Rw4G009590

transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0002571
Physical Location & Seq
Reverse (-)
21628417 .. 21630085
1669 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G009590.1

Sequence Viewer

Length: 552 bp
ATGGTTTCTACTAGTAGTGCTAATGGCTTGAAAGATTCGAGTGGGAAACGGATGGAGTTAGAGGGATCTAGAAACGAAAACGGCAATTCTAAAGCTGAAGTTGAAACAAGTTCAGGGGCTGATGACAAGCCAGAGGAACAAAATTATATCCATGTGAGGGCGAGAAGGGGCCAAGCTACCTATAGCCACAGTCTCACCGAGAGAGAGAGAGAAAAGATCAGCCAGAGGATGAAAATTCTTCAGGATTTGGTTCCTGGATGTAATAAGGTTATTGGGAAAGCACTTGTTCTTGATGAGATTATTAATTACATCCAGTCACTGCAGCACCAAGTCGAGTTCCTTTCGTTGAACATTGAAGCAGTCAATTCAAGGGTGAACATGAATCCCACCATTGAAGCGTTTCCTTCAAAAGATGTGTTTTTTCCTGGTGCACAGCAATTTGATGCAGCTGTGTTGTTATTTGGACCACACACACCAAGGGAGTATGTGCAGGGCTCGCAAGCCGAATGGCTGCATATGCAGGTTGGTGGTTGTCTTGATTTTGTTTCATAG

Protein Analysis

183

Amino Acids

20.37

Weight (kDa)

5.56

Isoelectric Point (pI)

43.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
bHLH_SAC51 PF23173 69 - 110 3.3e-07 Transcription factor SAC51 bHLH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 511
AclWI GGATC 1 cut(s) 73
AcsI RAATTY 1 cut(s) 234
AcuI CTGAAG 2 cut(s) 117, 224
AfiI CCNNNNNNNGG 1 cut(s) 157
AgsI TTSAA 7 cut(s) 31, 104, 349, 356, 369, 395, 408
AhlI ACTAGT 1 cut(s) 11
AjnI CCWGG 2 cut(s) 253, 424
AluBI AGCT 3 cut(s) 95, 176, 449
AluI AGCT 3 cut(s) 95, 176, 449
Alw21I GWGCWC 1 cut(s) 433
Alw26I GTCTC 1 cut(s) 197
Alw44I GTGCAC 1 cut(s) 429
AlwI GGATC 1 cut(s) 73
AlwNI CAGNNNCTG 2 cut(s) 119, 319
AoxI GGCC 1 cut(s) 169
ApaLI GTGCAC 1 cut(s) 429
ApeKI GCWGC 3 cut(s) 322, 446, 511
ApoI RAATTY 1 cut(s) 234
AseI ATTAAT 1 cut(s) 303
Asp700I GAANNNNTTC 1 cut(s) 399
AspS9I GGNCC 2 cut(s) 169, 464
AsuHPI GGTGA 2 cut(s) 187, 385
AvaII GGWCC 1 cut(s) 464
BaeGI GKGCMC 1 cut(s) 433
BanII GRGCYC 1 cut(s) 497
Bbv12I GWGCWC 1 cut(s) 433
BbvI GCAGC 3 cut(s) 334, 458, 498
BccI CCATC 1 cut(s) 46
BceAI ACGGC 1 cut(s) 97
BciT130I CCWGG 2 cut(s) 255, 426
BcoDI GTCTC 1 cut(s) 197
BcuI ACTAGT 1 cut(s) 11
BfaI CTAG 2 cut(s) 12, 69
BfmI CTRYAG 2 cut(s) 181, 320
BfuAI ACCTGC 1 cut(s) 511
BisI GCNGC 3 cut(s) 323, 447, 512
BlsI GCNGC 3 cut(s) 324, 448, 513
Bme1390I CCNGG 2 cut(s) 255, 426
Bme18I GGWCC 1 cut(s) 464
BmgT120I GGNCC 2 cut(s) 169, 464
BmiI GGNNCC 2 cut(s) 170, 252
BmrFI CCNGG 2 cut(s) 255, 426
BmsI GCATC 1 cut(s) 433
BsaJI CCNNGG 1 cut(s) 476
Bsc4I CCNNNNNNNGG 1 cut(s) 157
Bse1I ACTGG 1 cut(s) 313
BseBI CCWGG 2 cut(s) 255, 426
BseDI CCNNGG 1 cut(s) 476
BseGI GGATG 4 cut(s) 57, 234, 263, 309
BseLI CCNNNNNNNGG 1 cut(s) 157
BseNI ACTGG 1 cut(s) 313
BseSI GKGCMC 1 cut(s) 433
BseXI GCAGC 3 cut(s) 334, 458, 498
BsgI GTGCAG 1 cut(s) 509
BshFI GGCC 1 cut(s) 171
BsiHKAI GWGCWC 1 cut(s) 433
BslI CCNNNNNNNGG 1 cut(s) 157
BsmAI GTCTC 1 cut(s) 197
BsnI GGCC 1 cut(s) 171
Bsp1286I GDGCHC 2 cut(s) 433, 497
Bsp143I GATC 2 cut(s) 65, 216
BspANI GGCC 1 cut(s) 171
BspLI GGNNCC 2 cut(s) 170, 252
BspMAI CTGCAG 1 cut(s) 324
BspMI ACCTGC 1 cut(s) 511
BspPI GGATC 1 cut(s) 73
BsrI ACTGG 1 cut(s) 313
BssECI CCNNGG 1 cut(s) 476
BssMI GATC 2 cut(s) 65, 216
BssT1I CCWWGG 1 cut(s) 476
Bst2UI CCWGG 2 cut(s) 255, 426
Bst4CI ACNGT 1 cut(s) 191
BstC8I GCNNGC 2 cut(s) 497, 501
BstF5I GGATG 4 cut(s) 57, 234, 263, 309
BstKTI GATC 2 cut(s) 68, 219
BstMAI GTCTC 1 cut(s) 197
BstMBI GATC 2 cut(s) 65, 216
BstMWI GCNNNNNNNGC 2 cut(s) 496, 517
BstNI CCWGG 2 cut(s) 255, 426
BstSCI CCNGG 2 cut(s) 253, 424
BstSFI CTRYAG 2 cut(s) 181, 320
BstSLI GKGCMC 1 cut(s) 433
BstV1I GCAGC 3 cut(s) 334, 458, 498
BstX2I RGATCY 1 cut(s) 65
BstYI RGATCY 1 cut(s) 65
BsuRI GGCC 1 cut(s) 171
BtsCI GGATG 4 cut(s) 57, 234, 263, 309
BtsI GCAGTG 1 cut(s) 317
BtsIMutI CAGTG 1 cut(s) 317
BveI ACCTGC 1 cut(s) 511
Cac8I GCNNGC 2 cut(s) 497, 501
CaiI CAGNNNCTG 2 cut(s) 119, 319
Cfr13I GGNCC 2 cut(s) 169, 464
CviAII CATG 2 cut(s) 152, 379
DpnI GATC 2 cut(s) 67, 218
DpnII GATC 2 cut(s) 65, 216
Eco130I CCWWGG 1 cut(s) 476
Eco24I GRGCYC 1 cut(s) 497
Eco47I GGWCC 1 cut(s) 464
Eco57I CTGAAG 2 cut(s) 117, 224
EcoRII CCWGG 2 cut(s) 253, 424
EcoT14I CCWWGG 1 cut(s) 476
EcoT38I GRGCYC 1 cut(s) 497
ErhI CCWWGG 1 cut(s) 476
FaeI CATG 2 cut(s) 155, 382
FaiI YATR 8 cut(s) 147, 153, 183, 380, 486, 516, 518, 550
FatI CATG 2 cut(s) 151, 378
FauNDI CATATG 1 cut(s) 516
Fnu4HI GCNGC 3 cut(s) 323, 447, 512
FokI GGATG 4 cut(s) 64, 241, 270, 296
FriOI GRGCYC 1 cut(s) 497
Fsp4HI GCNGC 3 cut(s) 323, 447, 512
FspBI CTAG 2 cut(s) 12, 69
GluI GCNGC 3 cut(s) 323, 447, 512
HaeIII GGCC 1 cut(s) 171
Hin1II CATG 2 cut(s) 155, 382
HinfI GANTC 2 cut(s) 35, 382
HphI GGTGA 2 cut(s) 187, 385
Hpy166II GTNNAC 2 cut(s) 376, 431
Hpy188III TCNNGA 4 cut(s) 69, 242, 290, 536
Hpy8I GTNNAC 2 cut(s) 376, 431
HpyAV CCTTC 2 cut(s) 159, 414
HpyCH4III ACNGT 1 cut(s) 191
HpyCH4V TGCA 6 cut(s) 322, 431, 446, 490, 514, 520
HpyF10VI GCNNNNNNNGC 2 cut(s) 496, 517
Hsp92II CATG 2 cut(s) 155, 382
Kzo9I GATC 2 cut(s) 65, 216
Lsp1109I GCAGC 3 cut(s) 334, 458, 498
LweI GCATC 1 cut(s) 433
MaeI CTAG 2 cut(s) 12, 69
MaeIII GTNAC 1 cut(s) 315
MalI GATC 2 cut(s) 67, 218
MboI GATC 2 cut(s) 65, 216
MboII GAAGA 1 cut(s) 230
MflI RGATCY 1 cut(s) 65
MhlI GDGCHC 2 cut(s) 433, 497
MluCI AATT 6 cut(s) 85, 142, 234, 304, 364, 437
MnlI CCTC 4 cut(s) 55, 127, 150, 219
MroXI GAANNNNTTC 1 cut(s) 399
MseI TTAA 1 cut(s) 303
MspA1I CMGCKG 1 cut(s) 449
MspR9I CCNGG 2 cut(s) 255, 426
MvaI CCWGG 2 cut(s) 255, 426
MwoI GCNNNNNNNGC 2 cut(s) 496, 517
NdeI CATATG 1 cut(s) 516
NdeII GATC 2 cut(s) 65, 216
NlaIII CATG 2 cut(s) 155, 382
NlaIV GGNNCC 2 cut(s) 170, 252
NmuCI GTSAC 1 cut(s) 315
PdmI GAANNNNTTC 1 cut(s) 399
PfeI GAWTC 2 cut(s) 35, 382
PfoI TCCNGGA 1 cut(s) 253
PkrI GCNGC 3 cut(s) 324, 448, 513
PshBI ATTAAT 1 cut(s) 303
Psp6I CCWGG 2 cut(s) 253, 424
PspGI CCWGG 2 cut(s) 253, 424
PspN4I GGNNCC 2 cut(s) 170, 252
PspPI GGNCC 2 cut(s) 169, 464
PstI CTGCAG 1 cut(s) 324
PstNI CAGNNNCTG 2 cut(s) 119, 319
PsuI RGATCY 1 cut(s) 65
PvuII CAGCTG 1 cut(s) 449
SaqAI TTAA 1 cut(s) 303
SatI GCNGC 3 cut(s) 323, 447, 512
Sau3AI GATC 2 cut(s) 65, 216
Sau96I GGNCC 2 cut(s) 169, 464
ScrFI CCNGG 2 cut(s) 255, 426
SduI GDGCHC 2 cut(s) 433, 497
SetI ASST 6 cut(s) 97, 178, 182, 270, 451, 525
SfaNI GCATC 1 cut(s) 433
SfcI CTRYAG 2 cut(s) 181, 320
SinI GGWCC 1 cut(s) 464
SpeI ACTAGT 1 cut(s) 11
Sse9I AATT 6 cut(s) 85, 142, 234, 304, 364, 437
SspMI CTAG 2 cut(s) 12, 69
StyD4I CCNGG 2 cut(s) 253, 424
StyI CCWWGG 1 cut(s) 476
TaaI ACNGT 1 cut(s) 191
TaqI TCGA 2 cut(s) 38, 333
TasI AATT 6 cut(s) 85, 142, 234, 304, 364, 437
TfiI GAWTC 2 cut(s) 35, 382
Tru1I TTAA 1 cut(s) 303
Tru9I TTAA 1 cut(s) 303
TscAI CASTG 1 cut(s) 324
TseFI GTSAC 1 cut(s) 315
TseI GCWGC 3 cut(s) 322, 446, 511
Tsp45I GTSAC 1 cut(s) 315
TspDTI ATGAA 3 cut(s) 245, 395, 537
TspGWI ACGGA 1 cut(s) 64
TspRI CASTG 1 cut(s) 324
VneI GTGCAC 1 cut(s) 429
VpaK11BI GGWCC 1 cut(s) 464
VspI ATTAAT 1 cut(s) 303
XapI RAATTY 1 cut(s) 234
XbaI TCTAGA 1 cut(s) 68
XmnI GAANNNNTTC 1 cut(s) 399
XspI CTAG 2 cut(s) 12, 69
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.