pycom13g22190

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0002049
Physical Location & Seq
Forward (+)
19338034 .. 19338540
507 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g22190.1

Sequence Viewer

Length: 507 bp
ATGTGCCGACTTAGTAAGCCGATCAACGATCACCCAAATGCCGTCAAAGCCATTATGTGTACGCGGGAGCTTGTACACAAAATCCATGGTGATATTTTCCCATTTCCACTCTGGAACGGGAAGCGGCTGCAACAACCCAAACGGCTTCTTCCTCTCTGCTTTAACCTGCTGACACACAGCACACCTGCTCACATACTCGGCTATCTCTCTCTTCATACCCGGCCAATAATAAAATGGTCGAATGGTATGATACATTTTAGTAGCTCCTGGATGCATGGCATAAGCCGAGATATGTGCTTCATCGAGGATTTCTTTCTTCAACTCCACATTATTAGGCACGAACATTCTACCCTCAAGCATCAACATGCCATCCGAATCACGTACTCTGAGGTCTCGCCTCCTTCCTTGCTCTCGAGCTTGAATAAGTTCTTGAGTCTCCTGATCAACTACCTGAGCTTCAAGCACCCTAAACAAATAGATCGATCGACCAAAATTGGCCTAACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

169

Amino Acids

19.53

Weight (kDa)

9.92

Isoelectric Point (pI)

54.68

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 193
Acc36I ACCTGC 2 cut(s) 174, 193
AccII CGCG 1 cut(s) 64
AciI CCGC 2 cut(s) 64, 124
AcoI YGGCCR 1 cut(s) 221
AfaI GTAC 3 cut(s) 61, 75, 383
AgsI TTSAA 3 cut(s) 320, 421, 460
AjnI CCWGG 1 cut(s) 266
AluBI AGCT 4 cut(s) 70, 264, 417, 456
AluI AGCT 4 cut(s) 70, 264, 417, 456
Alw26I GTCTC 2 cut(s) 397, 440
Ama87I CYCGRG 1 cut(s) 412
AoxI GGCC 2 cut(s) 221, 496
ApeKI GCWGC 1 cut(s) 127
Asp700I GAANNNNTTC 1 cut(s) 425
AsuC2I CCSGG 1 cut(s) 220
AsuHPI GGTGA 2 cut(s) 23, 101
AvaI CYCGRG 1 cut(s) 412
BbvI GCAGC 1 cut(s) 114
BccI CCATC 1 cut(s) 377
BceAI ACGGC 2 cut(s) 26, 158
BcgI CGANNNNNNTGC 2 cut(s) 276, 310
BciT130I CCWGG 1 cut(s) 268
BclI TGATCA 1 cut(s) 441
BcnI CCSGG 1 cut(s) 220
BcoDI GTCTC 2 cut(s) 397, 440
BfuAI ACCTGC 2 cut(s) 174, 193
BisI GCNGC 2 cut(s) 125, 128
BlsI GCNGC 2 cut(s) 126, 129
Bme1390I CCNGG 2 cut(s) 220, 268
BmeT110I CYCGRG 1 cut(s) 412
BmrFI CCNGG 2 cut(s) 220, 268
BmsI GCATC 2 cut(s) 261, 367
Bpu10I CCTNAGC 1 cut(s) 452
BpuEI CTTGAG 2 cut(s) 338, 451
BpuMI CCSGG 1 cut(s) 220
Bsa29I ATCGAT 1 cut(s) 481
BsaAI YACGTR 1 cut(s) 381
BsaI GGTCTC 1 cut(s) 397
BsaJI CCNNGG 1 cut(s) 85
BseBI CCWGG 1 cut(s) 268
BseCI ATCGAT 1 cut(s) 481
BseDI CCNNGG 1 cut(s) 85
BseGI GGATG 2 cut(s) 276, 369
BseMII CTCAG 2 cut(s) 378, 443
BseXI GCAGC 1 cut(s) 114
Bsh1236I CGCG 1 cut(s) 64
Bsh1285I CGRYCG 1 cut(s) 485
BshFI GGCC 2 cut(s) 223, 498
BshVI ATCGAT 1 cut(s) 481
BsiEI CGRYCG 1 cut(s) 485
BsiHKCI CYCGRG 1 cut(s) 412
BsiSI CCGG 1 cut(s) 220
BsmAI GTCTC 2 cut(s) 397, 440
BsnI GGCC 2 cut(s) 223, 498
Bso31I GGTCTC 1 cut(s) 397
BsoBI CYCGRG 1 cut(s) 412
Bsp1407I TGTACA 1 cut(s) 73
Bsp143I GATC 5 cut(s) 21, 28, 441, 478, 482
Bsp19I CCATGG 1 cut(s) 85
BspACI CCGC 2 cut(s) 64, 124
BspANI GGCC 2 cut(s) 223, 498
BspCNI CTCAG 2 cut(s) 379, 444
BspDI ATCGAT 1 cut(s) 481
BspFNI CGCG 1 cut(s) 64
BspMI ACCTGC 2 cut(s) 174, 193
BspTNI GGTCTC 1 cut(s) 397
BsrGI TGTACA 1 cut(s) 73
BssECI CCNNGG 1 cut(s) 85
BssMI GATC 5 cut(s) 21, 28, 441, 478, 482
BssT1I CCWWGG 1 cut(s) 85
Bst2UI CCWGG 1 cut(s) 268
Bst6I CTCTTC 1 cut(s) 216
BstAUI TGTACA 1 cut(s) 73
BstBAI YACGTR 1 cut(s) 381
BstDEI CTNAG 3 cut(s) 11, 387, 452
BstDSI CCRYGG 1 cut(s) 85
BstF5I GGATG 2 cut(s) 276, 369
BstFNI CGCG 1 cut(s) 64
BstKTI GATC 5 cut(s) 24, 31, 444, 481, 485
BstMAI GTCTC 2 cut(s) 397, 440
BstMBI GATC 5 cut(s) 21, 28, 441, 478, 482
BstMCI CGRYCG 1 cut(s) 485
BstMWI GCNNNNNNNGC 1 cut(s) 47
BstNI CCWGG 1 cut(s) 268
BstNSI RCATGY 1 cut(s) 368
BstSCI CCNGG 2 cut(s) 218, 266
BstUI CGCG 1 cut(s) 64
BstV1I GCAGC 1 cut(s) 114
Bsu15I ATCGAT 1 cut(s) 481
BsuRI GGCC 2 cut(s) 223, 498
BsuTUI ATCGAT 1 cut(s) 481
BtgI CCRYGG 1 cut(s) 85
BtsCI GGATG 2 cut(s) 276, 369
BveI ACCTGC 2 cut(s) 174, 193
ClaI ATCGAT 1 cut(s) 481
Csp6I GTAC 3 cut(s) 60, 74, 382
CviAII CATG 3 cut(s) 86, 275, 365
CviQI GTAC 3 cut(s) 60, 74, 382
DdeI CTNAG 3 cut(s) 11, 387, 452
DpnI GATC 5 cut(s) 23, 30, 443, 480, 484
DpnII GATC 5 cut(s) 21, 28, 441, 478, 482
EaeI YGGCCR 1 cut(s) 221
Eam1104I CTCTTC 1 cut(s) 216
EarI CTCTTC 1 cut(s) 216
Eco130I CCWWGG 1 cut(s) 85
Eco31I GGTCTC 1 cut(s) 397
Eco88I CYCGRG 1 cut(s) 412
EcoRII CCWGG 1 cut(s) 266
EcoT14I CCWWGG 1 cut(s) 85
EcoT22I ATGCAT 1 cut(s) 276
ErhI CCWWGG 1 cut(s) 85
FaeI CATG 3 cut(s) 89, 278, 368
FaiI YATR 9 cut(s) 56, 87, 194, 216, 248, 276, 281, 293, 366
FatI CATG 3 cut(s) 85, 274, 364
FauI CCCGC 1 cut(s) 57
FbaI TGATCA 1 cut(s) 441
Fnu4HI GCNGC 2 cut(s) 125, 128
FokI GGATG 2 cut(s) 283, 356
Fsp4HI GCNGC 2 cut(s) 125, 128
GluI GCNGC 2 cut(s) 125, 128
HaeIII GGCC 2 cut(s) 223, 498
HapII CCGG 1 cut(s) 220
Hin1II CATG 3 cut(s) 89, 278, 368
HinfI GANTC 2 cut(s) 375, 433
HpaII CCGG 1 cut(s) 220
HphI GGTGA 2 cut(s) 23, 101
Hpy166II GTNNAC 2 cut(s) 60, 76
Hpy188I TCNGA 2 cut(s) 374, 388
Hpy188III TCNNGA 4 cut(s) 112, 412, 430, 439
Hpy8I GTNNAC 2 cut(s) 60, 76
HpyAV CCTTC 1 cut(s) 411
HpyCH4IV ACGT 1 cut(s) 380
HpyCH4V TGCA 2 cut(s) 130, 274
HpyF10VI GCNNNNNNNGC 1 cut(s) 47
HpyF3I CTNAG 3 cut(s) 11, 387, 452
HpySE526I ACGT 1 cut(s) 380
Hsp92II CATG 3 cut(s) 89, 278, 368
Ksp22I TGATCA 1 cut(s) 441
Kzo9I GATC 5 cut(s) 21, 28, 441, 478, 482
LmnI GCTCC 2 cut(s) 67, 269
LpnPI CCDG 8 cut(s) 97, 179, 198, 233, 253, 280, 452, 464
Lsp1109I GCAGC 1 cut(s) 114
LweI GCATC 2 cut(s) 261, 367
MaeII ACGT 1 cut(s) 380
MalI GATC 5 cut(s) 23, 30, 443, 480, 484
MboI GATC 5 cut(s) 21, 28, 441, 478, 482
MboII GAAGA 3 cut(s) 140, 203, 308
MluCI AATT 1 cut(s) 492
MlyI GAGTC 1 cut(s) 442
MnlI CCTC 5 cut(s) 162, 298, 362, 382, 408
Mph1103I ATGCAT 1 cut(s) 276
MroXI GAANNNNTTC 1 cut(s) 425
MseI TTAA 1 cut(s) 162
MslI CAYNNNNRTG 2 cut(s) 36, 363
MspI CCGG 1 cut(s) 220
MspR9I CCNGG 2 cut(s) 220, 268
MvaI CCWGG 1 cut(s) 268
MvnI CGCG 1 cut(s) 64
MwoI GCNNNNNNNGC 1 cut(s) 47
NciI CCSGG 1 cut(s) 220
NcoI CCATGG 1 cut(s) 85
NdeII GATC 5 cut(s) 21, 28, 441, 478, 482
NlaIII CATG 3 cut(s) 89, 278, 368
NmeAIII GCCGAG 2 cut(s) 177, 311
NsiI ATGCAT 1 cut(s) 276
NspI RCATGY 1 cut(s) 368
PaeR7I CTCGAG 1 cut(s) 412
PaqCI CACCTGC 1 cut(s) 193
PdmI GAANNNNTTC 1 cut(s) 425
PfeI GAWTC 1 cut(s) 375
PfoI TCCNGGA 1 cut(s) 266
PkrI GCNGC 2 cut(s) 126, 129
Ple19I CGATCG 1 cut(s) 485
PleI GAGTC 1 cut(s) 441
PpsI GAGTC 1 cut(s) 441
Ppu21I YACGTR 1 cut(s) 381
Psp6I CCWGG 1 cut(s) 266
PspGI CCWGG 1 cut(s) 266
PvuI CGATCG 1 cut(s) 485
RsaI GTAC 3 cut(s) 61, 75, 383
RsaNI GTAC 3 cut(s) 60, 74, 382
RseI CAYNNNNRTG 2 cut(s) 36, 363
SaqAI TTAA 1 cut(s) 162
SatI GCNGC 2 cut(s) 125, 128
Sau3AI GATC 5 cut(s) 21, 28, 441, 478, 482
SchI GAGTC 1 cut(s) 442
ScrFI CCNGG 2 cut(s) 220, 268
SetI ASST 9 cut(s) 72, 168, 187, 266, 383, 393, 419, 453, 458
SfaNI GCATC 2 cut(s) 261, 367
Sfr274I CTCGAG 1 cut(s) 412
SlaI CTCGAG 1 cut(s) 412
SmiMI CAYNNNNRTG 2 cut(s) 36, 363
SmlI CTYRAG 3 cut(s) 353, 412, 430
SmoI CTYRAG 3 cut(s) 353, 412, 430
Sse9I AATT 1 cut(s) 492
SsiI CCGC 2 cut(s) 64, 124
StyD4I CCNGG 2 cut(s) 218, 266
StyI CCWWGG 1 cut(s) 85
TaiI ACGT 1 cut(s) 383
TaqI TCGA 5 cut(s) 239, 303, 413, 481, 485
TasI AATT 1 cut(s) 492
TatI WGTACW 1 cut(s) 73
TauI GCSGC 1 cut(s) 127
TfiI GAWTC 1 cut(s) 375
Tru1I TTAA 1 cut(s) 162
Tru9I TTAA 1 cut(s) 162
TseI GCWGC 1 cut(s) 127
TspDTI ATGAA 2 cut(s) 203, 289
XceI RCATGY 1 cut(s) 368
XcmI CCANNNNNNNNNTGG 2 cut(s) 108, 231
XhoI CTCGAG 1 cut(s) 412
XmnI GAANNNNTTC 1 cut(s) 425
Zsp2I ATGCAT 1 cut(s) 276
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.