Rw5G027920

Catalyzes the prenylation of para-hydroxybenzoate (PHB) with an all-trans polyprenyl group. Mediates the second step in the final reaction sequence of coenzyme Q (CoQ) biosynthesis, which is the condensation of the polyisoprenoid side chain with PHB, generating the first membrane-bound Q intermediate

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0001284
Physical Location & Seq
Reverse (-)
41642738 .. 41645273
2536 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G027920.1

Sequence Viewer

Length: 1149 bp
ATGACGCCGTTTCGGCTCTTGTCTCGGGCATCGCGTAGGCTTCTGAAGCCTCCTTCACTCTCTCGCTCCCATCTCTCTGCTGCAGTCTCTACCTATCACCCAACCACCAAACCCAGCCCTAAAGAAACGCCTTTCATCCCTTTTAATCTCAGGCACCGTTGGATTCCCGGTTATCTTGATTCTGATCTCAGGCTCGTAGCGCACATATCCACTTCATCGAACAAGGACGACAACCGAGTCAGCGATGACCCGAAAGCCCAAGCTTCTTGGATCGACGCTTATTTGCCCAGGCAGGCCCGCCCTTATGCTAAGCTCGCTCGCCTCGACAAGCCCATTGGCACCTGGTTGCTTGCTTGGCCTTGCATGTGGTCAATAACATTGGCAGCAACCCCAGGACACCTTCCTGATATTAAGATGTTGACACTATTTGGATGTGGGGCTTTGCTTTTAAGGGGCGCTGGATGTACCGTAAATGATCTCATTGATCGGGATATTGACACCATGGTGGAACGTACAAAGTTGCGGCCAGTTGCGAGTGGTCTTTTGACACCTTTTAAAGGGTTGTGTTTTCTTGGATTTCAATTGCTTTTGGGTCTTGGAATTCTCCTCCAACTGAATAATTATAGCCGTGTTTTGGGGGCTTCGTCTTTGTTGCTAGTTTTCTCCTATCCCCTCATGAAAAGGTTGACATTCTGGCCTCAAGCATACCTAGGTTTGACCTTTAACTGGGGAGCTTTGTTAGGATGGGCTGCAATTAAAGGAAGCATTGATCCAGCTATAATAGTTCCGTTGTACATTTCTGGAGTATTTTGGACACTTGTGTATGATACGATATATGCGCATCAGGATAAAGAAGATGATCTGAAGGTTGGTGTCAAATCTACCGCTTTGAGGTTTGGTGATTCAACAAAGGAGTGGATTACTGGGTTTGGAATCCTGTGCATCAGTAGTCTTGCCCTCAGTGGATATAATGCCGAAATTGGCTGGCCATATTATGCACTTTTGGCAGTCGCATCTGGACAATTAGCGTGGCAGATATGGACAGTTGACCTTTCATCCAGGCTTGATTGCAATAGAAAGTTTGTGTCAAACAAGTGGTTTGGTGCTATTATTTGCAGTGGAATCTTATTGGGAAGACTTTCATCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00130 ko01100 ko01110 map00130 map01100 map01110
Pfam Domains
Protein Families
UbiA

Protein Analysis

382

Amino Acids

42.44

Weight (kDa)

9.51

Isoelectric Point (pI)

34.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UbiA PF01040 118 - 363 2.1e-60 UbiA prenyltransferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 236
Acc16I TGCGCA 1 cut(s) 840
AccB1I GGYRCC 2 cut(s) 153, 338
AccII CGCG 1 cut(s) 34
AciI CCGC 3 cut(s) 298, 523, 885
AclWI GGATC 2 cut(s) 278, 764
AcoI YGGCCR 2 cut(s) 524, 986
AcsI RAATTY 1 cut(s) 600
AcuI CTGAAG 2 cut(s) 65, 884
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 3 cut(s) 466, 514, 794
AfiI CCNNNNNNNGG 4 cut(s) 557, 634, 726, 891
AgsI TTSAA 2 cut(s) 581, 906
AjnI CCWGG 4 cut(s) 287, 341, 391, 1058
AleI CACNNNNGTG 1 cut(s) 503
AluBI AGCT 4 cut(s) 263, 313, 734, 776
AluI AGCT 4 cut(s) 263, 313, 734, 776
Alw26I GTCTC 2 cut(s) 27, 91
AlwI GGATC 2 cut(s) 278, 764
Ama87I CYCGRG 1 cut(s) 24
AoxI GGCC 5 cut(s) 294, 356, 524, 695, 986
ApeKI GCWGC 3 cut(s) 80, 383, 749
ApoI RAATTY 1 cut(s) 600
Asp700I GAANNNNTTC 1 cut(s) 1138
AspA2I CCTAGG 1 cut(s) 709
AspLEI GCGC 3 cut(s) 202, 458, 841
AspS9I GGNCC 1 cut(s) 295
AsuC2I CCSGG 1 cut(s) 168
AsuHPI GGTGA 2 cut(s) 89, 911
AvaI CYCGRG 1 cut(s) 24
AvrII CCTAGG 1 cut(s) 709
BalI TGGCCA 1 cut(s) 988
BanI GGYRCC 2 cut(s) 153, 338
BbsI GAAGAC 1 cut(s) 1141
BbvI GCAGC 3 cut(s) 67, 395, 736
BccI CCATC 2 cut(s) 78, 738
BceAI ACGGC 1 cut(s) 612
BciT130I CCWGG 4 cut(s) 289, 343, 393, 1060
BcnI CCSGG 1 cut(s) 168
BcoDI GTCTC 2 cut(s) 27, 91
BfaI CTAG 2 cut(s) 656, 710
BfmI CTRYAG 1 cut(s) 81
BfoI RGCGCY 1 cut(s) 459
BglI GCCNNNNNGGC 1 cut(s) 13
BisI GCNGC 4 cut(s) 81, 384, 524, 750
BlnI CCTAGG 1 cut(s) 709
BlpI GCTNAGC 1 cut(s) 309
BlsI GCNGC 4 cut(s) 82, 385, 525, 751
Bme1390I CCNGG 5 cut(s) 168, 289, 343, 393, 1060
BmeT110I CYCGRG 1 cut(s) 24
BmgT120I GGNCC 1 cut(s) 295
BmiI GGNNCC 2 cut(s) 155, 340
BmrFI CCNGG 5 cut(s) 168, 289, 343, 393, 1060
BmrI ACTGGG 2 cut(s) 736, 933
BmsI GCATC 4 cut(s) 38, 850, 951, 1022
BmuI ACTGGG 2 cut(s) 736, 933
BpiI GAAGAC 1 cut(s) 1141
BpmI CTGGAG 1 cut(s) 822
Bpu1102I GCTNAGC 1 cut(s) 309
BpuEI CTTGAG 1 cut(s) 684
BpuMI CCSGG 1 cut(s) 168
BsaBI GATNNNNATC 1 cut(s) 183
BsaHI GRCGYC 1 cut(s) 5
BsaJI CCNNGG 4 cut(s) 287, 391, 501, 709
Bsc4I CCNNNNNNNGG 4 cut(s) 557, 634, 726, 891
Bse1I ACTGG 3 cut(s) 527, 731, 928
Bse8I GATNNNNATC 1 cut(s) 183
BseBI CCWGG 4 cut(s) 289, 343, 393, 1060
BseDI CCNNGG 4 cut(s) 287, 391, 501, 709
BseGI GGATG 5 cut(s) 135, 437, 467, 749, 1055
BseJI GATNNNNATC 1 cut(s) 183
BseLI CCNNNNNNNGG 4 cut(s) 557, 634, 726, 891
BseMII CTCAG 3 cut(s) 163, 202, 973
BseNI ACTGG 3 cut(s) 527, 731, 928
BseRI GAGGAG 1 cut(s) 596
BseXI GCAGC 3 cut(s) 67, 395, 736
BseYI CCCAGC 1 cut(s) 113
Bsh1236I CGCG 1 cut(s) 34
BshFI GGCC 5 cut(s) 296, 358, 526, 697, 988
BshNI GGYRCC 2 cut(s) 153, 338
BsiHKCI CYCGRG 1 cut(s) 24
BsiSI CCGG 1 cut(s) 168
BslI CCNNNNNNNGG 4 cut(s) 557, 634, 726, 891
BsmAI GTCTC 2 cut(s) 27, 91
BsnI GGCC 5 cut(s) 296, 358, 526, 697, 988
BsoBI CYCGRG 1 cut(s) 24
Bsp1407I TGTACA 1 cut(s) 792
Bsp143I GATC 6 cut(s) 184, 270, 475, 484, 769, 859
Bsp1720I GCTNAGC 1 cut(s) 309
Bsp19I CCATGG 1 cut(s) 501
BspACI CCGC 3 cut(s) 298, 523, 885
BspANI GGCC 5 cut(s) 296, 358, 526, 697, 988
BspCNI CTCAG 3 cut(s) 162, 201, 972
BspFNI CGCG 1 cut(s) 34
BspHI TCATGA 1 cut(s) 675
BspLI GGNNCC 2 cut(s) 155, 340
BspMAI CTGCAG 1 cut(s) 85
BspPI GGATC 2 cut(s) 278, 764
BspT107I GGYRCC 2 cut(s) 153, 338
BsrGI TGTACA 1 cut(s) 792
BsrI ACTGG 3 cut(s) 527, 731, 928
BssECI CCNNGG 4 cut(s) 287, 391, 501, 709
BssMI GATC 6 cut(s) 184, 270, 475, 484, 769, 859
BssNI GRCGYC 1 cut(s) 5
BssT1I CCWWGG 2 cut(s) 501, 709
Bst2UI CCWGG 4 cut(s) 289, 343, 393, 1060
Bst4CI ACNGT 3 cut(s) 158, 469, 1045
BstACI GRCGYC 1 cut(s) 5
BstAUI TGTACA 1 cut(s) 792
BstC8I GCNNGC 6 cut(s) 294, 298, 315, 319, 351, 986
BstDEI CTNAG 4 cut(s) 149, 188, 309, 959
BstDSI CCRYGG 1 cut(s) 501
BstENI CCTNNNNNAGG 1 cut(s) 555
BstF5I GGATG 5 cut(s) 135, 437, 467, 749, 1055
BstFNI CGCG 1 cut(s) 34
BstH2I RGCGCY 1 cut(s) 459
BstHHI GCGC 3 cut(s) 202, 458, 841
BstKTI GATC 6 cut(s) 187, 273, 478, 487, 772, 862
BstMAI GTCTC 2 cut(s) 27, 91
BstMBI GATC 6 cut(s) 184, 270, 475, 484, 769, 859
BstMWI GCNNNNNNNGC 6 cut(s) 13, 46, 199, 314, 355, 1004
BstNI CCWGG 4 cut(s) 289, 343, 393, 1060
BstNSI RCATGY 1 cut(s) 367
BstSCI CCNGG 5 cut(s) 166, 287, 341, 391, 1058
BstSFI CTRYAG 1 cut(s) 81
BstUI CGCG 1 cut(s) 34
BstV1I GCAGC 3 cut(s) 67, 395, 736
BstV2I GAAGAC 1 cut(s) 1141
BsuRI GGCC 5 cut(s) 296, 358, 526, 697, 988
BtgI CCRYGG 1 cut(s) 501
BtgZI GCGATG 2 cut(s) 15, 258
BtsCI GGATG 5 cut(s) 135, 437, 467, 749, 1055
BtsI GCAGTG 1 cut(s) 1123
BtsIMutI CAGTG 2 cut(s) 967, 1123
Cac8I GCNNGC 6 cut(s) 294, 298, 315, 319, 351, 986
CciI TCATGA 1 cut(s) 675
CfoI GCGC 3 cut(s) 202, 458, 841
Cfr13I GGNCC 1 cut(s) 295
CseI GACGC 2 cut(s) 13, 284
CsiI ACCWGGT 1 cut(s) 341
Csp6I GTAC 3 cut(s) 465, 513, 793
CspCI CAANNNNNGTGG 2 cut(s) 1010, 1045
CviAII CATG 3 cut(s) 364, 502, 676
CviQI GTAC 3 cut(s) 465, 513, 793
DdeI CTNAG 4 cut(s) 149, 188, 309, 959
DpnI GATC 6 cut(s) 186, 272, 477, 486, 771, 861
DpnII GATC 6 cut(s) 184, 270, 475, 484, 769, 859
DraI TTTAAA 1 cut(s) 556
DrdI GACNNNNNNGTC 1 cut(s) 236
DseDI GACNNNNNNGTC 1 cut(s) 236
EaeI YGGCCR 2 cut(s) 524, 986
Eco130I CCWWGG 2 cut(s) 501, 709
Eco57I CTGAAG 2 cut(s) 65, 884
Eco88I CYCGRG 1 cut(s) 24
EcoNI CCTNNNNNAGG 1 cut(s) 555
EcoRI GAATTC 1 cut(s) 600
EcoRII CCWGG 4 cut(s) 287, 341, 391, 1058
EcoT14I CCWWGG 2 cut(s) 501, 709
ErhI CCWWGG 2 cut(s) 501, 709
FaeI CATG 3 cut(s) 367, 505, 679
FatI CATG 3 cut(s) 363, 501, 675
FauI CCCGC 1 cut(s) 305
Fnu4HI GCNGC 4 cut(s) 81, 384, 524, 750
FokI GGATG 5 cut(s) 122, 444, 474, 756, 1042
Fsp4HI GCNGC 4 cut(s) 81, 384, 524, 750
FspAI RTGCGCAY 1 cut(s) 840
FspBI CTAG 2 cut(s) 656, 710
FspI TGCGCA 1 cut(s) 840
GlaI GCGC 3 cut(s) 201, 457, 840
GluI GCNGC 4 cut(s) 81, 384, 524, 750
GsaI CCCAGC 1 cut(s) 117
GsuI CTGGAG 1 cut(s) 822
HaeII RGCGCY 1 cut(s) 459
HaeIII GGCC 5 cut(s) 296, 358, 526, 697, 988
HapII CCGG 1 cut(s) 168
HgaI GACGC 2 cut(s) 13, 284
HhaI GCGC 3 cut(s) 202, 458, 841
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 3 cut(s) 367, 505, 679
Hin6I GCGC 3 cut(s) 200, 456, 839
HinP1I GCGC 3 cut(s) 200, 456, 839
HincII GTYRAC 3 cut(s) 420, 687, 1048
HindII GTYRAC 3 cut(s) 420, 687, 1048
HindIII AAGCTT 1 cut(s) 261
HinfI GANTC 6 cut(s) 163, 179, 237, 902, 933, 1122
HpaII CCGG 1 cut(s) 168
HphI GGTGA 2 cut(s) 89, 911
Hpy166II GTNNAC 3 cut(s) 420, 687, 1048
Hpy188I TCNGA 3 cut(s) 45, 184, 864
Hpy188III TCNNGA 7 cut(s) 176, 404, 488, 676, 801, 845, 1017
Hpy8I GTNNAC 3 cut(s) 420, 687, 1048
Hpy99I CGWCG 1 cut(s) 278
HpyAV CCTTC 3 cut(s) 63, 410, 859
HpyCH4III ACNGT 3 cut(s) 158, 469, 1045
HpyCH4IV ACGT 1 cut(s) 511
HpyCH4V TGCA 7 cut(s) 83, 363, 752, 942, 998, 1071, 1116
HpyF10VI GCNNNNNNNGC 6 cut(s) 13, 46, 199, 314, 355, 1004
HpyF3I CTNAG 4 cut(s) 149, 188, 309, 959
HpySE526I ACGT 1 cut(s) 511
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 3 cut(s) 367, 505, 679
HspAI GCGC 3 cut(s) 200, 456, 839
Kzo9I GATC 6 cut(s) 184, 270, 475, 484, 769, 859
LmnI GCTCC 2 cut(s) 71, 731
Lsp1109I GCAGC 3 cut(s) 67, 395, 736
LweI GCATC 4 cut(s) 38, 850, 951, 1022
MabI ACCWGGT 1 cut(s) 341
MaeI CTAG 2 cut(s) 656, 710
MaeII ACGT 1 cut(s) 511
MalI GATC 6 cut(s) 186, 272, 477, 486, 771, 861
MboI GATC 6 cut(s) 184, 270, 475, 484, 769, 859
MboII GAAGA 2 cut(s) 866, 1146
MfeI CAATTG 1 cut(s) 581
MlsI TGGCCA 1 cut(s) 988
MluCI AATT 6 cut(s) 581, 600, 619, 753, 978, 1022
MluNI TGGCCA 1 cut(s) 988
MlyI GAGTC 1 cut(s) 246
MmeI TCCRAC 2 cut(s) 140, 634
MnlI CCTC 7 cut(s) 60, 332, 617, 683, 708, 885, 968
Mox20I TGGCCA 1 cut(s) 988
MroXI GAANNNNTTC 1 cut(s) 1138
MscI TGGCCA 1 cut(s) 988
MseI TTAA 6 cut(s) 144, 411, 449, 555, 723, 756
MslI CAYNNNNRTG 1 cut(s) 503
Msp20I TGGCCA 1 cut(s) 988
MspI CCGG 1 cut(s) 168
MspR9I CCNGG 5 cut(s) 168, 289, 343, 393, 1060
MunI CAATTG 1 cut(s) 581
MvaI CCWGG 4 cut(s) 289, 343, 393, 1060
MvnI CGCG 1 cut(s) 34
MwoI GCNNNNNNNGC 6 cut(s) 13, 46, 199, 314, 355, 1004
NciI CCSGG 1 cut(s) 168
NcoI CCATGG 1 cut(s) 501
NdeII GATC 6 cut(s) 184, 270, 475, 484, 769, 859
NlaIII CATG 3 cut(s) 367, 505, 679
NlaIV GGNNCC 2 cut(s) 155, 340
NsbI TGCGCA 1 cut(s) 840
NspI RCATGY 1 cut(s) 367
OliI CACNNNNGTG 1 cut(s) 503
PagI TCATGA 1 cut(s) 675
PcsI WCGNNNNNNNCGW 2 cut(s) 31, 321
PdmI GAANNNNTTC 1 cut(s) 1138
PfeI GAWTC 5 cut(s) 163, 179, 902, 933, 1122
PkrI GCNGC 4 cut(s) 82, 385, 525, 751
PleI GAGTC 1 cut(s) 245
PpsI GAGTC 1 cut(s) 245
Psp6I CCWGG 4 cut(s) 287, 341, 391, 1058
PspFI CCCAGC 1 cut(s) 113
PspGI CCWGG 4 cut(s) 287, 341, 391, 1058
PspN4I GGNNCC 2 cut(s) 155, 340
PspPI GGNCC 1 cut(s) 295
PstI CTGCAG 1 cut(s) 85
RsaI GTAC 3 cut(s) 466, 514, 794
RsaNI GTAC 3 cut(s) 465, 513, 793
RseI CAYNNNNRTG 1 cut(s) 503
SaqAI TTAA 6 cut(s) 144, 411, 449, 555, 723, 756
SatI GCNGC 4 cut(s) 81, 384, 524, 750
Sau3AI GATC 6 cut(s) 184, 270, 475, 484, 769, 859
Sau96I GGNCC 1 cut(s) 295
SchI GAGTC 1 cut(s) 246
ScrFI CCNGG 5 cut(s) 168, 289, 343, 393, 1060
SexAI ACCWGGT 1 cut(s) 341
SfaNI GCATC 4 cut(s) 38, 850, 951, 1022
SfcI CTRYAG 1 cut(s) 81
SmiMI CAYNNNNRTG 1 cut(s) 503
SmlI CTYRAG 1 cut(s) 699
SmoI CTYRAG 1 cut(s) 699
Sse9I AATT 6 cut(s) 581, 600, 619, 753, 978, 1022
SsiI CCGC 3 cut(s) 298, 523, 885
SspMI CTAG 2 cut(s) 656, 710
StyD4I CCNGG 5 cut(s) 166, 287, 341, 391, 1058
StyI CCWWGG 2 cut(s) 501, 709
TaaI ACNGT 3 cut(s) 158, 469, 1045
TaiI ACGT 1 cut(s) 514
TaqI TCGA 3 cut(s) 218, 273, 324
TasI AATT 6 cut(s) 581, 600, 619, 753, 978, 1022
TatI WGTACW 1 cut(s) 792
TauI GCSGC 1 cut(s) 526
TfiI GAWTC 5 cut(s) 163, 179, 902, 933, 1122
Tru1I TTAA 6 cut(s) 144, 411, 449, 555, 723, 756
Tru9I TTAA 6 cut(s) 144, 411, 449, 555, 723, 756
TscAI CASTG 2 cut(s) 967, 1123
TseI GCWGC 3 cut(s) 80, 383, 749
TspDTI ATGAA 5 cut(s) 124, 204, 692, 1044, 1131
TspGWI ACGGA 1 cut(s) 777
TspRI CASTG 2 cut(s) 967, 1123
XagI CCTNNNNNAGG 1 cut(s) 555
XapI RAATTY 1 cut(s) 600
XceI RCATGY 1 cut(s) 367
XmaJI CCTAGG 1 cut(s) 709
XmnI GAANNNNTTC 1 cut(s) 1138
XspI CTAG 2 cut(s) 656, 710
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.