MD16G1057800.v1.1

Protein of unknown function (DUF1262)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0002710
Physical Location & Seq
Forward (+)
4128891 .. 4130424
1534 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1057800.v1.1.491

Sequence Viewer

Length: 1191 bp
ATGTATGTGACAAGGCGTCTATCGATGTACAAAAGGTCTCCTGATGATCTCTCACTGCCTCCGCCGGAGGGTCCAAACTCCGGCATTCTTGTCATCAAAGACGAAGAAACGACATGCTGTTTCGGGTTGTCGAATATCGATATGATTAAAGAGCTGCCTCTCCCCCAGAACAAGAGCCTTGAGCTTTACTATGCCGTTGGGTTTTATCCCAACAGAGAGCTTCAGTTCCACAGTGTTTTCTTCATCCCAGTTCTTGGTCAGCCCCTGTCTTCCAACCGCTACTATGCAGTATATCCATTTGGGAAGGATAGAGGGGAAGGGTACACAAGCGCAAACGAGTATTCCATGGACATGGAAACATGCTGCTTCGACAGCTGCACCAAGGACGTACTACCGCAACGTTTTGATCCCAATAACATGTATCAGCAATACAAGATTAATCTTCACAAAAAAGTTATCAATATTTCAGGTGGTTTTGTGGCCAGATCAATGGCTAAAGATGGGTTCCCTCCACAGGCATTGTGGAGGAGAGGGTGGAAACTCAGAAGCTCAACATCGCCCGGATTTGAACTAGGTGATGCACCGGGCCTAGACACCGCCCTACGTGCCCGTCTCCCGGATTTTAATTTCCCACTAAAGAATGTGGTTGTAGGGAAATGGTACTGTCCATTCATGTTTGTTAACGAAGGGACACCGAAAACGTTGATAGACATAAGGAACAGATCAATGTTCTATGAGATGACACTTGAGCAGAGGTGGGAAGAAGTATTTGTGAAGGATCATAGTTTTCAATGCGGTGAAGGCAATGCTGCGCGTGTGGATTTTGTTGTGCAAAGTGAAGTGGTTATGGTTGGAGGGATGGAAGGCAGGTGTGATGGGAAAAAGGTGGGGGATGATGATGGGTTGGTGTTGTATAGGAGTTCGAACAATGTTGGGCAAGAGACAAGGGTGGGGGTGAGTGTAGCAATTTTTGAGAGGATGAAGTGGGAGCAAGGGAGGGTTGGGTGGGTGGGGGGAGAAGAAGGGACAGTGAGAGTTGAGAGGGTAGAGGACTACGGAGGGGTGGGTGAGTGGAAGAAATTAGGATTTTTTGTATTGGTTGAGAGGTTTGCTTTGAAGAGAATGAATGGGGACTTGGTTTTTAGTCATGATTTTAGGCACGCTCATCAGATTAGGACTAAATGGGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1262

Protein Analysis

397

Amino Acids

45.32

Weight (kDa)

6.63

Isoelectric Point (pI)

47.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1262 PF06880 23 - 123 2.1e-30 Protein of unknown function (DUF1262)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 858
Acc36I ACCTGC 1 cut(s) 858
AccB7I CCANNNNNTGG 1 cut(s) 254
AccII CGCG 1 cut(s) 814
AciI CCGC 5 cut(s) 62, 277, 395, 597, 795
AclI AACGTT 2 cut(s) 400, 701
AclWI GGATC 2 cut(s) 401, 786
AcoI YGGCCR 1 cut(s) 480
AcuI CTGAAG 1 cut(s) 206
AcyI GRCGYC 1 cut(s) 16
AfaI GTAC 4 cut(s) 29, 323, 390, 662
AfiI CCNNNNNNNGG 5 cut(s) 68, 80, 254, 514, 616
AflIII ACRYGT 1 cut(s) 417
AgsI TTSAA 3 cut(s) 569, 791, 1117
AhdI GACNNNNNGTC 1 cut(s) 15
AjuI GAANNNNNNNTTGG 2 cut(s) 1118, 1150
AluBI AGCT 5 cut(s) 154, 184, 220, 375, 549
AluI AGCT 5 cut(s) 154, 184, 220, 375, 549
Alw26I GTCTC 3 cut(s) 42, 617, 935
AlwI GGATC 2 cut(s) 401, 786
AlwNI CAGNNNCTG 1 cut(s) 265
AoxI GGCC 2 cut(s) 480, 586
ApeKI GCWGC 4 cut(s) 154, 363, 375, 809
AseI ATTAAT 1 cut(s) 438
AspLEI GCGC 2 cut(s) 332, 814
AspS9I GGNCC 2 cut(s) 71, 586
AsuC2I CCSGG 3 cut(s) 561, 585, 617
AsuHPI GGTGA 4 cut(s) 587, 809, 967, 1079
AsuII TTCGAA 1 cut(s) 923
AvaII GGWCC 1 cut(s) 71
BaeGI GKGCMC 1 cut(s) 610
BalI TGGCCA 1 cut(s) 482
BbsI GAAGAC 1 cut(s) 261
BbvI GCAGC 4 cut(s) 141, 350, 362, 796
BccI CCATC 4 cut(s) 494, 853, 869, 893
BceAI ACGGC 1 cut(s) 179
BcnI CCSGG 3 cut(s) 561, 585, 617
BcoDI GTCTC 3 cut(s) 42, 617, 935
BfaI CTAG 2 cut(s) 572, 590
BfuAI ACCTGC 1 cut(s) 858
BisI GCNGC 4 cut(s) 155, 364, 376, 810
BlsI GCNGC 4 cut(s) 156, 365, 377, 811
Bme1390I CCNGG 3 cut(s) 561, 585, 617
Bme18I GGWCC 1 cut(s) 71
BmeRI GACNNNNNGTC 1 cut(s) 15
BmgT120I GGNCC 2 cut(s) 71, 586
BmiI GGNNCC 2 cut(s) 72, 506
BmrFI CCNGG 3 cut(s) 561, 585, 617
BmrI ACTGGG 1 cut(s) 242
BmsI GCATC 1 cut(s) 568
BmuI ACTGGG 1 cut(s) 242
BpiI GAAGAC 1 cut(s) 261
Bpu14I TTCGAA 1 cut(s) 923
BpuEI CTTGAG 2 cut(s) 200, 767
BpuMI CCSGG 3 cut(s) 561, 585, 617
Bsa29I ATCGAT 2 cut(s) 23, 138
BsaAI YACGTR 1 cut(s) 605
BsaHI GRCGYC 1 cut(s) 16
BsaI GGTCTC 1 cut(s) 42
BsaJI CCNNGG 2 cut(s) 345, 381
Bsc4I CCNNNNNNNGG 5 cut(s) 68, 80, 254, 514, 616
Bse1I ACTGG 1 cut(s) 248
Bse3DI GCAATG 1 cut(s) 811
BseCI ATCGAT 2 cut(s) 23, 138
BseDI CCNNGG 2 cut(s) 345, 381
BseGI GGATG 4 cut(s) 243, 864, 898, 984
BseLI CCNNNNNNNGG 5 cut(s) 68, 80, 254, 514, 616
BseMI GCAATG 1 cut(s) 811
BseMII CTCAG 1 cut(s) 556
BseNI ACTGG 1 cut(s) 248
BseRI GAGGAG 1 cut(s) 541
BseSI GKGCMC 1 cut(s) 610
BseXI GCAGC 4 cut(s) 141, 350, 362, 796
BsgI GTGCAG 1 cut(s) 361
Bsh1236I CGCG 1 cut(s) 814
BshFI GGCC 2 cut(s) 482, 588
BshVI ATCGAT 2 cut(s) 23, 138
BsiSI CCGG 5 cut(s) 65, 81, 561, 584, 617
BslFI GGGAC 3 cut(s) 703, 1039, 1145
BslI CCNNNNNNNGG 5 cut(s) 68, 80, 254, 514, 616
BsmAI GTCTC 3 cut(s) 42, 617, 935
BsmBI CGTCTC 1 cut(s) 617
BsmFI GGGAC 3 cut(s) 703, 1039, 1145
BsmI GAATGC 1 cut(s) 84
BsnI GGCC 2 cut(s) 482, 588
Bso31I GGTCTC 1 cut(s) 42
Bsp119I TTCGAA 1 cut(s) 923
Bsp1286I GDGCHC 1 cut(s) 610
Bsp1407I TGTACA 1 cut(s) 27
Bsp143I GATC 5 cut(s) 46, 406, 485, 722, 778
Bsp19I CCATGG 1 cut(s) 345
BspACI CCGC 5 cut(s) 62, 277, 395, 597, 795
BspANI GGCC 2 cut(s) 482, 588
BspCNI CTCAG 1 cut(s) 555
BspDI ATCGAT 2 cut(s) 23, 138
BspFNI CGCG 1 cut(s) 814
BspHI TCATGA 1 cut(s) 1147
BspLI GGNNCC 2 cut(s) 72, 506
BspMI ACCTGC 1 cut(s) 858
BspPI GGATC 2 cut(s) 401, 786
BspT104I TTCGAA 1 cut(s) 923
BspTNI GGTCTC 1 cut(s) 42
BsrDI GCAATG 1 cut(s) 811
BsrGI TGTACA 1 cut(s) 27
BsrI ACTGG 1 cut(s) 248
BssECI CCNNGG 2 cut(s) 345, 381
BssMI GATC 5 cut(s) 46, 406, 485, 722, 778
BssNI GRCGYC 1 cut(s) 16
BssT1I CCWWGG 2 cut(s) 345, 381
Bst4CI ACNGT 3 cut(s) 233, 665, 1030
Bst6I CTCTTC 1 cut(s) 1112
BstACI GRCGYC 1 cut(s) 16
BstAUI TGTACA 1 cut(s) 27
BstBAI YACGTR 1 cut(s) 605
BstBI TTCGAA 1 cut(s) 923
BstC8I GCNNGC 1 cut(s) 1161
BstDEI CTNAG 1 cut(s) 542
BstDSI CCRYGG 1 cut(s) 345
BstF5I GGATG 4 cut(s) 243, 864, 898, 984
BstFNI CGCG 1 cut(s) 814
BstHHI GCGC 2 cut(s) 332, 814
BstKTI GATC 5 cut(s) 49, 409, 488, 725, 781
BstMAI GTCTC 3 cut(s) 42, 617, 935
BstMBI GATC 5 cut(s) 46, 406, 485, 722, 778
BstMWI GCNNNNNNNGC 3 cut(s) 372, 605, 801
BstNSI RCATGY 3 cut(s) 117, 363, 421
BstSCI CCNGG 3 cut(s) 559, 583, 615
BstSLI GKGCMC 1 cut(s) 610
BstUI CGCG 1 cut(s) 814
BstV1I GCAGC 4 cut(s) 141, 350, 362, 796
BstV2I GAAGAC 1 cut(s) 261
BstXI CCANNNNNNTGG 2 cut(s) 352, 490
Bsu15I ATCGAT 2 cut(s) 23, 138
BsuRI GGCC 2 cut(s) 482, 588
BsuTUI ATCGAT 2 cut(s) 23, 138
BtgI CCRYGG 1 cut(s) 345
BtgZI GCGATG 1 cut(s) 540
BtsCI GGATG 4 cut(s) 243, 864, 898, 984
BtsI GCAGTG 1 cut(s) 53
BtsIMutI CAGTG 3 cut(s) 53, 238, 1035
BveI ACCTGC 1 cut(s) 858
Cac8I GCNNGC 1 cut(s) 1161
CaiI CAGNNNCTG 1 cut(s) 265
CciI TCATGA 1 cut(s) 1147
CfoI GCGC 2 cut(s) 332, 814
Cfr13I GGNCC 2 cut(s) 71, 586
ClaI ATCGAT 2 cut(s) 23, 138
CseI GACGC 1 cut(s) 5
Csp6I GTAC 4 cut(s) 28, 322, 389, 661
CspCI CAANNNNNGTGG 2 cut(s) 501, 536
CviAII CATG 7 cut(s) 114, 346, 352, 360, 418, 673, 1148
CviQI GTAC 4 cut(s) 28, 322, 389, 661
DdeI CTNAG 1 cut(s) 542
DpnI GATC 5 cut(s) 48, 408, 487, 724, 780
DpnII GATC 5 cut(s) 46, 406, 485, 722, 778
DriI GACNNNNNGTC 1 cut(s) 15
EaeI YGGCCR 1 cut(s) 480
Eam1104I CTCTTC 1 cut(s) 1112
Eam1105I GACNNNNNGTC 1 cut(s) 15
EarI CTCTTC 1 cut(s) 1112
EciI GGCGGA 1 cut(s) 51
Eco130I CCWWGG 2 cut(s) 345, 381
Eco31I GGTCTC 1 cut(s) 42
Eco47I GGWCC 1 cut(s) 71
Eco57I CTGAAG 1 cut(s) 206
EcoT14I CCWWGG 2 cut(s) 345, 381
ErhI CCWWGG 2 cut(s) 345, 381
Esp3I CGTCTC 1 cut(s) 617
FaeI CATG 7 cut(s) 117, 349, 355, 363, 421, 676, 1151
FaqI GGGAC 3 cut(s) 703, 1039, 1145
FatI CATG 7 cut(s) 113, 345, 351, 359, 417, 672, 1147
Fnu4HI GCNGC 4 cut(s) 155, 364, 376, 810
FokI GGATG 4 cut(s) 230, 871, 905, 991
Fsp4HI GCNGC 4 cut(s) 155, 364, 376, 810
FspBI CTAG 2 cut(s) 572, 590
GlaI GCGC 2 cut(s) 331, 813
GluI GCNGC 4 cut(s) 155, 364, 376, 810
HaeIII GGCC 2 cut(s) 482, 588
HapII CCGG 5 cut(s) 65, 81, 561, 584, 617
HgaI GACGC 1 cut(s) 5
HhaI GCGC 2 cut(s) 332, 814
Hin1I GRCGYC 1 cut(s) 16
Hin1II CATG 7 cut(s) 117, 349, 355, 363, 421, 676, 1151
Hin6I GCGC 2 cut(s) 330, 812
HinP1I GCGC 2 cut(s) 330, 812
HincII GTYRAC 1 cut(s) 682
HindII GTYRAC 1 cut(s) 682
HpaI GTTAAC 1 cut(s) 682
HpaII CCGG 5 cut(s) 65, 81, 561, 584, 617
HphI GGTGA 4 cut(s) 587, 809, 967, 1079
Hpy166II GTNNAC 2 cut(s) 324, 682
Hpy188I TCNGA 2 cut(s) 545, 1170
Hpy188III TCNNGA 2 cut(s) 41, 1148
Hpy8I GTNNAC 2 cut(s) 324, 682
HpyAV CCTTC 7 cut(s) 298, 311, 680, 769, 794, 857, 1016
HpyCH4III ACNGT 3 cut(s) 233, 665, 1030
HpyCH4IV ACGT 4 cut(s) 387, 400, 604, 701
HpyCH4V TGCA 4 cut(s) 287, 378, 581, 832
HpyF10VI GCNNNNNNNGC 3 cut(s) 372, 605, 801
HpyF3I CTNAG 1 cut(s) 542
HpySE526I ACGT 4 cut(s) 387, 400, 604, 701
Hsp92I GRCGYC 1 cut(s) 16
Hsp92II CATG 7 cut(s) 117, 349, 355, 363, 421, 676, 1151
HspAI GCGC 2 cut(s) 330, 812
KspAI GTTAAC 1 cut(s) 682
Kzo9I GATC 5 cut(s) 46, 406, 485, 722, 778
LmnI GCTCC 1 cut(s) 988
Lsp1109I GCAGC 4 cut(s) 141, 350, 362, 796
LweI GCATC 1 cut(s) 568
MaeI CTAG 2 cut(s) 572, 590
MaeII ACGT 4 cut(s) 387, 400, 604, 701
MaeIII GTNAC 1 cut(s) 7
MalI GATC 5 cut(s) 48, 408, 487, 724, 780
MboI GATC 5 cut(s) 46, 406, 485, 722, 778
MboII GAAGA 8 cut(s) 116, 232, 261, 434, 773, 1031, 1087, 1129
MhlI GDGCHC 1 cut(s) 610
MlsI TGGCCA 1 cut(s) 482
MluCI AATT 3 cut(s) 625, 966, 1079
MluNI TGGCCA 1 cut(s) 482
MmeI TCCRAC 2 cut(s) 297, 832
Mox20I TGGCCA 1 cut(s) 482
MscI TGGCCA 1 cut(s) 482
MseI TTAA 4 cut(s) 147, 438, 624, 681
MslI CAYNNNNRTG 1 cut(s) 350
Msp20I TGGCCA 1 cut(s) 482
MspA1I CMGCKG 1 cut(s) 375
MspI CCGG 5 cut(s) 65, 81, 561, 584, 617
MspR9I CCNGG 3 cut(s) 561, 585, 617
Mva1269I GAATGC 1 cut(s) 84
MvnI CGCG 1 cut(s) 814
MwoI GCNNNNNNNGC 3 cut(s) 372, 605, 801
NciI CCSGG 3 cut(s) 561, 585, 617
NcoI CCATGG 1 cut(s) 345
NdeII GATC 5 cut(s) 46, 406, 485, 722, 778
NlaIII CATG 7 cut(s) 117, 349, 355, 363, 421, 676, 1151
NlaIV GGNNCC 2 cut(s) 72, 506
NmuCI GTSAC 1 cut(s) 7
NspI RCATGY 3 cut(s) 117, 363, 421
NspV TTCGAA 1 cut(s) 923
PagI TCATGA 1 cut(s) 1147
PaqCI CACCTGC 1 cut(s) 858
PciI ACATGT 1 cut(s) 417
PctI GAATGC 1 cut(s) 84
PflMI CCANNNNNTGG 1 cut(s) 254
PfoI TCCNGGA 1 cut(s) 615
PkrI GCNGC 4 cut(s) 156, 365, 377, 811
Ppu21I YACGTR 1 cut(s) 605
PscI ACATGT 1 cut(s) 417
PshBI ATTAAT 1 cut(s) 438
Psp1406I AACGTT 2 cut(s) 400, 701
PspN4I GGNNCC 2 cut(s) 72, 506
PspPI GGNCC 2 cut(s) 71, 586
PstNI CAGNNNCTG 1 cut(s) 265
PvuII CAGCTG 1 cut(s) 375
RsaI GTAC 4 cut(s) 29, 323, 390, 662
RsaNI GTAC 4 cut(s) 28, 322, 389, 661
RseI CAYNNNNRTG 1 cut(s) 350
SaqAI TTAA 4 cut(s) 147, 438, 624, 681
SatI GCNGC 4 cut(s) 155, 364, 376, 810
Sau3AI GATC 5 cut(s) 46, 406, 485, 722, 778
Sau96I GGNCC 2 cut(s) 71, 586
ScrFI CCNGG 3 cut(s) 561, 585, 617
SduI GDGCHC 1 cut(s) 610
SfaNI GCATC 1 cut(s) 568
SfuI TTCGAA 1 cut(s) 923
SinI GGWCC 1 cut(s) 71
SmiMI CAYNNNNRTG 1 cut(s) 350
SmlI CTYRAG 2 cut(s) 179, 746
SmoI CTYRAG 2 cut(s) 179, 746
Sse9I AATT 3 cut(s) 625, 966, 1079
SsiI CCGC 5 cut(s) 62, 277, 395, 597, 795
SspI AATATT 1 cut(s) 463
SspMI CTAG 2 cut(s) 572, 590
StyD4I CCNGG 3 cut(s) 559, 583, 615
StyI CCWWGG 2 cut(s) 345, 381
TaaI ACNGT 3 cut(s) 233, 665, 1030
TaiI ACGT 4 cut(s) 390, 403, 607, 704
TaqI TCGA 5 cut(s) 23, 131, 138, 369, 923
TasI AATT 3 cut(s) 625, 966, 1079
TatI WGTACW 1 cut(s) 27
Tru1I TTAA 4 cut(s) 147, 438, 624, 681
Tru9I TTAA 4 cut(s) 147, 438, 624, 681
TscAI CASTG 3 cut(s) 60, 238, 1035
TseFI GTSAC 1 cut(s) 7
TseI GCWGC 4 cut(s) 154, 363, 375, 809
Tsp45I GTSAC 1 cut(s) 7
TspDTI ATGAA 4 cut(s) 232, 661, 995, 1139
TspGWI ACGGA 1 cut(s) 1071
TspRI CASTG 3 cut(s) 60, 238, 1035
Van91I CCANNNNNTGG 1 cut(s) 254
VpaK11BI GGWCC 1 cut(s) 71
VspI ATTAAT 1 cut(s) 438
XceI RCATGY 3 cut(s) 117, 363, 421
XcmI CCANNNNNNNNNTGG 1 cut(s) 519
XspI CTAG 2 cut(s) 572, 590
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.