Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0002571

Physical Location & Seq

Genomic Coordinates Reverse (-)

28153930 .. 28157004

Gene Structure 3075 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1190300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 855 bp

ATGGATCCTCCACTGATCAACGAGTCTTCCTCCTCAGTAGCTAACCCAGCATCCTACAGCCTGGCGGAGATTTGGCCCTTCAGCGGCGAGCCCGGTGGCAGCGGTGGCGGGTTGGGGCTCGGAGTGGTTAACTTGGGTCAGAGTTTGGGTGGGCTTGGAGATAGCTCTGTGAACAGAGATGGGTCGTTGGAGGAATCGACTGTGACTGAGCAAAGCGGCGGCGGCGGCGGTGGTGGAAGGAAGAGAAGGGATGTGAACTATGAGGACGAGTCTTCGAAGCGGGTTTCCACTCGTAGTGGCAATGGCTTGAAAGATTTGGGTAAGCGGATGAAATTAGCGGTATCCAGAAATGAAAATGACAATTTGAAGGCTGAAGTTGAAGAAAGTTCAGCTGGTGGCGACAGCAAGCCAGCTGAAGAAAGCACTAAACCTTCCGAGCCACCCAAGCAAGACTATATCCATGTGAGGGCAAGAAGGGGCCAAGCTACAGATAGCCACAGTCTAGCCGAGAGAGCTAGGAGAGAGAAGATCAGCGAAAGGATGAAAGTTCTCCAAGATTTGGTTCCCGGATGCAATAAGGTTATTGGGAAAGCACTCGTCCTTGATGAGATTATTAATTACATCCAATCACTGCAGCACCAGGTCGAGTTCCTTTCGATGAAGCTGGAAGCAGTCAATTCAAGGGTGAACATGAATCCCACAATTGAAGCGTTTCCTCCAAAAGATCTTGGTGCACAGCCATTTGATGCTGCGGCGTTGTTATTCAGCTCGCACACACCAAGGGAATACGCCCAAAGCTCACAGCCCGAATGGCTACATATGCAGGTTGGTGGTAGTTTCGATAGAGCAACGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0006355 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0043565 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0097159 GO:0140110 GO:1901363 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

HLH

Protein Analysis

285

Amino Acids

30.48

Weight (kDa)

5.53

Isoelectric Point (pI)

65.36

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HLH	PF00010	165 - 212	7e-09	Helix-loop-helix DNA-binding domain
bHLH_SAC51	PF23173	170 - 214	5.5e-08	Transcription factor SAC51 bHLH domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0002571)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G62610 AT5G62610
fragaria_vesca	FvH4_5g15930 FvH4_5g15930 FvH4_5g15930
malus_domestica	MD06G1184400.v1.1 MD14G1190300.v1.1
prunus_persica	Prupe.5G183200_v2.0.a1 Prupe.5G183200_v2.0.a1 Prupe.5G183200_v2.0.a1 Prupe.5G183200_v2.0.a1 Prupe.5G183200_v2.0.a1
pyrus_communis	pycom02g03530 pycom06g16360 pycom14g15800 pycom15g07880
rosa_chinensis	RchiOBHm_Chr3g0480001 RchiOBHm_Chr4g0403251 RchiOBHm_Chr4g0405511 RchiOBHm_Chr5g0004951 RchiOBHm_Chr7g0180121
rosa_laevigata	RLG00000005291 RLG00000033379
rosa_multiflora	Rmu_co8134850.1_g000001 Rmu_ssc0000119.1_g000035
rosa_roxburghii	Rroxscaffold_3G00273110 Rroxscaffold_5G00374410
rosa_rugosa	Rorug03G0058400 Rorug03G0171100 Rorug06G0430700
rosa_samantha	Rh1CG130200 Rh3AG295000 Rh3CG073100 Rh7AG031200 Rh7BG031100 Rh7DG031700 Rh7DG200500
rosa_wichuraiana	Rw0G012070 Rw4G009590 Rw7G002590

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	814
AccB7I	CCANNNNNTGG	1 cut(s)	559
AclWI	GGATC	1 cut(s)	12
AcuI	CTGAAG	3 cut(s)	64, 393, 435
AfiI	CCNNNNNNNGG	3 cut(s)	83, 466, 559
AgsI	TTSAA	5 cut(s)	310, 367, 380, 681, 707
AjnI	CCWGG	2 cut(s)	60, 639
AjuI	GAANNNNNNNTTGG	2 cut(s)	546, 578
AluBI	AGCT	9 cut(s)	41, 165, 392, 413, 485, 515, 664, 768, 798
AluI	AGCT	9 cut(s)	41, 165, 392, 413, 485, 515, 664, 768, 798
Alw21I	GWGCWC	1 cut(s)	736
Alw44I	GTGCAC	1 cut(s)	732
AlwI	GGATC	1 cut(s)	12
AoxI	GGCC	2 cut(s)	74, 478
ApaLI	GTGCAC	1 cut(s)	732
ApeKI	GCWGC	3 cut(s)	99, 634, 749
AseI	ATTAAT	1 cut(s)	615
Asp700I	GAANNNNTTC	1 cut(s)	711
AspS9I	GGNCC	2 cut(s)	75, 478
AsuC2I	CCSGG	2 cut(s)	93, 567
AsuHPI	GGTGA	1 cut(s)	697
AsuII	TTCGAA	1 cut(s)	275
BaeGI	GKGCMC	1 cut(s)	736
BamHI	GGATCC	1 cut(s)	4
BanII	GRGCYC	2 cut(s)	93, 120
BbsI	GAAGAC	2 cut(s)	18, 264
Bbv12I	GWGCWC	1 cut(s)	736
BbvI	GCAGC	3 cut(s)	111, 646, 736
BccI	CCATC	1 cut(s)	173
BciT130I	CCWGG	2 cut(s)	62, 641
BciVI	GTATCC	1 cut(s)	352
BclI	TGATCA	1 cut(s)	15
BcnI	CCSGG	2 cut(s)	93, 567
BfaI	CTAG	2 cut(s)	503, 516
BfmI	CTRYAG	3 cut(s)	55, 486, 632
BfuAI	ACCTGC	1 cut(s)	814
BfuI	GTATCC	1 cut(s)	352
BglI	GCCNNNNNGGC	1 cut(s)	811
BglII	AGATCT	1 cut(s)	724
BisI	GCNGC	9 cut(s)	85, 100, 217, 220, 223, 226, 635, 750, 753
BlsI	GCNGC	9 cut(s)	86, 101, 218, 221, 224, 227, 636, 751, 754
Bme1390I	CCNGG	4 cut(s)	62, 93, 567, 641
BmgT120I	GGNCC	2 cut(s)	75, 478
BmiI	GGNNCC	3 cut(s)	6, 479, 564
BmrFI	CCNGG	4 cut(s)	62, 93, 567, 641
BmsI	GCATC	3 cut(s)	59, 560, 736
BpiI	GAAGAC	2 cut(s)	18, 264
BplI	GAGNNNNNCTC	2 cut(s)	14, 46
Bpu14I	TTCGAA	1 cut(s)	275
BpuMI	CCSGG	2 cut(s)	93, 567
BsaJI	CCNNGG	1 cut(s)	779
Bsc4I	CCNNNNNNNGG	3 cut(s)	83, 466, 559
Bse3DI	GCAATG	1 cut(s)	307
BseBI	CCWGG	2 cut(s)	62, 641
BseDI	CCNNGG	1 cut(s)	779
BseGI	GGATG	6 cut(s)	50, 256, 333, 546, 575, 621
BseLI	CCNNNNNNNGG	3 cut(s)	83, 466, 559
BseMI	GCAATG	1 cut(s)	307
BseMII	CTCAG	2 cut(s)	48, 198
BseRI	GAGGAG	1 cut(s)	22
BseSI	GKGCMC	1 cut(s)	736
BseXI	GCAGC	3 cut(s)	111, 646, 736
BseYI	CCCAGC	1 cut(s)	46
BshFI	GGCC	2 cut(s)	76, 480
BsiHKAI	GWGCWC	1 cut(s)	736
BsiSI	CCGG	2 cut(s)	93, 567
BslI	CCNNNNNNNGG	3 cut(s)	83, 466, 559
BsnI	GGCC	2 cut(s)	76, 480
Bsp119I	TTCGAA	1 cut(s)	275
Bsp1286I	GDGCHC	3 cut(s)	93, 120, 736
Bsp143I	GATC	4 cut(s)	4, 15, 528, 724
BspANI	GGCC	2 cut(s)	76, 480
BspCNI	CTCAG	2 cut(s)	47, 199
BspLI	GGNNCC	3 cut(s)	6, 479, 564
BspMAI	CTGCAG	1 cut(s)	636
BspMI	ACCTGC	1 cut(s)	814
BspPI	GGATC	1 cut(s)	12
BspT104I	TTCGAA	1 cut(s)	275
BsrDI	GCAATG	1 cut(s)	307
BssECI	CCNNGG	1 cut(s)	779
BssMI	GATC	4 cut(s)	4, 15, 528, 724
BssT1I	CCWWGG	1 cut(s)	779
Bst2UI	CCWGG	2 cut(s)	62, 641
Bst4CI	ACNGT	2 cut(s)	202, 500
Bst6I	CTCTTC	1 cut(s)	236
BstBI	TTCGAA	1 cut(s)	275
BstC8I	GCNNGC	4 cut(s)	89, 407, 411, 770
BstDEI	CTNAG	2 cut(s)	34, 207
BstF5I	GGATG	6 cut(s)	50, 256, 333, 546, 575, 621
BstKTI	GATC	4 cut(s)	7, 18, 531, 727
BstMBI	GATC	4 cut(s)	4, 15, 528, 724
BstMWI	GCNNNNNNNGC	8 cut(s)	47, 105, 222, 225, 445, 512, 811, 820
BstNI	CCWGG	2 cut(s)	62, 641
BstSCI	CCNGG	4 cut(s)	60, 91, 565, 639
BstSFI	CTRYAG	3 cut(s)	55, 486, 632
BstSLI	GKGCMC	1 cut(s)	736
BstV1I	GCAGC	3 cut(s)	111, 646, 736
BstV2I	GAAGAC	2 cut(s)	18, 264
BstX2I	RGATCY	2 cut(s)	4, 724
BstYI	RGATCY	2 cut(s)	4, 724
BsuI	GTATCC	1 cut(s)	352
BsuRI	GGCC	2 cut(s)	76, 480
BtsCI	GGATG	6 cut(s)	50, 256, 333, 546, 575, 621
BtsI	GCAGTG	1 cut(s)	629
BtsIMutI	CAGTG	2 cut(s)	11, 629
BveI	ACCTGC	1 cut(s)	814
Cac8I	GCNNGC	4 cut(s)	89, 407, 411, 770
Cfr13I	GGNCC	2 cut(s)	75, 478
CsiI	ACCWGGT	1 cut(s)	639
CviAII	CATG	2 cut(s)	461, 691
DdeI	CTNAG	2 cut(s)	34, 207
DpnI	GATC	4 cut(s)	6, 17, 530, 726
DpnII	GATC	4 cut(s)	4, 15, 528, 724
Eam1104I	CTCTTC	1 cut(s)	236
EarI	CTCTTC	1 cut(s)	236
EciI	GGCGGA	1 cut(s)	80
Eco130I	CCWWGG	1 cut(s)	779
Eco24I	GRGCYC	2 cut(s)	93, 120
Eco57I	CTGAAG	3 cut(s)	64, 393, 435
EcoRII	CCWGG	2 cut(s)	60, 639
EcoT14I	CCWWGG	1 cut(s)	779
EcoT38I	GRGCYC	2 cut(s)	93, 120
ErhI	CCWWGG	1 cut(s)	779
FaeI	CATG	2 cut(s)	464, 694
FaiI	YATR	6 cut(s)	261, 456, 462, 692, 819, 821
FatI	CATG	2 cut(s)	460, 690
FauI	CCCGC	2 cut(s)	101, 273
FauNDI	CATATG	1 cut(s)	819
FbaI	TGATCA	1 cut(s)	15
Fnu4HI	GCNGC	9 cut(s)	85, 100, 217, 220, 223, 226, 635, 750, 753
FokI	GGATG	6 cut(s)	37, 263, 340, 553, 582, 608
FriOI	GRGCYC	2 cut(s)	93, 120
Fsp4HI	GCNGC	9 cut(s)	85, 100, 217, 220, 223, 226, 635, 750, 753
FspBI	CTAG	2 cut(s)	503, 516
GluI	GCNGC	9 cut(s)	85, 100, 217, 220, 223, 226, 635, 750, 753
GsaI	CCCAGC	1 cut(s)	50
HaeIII	GGCC	2 cut(s)	76, 480
HapII	CCGG	2 cut(s)	93, 567
Hin1II	CATG	2 cut(s)	464, 694
HincII	GTYRAC	1 cut(s)	130
HindII	GTYRAC	1 cut(s)	130
HinfI	GANTC	4 cut(s)	23, 194, 269, 694
HpaI	GTTAAC	1 cut(s)	130
HpaII	CCGG	2 cut(s)	93, 567
HphI	GGTGA	1 cut(s)	697
Hpy166II	GTNNAC	5 cut(s)	130, 172, 256, 688, 734
Hpy188I	TCNGA	3 cut(s)	122, 141, 436
Hpy188III	TCNNGA	1 cut(s)	345
Hpy8I	GTNNAC	5 cut(s)	130, 172, 256, 688, 734
HpyAV	CCTTC	6 cut(s)	88, 231, 240, 361, 441, 468
HpyCH4III	ACNGT	2 cut(s)	202, 500
HpyCH4IV	ACGT	1 cut(s)	851
HpyCH4V	TGCA	4 cut(s)	573, 634, 734, 823
HpyF10VI	GCNNNNNNNGC	8 cut(s)	47, 105, 222, 225, 445, 512, 811, 820
HpyF3I	CTNAG	2 cut(s)	34, 207
HpySE526I	ACGT	1 cut(s)	851
Hsp92II	CATG	2 cut(s)	464, 694
Ksp22I	TGATCA	1 cut(s)	15
KspAI	GTTAAC	1 cut(s)	130
Kzo9I	GATC	4 cut(s)	4, 15, 528, 724
Lsp1109I	GCAGC	3 cut(s)	111, 646, 736
LweI	GCATC	3 cut(s)	59, 560, 736
MabI	ACCWGGT	1 cut(s)	639
MaeI	CTAG	2 cut(s)	503, 516
MaeII	ACGT	1 cut(s)	851
MaeIII	GTNAC	1 cut(s)	202
MalI	GATC	4 cut(s)	6, 17, 530, 726
MboI	GATC	4 cut(s)	4, 15, 528, 724
MboII	GAAGA	6 cut(s)	18, 253, 264, 392, 428, 538
MfeI	CAATTG	1 cut(s)	702
MflI	RGATCY	2 cut(s)	4, 724
MhlI	GDGCHC	3 cut(s)	93, 120, 736
MluCI	AATT	5 cut(s)	332, 361, 616, 676, 702
MlyI	GAGTC	2 cut(s)	32, 278
MmeI	TCCRAC	1 cut(s)	168
MnlI	CCTC	7 cut(s)	18, 40, 43, 184, 256, 459, 726
MroXI	GAANNNNTTC	1 cut(s)	711
MseI	TTAA	2 cut(s)	129, 615
MspA1I	CMGCKG	4 cut(s)	84, 102, 392, 413
MspI	CCGG	2 cut(s)	93, 567
MspR9I	CCNGG	4 cut(s)	62, 93, 567, 641
MunI	CAATTG	1 cut(s)	702
MvaI	CCWGG	2 cut(s)	62, 641
MwoI	GCNNNNNNNGC	8 cut(s)	47, 105, 222, 225, 445, 512, 811, 820
NciI	CCSGG	2 cut(s)	93, 567
NdeI	CATATG	1 cut(s)	819
NdeII	GATC	4 cut(s)	4, 15, 528, 724
NlaIII	CATG	2 cut(s)	464, 694
NlaIV	GGNNCC	3 cut(s)	6, 479, 564
NmeAIII	GCCGAG	1 cut(s)	532
NmuCI	GTSAC	1 cut(s)	202
NspV	TTCGAA	1 cut(s)	275
PdmI	GAANNNNTTC	1 cut(s)	711
PfeI	GAWTC	2 cut(s)	194, 694
PflMI	CCANNNNNTGG	1 cut(s)	559
PfoI	TCCNGGA	1 cut(s)	565
PkrI	GCNGC	9 cut(s)	86, 101, 218, 221, 224, 227, 636, 751, 754
PleI	GAGTC	2 cut(s)	31, 277
PpsI	GAGTC	2 cut(s)	31, 277
PshBI	ATTAAT	1 cut(s)	615
Psp6I	CCWGG	2 cut(s)	60, 639
PspFI	CCCAGC	1 cut(s)	46
PspGI	CCWGG	2 cut(s)	60, 639
PspN4I	GGNNCC	3 cut(s)	6, 479, 564
PspPI	GGNCC	2 cut(s)	75, 478
PstI	CTGCAG	1 cut(s)	636
PsuI	RGATCY	2 cut(s)	4, 724
PvuII	CAGCTG	2 cut(s)	392, 413
SaqAI	TTAA	2 cut(s)	129, 615
SatI	GCNGC	9 cut(s)	85, 100, 217, 220, 223, 226, 635, 750, 753
Sau3AI	GATC	4 cut(s)	4, 15, 528, 724
Sau96I	GGNCC	2 cut(s)	75, 478
SchI	GAGTC	2 cut(s)	32, 278
ScrFI	CCNGG	4 cut(s)	62, 93, 567, 641
SduI	GDGCHC	3 cut(s)	93, 120, 736
SexAI	ACCWGGT	1 cut(s)	639
SfaNI	GCATC	3 cut(s)	59, 560, 736
SfcI	CTRYAG	3 cut(s)	55, 486, 632
SfuI	TTCGAA	1 cut(s)	275
Sse9I	AATT	5 cut(s)	332, 361, 616, 676, 702
SspMI	CTAG	2 cut(s)	503, 516
StyD4I	CCNGG	4 cut(s)	60, 91, 565, 639
StyI	CCWWGG	1 cut(s)	779
TaaI	ACNGT	2 cut(s)	202, 500
TaiI	ACGT	1 cut(s)	854
TaqI	TCGA	5 cut(s)	197, 275, 645, 656, 840
TasI	AATT	5 cut(s)	332, 361, 616, 676, 702
TauI	GCSGC	6 cut(s)	87, 219, 222, 225, 228, 755
TfiI	GAWTC	2 cut(s)	194, 694
Tru1I	TTAA	2 cut(s)	129, 615
Tru9I	TTAA	2 cut(s)	129, 615
TscAI	CASTG	2 cut(s)	18, 636
TseFI	GTSAC	1 cut(s)	202
TseI	GCWGC	3 cut(s)	99, 634, 749
Tsp45I	GTSAC	1 cut(s)	202
TspDTI	ATGAA	5 cut(s)	344, 366, 557, 674, 707
TspRI	CASTG	2 cut(s)	18, 636
Van91I	CCANNNNNTGG	1 cut(s)	559
VneI	GTGCAC	1 cut(s)	732
VspI	ATTAAT	1 cut(s)	615
XmnI	GAANNNNTTC	1 cut(s)	711
XspI	CTAG	2 cut(s)	503, 516

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.