Rroxscaffold_5G00386980

Catalyzes the prenylation of para-hydroxybenzoate (PHB) with an all-trans polyprenyl group. Mediates the second step in the final reaction sequence of coenzyme Q (CoQ) biosynthesis, which is the condensation of the polyisoprenoid side chain with PHB, generating the first membrane-bound Q intermediate

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0001284
Physical Location & Seq
Reverse (-)
65708116 .. 65709736
1621 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00386980.1

Sequence Viewer

Length: 519 bp
ATGCTTGTTGGTTTGTGCAGGTCAATAACACTGGCAGCAAGCCCAGGACACCTTCCTGATATTAAGATATTGACACTATTTGGATGTGGGGCTTTGCTTTTAAAGGGGGCCGGATGTACCATAAATGATCTCCTTGATCGGGATATTGACACCATGCCTCAAGCATACCTTGGTTTGACCTTTAACTGGGGAGCTTTGTTAGGATGGGCTGCAATCAAAGGAAGCGTTGATCCAGCTATAGTACTCCCATTGTACATTTCTGGAGTATTTTGGACACTTGTGTATGATACGATATATGCACATCAGGATAAAGAAGATGATCTGAAGGTTGGTGTCAAATCTACCGCTTTGAGGTTTGGTGATTCAACAAAGGAGTGGATTACTGGGTTGGGAATCCTGTGCATCAGTAGTCCATATTATGCATTTTTGGCAGTTGCATCTGGACAATTAGCTTGGCAGATATGGACAATTGCCCTTTCATCCAGGCTTGATTGCAATGGAAAGTATGTATCATTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00130 ko01100 ko01110 map00130 map01100 map01110
Pfam Domains
Protein Families
UbiA

Protein Analysis

172

Amino Acids

18.59

Weight (kDa)

5.53

Isoelectric Point (pI)

23.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UbiA PF01040 10 - 51 3.9e-07 UbiA prenyltransferase family
UbiA PF01040 52 - 159 2.8e-19 UbiA prenyltransferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 9
AciI CCGC 1 cut(s) 345
AclWI GGATC 1 cut(s) 224
AcuI CTGAAG 1 cut(s) 344
AfaI GTAC 3 cut(s) 118, 243, 254
AfiI CCNNNNNNNGG 3 cut(s) 139, 186, 351
AgsI TTSAA 1 cut(s) 366
AjnI CCWGG 2 cut(s) 43, 482
AluBI AGCT 3 cut(s) 194, 236, 452
AluI AGCT 3 cut(s) 194, 236, 452
AlwI GGATC 1 cut(s) 224
AoxI GGCC 1 cut(s) 108
ApeKI GCWGC 2 cut(s) 35, 209
AspS9I GGNCC 1 cut(s) 108
AsuHPI GGTGA 1 cut(s) 371
BbvI GCAGC 2 cut(s) 47, 196
BccI CCATC 1 cut(s) 198
BciT130I CCWGG 2 cut(s) 45, 484
BfmI CTRYAG 1 cut(s) 237
BfuAI ACCTGC 1 cut(s) 9
BisI GCNGC 2 cut(s) 36, 210
BlsI GCNGC 2 cut(s) 37, 211
BmcAI AGTACT 1 cut(s) 243
Bme1390I CCNGG 2 cut(s) 45, 484
BmgT120I GGNCC 1 cut(s) 108
BmiI GGNNCC 1 cut(s) 109
BmrFI CCNGG 2 cut(s) 45, 484
BmrI ACTGGG 2 cut(s) 196, 393
BmsI GCATC 2 cut(s) 411, 446
BmuI ACTGGG 2 cut(s) 196, 393
BpmI CTGGAG 1 cut(s) 282
BpuEI CTTGAG 1 cut(s) 144
BsaJI CCNNGG 2 cut(s) 43, 169
Bsc4I CCNNNNNNNGG 3 cut(s) 139, 186, 351
Bse1I ACTGG 3 cut(s) 36, 191, 388
Bse3DI GCAATG 1 cut(s) 502
BseBI CCWGG 2 cut(s) 45, 484
BseDI CCNNGG 2 cut(s) 43, 169
BseGI GGATG 4 cut(s) 89, 119, 209, 479
BseLI CCNNNNNNNGG 3 cut(s) 139, 186, 351
BseMI GCAATG 1 cut(s) 502
BseNI ACTGG 3 cut(s) 36, 191, 388
BseXI GCAGC 2 cut(s) 47, 196
BsgI GTGCAG 1 cut(s) 37
BshFI GGCC 1 cut(s) 110
BsiSI CCGG 1 cut(s) 111
BslI CCNNNNNNNGG 3 cut(s) 139, 186, 351
BsnI GGCC 1 cut(s) 110
Bsp1407I TGTACA 1 cut(s) 252
Bsp143I GATC 4 cut(s) 127, 136, 229, 319
BspACI CCGC 1 cut(s) 345
BspANI GGCC 1 cut(s) 110
BspLI GGNNCC 1 cut(s) 109
BspMI ACCTGC 1 cut(s) 9
BspPI GGATC 1 cut(s) 224
BsrDI GCAATG 1 cut(s) 502
BsrGI TGTACA 1 cut(s) 252
BsrI ACTGG 3 cut(s) 36, 191, 388
BssECI CCNNGG 2 cut(s) 43, 169
BssMI GATC 4 cut(s) 127, 136, 229, 319
BssT1I CCWWGG 1 cut(s) 169
Bst2UI CCWGG 2 cut(s) 45, 484
BstAUI TGTACA 1 cut(s) 252
BstC8I GCNNGC 1 cut(s) 40
BstF5I GGATG 4 cut(s) 89, 119, 209, 479
BstKTI GATC 4 cut(s) 130, 139, 232, 322
BstMBI GATC 4 cut(s) 127, 136, 229, 319
BstMWI GCNNNNNNNGC 1 cut(s) 428
BstNI CCWGG 2 cut(s) 45, 484
BstSCI CCNGG 2 cut(s) 43, 482
BstSFI CTRYAG 1 cut(s) 237
BstV1I GCAGC 2 cut(s) 47, 196
BsuRI GGCC 1 cut(s) 110
BtsCI GGATG 4 cut(s) 89, 119, 209, 479
BtsIMutI CAGTG 1 cut(s) 29
BveI ACCTGC 1 cut(s) 9
Cac8I GCNNGC 1 cut(s) 40
Cfr13I GGNCC 1 cut(s) 108
Csp6I GTAC 3 cut(s) 117, 242, 253
CviAII CATG 1 cut(s) 154
CviJI RGCY 8 cut(s) 42, 92, 110, 194, 209, 236, 452, 487
CviKI_1 RGCY 8 cut(s) 42, 92, 110, 194, 209, 236, 452, 487
CviQI GTAC 3 cut(s) 117, 242, 253
DpnI GATC 4 cut(s) 129, 138, 231, 321
DpnII GATC 4 cut(s) 127, 136, 229, 319
DraI TTTAAA 1 cut(s) 102
Eco130I CCWWGG 1 cut(s) 169
Eco57I CTGAAG 1 cut(s) 344
EcoRII CCWGG 2 cut(s) 43, 482
EcoT14I CCWWGG 1 cut(s) 169
EcoT22I ATGCAT 1 cut(s) 424
ErhI CCWWGG 1 cut(s) 169
FaeI CATG 1 cut(s) 157
FalI AAGNNNNNCTT 2 cut(s) 153, 185
FatI CATG 1 cut(s) 153
Fnu4HI GCNGC 2 cut(s) 36, 210
FokI GGATG 4 cut(s) 96, 126, 216, 466
Fsp4HI GCNGC 2 cut(s) 36, 210
GluI GCNGC 2 cut(s) 36, 210
GsuI CTGGAG 1 cut(s) 282
HaeIII GGCC 1 cut(s) 110
HapII CCGG 1 cut(s) 111
Hin1II CATG 1 cut(s) 157
HinfI GANTC 2 cut(s) 362, 393
HpaII CCGG 1 cut(s) 111
HphI GGTGA 1 cut(s) 371
Hpy188I TCNGA 1 cut(s) 324
Hpy188III TCNNGA 5 cut(s) 56, 140, 261, 305, 441
HpyAV CCTTC 2 cut(s) 62, 319
HpyCH4V TGCA 7 cut(s) 18, 212, 299, 402, 422, 437, 495
HpyF10VI GCNNNNNNNGC 1 cut(s) 428
Hsp92II CATG 1 cut(s) 157
Kzo9I GATC 4 cut(s) 127, 136, 229, 319
LmnI GCTCC 1 cut(s) 191
Lsp1109I GCAGC 2 cut(s) 47, 196
LweI GCATC 2 cut(s) 411, 446
MalI GATC 4 cut(s) 129, 138, 231, 321
MboI GATC 4 cut(s) 127, 136, 229, 319
MboII GAAGA 1 cut(s) 326
MfeI CAATTG 1 cut(s) 468
MluCI AATT 2 cut(s) 446, 468
MnlI CCTC 2 cut(s) 168, 345
Mph1103I ATGCAT 1 cut(s) 424
MseI TTAA 3 cut(s) 63, 101, 183
MspI CCGG 1 cut(s) 111
MspR9I CCNGG 2 cut(s) 45, 484
MunI CAATTG 1 cut(s) 468
MvaI CCWGG 2 cut(s) 45, 484
MwoI GCNNNNNNNGC 1 cut(s) 428
NdeII GATC 4 cut(s) 127, 136, 229, 319
NlaIII CATG 1 cut(s) 157
NlaIV GGNNCC 1 cut(s) 109
NsiI ATGCAT 1 cut(s) 424
PfeI GAWTC 2 cut(s) 362, 393
PkrI GCNGC 2 cut(s) 37, 211
Psp6I CCWGG 2 cut(s) 43, 482
PspGI CCWGG 2 cut(s) 43, 482
PspN4I GGNNCC 1 cut(s) 109
PspPI GGNCC 1 cut(s) 108
RsaI GTAC 3 cut(s) 118, 243, 254
RsaNI GTAC 3 cut(s) 117, 242, 253
SaqAI TTAA 3 cut(s) 63, 101, 183
SatI GCNGC 2 cut(s) 36, 210
Sau3AI GATC 4 cut(s) 127, 136, 229, 319
Sau96I GGNCC 1 cut(s) 108
ScaI AGTACT 1 cut(s) 243
ScrFI CCNGG 2 cut(s) 45, 484
SetI ASST 9 cut(s) 23, 54, 171, 182, 196, 238, 330, 356, 454
SfaNI GCATC 2 cut(s) 411, 446
SfcI CTRYAG 1 cut(s) 237
SmlI CTYRAG 1 cut(s) 159
SmoI CTYRAG 1 cut(s) 159
Sse9I AATT 2 cut(s) 446, 468
SsiI CCGC 1 cut(s) 345
StyD4I CCNGG 2 cut(s) 43, 482
StyI CCWWGG 1 cut(s) 169
TasI AATT 2 cut(s) 446, 468
TatI WGTACW 2 cut(s) 241, 252
TfiI GAWTC 2 cut(s) 362, 393
Tru1I TTAA 3 cut(s) 63, 101, 183
Tru9I TTAA 3 cut(s) 63, 101, 183
TscAI CASTG 1 cut(s) 36
TseI GCWGC 2 cut(s) 35, 209
TspDTI ATGAA 1 cut(s) 468
TspRI CASTG 1 cut(s) 36
ZrmI AGTACT 1 cut(s) 243
Zsp2I ATGCAT 1 cut(s) 424
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.