Rw5G027950

Catalyzes the prenylation of para-hydroxybenzoate (PHB) with an all-trans polyprenyl group. Mediates the second step in the final reaction sequence of coenzyme Q (CoQ) biosynthesis, which is the condensation of the polyisoprenoid side chain with PHB, generating the first membrane-bound Q intermediate

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0001284
Physical Location & Seq
Reverse (-)
41661837 .. 41665927
4091 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G027950.1

Sequence Viewer

Length: 1152 bp
ATGACGTCGTTTCGGCTCTTGTCAGGGGCATTGCGTAGGCTTCTGAAGCCTCCTTCACTCTCTCGCTCCCATCTCTCTGCTGCAGTCTCTACCTATCCCCCAACCACCAAACCCAGCCCTAAAGAAACGCCTTTCATTCCATTTCATCTCAGGCACCGTTGGATTCCCAGTTATCATCTTGATTCTGATCCCAGGCTCGCAGCCCACATATGCACTTCATCGAACAAGGACGACAATCGGGTCAGCGATGACCCGAAAGCCCAAGCTTCTTGGATCGACGCTTATTTGCCTACGCAGGCCCGCCCTTATGCTAAGCTGGCTCGCCTCGACAAGCCCATTGGCACCTGGTTGCTTGCTTGGCCTTGTATGTGGTCAATAACATTGGCAGCAAGTCCAGGACACCTTCCTGATATTAAGATGTTGGCACTACTTGGATGTTGGGCTTTTTCTTTAAGAGGCGCCGCATGTACCATAAATGATATCCTTGATCGAGATATTGACGCCATGGTGGAACGTACAAAGTTGCGGCCAATTGCGAGTGGTCTTTTAACACCTTTTCAAGGGTTGTGTTTTCTCGGATTTCAATTGCTTTTTGTTCTTGGAACTCTCCTCCAACTGAATAATTATAGCCGTGTTTTGGGGGCTTCGTCTTTGTTGCTATTTTTCTCCTATCCCCTCATGAAAAGGTTGACATTCTGGCCTCAAGCATACCTAGGTTTGACCTTTAACTGGGGAGCTTTGTTAGGATGGGCTGCAATTAAAGGGAGCATTGATCCAGCTATAGTACTTCCATTGTACATTTCTGGAGTATTTTGGACACTTGTGTATGATACGATATATGCACACCAGGATAAAGAAGATGATCTGAAGATTGGTGTCAAATCTACCGCTTTGAAGTTTGGTGATTCAACAAAGGAGTGGATTACTGGGTTTGGAATCCTGTGCATCAGTAGTCTTGCCCTCAGTGGATATATTGCCGAAATTGGCTGGCCATATTATGCACTTCTGGCAGTTGCATCTGGACAATTAGCTTGGCAGATATGGACGGTTGACCTTTCATCTAGGCTTGATTGCAATAGAAAGTTTGTGTCGAACAAGTGGTTTGGTGCTATTATTTGCAGTGGAATCTTATTGGGAAGACTTTCATCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00130 ko01100 ko01110 map00130 map01100 map01110
Pfam Domains
Protein Families
UbiA

Protein Analysis

383

Amino Acids

42.59

Weight (kDa)

9.26

Isoelectric Point (pI)

37.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UbiA PF01040 119 - 364 7.1e-58 UbiA prenyltransferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 239
AatII GACGTC 1 cut(s) 8
AccB1I GGYRCC 3 cut(s) 153, 341, 458
AciI CCGC 4 cut(s) 301, 462, 526, 888
AclWI GGATC 3 cut(s) 182, 281, 767
AcoI YGGCCR 2 cut(s) 527, 989
AcuI CTGAAG 2 cut(s) 65, 887
AcyI GRCGYC 3 cut(s) 5, 459, 501
AfaI GTAC 4 cut(s) 469, 517, 786, 797
AfiI CCNNNNNNNGG 3 cut(s) 560, 637, 729
AgsI TTSAA 4 cut(s) 560, 584, 895, 909
AjnI CCWGG 4 cut(s) 191, 344, 394, 846
AluBI AGCT 5 cut(s) 266, 316, 737, 779, 1031
AluI AGCT 5 cut(s) 266, 316, 737, 779, 1031
Alw26I GTCTC 1 cut(s) 91
AlwI GGATC 3 cut(s) 182, 281, 767
AoxI GGCC 5 cut(s) 297, 359, 527, 698, 989
ApeKI GCWGC 4 cut(s) 80, 200, 386, 752
Asp700I GAANNNNTTC 1 cut(s) 1141
AspA2I CCTAGG 1 cut(s) 712
AspLEI GCGC 1 cut(s) 461
AspS9I GGNCC 1 cut(s) 298
AsuHPI GGTGA 1 cut(s) 914
AvrII CCTAGG 1 cut(s) 712
BalI TGGCCA 1 cut(s) 991
BanI GGYRCC 3 cut(s) 153, 341, 458
BbsI GAAGAC 1 cut(s) 1144
BbvI GCAGC 4 cut(s) 67, 212, 398, 739
BccI CCATC 2 cut(s) 78, 741
BceAI ACGGC 1 cut(s) 615
BciT130I CCWGG 4 cut(s) 193, 346, 396, 848
BcoDI GTCTC 1 cut(s) 91
BfaI CTAG 2 cut(s) 713, 1062
BfmI CTRYAG 2 cut(s) 81, 780
BfoI RGCGCY 1 cut(s) 462
BisI GCNGC 6 cut(s) 81, 201, 387, 462, 527, 753
BlnI CCTAGG 1 cut(s) 712
BlpI GCTNAGC 1 cut(s) 312
BlsI GCNGC 6 cut(s) 82, 202, 388, 463, 528, 754
BmcAI AGTACT 1 cut(s) 786
Bme1390I CCNGG 4 cut(s) 193, 346, 396, 848
BmgT120I GGNCC 1 cut(s) 298
BmiI GGNNCC 3 cut(s) 155, 343, 460
BmrFI CCNGG 4 cut(s) 193, 346, 396, 848
BmrI ACTGGG 3 cut(s) 162, 739, 936
BmsI GCATC 2 cut(s) 954, 1025
BmuI ACTGGG 3 cut(s) 162, 739, 936
BpiI GAAGAC 1 cut(s) 1144
BpmI CTGGAG 1 cut(s) 825
Bpu1102I GCTNAGC 1 cut(s) 312
BpuEI CTTGAG 1 cut(s) 687
BsaBI GATNNNNATC 1 cut(s) 186
BsaHI GRCGYC 3 cut(s) 5, 459, 501
BsaJI CCNNGG 3 cut(s) 191, 504, 712
Bsc4I CCNNNNNNNGG 3 cut(s) 560, 637, 729
Bse1I ACTGG 3 cut(s) 168, 734, 931
Bse3DI GCAATG 1 cut(s) 29
Bse8I GATNNNNATC 1 cut(s) 186
BseBI CCWGG 4 cut(s) 193, 346, 396, 848
BseDI CCNNGG 3 cut(s) 191, 504, 712
BseGI GGATG 2 cut(s) 440, 752
BseJI GATNNNNATC 1 cut(s) 186
BseLI CCNNNNNNNGG 3 cut(s) 560, 637, 729
BseMI GCAATG 1 cut(s) 29
BseMII CTCAG 2 cut(s) 163, 976
BseNI ACTGG 3 cut(s) 168, 734, 931
BseRI GAGGAG 1 cut(s) 599
BseXI GCAGC 4 cut(s) 67, 212, 398, 739
BseYI CCCAGC 1 cut(s) 113
BshFI GGCC 5 cut(s) 299, 361, 529, 700, 991
BshNI GGYRCC 3 cut(s) 153, 341, 458
BslI CCNNNNNNNGG 3 cut(s) 560, 637, 729
BsmAI GTCTC 1 cut(s) 91
BsnI GGCC 5 cut(s) 299, 361, 529, 700, 991
Bsp1407I TGTACA 1 cut(s) 795
Bsp143I GATC 5 cut(s) 187, 273, 487, 772, 862
Bsp1720I GCTNAGC 1 cut(s) 312
Bsp19I CCATGG 1 cut(s) 504
BspACI CCGC 4 cut(s) 301, 462, 526, 888
BspANI GGCC 5 cut(s) 299, 361, 529, 700, 991
BspCNI CTCAG 2 cut(s) 162, 975
BspHI TCATGA 1 cut(s) 678
BspLI GGNNCC 3 cut(s) 155, 343, 460
BspMAI CTGCAG 1 cut(s) 85
BspPI GGATC 3 cut(s) 182, 281, 767
BspT107I GGYRCC 3 cut(s) 153, 341, 458
BsrDI GCAATG 1 cut(s) 29
BsrGI TGTACA 1 cut(s) 795
BsrI ACTGG 3 cut(s) 168, 734, 931
BssECI CCNNGG 3 cut(s) 191, 504, 712
BssMI GATC 5 cut(s) 187, 273, 487, 772, 862
BssNI GRCGYC 3 cut(s) 5, 459, 501
BssT1I CCWWGG 2 cut(s) 504, 712
Bst2UI CCWGG 4 cut(s) 193, 346, 396, 848
Bst4CI ACNGT 2 cut(s) 158, 1048
BstACI GRCGYC 3 cut(s) 5, 459, 501
BstAUI TGTACA 1 cut(s) 795
BstC8I GCNNGC 7 cut(s) 198, 297, 301, 318, 322, 354, 989
BstDEI CTNAG 3 cut(s) 149, 312, 962
BstDSI CCRYGG 1 cut(s) 504
BstENI CCTNNNNNAGG 1 cut(s) 558
BstF5I GGATG 2 cut(s) 440, 752
BstH2I RGCGCY 1 cut(s) 462
BstHHI GCGC 1 cut(s) 461
BstKTI GATC 5 cut(s) 190, 276, 490, 775, 865
BstMAI GTCTC 1 cut(s) 91
BstMBI GATC 5 cut(s) 187, 273, 487, 772, 862
BstMWI GCNNNNNNNGC 4 cut(s) 46, 317, 358, 1007
BstNI CCWGG 4 cut(s) 193, 346, 396, 848
BstNSI RCATGY 1 cut(s) 468
BstSCI CCNGG 4 cut(s) 191, 344, 394, 846
BstSFI CTRYAG 2 cut(s) 81, 780
BstV1I GCAGC 4 cut(s) 67, 212, 398, 739
BstV2I GAAGAC 1 cut(s) 1144
BsuRI GGCC 5 cut(s) 299, 361, 529, 700, 991
BtgI CCRYGG 1 cut(s) 504
BtgZI GCGATG 1 cut(s) 261
BtsCI GGATG 2 cut(s) 440, 752
BtsI GCAGTG 1 cut(s) 1126
BtsIMutI CAGTG 2 cut(s) 970, 1126
Cac8I GCNNGC 7 cut(s) 198, 297, 301, 318, 322, 354, 989
CciI TCATGA 1 cut(s) 678
CfoI GCGC 1 cut(s) 461
Cfr13I GGNCC 1 cut(s) 298
CseI GACGC 2 cut(s) 287, 509
CsiI ACCWGGT 1 cut(s) 344
Csp6I GTAC 4 cut(s) 468, 516, 785, 796
CviAII CATG 3 cut(s) 465, 505, 679
CviQI GTAC 4 cut(s) 468, 516, 785, 796
DdeI CTNAG 3 cut(s) 149, 312, 962
DinI GGCGCC 1 cut(s) 460
DpnI GATC 5 cut(s) 189, 275, 489, 774, 864
DpnII GATC 5 cut(s) 187, 273, 487, 772, 862
DrdI GACNNNNNNGTC 1 cut(s) 239
DseDI GACNNNNNNGTC 1 cut(s) 239
EaeI YGGCCR 2 cut(s) 527, 989
Eco130I CCWWGG 2 cut(s) 504, 712
Eco32I GATATC 1 cut(s) 481
Eco57I CTGAAG 2 cut(s) 65, 887
EcoNI CCTNNNNNAGG 1 cut(s) 558
EcoRII CCWGG 4 cut(s) 191, 344, 394, 846
EcoRV GATATC 1 cut(s) 481
EcoT14I CCWWGG 2 cut(s) 504, 712
EgeI GGCGCC 1 cut(s) 460
EheI GGCGCC 1 cut(s) 460
ErhI CCWWGG 2 cut(s) 504, 712
FaeI CATG 3 cut(s) 468, 508, 682
FatI CATG 3 cut(s) 464, 504, 678
FauI CCCGC 1 cut(s) 308
FauNDI CATATG 1 cut(s) 209
Fnu4HI GCNGC 6 cut(s) 81, 201, 387, 462, 527, 753
FokI GGATG 2 cut(s) 447, 759
Fsp4HI GCNGC 6 cut(s) 81, 201, 387, 462, 527, 753
FspBI CTAG 2 cut(s) 713, 1062
GlaI GCGC 1 cut(s) 460
GluI GCNGC 6 cut(s) 81, 201, 387, 462, 527, 753
GsaI CCCAGC 1 cut(s) 117
GsuI CTGGAG 1 cut(s) 825
HaeII RGCGCY 1 cut(s) 462
HaeIII GGCC 5 cut(s) 299, 361, 529, 700, 991
HgaI GACGC 2 cut(s) 287, 509
HhaI GCGC 1 cut(s) 461
Hin1I GRCGYC 3 cut(s) 5, 459, 501
Hin1II CATG 3 cut(s) 468, 508, 682
Hin6I GCGC 1 cut(s) 459
HinP1I GCGC 1 cut(s) 459
HincII GTYRAC 2 cut(s) 690, 1051
HindII GTYRAC 2 cut(s) 690, 1051
HindIII AAGCTT 1 cut(s) 264
HinfI GANTC 5 cut(s) 163, 182, 905, 936, 1125
HphI GGTGA 1 cut(s) 914
Hpy166II GTNNAC 2 cut(s) 690, 1051
Hpy188I TCNGA 4 cut(s) 45, 187, 578, 867
Hpy188III TCNNGA 6 cut(s) 179, 407, 491, 679, 804, 1020
Hpy8I GTNNAC 2 cut(s) 690, 1051
Hpy99I CGWCG 2 cut(s) 10, 281
HpyAV CCTTC 2 cut(s) 63, 413
HpyCH4III ACNGT 2 cut(s) 158, 1048
HpyCH4IV ACGT 2 cut(s) 5, 514
HpyCH4V TGCA 9 cut(s) 83, 213, 755, 842, 945, 1001, 1016, 1074, 1119
HpyF10VI GCNNNNNNNGC 4 cut(s) 46, 317, 358, 1007
HpyF3I CTNAG 3 cut(s) 149, 312, 962
HpySE526I ACGT 2 cut(s) 5, 514
Hsp92I GRCGYC 3 cut(s) 5, 459, 501
Hsp92II CATG 3 cut(s) 468, 508, 682
HspAI GCGC 1 cut(s) 459
KasI GGCGCC 1 cut(s) 458
Kzo9I GATC 5 cut(s) 187, 273, 487, 772, 862
LmnI GCTCC 3 cut(s) 71, 734, 765
Lsp1109I GCAGC 4 cut(s) 67, 212, 398, 739
LweI GCATC 2 cut(s) 954, 1025
MabI ACCWGGT 1 cut(s) 344
MaeI CTAG 2 cut(s) 713, 1062
MaeII ACGT 2 cut(s) 5, 514
MalI GATC 5 cut(s) 189, 275, 489, 774, 864
MboI GATC 5 cut(s) 187, 273, 487, 772, 862
MboII GAAGA 3 cut(s) 869, 880, 1149
MfeI CAATTG 2 cut(s) 531, 584
MlsI TGGCCA 1 cut(s) 991
MluCI AATT 6 cut(s) 531, 584, 622, 756, 981, 1025
MluNI TGGCCA 1 cut(s) 991
Mly113I GGCGCC 1 cut(s) 459
MmeI TCCRAC 2 cut(s) 140, 637
MnlI CCTC 7 cut(s) 60, 335, 449, 620, 686, 711, 971
Mox20I TGGCCA 1 cut(s) 991
MroXI GAANNNNTTC 1 cut(s) 1141
MscI TGGCCA 1 cut(s) 991
MseI TTAA 5 cut(s) 414, 452, 548, 726, 759
Msp20I TGGCCA 1 cut(s) 991
MspR9I CCNGG 4 cut(s) 193, 346, 396, 848
MunI CAATTG 2 cut(s) 531, 584
MvaI CCWGG 4 cut(s) 193, 346, 396, 848
MwoI GCNNNNNNNGC 4 cut(s) 46, 317, 358, 1007
NarI GGCGCC 1 cut(s) 459
NcoI CCATGG 1 cut(s) 504
NdeI CATATG 1 cut(s) 209
NdeII GATC 5 cut(s) 187, 273, 487, 772, 862
NlaIII CATG 3 cut(s) 468, 508, 682
NlaIV GGNNCC 3 cut(s) 155, 343, 460
NspI RCATGY 1 cut(s) 468
PagI TCATGA 1 cut(s) 678
PdmI GAANNNNTTC 1 cut(s) 1141
PfeI GAWTC 5 cut(s) 163, 182, 905, 936, 1125
PfoI TCCNGGA 1 cut(s) 394
PkrI GCNGC 6 cut(s) 82, 202, 388, 463, 528, 754
PluTI GGCGCC 1 cut(s) 462
Psp6I CCWGG 4 cut(s) 191, 344, 394, 846
PspFI CCCAGC 1 cut(s) 113
PspGI CCWGG 4 cut(s) 191, 344, 394, 846
PspN4I GGNNCC 3 cut(s) 155, 343, 460
PspPI GGNCC 1 cut(s) 298
PstI CTGCAG 1 cut(s) 85
RsaI GTAC 4 cut(s) 469, 517, 786, 797
RsaNI GTAC 4 cut(s) 468, 516, 785, 796
SaqAI TTAA 5 cut(s) 414, 452, 548, 726, 759
SatI GCNGC 6 cut(s) 81, 201, 387, 462, 527, 753
Sau3AI GATC 5 cut(s) 187, 273, 487, 772, 862
Sau96I GGNCC 1 cut(s) 298
ScaI AGTACT 1 cut(s) 786
ScrFI CCNGG 4 cut(s) 193, 346, 396, 848
SexAI ACCWGGT 1 cut(s) 344
SfaNI GCATC 2 cut(s) 954, 1025
SfcI CTRYAG 2 cut(s) 81, 780
SfoI GGCGCC 1 cut(s) 460
SmlI CTYRAG 1 cut(s) 702
SmoI CTYRAG 1 cut(s) 702
Sse9I AATT 6 cut(s) 531, 584, 622, 756, 981, 1025
SsiI CCGC 4 cut(s) 301, 462, 526, 888
SspDI GGCGCC 1 cut(s) 458
SspMI CTAG 2 cut(s) 713, 1062
StyD4I CCNGG 4 cut(s) 191, 344, 394, 846
StyI CCWWGG 2 cut(s) 504, 712
TaaI ACNGT 2 cut(s) 158, 1048
TaiI ACGT 2 cut(s) 8, 517
TaqI TCGA 5 cut(s) 221, 276, 327, 490, 1091
TasI AATT 6 cut(s) 531, 584, 622, 756, 981, 1025
TatI WGTACW 2 cut(s) 784, 795
TauI GCSGC 2 cut(s) 464, 529
TfiI GAWTC 5 cut(s) 163, 182, 905, 936, 1125
Tru1I TTAA 5 cut(s) 414, 452, 548, 726, 759
Tru9I TTAA 5 cut(s) 414, 452, 548, 726, 759
TscAI CASTG 2 cut(s) 970, 1126
TseI GCWGC 4 cut(s) 80, 200, 386, 752
TspDTI ATGAA 6 cut(s) 124, 134, 207, 695, 1047, 1134
TspRI CASTG 2 cut(s) 970, 1126
XagI CCTNNNNNAGG 1 cut(s) 558
XceI RCATGY 1 cut(s) 468
XmaJI CCTAGG 1 cut(s) 712
XmnI GAANNNNTTC 1 cut(s) 1141
XspI CTAG 2 cut(s) 713, 1062
ZraI GACGTC 1 cut(s) 6
ZrmI AGTACT 1 cut(s) 786
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.