MD13G1180000.v1.1

Protein ABCI12, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0002836
Physical Location & Seq
Reverse (-)
15008118 .. 15011879
3762 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1180000.v1.1.491

Sequence Viewer

Length: 1143 bp
ATGAGCTTCGCTTACCGTGCCGTCACAACGGTAACATTCCCCTCTTTCCCGCTGAACCCTAATCCCAAAACCCTAATTGCCCCAAATTTCACTCACCCGAGCTCACTGAACAAAACCCTTATACAAGCAAAGCCGAGATTAAGCCTCCAAATTCGAGCTGCGGCGAGCGATAGCAGCCAAAGCCCGTGGTGGGTGAACGTTTTGCCGACCAAAGCTTTCGGAGCCGAGAAATTCCTCAGGCTGATTTCAGGCGCGACTGCTAGCCCCATCTGCCAGTACGTCTCGGAACCCACCACGTTCCTTCATTCTGTGGACCCCAGAATCAAATTGGTTTGGCTTTTGGCTCTAGTTGTTTTACCAGCAAGGTCACATATAATACTGCGCTTTGGATTAGTAGTATACTTGGGCGTTTTGTCAATATGGATTCTTCCGAGACACACATGGATGGATCAACTGGGAAGAGTGTCCTTGATTTCTGGAATTTTATTCATTATGCTGGGGCTGGGTTCAGATGGTGTACCACCGCTTGTTCAGTTGAGAACCCCACCTCCTGGAATGATGGGATTATCAAATCTTCCTGCATCCCTGGAAGGTTACTCATATTTAATTATGAAGCTAGGTCCAATACAGTTTACAAGGAAGGGGTTGTCTGTAGCGAGCACAGCTGCTTGCTTAACCTTTACTGTCTTTCAAAGTGCGAGCCTTTGCCTTACAACCACAACCCCTGAAGAACTTGCATTTGCTTTGCGGTGGTTTATGCTCCCTTTGACACATCTTGGTGTGCCAGTGGCTGAAATTATTCTTACCCTCATGCTTTCATTGAGGTTCATCAATCTGGTTTTTGATGAGGTTCGGAATGTTGCATTGGGGATTGTATCTCGTAGGATAAACTGGGAACAGCTGACAATGGTGGAAACAATCGATCTTTTATTTGCCTACTTCCGTCGGATCTTCACAAATATTTTTAGCCATGCAGAGCAGATTTCACAGGCAATGATTGTCAGAGGTTTTAGAGGGGACAGCAATGCTCATAAGATTTACTTGTTATCAGATCCATCCATCGGGACTGCAGATATCATTTCCCTGTTATGCCTGGTTGGTGTAATAGGTGCTTCTGTATGGTTTGGCTACTTTCTAGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CbiQ

Protein Analysis

381

Amino Acids

42.02

Weight (kDa)

9.69

Isoelectric Point (pI)

43.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CbiQ PF02361 99 - 342 3.5e-20 Cobalt transport protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 551
AccI GTMKAC 1 cut(s) 399
AccII CGCG 1 cut(s) 254
AciI CCGC 4 cut(s) 50, 161, 524, 748
AclI AACGTT 1 cut(s) 198
AclWI GGATC 3 cut(s) 456, 956, 1046
AcsI RAATTY 4 cut(s) 85, 150, 230, 480
AcuI CTGAAG 1 cut(s) 747
AfaI GTAC 2 cut(s) 278, 519
AfiI CCNNNNNNNGG 3 cut(s) 190, 551, 1061
AgsI TTSAA 1 cut(s) 692
AjnI CCWGG 3 cut(s) 550, 585, 1092
AjuI GAANNNNNNNTTGG 2 cut(s) 848, 880
AloI GAACNNNNNNTCC 2 cut(s) 532, 564
AluBI AGCT 7 cut(s) 6, 102, 158, 215, 616, 665, 901
AluI AGCT 7 cut(s) 6, 102, 158, 215, 616, 665, 901
Alw21I GWGCWC 2 cut(s) 104, 662
Alw26I GTCTC 2 cut(s) 286, 427
AlwI GGATC 3 cut(s) 456, 956, 1046
AlwNI CAGNNNCTG 1 cut(s) 791
Ama87I CYCGRG 1 cut(s) 97
ApeKI GCWGC 3 cut(s) 158, 174, 665
ApoI RAATTY 4 cut(s) 85, 150, 230, 480
Asp700I GAANNNNTTC 1 cut(s) 798
AspLEI GCGC 2 cut(s) 254, 384
AspS9I GGNCC 2 cut(s) 313, 620
AsuHPI GGTGA 2 cut(s) 86, 205
AsuNHI GCTAGC 1 cut(s) 260
AvaI CYCGRG 1 cut(s) 97
AvaII GGWCC 2 cut(s) 313, 620
AxyI CCTNAGG 1 cut(s) 236
BanII GRGCYC 1 cut(s) 104
Bbv12I GWGCWC 2 cut(s) 104, 662
BbvI GCAGC 3 cut(s) 145, 186, 652
BccI CCATC 6 cut(s) 275, 439, 506, 553, 1063, 1067
BceAI ACGGC 1 cut(s) 5
BciT130I CCWGG 3 cut(s) 552, 587, 1094
BcoDI GTCTC 2 cut(s) 286, 427
BfaI CTAG 4 cut(s) 261, 347, 617, 1136
BfmI CTRYAG 2 cut(s) 651, 1068
BisI GCNGC 4 cut(s) 159, 162, 175, 666
BlsI GCNGC 4 cut(s) 160, 163, 176, 667
Bme1390I CCNGG 3 cut(s) 552, 587, 1094
Bme18I GGWCC 2 cut(s) 313, 620
BmeT110I CYCGRG 1 cut(s) 97
BmgT120I GGNCC 2 cut(s) 313, 620
BmiI GGNNCC 3 cut(s) 223, 288, 315
BmrFI CCNGG 3 cut(s) 552, 587, 1094
BmrI ACTGGG 2 cut(s) 464, 901
BmsI GCATC 1 cut(s) 590
BmtI GCTAGC 1 cut(s) 264
BmuI ACTGGG 2 cut(s) 464, 901
Bsa29I ATCGAT 1 cut(s) 921
BsaJI CCNNGG 2 cut(s) 185, 585
BsaXI ACNNNNNCTCC 2 cut(s) 532, 562
Bsc4I CCNNNNNNNGG 3 cut(s) 190, 551, 1061
Bse1I ACTGG 4 cut(s) 274, 459, 785, 896
Bse21I CCTNAGG 1 cut(s) 236
Bse3DI GCAATG 2 cut(s) 999, 1030
BseBI CCWGG 3 cut(s) 552, 587, 1094
BseCI ATCGAT 1 cut(s) 921
BseDI CCNNGG 2 cut(s) 185, 585
BseGI GGATG 3 cut(s) 450, 581, 1055
BseLI CCNNNNNNNGG 3 cut(s) 190, 551, 1061
BseMI GCAATG 2 cut(s) 999, 1030
BseMII CTCAG 1 cut(s) 250
BseNI ACTGG 4 cut(s) 274, 459, 785, 896
BseXI GCAGC 3 cut(s) 145, 186, 652
BseYI CCCAGC 2 cut(s) 496, 502
Bsh1236I CGCG 1 cut(s) 254
BshVI ATCGAT 1 cut(s) 921
BsiHKAI GWGCWC 2 cut(s) 104, 662
BsiHKCI CYCGRG 1 cut(s) 97
BslFI GGGAC 2 cut(s) 1031, 1078
BslI CCNNNNNNNGG 3 cut(s) 190, 551, 1061
BsmAI GTCTC 2 cut(s) 286, 427
BsmBI CGTCTC 1 cut(s) 286
BsmFI GGGAC 2 cut(s) 1031, 1078
BsoBI CYCGRG 1 cut(s) 97
Bsp1286I GDGCHC 2 cut(s) 104, 662
Bsp143I GATC 4 cut(s) 448, 922, 948, 1051
BspACI CCGC 4 cut(s) 50, 161, 524, 748
BspCNI CTCAG 1 cut(s) 249
BspDI ATCGAT 1 cut(s) 921
BspFNI CGCG 1 cut(s) 254
BspLI GGNNCC 3 cut(s) 223, 288, 315
BspMAI CTGCAG 1 cut(s) 1072
BspOI GCTAGC 1 cut(s) 264
BspPI GGATC 3 cut(s) 456, 956, 1046
BsrDI GCAATG 2 cut(s) 999, 1030
BsrI ACTGG 4 cut(s) 274, 459, 785, 896
BssECI CCNNGG 2 cut(s) 185, 585
BssMI GATC 4 cut(s) 448, 922, 948, 1051
BssNAI GTATAC 1 cut(s) 400
Bst1107I GTATAC 1 cut(s) 400
Bst2UI CCWGG 3 cut(s) 552, 587, 1094
Bst4CI ACNGT 4 cut(s) 17, 31, 630, 685
Bst6I CTCTTC 1 cut(s) 454
BstC8I GCNNGC 5 cut(s) 166, 262, 658, 670, 700
BstDEI CTNAG 1 cut(s) 236
BstDSI CCRYGG 1 cut(s) 185
BstF5I GGATG 3 cut(s) 450, 581, 1055
BstFNI CGCG 1 cut(s) 254
BstHHI GCGC 2 cut(s) 254, 384
BstKTI GATC 4 cut(s) 451, 925, 951, 1054
BstMAI GTCTC 2 cut(s) 286, 427
BstMBI GATC 4 cut(s) 448, 922, 948, 1051
BstMWI GCNNNNNNNGC 6 cut(s) 17, 174, 180, 221, 270, 662
BstNI CCWGG 3 cut(s) 552, 587, 1094
BstSCI CCNGG 3 cut(s) 550, 585, 1092
BstSFI CTRYAG 2 cut(s) 651, 1068
BstUI CGCG 1 cut(s) 254
BstV1I GCAGC 3 cut(s) 145, 186, 652
BstX2I RGATCY 2 cut(s) 948, 1051
BstYI RGATCY 2 cut(s) 948, 1051
BstZ17I GTATAC 1 cut(s) 400
Bsu15I ATCGAT 1 cut(s) 921
Bsu36I CCTNAGG 1 cut(s) 236
BsuTUI ATCGAT 1 cut(s) 921
BtgI CCRYGG 1 cut(s) 185
BtsCI GGATG 3 cut(s) 450, 581, 1055
BtsIMutI CAGTG 2 cut(s) 104, 792
Cac8I GCNNGC 5 cut(s) 166, 262, 658, 670, 700
CaiI CAGNNNCTG 1 cut(s) 791
CfoI GCGC 2 cut(s) 254, 384
Cfr13I GGNCC 2 cut(s) 313, 620
ClaI ATCGAT 1 cut(s) 921
Csp6I GTAC 2 cut(s) 277, 518
CspCI CAANNNNNGTGG 2 cut(s) 167, 202
CviAII CATG 3 cut(s) 441, 811, 971
CviQI GTAC 2 cut(s) 277, 518
DdeI CTNAG 1 cut(s) 236
DpnI GATC 4 cut(s) 450, 924, 950, 1053
DpnII GATC 4 cut(s) 448, 922, 948, 1051
Eam1104I CTCTTC 1 cut(s) 454
EarI CTCTTC 1 cut(s) 454
Ecl136II GAGCTC 1 cut(s) 102
Eco24I GRGCYC 1 cut(s) 104
Eco32I GATATC 1 cut(s) 1075
Eco47I GGWCC 2 cut(s) 313, 620
Eco53kI GAGCTC 1 cut(s) 102
Eco57I CTGAAG 1 cut(s) 747
Eco81I CCTNAGG 1 cut(s) 236
Eco88I CYCGRG 1 cut(s) 97
EcoICRI GAGCTC 1 cut(s) 102
EcoRII CCWGG 3 cut(s) 550, 585, 1092
EcoRV GATATC 1 cut(s) 1075
EcoT38I GRGCYC 1 cut(s) 104
Esp3I CGTCTC 1 cut(s) 286
FaeI CATG 3 cut(s) 444, 814, 974
FalI AAGNNNNNCTT 2 cut(s) 1025, 1057
FaqI GGGAC 2 cut(s) 1031, 1078
FatI CATG 3 cut(s) 440, 810, 970
FauI CCCGC 1 cut(s) 57
FblI GTMKAC 1 cut(s) 399
Fnu4HI GCNGC 4 cut(s) 159, 162, 175, 666
FokI GGATG 3 cut(s) 457, 568, 1042
FriOI GRGCYC 1 cut(s) 104
Fsp4HI GCNGC 4 cut(s) 159, 162, 175, 666
FspBI CTAG 4 cut(s) 261, 347, 617, 1136
GlaI GCGC 2 cut(s) 253, 383
GluI GCNGC 4 cut(s) 159, 162, 175, 666
GsaI CCCAGC 2 cut(s) 500, 506
HhaI GCGC 2 cut(s) 254, 384
Hin1II CATG 3 cut(s) 444, 814, 974
Hin6I GCGC 2 cut(s) 252, 382
HinP1I GCGC 2 cut(s) 252, 382
HindIII AAGCTT 1 cut(s) 213
HinfI GANTC 2 cut(s) 321, 424
HphI GGTGA 2 cut(s) 86, 205
Hpy166II GTNNAC 5 cut(s) 196, 313, 400, 518, 633
Hpy188I TCNGA 9 cut(s) 221, 286, 432, 511, 855, 948, 1004, 1051, 1142
Hpy188III TCNNGA 2 cut(s) 477, 1063
Hpy8I GTNNAC 5 cut(s) 196, 313, 400, 518, 633
Hpy99I CGWCG 1 cut(s) 948
HpyAV CCTTC 3 cut(s) 311, 584, 634
HpyCH4III ACNGT 4 cut(s) 17, 31, 630, 685
HpyCH4IV ACGT 3 cut(s) 198, 279, 296
HpyCH4V TGCA 5 cut(s) 581, 737, 863, 974, 1070
HpyF10VI GCNNNNNNNGC 6 cut(s) 17, 174, 180, 221, 270, 662
HpyF3I CTNAG 1 cut(s) 236
HpySE526I ACGT 3 cut(s) 198, 279, 296
Hsp92II CATG 3 cut(s) 444, 814, 974
HspAI GCGC 2 cut(s) 252, 382
Kzo9I GATC 4 cut(s) 448, 922, 948, 1051
LmnI GCTCC 2 cut(s) 221, 765
Lsp1109I GCAGC 3 cut(s) 145, 186, 652
LweI GCATC 1 cut(s) 590
MaeI CTAG 4 cut(s) 261, 347, 617, 1136
MaeII ACGT 3 cut(s) 198, 279, 296
MaeIII GTNAC 4 cut(s) 22, 31, 366, 593
MalI GATC 4 cut(s) 450, 924, 950, 1053
MboI GATC 4 cut(s) 448, 922, 948, 1051
MboII GAAGA 5 cut(s) 419, 471, 566, 740, 943
MflI RGATCY 2 cut(s) 948, 1051
MhlI GDGCHC 2 cut(s) 104, 662
MluCI AATT 8 cut(s) 75, 85, 150, 230, 326, 480, 606, 795
MmeI TCCRAC 1 cut(s) 926
MnlI CCTC 9 cut(s) 52, 155, 245, 558, 816, 818, 841, 998, 1007
MroXI GAANNNNTTC 1 cut(s) 798
MseI TTAA 3 cut(s) 140, 605, 674
MslI CAYNNNNRTG 2 cut(s) 443, 777
MspA1I CMGCKG 3 cut(s) 52, 665, 901
MspR9I CCNGG 3 cut(s) 552, 587, 1094
MvaI CCWGG 3 cut(s) 552, 587, 1094
MvnI CGCG 1 cut(s) 254
MwoI GCNNNNNNNGC 6 cut(s) 17, 174, 180, 221, 270, 662
NdeII GATC 4 cut(s) 448, 922, 948, 1051
NheI GCTAGC 1 cut(s) 260
NlaIII CATG 3 cut(s) 444, 814, 974
NlaIV GGNNCC 3 cut(s) 223, 288, 315
NmeAIII GCCGAG 2 cut(s) 159, 250
NmuCI GTSAC 2 cut(s) 22, 366
PdmI GAANNNNTTC 1 cut(s) 798
PfeI GAWTC 2 cut(s) 321, 424
PflMI CCANNNNNTGG 1 cut(s) 551
PfoI TCCNGGA 1 cut(s) 550
PkrI GCNGC 4 cut(s) 160, 163, 176, 667
Psp124BI GAGCTC 1 cut(s) 104
Psp1406I AACGTT 1 cut(s) 198
Psp6I CCWGG 3 cut(s) 550, 585, 1092
PspFI CCCAGC 2 cut(s) 496, 502
PspGI CCWGG 3 cut(s) 550, 585, 1092
PspN4I GGNNCC 3 cut(s) 223, 288, 315
PspPI GGNCC 2 cut(s) 313, 620
PstI CTGCAG 1 cut(s) 1072
PstNI CAGNNNCTG 1 cut(s) 791
PsuI RGATCY 2 cut(s) 948, 1051
PvuII CAGCTG 2 cut(s) 665, 901
RsaI GTAC 2 cut(s) 278, 519
RsaNI GTAC 2 cut(s) 277, 518
RseI CAYNNNNRTG 2 cut(s) 443, 777
SacI GAGCTC 1 cut(s) 104
SaqAI TTAA 3 cut(s) 140, 605, 674
SatI GCNGC 4 cut(s) 159, 162, 175, 666
Sau3AI GATC 4 cut(s) 448, 922, 948, 1051
Sau96I GGNCC 2 cut(s) 313, 620
ScrFI CCNGG 3 cut(s) 552, 587, 1094
SduI GDGCHC 2 cut(s) 104, 662
SfaNI GCATC 1 cut(s) 590
SfcI CTRYAG 2 cut(s) 651, 1068
SinI GGWCC 2 cut(s) 313, 620
SmiMI CAYNNNNRTG 2 cut(s) 443, 777
Sse9I AATT 8 cut(s) 75, 85, 150, 230, 326, 480, 606, 795
SsiI CCGC 4 cut(s) 50, 161, 524, 748
SspI AATATT 1 cut(s) 961
SspMI CTAG 4 cut(s) 261, 347, 617, 1136
SstI GAGCTC 1 cut(s) 104
StyD4I CCNGG 3 cut(s) 550, 585, 1092
TaaI ACNGT 4 cut(s) 17, 31, 630, 685
TaiI ACGT 3 cut(s) 201, 282, 299
TaqI TCGA 2 cut(s) 154, 921
TasI AATT 8 cut(s) 75, 85, 150, 230, 326, 480, 606, 795
TauI GCSGC 1 cut(s) 164
TfiI GAWTC 2 cut(s) 321, 424
Tru1I TTAA 3 cut(s) 140, 605, 674
Tru9I TTAA 3 cut(s) 140, 605, 674
TscAI CASTG 2 cut(s) 111, 792
TseFI GTSAC 2 cut(s) 22, 366
TseI GCWGC 3 cut(s) 158, 174, 665
Tsp45I GTSAC 2 cut(s) 22, 366
TspDTI ATGAA 5 cut(s) 293, 478, 626, 807, 817
TspGWI ACGGA 1 cut(s) 932
TspRI CASTG 2 cut(s) 111, 792
Van91I CCANNNNNTGG 1 cut(s) 551
VpaK11BI GGWCC 2 cut(s) 313, 620
XapI RAATTY 4 cut(s) 85, 150, 230, 480
XcmI CCANNNNNNNNNTGG 1 cut(s) 325
XmiI GTMKAC 1 cut(s) 399
XmnI GAANNNNTTC 1 cut(s) 798
XspI CTAG 4 cut(s) 261, 347, 617, 1136
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.