MD15G1355200.v1.1

Catalyzes the prenylation of para-hydroxybenzoate (PHB) with an all-trans polyprenyl group. Mediates the second step in the final reaction sequence of coenzyme Q (CoQ) biosynthesis, which is the condensation of the polyisoprenoid side chain with PHB, generating the first membrane-bound Q intermediate

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0001284
Physical Location & Seq
Forward (+)
42778773 .. 42782319
3547 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1355200.v1.1.491

Sequence Viewer

Length: 1188 bp
ATGGCTTCGTTTTGGCTGGCCTCTCGCTCGTCACGTAGGTTTCTGAAACCTTCAGTGTCCCTCTCCCGCACCCTATTCGCAGTCTCTTCTTATCACCCAACTACAGCCACCGCCATTCCTAACCCTAACCCTAACCCTTATCAGAACGCAAGCCCGTTTGGCCCATTCAATCAAAGGCACCGTTGGAGTCCAAGCTCCGTTGAATTTGATCTCAGACTCTTAGCGCACCTCTCCAGCTTGCCTTCGTCAACTGGCGAGAAGAACGACGGAAATCAGGGCGGCCATGGCACGAAGGCTGAGGCTTCTTGGATCGACTTGTATTTGCCGAGGCAAGCTCGGCCTTATGCTAAGCTCGCTCGCCTTGACCGGCCCATTGGCACCTGGTTGCTCGCTTGGCCTTGTATGTGGTCAATAACATTGGCAGCAAGCCCAGGGCACCTTCCTGATTTTAAGATGATGACACTATTTGGATGTGGCGCTCTGCTTTTGAGGGGCGCTGGATGCACCGTAAATGATCTCCTTGACCGGGATATTGATACCATGGTCGCACGCACAAAGTTGCGGCCAGTTGCAAGTGGTGTTTTGACACCCTTTCAGGGGCTTTGCTTTCTTGGATTTCAATTGCTTTTGGGTCTTGGGATTCTCCTCCAACTGAATAATTATAGCCGTGTTTTAGGGGCTTCATCTTTGTTGCTAGTTTTCTCCTATCCTCTCATGAAGAGGTTCACATTCTGGCCTCAAGCCTATTTAGGGTTAACCTTCAATTGGGGAGCTTTGTTAGGATGGGCTGCTGTTAAAGGAAGCATTGATCCAGCTGTAGTGCTCCCATTGTACTTTTCTGGAGTATGCTGGACACTTGTGTATGATACGATATATGCACATCAGGACAAAGAAGATGATTTGAAAGTAGGTGTTAAGTCTACGGCTTTGAGATTTGACGATTCAACTAAGGAGTGGATTACTGGCTTTGGAATTTTGTGCATGAGTAGTCTTGCCCTTAGTGGATATAATGCTGAAATTGGTTGGCCATATTATGCATTTTTGTCGGCTGCTTCTGGACAATTAGCTTGGCAGATATGGACAGCTGACCTTTCATCCCGGGCTGATTGCAATAGAAAATTTGTGTCGAACAAATGGTTTGGTGCTATTATTTTCAGTGGAATCCTGTTTGGAAGACTTTCATCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00130 ko01100 ko01110 map00130 map01100 map01110
Pfam Domains
Protein Families
UbiA

Protein Analysis

396

Amino Acids

43.65

Weight (kDa)

9.36

Isoelectric Point (pI)

39.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UbiA PF01040 131 - 376 9.8e-59 UbiA prenyltransferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 177, 377, 435
AccI GTMKAC 1 cut(s) 920
AciI CCGC 4 cut(s) 67, 111, 279, 562
AclWI GGATC 2 cut(s) 317, 803
AcoI YGGCCR 3 cut(s) 280, 563, 1025
AcsI RAATTY 3 cut(s) 203, 972, 1118
AcuI CTGAAG 1 cut(s) 36
AfaI GTAC 1 cut(s) 833
AfiI CCNNNNNNNGG 5 cut(s) 526, 596, 597, 750, 765
AgsI TTSAA 6 cut(s) 169, 203, 620, 763, 904, 945
AjnI CCWGG 2 cut(s) 380, 430
AluBI AGCT 8 cut(s) 195, 237, 335, 352, 773, 815, 1067, 1085
AluI AGCT 8 cut(s) 195, 237, 335, 352, 773, 815, 1067, 1085
Alw21I GWGCWC 1 cut(s) 825
Alw26I GTCTC 1 cut(s) 88
AlwI GGATC 2 cut(s) 317, 803
Ama87I CYCGRG 1 cut(s) 1098
AoxI GGCC 9 cut(s) 18, 160, 280, 338, 368, 395, 563, 734, 1025
ApeKI GCWGC 3 cut(s) 422, 788, 1049
ApoI RAATTY 3 cut(s) 203, 972, 1118
Asp700I GAANNNNTTC 2 cut(s) 722, 1177
AspLEI GCGC 3 cut(s) 226, 479, 497
AspS9I GGNCC 2 cut(s) 161, 369
AsuC2I CCSGG 3 cut(s) 527, 1099, 1100
AsuHPI GGTGA 1 cut(s) 86
AvaI CYCGRG 1 cut(s) 1098
BaeGI GKGCMC 1 cut(s) 438
BaeI ACNNNNGTAYC 2 cut(s) 528, 561
BalI TGGCCA 1 cut(s) 1027
BanI GGYRCC 3 cut(s) 177, 377, 435
BbsI GAAGAC 1 cut(s) 1180
Bbv12I GWGCWC 1 cut(s) 825
BbvCI CCTCAGC 1 cut(s) 297
BbvI GCAGC 3 cut(s) 434, 775, 1036
BccI CCATC 1 cut(s) 777
BceAI ACGGC 2 cut(s) 651, 939
BcgI CGANNNNNNTGC 4 cut(s) 58, 92, 1026, 1060
BciT130I CCWGG 2 cut(s) 382, 432
BcnI CCSGG 3 cut(s) 527, 1099, 1100
BcoDI GTCTC 1 cut(s) 88
BfaI CTAG 1 cut(s) 695
BfmI CTRYAG 2 cut(s) 102, 816
BfoI RGCGCY 2 cut(s) 480, 498
BglI GCCNNNNNGGC 1 cut(s) 159
BisI GCNGC 5 cut(s) 280, 423, 563, 789, 1050
BlpI GCTNAGC 1 cut(s) 348
BlsI GCNGC 5 cut(s) 281, 424, 564, 790, 1051
Bme1390I CCNGG 5 cut(s) 382, 432, 527, 1099, 1100
BmeT110I CYCGRG 1 cut(s) 1098
BmgT120I GGNCC 2 cut(s) 161, 369
BmiI GGNNCC 3 cut(s) 179, 379, 437
BmrFI CCNGG 5 cut(s) 382, 432, 527, 1099, 1100
BmsI GCATC 1 cut(s) 491
BpiI GAAGAC 1 cut(s) 1180
BplI GAGNNNNNCTC 2 cut(s) 319, 351
BpmI CTGGAG 2 cut(s) 217, 861
Bpu10I CCTNAGC 1 cut(s) 297
Bpu1102I GCTNAGC 1 cut(s) 348
BpuEI CTTGAG 1 cut(s) 723
BpuMI CCSGG 3 cut(s) 527, 1099, 1100
BsaAI YACGTR 1 cut(s) 35
BsaJI CCNNGG 6 cut(s) 283, 326, 430, 431, 540, 1098
Bsc4I CCNNNNNNNGG 5 cut(s) 526, 596, 597, 750, 765
Bse118I RCCGGY 1 cut(s) 366
Bse1I ACTGG 3 cut(s) 256, 566, 967
BseBI CCWGG 2 cut(s) 382, 432
BseDI CCNNGG 6 cut(s) 283, 326, 430, 431, 540, 1098
BseGI GGATG 4 cut(s) 476, 506, 788, 1094
BseLI CCNNNNNNNGG 5 cut(s) 526, 596, 597, 750, 765
BseMII CTCAG 2 cut(s) 226, 288
BseNI ACTGG 3 cut(s) 256, 566, 967
BseRI GAGGAG 1 cut(s) 635
BseSI GKGCMC 1 cut(s) 438
BseXI GCAGC 3 cut(s) 434, 775, 1036
BshFI GGCC 9 cut(s) 20, 162, 282, 340, 370, 397, 565, 736, 1027
BshNI GGYRCC 3 cut(s) 177, 377, 435
BsiHKAI GWGCWC 1 cut(s) 825
BsiHKCI CYCGRG 1 cut(s) 1098
BsiSI CCGG 3 cut(s) 367, 526, 1099
BslFI GGGAC 1 cut(s) 43
BslI CCNNNNNNNGG 5 cut(s) 526, 596, 597, 750, 765
BsmAI GTCTC 1 cut(s) 88
BsmFI GGGAC 1 cut(s) 43
BsnI GGCC 9 cut(s) 20, 162, 282, 340, 370, 397, 565, 736, 1027
BsoBI CYCGRG 1 cut(s) 1098
Bsp1286I GDGCHC 2 cut(s) 438, 825
Bsp143I GATC 4 cut(s) 208, 309, 514, 808
Bsp1720I GCTNAGC 1 cut(s) 348
Bsp19I CCATGG 2 cut(s) 283, 540
BspACI CCGC 4 cut(s) 67, 111, 279, 562
BspANI GGCC 9 cut(s) 20, 162, 282, 340, 370, 397, 565, 736, 1027
BspCNI CTCAG 2 cut(s) 225, 289
BspHI TCATGA 1 cut(s) 714
BspLI GGNNCC 3 cut(s) 179, 379, 437
BspPI GGATC 2 cut(s) 317, 803
BspT107I GGYRCC 3 cut(s) 177, 377, 435
BsrFI RCCGGY 1 cut(s) 366
BsrI ACTGG 3 cut(s) 256, 566, 967
BssAI RCCGGY 1 cut(s) 366
BssECI CCNNGG 6 cut(s) 283, 326, 430, 431, 540, 1098
BssMI GATC 4 cut(s) 208, 309, 514, 808
BssT1I CCWWGG 2 cut(s) 283, 540
Bst2UI CCWGG 2 cut(s) 382, 432
Bst4CI ACNGT 2 cut(s) 182, 508
Bst6I CTCTTC 2 cut(s) 91, 713
BstBAI YACGTR 1 cut(s) 35
BstC8I GCNNGC 9 cut(s) 18, 151, 239, 333, 354, 358, 390, 427, 550
BstDEI CTNAG 7 cut(s) 212, 220, 297, 348, 948, 998, 1185
BstDSI CCRYGG 2 cut(s) 283, 540
BstF5I GGATG 4 cut(s) 476, 506, 788, 1094
BstH2I RGCGCY 2 cut(s) 480, 498
BstHHI GCGC 3 cut(s) 226, 479, 497
BstKTI GATC 4 cut(s) 211, 312, 517, 811
BstMAI GTCTC 1 cut(s) 88
BstMBI GATC 4 cut(s) 208, 309, 514, 808
BstMWI GCNNNNNNNGC 6 cut(s) 159, 285, 337, 353, 394, 501
BstNI CCWGG 2 cut(s) 382, 432
BstSCI CCNGG 5 cut(s) 380, 430, 525, 1097, 1098
BstSFI CTRYAG 2 cut(s) 102, 816
BstSLI GKGCMC 1 cut(s) 438
BstV1I GCAGC 3 cut(s) 434, 775, 1036
BstV2I GAAGAC 1 cut(s) 1180
BsuRI GGCC 9 cut(s) 20, 162, 282, 340, 370, 397, 565, 736, 1027
BtgI CCRYGG 2 cut(s) 283, 540
BtsCI GGATG 4 cut(s) 476, 506, 788, 1094
BtsIMutI CAGTG 2 cut(s) 60, 1162
Cac8I GCNNGC 9 cut(s) 18, 151, 239, 333, 354, 358, 390, 427, 550
CciI TCATGA 1 cut(s) 714
CfoI GCGC 3 cut(s) 226, 479, 497
Cfr10I RCCGGY 1 cut(s) 366
Cfr13I GGNCC 2 cut(s) 161, 369
Cfr9I CCCGGG 1 cut(s) 1098
CsiI ACCWGGT 1 cut(s) 380
Csp6I GTAC 1 cut(s) 832
CviAII CATG 4 cut(s) 284, 541, 715, 982
CviQI GTAC 1 cut(s) 832
DdeI CTNAG 7 cut(s) 212, 220, 297, 348, 948, 998, 1185
DpnI GATC 4 cut(s) 210, 311, 516, 810
DpnII GATC 4 cut(s) 208, 309, 514, 808
EaeI YGGCCR 3 cut(s) 280, 563, 1025
Eam1104I CTCTTC 2 cut(s) 91, 713
EarI CTCTTC 2 cut(s) 91, 713
Eco130I CCWWGG 2 cut(s) 283, 540
Eco57I CTGAAG 1 cut(s) 36
Eco88I CYCGRG 1 cut(s) 1098
EcoRII CCWGG 2 cut(s) 380, 430
EcoT14I CCWWGG 2 cut(s) 283, 540
EcoT22I ATGCAT 1 cut(s) 1039
ErhI CCWWGG 2 cut(s) 283, 540
FaeI CATG 4 cut(s) 287, 544, 718, 985
FaqI GGGAC 1 cut(s) 43
FatI CATG 4 cut(s) 283, 540, 714, 981
FauI CCCGC 1 cut(s) 74
FblI GTMKAC 1 cut(s) 920
Fnu4HI GCNGC 5 cut(s) 280, 423, 563, 789, 1050
FokI GGATG 4 cut(s) 483, 513, 795, 1081
Fsp4HI GCNGC 5 cut(s) 280, 423, 563, 789, 1050
FspBI CTAG 1 cut(s) 695
GlaI GCGC 3 cut(s) 225, 478, 496
GluI GCNGC 5 cut(s) 280, 423, 563, 789, 1050
GsuI CTGGAG 2 cut(s) 217, 861
HaeII RGCGCY 2 cut(s) 480, 498
HaeIII GGCC 9 cut(s) 20, 162, 282, 340, 370, 397, 565, 736, 1027
HapII CCGG 3 cut(s) 367, 526, 1099
HhaI GCGC 3 cut(s) 226, 479, 497
Hin1II CATG 4 cut(s) 287, 544, 718, 985
Hin6I GCGC 3 cut(s) 224, 477, 495
HinP1I GCGC 3 cut(s) 224, 477, 495
HincII GTYRAC 2 cut(s) 249, 756
HindII GTYRAC 2 cut(s) 249, 756
HinfI GANTC 5 cut(s) 187, 216, 640, 941, 1161
HpaI GTTAAC 1 cut(s) 756
HpaII CCGG 3 cut(s) 367, 526, 1099
HphI GGTGA 1 cut(s) 86
Hpy166II GTNNAC 4 cut(s) 249, 726, 756, 921
Hpy188I TCNGA 3 cut(s) 45, 144, 215
Hpy188III TCNNGA 5 cut(s) 443, 715, 840, 884, 1056
Hpy8I GTNNAC 4 cut(s) 249, 726, 756, 921
Hpy99I CGWCG 1 cut(s) 269
HpyAV CCTTC 5 cut(s) 60, 252, 286, 449, 769
HpyCH4III ACNGT 2 cut(s) 182, 508
HpyCH4IV ACGT 1 cut(s) 34
HpyCH4V TGCA 6 cut(s) 504, 572, 878, 981, 1037, 1110
HpyF10VI GCNNNNNNNGC 6 cut(s) 159, 285, 337, 353, 394, 501
HpyF3I CTNAG 7 cut(s) 212, 220, 297, 348, 948, 998, 1185
HpySE526I ACGT 1 cut(s) 34
Hsp92II CATG 4 cut(s) 287, 544, 718, 985
HspAI GCGC 3 cut(s) 224, 477, 495
KspAI GTTAAC 1 cut(s) 756
Kzo9I GATC 4 cut(s) 208, 309, 514, 808
LmnI GCTCC 3 cut(s) 200, 770, 828
Lsp1109I GCAGC 3 cut(s) 434, 775, 1036
LweI GCATC 1 cut(s) 491
MabI ACCWGGT 1 cut(s) 380
MaeI CTAG 1 cut(s) 695
MaeII ACGT 1 cut(s) 34
MaeIII GTNAC 1 cut(s) 30
MalI GATC 4 cut(s) 210, 311, 516, 810
MboI GATC 4 cut(s) 208, 309, 514, 808
MboII GAAGA 5 cut(s) 78, 271, 730, 905, 1185
MfeI CAATTG 2 cut(s) 620, 763
MhlI GDGCHC 2 cut(s) 438, 825
MlsI TGGCCA 1 cut(s) 1027
MluCI AATT 8 cut(s) 203, 620, 658, 763, 972, 1017, 1061, 1118
MluNI TGGCCA 1 cut(s) 1027
MlyI GAGTC 2 cut(s) 196, 210
MmeI TCCRAC 2 cut(s) 164, 673
Mox20I TGGCCA 1 cut(s) 1027
Mph1103I ATGCAT 1 cut(s) 1039
MroXI GAANNNNTTC 2 cut(s) 722, 1177
MscI TGGCCA 1 cut(s) 1027
MseI TTAA 4 cut(s) 450, 755, 795, 915
Msp20I TGGCCA 1 cut(s) 1027
MspA1I CMGCKG 2 cut(s) 815, 1085
MspI CCGG 3 cut(s) 367, 526, 1099
MspR9I CCNGG 5 cut(s) 382, 432, 527, 1099, 1100
MunI CAATTG 2 cut(s) 620, 763
MvaI CCWGG 2 cut(s) 382, 432
MwoI GCNNNNNNNGC 6 cut(s) 159, 285, 337, 353, 394, 501
NciI CCSGG 3 cut(s) 527, 1099, 1100
NcoI CCATGG 2 cut(s) 283, 540
NdeII GATC 4 cut(s) 208, 309, 514, 808
NlaIII CATG 4 cut(s) 287, 544, 718, 985
NlaIV GGNNCC 3 cut(s) 179, 379, 437
NmeAIII GCCGAG 2 cut(s) 316, 351
NmuCI GTSAC 1 cut(s) 30
NsiI ATGCAT 1 cut(s) 1039
PagI TCATGA 1 cut(s) 714
PasI CCCWGGG 1 cut(s) 431
PcsI WCGNNNNNNNCGW 1 cut(s) 31
PdmI GAANNNNTTC 2 cut(s) 722, 1177
PfeI GAWTC 3 cut(s) 640, 941, 1161
PkrI GCNGC 5 cut(s) 281, 424, 564, 790, 1051
PleI GAGTC 2 cut(s) 195, 210
PpsI GAGTC 2 cut(s) 195, 210
Ppu21I YACGTR 1 cut(s) 35
Psp6I CCWGG 2 cut(s) 380, 430
PspGI CCWGG 2 cut(s) 380, 430
PspN4I GGNNCC 3 cut(s) 179, 379, 437
PspPI GGNCC 2 cut(s) 161, 369
PvuII CAGCTG 2 cut(s) 815, 1085
RsaI GTAC 1 cut(s) 833
RsaNI GTAC 1 cut(s) 832
SaqAI TTAA 4 cut(s) 450, 755, 795, 915
SatI GCNGC 5 cut(s) 280, 423, 563, 789, 1050
Sau3AI GATC 4 cut(s) 208, 309, 514, 808
Sau96I GGNCC 2 cut(s) 161, 369
SchI GAGTC 2 cut(s) 196, 210
ScrFI CCNGG 5 cut(s) 382, 432, 527, 1099, 1100
SduI GDGCHC 2 cut(s) 438, 825
SexAI ACCWGGT 1 cut(s) 380
SfaNI GCATC 1 cut(s) 491
SfcI CTRYAG 2 cut(s) 102, 816
SmaI CCCGGG 1 cut(s) 1100
SmlI CTYRAG 1 cut(s) 738
SmoI CTYRAG 1 cut(s) 738
Sse9I AATT 8 cut(s) 203, 620, 658, 763, 972, 1017, 1061, 1118
SsiI CCGC 4 cut(s) 67, 111, 279, 562
SspMI CTAG 1 cut(s) 695
StyD4I CCNGG 5 cut(s) 380, 430, 525, 1097, 1098
StyI CCWWGG 2 cut(s) 283, 540
TaaI ACNGT 2 cut(s) 182, 508
TaiI ACGT 1 cut(s) 37
TaqI TCGA 2 cut(s) 312, 1127
TasI AATT 8 cut(s) 203, 620, 658, 763, 972, 1017, 1061, 1118
TatI WGTACW 1 cut(s) 831
TauI GCSGC 2 cut(s) 282, 565
TfiI GAWTC 3 cut(s) 640, 941, 1161
Tru1I TTAA 4 cut(s) 450, 755, 795, 915
Tru9I TTAA 4 cut(s) 450, 755, 795, 915
TscAI CASTG 2 cut(s) 60, 1162
TseFI GTSAC 1 cut(s) 30
TseI GCWGC 3 cut(s) 422, 788, 1049
Tsp45I GTSAC 1 cut(s) 30
TspDTI ATGAA 4 cut(s) 672, 731, 1083, 1170
TspGWI ACGGA 2 cut(s) 187, 282
TspMI CCCGGG 1 cut(s) 1098
TspRI CASTG 2 cut(s) 60, 1162
XapI RAATTY 3 cut(s) 203, 972, 1118
XmaI CCCGGG 1 cut(s) 1098
XmiI GTMKAC 1 cut(s) 920
XmnI GAANNNNTTC 2 cut(s) 722, 1177
XspI CTAG 1 cut(s) 695
Zsp2I ATGCAT 1 cut(s) 1039
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.