MD16G1098700.v1.1

Belongs to the peroxidase family. Classical plant (class III) peroxidase subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0001761
Physical Location & Seq
Forward (+)
6891481 .. 6892745
1265 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1098700.v1.1.491

Sequence Viewer

Length: 1023 bp
ATGCGTCGTCTAATGATCATCGAGACAATCATCTTCACCTTAAGCATCGTTATCGTCGTCATCATCGCTCCTTGTACAGAGGCAGCTGGAAAAGGCCTTGGGAACGGCTTAAATGTAGGTTTCTACAGTCGCAGTTGCCCGAATGTGGAGAAAATTGTGGCTGACATCGTGTCCGAGGCTAACCGGCAAGATCCAAAGCTCCCCGGTGCCCTCATTCGCCTCTTTTCCCATGATTGCTTTGTCAAGGGCTGCGATGCCTCAATTCTGTTGGAGACCACACGCTCAAAGGAGCCTGTAGAGAAAAAAGCACAAGGAAATGACTTCATTAGAGGTTTTGAACTCATTGATGAGATCAAGGCCAGGCTAGAACAAGAGTGCCCGCAAACCGTCTCTTGCGCCGACATACTAGCCTTTGCTGCTCGGGAAGCTGTTTTTCTGGCTGGTCTACCCCGCCACAAAGTCCCCGCTGGACGCCGCGACAGTCGTACTTCCCGTGCTTCCGATGTTGGCCTCCCTGCTCCTACAACACCTTTCAACGATATCATAGATTTCTTCACCAGAAAAGGCATCACCCTCGACGAACTGGTTGTGTTGAGCGGTGCACACTCCATCGGGGTGGCCCATTGTAGCTTCTTTGATTACAGACTCTACAATTTCAACACATCTCAGCCCCAAGACCCTGCCCTCAACTCAACCTACGCTGCTGATCTGTCCACAAAGTGCCCGAAACAAAATACATTGCCTGCAGACGAAGCGAAAAAGAGGGCCGTGGACTTCGATCCAATAACACCACTTGTTCTGGACAACAACTTCTACCTGAACCTTTTGCAAGGGAAAACCTTGCTCCAGTCGGATCAGATAATGGTTTCTGATCCTCGCACCAGCGCGTTGGTAAAGACATTGGCCTCGGATTCTGAAGCTTGGAGTCGAAGGTTTGCCAAGGCCATGATCAAGATGGGGCGAACAAACGTTCTCACTCGAGATGAGGGAGAGATAAGGAAAAATTGCAGAACCTTTAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko01100 ko01110 map00940 map01100 map01110
Pfam Domains
Protein Families
peroxidase

Protein Analysis

341

Amino Acids

37.56

Weight (kDa)

8.32

Isoelectric Point (pI)

44.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
peroxidase PF00141 54 - 304 8.2e-78 Peroxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 206
AccBSI CCGCTC 1 cut(s) 597
AccI GTMKAC 1 cut(s) 445
AccII CGCG 2 cut(s) 477, 887
AciI CCGC 5 cut(s) 380, 451, 465, 475, 597
AclI AACGTT 1 cut(s) 969
AclWI GGATC 4 cut(s) 185, 773, 861, 866
AcuI CTGAAG 1 cut(s) 936
AcyI GRCGYC 1 cut(s) 472
AdeI CACNNNGTG 1 cut(s) 720
AfaI GTAC 2 cut(s) 76, 487
AfiI CCNNNNNNNGG 1 cut(s) 145
AflII CTTAAG 1 cut(s) 40
AgsI TTSAA 3 cut(s) 338, 535, 658
AhdI GACNNNNNGTC 1 cut(s) 169
AjnI CCWGG 1 cut(s) 359
AluBI AGCT 5 cut(s) 86, 199, 428, 630, 920
AluI AGCT 5 cut(s) 86, 199, 428, 630, 920
Alw21I GWGCWC 1 cut(s) 604
Alw26I GTCTC 3 cut(s) 17, 266, 394
Alw44I GTGCAC 1 cut(s) 600
AlwI GGATC 4 cut(s) 185, 773, 861, 866
Ama87I CYCGRG 2 cut(s) 420, 978
AoxI GGCC 7 cut(s) 94, 357, 508, 618, 765, 903, 942
ApaLI GTGCAC 1 cut(s) 600
ApeKI GCWGC 4 cut(s) 83, 249, 416, 701
AspLEI GCGC 2 cut(s) 398, 887
AspS9I GGNCC 2 cut(s) 619, 765
AsuC2I CCSGG 1 cut(s) 204
AsuHPI GGTGA 3 cut(s) 28, 547, 562
AvaI CYCGRG 2 cut(s) 420, 978
BaeGI GKGCMC 4 cut(s) 211, 380, 604, 725
BanI GGYRCC 1 cut(s) 206
Bbv12I GWGCWC 1 cut(s) 604
BbvI GCAGC 4 cut(s) 95, 236, 403, 688
BccI CCATC 2 cut(s) 617, 949
BceAI ACGGC 2 cut(s) 121, 752
BcgI CGANNNNNNTGC 4 cut(s) 34, 68, 556, 590
BciT130I CCWGG 1 cut(s) 361
BclI TGATCA 2 cut(s) 15, 948
BcnI CCSGG 1 cut(s) 204
BcoDI GTCTC 3 cut(s) 17, 266, 394
BfaI CTAG 2 cut(s) 365, 407
BfmI CTRYAG 3 cut(s) 124, 294, 744
BfrI CTTAAG 1 cut(s) 40
BisI GCNGC 5 cut(s) 84, 250, 417, 475, 702
BlsI GCNGC 5 cut(s) 85, 251, 418, 476, 703
Bme1390I CCNGG 2 cut(s) 204, 361
BmeRI GACNNNNNGTC 1 cut(s) 169
BmeT110I CYCGRG 2 cut(s) 420, 978
BmgT120I GGNCC 2 cut(s) 619, 765
BmiI GGNNCC 2 cut(s) 208, 291
BmrFI CCNGG 2 cut(s) 204, 361
BmsI GCATC 3 cut(s) 54, 244, 576
BpmI CTGGAG 1 cut(s) 830
BpuMI CCSGG 1 cut(s) 204
BsaHI GRCGYC 1 cut(s) 472
BsaI GGTCTC 1 cut(s) 266
BsaJI CCNNGG 6 cut(s) 97, 174, 202, 768, 906, 939
Bsc4I CCNNNNNNNGG 1 cut(s) 145
Bse118I RCCGGY 1 cut(s) 183
Bse1I ACTGG 2 cut(s) 588, 847
Bse3DI GCAATG 1 cut(s) 737
BseBI CCWGG 1 cut(s) 361
BseDI CCNNGG 6 cut(s) 97, 174, 202, 768, 906, 939
BseLI CCNNNNNNNGG 1 cut(s) 145
BseMI GCAATG 1 cut(s) 737
BseMII CTCAG 1 cut(s) 680
BseNI ACTGG 2 cut(s) 588, 847
BseSI GKGCMC 4 cut(s) 211, 380, 604, 725
BseXI GCAGC 4 cut(s) 95, 236, 403, 688
Bsh1236I CGCG 2 cut(s) 477, 887
BshFI GGCC 7 cut(s) 96, 359, 510, 620, 767, 905, 944
BshNI GGYRCC 1 cut(s) 206
BsiHKAI GWGCWC 1 cut(s) 604
BsiHKCI CYCGRG 2 cut(s) 420, 978
BsiSI CCGG 2 cut(s) 184, 204
BslFI GGGAC 1 cut(s) 446
BslI CCNNNNNNNGG 1 cut(s) 145
BsmAI GTCTC 3 cut(s) 17, 266, 394
BsmBI CGTCTC 1 cut(s) 394
BsmFI GGGAC 1 cut(s) 446
BsnI GGCC 7 cut(s) 96, 359, 510, 620, 767, 905, 944
Bso31I GGTCTC 1 cut(s) 266
BsoBI CYCGRG 2 cut(s) 420, 978
Bsp1286I GDGCHC 4 cut(s) 211, 380, 604, 725
Bsp1407I TGTACA 1 cut(s) 74
Bsp143I GATC 8 cut(s) 15, 190, 351, 706, 778, 853, 871, 948
BspACI CCGC 5 cut(s) 380, 451, 465, 475, 597
BspANI GGCC 7 cut(s) 96, 359, 510, 620, 767, 905, 944
BspCNI CTCAG 1 cut(s) 679
BspFNI CGCG 2 cut(s) 477, 887
BspLI GGNNCC 2 cut(s) 208, 291
BspMAI CTGCAG 1 cut(s) 748
BspPI GGATC 4 cut(s) 185, 773, 861, 866
BspT107I GGYRCC 1 cut(s) 206
BspTI CTTAAG 1 cut(s) 40
BspTNI GGTCTC 1 cut(s) 266
BsrBI CCGCTC 1 cut(s) 597
BsrDI GCAATG 1 cut(s) 737
BsrFI RCCGGY 1 cut(s) 183
BsrGI TGTACA 1 cut(s) 74
BsrI ACTGG 2 cut(s) 588, 847
BssAI RCCGGY 1 cut(s) 183
BssECI CCNNGG 6 cut(s) 97, 174, 202, 768, 906, 939
BssMI GATC 8 cut(s) 15, 190, 351, 706, 778, 853, 871, 948
BssNI GRCGYC 1 cut(s) 472
BssT1I CCWWGG 2 cut(s) 97, 939
Bst2UI CCWGG 1 cut(s) 361
Bst4CI ACNGT 3 cut(s) 128, 388, 482
BstACI GRCGYC 1 cut(s) 472
BstAFI CTTAAG 1 cut(s) 40
BstAUI TGTACA 1 cut(s) 74
BstC8I GCNNGC 2 cut(s) 380, 744
BstDEI CTNAG 1 cut(s) 666
BstDSI CCRYGG 1 cut(s) 768
BstFNI CGCG 2 cut(s) 477, 887
BstHHI GCGC 2 cut(s) 398, 887
BstKTI GATC 8 cut(s) 18, 193, 354, 709, 781, 856, 874, 951
BstMAI GTCTC 3 cut(s) 17, 266, 394
BstMBI GATC 8 cut(s) 15, 190, 351, 706, 778, 853, 871, 948
BstMWI GCNNNNNNNGC 3 cut(s) 416, 425, 752
BstNI CCWGG 1 cut(s) 361
BstSCI CCNGG 2 cut(s) 202, 359
BstSFI CTRYAG 3 cut(s) 124, 294, 744
BstSLI GKGCMC 4 cut(s) 211, 380, 604, 725
BstUI CGCG 2 cut(s) 477, 887
BstV1I GCAGC 4 cut(s) 95, 236, 403, 688
BstX2I RGATCY 1 cut(s) 190
BstXI CCANNNNNNTGG 2 cut(s) 616, 889
BstYI RGATCY 1 cut(s) 190
BsuRI GGCC 7 cut(s) 96, 359, 510, 620, 767, 905, 944
BtgI CCRYGG 1 cut(s) 768
BtgZI GCGATG 2 cut(s) 49, 267
Cac8I GCNNGC 2 cut(s) 380, 744
CfoI GCGC 2 cut(s) 398, 887
Cfr10I RCCGGY 1 cut(s) 183
Cfr13I GGNCC 2 cut(s) 619, 765
CseI GACGC 1 cut(s) 480
Csp6I GTAC 2 cut(s) 75, 486
CviAII CATG 2 cut(s) 230, 946
CviQI GTAC 2 cut(s) 75, 486
DdeI CTNAG 1 cut(s) 666
DpnI GATC 8 cut(s) 17, 192, 353, 708, 780, 855, 873, 950
DpnII GATC 8 cut(s) 15, 190, 351, 706, 778, 853, 871, 948
DraIII CACNNNGTG 1 cut(s) 720
DriI GACNNNNNGTC 1 cut(s) 169
Eam1105I GACNNNNNGTC 1 cut(s) 169
Eco130I CCWWGG 2 cut(s) 97, 939
Eco147I AGGCCT 1 cut(s) 96
Eco31I GGTCTC 1 cut(s) 266
Eco32I GATATC 1 cut(s) 541
Eco57I CTGAAG 1 cut(s) 936
Eco88I CYCGRG 2 cut(s) 420, 978
EcoRII CCWGG 1 cut(s) 359
EcoRV GATATC 1 cut(s) 541
EcoT14I CCWWGG 2 cut(s) 97, 939
ErhI CCWWGG 2 cut(s) 97, 939
Esp3I CGTCTC 1 cut(s) 394
FaeI CATG 2 cut(s) 233, 949
FaiI YATR 4 cut(s) 231, 404, 545, 947
FaqI GGGAC 1 cut(s) 446
FatI CATG 2 cut(s) 229, 945
FauI CCCGC 3 cut(s) 387, 458, 472
FbaI TGATCA 2 cut(s) 15, 948
FblI GTMKAC 1 cut(s) 445
Fnu4HI GCNGC 5 cut(s) 84, 250, 417, 475, 702
Fsp4HI GCNGC 5 cut(s) 84, 250, 417, 475, 702
FspBI CTAG 2 cut(s) 365, 407
GlaI GCGC 2 cut(s) 397, 886
GluI GCNGC 5 cut(s) 84, 250, 417, 475, 702
GsuI CTGGAG 1 cut(s) 830
HaeIII GGCC 7 cut(s) 96, 359, 510, 620, 767, 905, 944
HapII CCGG 2 cut(s) 184, 204
HgaI GACGC 1 cut(s) 480
HhaI GCGC 2 cut(s) 398, 887
Hin1I GRCGYC 1 cut(s) 472
Hin1II CATG 2 cut(s) 233, 949
Hin6I GCGC 2 cut(s) 396, 885
HinP1I GCGC 2 cut(s) 396, 885
HindIII AAGCTT 1 cut(s) 918
HinfI GANTC 3 cut(s) 645, 911, 925
HpaII CCGG 2 cut(s) 184, 204
HphI GGTGA 3 cut(s) 28, 547, 562
Hpy166II GTNNAC 4 cut(s) 446, 602, 714, 772
Hpy188I TCNGA 7 cut(s) 175, 502, 853, 858, 871, 910, 916
Hpy188III TCNNGA 5 cut(s) 22, 422, 800, 952, 980
Hpy8I GTNNAC 4 cut(s) 446, 602, 714, 772
Hpy99I CGWCG 3 cut(s) 9, 59, 581
HpyAV CCTTC 1 cut(s) 924
HpyCH4III ACNGT 3 cut(s) 128, 388, 482
HpyCH4IV ACGT 1 cut(s) 969
HpyCH4V TGCA 4 cut(s) 602, 746, 829, 1008
HpyF10VI GCNNNNNNNGC 3 cut(s) 416, 425, 752
HpyF3I CTNAG 1 cut(s) 666
HpySE526I ACGT 1 cut(s) 969
Hsp92I GRCGYC 1 cut(s) 472
Hsp92II CATG 2 cut(s) 233, 949
HspAI GCGC 2 cut(s) 396, 885
Ksp22I TGATCA 2 cut(s) 15, 948
Kzo9I GATC 8 cut(s) 15, 190, 351, 706, 778, 853, 871, 948
LmnI GCTCC 5 cut(s) 73, 204, 289, 523, 849
Lsp1109I GCAGC 4 cut(s) 95, 236, 403, 688
LweI GCATC 3 cut(s) 54, 244, 576
MaeI CTAG 2 cut(s) 365, 407
MaeII ACGT 1 cut(s) 969
MalI GATC 8 cut(s) 17, 192, 353, 708, 780, 855, 873, 950
MbiI CCGCTC 1 cut(s) 597
MboI GATC 8 cut(s) 15, 190, 351, 706, 778, 853, 871, 948
MboII GAAGA 2 cut(s) 25, 544
MflI RGATCY 1 cut(s) 190
MhlI GDGCHC 4 cut(s) 211, 380, 604, 725
MluCI AATT 4 cut(s) 153, 261, 652, 1003
MlyI GAGTC 2 cut(s) 639, 934
MmeI TCCRAC 2 cut(s) 249, 831
MseI TTAA 3 cut(s) 41, 110, 1017
MslI CAYNNNNRTG 1 cut(s) 614
MspA1I CMGCKG 2 cut(s) 86, 467
MspCI CTTAAG 1 cut(s) 40
MspI CCGG 2 cut(s) 184, 204
MspR9I CCNGG 2 cut(s) 204, 361
MvaI CCWGG 1 cut(s) 361
MvnI CGCG 2 cut(s) 477, 887
MwoI GCNNNNNNNGC 3 cut(s) 416, 425, 752
NciI CCSGG 1 cut(s) 204
NdeII GATC 8 cut(s) 15, 190, 351, 706, 778, 853, 871, 948
NlaIII CATG 2 cut(s) 233, 949
NlaIV GGNNCC 2 cut(s) 208, 291
PaeR7I CTCGAG 1 cut(s) 978
PceI AGGCCT 1 cut(s) 96
PcsI WCGNNNNNNNCGW 2 cut(s) 54, 490
PfeI GAWTC 1 cut(s) 911
PkrI GCNGC 5 cut(s) 85, 251, 418, 476, 703
PleI GAGTC 2 cut(s) 639, 933
PpsI GAGTC 2 cut(s) 639, 933
Psp1406I AACGTT 1 cut(s) 969
Psp6I CCWGG 1 cut(s) 359
PspGI CCWGG 1 cut(s) 359
PspN4I GGNNCC 2 cut(s) 208, 291
PspPI GGNCC 2 cut(s) 619, 765
PstI CTGCAG 1 cut(s) 748
PsuI RGATCY 1 cut(s) 190
PvuII CAGCTG 1 cut(s) 86
RsaI GTAC 2 cut(s) 76, 487
RsaNI GTAC 2 cut(s) 75, 486
RseI CAYNNNNRTG 1 cut(s) 614
SaqAI TTAA 3 cut(s) 41, 110, 1017
SatI GCNGC 5 cut(s) 84, 250, 417, 475, 702
Sau3AI GATC 8 cut(s) 15, 190, 351, 706, 778, 853, 871, 948
Sau96I GGNCC 2 cut(s) 619, 765
SchI GAGTC 2 cut(s) 639, 934
ScrFI CCNGG 2 cut(s) 204, 361
SduI GDGCHC 4 cut(s) 211, 380, 604, 725
SfaNI GCATC 3 cut(s) 54, 244, 576
SfcI CTRYAG 3 cut(s) 124, 294, 744
Sfr274I CTCGAG 1 cut(s) 978
SlaI CTCGAG 1 cut(s) 978
SmiMI CAYNNNNRTG 1 cut(s) 614
SmlI CTYRAG 2 cut(s) 40, 978
SmoI CTYRAG 2 cut(s) 40, 978
Sse9I AATT 4 cut(s) 153, 261, 652, 1003
SseBI AGGCCT 1 cut(s) 96
SsiI CCGC 5 cut(s) 380, 451, 465, 475, 597
SspMI CTAG 2 cut(s) 365, 407
StuI AGGCCT 1 cut(s) 96
StyD4I CCNGG 2 cut(s) 202, 359
StyI CCWWGG 2 cut(s) 97, 939
TaaI ACNGT 3 cut(s) 128, 388, 482
TaiI ACGT 1 cut(s) 972
TaqI TCGA 5 cut(s) 21, 576, 777, 928, 979
TasI AATT 4 cut(s) 153, 261, 652, 1003
TatI WGTACW 1 cut(s) 74
TauI GCSGC 1 cut(s) 477
TfiI GAWTC 1 cut(s) 911
Tru1I TTAA 3 cut(s) 41, 110, 1017
Tru9I TTAA 3 cut(s) 41, 110, 1017
TseI GCWGC 4 cut(s) 83, 249, 416, 701
TspDTI ATGAA 1 cut(s) 313
Vha464I CTTAAG 1 cut(s) 40
VneI GTGCAC 1 cut(s) 600
XcmI CCANNNNNNNNNTGG 1 cut(s) 952
XhoI CTCGAG 1 cut(s) 978
XmiI GTMKAC 1 cut(s) 445
XspI CTAG 2 cut(s) 365, 407
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.