MD16G1001300.v1.1

Catalyzes the prenylation of para-hydroxybenzoate (PHB) with an all-trans polyprenyl group. Mediates the second step in the final reaction sequence of coenzyme Q (CoQ) biosynthesis, which is the condensation of the polyisoprenoid side chain with PHB, generating the first membrane-bound Q intermediate

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0001284
Physical Location & Seq
Reverse (-)
80820 .. 83538
2719 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1001300.v1.1.491

Sequence Viewer

Length: 711 bp
ATGTTTTGGCTGGCCTCTCGCGCTTCACGTAGGTTTCTGAAACCGTCAGTTTCCCTATTTGCAGTCTCTTCTTGTCACCTAACCACCGCCACCGCCAATCCTTACCCTAACTTTTATCAGAACGCAGGCCTGTTGGGCCCATTCAATCAAAGGCACCGTTGCACTCCCAGCTGTGTCGAATTTGATCTCAGACTCCTAGCGCACCTCTCGAGCTCGTCTTCGTCTACTGGCGCGAAGAACGACGGAAATCAGGGCTGCGATGGCGCGAAGGTCGATGCTTCTTGGATCGACTTGTATTTTCCGAGGCAAGCTCGGCCTTATGCTAAGCTCGCTCGCCTCGACCGACCTGGTTGCTCGCTTGGCCTTGCATGCATGTGCGTTGATCCAACTGTGGTGCTCCCATTGTACTTTTCCGGAGTATGCTGGACGCTTGTGTATGATACGATATATGCACATCAGGACAAGGAAGATGATATGAAAGTAGGTGTTAAGTCTACAGCATTGAGATTTGCAGATTCAACTGAGGAGGGATTACTGGGTGGGCCATATTATGCATTTTTGTCGGCTGCATCTAGACAATTAGCTTGGCAGATATGGACAGCTGACCTTTCATCCCGGGCTGATTGCAATAGAAAATTTGTGTTGAACAAATGGTTTGGTGCCATTATTTTCAGCGGAATCCCATTTAGAAGACTTTCATCGCAGGGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00130 ko01100 ko01110 map00130 map01100 map01110
Pfam Domains
Protein Families
UbiA

Protein Analysis

237

Amino Acids

26.14

Weight (kDa)

8.98

Isoelectric Point (pI)

47.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UbiA PF01040 129 - 182 2.9e-06 UbiA prenyltransferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 153, 659
AccI GTMKAC 2 cut(s) 224, 494
AccII CGCG 3 cut(s) 21, 233, 266
AccIII TCCGGA 1 cut(s) 413
AciI CCGC 3 cut(s) 87, 93, 675
AclWI GGATC 2 cut(s) 293, 377
AcsI RAATTY 2 cut(s) 179, 635
AfaI GTAC 1 cut(s) 407
AgsI TTSAA 3 cut(s) 145, 519, 646
AjnI CCWGG 1 cut(s) 346
AluBI AGCT 6 cut(s) 171, 213, 311, 328, 584, 602
AluI AGCT 6 cut(s) 171, 213, 311, 328, 584, 602
Alw21I GWGCWC 2 cut(s) 215, 399
Alw26I GTCTC 1 cut(s) 70
AlwI GGATC 2 cut(s) 293, 377
Ama87I CYCGRG 2 cut(s) 208, 615
Aor13HI TCCGGA 1 cut(s) 413
AoxI GGCC 6 cut(s) 12, 127, 136, 314, 361, 542
ApaI GGGCCC 1 cut(s) 140
ApeKI GCWGC 2 cut(s) 255, 566
ApoI RAATTY 2 cut(s) 179, 635
Asp700I GAANNNNTTC 1 cut(s) 694
AspLEI GCGC 4 cut(s) 23, 202, 233, 266
AspS9I GGNCC 3 cut(s) 136, 137, 542
AsuC2I CCSGG 2 cut(s) 616, 617
AsuHPI GGTGA 1 cut(s) 68
AvaI CYCGRG 2 cut(s) 208, 615
BaeGI GKGCMC 1 cut(s) 140
BanI GGYRCC 2 cut(s) 153, 659
BanII GRGCYC 2 cut(s) 140, 215
BbsI GAAGAC 2 cut(s) 210, 697
Bbv12I GWGCWC 2 cut(s) 215, 399
BbvI GCAGC 2 cut(s) 242, 553
BccI CCATC 1 cut(s) 254
BcgI CGANNNNNNTGC 4 cut(s) 333, 367, 543, 577
BciT130I CCWGG 1 cut(s) 348
BcnI CCSGG 2 cut(s) 616, 617
BcoDI GTCTC 1 cut(s) 70
BfaI CTAG 2 cut(s) 197, 573
BfmI CTRYAG 1 cut(s) 495
BglI GCCNNNNNGGC 1 cut(s) 135
BisI GCNGC 2 cut(s) 256, 567
BlpI GCTNAGC 1 cut(s) 324
BlsI GCNGC 2 cut(s) 257, 568
Bme1390I CCNGG 3 cut(s) 348, 616, 617
BmeT110I CYCGRG 2 cut(s) 208, 615
BmgT120I GGNCC 3 cut(s) 136, 137, 542
BmiI GGNNCC 3 cut(s) 138, 155, 661
BmrFI CCNGG 3 cut(s) 348, 616, 617
BmrI ACTGGG 1 cut(s) 545
BmsI GCATC 2 cut(s) 265, 578
BmuI ACTGGG 1 cut(s) 545
BpiI GAAGAC 2 cut(s) 210, 697
BplI GAGNNNNNCTC 2 cut(s) 295, 327
Bpu1102I GCTNAGC 1 cut(s) 324
BpuMI CCSGG 2 cut(s) 616, 617
BsaAI YACGTR 1 cut(s) 29
BsaJI CCNNGG 2 cut(s) 302, 615
BsaWI WCCGGW 1 cut(s) 413
Bse1I ACTGG 2 cut(s) 232, 540
BseAI TCCGGA 1 cut(s) 413
BseBI CCWGG 1 cut(s) 348
BseDI CCNNGG 2 cut(s) 302, 615
BseGI GGATG 1 cut(s) 611
BseMII CTCAG 2 cut(s) 202, 513
BseNI ACTGG 2 cut(s) 232, 540
BseRI GAGGAG 1 cut(s) 539
BseSI GKGCMC 1 cut(s) 140
BseXI GCAGC 2 cut(s) 242, 553
BseYI CCCAGC 1 cut(s) 167
Bsh1236I CGCG 3 cut(s) 21, 233, 266
Bsh1285I CGRYCG 1 cut(s) 343
BshFI GGCC 6 cut(s) 14, 129, 138, 316, 363, 544
BshNI GGYRCC 2 cut(s) 153, 659
BsiEI CGRYCG 1 cut(s) 343
BsiHKAI GWGCWC 2 cut(s) 215, 399
BsiHKCI CYCGRG 2 cut(s) 208, 615
BsiSI CCGG 2 cut(s) 414, 616
BsmAI GTCTC 1 cut(s) 70
BsnI GGCC 6 cut(s) 14, 129, 138, 316, 363, 544
BsoBI CYCGRG 2 cut(s) 208, 615
Bsp120I GGGCCC 1 cut(s) 136
Bsp1286I GDGCHC 3 cut(s) 140, 215, 399
Bsp13I TCCGGA 1 cut(s) 413
Bsp143I GATC 3 cut(s) 184, 285, 382
Bsp1720I GCTNAGC 1 cut(s) 324
BspACI CCGC 3 cut(s) 87, 93, 675
BspANI GGCC 6 cut(s) 14, 129, 138, 316, 363, 544
BspCNI CTCAG 2 cut(s) 201, 514
BspEI TCCGGA 1 cut(s) 413
BspFNI CGCG 3 cut(s) 21, 233, 266
BspLI GGNNCC 3 cut(s) 138, 155, 661
BspPI GGATC 2 cut(s) 293, 377
BspT107I GGYRCC 2 cut(s) 153, 659
BsrI ACTGG 2 cut(s) 232, 540
BssECI CCNNGG 2 cut(s) 302, 615
BssMI GATC 3 cut(s) 184, 285, 382
Bst2UI CCWGG 1 cut(s) 348
Bst4CI ACNGT 3 cut(s) 45, 158, 391
Bst6I CTCTTC 1 cut(s) 73
BstBAI YACGTR 1 cut(s) 29
BstC8I GCNNGC 7 cut(s) 12, 127, 309, 330, 334, 356, 370
BstDEI CTNAG 3 cut(s) 188, 324, 522
BstF5I GGATG 1 cut(s) 611
BstFNI CGCG 3 cut(s) 21, 233, 266
BstHHI GCGC 4 cut(s) 23, 202, 233, 266
BstKTI GATC 3 cut(s) 187, 288, 385
BstMAI GTCTC 1 cut(s) 70
BstMBI GATC 3 cut(s) 184, 285, 382
BstMCI CGRYCG 1 cut(s) 343
BstMWI GCNNNNNNNGC 8 cut(s) 20, 135, 168, 261, 313, 329, 360, 369
BstNI CCWGG 1 cut(s) 348
BstNSI RCATGY 2 cut(s) 372, 376
BstSCI CCNGG 3 cut(s) 346, 614, 615
BstSFI CTRYAG 1 cut(s) 495
BstSLI GKGCMC 1 cut(s) 140
BstUI CGCG 3 cut(s) 21, 233, 266
BstV1I GCAGC 2 cut(s) 242, 553
BstV2I GAAGAC 2 cut(s) 210, 697
BsuRI GGCC 6 cut(s) 14, 129, 138, 316, 363, 544
BtgZI GCGATG 2 cut(s) 273, 684
BtsCI GGATG 1 cut(s) 611
Cac8I GCNNGC 7 cut(s) 12, 127, 309, 330, 334, 356, 370
CfoI GCGC 4 cut(s) 23, 202, 233, 266
Cfr13I GGNCC 3 cut(s) 136, 137, 542
Cfr9I CCCGGG 1 cut(s) 615
CseI GACGC 1 cut(s) 436
CsiI ACCWGGT 1 cut(s) 346
Csp6I GTAC 1 cut(s) 406
CviAII CATG 2 cut(s) 369, 373
CviQI GTAC 1 cut(s) 406
DdeI CTNAG 3 cut(s) 188, 324, 522
DpnI GATC 3 cut(s) 186, 287, 384
DpnII GATC 3 cut(s) 184, 285, 382
Eam1104I CTCTTC 1 cut(s) 73
EarI CTCTTC 1 cut(s) 73
Ecl136II GAGCTC 1 cut(s) 213
Eco147I AGGCCT 1 cut(s) 129
Eco24I GRGCYC 2 cut(s) 140, 215
Eco53kI GAGCTC 1 cut(s) 213
Eco88I CYCGRG 2 cut(s) 208, 615
EcoICRI GAGCTC 1 cut(s) 213
EcoRII CCWGG 1 cut(s) 346
EcoT22I ATGCAT 2 cut(s) 374, 556
EcoT38I GRGCYC 2 cut(s) 140, 215
FaeI CATG 2 cut(s) 372, 376
FatI CATG 2 cut(s) 368, 372
FblI GTMKAC 2 cut(s) 224, 494
Fnu4HI GCNGC 2 cut(s) 256, 567
FokI GGATG 1 cut(s) 598
FriOI GRGCYC 2 cut(s) 140, 215
Fsp4HI GCNGC 2 cut(s) 256, 567
FspBI CTAG 2 cut(s) 197, 573
GlaI GCGC 4 cut(s) 22, 201, 232, 265
GluI GCNGC 2 cut(s) 256, 567
GsaI CCCAGC 1 cut(s) 171
HaeIII GGCC 6 cut(s) 14, 129, 138, 316, 363, 544
HapII CCGG 2 cut(s) 414, 616
HgaI GACGC 1 cut(s) 436
HhaI GCGC 4 cut(s) 23, 202, 233, 266
Hin1II CATG 2 cut(s) 372, 376
Hin6I GCGC 4 cut(s) 21, 200, 231, 264
HinP1I GCGC 4 cut(s) 21, 200, 231, 264
HinfI GANTC 3 cut(s) 192, 515, 678
HpaII CCGG 2 cut(s) 414, 616
HphI GGTGA 1 cut(s) 68
Hpy166II GTNNAC 2 cut(s) 225, 495
Hpy188I TCNGA 4 cut(s) 39, 120, 191, 303
Hpy188III TCNNGA 4 cut(s) 208, 414, 458, 573
Hpy8I GTNNAC 2 cut(s) 225, 495
Hpy99I CGWCG 1 cut(s) 245
HpyAV CCTTC 1 cut(s) 262
HpyCH4III ACNGT 3 cut(s) 45, 158, 391
HpyCH4IV ACGT 1 cut(s) 28
HpyCH4V TGCA 9 cut(s) 62, 162, 368, 372, 452, 512, 554, 569, 627
HpyF10VI GCNNNNNNNGC 8 cut(s) 20, 135, 168, 261, 313, 329, 360, 369
HpyF3I CTNAG 3 cut(s) 188, 324, 522
HpySE526I ACGT 1 cut(s) 28
Hsp92II CATG 2 cut(s) 372, 376
HspAI GCGC 4 cut(s) 21, 200, 231, 264
Kpn2I TCCGGA 1 cut(s) 413
Kzo9I GATC 3 cut(s) 184, 285, 382
LmnI GCTCC 1 cut(s) 402
Lsp1109I GCAGC 2 cut(s) 242, 553
LweI GCATC 2 cut(s) 265, 578
MabI ACCWGGT 1 cut(s) 346
MaeI CTAG 2 cut(s) 197, 573
MaeII ACGT 1 cut(s) 28
MaeIII GTNAC 1 cut(s) 74
MalI GATC 3 cut(s) 186, 287, 384
MboI GATC 3 cut(s) 184, 285, 382
MboII GAAGA 5 cut(s) 60, 210, 247, 479, 702
MhlI GDGCHC 3 cut(s) 140, 215, 399
MluCI AATT 3 cut(s) 179, 578, 635
MlyI GAGTC 1 cut(s) 186
MmeI TCCRAC 1 cut(s) 410
MnlI CCTC 6 cut(s) 25, 215, 297, 347, 517, 520
Mph1103I ATGCAT 2 cut(s) 374, 556
MroI TCCGGA 1 cut(s) 413
MroXI GAANNNNTTC 1 cut(s) 694
MseI TTAA 1 cut(s) 489
MslI CAYNNNNRTG 1 cut(s) 373
MspA1I CMGCKG 3 cut(s) 171, 602, 675
MspI CCGG 2 cut(s) 414, 616
MspR9I CCNGG 3 cut(s) 348, 616, 617
MvaI CCWGG 1 cut(s) 348
MvnI CGCG 3 cut(s) 21, 233, 266
MwoI GCNNNNNNNGC 8 cut(s) 20, 135, 168, 261, 313, 329, 360, 369
NciI CCSGG 2 cut(s) 616, 617
NdeII GATC 3 cut(s) 184, 285, 382
NlaIII CATG 2 cut(s) 372, 376
NlaIV GGNNCC 3 cut(s) 138, 155, 661
NmeAIII GCCGAG 1 cut(s) 292
NmuCI GTSAC 1 cut(s) 74
NsiI ATGCAT 2 cut(s) 374, 556
NspI RCATGY 2 cut(s) 372, 376
PaeI GCATGC 1 cut(s) 372
PaeR7I CTCGAG 1 cut(s) 208
PceI AGGCCT 1 cut(s) 129
PcsI WCGNNNNNNNCGW 3 cut(s) 25, 336, 340
PdmI GAANNNNTTC 1 cut(s) 694
PfeI GAWTC 2 cut(s) 515, 678
PkrI GCNGC 2 cut(s) 257, 568
PleI GAGTC 1 cut(s) 186
PpsI GAGTC 1 cut(s) 186
Ppu21I YACGTR 1 cut(s) 29
Psp124BI GAGCTC 1 cut(s) 215
Psp6I CCWGG 1 cut(s) 346
PspFI CCCAGC 1 cut(s) 167
PspGI CCWGG 1 cut(s) 346
PspN4I GGNNCC 3 cut(s) 138, 155, 661
PspOMI GGGCCC 1 cut(s) 136
PspPI GGNCC 3 cut(s) 136, 137, 542
PvuII CAGCTG 2 cut(s) 171, 602
RsaI GTAC 1 cut(s) 407
RsaNI GTAC 1 cut(s) 406
RseI CAYNNNNRTG 1 cut(s) 373
SacI GAGCTC 1 cut(s) 215
SaqAI TTAA 1 cut(s) 489
SatI GCNGC 2 cut(s) 256, 567
Sau3AI GATC 3 cut(s) 184, 285, 382
Sau96I GGNCC 3 cut(s) 136, 137, 542
SchI GAGTC 1 cut(s) 186
ScrFI CCNGG 3 cut(s) 348, 616, 617
SduI GDGCHC 3 cut(s) 140, 215, 399
SexAI ACCWGGT 1 cut(s) 346
SfaNI GCATC 2 cut(s) 265, 578
SfcI CTRYAG 1 cut(s) 495
SfiI GGCCNNNNNGGCC 1 cut(s) 135
Sfr274I CTCGAG 1 cut(s) 208
SlaI CTCGAG 1 cut(s) 208
SmaI CCCGGG 1 cut(s) 617
SmiMI CAYNNNNRTG 1 cut(s) 373
SmlI CTYRAG 1 cut(s) 208
SmoI CTYRAG 1 cut(s) 208
SphI GCATGC 1 cut(s) 372
Sse9I AATT 3 cut(s) 179, 578, 635
SseBI AGGCCT 1 cut(s) 129
SsiI CCGC 3 cut(s) 87, 93, 675
SspMI CTAG 2 cut(s) 197, 573
SstI GAGCTC 1 cut(s) 215
StuI AGGCCT 1 cut(s) 129
StyD4I CCNGG 3 cut(s) 346, 614, 615
TaaI ACNGT 3 cut(s) 45, 158, 391
TaiI ACGT 1 cut(s) 31
TaqI TCGA 5 cut(s) 177, 209, 273, 288, 339
TaqII GACCGA 1 cut(s) 357
TasI AATT 3 cut(s) 179, 578, 635
TatI WGTACW 1 cut(s) 405
TfiI GAWTC 2 cut(s) 515, 678
Tru1I TTAA 1 cut(s) 489
Tru9I TTAA 1 cut(s) 489
TseFI GTSAC 1 cut(s) 74
TseI GCWGC 2 cut(s) 255, 566
Tsp45I GTSAC 1 cut(s) 74
TspDTI ATGAA 3 cut(s) 491, 600, 687
TspGWI ACGGA 1 cut(s) 258
TspMI CCCGGG 1 cut(s) 615
XapI RAATTY 2 cut(s) 179, 635
XbaI TCTAGA 1 cut(s) 572
XceI RCATGY 2 cut(s) 372, 376
XhoI CTCGAG 1 cut(s) 208
XmaI CCCGGG 1 cut(s) 615
XmiI GTMKAC 2 cut(s) 224, 494
XmnI GAANNNNTTC 1 cut(s) 694
XspI CTAG 2 cut(s) 197, 573
Zsp2I ATGCAT 2 cut(s) 374, 556
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.