Rorug05G0219400

Catalyzes the prenylation of para-hydroxybenzoate (PHB) with an all-trans polyprenyl group. Mediates the second step in the final reaction sequence of coenzyme Q (CoQ) biosynthesis, which is the condensation of the polyisoprenoid side chain with PHB, generating the first membrane-bound Q intermediate

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0001284
Physical Location & Seq
Reverse (-)
22595701 .. 22610379
14679 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0219400.1

Sequence Viewer

Length: 1362 bp
ATGTCTCTTAGTCCTTCGCTACAGCATTGGCGCGATTCAAAGAAATACAGTGGCATTGTTGAGGACTGCTGTTGTGATTATGAAACGGTGGACAGTGTTAATGCAGAAGTGTTGTATCCTCTGCTACAAGAGATTGTTAAAACTCCGTTTTTTCGATATTTTAAGGCCAACCTGTGGTGTGACTGTCCCTTCTGGACTGAAGATGGTATGTGCCTCCTGCGTGACTGCAGCGTCTGCGAATGCCCAGAAAATGAATTTCCGGAACCTTTCAAGAGGCCCTTTCACCGTGGCCTTCCATCCGACAGTTTGGTTTGTCAAGATGATAAGCTGCAGGGGTCTGTTGACCGCACTCTAGACAGTAGAGCATTCAGAGGCTGGATTGAGACAGATAATCCTTGGACAAATGATGACGAGACTGATAATGATGAGATGACATATGTGAACCTTCTACTGAACCCTGAACGTTACACTGGCTATACTGGTCCATCAGCAAGAAGGATATGGGATGCTGTTTATTCTGAAAATTGCCCAAAATATTCATCTCAGGACATTTTCCAGGAGCAAAGAGTATTGTACAAATTAATATCGGGTCTTCACTCATCAATTTCAATCCACATAGCTGCTGATTATCTCCTAGACAAGATTACCAATCTGTGGGGTCAAAACATGGAATTGATGCATGATCGTGTTTTAAAATATCCAGATCGTGTTCAGAATTTGTACTTCGCATTCCTCTTTGTTCTCCGGGCAGTGACAAAAGCTGCAGATTACTTAGAGCAGGCTGAGTATGATACAGGTAATCATGAGGAAGACCTGAGAACAGAGTCTTTGATGAGGCAGCTACTTTACAATCCTAAACTCCAAGCTGCATGTCCCCTACCGTTTGATGAAGCAAAGCTCTGGAAAGGTCAGAGCGGACCCCAATTGAAGCAACAAATTCAAAAGAAATTCAGAAACATTAGTGCATTAATGGATTGTGTGGGATGTGAGAAATGTCGGCTCTGGGGAAAGCTTCAAGTTCTTGGTCTTGGTACTGCATTGAAGATCCTCTTTTCTGTTGATGGTAAAAATCATCAAGATCAACCTCTGCAGCTGCAACGAAATGAAGTGATAGCTCTCGTAAATCTGCTAAATCGACTCTCTGAATCTGTCAAATATGCTTGTCAAAGCGGACATTCAATCAAGAAAGTAGCTGAAGGAGGACGAGTTGTTGAACGTTATTCTATACAAACAATCAGTACATTGCAAAGAATATGGGAGGCGGTGATCCCGATCAGGCTTAGGTTAGGGATGGATGTTAGCACTGTAACTATTGGACATCACAATTCTAGAGCAAATGTAAAGCATACACTGAGTCACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00130 ko01100 ko01110 map00130 map01100 map01110
Pfam Domains
Protein Families
UbiA

Protein Analysis

453

Amino Acids

52.18

Weight (kDa)

5.99

Isoelectric Point (pI)

46.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ERO1 PF04137 38 - 386 3.1e-121 Endoplasmic Reticulum Oxidoreductin 1 (ERO1)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 230
AccB7I CCANNNNNTGG 2 cut(s) 174, 654
AccBSI CCGCTC 1 cut(s) 915
AccII CGCG 1 cut(s) 33
AccIII TCCGGA 1 cut(s) 259
AciI CCGC 4 cut(s) 346, 915, 1170, 1262
AclI AACGTT 2 cut(s) 463, 1216
AclWI GGATC 2 cut(s) 1039, 1261
AcsI RAATTY 4 cut(s) 254, 715, 936, 947
AcuI CTGAAG 2 cut(s) 219, 1215
AfaI GTAC 4 cut(s) 575, 722, 1033, 1240
AfiI CCNNNNNNNGG 2 cut(s) 174, 654
AgsI TTSAA 9 cut(s) 39, 271, 609, 928, 941, 1016, 1042, 1179, 1214
AjnI CCWGG 1 cut(s) 555
Alw26I GTCTC 3 cut(s) 9, 377, 407
AlwI GGATC 2 cut(s) 1039, 1261
AlwNI CAGNNNCTG 2 cut(s) 234, 375
Aor13HI TCCGGA 1 cut(s) 259
AoxI GGCC 3 cut(s) 165, 275, 289
ApeKI GCWGC 8 cut(s) 228, 328, 620, 761, 838, 866, 1090, 1093
ApoI RAATTY 4 cut(s) 254, 715, 936, 947
AseI ATTAAT 2 cut(s) 581, 968
AspLEI GCGC 1 cut(s) 33
AspS9I GGNCC 3 cut(s) 276, 482, 917
AsuC2I CCSGG 1 cut(s) 746
AsuHPI GGTGA 2 cut(s) 275, 1276
AvaII GGWCC 2 cut(s) 482, 917
BbsI GAAGAC 2 cut(s) 584, 816
BbvI GCAGC 8 cut(s) 240, 315, 607, 748, 850, 853, 1080, 1102
BccI CCATC 5 cut(s) 197, 304, 493, 1055, 1285
BciT130I CCWGG 1 cut(s) 557
BciVI GTATCC 1 cut(s) 126
BcnI CCSGG 1 cut(s) 746
BcoDI GTCTC 3 cut(s) 9, 377, 407
BfaI CTAG 3 cut(s) 353, 635, 1329
BfmI CTRYAG 5 cut(s) 20, 226, 329, 762, 1088
BfuI GTATCC 1 cut(s) 126
BisI GCNGC 8 cut(s) 229, 329, 621, 762, 839, 867, 1091, 1094
BlsI GCNGC 8 cut(s) 230, 330, 622, 763, 840, 868, 1092, 1095
Bme1390I CCNGG 2 cut(s) 557, 746
Bme18I GGWCC 2 cut(s) 482, 917
BmgT120I GGNCC 3 cut(s) 276, 482, 917
BmiI GGNNCC 2 cut(s) 264, 919
BmrFI CCNGG 2 cut(s) 557, 746
BmsI GCATC 2 cut(s) 496, 666
BpiI GAAGAC 2 cut(s) 584, 816
Bpu10I CCTNAGC 1 cut(s) 1280
BpuMI CCSGG 1 cut(s) 746
BsaBI GATNNNNATC 1 cut(s) 1271
BsaJI CCNNGG 2 cut(s) 286, 395
BsaWI WCCGGW 1 cut(s) 259
BsaXI ACNNNNNCTCC 2 cut(s) 1191, 1221
Bsc4I CCNNNNNNNGG 2 cut(s) 174, 654
Bse1I ACTGG 2 cut(s) 475, 484
Bse3DI GCAATG 1 cut(s) 1241
Bse8I GATNNNNATC 1 cut(s) 1271
BseAI TCCGGA 1 cut(s) 259
BseBI CCWGG 1 cut(s) 557
BseDI CCNNGG 2 cut(s) 286, 395
BseGI GGATG 5 cut(s) 296, 511, 989, 1296, 1300
BseJI GATNNNNATC 1 cut(s) 1271
BseLI CCNNNNNNNGG 2 cut(s) 174, 654
BseMI GCAATG 1 cut(s) 1241
BseMII CTCAG 4 cut(s) 557, 774, 806, 1343
BseNI ACTGG 2 cut(s) 475, 484
BseXI GCAGC 8 cut(s) 240, 315, 607, 748, 850, 853, 1080, 1102
Bsh1236I CGCG 1 cut(s) 33
BshFI GGCC 3 cut(s) 167, 277, 291
BsiSI CCGG 2 cut(s) 260, 745
BslFI GGGAC 2 cut(s) 171, 858
BslI CCNNNNNNNGG 2 cut(s) 174, 654
BsmAI GTCTC 3 cut(s) 9, 377, 407
BsmFI GGGAC 2 cut(s) 171, 858
BsmI GAATGC 3 cut(s) 245, 365, 728
BsnI GGCC 3 cut(s) 167, 277, 291
Bsp13I TCCGGA 1 cut(s) 259
Bsp1407I TGTACA 1 cut(s) 573
Bsp143I GATC 6 cut(s) 682, 703, 1044, 1078, 1266, 1272
BspACI CCGC 4 cut(s) 346, 915, 1170, 1262
BspANI GGCC 3 cut(s) 167, 277, 291
BspCNI CTCAG 4 cut(s) 556, 775, 807, 1344
BspEI TCCGGA 1 cut(s) 259
BspFNI CGCG 1 cut(s) 33
BspHI TCATGA 1 cut(s) 802
BspLI GGNNCC 2 cut(s) 264, 919
BspMAI CTGCAG 4 cut(s) 230, 333, 766, 1092
BspPI GGATC 2 cut(s) 1039, 1261
BsrBI CCGCTC 1 cut(s) 915
BsrDI GCAATG 1 cut(s) 1241
BsrGI TGTACA 1 cut(s) 573
BsrI ACTGG 2 cut(s) 475, 484
BssECI CCNNGG 2 cut(s) 286, 395
BssMI GATC 6 cut(s) 682, 703, 1044, 1078, 1266, 1272
BssT1I CCWWGG 1 cut(s) 395
Bst2UI CCWGG 1 cut(s) 557
Bst4CI ACNGT 9 cut(s) 50, 88, 95, 185, 287, 305, 359, 882, 1306
BstAPI GCANNNNNTGC 1 cut(s) 234
BstAUI TGTACA 1 cut(s) 573
BstC8I GCNNGC 1 cut(s) 780
BstDEI CTNAG 7 cut(s) 8, 543, 772, 783, 815, 1280, 1352
BstDSI CCRYGG 1 cut(s) 286
BstF5I GGATG 5 cut(s) 296, 511, 989, 1296, 1300
BstFNI CGCG 1 cut(s) 33
BstHHI GCGC 1 cut(s) 33
BstKTI GATC 6 cut(s) 685, 706, 1047, 1081, 1269, 1275
BstMAI GTCTC 3 cut(s) 9, 377, 407
BstMBI GATC 6 cut(s) 682, 703, 1044, 1078, 1266, 1272
BstMWI GCNNNNNNNGC 1 cut(s) 234
BstNI CCWGG 1 cut(s) 557
BstNSI RCATGY 1 cut(s) 873
BstSCI CCNGG 2 cut(s) 555, 744
BstSFI CTRYAG 5 cut(s) 20, 226, 329, 762, 1088
BstUI CGCG 1 cut(s) 33
BstV1I GCAGC 8 cut(s) 240, 315, 607, 748, 850, 853, 1080, 1102
BstV2I GAAGAC 2 cut(s) 584, 816
BstX2I RGATCY 1 cut(s) 1044
BstYI RGATCY 1 cut(s) 1044
BsuI GTATCC 1 cut(s) 126
BsuRI GGCC 3 cut(s) 167, 277, 291
BtgI CCRYGG 1 cut(s) 286
BtsCI GGATG 5 cut(s) 296, 511, 989, 1296, 1300
BtsI GCAGTG 1 cut(s) 756
BtsIMutI CAGTG 7 cut(s) 55, 100, 468, 756, 1302, 1349, 1357
Cac8I GCNNGC 1 cut(s) 780
CaiI CAGNNNCTG 2 cut(s) 234, 375
CciI TCATGA 1 cut(s) 802
CfoI GCGC 1 cut(s) 33
Cfr13I GGNCC 3 cut(s) 276, 482, 917
CseI GACGC 1 cut(s) 220
Csp6I GTAC 4 cut(s) 574, 721, 1032, 1239
CviAII CATG 4 cut(s) 667, 680, 803, 870
CviQI GTAC 4 cut(s) 574, 721, 1032, 1239
DdeI CTNAG 7 cut(s) 8, 543, 772, 783, 815, 1280, 1352
DpnI GATC 6 cut(s) 684, 705, 1046, 1080, 1268, 1274
DpnII GATC 6 cut(s) 682, 703, 1044, 1078, 1266, 1272
DraI TTTAAA 1 cut(s) 693
DrdI GACNNNNNNGTC 1 cut(s) 230
DseDI GACNNNNNNGTC 1 cut(s) 230
Eco130I CCWWGG 1 cut(s) 395
Eco47I GGWCC 2 cut(s) 482, 917
Eco57I CTGAAG 2 cut(s) 219, 1215
EcoO109I RGGNCCY 1 cut(s) 276
EcoRII CCWGG 1 cut(s) 555
EcoT14I CCWWGG 1 cut(s) 395
EcoT22I ATGCAT 1 cut(s) 681
ErhI CCWWGG 1 cut(s) 395
FaeI CATG 4 cut(s) 670, 683, 806, 873
FalI AAGNNNNNCTT 4 cut(s) 263, 295, 1034, 1066
FaqI GGGAC 2 cut(s) 171, 858
FatI CATG 4 cut(s) 666, 679, 802, 869
FauNDI CATATG 1 cut(s) 436
Fnu4HI GCNGC 8 cut(s) 229, 329, 621, 762, 839, 867, 1091, 1094
FokI GGATG 5 cut(s) 283, 518, 996, 1303, 1307
Fsp4HI GCNGC 8 cut(s) 229, 329, 621, 762, 839, 867, 1091, 1094
FspBI CTAG 3 cut(s) 353, 635, 1329
GlaI GCGC 1 cut(s) 32
GluI GCNGC 8 cut(s) 229, 329, 621, 762, 839, 867, 1091, 1094
HaeIII GGCC 3 cut(s) 167, 277, 291
HapII CCGG 2 cut(s) 260, 745
HgaI GACGC 1 cut(s) 220
HhaI GCGC 1 cut(s) 33
Hin1II CATG 4 cut(s) 670, 683, 806, 873
Hin6I GCGC 1 cut(s) 31
HinP1I GCGC 1 cut(s) 31
HincII GTYRAC 1 cut(s) 343
HindII GTYRAC 1 cut(s) 343
HindIII AAGCTT 1 cut(s) 1010
HinfI GANTC 5 cut(s) 35, 824, 1137, 1145, 1354
HpaII CCGG 2 cut(s) 260, 745
HphI GGTGA 2 cut(s) 275, 1276
Hpy166II GTNNAC 3 cut(s) 91, 343, 442
Hpy188I TCNGA 7 cut(s) 301, 371, 520, 714, 912, 953, 1144
Hpy8I GTNNAC 3 cut(s) 91, 343, 442
HpyAV CCTTC 6 cut(s) 24, 199, 302, 455, 489, 1190
HpyCH4III ACNGT 9 cut(s) 50, 88, 95, 185, 287, 305, 359, 882, 1306
HpyCH4IV ACGT 2 cut(s) 463, 1216
HpyF10VI GCNNNNNNNGC 1 cut(s) 234
HpyF3I CTNAG 7 cut(s) 8, 543, 772, 783, 815, 1280, 1352
HpySE526I ACGT 2 cut(s) 463, 1216
Hsp92II CATG 4 cut(s) 670, 683, 806, 873
HspAI GCGC 1 cut(s) 31
Kpn2I TCCGGA 1 cut(s) 259
Kzo9I GATC 6 cut(s) 682, 703, 1044, 1078, 1266, 1272
LmnI GCTCC 1 cut(s) 559
Lsp1109I GCAGC 8 cut(s) 240, 315, 607, 748, 850, 853, 1080, 1102
LweI GCATC 2 cut(s) 496, 666
MaeI CTAG 3 cut(s) 353, 635, 1329
MaeII ACGT 2 cut(s) 463, 1216
MaeIII GTNAC 6 cut(s) 179, 221, 464, 751, 1306, 1355
MalI GATC 6 cut(s) 684, 705, 1046, 1080, 1268, 1274
MbiI CCGCTC 1 cut(s) 915
MboI GATC 6 cut(s) 682, 703, 1044, 1078, 1266, 1272
MboII GAAGA 4 cut(s) 212, 584, 821, 1054
MfeI CAATTG 1 cut(s) 923
MflI RGATCY 1 cut(s) 1044
MlyI GAGTC 2 cut(s) 833, 1131
MmeI TCCRAC 1 cut(s) 324
Mph1103I ATGCAT 1 cut(s) 681
MroI TCCGGA 1 cut(s) 259
MseI TTAA 6 cut(s) 99, 138, 162, 581, 692, 968
MslI CAYNNNNRTG 1 cut(s) 684
MspA1I CMGCKG 1 cut(s) 1093
MspI CCGG 2 cut(s) 260, 745
MspR9I CCNGG 2 cut(s) 557, 746
MunI CAATTG 1 cut(s) 923
Mva1269I GAATGC 3 cut(s) 245, 365, 728
MvaI CCWGG 1 cut(s) 557
MvnI CGCG 1 cut(s) 33
MwoI GCNNNNNNNGC 1 cut(s) 234
NciI CCSGG 1 cut(s) 746
NdeI CATATG 1 cut(s) 436
NdeII GATC 6 cut(s) 682, 703, 1044, 1078, 1266, 1272
NlaIII CATG 4 cut(s) 670, 683, 806, 873
NlaIV GGNNCC 2 cut(s) 264, 919
NmuCI GTSAC 4 cut(s) 179, 221, 751, 1355
NsiI ATGCAT 1 cut(s) 681
NspI RCATGY 1 cut(s) 873
PagI TCATGA 1 cut(s) 802
PctI GAATGC 3 cut(s) 245, 365, 728
PfeI GAWTC 2 cut(s) 35, 1145
PflMI CCANNNNNTGG 2 cut(s) 174, 654
PfoI TCCNGGA 1 cut(s) 555
PkrI GCNGC 8 cut(s) 230, 330, 622, 763, 840, 868, 1092, 1095
PleI GAGTC 2 cut(s) 832, 1131
PpsI GAGTC 2 cut(s) 832, 1131
PshBI ATTAAT 2 cut(s) 581, 968
Psp1406I AACGTT 2 cut(s) 463, 1216
Psp6I CCWGG 1 cut(s) 555
PspGI CCWGG 1 cut(s) 555
PspN4I GGNNCC 2 cut(s) 264, 919
PspPI GGNCC 3 cut(s) 276, 482, 917
PstI CTGCAG 4 cut(s) 230, 333, 766, 1092
PstNI CAGNNNCTG 2 cut(s) 234, 375
PsuI RGATCY 1 cut(s) 1044
PvuII CAGCTG 1 cut(s) 1093
RsaI GTAC 4 cut(s) 575, 722, 1033, 1240
RsaNI GTAC 4 cut(s) 574, 721, 1032, 1239
RseI CAYNNNNRTG 1 cut(s) 684
SaqAI TTAA 6 cut(s) 99, 138, 162, 581, 692, 968
SatI GCNGC 8 cut(s) 229, 329, 621, 762, 839, 867, 1091, 1094
Sau3AI GATC 6 cut(s) 682, 703, 1044, 1078, 1266, 1272
Sau96I GGNCC 3 cut(s) 276, 482, 917
SchI GAGTC 2 cut(s) 833, 1131
ScrFI CCNGG 2 cut(s) 557, 746
SfaNI GCATC 2 cut(s) 496, 666
SfcI CTRYAG 5 cut(s) 20, 226, 329, 762, 1088
SinI GGWCC 2 cut(s) 482, 917
SmiMI CAYNNNNRTG 1 cut(s) 684
SsiI CCGC 4 cut(s) 346, 915, 1170, 1262
SspI AATATT 1 cut(s) 536
SspMI CTAG 3 cut(s) 353, 635, 1329
StyD4I CCNGG 2 cut(s) 555, 744
StyI CCWWGG 1 cut(s) 395
TaaI ACNGT 9 cut(s) 50, 88, 95, 185, 287, 305, 359, 882, 1306
TaiI ACGT 2 cut(s) 466, 1219
TaqI TCGA 2 cut(s) 154, 1135
TatI WGTACW 3 cut(s) 573, 720, 1238
TfiI GAWTC 2 cut(s) 35, 1145
Tru1I TTAA 6 cut(s) 99, 138, 162, 581, 692, 968
Tru9I TTAA 6 cut(s) 99, 138, 162, 581, 692, 968
TscAI CASTG 6 cut(s) 55, 100, 475, 756, 1309, 1356
TseFI GTSAC 4 cut(s) 179, 221, 751, 1355
TseI GCWGC 8 cut(s) 228, 328, 620, 761, 838, 866, 1090, 1093
Tsp45I GTSAC 4 cut(s) 179, 221, 751, 1355
TspDTI ATGAA 5 cut(s) 96, 267, 528, 903, 1119
TspGWI ACGGA 1 cut(s) 135
TspRI CASTG 6 cut(s) 55, 100, 475, 756, 1309, 1356
Van91I CCANNNNNTGG 2 cut(s) 174, 654
VpaK11BI GGWCC 2 cut(s) 482, 917
VspI ATTAAT 2 cut(s) 581, 968
XapI RAATTY 4 cut(s) 254, 715, 936, 947
XbaI TCTAGA 2 cut(s) 352, 1328
XceI RCATGY 1 cut(s) 873
XspI CTAG 3 cut(s) 353, 635, 1329
Zsp2I ATGCAT 1 cut(s) 681
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.