Rorug05G0219700

Catalyzes the prenylation of para-hydroxybenzoate (PHB) with an all-trans polyprenyl group. Mediates the second step in the final reaction sequence of coenzyme Q (CoQ) biosynthesis, which is the condensation of the polyisoprenoid side chain with PHB, generating the first membrane-bound Q intermediate

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0001284
Physical Location & Seq
Reverse (-)
22639858 .. 22641690
1833 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0219700.1

Sequence Viewer

Length: 1833 bp
ATGGAGTTGCCGGTGAAGATTCCGGTCCACGGTGGCCAGAGAACACCATATAGAATTGAAACCAGAAATCTGAGCTACAAATTATGTGCCCAATTTGATGAAATGAAATGGGTGCTTTGTGGTGGTGCTAGTTCCAAGAGAGTTCCAAGCTTCATATTGAAGAATGTCAACTGTGAAGCAAGGCCTGGTGAGATCACTGCCATTGCTGGTCCTAGTGGGGCTGGGAAAACCACACTGCTAGAGATTCTTGCTGGAAAGATCTCCCCAAAGAAAGTGTGTGGTCAAGTTCTGGTCAATAATCTTCCCATGGAGGCTAAAAGTTTTCGGAGAATGTCGGGGTATGTTACTCAAGATGATGCTCTATTCCCATTACTTACGGTTGAAGAAACACTCATGTATAGTGCCCTTCTGAGGCATCCTGGTGGGAGAAAGGACGCTGCAGCCAAAGTGAGGCAGCTGATGAAAGAGCTTGGGCTGGAACATGTTGCGGCTTCAAGAATAGGTGGGGGATCAAGCAATAATGGGATTTCAGGTGGAGAAAGGCGCAGAGTTTCAGTTGGAGTTGATTTAGTGCATGATCCAGCTGTGATTTTGATTGATGAACCAACTTCAGGTCTGGATTCTGCCTCAGCTTTCCATATAGTGTCATTGCTCAAAACAATGGTTGTCAATCAAGGCAAGACTATTGTTCTCACCATTCACCAACCAGGTTTTCGGATCTTAGAAATGTTTGATCGCCTTGTTCTGCTTTCCAATGGAACTGTCATGCACAACGGATCGATGCATTTTCTCAAAGAAAGGCTCAAGTTTGCAGGCCATTGCATCCCCAACCATGTCAACATGCTTGAATTTGCAATCGATGTTATTGAAAGCTTGGATACACAAATTCCAGAAGCCTTAAACAACCGACGTTTCCTCAGAGGGAATCATAAGTTTGAGCACCATTTTCACAGTTTTGAAGAAAAGAAGCAACTCTACCGGAATTCTAGACTCGAGGAAGTTGTTATACTAGGAGAAAGATTCTGCAGCAACATTTTCAGAACCAAGCAACTGTTTGTCACAAGAGTCGTACAAGCCTTGATAGCTGGGTTACTACTCGGAACCATATTTCTAAACGTAGGCAGACAACAAAGTACAATCGCTTTACAAACCCAAACTGGTTTTTTTGCCTTCAGCCTCACTTTCTTGCTATCTTCCACAACAGAAGGCCTGCCGATTTTCTTACAAGAGAGAAGAATACTAATGAGTGAGACTTCCAAAGGAGCCTACAGAGTTTCCTCCTATGTTATAGCAAACACCCTCATATTTCTTCCTTTCCTTTTGATGGTTGGTATTCTCTTTTCCACTCCGGTTTACTGGTTGATTGGATTGAAGGGAGACGTCGACAAGTTCATCTACTTCAATCTTGTGGTCTGGATGGTTCTTCTGATGTCCAATTCTGTTGTAGCATGTTTCAGTGCTCTTGTGCCTAACTTCATCATGGGGACATCAGTTGTTTCGGGGCTAATGGGATCGTTCTTTCTCTTTTCCGGATACTTTATTCCAAAGGAGAACATTCCAAGTTACTGGCTTTTCATGCATTACTCAAGTCTTTTCAAGTACCCTTTTGAGTGCTTTATGATAAATGAGTATGGAGGAGAGCAGGGAAGAAGGTGCATTCAGATTATTGATGAGGGAGTATGCAATCTATATGGGGATGAATTGTTAAGACAGCAGAATTTAAGAGACACACAAAAATGGAGCAATTTGGCTGTGATGCTGGGTTTCATAGTTGGATATAGAGTCCTTTGTTTTCTTATTCTGTGGTACAGATGCTACAGAAACATAAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00130 ko01100 ko01110 map00130 map01100 map01110
Pfam Domains
Protein Families
UbiA

Protein Analysis

610

Amino Acids

68.67

Weight (kDa)

9.17

Isoelectric Point (pI)

41.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC_tran PF00005 53 - 204 1.7e-29 ABC transporter
ABC2_membrane PF01061 334 - 544 1e-48 ABC-2 type transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1383
AccI GTMKAC 1 cut(s) 1383
AccIII TCCGGA 1 cut(s) 1529
AciI CCGC 1 cut(s) 488
AclWI GGATC 5 cut(s) 517, 572, 725, 784, 1519
AcoI YGGCCR 1 cut(s) 34
AcsI RAATTY 4 cut(s) 848, 885, 982, 1717
AcuI CTGAAG 2 cut(s) 594, 1156
AcyI GRCGYC 1 cut(s) 1380
AfaI GTAC 4 cut(s) 1071, 1135, 1601, 1808
AfiI CCNNNNNNNGG 5 cut(s) 29, 411, 450, 611, 1324
AflIII ACRYGT 1 cut(s) 481
AjnI CCWGG 3 cut(s) 184, 418, 706
AjuI GAANNNNNNNTTGG 2 cut(s) 696, 728
AluBI AGCT 8 cut(s) 75, 150, 457, 469, 584, 632, 873, 1085
AluI AGCT 8 cut(s) 75, 150, 457, 469, 584, 632, 873, 1085
Alw21I GWGCWC 2 cut(s) 942, 1462
Alw26I GTCTC 3 cut(s) 1244, 1371, 1719
AlwI GGATC 5 cut(s) 517, 572, 725, 784, 1519
Ama87I CYCGRG 1 cut(s) 992
Aor13HI TCCGGA 1 cut(s) 1529
AoxI GGCC 4 cut(s) 34, 182, 814, 1207
ApeKI GCWGC 4 cut(s) 437, 440, 454, 1026
ApoI RAATTY 4 cut(s) 848, 885, 982, 1717
AspLEI GCGC 1 cut(s) 546
AspS9I GGNCC 2 cut(s) 25, 209
AsuHPI GGTGA 4 cut(s) 25, 200, 685, 692
AvaI CYCGRG 1 cut(s) 992
AvaII GGWCC 2 cut(s) 25, 209
BaeGI GKGCMC 2 cut(s) 91, 406
BalI TGGCCA 1 cut(s) 36
BarI GAAGNNNNNNTAC 4 cut(s) 959, 990, 991, 1022
Bbv12I GWGCWC 2 cut(s) 942, 1462
BbvCI CCTCAGC 1 cut(s) 628
BbvI GCAGC 4 cut(s) 424, 452, 466, 1038
BccI CCATC 2 cut(s) 1318, 1411
BciT130I CCWGG 3 cut(s) 186, 420, 708
BciVI GTATCC 2 cut(s) 871, 1526
BcoDI GTCTC 3 cut(s) 1244, 1371, 1719
BfaI CTAG 6 cut(s) 129, 213, 239, 987, 1010, 1831
BfmI CTRYAG 4 cut(s) 438, 1024, 1267, 1816
BfuI GTATCC 2 cut(s) 871, 1526
BglII AGATCT 1 cut(s) 258
BisI GCNGC 5 cut(s) 438, 441, 455, 489, 1027
BlsI GCNGC 5 cut(s) 439, 442, 456, 490, 1028
Bme1390I CCNGG 3 cut(s) 186, 420, 708
Bme18I GGWCC 2 cut(s) 25, 209
BmeT110I CYCGRG 1 cut(s) 992
BmgT120I GGNCC 2 cut(s) 25, 209
BmiI GGNNCC 2 cut(s) 1102, 1264
BmrFI CCNGG 3 cut(s) 186, 420, 708
BmsI GCATC 6 cut(s) 346, 424, 771, 831, 1746, 1802
Bpu10I CCTNAGC 1 cut(s) 628
BpuEI CTTGAG 3 cut(s) 333, 788, 1570
Bsa29I ATCGAT 2 cut(s) 779, 858
BsaHI GRCGYC 1 cut(s) 1380
BsaJI CCNNGG 2 cut(s) 28, 306
BsaWI WCCGGW 4 cut(s) 22, 978, 1348, 1529
Bsc4I CCNNNNNNNGG 5 cut(s) 29, 411, 450, 611, 1324
Bse118I RCCGGY 1 cut(s) 10
Bse1I ACTGG 3 cut(s) 1162, 1361, 1571
Bse3DI GCAATG 3 cut(s) 201, 647, 817
BseAI TCCGGA 1 cut(s) 1529
BseBI CCWGG 3 cut(s) 186, 420, 708
BseCI ATCGAT 2 cut(s) 779, 858
BseDI CCNNGG 2 cut(s) 28, 306
BseGI GGATG 4 cut(s) 415, 822, 1422, 1702
BseLI CCNNNNNNNGG 5 cut(s) 29, 411, 450, 611, 1324
BseMI GCAATG 3 cut(s) 201, 647, 817
BseMII CTCAG 4 cut(s) 62, 401, 642, 931
BseNI ACTGG 3 cut(s) 1162, 1361, 1571
BseRI GAGGAG 1 cut(s) 1650
BseSI GKGCMC 2 cut(s) 91, 406
BseXI GCAGC 4 cut(s) 424, 452, 466, 1038
BseYI CCCAGC 3 cut(s) 221, 1085, 1759
BshFI GGCC 4 cut(s) 36, 184, 816, 1209
BshVI ATCGAT 2 cut(s) 779, 858
BsiHKAI GWGCWC 2 cut(s) 942, 1462
BsiHKCI CYCGRG 1 cut(s) 992
BsiSI CCGG 5 cut(s) 11, 23, 979, 1349, 1530
BslFI GGGAC 1 cut(s) 1498
BslI CCNNNNNNNGG 5 cut(s) 29, 411, 450, 611, 1324
BsmAI GTCTC 3 cut(s) 1244, 1371, 1719
BsmBI CGTCTC 1 cut(s) 1371
BsmFI GGGAC 1 cut(s) 1498
BsmI GAATGC 1 cut(s) 1656
BsnI GGCC 4 cut(s) 36, 184, 816, 1209
BsoBI CYCGRG 1 cut(s) 992
Bsp1286I GDGCHC 4 cut(s) 91, 406, 942, 1462
Bsp13I TCCGGA 1 cut(s) 1529
Bsp143I GATC 8 cut(s) 192, 258, 509, 577, 717, 733, 776, 1511
Bsp19I CCATGG 1 cut(s) 306
BspACI CCGC 1 cut(s) 488
BspANI GGCC 4 cut(s) 36, 184, 816, 1209
BspCNI CTCAG 4 cut(s) 63, 402, 641, 930
BspDI ATCGAT 2 cut(s) 779, 858
BspEI TCCGGA 1 cut(s) 1529
BspLI GGNNCC 2 cut(s) 1102, 1264
BspMAI CTGCAG 2 cut(s) 442, 1028
BspPI GGATC 5 cut(s) 517, 572, 725, 784, 1519
BsrDI GCAATG 3 cut(s) 201, 647, 817
BsrFI RCCGGY 1 cut(s) 10
BsrI ACTGG 3 cut(s) 1162, 1361, 1571
BssAI RCCGGY 1 cut(s) 10
BssECI CCNNGG 2 cut(s) 28, 306
BssMI GATC 8 cut(s) 192, 258, 509, 577, 717, 733, 776, 1511
BssNI GRCGYC 1 cut(s) 1380
BssT1I CCWWGG 1 cut(s) 306
Bst2UI CCWGG 3 cut(s) 186, 420, 708
Bst4CI ACNGT 6 cut(s) 32, 173, 379, 763, 953, 1053
BstACI GRCGYC 1 cut(s) 1380
BstC8I GCNNGC 2 cut(s) 814, 1211
BstDEI CTNAG 5 cut(s) 71, 410, 628, 721, 917
BstDSI CCRYGG 2 cut(s) 28, 306
BstF5I GGATG 4 cut(s) 415, 822, 1422, 1702
BstHHI GCGC 1 cut(s) 546
BstKTI GATC 8 cut(s) 195, 261, 512, 580, 720, 736, 779, 1514
BstMAI GTCTC 3 cut(s) 1244, 1371, 1719
BstMBI GATC 8 cut(s) 192, 258, 509, 577, 717, 733, 776, 1511
BstMWI GCNNNNNNNGC 2 cut(s) 1082, 1576
BstNI CCWGG 3 cut(s) 186, 420, 708
BstNSI RCATGY 3 cut(s) 485, 844, 1452
BstSCI CCNGG 3 cut(s) 184, 418, 706
BstSFI CTRYAG 4 cut(s) 438, 1024, 1267, 1816
BstSLI GKGCMC 2 cut(s) 91, 406
BstV1I GCAGC 4 cut(s) 424, 452, 466, 1038
BstX2I RGATCY 2 cut(s) 258, 717
BstXI CCANNNNNNTGG 1 cut(s) 1566
BstYI RGATCY 2 cut(s) 258, 717
Bsu15I ATCGAT 2 cut(s) 779, 858
BsuI GTATCC 2 cut(s) 871, 1526
BsuRI GGCC 4 cut(s) 36, 184, 816, 1209
BsuTUI ATCGAT 2 cut(s) 779, 858
BtgI CCRYGG 2 cut(s) 28, 306
BtsCI GGATG 4 cut(s) 415, 822, 1422, 1702
BtsI GCAGTG 2 cut(s) 195, 233
BtsIMutI CAGTG 3 cut(s) 195, 233, 1462
Cac8I GCNNGC 2 cut(s) 814, 1211
CfoI GCGC 1 cut(s) 546
Cfr10I RCCGGY 1 cut(s) 10
Cfr13I GGNCC 2 cut(s) 25, 209
ClaI ATCGAT 2 cut(s) 779, 858
CseI GACGC 1 cut(s) 443
CsiI ACCWGGT 1 cut(s) 706
Csp6I GTAC 4 cut(s) 1070, 1134, 1600, 1807
CviQI GTAC 4 cut(s) 1070, 1134, 1600, 1807
DdeI CTNAG 5 cut(s) 71, 410, 628, 721, 917
DpnI GATC 8 cut(s) 194, 260, 511, 579, 719, 735, 778, 1513
DpnII GATC 8 cut(s) 192, 258, 509, 577, 717, 733, 776, 1511
EaeI YGGCCR 1 cut(s) 34
Eco130I CCWWGG 1 cut(s) 306
Eco147I AGGCCT 2 cut(s) 184, 1209
Eco47I GGWCC 2 cut(s) 25, 209
Eco57I CTGAAG 2 cut(s) 594, 1156
Eco88I CYCGRG 1 cut(s) 992
EcoRI GAATTC 1 cut(s) 982
EcoRII CCWGG 3 cut(s) 184, 418, 706
EcoT14I CCWWGG 1 cut(s) 306
EcoT22I ATGCAT 2 cut(s) 786, 1581
ErhI CCWWGG 1 cut(s) 306
Esp3I CGTCTC 1 cut(s) 1371
FaqI GGGAC 1 cut(s) 1498
FblI GTMKAC 1 cut(s) 1383
Fnu4HI GCNGC 5 cut(s) 438, 441, 455, 489, 1027
FokI GGATG 4 cut(s) 402, 809, 1429, 1709
Fsp4HI GCNGC 5 cut(s) 438, 441, 455, 489, 1027
FspBI CTAG 6 cut(s) 129, 213, 239, 987, 1010, 1831
GlaI GCGC 1 cut(s) 545
GluI GCNGC 5 cut(s) 438, 441, 455, 489, 1027
GsaI CCCAGC 3 cut(s) 225, 1089, 1763
HaeIII GGCC 4 cut(s) 36, 184, 816, 1209
HapII CCGG 5 cut(s) 11, 23, 979, 1349, 1530
HgaI GACGC 1 cut(s) 443
HhaI GCGC 1 cut(s) 546
Hin1I GRCGYC 1 cut(s) 1380
Hin6I GCGC 1 cut(s) 544
HinP1I GCGC 1 cut(s) 544
HincII GTYRAC 3 cut(s) 169, 838, 1384
HindII GTYRAC 3 cut(s) 169, 838, 1384
HindIII AAGCTT 2 cut(s) 148, 871
HinfI GANTC 8 cut(s) 19, 244, 620, 925, 990, 1020, 1065, 1782
HpaII CCGG 5 cut(s) 11, 23, 979, 1349, 1530
HphI GGTGA 4 cut(s) 25, 200, 685, 692
Hpy166II GTNNAC 5 cut(s) 28, 169, 838, 1354, 1384
Hpy188I TCNGA 9 cut(s) 72, 327, 411, 717, 920, 1040, 1100, 1428, 1662
Hpy188III TCNNGA 7 cut(s) 350, 495, 617, 890, 987, 1414, 1530
Hpy8I GTNNAC 5 cut(s) 28, 169, 838, 1354, 1384
Hpy99I CGWCG 2 cut(s) 912, 1385
HpyAV CCTTC 5 cut(s) 416, 1180, 1199, 1366, 1644
HpyCH4III ACNGT 6 cut(s) 32, 173, 379, 763, 953, 1053
HpyCH4IV ACGT 3 cut(s) 910, 1116, 1380
HpyF10VI GCNNNNNNNGC 2 cut(s) 1082, 1576
HpyF3I CTNAG 5 cut(s) 71, 410, 628, 721, 917
HpySE526I ACGT 3 cut(s) 910, 1116, 1380
Hsp92I GRCGYC 1 cut(s) 1380
HspAI GCGC 1 cut(s) 544
Kpn2I TCCGGA 1 cut(s) 1529
Kzo9I GATC 8 cut(s) 192, 258, 509, 577, 717, 733, 776, 1511
LmnI GCTCC 2 cut(s) 1262, 1740
Lsp1109I GCAGC 4 cut(s) 424, 452, 466, 1038
LweI GCATC 6 cut(s) 346, 424, 771, 831, 1746, 1802
MabI ACCWGGT 1 cut(s) 706
MaeI CTAG 6 cut(s) 129, 213, 239, 987, 1010, 1831
MaeII ACGT 3 cut(s) 910, 1116, 1380
MaeIII GTNAC 4 cut(s) 343, 1057, 1089, 1562
MalI GATC 8 cut(s) 194, 260, 511, 579, 719, 735, 778, 1513
MboI GATC 8 cut(s) 192, 258, 509, 577, 717, 733, 776, 1511
MflI RGATCY 2 cut(s) 258, 717
MhlI GDGCHC 4 cut(s) 91, 406, 942, 1462
MlsI TGGCCA 1 cut(s) 36
MluNI TGGCCA 1 cut(s) 36
MlyI GAGTC 3 cut(s) 984, 1074, 1791
MmeI TCCRAC 2 cut(s) 538, 1753
Mox20I TGGCCA 1 cut(s) 36
Mph1103I ATGCAT 2 cut(s) 786, 1581
MroI TCCGGA 1 cut(s) 1529
MscI TGGCCA 1 cut(s) 36
MseI TTAA 3 cut(s) 899, 1706, 1721
MslI CAYNNNNRTG 2 cut(s) 420, 1735
Msp20I TGGCCA 1 cut(s) 36
MspA1I CMGCKG 2 cut(s) 457, 584
MspI CCGG 5 cut(s) 11, 23, 979, 1349, 1530
MspR9I CCNGG 3 cut(s) 186, 420, 708
Mva1269I GAATGC 1 cut(s) 1656
MvaI CCWGG 3 cut(s) 186, 420, 708
MwoI GCNNNNNNNGC 2 cut(s) 1082, 1576
NcoI CCATGG 1 cut(s) 306
NdeII GATC 8 cut(s) 192, 258, 509, 577, 717, 733, 776, 1511
NlaIV GGNNCC 2 cut(s) 1102, 1264
NmuCI GTSAC 1 cut(s) 1057
NsiI ATGCAT 2 cut(s) 786, 1581
NspI RCATGY 3 cut(s) 485, 844, 1452
PaeR7I CTCGAG 1 cut(s) 992
PceI AGGCCT 2 cut(s) 184, 1209
PciI ACATGT 1 cut(s) 481
PctI GAATGC 1 cut(s) 1656
PfeI GAWTC 5 cut(s) 19, 244, 620, 925, 1020
PkrI GCNGC 5 cut(s) 439, 442, 456, 490, 1028
PleI GAGTC 3 cut(s) 984, 1073, 1790
PpsI GAGTC 3 cut(s) 984, 1073, 1790
PscI ACATGT 1 cut(s) 481
Psp6I CCWGG 3 cut(s) 184, 418, 706
PspFI CCCAGC 3 cut(s) 221, 1085, 1759
PspGI CCWGG 3 cut(s) 184, 418, 706
PspN4I GGNNCC 2 cut(s) 1102, 1264
PspPI GGNCC 2 cut(s) 25, 209
PspXI VCTCGAGB 1 cut(s) 992
PstI CTGCAG 2 cut(s) 442, 1028
PsuI RGATCY 2 cut(s) 258, 717
PvuII CAGCTG 2 cut(s) 457, 584
RsaI GTAC 4 cut(s) 1071, 1135, 1601, 1808
RsaNI GTAC 4 cut(s) 1070, 1134, 1600, 1807
RseI CAYNNNNRTG 2 cut(s) 420, 1735
SalI GTCGAC 1 cut(s) 1382
SaqAI TTAA 3 cut(s) 899, 1706, 1721
SatI GCNGC 5 cut(s) 438, 441, 455, 489, 1027
Sau3AI GATC 8 cut(s) 192, 258, 509, 577, 717, 733, 776, 1511
Sau96I GGNCC 2 cut(s) 25, 209
SchI GAGTC 3 cut(s) 984, 1074, 1791
ScrFI CCNGG 3 cut(s) 186, 420, 708
SduI GDGCHC 4 cut(s) 91, 406, 942, 1462
SexAI ACCWGGT 1 cut(s) 706
SfaNI GCATC 6 cut(s) 346, 424, 771, 831, 1746, 1802
SfcI CTRYAG 4 cut(s) 438, 1024, 1267, 1816
Sfr274I CTCGAG 1 cut(s) 992
SinI GGWCC 2 cut(s) 25, 209
SlaI CTCGAG 1 cut(s) 992
SmiMI CAYNNNNRTG 2 cut(s) 420, 1735
SmlI CTYRAG 4 cut(s) 348, 803, 992, 1585
SmoI CTYRAG 4 cut(s) 348, 803, 992, 1585
SseBI AGGCCT 2 cut(s) 184, 1209
SsiI CCGC 1 cut(s) 488
SspMI CTAG 6 cut(s) 129, 213, 239, 987, 1010, 1831
StuI AGGCCT 2 cut(s) 184, 1209
StyD4I CCNGG 3 cut(s) 184, 418, 706
StyI CCWWGG 1 cut(s) 306
TaaI ACNGT 6 cut(s) 32, 173, 379, 763, 953, 1053
TaiI ACGT 3 cut(s) 913, 1119, 1383
TaqI TCGA 4 cut(s) 779, 858, 993, 1383
TatI WGTACW 1 cut(s) 1133
TauI GCSGC 1 cut(s) 491
TfiI GAWTC 5 cut(s) 19, 244, 620, 925, 1020
Tru1I TTAA 3 cut(s) 899, 1706, 1721
Tru9I TTAA 3 cut(s) 899, 1706, 1721
TscAI CASTG 3 cut(s) 202, 240, 1462
TseFI GTSAC 1 cut(s) 1057
TseI GCWGC 4 cut(s) 437, 440, 454, 1026
Tsp45I GTSAC 1 cut(s) 1057
TspGWI ACGGA 1 cut(s) 789
TspRI CASTG 3 cut(s) 202, 240, 1462
VpaK11BI GGWCC 2 cut(s) 25, 209
XapI RAATTY 4 cut(s) 848, 885, 982, 1717
XbaI TCTAGA 1 cut(s) 986
XceI RCATGY 3 cut(s) 485, 844, 1452
XhoI CTCGAG 1 cut(s) 992
XmiI GTMKAC 1 cut(s) 1383
XspI CTAG 6 cut(s) 129, 213, 239, 987, 1010, 1831
ZraI GACGTC 1 cut(s) 1381
Zsp2I ATGCAT 2 cut(s) 786, 1581
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.