pycom09g16180

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0002049
Physical Location & Seq
Reverse (-)
16305037 .. 16305393
357 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom09g16180.1

Sequence Viewer

Length: 357 bp
ATGAGCCGATTTAGTGAGTCGATCAACGATCACCCAAATGCCGTCAAAGCCATTTTGTGTACGCGGCAACTTGTACACAAAATCCATGGTGATATTTTCCCATTTCCACTCCGGAACGGGAAGCGGCTGCAACAACCCAAACGGCTTCTTCCTTTCTGCTTTAACTTGCTGACAAACAGCACATCTACTCACATACTCGGCTATCTCCCTCTTCATACCCGGCCAATAATAAAACGGTCGAATGGTATGATACATTTTAGTAGCTCCTGGATGCATGGCATAAGCCGAGATATGTGCTTCATCGAGAATTTCTTTCTTCAACTCCACACTACTAGGCACGAACATTCTACCTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

119

Amino Acids

13.81

Weight (kDa)

10.29

Isoelectric Point (pI)

73.35

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 64
AccIII TCCGGA 1 cut(s) 111
AciI CCGC 2 cut(s) 64, 124
AcoI YGGCCR 1 cut(s) 221
AcsI RAATTY 1 cut(s) 307
AfaI GTAC 2 cut(s) 61, 75
AgsI TTSAA 1 cut(s) 320
AjnI CCWGG 1 cut(s) 266
AluBI AGCT 1 cut(s) 264
AluI AGCT 1 cut(s) 264
Aor13HI TCCGGA 1 cut(s) 111
AoxI GGCC 1 cut(s) 221
ApeKI GCWGC 1 cut(s) 127
ApoI RAATTY 1 cut(s) 307
AsuC2I CCSGG 1 cut(s) 220
AsuHPI GGTGA 2 cut(s) 23, 101
BbvI GCAGC 1 cut(s) 114
BceAI ACGGC 2 cut(s) 26, 158
BcgI CGANNNNNNTGC 2 cut(s) 276, 310
BciT130I CCWGG 1 cut(s) 268
BcnI CCSGG 1 cut(s) 220
BfaI CTAG 2 cut(s) 333, 355
BisI GCNGC 3 cut(s) 65, 125, 128
BlsI GCNGC 3 cut(s) 66, 126, 129
Bme1390I CCNGG 2 cut(s) 220, 268
BmrFI CCNGG 2 cut(s) 220, 268
BmsI GCATC 1 cut(s) 261
BpuMI CCSGG 1 cut(s) 220
BsaJI CCNNGG 1 cut(s) 85
BsaWI WCCGGW 1 cut(s) 111
BseAI TCCGGA 1 cut(s) 111
BseBI CCWGG 1 cut(s) 268
BseDI CCNNGG 1 cut(s) 85
BseGI GGATG 1 cut(s) 276
BseXI GCAGC 1 cut(s) 114
Bsh1236I CGCG 1 cut(s) 64
Bsh1285I CGRYCG 1 cut(s) 239
BshFI GGCC 1 cut(s) 223
BsiEI CGRYCG 1 cut(s) 239
BsiSI CCGG 2 cut(s) 112, 220
BsnI GGCC 1 cut(s) 223
Bsp13I TCCGGA 1 cut(s) 111
Bsp1407I TGTACA 1 cut(s) 73
Bsp143I GATC 2 cut(s) 21, 28
Bsp19I CCATGG 1 cut(s) 85
BspACI CCGC 2 cut(s) 64, 124
BspANI GGCC 1 cut(s) 223
BspEI TCCGGA 1 cut(s) 111
BspFNI CGCG 1 cut(s) 64
BsrGI TGTACA 1 cut(s) 73
BssECI CCNNGG 1 cut(s) 85
BssMI GATC 2 cut(s) 21, 28
BssT1I CCWWGG 1 cut(s) 85
Bst2UI CCWGG 1 cut(s) 268
Bst4CI ACNGT 1 cut(s) 237
Bst6I CTCTTC 1 cut(s) 216
BstAUI TGTACA 1 cut(s) 73
BstDSI CCRYGG 1 cut(s) 85
BstF5I GGATG 1 cut(s) 276
BstFNI CGCG 1 cut(s) 64
BstKTI GATC 2 cut(s) 24, 31
BstMBI GATC 2 cut(s) 21, 28
BstMCI CGRYCG 1 cut(s) 239
BstMWI GCNNNNNNNGC 1 cut(s) 47
BstNI CCWGG 1 cut(s) 268
BstSCI CCNGG 2 cut(s) 218, 266
BstUI CGCG 1 cut(s) 64
BstV1I GCAGC 1 cut(s) 114
BsuRI GGCC 1 cut(s) 223
BtgI CCRYGG 1 cut(s) 85
BtsCI GGATG 1 cut(s) 276
Csp6I GTAC 2 cut(s) 60, 74
CviAII CATG 2 cut(s) 86, 275
CviJI RGCY 8 cut(s) 6, 50, 127, 145, 201, 223, 264, 285
CviKI_1 RGCY 8 cut(s) 6, 50, 127, 145, 201, 223, 264, 285
CviQI GTAC 2 cut(s) 60, 74
DpnI GATC 2 cut(s) 23, 30
DpnII GATC 2 cut(s) 21, 28
EaeI YGGCCR 1 cut(s) 221
Eam1104I CTCTTC 1 cut(s) 216
EarI CTCTTC 1 cut(s) 216
Eco130I CCWWGG 1 cut(s) 85
EcoRII CCWGG 1 cut(s) 266
EcoT14I CCWWGG 1 cut(s) 85
EcoT22I ATGCAT 1 cut(s) 276
ErhI CCWWGG 1 cut(s) 85
FaeI CATG 2 cut(s) 89, 278
FaiI YATR 7 cut(s) 87, 194, 216, 248, 276, 281, 293
FatI CATG 2 cut(s) 85, 274
Fnu4HI GCNGC 3 cut(s) 65, 125, 128
FokI GGATG 1 cut(s) 283
Fsp4HI GCNGC 3 cut(s) 65, 125, 128
FspBI CTAG 2 cut(s) 333, 355
GluI GCNGC 3 cut(s) 65, 125, 128
HaeIII GGCC 1 cut(s) 223
HapII CCGG 2 cut(s) 112, 220
Hin1II CATG 2 cut(s) 89, 278
HinfI GANTC 1 cut(s) 17
HpaII CCGG 2 cut(s) 112, 220
HphI GGTGA 2 cut(s) 23, 101
Hpy166II GTNNAC 2 cut(s) 60, 76
Hpy188III TCNNGA 2 cut(s) 112, 304
Hpy8I GTNNAC 2 cut(s) 60, 76
HpyCH4III ACNGT 1 cut(s) 237
HpyCH4V TGCA 2 cut(s) 130, 274
HpyF10VI GCNNNNNNNGC 1 cut(s) 47
Hsp92II CATG 2 cut(s) 89, 278
Kpn2I TCCGGA 1 cut(s) 111
Kzo9I GATC 2 cut(s) 21, 28
LmnI GCTCC 1 cut(s) 269
LpnPI CCDG 4 cut(s) 125, 233, 253, 280
Lsp1109I GCAGC 1 cut(s) 114
LweI GCATC 1 cut(s) 261
MaeI CTAG 2 cut(s) 333, 355
MalI GATC 2 cut(s) 23, 30
MboI GATC 2 cut(s) 21, 28
MboII GAAGA 3 cut(s) 140, 203, 308
MluCI AATT 1 cut(s) 307
MlyI GAGTC 1 cut(s) 26
MnlI CCTC 1 cut(s) 219
Mph1103I ATGCAT 1 cut(s) 276
MroI TCCGGA 1 cut(s) 111
MseI TTAA 1 cut(s) 162
MslI CAYNNNNRTG 1 cut(s) 36
MspI CCGG 2 cut(s) 112, 220
MspR9I CCNGG 2 cut(s) 220, 268
MvaI CCWGG 1 cut(s) 268
MvnI CGCG 1 cut(s) 64
MwoI GCNNNNNNNGC 1 cut(s) 47
NciI CCSGG 1 cut(s) 220
NcoI CCATGG 1 cut(s) 85
NdeII GATC 2 cut(s) 21, 28
NlaIII CATG 2 cut(s) 89, 278
NmeAIII GCCGAG 2 cut(s) 177, 311
NsiI ATGCAT 1 cut(s) 276
PfoI TCCNGGA 1 cut(s) 266
PkrI GCNGC 3 cut(s) 66, 126, 129
PleI GAGTC 1 cut(s) 25
PpsI GAGTC 1 cut(s) 25
Psp6I CCWGG 1 cut(s) 266
PspGI CCWGG 1 cut(s) 266
RsaI GTAC 2 cut(s) 61, 75
RsaNI GTAC 2 cut(s) 60, 74
RseI CAYNNNNRTG 1 cut(s) 36
SaqAI TTAA 1 cut(s) 162
SatI GCNGC 3 cut(s) 65, 125, 128
Sau3AI GATC 2 cut(s) 21, 28
SchI GAGTC 1 cut(s) 26
ScrFI CCNGG 2 cut(s) 220, 268
SetI ASST 2 cut(s) 266, 353
SfaNI GCATC 1 cut(s) 261
SmiMI CAYNNNNRTG 1 cut(s) 36
Sse9I AATT 1 cut(s) 307
SsiI CCGC 2 cut(s) 64, 124
SspMI CTAG 2 cut(s) 333, 355
StyD4I CCNGG 2 cut(s) 218, 266
StyI CCWWGG 1 cut(s) 85
TaaI ACNGT 1 cut(s) 237
TaqI TCGA 3 cut(s) 20, 239, 303
TasI AATT 1 cut(s) 307
TatI WGTACW 1 cut(s) 73
TauI GCSGC 2 cut(s) 67, 127
Tru1I TTAA 1 cut(s) 162
Tru9I TTAA 1 cut(s) 162
TseI GCWGC 1 cut(s) 127
TspDTI ATGAA 2 cut(s) 203, 289
XapI RAATTY 1 cut(s) 307
XspI CTAG 2 cut(s) 333, 355
Zsp2I ATGCAT 1 cut(s) 276
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.