pycom01g02530

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0002049
Physical Location & Seq
Forward (+)
2053307 .. 2055702
2396 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g02530.10

Sequence Viewer

Length: 1695 bp
ATGGCAGAAATAACACCATGTCTCACTCCACTACGCTCACCCACAAATGTAACCTCAGGTAACCAATATGTGCCTAAAATAACATCAAAATTAACAATATCTAACGGGATGAGGTCAGCTGGCATAACAACATCCTCCACCAACACGGGACATCTTGTATACACATACATACTCAAGAGGTGTAGGATGAGGTTGTGTCGTCTGAACAAACATATGAGAAATAACAAAATGCGTATATTCAACGTACCCATCGATCAAATCAGGATTATTCTGAGCATCTTGCAGTGTAATGTGGTGAATATGTCCCTGAACCTGTTGTCGTCCTCCTCGACCTCTGCTAGCCTGATTACCACGTCCCTGCACACTACGCCCCTGACCTGGTTGACTAGGCTGCCTCGATGGTCCTGCACTACTAGAAGCAACCTCCATCTGAATAGGGAGGATACTACGTATAACCTCCAGGATACTGAGGGTAACCACTCTGATAATATGGATCATGCGGATATTGATACTGCTCTACAGAATAAGGAGCTACATCGCCCTGATAATGATAAACGCCCCCACGACCAGTATGGGCATAATTACGAGGTCTTGAAACTTGCTAGATCGGAACTGGTGGTGGTAAAGATGGTGGCTGGGGCCTATGCTGATTCTCATCTGCTACTACCTCGCCTACACTCCCCAAAATGCTTATTATTACATTTGCGACATAAGGGAGTACTTACACTATCAGAACTCCTCTGCCTTTGGAAACGAGGACCTCCAAAAAATCTACCACCTCTCCTCTGCCTAAGGGAACTCAAACCCCTGCTAGAAGAACCAGAACTAACGATTGACCCTTATCTTTACCATTTTTCTTCTGGTTTCCATCTTTTTCTTCCTCCTCACTATCACTGGGCATGTTTTTTGAATCTTCAATTCGAAACAAAATCTCATAAAACTCTTGGTAAGTATACCCAACCTAAAATGACGGAGCATCTATACTGGATTAGCAGCAACCTCCGGGTGATAGTGAGACAAATCAGTAAACCTTCTGTAGTACTCGTTAGCTGTCATCTTCCCCTCTTTTCTGAAATAACTCTGATAAGACTTCTATCTCCACCAGGATGCAACCTCAGGGAGATTCCCTTACCTATGCATCAAACAAAAAGTCTTCTCAAGATGATTGAGCCACCGCTCAGCAACCTCATGTCTTTCATTACCCATAAACTTATCCAACTTCAGATTATACATAGTCTCCAAATGAGTCATCTGAGGAGGGTGAAGCGAAGACTGAATAGCATTCACTCAGTCGAGGAACGTTGCTCTCTAGGAGGTGGCATAGTTTTGACAGAAAACACCAAAATAATTAGAATATTCACAAGATATGCAGGACTACCAAACCTAGGTTCTGATACCAAACTGACACACCTTAATCGAAATCAAGGCTGCGGAAATGATAATGATATGAAGAAATATAAGTGCAAATCCCGATATTTAAGTGTGATACACCATAATCAGAGCATAGGTAACCAGAGGTGGTCTTACATTAGTGATAATCTGTCAGAACCGCCGCGATTCCTTGTAAGCGACCAACAAGCACTCCTACCTAAAACTTGGAGGGGCGCAAAACAGAAAAATTTCCTAGAAATTAAAATACATATCTATATCACAACCATGCTCAGAATAGCAAAATTCACATATATACCATGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

565

Amino Acids

64.86

Weight (kDa)

9.76

Isoelectric Point (pI)

54.34

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 1177
AccI GTMKAC 2 cut(s) 159, 953
AccII CGCG 1 cut(s) 1555
AciI CCGC 5 cut(s) 500, 1175, 1431, 1550, 1553
AclI AACGTT 1 cut(s) 1300
AclWI GGATC 1 cut(s) 501
AcsI RAATTY 2 cut(s) 1618, 1673
AcuI CTGAAG 1 cut(s) 1205
AfaI GTAC 3 cut(s) 246, 720, 1041
AfiI CCNNNNNNNGG 2 cut(s) 378, 1385
AgsI TTSAA 4 cut(s) 241, 595, 910, 917
AjiI CACGTC 1 cut(s) 354
AjnI CCWGG 3 cut(s) 377, 459, 1102
AjuI GAANNNNNNNTTGG 2 cut(s) 1372, 1404
AluBI AGCT 3 cut(s) 119, 532, 1050
AluI AGCT 3 cut(s) 119, 532, 1050
Alw26I GTCTC 3 cut(s) 26, 1009, 1241
AlwI GGATC 1 cut(s) 501
AoxI GGCC 1 cut(s) 639
ApeKI GCWGC 3 cut(s) 391, 993, 1428
ApoI RAATTY 2 cut(s) 1618, 1673
ArsI GACNNNNNNTTYG 2 cut(s) 1135, 1167
Asp700I GAANNNNTTC 1 cut(s) 1619
AspA2I CCTAGG 1 cut(s) 1384
AspLEI GCGC 1 cut(s) 1607
AspS9I GGNCC 3 cut(s) 402, 639, 758
AsuC2I CCSGG 1 cut(s) 1004
AsuHPI GGTGA 4 cut(s) 30, 307, 1018, 1273
AsuII TTCGAA 1 cut(s) 921
AsuNHI GCTAGC 1 cut(s) 338
AvaII GGWCC 2 cut(s) 402, 758
AvrII CCTAGG 1 cut(s) 1384
AxyI CCTNAGG 3 cut(s) 55, 791, 1115
BbsI GAAGAC 2 cut(s) 1145, 1276
BbvI GCAGC 3 cut(s) 378, 1005, 1415
BccI CCATC 5 cut(s) 257, 393, 435, 622, 876
BcgI CGANNNNNNTGC 2 cut(s) 387, 421
BciT130I CCWGG 3 cut(s) 379, 461, 1104
BciVI GTATCC 2 cut(s) 436, 457
BcnI CCSGG 1 cut(s) 1004
BcoDI GTCTC 3 cut(s) 26, 1009, 1241
BfaI CTAG 8 cut(s) 339, 387, 414, 603, 812, 1310, 1385, 1625
BfmI CTRYAG 2 cut(s) 518, 1035
BfuI GTATCC 2 cut(s) 436, 457
BisI GCNGC 4 cut(s) 392, 994, 1429, 1553
BlnI CCTAGG 1 cut(s) 1384
BlpI GCTNAGC 1 cut(s) 1178
BlsI GCNGC 4 cut(s) 393, 995, 1430, 1554
BmcAI AGTACT 2 cut(s) 720, 1041
Bme1390I CCNGG 4 cut(s) 379, 461, 1004, 1104
Bme18I GGWCC 2 cut(s) 402, 758
BmgBI CACGTC 1 cut(s) 354
BmgT120I GGNCC 3 cut(s) 402, 639, 758
BmiI GGNNCC 1 cut(s) 640
BmrFI CCNGG 4 cut(s) 379, 461, 1004, 1104
BmrI ACTGGG 1 cut(s) 904
BmsI GCATC 4 cut(s) 285, 985, 1097, 1147
BmtI GCTAGC 1 cut(s) 342
BmuI ACTGGG 1 cut(s) 904
BpiI GAAGAC 2 cut(s) 1145, 1276
BpmI CTGGAG 1 cut(s) 443
Bpu1102I GCTNAGC 1 cut(s) 1178
Bpu14I TTCGAA 1 cut(s) 921
BpuEI CTTGAG 2 cut(s) 158, 1142
BpuMI CCSGG 1 cut(s) 1004
Bsa29I ATCGAT 1 cut(s) 252
BsaAI YACGTR 1 cut(s) 450
BsaBI GATNNNNATC 1 cut(s) 654
BsaJI CCNNGG 1 cut(s) 1384
BsaXI ACNNNNNCTCC 6 cut(s) 765, 795, 1221, 1251, 1566, 1596
Bsc4I CCNNNNNNNGG 2 cut(s) 378, 1385
Bse1I ACTGG 4 cut(s) 568, 618, 899, 989
Bse21I CCTNAGG 3 cut(s) 55, 791, 1115
Bse8I GATNNNNATC 1 cut(s) 654
BseBI CCWGG 3 cut(s) 379, 461, 1104
BseCI ATCGAT 1 cut(s) 252
BseDI CCNNGG 1 cut(s) 1384
BseGI GGATG 4 cut(s) 114, 131, 192, 1112
BseJI GATNNNNATC 1 cut(s) 654
BseLI CCNNNNNNNGG 2 cut(s) 378, 1385
BseMII CTCAG 8 cut(s) 69, 263, 459, 1129, 1192, 1244, 1302, 1675
BseNI ACTGG 4 cut(s) 568, 618, 899, 989
BseRI GAGGAG 5 cut(s) 316, 728, 773, 873, 1270
BseXI GCAGC 3 cut(s) 378, 1005, 1415
BseYI CCCAGC 1 cut(s) 635
BsgI GTGCAG 2 cut(s) 344, 391
Bsh1236I CGCG 1 cut(s) 1555
BshFI GGCC 1 cut(s) 641
BshVI ATCGAT 1 cut(s) 252
BsiSI CCGG 1 cut(s) 1003
BslFI GGGAC 3 cut(s) 162, 289, 340
BslI CCNNNNNNNGG 2 cut(s) 378, 1385
BsmAI GTCTC 3 cut(s) 26, 1009, 1241
BsmFI GGGAC 3 cut(s) 162, 289, 340
BsmI GAATGC 1 cut(s) 1281
BsnI GGCC 1 cut(s) 641
Bsp119I TTCGAA 1 cut(s) 921
Bsp143I GATC 3 cut(s) 253, 493, 605
Bsp1720I GCTNAGC 1 cut(s) 1178
BspACI CCGC 5 cut(s) 500, 1175, 1431, 1550, 1553
BspANI GGCC 1 cut(s) 641
BspCNI CTCAG 8 cut(s) 68, 264, 460, 1128, 1191, 1245, 1301, 1674
BspDI ATCGAT 1 cut(s) 252
BspFNI CGCG 1 cut(s) 1555
BspLI GGNNCC 1 cut(s) 640
BspOI GCTAGC 1 cut(s) 342
BspPI GGATC 1 cut(s) 501
BspT104I TTCGAA 1 cut(s) 921
BsrBI CCGCTC 1 cut(s) 1177
BsrI ACTGG 4 cut(s) 568, 618, 899, 989
BssECI CCNNGG 1 cut(s) 1384
BssMI GATC 3 cut(s) 253, 493, 605
BssNAI GTATAC 2 cut(s) 160, 954
BssT1I CCWWGG 1 cut(s) 1384
Bst1107I GTATAC 2 cut(s) 160, 954
Bst2UI CCWGG 3 cut(s) 379, 461, 1104
BstBAI YACGTR 1 cut(s) 450
BstBI TTCGAA 1 cut(s) 921
BstC8I GCNNGC 2 cut(s) 121, 340
BstDEI CTNAG 9 cut(s) 55, 272, 468, 791, 1115, 1178, 1253, 1288, 1661
BstEII GGTNACC 3 cut(s) 59, 473, 1508
BstF5I GGATG 4 cut(s) 114, 131, 192, 1112
BstFNI CGCG 1 cut(s) 1555
BstHHI GCGC 1 cut(s) 1607
BstKTI GATC 3 cut(s) 256, 496, 608
BstMAI GTCTC 3 cut(s) 26, 1009, 1241
BstMBI GATC 3 cut(s) 253, 493, 605
BstMWI GCNNNNNNNGC 1 cut(s) 367
BstNI CCWGG 3 cut(s) 379, 461, 1104
BstNSI RCATGY 1 cut(s) 903
BstPI GGTNACC 3 cut(s) 59, 473, 1508
BstSCI CCNGG 4 cut(s) 377, 459, 1002, 1102
BstSFI CTRYAG 2 cut(s) 518, 1035
BstSNI TACGTA 1 cut(s) 450
BstUI CGCG 1 cut(s) 1555
BstV1I GCAGC 3 cut(s) 378, 1005, 1415
BstV2I GAAGAC 2 cut(s) 1145, 1276
BstZ17I GTATAC 2 cut(s) 160, 954
Bsu15I ATCGAT 1 cut(s) 252
Bsu36I CCTNAGG 3 cut(s) 55, 791, 1115
BsuI GTATCC 2 cut(s) 436, 457
BsuRI GGCC 1 cut(s) 641
BsuTUI ATCGAT 1 cut(s) 252
BtgZI GCGATG 1 cut(s) 521
BtrI CACGTC 1 cut(s) 354
BtsCI GGATG 4 cut(s) 114, 131, 192, 1112
BtsI GCAGTG 1 cut(s) 290
BtsIMutI CAGTG 2 cut(s) 290, 892
Cac8I GCNNGC 2 cut(s) 121, 340
CfoI GCGC 1 cut(s) 1607
Cfr13I GGNCC 3 cut(s) 402, 639, 758
ClaI ATCGAT 1 cut(s) 252
CsiI ACCWGGT 1 cut(s) 377
Csp6I GTAC 3 cut(s) 245, 719, 1040
CviAII CATG 6 cut(s) 18, 497, 900, 1189, 1657, 1689
CviJI RGCY 9 cut(s) 119, 342, 391, 532, 635, 641, 1050, 1171, 1428
CviKI_1 RGCY 9 cut(s) 119, 342, 391, 532, 635, 641, 1050, 1171, 1428
CviQI GTAC 3 cut(s) 245, 719, 1040
DdeI CTNAG 9 cut(s) 55, 272, 468, 791, 1115, 1178, 1253, 1288, 1661
DpnI GATC 3 cut(s) 255, 495, 607
DpnII GATC 3 cut(s) 253, 493, 605
Eco105I TACGTA 1 cut(s) 450
Eco130I CCWWGG 1 cut(s) 1384
Eco47I GGWCC 2 cut(s) 402, 758
Eco57I CTGAAG 1 cut(s) 1205
Eco81I CCTNAGG 3 cut(s) 55, 791, 1115
Eco91I GGTNACC 3 cut(s) 59, 473, 1508
EcoO109I RGGNCCY 2 cut(s) 639, 758
EcoO65I GGTNACC 3 cut(s) 59, 473, 1508
EcoRII CCWGG 3 cut(s) 377, 459, 1102
EcoT14I CCWWGG 1 cut(s) 1384
EcoT22I ATGCAT 1 cut(s) 1140
ErhI CCWWGG 1 cut(s) 1384
FaeI CATG 6 cut(s) 21, 500, 903, 1192, 1660, 1692
FaqI GGGAC 3 cut(s) 162, 289, 340
FatI CATG 6 cut(s) 17, 496, 899, 1188, 1656, 1688
FauNDI CATATG 1 cut(s) 213
FblI GTMKAC 2 cut(s) 159, 953
Fnu4HI GCNGC 4 cut(s) 392, 994, 1429, 1553
FokI GGATG 4 cut(s) 118, 121, 199, 1119
Fsp4HI GCNGC 4 cut(s) 392, 994, 1429, 1553
FspBI CTAG 8 cut(s) 339, 387, 414, 603, 812, 1310, 1385, 1625
GlaI GCGC 1 cut(s) 1606
GluI GCNGC 4 cut(s) 392, 994, 1429, 1553
GsaI CCCAGC 1 cut(s) 639
GsuI CTGGAG 1 cut(s) 443
HaeIII GGCC 1 cut(s) 641
HapII CCGG 1 cut(s) 1003
HhaI GCGC 1 cut(s) 1607
Hin1II CATG 6 cut(s) 21, 500, 903, 1192, 1660, 1692
Hin6I GCGC 1 cut(s) 1605
HinP1I GCGC 1 cut(s) 1605
HincII GTYRAC 1 cut(s) 384
HindII GTYRAC 1 cut(s) 384
HinfI GANTC 5 cut(s) 650, 910, 1123, 1246, 1557
HpaII CCGG 1 cut(s) 1003
HphI GGTGA 4 cut(s) 30, 307, 1018, 1273
Hpy166II GTNNAC 4 cut(s) 160, 384, 954, 1028
Hpy188III TCNNGA 5 cut(s) 175, 262, 592, 1159, 1470
Hpy8I GTNNAC 4 cut(s) 160, 384, 954, 1028
HpyAV CCTTC 1 cut(s) 1041
HpyCH4IV ACGT 4 cut(s) 243, 353, 449, 1300
HpyCH4V TGCA 7 cut(s) 283, 361, 408, 1110, 1138, 1370, 1464
HpyF10VI GCNNNNNNNGC 1 cut(s) 367
HpyF3I CTNAG 9 cut(s) 55, 272, 468, 791, 1115, 1178, 1253, 1288, 1661
HpySE526I ACGT 4 cut(s) 243, 353, 449, 1300
Hsp92II CATG 6 cut(s) 21, 500, 903, 1192, 1660, 1692
HspAI GCGC 1 cut(s) 1605
Kzo9I GATC 3 cut(s) 253, 493, 605
LmnI GCTCC 2 cut(s) 529, 973
Lsp1109I GCAGC 3 cut(s) 378, 1005, 1415
LweI GCATC 4 cut(s) 285, 985, 1097, 1147
MabI ACCWGGT 1 cut(s) 377
MaeI CTAG 8 cut(s) 339, 387, 414, 603, 812, 1310, 1385, 1625
MaeII ACGT 4 cut(s) 243, 353, 449, 1300
MaeIII GTNAC 4 cut(s) 49, 59, 473, 1508
MalI GATC 3 cut(s) 255, 495, 607
MbiI CCGCTC 1 cut(s) 1177
MboI GATC 3 cut(s) 253, 493, 605
MboII GAAGA 8 cut(s) 827, 849, 869, 905, 1049, 1145, 1281, 1462
MluCI AATT 7 cut(s) 89, 580, 917, 1347, 1618, 1629, 1673
MlyI GAGTC 1 cut(s) 1255
MmeI TCCRAC 1 cut(s) 1240
Mph1103I ATGCAT 1 cut(s) 1140
MroXI GAANNNNTTC 1 cut(s) 1619
MseI TTAA 5 cut(s) 92, 1413, 1478, 1632, 1693
MslI CAYNNNNRTG 2 cut(s) 1105, 1655
MspA1I CMGCKG 1 cut(s) 119
MspI CCGG 1 cut(s) 1003
MspR9I CCNGG 4 cut(s) 379, 461, 1004, 1104
Mva1269I GAATGC 1 cut(s) 1281
MvaI CCWGG 3 cut(s) 379, 461, 1104
MvnI CGCG 1 cut(s) 1555
MwoI GCNNNNNNNGC 1 cut(s) 367
NciI CCSGG 1 cut(s) 1004
NdeI CATATG 1 cut(s) 213
NdeII GATC 3 cut(s) 253, 493, 605
NheI GCTAGC 1 cut(s) 338
NlaIII CATG 6 cut(s) 21, 500, 903, 1192, 1660, 1692
NlaIV GGNNCC 1 cut(s) 640
NsiI ATGCAT 1 cut(s) 1140
NspI RCATGY 1 cut(s) 903
NspV TTCGAA 1 cut(s) 921
PcsI WCGNNNNNNNCGW 2 cut(s) 249, 326
PctI GAATGC 1 cut(s) 1281
PdmI GAANNNNTTC 1 cut(s) 1619
PfeI GAWTC 4 cut(s) 650, 910, 1123, 1557
PfoI TCCNGGA 1 cut(s) 459
PkrI GCNGC 4 cut(s) 393, 995, 1430, 1554
PleI GAGTC 1 cut(s) 1254
PpsI GAGTC 1 cut(s) 1254
Ppu21I YACGTR 1 cut(s) 450
PpuMI RGGWCCY 1 cut(s) 758
Psp1406I AACGTT 1 cut(s) 1300
Psp5II RGGWCCY 1 cut(s) 758
Psp6I CCWGG 3 cut(s) 377, 459, 1102
PspEI GGTNACC 3 cut(s) 59, 473, 1508
PspFI CCCAGC 1 cut(s) 635
PspGI CCWGG 3 cut(s) 377, 459, 1102
PspN4I GGNNCC 1 cut(s) 640
PspPI GGNCC 3 cut(s) 402, 639, 758
PspPPI RGGWCCY 1 cut(s) 758
PvuII CAGCTG 1 cut(s) 119
RsaI GTAC 3 cut(s) 246, 720, 1041
RsaNI GTAC 3 cut(s) 245, 719, 1040
RseI CAYNNNNRTG 2 cut(s) 1105, 1655
SaqAI TTAA 5 cut(s) 92, 1413, 1478, 1632, 1693
SatI GCNGC 4 cut(s) 392, 994, 1429, 1553
Sau3AI GATC 3 cut(s) 253, 493, 605
Sau96I GGNCC 3 cut(s) 402, 639, 758
ScaI AGTACT 2 cut(s) 720, 1041
SchI GAGTC 1 cut(s) 1255
ScrFI CCNGG 4 cut(s) 379, 461, 1004, 1104
SexAI ACCWGGT 1 cut(s) 377
SfaNI GCATC 4 cut(s) 285, 985, 1097, 1147
SfcI CTRYAG 2 cut(s) 518, 1035
SfuI TTCGAA 1 cut(s) 921
SinI GGWCC 2 cut(s) 402, 758
SmiMI CAYNNNNRTG 2 cut(s) 1105, 1655
SmlI CTYRAG 2 cut(s) 173, 1157
SmoI CTYRAG 2 cut(s) 173, 1157
SnaBI TACGTA 1 cut(s) 450
Sse9I AATT 7 cut(s) 89, 580, 917, 1347, 1618, 1629, 1673
SsiI CCGC 5 cut(s) 500, 1175, 1431, 1550, 1553
SspI AATATT 1 cut(s) 1356
SspMI CTAG 8 cut(s) 339, 387, 414, 603, 812, 1310, 1385, 1625
StyD4I CCNGG 4 cut(s) 377, 459, 1002, 1102
StyI CCWWGG 1 cut(s) 1384
TaiI ACGT 4 cut(s) 246, 356, 452, 1303
TaqI TCGA 6 cut(s) 252, 329, 397, 921, 1293, 1417
TasI AATT 7 cut(s) 89, 580, 917, 1347, 1618, 1629, 1673
TatI WGTACW 2 cut(s) 718, 1039
TauI GCSGC 1 cut(s) 1555
TfiI GAWTC 4 cut(s) 650, 910, 1123, 1557
Tru1I TTAA 5 cut(s) 92, 1413, 1478, 1632, 1693
Tru9I TTAA 5 cut(s) 92, 1413, 1478, 1632, 1693
TscAI CASTG 2 cut(s) 290, 899
TseI GCWGC 3 cut(s) 391, 993, 1428
TspDTI ATGAA 2 cut(s) 1186, 1463
TspGWI ACGGA 1 cut(s) 986
TspRI CASTG 2 cut(s) 290, 899
VpaK11BI GGWCC 2 cut(s) 402, 758
XapI RAATTY 2 cut(s) 1618, 1673
XceI RCATGY 1 cut(s) 903
XcmI CCANNNNNNNNNTGG 2 cut(s) 569, 857
XmaJI CCTAGG 1 cut(s) 1384
XmiI GTMKAC 2 cut(s) 159, 953
XmnI GAANNNNTTC 1 cut(s) 1619
XspI CTAG 8 cut(s) 339, 387, 414, 603, 812, 1310, 1385, 1625
ZrmI AGTACT 2 cut(s) 720, 1041
Zsp2I ATGCAT 1 cut(s) 1140
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.