RchiOBHm_Chr5g0044921

Catalyzes the prenylation of para-hydroxybenzoate (PHB) with an all-trans polyprenyl group. Mediates the second step in the final reaction sequence of coenzyme Q (CoQ) biosynthesis, which is the condensation of the polyisoprenoid side chain with PHB, generating the first membrane-bound Q intermediate

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0001284
Physical Location & Seq
Reverse (-)
40416258 .. 40418932
2675 bp
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UTR
Exon/CDS
Intron
PRQ32306

Sequence Viewer

Length: 648 bp
ATGGTGGAACGTACAAAGTTGCGGCCAATTGCGAGTGGTCTTTTAACACCTTTTCAAGGGTTGTGTTTTCTCGGATTTCAATTGCTTTTTGTTCTTGGGACTCTCCTCCAACTGAATAATTATAGCCGTGTTTTGGGGGCTTCGTCTTTGTTGCTATTTTTCTCCTATCCCCTCATGAAAAGGTTGACATTCTGGCCTCAAGCATACCTAGGTTTGACCTTTAACTGGGGAGCTTTGTTAGGATGGGCTGCAATTAAAGGGAGCATTGATCCAGCTATAGTACTTCCATTGTACATTTCTGGAGTATTTTGGACACTTGTGTATGATACGATATATGCACATCAGGATAAAGAAGATGATCTGAAGATTGGTGTCAAATCTACCGCTTTGAAGTTTGGTGATTCAACAAAGGAGTGGATTACTGGGTTTGGAATCCTGTGCATCAGTAGTCTTGCCCTCAGTGGATATATTGCCGAAATTGGCTGGCCATATTATGCACTTCTGGCAGTTGCATCTGGACAATTAGCTTGGCAGATATGGACGGTTGACCTTTCATCTAGGCTTGATTGCAATAGAAAGTTTGTGTCGAACAAGTGGTTTGGTGCTATTATTTGCAGTGGAATCTTATTGGGAAGACTTTCATCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00130 ko01100 ko01110 map00130 map01100 map01110
Pfam Domains
Protein Families
UbiA

Protein Analysis

215

Amino Acids

23.88

Weight (kDa)

9.05

Isoelectric Point (pI)

24.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UbiA PF01040 2 - 196 2.9e-43 UbiA prenyltransferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 22, 384
AclWI GGATC 1 cut(s) 263
AcoI YGGCCR 2 cut(s) 23, 485
AcuI CTGAAG 1 cut(s) 383
AfaI GTAC 3 cut(s) 13, 282, 293
AfiI CCNNNNNNNGG 3 cut(s) 56, 133, 225
AgsI TTSAA 4 cut(s) 56, 80, 391, 405
AluBI AGCT 3 cut(s) 233, 275, 527
AluI AGCT 3 cut(s) 233, 275, 527
AlwI GGATC 1 cut(s) 263
AoxI GGCC 3 cut(s) 23, 194, 485
ApeKI GCWGC 1 cut(s) 248
Asp700I GAANNNNTTC 1 cut(s) 637
AspA2I CCTAGG 1 cut(s) 208
AsuHPI GGTGA 1 cut(s) 410
AvrII CCTAGG 1 cut(s) 208
BalI TGGCCA 1 cut(s) 487
BbsI GAAGAC 1 cut(s) 640
BbvI GCAGC 1 cut(s) 235
BccI CCATC 1 cut(s) 237
BceAI ACGGC 1 cut(s) 111
BfaI CTAG 2 cut(s) 209, 558
BfmI CTRYAG 1 cut(s) 276
BisI GCNGC 2 cut(s) 23, 249
BlnI CCTAGG 1 cut(s) 208
BlsI GCNGC 2 cut(s) 24, 250
BmcAI AGTACT 1 cut(s) 282
BmrI ACTGGG 2 cut(s) 235, 432
BmsI GCATC 2 cut(s) 450, 521
BmuI ACTGGG 2 cut(s) 235, 432
BpiI GAAGAC 1 cut(s) 640
BpmI CTGGAG 1 cut(s) 321
BpuEI CTTGAG 1 cut(s) 183
BsaJI CCNNGG 1 cut(s) 208
Bsc4I CCNNNNNNNGG 3 cut(s) 56, 133, 225
Bse1I ACTGG 2 cut(s) 230, 427
BseDI CCNNGG 1 cut(s) 208
BseGI GGATG 1 cut(s) 248
BseLI CCNNNNNNNGG 3 cut(s) 56, 133, 225
BseMII CTCAG 1 cut(s) 472
BseNI ACTGG 2 cut(s) 230, 427
BseRI GAGGAG 1 cut(s) 95
BseXI GCAGC 1 cut(s) 235
BshFI GGCC 3 cut(s) 25, 196, 487
BslFI GGGAC 1 cut(s) 112
BslI CCNNNNNNNGG 3 cut(s) 56, 133, 225
BsmFI GGGAC 1 cut(s) 112
BsnI GGCC 3 cut(s) 25, 196, 487
Bsp1407I TGTACA 1 cut(s) 291
Bsp143I GATC 2 cut(s) 268, 358
BspACI CCGC 2 cut(s) 22, 384
BspANI GGCC 3 cut(s) 25, 196, 487
BspCNI CTCAG 1 cut(s) 471
BspHI TCATGA 1 cut(s) 174
BspPI GGATC 1 cut(s) 263
BsrGI TGTACA 1 cut(s) 291
BsrI ACTGG 2 cut(s) 230, 427
BssECI CCNNGG 1 cut(s) 208
BssMI GATC 2 cut(s) 268, 358
BssT1I CCWWGG 1 cut(s) 208
Bst4CI ACNGT 1 cut(s) 544
BstAUI TGTACA 1 cut(s) 291
BstC8I GCNNGC 1 cut(s) 485
BstDEI CTNAG 1 cut(s) 458
BstENI CCTNNNNNAGG 1 cut(s) 54
BstF5I GGATG 1 cut(s) 248
BstKTI GATC 2 cut(s) 271, 361
BstMBI GATC 2 cut(s) 268, 358
BstMWI GCNNNNNNNGC 1 cut(s) 503
BstSFI CTRYAG 1 cut(s) 276
BstV1I GCAGC 1 cut(s) 235
BstV2I GAAGAC 1 cut(s) 640
BsuRI GGCC 3 cut(s) 25, 196, 487
BtsCI GGATG 1 cut(s) 248
BtsI GCAGTG 1 cut(s) 622
BtsIMutI CAGTG 2 cut(s) 466, 622
Cac8I GCNNGC 1 cut(s) 485
CciI TCATGA 1 cut(s) 174
Csp6I GTAC 3 cut(s) 12, 281, 292
CviAII CATG 1 cut(s) 175
CviQI GTAC 3 cut(s) 12, 281, 292
DdeI CTNAG 1 cut(s) 458
DpnI GATC 2 cut(s) 270, 360
DpnII GATC 2 cut(s) 268, 358
EaeI YGGCCR 2 cut(s) 23, 485
Eco130I CCWWGG 1 cut(s) 208
Eco57I CTGAAG 1 cut(s) 383
EcoNI CCTNNNNNAGG 1 cut(s) 54
EcoT14I CCWWGG 1 cut(s) 208
ErhI CCWWGG 1 cut(s) 208
FaeI CATG 1 cut(s) 178
FaqI GGGAC 1 cut(s) 112
FatI CATG 1 cut(s) 174
Fnu4HI GCNGC 2 cut(s) 23, 249
FokI GGATG 1 cut(s) 255
Fsp4HI GCNGC 2 cut(s) 23, 249
FspBI CTAG 2 cut(s) 209, 558
GluI GCNGC 2 cut(s) 23, 249
GsuI CTGGAG 1 cut(s) 321
HaeIII GGCC 3 cut(s) 25, 196, 487
Hin1II CATG 1 cut(s) 178
HincII GTYRAC 2 cut(s) 186, 547
HindII GTYRAC 2 cut(s) 186, 547
HinfI GANTC 4 cut(s) 100, 401, 432, 621
HphI GGTGA 1 cut(s) 410
Hpy166II GTNNAC 2 cut(s) 186, 547
Hpy188I TCNGA 2 cut(s) 74, 363
Hpy188III TCNNGA 4 cut(s) 175, 300, 344, 516
Hpy8I GTNNAC 2 cut(s) 186, 547
HpyCH4III ACNGT 1 cut(s) 544
HpyCH4IV ACGT 1 cut(s) 10
HpyCH4V TGCA 7 cut(s) 251, 338, 441, 497, 512, 570, 615
HpyF10VI GCNNNNNNNGC 1 cut(s) 503
HpyF3I CTNAG 1 cut(s) 458
HpySE526I ACGT 1 cut(s) 10
Hsp92II CATG 1 cut(s) 178
Kzo9I GATC 2 cut(s) 268, 358
LmnI GCTCC 2 cut(s) 230, 261
Lsp1109I GCAGC 1 cut(s) 235
LweI GCATC 2 cut(s) 450, 521
MaeI CTAG 2 cut(s) 209, 558
MaeII ACGT 1 cut(s) 10
MalI GATC 2 cut(s) 270, 360
MboI GATC 2 cut(s) 268, 358
MboII GAAGA 3 cut(s) 365, 376, 645
MfeI CAATTG 2 cut(s) 27, 80
MlsI TGGCCA 1 cut(s) 487
MluCI AATT 6 cut(s) 27, 80, 118, 252, 477, 521
MluNI TGGCCA 1 cut(s) 487
MlyI GAGTC 1 cut(s) 94
MmeI TCCRAC 1 cut(s) 133
MnlI CCTC 4 cut(s) 116, 182, 207, 467
Mox20I TGGCCA 1 cut(s) 487
MroXI GAANNNNTTC 1 cut(s) 637
MscI TGGCCA 1 cut(s) 487
MseI TTAA 3 cut(s) 44, 222, 255
Msp20I TGGCCA 1 cut(s) 487
MunI CAATTG 2 cut(s) 27, 80
MwoI GCNNNNNNNGC 1 cut(s) 503
NdeII GATC 2 cut(s) 268, 358
NlaIII CATG 1 cut(s) 178
PagI TCATGA 1 cut(s) 174
PdmI GAANNNNTTC 1 cut(s) 637
PfeI GAWTC 3 cut(s) 401, 432, 621
PkrI GCNGC 2 cut(s) 24, 250
PleI GAGTC 1 cut(s) 94
PpsI GAGTC 1 cut(s) 94
RsaI GTAC 3 cut(s) 13, 282, 293
RsaNI GTAC 3 cut(s) 12, 281, 292
SaqAI TTAA 3 cut(s) 44, 222, 255
SatI GCNGC 2 cut(s) 23, 249
Sau3AI GATC 2 cut(s) 268, 358
ScaI AGTACT 1 cut(s) 282
SchI GAGTC 1 cut(s) 94
SfaNI GCATC 2 cut(s) 450, 521
SfcI CTRYAG 1 cut(s) 276
SmlI CTYRAG 1 cut(s) 198
SmoI CTYRAG 1 cut(s) 198
Sse9I AATT 6 cut(s) 27, 80, 118, 252, 477, 521
SsiI CCGC 2 cut(s) 22, 384
SspMI CTAG 2 cut(s) 209, 558
StyI CCWWGG 1 cut(s) 208
TaaI ACNGT 1 cut(s) 544
TaiI ACGT 1 cut(s) 13
TaqI TCGA 1 cut(s) 587
TasI AATT 6 cut(s) 27, 80, 118, 252, 477, 521
TatI WGTACW 2 cut(s) 280, 291
TauI GCSGC 1 cut(s) 25
TfiI GAWTC 3 cut(s) 401, 432, 621
Tru1I TTAA 3 cut(s) 44, 222, 255
Tru9I TTAA 3 cut(s) 44, 222, 255
TscAI CASTG 2 cut(s) 466, 622
TseI GCWGC 1 cut(s) 248
TspDTI ATGAA 3 cut(s) 191, 543, 630
TspRI CASTG 2 cut(s) 466, 622
XagI CCTNNNNNAGG 1 cut(s) 54
XmaJI CCTAGG 1 cut(s) 208
XmnI GAANNNNTTC 1 cut(s) 637
XspI CTAG 2 cut(s) 209, 558
ZrmI AGTACT 1 cut(s) 282
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.