 156
Bpu10I CCTNAGC 1 cut(s) 774
Bpu1102I GCTNAGC 1 cut(s) 15
Bpu14I TTCGAA 1 cut(s) 695
Bsc4I CCNNNNNNNGG 3 cut(s) 49, 538, 778
Bse1I ACTGG 1 cut(s) 974
Bse3DI GCAATG 1 cut(s) 366
BseBI CCWGG 1 cut(s) 111
BseGI GGATG 3 cut(s) 286, 670, 676
BseLI CCNNNNNNNGG 3 cut(s) 49, 538, 778
BseMI GCAATG 1 cut(s) 366
BseMII CTCAG 1 cut(s) 788
BseNI ACTGG 1 cut(s) 974
BseSI GKGCMC 2 cut(s) 43, 1018
BseXI GCAGC 4 cut(s) 58, 61, 634, 877
BseYI CCCAGC 2 cut(s) 42, 323
Bsh1236I CGCG 1 cut(s) 161
BshFI GGCC 2 cut(s) 786, 816
BsiHKAI GWGCWC 1 cut(s) 1201
BslI CCNNNNNNNGG 3 cut(s) 49, 538, 778
BsmAI GTCTC 2 cut(s) 384, 460
BsnI GGCC 2 cut(s) 786, 816
Bsp119I TTCGAA 1 cut(s) 695
Bsp1286I GDGCHC 3 cut(s) 43, 1018, 1201
Bsp1407I TGTACA 1 cut(s) 824
Bsp143I GATC 5 cut(s) 85, 612, 932, 1051, 1084
Bsp1720I GCTNAGC 1 cut(s) 15
BspANI GGCC 2 cut(s) 786, 816
BspCNI CTCAG 1 cut(s) 787
BspFNI CGCG 1 cut(s) 161
BspHI TCATGA 1 cut(s) 1006
BspLI GGNNCC 1 cut(s) 382
BspMAI CTGCAG 1 cut(s) 807
BspOI GCTAGC 1 cut(s) 351
BspPI GGATC 2 cut(s) 940, 1046
BspT104I TTCGAA 1 cut(s) 695
BsrDI GCAATG 1 cut(s) 366
BsrGI TGTACA 1 cut(s) 824
BsrI ACTGG 1 cut(s) 974
BssMI GATC 5 cut(s) 85, 612, 932, 1051, 1084
Bst2UI CCWGG 1 cut(s) 111
Bst4CI ACNGT 4 cut(s) 129, 596, 744, 916
Bst6I CTCTTC 1 cut(s) 111
BstAUI TGTACA 1 cut(s) 824
BstBI TTCGAA 1 cut(s) 695
BstC8I GCNNGC 6 cut(s) 39, 349, 476, 807, 1169, 1173
BstDEI CTNAG 4 cut(s) 15, 365, 774, 797
BstF5I GGATG 3 cut(s) 286, 670, 676
BstFNI CGCG 1 cut(s) 161
BstKTI GATC 5 cut(s) 88, 615, 935, 1054, 1087
BstMAI GTCTC 2 cut(s) 384, 460
BstMBI GATC 5 cut(s) 85, 612, 932, 1051, 1084
BstMWI GCNNNNNNNGC 4 cut(s) 43, 80, 644, 953
BstNI CCWGG 1 cut(s) 111
BstNSI RCATGY 3 cut(s) 725, 942, 1171
BstSCI CCNGG 1 cut(s) 109
BstSFI CTRYAG 1 cut(s) 803
BstSLI GKGCMC 2 cut(s) 43, 1018
BstUI CGCG 1 cut(s) 161
BstV1I GCAGC 4 cut(s) 58, 61, 634, 877
BstV2I GAAGAC 2 cut(s) 123, 156
BsuRI GGCC 2 cut(s) 786, 816
BtsCI GGATG 3 cut(s) 286, 670, 676
BtsIMutI CAGTG 1 cut(s) 180
Cac8I GCNNGC 6 cut(s) 39, 349, 476, 807, 1169, 1173
CciI TCATGA 1 cut(s) 1006
CseI GACGC 2 cut(s) 14, 150
Csp6I GTAC 2 cut(s) 192, 825
CviQI GTAC 2 cut(s) 192, 825
DdeI CTNAG 4 cut(s) 15, 365, 774, 797
DpnI GATC 5 cut(s) 87, 614, 934, 1053, 1086
DpnII GATC 5 cut(s) 85, 612, 932, 1051, 1084
Eam1104I CTCTTC 1 cut(s) 111
EarI CTCTTC 1 cut(s) 111
Eco147I AGGCCT 1 cut(s) 816
Eco32I GATATC 1 cut(s) 1188
Eco57I CTGAAG 1 cut(s) 150
EcoRII CCWGG 1 cut(s) 109
EcoRV GATATC 1 cut(s) 1188
EcoT22I ATGCAT 3 cut(s) 288, 864, 964
FalI AAGNNNNNCTT 2 cut(s) 115, 147
FauNDI CATATG 1 cut(s) 685
FblI GTMKAC 1 cut(s) 219
Fnu4HI GCNGC 4 cut(s) 72, 75, 648, 891
FokI GGATG 3 cut(s) 273, 677, 683
Fsp4HI GCNGC 4 cut(s) 72, 75, 648, 891
FspBI CTAG 6 cut(s) 78, 348, 639, 1046, 1077, 1210
GluI GCNGC 4 cut(s) 72, 75, 648, 891
GsaI CCCAGC 2 cut(s) 46, 327
HaeIII GGCC 2 cut(s) 786, 816
HgaI GACGC 2 cut(s) 14, 150
HincII GTYRAC 1 cut(s) 279
HindII GTYRAC 1 cut(s) 279
HinfI GANTC 5 cut(s) 170, 520, 557, 764, 992
HphI GGTGA 2 cut(s) 643, 1061
Hpy166II GTNNAC 5 cut(s) 220, 279, 573, 852, 1207
Hpy188I TCNGA 7 cut(s) 136, 169, 308, 556, 562, 1089, 1117
Hpy188III TCNNGA 4 cut(s) 584, 1007, 1055, 1077
Hpy8I GTNNAC 5 cut(s) 220, 279, 573, 852, 1207
HpyAV CCTTC 1 cut(s) 776
HpyCH4III ACNGT 4 cut(s) 129, 596, 744, 916
HpyF10VI GCNNNNNNNGC 4 cut(s) 43, 80, 644, 953
HpyF3I CTNAG 4 cut(s) 15, 365, 774, 797
Kzo9I GATC 5 cut(s) 85, 612, 932, 1051, 1084
LmnI GCTCC 2 cut(s) 380, 887
Lsp1109I GCAGC 4 cut(s) 58, 61, 634, 877
LweI GCATC 3 cut(s) 295, 871, 1093
MaeI CTAG 6 cut(s) 78, 348, 639, 1046, 1077, 1210
MaeIII GTNAC 3 cut(s) 765, 868, 1135
MalI GATC 5 cut(s) 87, 614, 934, 1053, 1086
MboI GATC 5 cut(s) 85, 612, 932, 1051, 1084
MboII GAAGA 4 cut(s) 98, 123, 156, 668
MfeI CAATTG 2 cut(s) 21, 788
MhlI GDGCHC 3 cut(s) 43, 1018, 1201
MluCI AATT 9 cut(s) 21, 258, 441, 504, 588, 788, 927, 1024, 1029
MlyI GAGTC 3 cut(s) 529, 566, 773
MnlI CCTC 3 cut(s) 43, 783, 904
Mph1103I ATGCAT 3 cut(s) 288, 864, 964
MseI TTAA 4 cut(s) 207, 464, 545, 1094
MslI CAYNNNNRTG 2 cut(s) 726, 1127
MspR9I CCNGG 1 cut(s) 111
MunI CAATTG 2 cut(s) 21, 788
MvaI CCWGG 1 cut(s) 111
MvnI CGCG 1 cut(s) 161
MwoI GCNNNNNNNGC 4 cut(s) 43, 80, 644, 953
NdeI CATATG 1 cut(s) 685
NdeII GATC 5 cut(s) 85, 612, 932, 1051, 1084
NheI GCTAGC 1 cut(s) 347
NlaIV GGNNCC 1 cut(s) 382
NmeAIII GCCGAG 1 cut(s) 232
NmuCI GTSAC 1 cut(s) 765
NsiI ATGCAT 3 cut(s) 288, 864, 964
NspI RCATGY 3 cut(s) 725, 942, 1171
NspV TTCGAA 1 cut(s) 695
PaeI GCATGC 1 cut(s) 1171
PagI TCATGA 1 cut(s) 1006
PceI AGGCCT 1 cut(s) 816
PciI ACATGT 1 cut(s) 938
PfeI GAWTC 2 cut(s) 170, 992
PflMI CCANNNNNTGG 1 cut(s) 538
PkrI GCNGC 4 cut(s) 73, 76, 649, 892
PleI GAGTC 3 cut(s) 528, 565, 772
PpsI GAGTC 3 cut(s) 528, 565, 772
PscI ACATGT 1 cut(s) 938
PsiI TTATAA 2 cut(s) 602, 999
Psp6I CCWGG 1 cut(s) 109
PspFI CCCAGC 2 cut(s) 42, 323
PspGI CCWGG 1 cut(s) 109
PspN4I GGNNCC 1 cut(s) 382
PstI CTGCAG 1 cut(s) 807
RsaI GTAC 2 cut(s) 193, 826
RsaNI GTAC 2 cut(s) 192, 825
RseI CAYNNNNRTG 2 cut(s) 726, 1127
SaqAI TTAA 4 cut(s) 207, 464, 545, 1094
SatI GCNGC 4 cut(s) 72, 75, 648, 891
Sau3AI GATC 5 cut(s) 85, 612, 932, 1051, 1084
SchI GAGTC 3 cut(s) 529, 566, 773
ScrFI CCNGG 1 cut(s) 111
SduI GDGCHC 3 cut(s) 43, 1018, 1201
SfaNI GCATC 3 cut(s) 295, 871, 1093
SfcI CTRYAG 1 cut(s) 803
SfuI TTCGAA 1 cut(s) 695
SmiMI CAYNNNNRTG 2 cut(s) 726, 1127
SphI GCATGC 1 cut(s) 1171
Sse9I AATT 9 cut(s) 21, 258, 441, 504, 588, 788, 927, 1024, 1029
SseBI AGGCCT 1 cut(s) 816
SspI AATATT 1 cut(s) 658
SspMI CTAG 6 cut(s) 78, 348, 639, 1046, 1077, 1210
StuI AGGCCT 1 cut(s) 816
StyD4I CCNGG 1 cut(s) 109
TaaI ACNGT 4 cut(s) 129, 596, 744, 916
TaqI TCGA 2 cut(s) 398, 695
TasI AATT 9 cut(s) 21, 258, 441, 504, 588, 788, 927, 1024, 1029
TatI WGTACW 1 cut(s) 824
TfiI GAWTC 2 cut(s) 170, 992
Tru1I TTAA 4 cut(s) 207, 464, 545, 1094
Tru9I TTAA 4 cut(s) 207, 464, 545, 1094
TscAI CASTG 1 cut(s) 180
TseFI GTSAC 1 cut(s) 765
TseI GCWGC 4 cut(s) 71, 74, 647, 890
Tsp45I GTSAC 1 cut(s) 765
TspDTI ATGAA 6 cut(s) 255, 257, 737, 744, 995, 1023
TspGWI ACGGA 1 cut(s) 886
TspRI CASTG 1 cut(s) 180
Van91I CCANNNNNTGG 1 cut(s) 538
XbaI TCTAGA 1 cut(s) 1076
XceI RCATGY 3 cut(s) 725, 942, 1171
XmiI GTMKAC 1 cut(s) 219
XspI CTAG 6 cut(s) 78, 348, 639, 1046, 1077, 1210
Zsp2I ATGCAT 3 cut(s) 288, 864, 964
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.