MD14G1110900.v1.1
MYB Family

Transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0003850
Physical Location & Seq
Reverse (-)
17637613 .. 17639301
1689 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1110900.v1.1.491

Sequence Viewer

Length: 972 bp
ATGGGTCACCACTACTGCTGCAAACAACGAAAGGTTAAGAGAGGGCTTTGGTCCCCTGAGGAAGATGAAAAGCTCGTTAGATACATCACCACATATGGCCCTGGCTGTTGGAGAAAAGTTCCTGAGCAAGCTGGGCTTCAAAGGTGTGGTAAGAGCTGCCGCCTGAGATGGCTCAATTACTTGAGGCCTGATATCAAAAGAGGAGGGTTTACACCAGAGGAGGAGAAATTGATTGTCAGACTTAATGGGGTTGTAGGAAACAGATGGGCGCATATAGCAAAATGTCTGCCTGGAAGAACCGACAATGAGATAAAGAACTACTGGAATTCATGGATTAAAAAGAAGCTAGAAAAGCCTTCTTCACCACCAATCACATCTCCCTCTAATATTGATCATCAGCACAATAACCAAATGGGTTGCAGTTATCCAAACCAGCAAGAATTTTTGAGCCATAATTTCATTACAAAGCCTACAGCACCTAGGGAAATCCTATACCCTTCTCCATACCCTTTATTCATGTTTGGCGCTGATGAGATTACCATAAAAAACATTAATGTACAATCAGCCGATCAGGATTTTCCACTCGCTGTGTCAAATAATTCGAACAACAACGGAACATGGATTTTGGATCAGCAGCAGCATCATCAAGTTCAAGCCCTTCCCCCTTCTACAGCAGAATTTACAAACAATAATATGGATGCACAGTTTCTGCCATCCCTCCTAGGGTCCAACATTGGAAACAATAACATGGTTCCTCTTGTAGTGGAATCATGTGGAAATATTGATGAGGGAGGAGAGATTTCAATGGAGTGCTTGCAAAATACTACTCAGGAGCTGAACGCATTGGTTCATTCCCGCCAGGCTCAACAGTGTTCATCAAACAGCTTTCATTTTTGGGACATTGTTGAAGGACCATCTTCATCTAATTTGGGATCGGCTAATATATTATCTTCTTTTCCATCATATCAATAA

Protein Analysis

324

Amino Acids

36.53

Weight (kDa)

7.12

Isoelectric Point (pI)

62.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Myb_DNA-binding PF00249 14 - 61 3.9e-17 Myb-like DNA-binding domain
Myb_DNA-bind_6 PF13921 17 - 76 2.1e-13 Myb-like DNA-binding domain
Myb_DNA-binding PF00249 67 - 111 1.6e-14 Myb-like DNA-binding domain
Myb_DNA-bind_6 PF13921 70 - 117 3.7e-07 Myb-like DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 160, 856
AclWI GGATC 2 cut(s) 636, 940
AcsI RAATTY 3 cut(s) 325, 440, 677
AfaI GTAC 1 cut(s) 558
AfiI CCNNNNNNNGG 1 cut(s) 723
AgsI TTSAA 4 cut(s) 140, 653, 804, 908
AjnI CCWGG 3 cut(s) 100, 289, 858
AluBI AGCT 6 cut(s) 73, 131, 156, 346, 835, 885
AluI AGCT 6 cut(s) 73, 131, 156, 346, 835, 885
AlwI GGATC 2 cut(s) 636, 940
AlwNI CAGNNNCTG 2 cut(s) 709, 835
AoxI GGCC 2 cut(s) 97, 185
ApeKI GCWGC 4 cut(s) 18, 156, 634, 637
ApoI RAATTY 3 cut(s) 325, 440, 677
AseI ATTAAT 1 cut(s) 552
AspA2I CCTAGG 2 cut(s) 479, 721
AspLEI GCGC 2 cut(s) 271, 527
AspS9I GGNCC 4 cut(s) 51, 98, 726, 911
AsuHPI GGTGA 2 cut(s) 79, 354
AsuII TTCGAA 1 cut(s) 602
AvaII GGWCC 3 cut(s) 51, 726, 911
AvrII CCTAGG 2 cut(s) 479, 721
AxyI CCTNAGG 1 cut(s) 57
BbvI GCAGC 4 cut(s) 5, 143, 646, 649
BccI CCATC 5 cut(s) 162, 258, 721, 922, 967
BciT130I CCWGG 3 cut(s) 102, 291, 860
BclI TGATCA 1 cut(s) 391
BfaI CTAG 3 cut(s) 347, 480, 722
BfmI CTRYAG 2 cut(s) 471, 669
BfoI RGCGCY 1 cut(s) 528
BisI GCNGC 5 cut(s) 19, 157, 160, 635, 638
BlnI CCTAGG 2 cut(s) 479, 721
BlsI GCNGC 5 cut(s) 20, 158, 161, 636, 639
Bme1390I CCNGG 3 cut(s) 102, 291, 860
Bme18I GGWCC 3 cut(s) 51, 726, 911
BmgT120I GGNCC 4 cut(s) 51, 98, 726, 911
BmiI GGNNCC 3 cut(s) 53, 727, 753
BmrFI CCNGG 3 cut(s) 102, 291, 860
BmsI GCATC 2 cut(s) 649, 688
Bpu10I CCTNAGC 1 cut(s) 123
Bpu14I TTCGAA 1 cut(s) 602
BpuEI CTTGAG 1 cut(s) 202
BsaJI CCNNGG 3 cut(s) 100, 479, 721
Bsc4I CCNNNNNNNGG 1 cut(s) 723
Bse1I ACTGG 1 cut(s) 326
Bse21I CCTNAGG 1 cut(s) 57
BseBI CCWGG 3 cut(s) 102, 291, 860
BseDI CCNNGG 3 cut(s) 100, 479, 721
BseGI GGATG 2 cut(s) 703, 713
BseLI CCNNNNNNNGG 1 cut(s) 723
BseMII CTCAG 4 cut(s) 48, 114, 155, 842
BseNI ACTGG 1 cut(s) 326
BseRI GAGGAG 4 cut(s) 216, 233, 236, 807
BseXI GCAGC 4 cut(s) 5, 143, 646, 649
BseYI CCCAGC 1 cut(s) 131
BshFI GGCC 2 cut(s) 99, 187
BslFI GGGAC 2 cut(s) 37, 911
BslI CCNNNNNNNGG 1 cut(s) 723
BsmFI GGGAC 2 cut(s) 37, 911
BsnI GGCC 2 cut(s) 99, 187
Bsp119I TTCGAA 1 cut(s) 602
Bsp1407I TGTACA 1 cut(s) 556
Bsp143I GATC 4 cut(s) 391, 568, 628, 932
BspACI CCGC 2 cut(s) 160, 856
BspANI GGCC 2 cut(s) 99, 187
BspCNI CTCAG 4 cut(s) 49, 115, 156, 841
BspLI GGNNCC 3 cut(s) 53, 727, 753
BspPI GGATC 2 cut(s) 636, 940
BspT104I TTCGAA 1 cut(s) 602
BsrGI TGTACA 1 cut(s) 556
BsrI ACTGG 1 cut(s) 326
BssECI CCNNGG 3 cut(s) 100, 479, 721
BssMI GATC 4 cut(s) 391, 568, 628, 932
BssT1I CCWWGG 2 cut(s) 479, 721
Bst2UI CCWGG 3 cut(s) 102, 291, 860
Bst4CI ACNGT 2 cut(s) 705, 870
BstAUI TGTACA 1 cut(s) 556
BstBI TTCGAA 1 cut(s) 602
BstC8I GCNNGC 2 cut(s) 129, 815
BstDEI CTNAG 4 cut(s) 57, 123, 164, 828
BstEII GGTNACC 1 cut(s) 5
BstF5I GGATG 2 cut(s) 703, 713
BstH2I RGCGCY 1 cut(s) 528
BstHHI GCGC 2 cut(s) 271, 527
BstKTI GATC 4 cut(s) 394, 571, 631, 935
BstMBI GATC 4 cut(s) 391, 568, 628, 932
BstMWI GCNNNNNNNGC 3 cut(s) 133, 275, 352
BstNI CCWGG 3 cut(s) 102, 291, 860
BstPI GGTNACC 1 cut(s) 5
BstSCI CCNGG 3 cut(s) 100, 289, 858
BstSFI CTRYAG 2 cut(s) 471, 669
BstV1I GCAGC 4 cut(s) 5, 143, 646, 649
Bsu36I CCTNAGG 1 cut(s) 57
BsuRI GGCC 2 cut(s) 99, 187
BtsCI GGATG 2 cut(s) 703, 713
BtsIMutI CAGTG 1 cut(s) 875
Cac8I GCNNGC 2 cut(s) 129, 815
CaiI CAGNNNCTG 2 cut(s) 709, 835
CfoI GCGC 2 cut(s) 271, 527
Cfr13I GGNCC 4 cut(s) 51, 98, 726, 911
Csp6I GTAC 1 cut(s) 557
CviAII CATG 5 cut(s) 330, 517, 618, 748, 771
CviQI GTAC 1 cut(s) 557
DdeI CTNAG 4 cut(s) 57, 123, 164, 828
DpnI GATC 4 cut(s) 393, 570, 630, 934
DpnII GATC 4 cut(s) 391, 568, 628, 932
Eco130I CCWWGG 2 cut(s) 479, 721
Eco147I AGGCCT 1 cut(s) 187
Eco32I GATATC 1 cut(s) 193
Eco47I GGWCC 3 cut(s) 51, 726, 911
Eco81I CCTNAGG 1 cut(s) 57
Eco91I GGTNACC 1 cut(s) 5
EcoO65I GGTNACC 1 cut(s) 5
EcoRI GAATTC 1 cut(s) 325
EcoRII CCWGG 3 cut(s) 100, 289, 858
EcoRV GATATC 1 cut(s) 193
EcoT14I CCWWGG 2 cut(s) 479, 721
ErhI CCWWGG 2 cut(s) 479, 721
FaeI CATG 5 cut(s) 333, 520, 621, 751, 774
FalI AAGNNNNNCTT 2 cut(s) 120, 152
FaqI GGGAC 2 cut(s) 37, 911
FatI CATG 5 cut(s) 329, 516, 617, 747, 770
FauI CCCGC 1 cut(s) 863
FauNDI CATATG 1 cut(s) 94
FbaI TGATCA 1 cut(s) 391
Fnu4HI GCNGC 5 cut(s) 19, 157, 160, 635, 638
FokI GGATG 2 cut(s) 700, 710
Fsp4HI GCNGC 5 cut(s) 19, 157, 160, 635, 638
FspBI CTAG 3 cut(s) 347, 480, 722
GlaI GCGC 2 cut(s) 270, 526
GluI GCNGC 5 cut(s) 19, 157, 160, 635, 638
GsaI CCCAGC 1 cut(s) 135
HaeII RGCGCY 1 cut(s) 528
HaeIII GGCC 2 cut(s) 99, 187
HhaI GCGC 2 cut(s) 271, 527
Hin1II CATG 5 cut(s) 333, 520, 621, 751, 774
Hin6I GCGC 2 cut(s) 269, 525
HinP1I GCGC 2 cut(s) 269, 525
HinfI GANTC 1 cut(s) 767
HphI GGTGA 2 cut(s) 79, 354
Hpy166II GTNNAC 1 cut(s) 210
Hpy188I TCNGA 1 cut(s) 239
Hpy188III TCNNGA 3 cut(s) 122, 572, 830
Hpy8I GTNNAC 1 cut(s) 210
HpyAV CCTTC 5 cut(s) 366, 507, 668, 675, 902
HpyCH4III ACNGT 2 cut(s) 705, 870
HpyCH4V TGCA 4 cut(s) 21, 420, 701, 817
HpyF10VI GCNNNNNNNGC 3 cut(s) 133, 275, 352
HpyF3I CTNAG 4 cut(s) 57, 123, 164, 828
Hsp92II CATG 5 cut(s) 333, 520, 621, 751, 774
HspAI GCGC 2 cut(s) 269, 525
Ksp22I TGATCA 1 cut(s) 391
Kzo9I GATC 4 cut(s) 391, 568, 628, 932
LmnI GCTCC 1 cut(s) 832
Lsp1109I GCAGC 4 cut(s) 5, 143, 646, 649
LweI GCATC 2 cut(s) 649, 688
MaeI CTAG 3 cut(s) 347, 480, 722
MaeIII GTNAC 1 cut(s) 5
MalI GATC 4 cut(s) 393, 570, 630, 934
MboI GATC 4 cut(s) 391, 568, 628, 932
MboII GAAGA 5 cut(s) 74, 306, 351, 909, 942
MluCI AATT 8 cut(s) 175, 227, 325, 440, 454, 598, 677, 925
MmeI TCCRAC 2 cut(s) 89, 753
MseI TTAA 4 cut(s) 36, 243, 336, 552
MspR9I CCNGG 3 cut(s) 102, 291, 860
MvaI CCWGG 3 cut(s) 102, 291, 860
MwoI GCNNNNNNNGC 3 cut(s) 133, 275, 352
NdeI CATATG 1 cut(s) 94
NdeII GATC 4 cut(s) 391, 568, 628, 932
NlaIII CATG 5 cut(s) 333, 520, 621, 751, 774
NlaIV GGNNCC 3 cut(s) 53, 727, 753
NmuCI GTSAC 1 cut(s) 5
NspV TTCGAA 1 cut(s) 602
PceI AGGCCT 1 cut(s) 187
PfeI GAWTC 1 cut(s) 767
PkrI GCNGC 5 cut(s) 20, 158, 161, 636, 639
PshBI ATTAAT 1 cut(s) 552
Psp6I CCWGG 3 cut(s) 100, 289, 858
PspEI GGTNACC 1 cut(s) 5
PspFI CCCAGC 1 cut(s) 131
PspGI CCWGG 3 cut(s) 100, 289, 858
PspN4I GGNNCC 3 cut(s) 53, 727, 753
PspPI GGNCC 4 cut(s) 51, 98, 726, 911
PstNI CAGNNNCTG 2 cut(s) 709, 835
RsaI GTAC 1 cut(s) 558
RsaNI GTAC 1 cut(s) 557
SaqAI TTAA 4 cut(s) 36, 243, 336, 552
SatI GCNGC 5 cut(s) 19, 157, 160, 635, 638
Sau3AI GATC 4 cut(s) 391, 568, 628, 932
Sau96I GGNCC 4 cut(s) 51, 98, 726, 911
ScrFI CCNGG 3 cut(s) 102, 291, 860
SetI ASST 9 cut(s) 36, 75, 133, 146, 158, 348, 481, 837, 887
SfaNI GCATC 2 cut(s) 649, 688
SfcI CTRYAG 2 cut(s) 471, 669
SfuI TTCGAA 1 cut(s) 602
SinI GGWCC 3 cut(s) 51, 726, 911
SmlI CTYRAG 1 cut(s) 181
SmoI CTYRAG 1 cut(s) 181
Sse9I AATT 8 cut(s) 175, 227, 325, 440, 454, 598, 677, 925
SseBI AGGCCT 1 cut(s) 187
SsiI CCGC 2 cut(s) 160, 856
SspI AATATT 2 cut(s) 388, 781
SspMI CTAG 3 cut(s) 347, 480, 722
StuI AGGCCT 1 cut(s) 187
StyD4I CCNGG 3 cut(s) 100, 289, 858
StyI CCWWGG 2 cut(s) 479, 721
TaaI ACNGT 2 cut(s) 705, 870
TaqI TCGA 1 cut(s) 602
TasI AATT 8 cut(s) 175, 227, 325, 440, 454, 598, 677, 925
TatI WGTACW 1 cut(s) 556
TauI GCSGC 1 cut(s) 162
TfiI GAWTC 1 cut(s) 767
Tru1I TTAA 4 cut(s) 36, 243, 336, 552
Tru9I TTAA 4 cut(s) 36, 243, 336, 552
TscAI CASTG 1 cut(s) 875
TseFI GTSAC 1 cut(s) 5
TseI GCWGC 4 cut(s) 18, 156, 634, 637
Tsp45I GTSAC 1 cut(s) 5
TspDTI ATGAA 8 cut(s) 81, 318, 448, 505, 839, 864, 878, 909
TspGWI ACGGA 1 cut(s) 627
TspRI CASTG 1 cut(s) 875
VpaK11BI GGWCC 3 cut(s) 51, 726, 911
VspI ATTAAT 1 cut(s) 552
XapI RAATTY 3 cut(s) 325, 440, 677
XmaJI CCTAGG 2 cut(s) 479, 721
XspI CTAG 3 cut(s) 347, 480, 722
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.