pycom16g05060

Protein of unknown function (DUF1262)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0002710
Physical Location & Seq
Forward (+)
3257956 .. 3259347
1392 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom16g05060.1

Sequence Viewer

Length: 1188 bp
ATGTATGTGACAAGGCGTCTGTCGATGTACAAAAAGTCTCCTGCTGATCTCTCACTGCCTCCGCCGGAGGGTCCAAACTCCGGCATTCTTGTCATCAAAGACGAAGAAACGACATGCTGTTTCGGGTTATCGAATATCGATACGATTAAAGAGCTGCCTCTCCCTCAGAACAAGAGCCTTGAGCTTTACTATGCCGTTGGGTTTTATCCCAACAGAGAGCTTCAGTTCCACAGTGTTTTCTTCATCCCAGTTCTTGGTCAGCCCCTGTCTTCCAACCGCTACTATGCAGTATATCCATTTGGGAAGGATAGAGGGGAAGGGTACACAAGCGCAAGCGAGTATTCCATGGACATGGAAACATGCTGCTTCGACAGCTGCACCAACGATGTACTACCGCAACGTTTTGATCCCAATAACATGTATCAGCAATACAAGATTAATCTTCACAAAAAGGTTATCAATATTTCAGGTGGTTTTGTGGCCAGATCAATGGCTAAAGATGGGTTCCCTCCGGAGGCATTGTGGAGGAGAGGGTGGAAACTCACAAGCTCAACATCGCCCGGATTTGAACTAGGTGATGCACCGGGCCTAGACACCGCCCTACGTGCCCGTCTCCCGGATTTTAATTTCCCACTAAAGAATGTGGTTGTAGGGAAATGGTACTGTCCATTCATGTTTGTTAAGGAAGGGACACCGCAAACGTTGATAGACATAAGGAACAGATCAATGTACTATGAAATGACACTTGAGCAGAGGTGGGAAGAAGTATTTGTGAAGGATCATAATTTTCAGTGTGGTGAAGGCAATGCTGCGGGTGTGGATTTTGTTGTGCAAAGCGAAGTGGTTATGGTTGGAGGGAGGGAAGGCAGGTGTGATGAGAAAAATGTGGGGGATGATGGGTTGGTGTTGTATAGGAGTTTGAACAATGTTGGGCGAGAGACAAGGGTAGGGGTGAGTGTAGCAATTTATGAGAGGATGAAGTGGGAGCAAGGGAGGGTTGGGTGGGTGGGGGGAGAAGAAGGGACAGTGAGAGTTGAGAGGGTTGAGGACTATGGAGGGGTGGGTGAGTGGAAGAAGTTGGGATTTTTTGTATTGGTTGAGAGGTTTGCTTTGAAGAGAATGGATGGGAACTTGGTTTTTAGTCATGATTTCAGGCACACTCATCAGATTAGGACTAAATGGGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1262

Protein Analysis

396

Amino Acids

45.14

Weight (kDa)

6.16

Isoelectric Point (pI)

43.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1262 PF06880 23 - 123 1.7e-30 Protein of unknown function (DUF1262)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 858
Acc36I ACCTGC 1 cut(s) 858
AccB7I CCANNNNNTGG 1 cut(s) 254
AccIII TCCGGA 1 cut(s) 511
AciI CCGC 6 cut(s) 62, 277, 395, 597, 695, 812
AclI AACGTT 2 cut(s) 400, 701
AclWI GGATC 2 cut(s) 401, 786
AcoI YGGCCR 1 cut(s) 480
AcuI CTGAAG 1 cut(s) 206
AcyI GRCGYC 1 cut(s) 16
AfaI GTAC 5 cut(s) 29, 323, 390, 662, 731
AfiI CCNNNNNNNGG 5 cut(s) 68, 80, 254, 514, 616
AflIII ACRYGT 1 cut(s) 417
AgsI TTSAA 3 cut(s) 569, 922, 1114
AhdI GACNNNNNGTC 1 cut(s) 15
AluBI AGCT 5 cut(s) 154, 184, 220, 375, 549
AluI AGCT 5 cut(s) 154, 184, 220, 375, 549
Alw26I GTCTC 3 cut(s) 42, 617, 932
AlwI GGATC 2 cut(s) 401, 786
AlwNI CAGNNNCTG 1 cut(s) 265
Aor13HI TCCGGA 1 cut(s) 511
AoxI GGCC 2 cut(s) 480, 586
ApeKI GCWGC 4 cut(s) 154, 363, 375, 809
AseI ATTAAT 1 cut(s) 438
AspLEI GCGC 1 cut(s) 332
AspS9I GGNCC 2 cut(s) 71, 586
AsuC2I CCSGG 3 cut(s) 561, 585, 617
AsuHPI GGTGA 4 cut(s) 587, 809, 964, 1076
AvaII GGWCC 1 cut(s) 71
BaeGI GKGCMC 1 cut(s) 610
BalI TGGCCA 1 cut(s) 482
BbsI GAAGAC 1 cut(s) 261
BbvI GCAGC 4 cut(s) 141, 350, 362, 796
BccI CCATC 3 cut(s) 494, 890, 1118
BceAI ACGGC 1 cut(s) 179
BcnI CCSGG 3 cut(s) 561, 585, 617
BcoDI GTCTC 3 cut(s) 42, 617, 932
BfaI CTAG 2 cut(s) 572, 590
BfuAI ACCTGC 1 cut(s) 858
BisI GCNGC 4 cut(s) 155, 364, 376, 810
BlsI GCNGC 4 cut(s) 156, 365, 377, 811
Bme1390I CCNGG 3 cut(s) 561, 585, 617
Bme18I GGWCC 1 cut(s) 71
BmeRI GACNNNNNGTC 1 cut(s) 15
BmgT120I GGNCC 2 cut(s) 71, 586
BmiI GGNNCC 2 cut(s) 72, 506
BmrFI CCNGG 3 cut(s) 561, 585, 617
BmrI ACTGGG 1 cut(s) 242
BmsI GCATC 1 cut(s) 568
BmuI ACTGGG 1 cut(s) 242
BpiI GAAGAC 1 cut(s) 261
BpuEI CTTGAG 2 cut(s) 200, 767
BpuMI CCSGG 3 cut(s) 561, 585, 617
Bsa29I ATCGAT 1 cut(s) 138
BsaAI YACGTR 1 cut(s) 605
BsaHI GRCGYC 1 cut(s) 16
BsaJI CCNNGG 1 cut(s) 345
BsaWI WCCGGW 1 cut(s) 511
BsaXI ACNNNNNCTCC 2 cut(s) 506, 536
Bsc4I CCNNNNNNNGG 5 cut(s) 68, 80, 254, 514, 616
Bse1I ACTGG 1 cut(s) 248
Bse3DI GCAATG 1 cut(s) 811
BseAI TCCGGA 1 cut(s) 511
BseCI ATCGAT 1 cut(s) 138
BseDI CCNNGG 1 cut(s) 345
BseGI GGATG 4 cut(s) 243, 898, 981, 1129
BseLI CCNNNNNNNGG 5 cut(s) 68, 80, 254, 514, 616
BseMI GCAATG 1 cut(s) 811
BseMII CTCAG 1 cut(s) 179
BseNI ACTGG 1 cut(s) 248
BseRI GAGGAG 1 cut(s) 541
BseSI GKGCMC 1 cut(s) 610
BseXI GCAGC 4 cut(s) 141, 350, 362, 796
BsgI GTGCAG 1 cut(s) 361
BshFI GGCC 2 cut(s) 482, 588
BshVI ATCGAT 1 cut(s) 138
BsiSI CCGG 6 cut(s) 65, 81, 512, 561, 584, 617
BslFI GGGAC 2 cut(s) 703, 1036
BslI CCNNNNNNNGG 5 cut(s) 68, 80, 254, 514, 616
BsmAI GTCTC 3 cut(s) 42, 617, 932
BsmBI CGTCTC 1 cut(s) 617
BsmFI GGGAC 2 cut(s) 703, 1036
BsmI GAATGC 1 cut(s) 84
BsnI GGCC 2 cut(s) 482, 588
Bsp1286I GDGCHC 1 cut(s) 610
Bsp13I TCCGGA 1 cut(s) 511
Bsp1407I TGTACA 1 cut(s) 27
Bsp143I GATC 5 cut(s) 46, 406, 485, 722, 778
Bsp19I CCATGG 1 cut(s) 345
BspACI CCGC 6 cut(s) 62, 277, 395, 597, 695, 812
BspANI GGCC 2 cut(s) 482, 588
BspCNI CTCAG 1 cut(s) 178
BspDI ATCGAT 1 cut(s) 138
BspEI TCCGGA 1 cut(s) 511
BspHI TCATGA 1 cut(s) 1144
BspLI GGNNCC 2 cut(s) 72, 506
BspMI ACCTGC 1 cut(s) 858
BspPI GGATC 2 cut(s) 401, 786
BsrDI GCAATG 1 cut(s) 811
BsrGI TGTACA 1 cut(s) 27
BsrI ACTGG 1 cut(s) 248
BssECI CCNNGG 1 cut(s) 345
BssMI GATC 5 cut(s) 46, 406, 485, 722, 778
BssNI GRCGYC 1 cut(s) 16
BssT1I CCWWGG 1 cut(s) 345
Bst4CI ACNGT 3 cut(s) 233, 665, 1027
Bst6I CTCTTC 1 cut(s) 1109
BstACI GRCGYC 1 cut(s) 16
BstAUI TGTACA 1 cut(s) 27
BstBAI YACGTR 1 cut(s) 605
BstC8I GCNNGC 1 cut(s) 334
BstDEI CTNAG 1 cut(s) 165
BstDSI CCRYGG 1 cut(s) 345
BstF5I GGATG 4 cut(s) 243, 898, 981, 1129
BstHHI GCGC 1 cut(s) 332
BstKTI GATC 5 cut(s) 49, 409, 488, 725, 781
BstMAI GTCTC 3 cut(s) 42, 617, 932
BstMBI GATC 5 cut(s) 46, 406, 485, 722, 778
BstMWI GCNNNNNNNGC 2 cut(s) 372, 605
BstNSI RCATGY 3 cut(s) 117, 363, 421
BstSCI CCNGG 3 cut(s) 559, 583, 615
BstSLI GKGCMC 1 cut(s) 610
BstV1I GCAGC 4 cut(s) 141, 350, 362, 796
BstV2I GAAGAC 1 cut(s) 261
BstXI CCANNNNNNTGG 2 cut(s) 352, 490
Bsu15I ATCGAT 1 cut(s) 138
BsuRI GGCC 2 cut(s) 482, 588
BsuTUI ATCGAT 1 cut(s) 138
BtgI CCRYGG 1 cut(s) 345
BtgZI GCGATG 1 cut(s) 540
BtsCI GGATG 4 cut(s) 243, 898, 981, 1129
BtsI GCAGTG 1 cut(s) 53
BtsIMutI CAGTG 4 cut(s) 53, 238, 797, 1032
BveI ACCTGC 1 cut(s) 858
Cac8I GCNNGC 1 cut(s) 334
CaiI CAGNNNCTG 1 cut(s) 265
CciI TCATGA 1 cut(s) 1144
CfoI GCGC 1 cut(s) 332
Cfr13I GGNCC 2 cut(s) 71, 586
ClaI ATCGAT 1 cut(s) 138
CseI GACGC 1 cut(s) 5
Csp6I GTAC 5 cut(s) 28, 322, 389, 661, 730
CviAII CATG 7 cut(s) 114, 346, 352, 360, 418, 673, 1145
CviQI GTAC 5 cut(s) 28, 322, 389, 661, 730
DdeI CTNAG 1 cut(s) 165
DpnI GATC 5 cut(s) 48, 408, 487, 724, 780
DpnII GATC 5 cut(s) 46, 406, 485, 722, 778
DriI GACNNNNNGTC 1 cut(s) 15
EaeI YGGCCR 1 cut(s) 480
Eam1104I CTCTTC 1 cut(s) 1109
Eam1105I GACNNNNNGTC 1 cut(s) 15
EarI CTCTTC 1 cut(s) 1109
EciI GGCGGA 1 cut(s) 51
Eco130I CCWWGG 1 cut(s) 345
Eco47I GGWCC 1 cut(s) 71
Eco57I CTGAAG 1 cut(s) 206
EcoT14I CCWWGG 1 cut(s) 345
ErhI CCWWGG 1 cut(s) 345
Esp3I CGTCTC 1 cut(s) 617
FaeI CATG 7 cut(s) 117, 349, 355, 363, 421, 676, 1148
FaqI GGGAC 2 cut(s) 703, 1036
FatI CATG 7 cut(s) 113, 345, 351, 359, 417, 672, 1144
FauI CCCGC 1 cut(s) 805
Fnu4HI GCNGC 4 cut(s) 155, 364, 376, 810
FokI GGATG 4 cut(s) 230, 905, 988, 1136
Fsp4HI GCNGC 4 cut(s) 155, 364, 376, 810
FspBI CTAG 2 cut(s) 572, 590
GlaI GCGC 1 cut(s) 331
GluI GCNGC 4 cut(s) 155, 364, 376, 810
HaeIII GGCC 2 cut(s) 482, 588
HapII CCGG 6 cut(s) 65, 81, 512, 561, 584, 617
HgaI GACGC 1 cut(s) 5
HhaI GCGC 1 cut(s) 332
Hin1I GRCGYC 1 cut(s) 16
Hin1II CATG 7 cut(s) 117, 349, 355, 363, 421, 676, 1148
Hin6I GCGC 1 cut(s) 330
HinP1I GCGC 1 cut(s) 330
HpaII CCGG 6 cut(s) 65, 81, 512, 561, 584, 617
HphI GGTGA 4 cut(s) 587, 809, 964, 1076
Hpy166II GTNNAC 1 cut(s) 324
Hpy188I TCNGA 2 cut(s) 168, 1167
Hpy188III TCNNGA 2 cut(s) 512, 1145
Hpy8I GTNNAC 1 cut(s) 324
HpyAV CCTTC 7 cut(s) 298, 311, 680, 769, 794, 857, 1013
HpyCH4III ACNGT 3 cut(s) 233, 665, 1027
HpyCH4IV ACGT 3 cut(s) 400, 604, 701
HpyCH4V TGCA 4 cut(s) 287, 378, 581, 832
HpyF10VI GCNNNNNNNGC 2 cut(s) 372, 605
HpyF3I CTNAG 1 cut(s) 165
HpySE526I ACGT 3 cut(s) 400, 604, 701
Hsp92I GRCGYC 1 cut(s) 16
Hsp92II CATG 7 cut(s) 117, 349, 355, 363, 421, 676, 1148
HspAI GCGC 1 cut(s) 330
Kpn2I TCCGGA 1 cut(s) 511
Kzo9I GATC 5 cut(s) 46, 406, 485, 722, 778
LmnI GCTCC 1 cut(s) 985
Lsp1109I GCAGC 4 cut(s) 141, 350, 362, 796
LweI GCATC 1 cut(s) 568
MaeI CTAG 2 cut(s) 572, 590
MaeII ACGT 3 cut(s) 400, 604, 701
MaeIII GTNAC 1 cut(s) 7
MalI GATC 5 cut(s) 48, 408, 487, 724, 780
MboI GATC 5 cut(s) 46, 406, 485, 722, 778
MboII GAAGA 8 cut(s) 116, 232, 261, 434, 773, 1028, 1084, 1126
MhlI GDGCHC 1 cut(s) 610
MlsI TGGCCA 1 cut(s) 482
MluCI AATT 3 cut(s) 625, 784, 963
MluNI TGGCCA 1 cut(s) 482
MmeI TCCRAC 2 cut(s) 297, 832
Mox20I TGGCCA 1 cut(s) 482
MroI TCCGGA 1 cut(s) 511
MscI TGGCCA 1 cut(s) 482
MseI TTAA 4 cut(s) 147, 438, 624, 681
MslI CAYNNNNRTG 1 cut(s) 350
Msp20I TGGCCA 1 cut(s) 482
MspA1I CMGCKG 1 cut(s) 375
MspI CCGG 6 cut(s) 65, 81, 512, 561, 584, 617
MspR9I CCNGG 3 cut(s) 561, 585, 617
Mva1269I GAATGC 1 cut(s) 84
MwoI GCNNNNNNNGC 2 cut(s) 372, 605
NciI CCSGG 3 cut(s) 561, 585, 617
NcoI CCATGG 1 cut(s) 345
NdeII GATC 5 cut(s) 46, 406, 485, 722, 778
NlaIII CATG 7 cut(s) 117, 349, 355, 363, 421, 676, 1148
NlaIV GGNNCC 2 cut(s) 72, 506
NmuCI GTSAC 1 cut(s) 7
NspI RCATGY 3 cut(s) 117, 363, 421
PagI TCATGA 1 cut(s) 1144
PaqCI CACCTGC 1 cut(s) 858
PciI ACATGT 1 cut(s) 417
PctI GAATGC 1 cut(s) 84
PflMI CCANNNNNTGG 1 cut(s) 254
PfoI TCCNGGA 1 cut(s) 615
PkrI GCNGC 4 cut(s) 156, 365, 377, 811
Ppu21I YACGTR 1 cut(s) 605
PscI ACATGT 1 cut(s) 417
PshBI ATTAAT 1 cut(s) 438
Psp1406I AACGTT 2 cut(s) 400, 701
PspN4I GGNNCC 2 cut(s) 72, 506
PspPI GGNCC 2 cut(s) 71, 586
PstNI CAGNNNCTG 1 cut(s) 265
PvuII CAGCTG 1 cut(s) 375
RsaI GTAC 5 cut(s) 29, 323, 390, 662, 731
RsaNI GTAC 5 cut(s) 28, 322, 389, 661, 730
RseI CAYNNNNRTG 1 cut(s) 350
SaqAI TTAA 4 cut(s) 147, 438, 624, 681
SatI GCNGC 4 cut(s) 155, 364, 376, 810
Sau3AI GATC 5 cut(s) 46, 406, 485, 722, 778
Sau96I GGNCC 2 cut(s) 71, 586
ScrFI CCNGG 3 cut(s) 561, 585, 617
SduI GDGCHC 1 cut(s) 610
SfaNI GCATC 1 cut(s) 568
SinI GGWCC 1 cut(s) 71
SmiMI CAYNNNNRTG 1 cut(s) 350
SmlI CTYRAG 2 cut(s) 179, 746
SmoI CTYRAG 2 cut(s) 179, 746
Sse9I AATT 3 cut(s) 625, 784, 963
SsiI CCGC 6 cut(s) 62, 277, 395, 597, 695, 812
SspI AATATT 1 cut(s) 463
SspMI CTAG 2 cut(s) 572, 590
StyD4I CCNGG 3 cut(s) 559, 583, 615
StyI CCWWGG 1 cut(s) 345
TaaI ACNGT 3 cut(s) 233, 665, 1027
TaiI ACGT 3 cut(s) 403, 607, 704
TaqI TCGA 4 cut(s) 23, 131, 138, 369
TasI AATT 3 cut(s) 625, 784, 963
TatI WGTACW 3 cut(s) 27, 388, 729
Tru1I TTAA 4 cut(s) 147, 438, 624, 681
Tru9I TTAA 4 cut(s) 147, 438, 624, 681
TscAI CASTG 4 cut(s) 60, 238, 797, 1032
TseFI GTSAC 1 cut(s) 7
TseI GCWGC 4 cut(s) 154, 363, 375, 809
Tsp45I GTSAC 1 cut(s) 7
TspDTI ATGAA 4 cut(s) 232, 661, 750, 992
TspRI CASTG 4 cut(s) 60, 238, 797, 1032
Van91I CCANNNNNTGG 1 cut(s) 254
VpaK11BI GGWCC 1 cut(s) 71
VspI ATTAAT 1 cut(s) 438
XceI RCATGY 3 cut(s) 117, 363, 421
XspI CTAG 2 cut(s) 572, 590
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.