Rh2BG616100

Belongs to the MIP aquaporin (TC 1.A.8) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0002869
Physical Location & Seq
Forward (+)
83816985 .. 83818285
1301 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG616100.1

Sequence Viewer

Length: 504 bp
ATGGCCCCGACAGAGTTGGTCTCAGTGGAAACCCCAATAGTTGGAAAAGGTCCATTTTACCCACCTGGGTTTTTGAAAAAGGTGGTGGCAGAGATGATAGCTACCTTTCTGCTGGTGTTTGTAACATGTGGTTCAGCTGCTCTTTCAGCGAGTGATGAACGCAAAGTCTCAAAGCTTGGAGCTTCCATGACCGGAGGGCTTATAGTGACGGTGATGATCTACGCAGTTGGGCACATATCTGGGGCACACATGAACCCGGCCGTCACTCTAGCTTTTGCTGCCTTTAGACATTTTCCATGGAAACAGGTGCCAATTTATGCGGTAGCACAACTAACAGGATCAATATCTGCCTCATTTACTCTGTCTCTACTATTGCACCCTATTAAGCACGTGGGCACTACATCACCTTCTGGATCAGAGCTTCGAGCTGTAGTCACAGAGATAATCGTGACATTTTCTAATTTCTATGATGTTCATCACTTCAGCGGTCGCCACCGATACTAA

Protein Analysis

167

Amino Acids

17.89

Weight (kDa)

9.37

Isoelectric Point (pI)

28.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MIP PF00230 23 - 155 2.4e-32 Major intrinsic protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 307
AccB7I CCANNNNNTGG 1 cut(s) 41
AciI CCGC 2 cut(s) 320, 486
AclWI GGATC 2 cut(s) 346, 421
AcoI YGGCCR 1 cut(s) 258
AcuI CTGAAG 1 cut(s) 466
AcvI CACGTG 1 cut(s) 391
AfiI CCNNNNNNNGG 1 cut(s) 41
AflIII ACRYGT 1 cut(s) 125
AgsI TTSAA 1 cut(s) 76
AjnI CCWGG 1 cut(s) 64
AluBI AGCT 7 cut(s) 101, 137, 175, 182, 272, 421, 428
AluI AGCT 7 cut(s) 101, 137, 175, 182, 272, 421, 428
Alw26I GTCTC 3 cut(s) 25, 172, 369
AlwI GGATC 2 cut(s) 346, 421
AoxI GGCC 2 cut(s) 3, 258
ApeKI GCWGC 2 cut(s) 137, 278
AspS9I GGNCC 2 cut(s) 4, 50
AsuC2I CCSGG 1 cut(s) 257
AsuHPI GGTGA 2 cut(s) 223, 396
AvaII GGWCC 1 cut(s) 50
BaeGI GKGCMC 3 cut(s) 234, 247, 398
BanI GGYRCC 1 cut(s) 307
BbrPI CACGTG 1 cut(s) 391
BbvI GCAGC 2 cut(s) 124, 265
BceAI ACGGC 1 cut(s) 245
BciT130I CCWGG 1 cut(s) 66
BcnI CCSGG 1 cut(s) 257
BcoDI GTCTC 3 cut(s) 25, 172, 369
BfaI CTAG 1 cut(s) 269
BfmI CTRYAG 1 cut(s) 429
BisI GCNGC 2 cut(s) 138, 279
BlsI GCNGC 2 cut(s) 139, 280
Bme1390I CCNGG 2 cut(s) 66, 257
Bme18I GGWCC 1 cut(s) 50
BmgT120I GGNCC 2 cut(s) 4, 50
BmiI GGNNCC 2 cut(s) 6, 309
BmrFI CCNGG 2 cut(s) 66, 257
BplI GAGNNNNNCTC 1 cut(s) 37
BpuMI CCSGG 1 cut(s) 257
BsaAI YACGTR 1 cut(s) 391
BsaBI GATNNNNATC 2 cut(s) 343, 474
BsaI GGTCTC 1 cut(s) 25
BsaJI CCNNGG 2 cut(s) 65, 296
BsaWI WCCGGW 1 cut(s) 191
Bsc4I CCNNNNNNNGG 1 cut(s) 41
Bse8I GATNNNNATC 2 cut(s) 343, 474
BseBI CCWGG 1 cut(s) 66
BseDI CCNNGG 2 cut(s) 65, 296
BseJI GATNNNNATC 2 cut(s) 343, 474
BseLI CCNNNNNNNGG 1 cut(s) 41
BseMII CTCAG 1 cut(s) 36
BseSI GKGCMC 3 cut(s) 234, 247, 398
BseX3I CGGCCG 1 cut(s) 258
BseXI GCAGC 2 cut(s) 124, 265
Bsh1285I CGRYCG 2 cut(s) 261, 490
BshFI GGCC 2 cut(s) 5, 260
BshNI GGYRCC 1 cut(s) 307
BsiEI CGRYCG 2 cut(s) 261, 490
BsiSI CCGG 2 cut(s) 192, 257
BslI CCNNNNNNNGG 1 cut(s) 41
BsmAI GTCTC 3 cut(s) 25, 172, 369
BsnI GGCC 2 cut(s) 5, 260
Bso31I GGTCTC 1 cut(s) 25
Bsp1286I GDGCHC 3 cut(s) 234, 247, 398
Bsp143I GATC 3 cut(s) 216, 338, 413
Bsp19I CCATGG 1 cut(s) 296
BspACI CCGC 2 cut(s) 320, 486
BspANI GGCC 2 cut(s) 5, 260
BspCNI CTCAG 1 cut(s) 35
BspLI GGNNCC 2 cut(s) 6, 309
BspPI GGATC 2 cut(s) 346, 421
BspT107I GGYRCC 1 cut(s) 307
BspTNI GGTCTC 1 cut(s) 25
BssECI CCNNGG 2 cut(s) 65, 296
BssMI GATC 3 cut(s) 216, 338, 413
BssT1I CCWWGG 1 cut(s) 296
Bst2UI CCWGG 1 cut(s) 66
Bst4CI ACNGT 1 cut(s) 211
BstBAI YACGTR 1 cut(s) 391
BstDEI CTNAG 1 cut(s) 22
BstDSI CCRYGG 1 cut(s) 296
BstKTI GATC 3 cut(s) 219, 341, 416
BstMAI GTCTC 3 cut(s) 25, 172, 369
BstMBI GATC 3 cut(s) 216, 338, 413
BstMCI CGRYCG 2 cut(s) 261, 490
BstMWI GCNNNNNNNGC 2 cut(s) 146, 278
BstNI CCWGG 1 cut(s) 66
BstNSI RCATGY 1 cut(s) 129
BstSCI CCNGG 2 cut(s) 64, 255
BstSFI CTRYAG 1 cut(s) 429
BstSLI GKGCMC 3 cut(s) 234, 247, 398
BstV1I GCAGC 2 cut(s) 124, 265
BstZI CGGCCG 1 cut(s) 258
BsuRI GGCC 2 cut(s) 5, 260
BtgI CCRYGG 1 cut(s) 296
BtsIMutI CAGTG 1 cut(s) 30
Cfr13I GGNCC 2 cut(s) 4, 50
CviAII CATG 4 cut(s) 126, 187, 250, 297
DdeI CTNAG 1 cut(s) 22
DpnI GATC 3 cut(s) 218, 340, 415
DpnII GATC 3 cut(s) 216, 338, 413
EaeI YGGCCR 1 cut(s) 258
EagI CGGCCG 1 cut(s) 258
EclXI CGGCCG 1 cut(s) 258
Eco130I CCWWGG 1 cut(s) 296
Eco31I GGTCTC 1 cut(s) 25
Eco47I GGWCC 1 cut(s) 50
Eco52I CGGCCG 1 cut(s) 258
Eco57I CTGAAG 1 cut(s) 466
Eco72I CACGTG 1 cut(s) 391
EcoRII CCWGG 1 cut(s) 64
EcoT14I CCWWGG 1 cut(s) 296
ErhI CCWWGG 1 cut(s) 296
FaeI CATG 4 cut(s) 129, 190, 253, 300
FaiI YATR 8 cut(s) 127, 188, 203, 236, 251, 298, 318, 468
FatI CATG 4 cut(s) 125, 186, 249, 296
Fnu4HI GCNGC 2 cut(s) 138, 279
Fsp4HI GCNGC 2 cut(s) 138, 279
FspBI CTAG 1 cut(s) 269
GluI GCNGC 2 cut(s) 138, 279
HaeIII GGCC 2 cut(s) 5, 260
HapII CCGG 2 cut(s) 192, 257
Hin1II CATG 4 cut(s) 129, 190, 253, 300
HindIII AAGCTT 1 cut(s) 173
HpaII CCGG 2 cut(s) 192, 257
HphI GGTGA 2 cut(s) 223, 396
Hpy188I TCNGA 1 cut(s) 418
Hpy188III TCNNGA 2 cut(s) 411, 448
HpyAV CCTTC 1 cut(s) 417
HpyCH4III ACNGT 1 cut(s) 211
HpyCH4IV ACGT 1 cut(s) 390
HpyCH4V TGCA 1 cut(s) 376
HpyF10VI GCNNNNNNNGC 2 cut(s) 146, 278
HpyF3I CTNAG 1 cut(s) 22
HpySE526I ACGT 1 cut(s) 390
Hsp92II CATG 4 cut(s) 129, 190, 253, 300
Kzo9I GATC 3 cut(s) 216, 338, 413
LmnI GCTCC 1 cut(s) 179
LpnPI CCDG 9 cut(s) 51, 78, 98, 205, 225, 270, 290, 321, 396
Lsp1109I GCAGC 2 cut(s) 124, 265
MaeI CTAG 1 cut(s) 269
MaeII ACGT 1 cut(s) 390
MaeIII GTNAC 5 cut(s) 121, 205, 262, 433, 448
MalI GATC 3 cut(s) 218, 340, 415
MboI GATC 3 cut(s) 216, 338, 413
MhlI GDGCHC 3 cut(s) 234, 247, 398
MluCI AATT 2 cut(s) 312, 460
MmeI TCCRAC 1 cut(s) 22
MnlI CCTC 2 cut(s) 188, 361
MseI TTAA 1 cut(s) 384
MspA1I CMGCKG 2 cut(s) 137, 486
MspI CCGG 2 cut(s) 192, 257
MspR9I CCNGG 2 cut(s) 66, 257
MvaI CCWGG 1 cut(s) 66
MwoI GCNNNNNNNGC 2 cut(s) 146, 278
NciI CCSGG 1 cut(s) 257
NcoI CCATGG 1 cut(s) 296
NdeII GATC 3 cut(s) 216, 338, 413
NlaIII CATG 4 cut(s) 129, 190, 253, 300
NlaIV GGNNCC 2 cut(s) 6, 309
NmuCI GTSAC 4 cut(s) 205, 262, 433, 448
NspI RCATGY 1 cut(s) 129
PciI ACATGT 1 cut(s) 125
PflMI CCANNNNNTGG 1 cut(s) 41
PkrI GCNGC 2 cut(s) 139, 280
PmaCI CACGTG 1 cut(s) 391
PmlI CACGTG 1 cut(s) 391
Ppu21I YACGTR 1 cut(s) 391
PscI ACATGT 1 cut(s) 125
Psp6I CCWGG 1 cut(s) 64
PspCI CACGTG 1 cut(s) 391
PspGI CCWGG 1 cut(s) 64
PspN4I GGNNCC 2 cut(s) 6, 309
PspPI GGNCC 2 cut(s) 4, 50
PvuII CAGCTG 1 cut(s) 137
SaqAI TTAA 1 cut(s) 384
SatI GCNGC 2 cut(s) 138, 279
Sau3AI GATC 3 cut(s) 216, 338, 413
Sau96I GGNCC 2 cut(s) 4, 50
ScrFI CCNGG 2 cut(s) 66, 257
SduI GDGCHC 3 cut(s) 234, 247, 398
SfcI CTRYAG 1 cut(s) 429
SinI GGWCC 1 cut(s) 50
Sse9I AATT 2 cut(s) 312, 460
SsiI CCGC 2 cut(s) 320, 486
SspMI CTAG 1 cut(s) 269
StyD4I CCNGG 2 cut(s) 64, 255
StyI CCWWGG 1 cut(s) 296
TaaI ACNGT 1 cut(s) 211
TaiI ACGT 1 cut(s) 393
TaqI TCGA 1 cut(s) 424
TasI AATT 2 cut(s) 312, 460
Tru1I TTAA 1 cut(s) 384
Tru9I TTAA 1 cut(s) 384
TscAI CASTG 1 cut(s) 30
TseFI GTSAC 4 cut(s) 205, 262, 433, 448
TseI GCWGC 2 cut(s) 137, 278
Tsp45I GTSAC 4 cut(s) 205, 262, 433, 448
TspDTI ATGAA 3 cut(s) 171, 266, 464
TspRI CASTG 1 cut(s) 30
Van91I CCANNNNNTGG 1 cut(s) 41
VpaK11BI GGWCC 1 cut(s) 50
XceI RCATGY 1 cut(s) 129
XspI CTAG 1 cut(s) 269
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.