MD17G1041700.v1.1

Belongs to the MIP aquaporin (TC 1.A.8) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0002869
Physical Location & Seq
Reverse (-)
3047118 .. 3049897
2780 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1041700.v1.1.491

Sequence Viewer

Length: 729 bp
ATGATAGCAACGTATATGTTGGTGTTTGTCACGGCTGGCTCGGCTGCTCTTGCTGCCAGCGACGAACACCAAGTCTCAAAGCTTGGAGCTTCGATTGCAGGAGGGCTCATCATAACCGTGATGATCTATGCGGTCGGCCACATCTCCGGTGCACACATGAACCCGGCCGTCACTCTTGCTTTCGCCGCCGTTAGGCACTTTCCATGGAAACAGGTCCCAATTTATGCGGTAGCTCAAATAGCAGGATCAATATCTGCCTCGTTTACCCTGTATGTACTGTTGCACCCAATTAAACAACTTGGCACCACATCACCTTCTGGATCAGAGACCCAAGCTTTGATCGCAGAGATAATCATGACATTTTCCATGGTGTTCATCGCCTCCGCCGTCGCAACTGATACCAAAGCTGTAGGAGAACTAGCAGGCATCGCAGTTGGCTCCGCGGTTTGCATAACTTCAATCTTTGCCGGACCAATATCAGGTGGATCAATGAACCCAGCAAGAACAATAGGGCCAGCAATCGCTAGTGCATACTACAAAGGAATATGGGTCTACGTCGTTGGACCAGTGATAGGAGCCCTACTTGGCTCATGGTCCTACAGCTTCATTCGGGTCAGCAATAACCCCATCCAGGCAGTATCACCACCCTCATTCACATCCAAGCTTCGTCGAATCAAGACTGACAGCAACGGACAACTTACCTTCAGAGACCCTCTGGATTCAGCTTGA

Protein Analysis

243

Amino Acids

25.09

Weight (kDa)

9.27

Isoelectric Point (pI)

27.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MIP PF00230 2 - 200 9e-52 Major intrinsic protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 302
AccI GTMKAC 1 cut(s) 552
AccII CGCG 1 cut(s) 443
AciI CCGC 6 cut(s) 131, 186, 227, 384, 441, 443
AclWI GGATC 3 cut(s) 253, 328, 493
AcoI YGGCCR 2 cut(s) 136, 165
AcuI CTGAAG 1 cut(s) 688
AfaI GTAC 1 cut(s) 276
AfiI CCNNNNNNNGG 4 cut(s) 192, 479, 572, 631
AgsI TTSAA 1 cut(s) 459
AjnI CCWGG 1 cut(s) 630
AluBI AGCT 8 cut(s) 82, 89, 233, 335, 407, 603, 664, 725
AluI AGCT 8 cut(s) 82, 89, 233, 335, 407, 603, 664, 725
Alw21I GWGCWC 1 cut(s) 154
Alw26I GTCTC 3 cut(s) 79, 320, 702
Alw44I GTGCAC 1 cut(s) 150
AlwI GGATC 3 cut(s) 253, 328, 493
AoxI GGCC 3 cut(s) 136, 165, 512
ApaLI GTGCAC 1 cut(s) 150
ApeKI GCWGC 2 cut(s) 44, 53
ArsI GACNNNNNNTTYG 4 cut(s) 57, 89, 319, 351
AspS9I GGNCC 5 cut(s) 214, 470, 512, 563, 594
AsuC2I CCSGG 1 cut(s) 164
AsuHPI GGTGA 2 cut(s) 303, 633
AvaII GGWCC 4 cut(s) 214, 470, 563, 594
BaeGI GKGCMC 1 cut(s) 154
BanI GGYRCC 1 cut(s) 302
BanII GRGCYC 2 cut(s) 108, 580
Bbv12I GWGCWC 1 cut(s) 154
BbvI GCAGC 2 cut(s) 31, 40
BccI CCATC 1 cut(s) 635
BceAI ACGGC 4 cut(s) 48, 152, 173, 371
BciT130I CCWGG 1 cut(s) 632
BcnI CCSGG 1 cut(s) 164
BcoDI GTCTC 3 cut(s) 79, 320, 702
BfaI CTAG 2 cut(s) 419, 525
BfmI CTRYAG 2 cut(s) 408, 598
BisI GCNGC 3 cut(s) 45, 54, 186
BlsI GCNGC 3 cut(s) 46, 55, 187
Bme1390I CCNGG 2 cut(s) 164, 632
Bme18I GGWCC 4 cut(s) 214, 470, 563, 594
BmgT120I GGNCC 5 cut(s) 214, 470, 512, 563, 594
BmiI GGNNCC 4 cut(s) 216, 304, 439, 577
BmrFI CCNGG 2 cut(s) 164, 632
BmsI GCATC 1 cut(s) 435
BpuMI CCSGG 1 cut(s) 164
BsaBI GATNNNNATC 1 cut(s) 250
BsaI GGTCTC 2 cut(s) 320, 702
BsaJI CCNNGG 3 cut(s) 203, 366, 441
BsaWI WCCGGW 1 cut(s) 146
Bsc4I CCNNNNNNNGG 4 cut(s) 192, 479, 572, 631
Bse1I ACTGG 1 cut(s) 566
Bse8I GATNNNNATC 1 cut(s) 250
BseBI CCWGG 1 cut(s) 632
BseDI CCNNGG 3 cut(s) 203, 366, 441
BseGI GGATG 2 cut(s) 627, 656
BseJI GATNNNNATC 1 cut(s) 250
BseLI CCNNNNNNNGG 4 cut(s) 192, 479, 572, 631
BseNI ACTGG 1 cut(s) 566
BseSI GKGCMC 1 cut(s) 154
BseX3I CGGCCG 1 cut(s) 165
BseXI GCAGC 2 cut(s) 31, 40
BseYI CCCAGC 1 cut(s) 496
Bsh1236I CGCG 1 cut(s) 443
Bsh1285I CGRYCG 2 cut(s) 135, 168
BshFI GGCC 3 cut(s) 138, 167, 514
BshNI GGYRCC 1 cut(s) 302
BsiEI CGRYCG 2 cut(s) 135, 168
BsiHKAI GWGCWC 1 cut(s) 154
BsiSI CCGG 3 cut(s) 147, 164, 468
BslFI GGGAC 1 cut(s) 200
BslI CCNNNNNNNGG 4 cut(s) 192, 479, 572, 631
BsmAI GTCTC 3 cut(s) 79, 320, 702
BsmFI GGGAC 1 cut(s) 200
BsnI GGCC 3 cut(s) 138, 167, 514
Bso31I GGTCTC 2 cut(s) 320, 702
Bsp1286I GDGCHC 3 cut(s) 108, 154, 580
Bsp143I GATC 5 cut(s) 123, 245, 320, 339, 485
Bsp19I CCATGG 2 cut(s) 203, 366
BspACI CCGC 6 cut(s) 131, 186, 227, 384, 441, 443
BspANI GGCC 3 cut(s) 138, 167, 514
BspFNI CGCG 1 cut(s) 443
BspHI TCATGA 1 cut(s) 354
BspLI GGNNCC 4 cut(s) 216, 304, 439, 577
BspPI GGATC 3 cut(s) 253, 328, 493
BspT107I GGYRCC 1 cut(s) 302
BspTNI GGTCTC 2 cut(s) 320, 702
BsrI ACTGG 1 cut(s) 566
BssECI CCNNGG 3 cut(s) 203, 366, 441
BssMI GATC 5 cut(s) 123, 245, 320, 339, 485
BssT1I CCWWGG 2 cut(s) 203, 366
Bst2UI CCWGG 1 cut(s) 632
Bst4CI ACNGT 2 cut(s) 118, 279
BstC8I GCNNGC 4 cut(s) 37, 58, 424, 516
BstDSI CCRYGG 3 cut(s) 203, 366, 441
BstF5I GGATG 2 cut(s) 627, 656
BstFNI CGCG 1 cut(s) 443
BstKTI GATC 5 cut(s) 126, 248, 323, 342, 488
BstMAI GTCTC 3 cut(s) 79, 320, 702
BstMBI GATC 5 cut(s) 123, 245, 320, 339, 485
BstMCI CGRYCG 2 cut(s) 135, 168
BstMWI GCNNNNNNNGC 8 cut(s) 41, 50, 53, 95, 185, 239, 341, 428
BstNI CCWGG 1 cut(s) 632
BstSCI CCNGG 2 cut(s) 162, 630
BstSFI CTRYAG 2 cut(s) 408, 598
BstSLI GKGCMC 1 cut(s) 154
BstUI CGCG 1 cut(s) 443
BstV1I GCAGC 2 cut(s) 31, 40
BstZI CGGCCG 1 cut(s) 165
BsuRI GGCC 3 cut(s) 138, 167, 514
BtgI CCRYGG 3 cut(s) 203, 366, 441
BtgZI GCGATG 2 cut(s) 361, 412
BtsCI GGATG 2 cut(s) 627, 656
BtsIMutI CAGTG 1 cut(s) 573
Cac8I GCNNGC 4 cut(s) 37, 58, 424, 516
CciI TCATGA 1 cut(s) 354
Cfr13I GGNCC 5 cut(s) 214, 470, 512, 563, 594
Cfr42I CCGCGG 1 cut(s) 444
Csp6I GTAC 1 cut(s) 275
CviAII CATG 5 cut(s) 157, 204, 355, 367, 591
CviQI GTAC 1 cut(s) 275
DpnI GATC 5 cut(s) 125, 247, 322, 341, 487
DpnII GATC 5 cut(s) 123, 245, 320, 339, 485
EaeI YGGCCR 2 cut(s) 136, 165
EagI CGGCCG 1 cut(s) 165
EciI GGCGGA 1 cut(s) 373
EclXI CGGCCG 1 cut(s) 165
Eco130I CCWWGG 2 cut(s) 203, 366
Eco24I GRGCYC 2 cut(s) 108, 580
Eco31I GGTCTC 2 cut(s) 320, 702
Eco47I GGWCC 4 cut(s) 214, 470, 563, 594
Eco52I CGGCCG 1 cut(s) 165
Eco57I CTGAAG 1 cut(s) 688
EcoO109I RGGNCCY 1 cut(s) 214
EcoRII CCWGG 1 cut(s) 630
EcoT14I CCWWGG 2 cut(s) 203, 366
EcoT38I GRGCYC 2 cut(s) 108, 580
ErhI CCWWGG 2 cut(s) 203, 366
FaeI CATG 5 cut(s) 160, 207, 358, 370, 594
FaqI GGGAC 1 cut(s) 200
FatI CATG 5 cut(s) 156, 203, 354, 366, 590
FblI GTMKAC 1 cut(s) 552
Fnu4HI GCNGC 3 cut(s) 45, 54, 186
FokI GGATG 2 cut(s) 614, 643
FriOI GRGCYC 2 cut(s) 108, 580
Fsp4HI GCNGC 3 cut(s) 45, 54, 186
FspBI CTAG 2 cut(s) 419, 525
GluI GCNGC 3 cut(s) 45, 54, 186
GsaI CCCAGC 1 cut(s) 500
HaeIII GGCC 3 cut(s) 138, 167, 514
HapII CCGG 3 cut(s) 147, 164, 468
Hin1II CATG 5 cut(s) 160, 207, 358, 370, 594
HindIII AAGCTT 3 cut(s) 80, 333, 662
HinfI GANTC 2 cut(s) 672, 719
HpaII CCGG 3 cut(s) 147, 164, 468
HphI GGTGA 2 cut(s) 303, 633
Hpy166II GTNNAC 3 cut(s) 152, 264, 553
Hpy188I TCNGA 2 cut(s) 325, 707
Hpy188III TCNNGA 4 cut(s) 318, 355, 676, 716
Hpy8I GTNNAC 3 cut(s) 152, 264, 553
Hpy99I CGWCG 4 cut(s) 65, 392, 560, 672
HpyAV CCTTC 2 cut(s) 324, 712
HpyCH4III ACNGT 2 cut(s) 118, 279
HpyCH4IV ACGT 2 cut(s) 11, 555
HpyCH4V TGCA 5 cut(s) 98, 152, 283, 450, 530
HpyF10VI GCNNNNNNNGC 8 cut(s) 41, 50, 53, 95, 185, 239, 341, 428
HpySE526I ACGT 2 cut(s) 11, 555
Hsp92II CATG 5 cut(s) 160, 207, 358, 370, 594
KspI CCGCGG 1 cut(s) 444
Kzo9I GATC 5 cut(s) 123, 245, 320, 339, 485
LmnI GCTCC 3 cut(s) 86, 443, 575
Lsp1109I GCAGC 2 cut(s) 31, 40
LweI GCATC 1 cut(s) 435
MaeI CTAG 2 cut(s) 419, 525
MaeII ACGT 2 cut(s) 11, 555
MaeIII GTNAC 2 cut(s) 28, 169
MalI GATC 5 cut(s) 125, 247, 322, 341, 487
MboI GATC 5 cut(s) 123, 245, 320, 339, 485
MhlI GDGCHC 3 cut(s) 108, 154, 580
MluCI AATT 2 cut(s) 219, 288
MmeI TCCRAC 1 cut(s) 541
MnlI CCTC 5 cut(s) 95, 268, 391, 658, 723
MseI TTAA 1 cut(s) 291
MslI CAYNNNNRTG 1 cut(s) 116
MspA1I CMGCKG 1 cut(s) 443
MspI CCGG 3 cut(s) 147, 164, 468
MspR9I CCNGG 2 cut(s) 164, 632
MvaI CCWGG 1 cut(s) 632
MvnI CGCG 1 cut(s) 443
MwoI GCNNNNNNNGC 8 cut(s) 41, 50, 53, 95, 185, 239, 341, 428
NciI CCSGG 1 cut(s) 164
NcoI CCATGG 2 cut(s) 203, 366
NdeII GATC 5 cut(s) 123, 245, 320, 339, 485
NlaIII CATG 5 cut(s) 160, 207, 358, 370, 594
NlaIV GGNNCC 4 cut(s) 216, 304, 439, 577
NmeAIII GCCGAG 1 cut(s) 20
NmuCI GTSAC 2 cut(s) 28, 169
PagI TCATGA 1 cut(s) 354
PcsI WCGNNNNNNNCGW 1 cut(s) 384
PfeI GAWTC 2 cut(s) 672, 719
PkrI GCNGC 3 cut(s) 46, 55, 187
PpuMI RGGWCCY 1 cut(s) 214
Psp5II RGGWCCY 1 cut(s) 214
Psp6I CCWGG 1 cut(s) 630
PspFI CCCAGC 1 cut(s) 496
PspGI CCWGG 1 cut(s) 630
PspN4I GGNNCC 4 cut(s) 216, 304, 439, 577
PspPI GGNCC 5 cut(s) 214, 470, 512, 563, 594
PspPPI RGGWCCY 1 cut(s) 214
RsaI GTAC 1 cut(s) 276
RsaNI GTAC 1 cut(s) 275
RseI CAYNNNNRTG 1 cut(s) 116
SacII CCGCGG 1 cut(s) 444
SaqAI TTAA 1 cut(s) 291
SatI GCNGC 3 cut(s) 45, 54, 186
Sau3AI GATC 5 cut(s) 123, 245, 320, 339, 485
Sau96I GGNCC 5 cut(s) 214, 470, 512, 563, 594
ScrFI CCNGG 2 cut(s) 164, 632
SduI GDGCHC 3 cut(s) 108, 154, 580
SfaNI GCATC 1 cut(s) 435
SfcI CTRYAG 2 cut(s) 408, 598
Sfr303I CCGCGG 1 cut(s) 444
SgrBI CCGCGG 1 cut(s) 444
SinI GGWCC 4 cut(s) 214, 470, 563, 594
SmiMI CAYNNNNRTG 1 cut(s) 116
Sse9I AATT 2 cut(s) 219, 288
SsiI CCGC 6 cut(s) 131, 186, 227, 384, 441, 443
SspMI CTAG 2 cut(s) 419, 525
StyD4I CCNGG 2 cut(s) 162, 630
StyI CCWWGG 2 cut(s) 203, 366
TaaI ACNGT 2 cut(s) 118, 279
TaiI ACGT 2 cut(s) 14, 558
TaqI TCGA 2 cut(s) 92, 670
TasI AATT 2 cut(s) 219, 288
TatI WGTACW 1 cut(s) 274
TauI GCSGC 1 cut(s) 188
TfiI GAWTC 2 cut(s) 672, 719
Tru1I TTAA 1 cut(s) 291
Tru9I TTAA 1 cut(s) 291
TscAI CASTG 1 cut(s) 573
TseFI GTSAC 2 cut(s) 28, 169
TseI GCWGC 2 cut(s) 44, 53
Tsp45I GTSAC 2 cut(s) 28, 169
TspDTI ATGAA 4 cut(s) 173, 364, 506, 595
TspGWI ACGGA 1 cut(s) 705
TspRI CASTG 1 cut(s) 573
VneI GTGCAC 1 cut(s) 150
VpaK11BI GGWCC 4 cut(s) 214, 470, 563, 594
XmiI GTMKAC 1 cut(s) 552
XspI CTAG 2 cut(s) 419, 525
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.